CmoCh08G002350 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G002350
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmembrane protein of ER body-like protein
LocationCmo_Chr08: 1417379 .. 1418827 (-)
RNA-Seq ExpressionCmoCh08G002350
SyntenyCmoCh08G002350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCCGTTATAGATCGGCTGGTGGCGGCGGAAACGGAGAATCAACCGACGCCGGCTGCTGGAGAAAGTAAGTCACGTGCGATGAAGCTGAGCCGAACTTCGTCTTCTGATAGCGATGAAGTTTATTCCGACTCCGACACCGACACCGATGTTGAAAATCAAAGGCTGTACGAGTTACCCAATTCTCACGACTTCTACTGCGCCAACTGCGAATCAAGCATAACTAAAGTGATTATTCTCCGCAACCCTCCGTCGCGGTCGTTGATCTCGGCTGCCAGAGAATCAACCGAGCCACCGCCTAGTTGTTCACCAGACCTGATGTCGACGCTGCAGCCTCGGTATGATACAGATTCAGAAAGCTGTTGCATTTCCGTTGAATGCAGAGGCAATGTGTATATGACCTTTGTATCAAATCGCCCCGAGCCTAGGCGTTGTTTCGAGATAGTGAAGAGCATTGTGTACGGAGGATTAACCGAAGCAATCACGAGCCTAGGCATCGTTACCTCCGCAGCAAGTGCCAATATTCCAATAGGTAAAATTATAATGGACGAGTTTCGGATCATTTGATTATTTTATTATCGTAATTTTGTAGAGGAATGGTCGTGTAATATTAAACTTGGTATGTACAATTTGCAGAGATTATCGGGGCTTTAGCATTGGCAAACATGATTATCGGTCTTATTGTCCTCACAAAAAATGTAAGTCAAACACGTTTTACTTTGATTTATACTCTCAAATTTAAACAGAGTTTAAGAAGTGCTCGTGGGTTGATTTAGGTCGGGTTAAGATATTTTTTAGATCTAACCCAATTGTTCGAGTTATAAAAATTTTCAATTCAACTATAGAGTTTTTGGGTTGAGTTGGTTCGGGTTGACTGATCATTTAATTAAATTTATGTTTTTAATAAAAGTAAAATATTAACCCGAATGGATCTGATTATTTAGGTCAACTGATTTTTTAAACATCTTTGACCTTGAAAACTAGGTTGTTTAGAAAGTATTTACCGGTGCTATGGGAAATGTAATTTTACAGTTCTCTACGCTAAAGTCGAATCAACCCATGAAATACCCATACCGAAGAAAACGACCACAAGTCGATCCATACGAGGAAGCCTTTGGCGACAGGCAGAATTATCTTCTTCACTACTCTGCCATCATTCTATCATTCTTACTCATCGGGCTTCTGCCTCCTCTGGTTTACAGCTTCTCCTTCCGCCACACCGACGACAACAATCTCAAACTCGCCGTTGTGGGTGCCATCTCGCTTCTATGTATTGCGCTACTCGCCATCGCGAAAGCTTATGTTCGGAAGCCGTTGCATAAGTATGTCATAACCCTAGTATGTTACATTGTTCTAGGGTTAGGGGTCTTTGGCCTGTCTTATTTAGGAGGCAAGGTGCTTGGTAAGCTTCTTGAGCCACTTGGCTCGCACTTTGTTGCTCTCTAA

mRNA sequence

ATGGAAGCCGTTATAGATCGGCTGGTGGCGGCGGAAACGGAGAATCAACCGACGCCGGCTGCTGGAGAAAGTAAGTCACGTGCGATGAAGCTGAGCCGAACTTCGTCTTCTGATAGCGATGAAGTTTATTCCGACTCCGACACCGACACCGATGTTGAAAATCAAAGGCTGTACGAGTTACCCAATTCTCACGACTTCTACTGCGCCAACTGCGAATCAAGCATAACTAAAGTGATTATTCTCCGCAACCCTCCGTCGCGGTCGTTGATCTCGGCTGCCAGAGAATCAACCGAGCCACCGCCTAGTTGTTCACCAGACCTGATGTCGACGCTGCAGCCTCGGTATGATACAGATTCAGAAAGCTGTTGCATTTCCGTTGAATGCAGAGGCAATGTGTATATGACCTTTGTATCAAATCGCCCCGAGCCTAGGCGTTGTTTCGAGATAGTGAAGAGCATTGTGTACGGAGGATTAACCGAAGCAATCACGAGCCTAGGCATCGTTACCTCCGCAGCAAGTGCCAATATTCCAATAGAGATTATCGGGGCTTTAGCATTGGCAAACATGATTATCGGTCTTATTGTCCTCACAAAAAATTTCTCTACGCTAAAGTCGAATCAACCCATGAAATACCCATACCGAAGAAAACGACCACAAGTCGATCCATACGAGGAAGCCTTTGGCGACAGGCAGAATTATCTTCTTCACTACTCTGCCATCATTCTATCATTCTTACTCATCGGGCTTCTGCCTCCTCTGGTTTACAGCTTCTCCTTCCGCCACACCGACGACAACAATCTCAAACTCGCCGTTGTGGGTGCCATCTCGCTTCTATGTATTGCGCTACTCGCCATCGCGAAAGCTTATGTTCGGAAGCCGTTGCATAAGTATGTCATAACCCTAGTATGTTACATTGTTCTAGGGTTAGGGGTCTTTGGCCTGTCTTATTTAGGAGGCAAGGTGCTTGGTAAGCTTCTTGAGCCACTTGGCTCGCACTTTGTTGCTCTCTAA

Coding sequence (CDS)

ATGGAAGCCGTTATAGATCGGCTGGTGGCGGCGGAAACGGAGAATCAACCGACGCCGGCTGCTGGAGAAAGTAAGTCACGTGCGATGAAGCTGAGCCGAACTTCGTCTTCTGATAGCGATGAAGTTTATTCCGACTCCGACACCGACACCGATGTTGAAAATCAAAGGCTGTACGAGTTACCCAATTCTCACGACTTCTACTGCGCCAACTGCGAATCAAGCATAACTAAAGTGATTATTCTCCGCAACCCTCCGTCGCGGTCGTTGATCTCGGCTGCCAGAGAATCAACCGAGCCACCGCCTAGTTGTTCACCAGACCTGATGTCGACGCTGCAGCCTCGGTATGATACAGATTCAGAAAGCTGTTGCATTTCCGTTGAATGCAGAGGCAATGTGTATATGACCTTTGTATCAAATCGCCCCGAGCCTAGGCGTTGTTTCGAGATAGTGAAGAGCATTGTGTACGGAGGATTAACCGAAGCAATCACGAGCCTAGGCATCGTTACCTCCGCAGCAAGTGCCAATATTCCAATAGAGATTATCGGGGCTTTAGCATTGGCAAACATGATTATCGGTCTTATTGTCCTCACAAAAAATTTCTCTACGCTAAAGTCGAATCAACCCATGAAATACCCATACCGAAGAAAACGACCACAAGTCGATCCATACGAGGAAGCCTTTGGCGACAGGCAGAATTATCTTCTTCACTACTCTGCCATCATTCTATCATTCTTACTCATCGGGCTTCTGCCTCCTCTGGTTTACAGCTTCTCCTTCCGCCACACCGACGACAACAATCTCAAACTCGCCGTTGTGGGTGCCATCTCGCTTCTATGTATTGCGCTACTCGCCATCGCGAAAGCTTATGTTCGGAAGCCGTTGCATAAGTATGTCATAACCCTAGTATGTTACATTGTTCTAGGGTTAGGGGTCTTTGGCCTGTCTTATTTAGGAGGCAAGGTGCTTGGTAAGCTTCTTGAGCCACTTGGCTCGCACTTTGTTGCTCTCTAA

Protein sequence

MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTDSESCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMIIGLIVLTKNFSTLKSNQPMKYPYRRKRPQVDPYEEAFGDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYVITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL
Homology
BLAST of CmoCh08G002350 vs. ExPASy Swiss-Prot
Match: Q8LPT3 (Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.5e-29
Identity = 80/191 (41.88%), Postives = 117/191 (61.26%), Query Frame = 0

Query: 145 RCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMIIGLIVLTKNFSTLK 204
           R  EI+KSIVYGGL EAITSLG+++SAA +   +  I  L LAN++ GLI++  N   L+
Sbjct: 541 RKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQELR 600

Query: 205 SNQPMK-----YPYRRKRPQVDPYEEAFGDRQNYLLHYSAIILSFLLIGLLPPLVYSFSF 264
             +P++          +  +   Y+   G R+N+ LH +  ILSF++ G+LPP+VY FSF
Sbjct: 601 EEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSF 660

Query: 265 RHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYVITLVCYIVLGLGVFGLSYLGG 324
               + + K+A V   SL CI LLAIAKA+VR P   Y+ +++ Y  + + V G+SY+ G
Sbjct: 661 SEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYPRGSYLKSILYYGSIAVSVSGISYVVG 720

Query: 325 KVLGKLLEPLG 331
             L +LLE  G
Sbjct: 721 NFLEQLLEKHG 731

BLAST of CmoCh08G002350 vs. ExPASy Swiss-Prot
Match: F4KFS7 (Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.5e-26
Identity = 107/385 (27.79%), Postives = 176/385 (45.71%), Query Frame = 0

Query: 12  ETENQPTPAAGESKSRAMKLSRTSSS---------DSDEVYSDSDTDTDVENQRLYELPN 71
           E  N+ + +  E KS   KL  T  +          ++   SDS+  +++EN  L     
Sbjct: 150 EENNERSLSDSEEKSNLEKLLGTQENYELGNEDEEKNERSSSDSEEKSNLEN--LLATQE 209

Query: 72  SHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTL----------- 131
           +++ YC +C + IT+ ++L+       ++++ +     P   PD  S +           
Sbjct: 210 NYELYCPSCSTCITRNVVLKKRKRGKHVNSSLDLKPDIPVVEPDEPSDIEEMESPVKVYV 269

Query: 132 -QPRYDTDSE-------SCCIS------------------------------VECRGNVY 191
            + R + D E       +C +                               ++ R ++ 
Sbjct: 270 PETRIEDDQEDKEGTIFTCLVCDLKYFIRLGTKFLQLDYIRGKPVEKSVEEYIDVRKSIN 329

Query: 192 MTFVSNRPEP---RRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMI 251
            T    + +P   R   E++KS VYGGLTE ITSLG+V+SA+++      I ALA+AN+ 
Sbjct: 330 TTQSPPQIQPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLA 389

Query: 252 IGLIVLTKNFSTLKSNQPMKYPYRRKRPQVDPYEEAFGDRQNYLLHYSAIILSFLLIGLL 311
            GLIVL +NF  L+++            + D YEE  G R    +H    ++S++  GL+
Sbjct: 390 GGLIVLAQNFQDLRNSSDQ---------EKDRYEELLGRRTKSRIHILVAVMSYIFFGLI 449

Query: 312 PPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHK------YVITLVCY 330
           PPLVY+FSF  T   N KL  V   SL+C+ LL   K YVRKP +       Y+ +   Y
Sbjct: 450 PPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYY 509

BLAST of CmoCh08G002350 vs. ExPASy Swiss-Prot
Match: Q8W4P8 (Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.3e-24
Identity = 106/321 (33.02%), Postives = 151/321 (47.04%), Query Frame = 0

Query: 19  PAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYELPNSHDFYCANCESSITKV 78
           P++ E  S+      T + D +    D D D + ENQ+ +  P     Y +  E      
Sbjct: 324 PSSLEKPSKQTVNKETQNHDKEAADPDQDVDKETENQKSHLTP----IYPSPLEQ----- 383

Query: 79  IILRNPPSRSLISAARESTEP--PPSCSPDLMSTLQPRYDTDSESCCISVECRGNVYMTF 138
                 PS+ +I+   + TEP  PP+   ++ ++++PR               GN     
Sbjct: 384 ------PSKQIINKETQ-TEPMLPPNAQSEIPNSVEPRKG-------------GNK---- 443

Query: 139 VSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMIIGLIVL 198
                      EI+KSIVYGGLTE+ITSL  VTSAA++      + AL +AN+  GL++ 
Sbjct: 444 ----------VEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLT 503

Query: 199 TKNFSTLKSNQPMKYPYRRKRP-----QVDPYEEAFGDRQNYLLHYSAIILSFLLIGLLP 258
             +   L + +P K       P     + D YEE  G R+   +H    I SF++ GL+P
Sbjct: 504 VHSLQELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIP 563

Query: 259 PLVYSFSFRHTDD--NNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYVITLVCYIVLGL 318
           PLVY FSFR   +     K+  V A+SLLCI LL+IAKAYV K    YV TL  Y     
Sbjct: 564 PLVYGFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKK-RDYVKTLFRYTTTAT 600

Query: 319 GVFGLSYLGGKVLGKLLEPLG 331
              G S   G ++ + LE  G
Sbjct: 624 TASGFSQFVGYLVSQWLEKSG 600

BLAST of CmoCh08G002350 vs. ExPASy TrEMBL
Match: A0A6J1H7C4 (membrane protein of ER body-like protein OS=Cucurbita moschata OX=3662 GN=LOC111461142 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 7.0e-179
Identity = 336/349 (96.28%), Postives = 336/349 (96.28%), Query Frame = 0

Query: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYEL 60
           MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYEL
Sbjct: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYEL 60

Query: 61  PNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTDSE 120
           PNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTDSE
Sbjct: 61  PNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTDSE 120

Query: 121 SCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEI 180
           SCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEI
Sbjct: 121 SCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEI 180

Query: 181 IGALALANMIIGLIVLTKN-------------FSTLKSNQPMKYPYRRKRPQVDPYEEAF 240
           IGALALANMIIGLIVLTKN             FSTLKSNQPMKYPYRRKRPQVDPYEEAF
Sbjct: 181 IGALALANMIIGLIVLTKNKVFTGAMGNVILQFSTLKSNQPMKYPYRRKRPQVDPYEEAF 240

Query: 241 GDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAK 300
           GDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAK
Sbjct: 241 GDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAK 300

Query: 301 AYVRKPLHKYVITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL 337
           AYVRKPLHKYVITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL
Sbjct: 301 AYVRKPLHKYVITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL 349

BLAST of CmoCh08G002350 vs. ExPASy TrEMBL
Match: A0A6J1KVU9 (membrane protein of ER body 2-like OS=Cucurbita maxima OX=3661 GN=LOC111498065 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 1.1e-83
Identity = 170/195 (87.18%), Postives = 176/195 (90.26%), Query Frame = 0

Query: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDT--DVENQRLY 60
           MEAVID  V  ET+NQ TPA GESKSRA  LSRTSSSD DEVYSD+DTDT  DVENQRLY
Sbjct: 1   MEAVIDLPVVVETDNQLTPADGESKSRAKTLSRTSSSDRDEVYSDTDTDTDADVENQRLY 60

Query: 61  ELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTD 120
           ELPNSHDFYC NC+SSITKVIILRNPPS SLIS A +STEPPPSCSPDLM  LQPRYDTD
Sbjct: 61  ELPNSHDFYCTNCKSSITKVIILRNPPSWSLISTAGKSTEPPPSCSPDLMPKLQPRYDTD 120

Query: 121 SESCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPI 180
           SESCCIS+ECRGNVYMTFVS+RPEPR CFEIVKSIVYGGLTEAITSLGIVTS ASANIPI
Sbjct: 121 SESCCISIECRGNVYMTFVSSRPEPRHCFEIVKSIVYGGLTEAITSLGIVTSGASANIPI 180

Query: 181 EIIGALALANMIIGL 194
           EIIGALALANMIIGL
Sbjct: 181 EIIGALALANMIIGL 195

BLAST of CmoCh08G002350 vs. ExPASy TrEMBL
Match: A0A6J1GPB8 (membrane protein of ER body 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455908 PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 1.6e-58
Identity = 162/351 (46.15%), Postives = 191/351 (54.42%), Query Frame = 0

Query: 49  DTDVENQRLYELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEP----PPSCS 108
           + +++ Q LY +P+SH+FYC  C   ITKVII R+PP        R    P    P S  
Sbjct: 114 EQELDLQTLYNIPDSHNFYCPTCNCCITKVIIRRDPP------LPRSDVIPPEIRPQSPG 173

Query: 109 PDLMST-LQPR-YDTDSESC---------CISVECRGNVYMTFVSNRPEPR--------- 168
           PD   T ++PR ++ D + C         C S       +M  +    +P+         
Sbjct: 174 PDAGGTDVRPRKFNPDDDGCGRIDVLCSNCFSFLAPIGKWMASLFRPEKPQIPDEKGTDT 233

Query: 169 -------------------------------------------RCFEIVKSIVYGGLTEA 228
                                                      R  EI+KSIVYGGLTEA
Sbjct: 234 TGGAGPSTTPGEPDIKDERPSSFIIPIPPGDGGERFGQTVSRGRSLEIMKSIVYGGLTEA 293

Query: 229 ITSLGIVTSAASANIPIEIIGALALANMIIGLIVLTKNFSTLKSNQPMKYPYRRKRPQVD 288
           ITSLGIVTSAASA+ P E I ALALAN+I GLIV+T N S LKSNQ  K P  R   +VD
Sbjct: 294 ITSLGIVTSAASASTPTENIVALALANLITGLIVITNNISGLKSNQLKKEPNERGEAKVD 353

Query: 289 PYEEAFGDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIA 331
            YEEA GDRQ+YLLHY+  ILSFL+ GLLPPLVY FSFR TDD +LKLA V A SLLCIA
Sbjct: 354 SYEEALGDRQHYLLHYTIAILSFLIFGLLPPLVYGFSFRDTDDGDLKLAAVAASSLLCIA 413

BLAST of CmoCh08G002350 vs. ExPASy TrEMBL
Match: A0A6J1GPB5 (membrane protein of ER body 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455908 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 3.6e-58
Identity = 162/354 (45.76%), Postives = 191/354 (53.95%), Query Frame = 0

Query: 49  DTDVENQRLYELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEP----PPSCS 108
           + +++ Q LY +P+SH+FYC  C   ITKVII R+PP        R    P    P S  
Sbjct: 114 EQELDLQTLYNIPDSHNFYCPTCNCCITKVIIRRDPP------LPRSDVIPPEIRPQSPG 173

Query: 109 PDLMST-LQPR-YDTDSESC---------CISVECRGNVYMTFVSNRPEPR--------- 168
           PD   T ++PR ++ D + C         C S       +M  +    +P+         
Sbjct: 174 PDAGGTDVRPRKFNPDDDGCGRIDVLCSNCFSFLAPIGKWMASLFRPEKPQIPDEKAPPG 233

Query: 169 ----------------------------------------------RCFEIVKSIVYGGL 228
                                                         R  EI+KSIVYGGL
Sbjct: 234 TDTTGGAGPSTTPGEPDIKDERPSSFIIPIPPGDGGERFGQTVSRGRSLEIMKSIVYGGL 293

Query: 229 TEAITSLGIVTSAASANIPIEIIGALALANMIIGLIVLTKNFSTLKSNQPMKYPYRRKRP 288
           TEAITSLGIVTSAASA+ P E I ALALAN+I GLIV+T N S LKSNQ  K P  R   
Sbjct: 294 TEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNISGLKSNQLKKEPNERGEA 353

Query: 289 QVDPYEEAFGDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLL 331
           +VD YEEA GDRQ+YLLHY+  ILSFL+ GLLPPLVY FSFR TDD +LKLA V A SLL
Sbjct: 354 KVDSYEEALGDRQHYLLHYTIAILSFLIFGLLPPLVYGFSFRDTDDGDLKLAAVAASSLL 413

BLAST of CmoCh08G002350 vs. ExPASy TrEMBL
Match: A0A6J1JXX6 (membrane protein of ER body-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488504 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.1e-57
Identity = 160/347 (46.11%), Postives = 191/347 (55.04%), Query Frame = 0

Query: 49  DTDVENQRLYELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLM 108
           + +++ Q LY+LP+SH+FYC  C+  ITKVII R+PPS    S    +   P S  PD  
Sbjct: 114 EQELDLQTLYKLPDSHNFYCPTCKCCITKVIIRRDPPSPR--SDVTPTEIRPRSPGPDAG 173

Query: 109 ST------LQPRYDTDSE-----SCCISVECRGNVYMTFVSNRPEPR------------- 168
            T        P  D         S C S       +M+ +  R +P+             
Sbjct: 174 RTDVRDDVFNPEGDHGGRIDVLCSNCFSFLAPIGKWMSSLFRREKPQFPAEKGTDTTSGA 233

Query: 169 ---------------------------------------RCFEIVKSIVYGGLTEAITSL 228
                                                  R  EI+KSIVYGGLTEAITSL
Sbjct: 234 GPSTTPGEPDIKDEGPPSFVIPIPPGDGGERLGQTVSGGRSLEIMKSIVYGGLTEAITSL 293

Query: 229 GIVTSAASANIPIEIIGALALANMIIGLIVLTKNFSTLKSNQPMKYPYRRKRPQVDPYEE 288
           GIVTSAASA+ P E I ALALAN+I GLIV+T N S LKSN+  K P  R   +VD YEE
Sbjct: 294 GIVTSAASASTPTENIVALALANLITGLIVITNNISGLKSNKLKKEPNERGEAKVDSYEE 353

Query: 289 AFGDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAI 331
           A GDRQ+YLLH++  +LSFL+ GLLPPLVY FSFR+TDD +LKLA V A SLLCIALLAI
Sbjct: 354 ALGDRQHYLLHFTIAMLSFLIFGLLPPLVYGFSFRNTDDGDLKLAAVAASSLLCIALLAI 413

BLAST of CmoCh08G002350 vs. NCBI nr
Match: XP_022960407.1 (membrane protein of ER body-like protein [Cucurbita moschata])

HSP 1 Score: 636.3 bits (1640), Expect = 1.5e-178
Identity = 336/349 (96.28%), Postives = 336/349 (96.28%), Query Frame = 0

Query: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYEL 60
           MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYEL
Sbjct: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYEL 60

Query: 61  PNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTDSE 120
           PNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTDSE
Sbjct: 61  PNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTDSE 120

Query: 121 SCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEI 180
           SCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEI
Sbjct: 121 SCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEI 180

Query: 181 IGALALANMIIGLIVLTKN-------------FSTLKSNQPMKYPYRRKRPQVDPYEEAF 240
           IGALALANMIIGLIVLTKN             FSTLKSNQPMKYPYRRKRPQVDPYEEAF
Sbjct: 181 IGALALANMIIGLIVLTKNKVFTGAMGNVILQFSTLKSNQPMKYPYRRKRPQVDPYEEAF 240

Query: 241 GDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAK 300
           GDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAK
Sbjct: 241 GDRQNYLLHYSAIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAK 300

Query: 301 AYVRKPLHKYVITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL 337
           AYVRKPLHKYVITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL
Sbjct: 301 AYVRKPLHKYVITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL 349

BLAST of CmoCh08G002350 vs. NCBI nr
Match: KAG6593019.1 (Membrane protein of ER body 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 547.4 bits (1409), Expect = 8.9e-152
Identity = 296/338 (87.57%), Postives = 307/338 (90.83%), Query Frame = 0

Query: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTD--VENQRLY 60
           MEAV+DR VAAETENQPTPAAGESKSRAMKLSRTSS DSDEVYSD+DTDTD  VENQRLY
Sbjct: 1   MEAVVDRPVAAETENQPTPAAGESKSRAMKLSRTSSFDSDEVYSDTDTDTDVNVENQRLY 60

Query: 61  ELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTD 120
           ELPNS+DFYC NC S+I+KVIILRNPPS  LISAARES     SCSPDL+  LQPR  + 
Sbjct: 61  ELPNSNDFYCPNCVSNISKVIILRNPPSPLLISAARES-----SCSPDLIPKLQPR--SH 120

Query: 121 SESCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPI 180
            E C IS +C GNVY+TFVS+RPEPR CFEIVKSIVYGGLTEAITSLGIVTSAASANIPI
Sbjct: 121 VELCSISAQCSGNVYITFVSSRPEPRYCFEIVKSIVYGGLTEAITSLGIVTSAASANIPI 180

Query: 181 EIIGALALANMIIGLIVLTKNFSTLKSNQPMKYPYRRKRPQVDPYEEAFGDRQNYLLHYS 240
           EI GAL LANMIIGLIVLTKNFSTLKSNQP KYPYRRKRPQVDPYEEAFGDRQNYLLHYS
Sbjct: 181 EITGALVLANMIIGLIVLTKNFSTLKSNQPKKYPYRRKRPQVDPYEEAFGDRQNYLLHYS 240

Query: 241 AIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYV 300
           AIILSFLLIGLLPPLVYSFSFRHTD NNLKLA+VG  SLLCIALLAIAKAYVRKPLHKYV
Sbjct: 241 AIILSFLLIGLLPPLVYSFSFRHTDGNNLKLAIVGVTSLLCIALLAIAKAYVRKPLHKYV 300

Query: 301 ITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL 337
            TLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL
Sbjct: 301 KTLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL 331

BLAST of CmoCh08G002350 vs. NCBI nr
Match: XP_023514589.1 (membrane protein of ER body-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 533.1 bits (1372), Expect = 1.7e-147
Identity = 288/332 (86.75%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDS--DTDTDVENQRLY 60
           MEAVIDR VAAETENQ T AAGESKSRAMKLSRTSSSDSDEVYSDS  DTDTDVENQRLY
Sbjct: 1   MEAVIDRPVAAETENQSTLAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDTDVENQRLY 60

Query: 61  ELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTD 120
           ELPNSHDFYCANCESSITKVIILRNPP RSLISAA ES     SCSP+L+  LQPR  + 
Sbjct: 61  ELPNSHDFYCANCESSITKVIILRNPPLRSLISAAGES-----SCSPNLIPKLQPR--SH 120

Query: 121 SESCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPI 180
           +E C IS +C GNVY+TFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPI
Sbjct: 121 AEICSISAQCSGNVYITFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPI 180

Query: 181 EIIGALALANMIIGLIVLTKNFSTLKSNQPMKYPYRRKRPQVDPYEEAFGDRQNYLLHYS 240
           EIIGALALANMIIGLIVLTKNFSTLKSNQP KYPY+RKRPQVDPYEEA GDRQNYLLHY+
Sbjct: 181 EIIGALALANMIIGLIVLTKNFSTLKSNQPKKYPYQRKRPQVDPYEEALGDRQNYLLHYT 240

Query: 241 AIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYV 300
           A+ILSFLLIG LPPL+Y FSFRHTD +NLKLA V A SLLCIALLA+AKAYVRKPLHKYV
Sbjct: 241 AVILSFLLIGFLPPLIYGFSFRHTDGSNLKLAAVAATSLLCIALLAVAKAYVRKPLHKYV 300

Query: 301 ITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLG 331
            TLVCYIVLGLGVFGLSY+ GKVLGKLLEPLG
Sbjct: 301 KTLVCYIVLGLGVFGLSYVAGKVLGKLLEPLG 325

BLAST of CmoCh08G002350 vs. NCBI nr
Match: XP_023004885.1 (membrane protein of ER body 2-like [Cucurbita maxima])

HSP 1 Score: 320.1 bits (819), Expect = 2.3e-83
Identity = 170/195 (87.18%), Postives = 176/195 (90.26%), Query Frame = 0

Query: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDT--DVENQRLY 60
           MEAVID  V  ET+NQ TPA GESKSRA  LSRTSSSD DEVYSD+DTDT  DVENQRLY
Sbjct: 1   MEAVIDLPVVVETDNQLTPADGESKSRAKTLSRTSSSDRDEVYSDTDTDTDADVENQRLY 60

Query: 61  ELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTD 120
           ELPNSHDFYC NC+SSITKVIILRNPPS SLIS A +STEPPPSCSPDLM  LQPRYDTD
Sbjct: 61  ELPNSHDFYCTNCKSSITKVIILRNPPSWSLISTAGKSTEPPPSCSPDLMPKLQPRYDTD 120

Query: 121 SESCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPI 180
           SESCCIS+ECRGNVYMTFVS+RPEPR CFEIVKSIVYGGLTEAITSLGIVTS ASANIPI
Sbjct: 121 SESCCISIECRGNVYMTFVSSRPEPRHCFEIVKSIVYGGLTEAITSLGIVTSGASANIPI 180

Query: 181 EIIGALALANMIIGL 194
           EIIGALALANMIIGL
Sbjct: 181 EIIGALALANMIIGL 195

BLAST of CmoCh08G002350 vs. NCBI nr
Match: KAG7025428.1 (Membrane protein of ER body-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 308.5 bits (789), Expect = 6.9e-80
Identity = 189/338 (55.92%), Postives = 197/338 (58.28%), Query Frame = 0

Query: 1   MEAVIDRLVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTD--VENQRLY 60
           MEAV+DR VAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSD+DTDTD  VENQRLY
Sbjct: 1   MEAVVDRPVAAETENQPTPAAGESKSRAMKLSRTSSSDSDEVYSDTDTDTDVNVENQRLY 60

Query: 61  ELPNSHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTLQPRYDTD 120
           ELPNS+DFYC NC S+I+++                                        
Sbjct: 61  ELPNSNDFYCPNCVSNISRI---------------------------------------- 120

Query: 121 SESCCISVECRGNVYMTFVSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPI 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 EIIGALALANMIIGLIVLTKNFSTLKSNQPMKYPYRRKRPQVDPYEEAFGDRQNYLLHYS 240
                                        P   PYRRKRPQVDPYEEAFGDRQNYLLHY+
Sbjct: 181 ----------------------------NPRNTPYRRKRPQVDPYEEAFGDRQNYLLHYT 210

Query: 241 AIILSFLLIGLLPPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYV 300
           AIILSFLLIGLLPPLVYSFSFRHTD NNLKLAVVG  SLLCIALLAIAKAYVRKPLHKYV
Sbjct: 241 AIILSFLLIGLLPPLVYSFSFRHTDGNNLKLAVVGVTSLLCIALLAIAKAYVRKPLHKYV 210

Query: 301 ITLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL 337
            TLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL
Sbjct: 301 KTLVCYIVLGLGVFGLSYLGGKVLGKLLEPLGSHFVAL 210

BLAST of CmoCh08G002350 vs. TAIR 10
Match: AT4G27870.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.1e-30
Identity = 80/191 (41.88%), Postives = 117/191 (61.26%), Query Frame = 0

Query: 145 RCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMIIGLIVLTKNFSTLK 204
           R  EI+KSIVYGGL EAITSLG+++SAA +   +  I  L LAN++ GLI++  N   L+
Sbjct: 541 RKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQELR 600

Query: 205 SNQPMK-----YPYRRKRPQVDPYEEAFGDRQNYLLHYSAIILSFLLIGLLPPLVYSFSF 264
             +P++          +  +   Y+   G R+N+ LH +  ILSF++ G+LPP+VY FSF
Sbjct: 601 EEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSF 660

Query: 265 RHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYVITLVCYIVLGLGVFGLSYLGG 324
               + + K+A V   SL CI LLAIAKA+VR P   Y+ +++ Y  + + V G+SY+ G
Sbjct: 661 SEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYPRGSYLKSILYYGSIAVSVSGISYVVG 720

Query: 325 KVLGKLLEPLG 331
             L +LLE  G
Sbjct: 721 NFLEQLLEKHG 731

BLAST of CmoCh08G002350 vs. TAIR 10
Match: AT5G24290.2 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 120.6 bits (301), Expect = 2.5e-27
Identity = 107/385 (27.79%), Postives = 176/385 (45.71%), Query Frame = 0

Query: 12  ETENQPTPAAGESKSRAMKLSRTSSS---------DSDEVYSDSDTDTDVENQRLYELPN 71
           E  N+ + +  E KS   KL  T  +          ++   SDS+  +++EN  L     
Sbjct: 134 EENNERSLSDSEEKSNLEKLLGTQENYELGNEDEEKNERSSSDSEEKSNLEN--LLATQE 193

Query: 72  SHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTL----------- 131
           +++ YC +C + IT+ ++L+       ++++ +     P   PD  S +           
Sbjct: 194 NYELYCPSCSTCITRNVVLKKRKRGKHVNSSLDLKPDIPVVEPDEPSDIEEMESPVKVYV 253

Query: 132 -QPRYDTDSE-------SCCIS------------------------------VECRGNVY 191
            + R + D E       +C +                               ++ R ++ 
Sbjct: 254 PETRIEDDQEDKEGTIFTCLVCDLKYFIRLGTKFLQLDYIRGKPVEKSVEEYIDVRKSIN 313

Query: 192 MTFVSNRPEP---RRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMI 251
            T    + +P   R   E++KS VYGGLTE ITSLG+V+SA+++      I ALA+AN+ 
Sbjct: 314 TTQSPPQIQPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLA 373

Query: 252 IGLIVLTKNFSTLKSNQPMKYPYRRKRPQVDPYEEAFGDRQNYLLHYSAIILSFLLIGLL 311
            GLIVL +NF  L+++            + D YEE  G R    +H    ++S++  GL+
Sbjct: 374 GGLIVLAQNFQDLRNSSDQ---------EKDRYEELLGRRTKSRIHILVAVMSYIFFGLI 433

Query: 312 PPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHK------YVITLVCY 330
           PPLVY+FSF  T   N KL  V   SL+C+ LL   K YVRKP +       Y+ +   Y
Sbjct: 434 PPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYY 493

BLAST of CmoCh08G002350 vs. TAIR 10
Match: AT5G24290.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 120.6 bits (301), Expect = 2.5e-27
Identity = 107/385 (27.79%), Postives = 176/385 (45.71%), Query Frame = 0

Query: 12  ETENQPTPAAGESKSRAMKLSRTSSS---------DSDEVYSDSDTDTDVENQRLYELPN 71
           E  N+ + +  E KS   KL  T  +          ++   SDS+  +++EN  L     
Sbjct: 150 EENNERSLSDSEEKSNLEKLLGTQENYELGNEDEEKNERSSSDSEEKSNLEN--LLATQE 209

Query: 72  SHDFYCANCESSITKVIILRNPPSRSLISAARESTEPPPSCSPDLMSTL----------- 131
           +++ YC +C + IT+ ++L+       ++++ +     P   PD  S +           
Sbjct: 210 NYELYCPSCSTCITRNVVLKKRKRGKHVNSSLDLKPDIPVVEPDEPSDIEEMESPVKVYV 269

Query: 132 -QPRYDTDSE-------SCCIS------------------------------VECRGNVY 191
            + R + D E       +C +                               ++ R ++ 
Sbjct: 270 PETRIEDDQEDKEGTIFTCLVCDLKYFIRLGTKFLQLDYIRGKPVEKSVEEYIDVRKSIN 329

Query: 192 MTFVSNRPEP---RRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMI 251
            T    + +P   R   E++KS VYGGLTE ITSLG+V+SA+++      I ALA+AN+ 
Sbjct: 330 TTQSPPQIQPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLA 389

Query: 252 IGLIVLTKNFSTLKSNQPMKYPYRRKRPQVDPYEEAFGDRQNYLLHYSAIILSFLLIGLL 311
            GLIVL +NF  L+++            + D YEE  G R    +H    ++S++  GL+
Sbjct: 390 GGLIVLAQNFQDLRNSSDQ---------EKDRYEELLGRRTKSRIHILVAVMSYIFFGLI 449

Query: 312 PPLVYSFSFRHTDDNNLKLAVVGAISLLCIALLAIAKAYVRKPLHK------YVITLVCY 330
           PPLVY+FSF  T   N KL  V   SL+C+ LL   K YVRKP +       Y+ +   Y
Sbjct: 450 PPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYY 509

BLAST of CmoCh08G002350 vs. TAIR 10
Match: AT4G27860.1 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 113.6 bits (283), Expect = 3.0e-25
Identity = 106/321 (33.02%), Postives = 151/321 (47.04%), Query Frame = 0

Query: 19  PAAGESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYELPNSHDFYCANCESSITKV 78
           P++ E  S+      T + D +    D D D + ENQ+ +  P     Y +  E      
Sbjct: 324 PSSLEKPSKQTVNKETQNHDKEAADPDQDVDKETENQKSHLTP----IYPSPLEQ----- 383

Query: 79  IILRNPPSRSLISAARESTEP--PPSCSPDLMSTLQPRYDTDSESCCISVECRGNVYMTF 138
                 PS+ +I+   + TEP  PP+   ++ ++++PR               GN     
Sbjct: 384 ------PSKQIINKETQ-TEPMLPPNAQSEIPNSVEPRKG-------------GNK---- 443

Query: 139 VSNRPEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMIIGLIVL 198
                      EI+KSIVYGGLTE+ITSL  VTSAA++      + AL +AN+  GL++ 
Sbjct: 444 ----------VEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLT 503

Query: 199 TKNFSTLKSNQPMKYPYRRKRP-----QVDPYEEAFGDRQNYLLHYSAIILSFLLIGLLP 258
             +   L + +P K       P     + D YEE  G R+   +H    I SF++ GL+P
Sbjct: 504 VHSLQELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIP 563

Query: 259 PLVYSFSFRHTDD--NNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYVITLVCYIVLGL 318
           PLVY FSFR   +     K+  V A+SLLCI LL+IAKAYV K    YV TL  Y     
Sbjct: 564 PLVYGFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKK-RDYVKTLFRYTTTAT 600

Query: 319 GVFGLSYLGGKVLGKLLEPLG 331
              G S   G ++ + LE  G
Sbjct: 624 TASGFSQFVGYLVSQWLEKSG 600

BLAST of CmoCh08G002350 vs. TAIR 10
Match: AT4G27860.2 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 112.1 bits (279), Expect = 8.9e-25
Identity = 105/317 (33.12%), Postives = 148/317 (46.69%), Query Frame = 0

Query: 23  ESKSRAMKLSRTSSSDSDEVYSDSDTDTDVENQRLYELPNSHDFYCANCESSITKVIILR 82
           E  S+      T + D +    D D D + ENQ+ +  P     Y +  E          
Sbjct: 313 EKPSKQTVNKETQNHDKEAADPDQDVDKETENQKSHLTP----IYPSPLEQ--------- 372

Query: 83  NPPSRSLISAARESTEP--PPSCSPDLMSTLQPRYDTDSESCCISVECRGNVYMTFVSNR 142
             PS+ +I+   + TEP  PP+   ++ ++++PR               GN         
Sbjct: 373 --PSKQIINKETQ-TEPMLPPNAQSEIPNSVEPRKG-------------GNK-------- 432

Query: 143 PEPRRCFEIVKSIVYGGLTEAITSLGIVTSAASANIPIEIIGALALANMIIGLIVLTKNF 202
                  EI+KSIVYGGLTE+ITSL  VTSAA++      + AL +AN+  GL++   + 
Sbjct: 433 ------VEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVHSL 492

Query: 203 STLKSNQPMKYPYRRKRP-----QVDPYEEAFGDRQNYLLHYSAIILSFLLIGLLPPLVY 262
             L + +P K       P     + D YEE  G R+   +H    I SF++ GL+PPLVY
Sbjct: 493 QELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPLVY 552

Query: 263 SFSFRHTDD--NNLKLAVVGAISLLCIALLAIAKAYVRKPLHKYVITLVCYIVLGLGVFG 322
            FSFR   +     K+  V A+SLLCI LL+IAKAYV K    YV TL  Y        G
Sbjct: 553 GFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKK-RDYVKTLFRYTTTATTASG 585

Query: 323 LSYLGGKVLGKLLEPLG 331
            S   G ++ + LE  G
Sbjct: 613 FSQFVGYLVSQWLEKSG 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LPT31.5e-2941.88Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL... [more]
F4KFS73.5e-2627.79Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1[more]
Q8W4P84.3e-2433.02Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H7C47.0e-17996.28membrane protein of ER body-like protein OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1KVU91.1e-8387.18membrane protein of ER body 2-like OS=Cucurbita maxima OX=3661 GN=LOC111498065 P... [more]
A0A6J1GPB81.6e-5846.15membrane protein of ER body 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1GPB53.6e-5845.76membrane protein of ER body 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JXX61.1e-5746.11membrane protein of ER body-like protein isoform X2 OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
XP_022960407.11.5e-17896.28membrane protein of ER body-like protein [Cucurbita moschata][more]
KAG6593019.18.9e-15287.57Membrane protein of ER body 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023514589.11.7e-14786.75membrane protein of ER body-like protein [Cucurbita pepo subsp. pepo][more]
XP_023004885.12.3e-8387.18membrane protein of ER body 2-like [Cucurbita maxima][more]
KAG7025428.16.9e-8055.92Membrane protein of ER body-like protein, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
AT4G27870.11.1e-3041.88Vacuolar iron transporter (VIT) family protein [more]
AT5G24290.22.5e-2727.79Vacuolar iron transporter (VIT) family protein [more]
AT5G24290.12.5e-2727.79Vacuolar iron transporter (VIT) family protein [more]
AT4G27860.13.0e-2533.02vacuolar iron transporter (VIT) family protein [more]
AT4G27860.28.9e-2533.12vacuolar iron transporter (VIT) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..50
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..41
NoneNo IPR availablePANTHERPTHR31851:SF64GLYCINE-RICH PROTEIN FAMILYcoord: 138..330
NoneNo IPR availablePANTHERPTHR31851:SF64GLYCINE-RICH PROTEIN FAMILYcoord: 25..122
IPR008217Ccc1 familyPANTHERPTHR31851FE(2+)/MN(2+) TRANSPORTER PCL1coord: 25..122
IPR008217Ccc1 familyPANTHERPTHR31851FE(2+)/MN(2+) TRANSPORTER PCL1coord: 138..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G002350.1CmoCh08G002350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030026 cellular manganese ion homeostasis
biological_process GO:0071421 manganese ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005384 manganese ion transmembrane transporter activity