CmoCh08G001500 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G001500
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionautophagy-related protein 3-like
LocationCmo_Chr08: 862808 .. 869441 (-)
RNA-Seq ExpressionCmoCh08G001500
SyntenyCmoCh08G001500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCCCCCCCCAGAGAAGCCCAAATTCGCTGGTTGATCAGAGTCGAGCTTCCTCTGGGGATTCCAAATTCCTAGTCCAATTTCCAAAATCTTTCATCTGAATTCATCAATTTCAGAGGCATTGAGATCATGGTTTTGTCGCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTCGAGAGGATCACAGGGCCTCGAACCGTTTCCGCCTTCAAAGAGAAAGGCGTTCTCAGTGTCTCTGAGTTCATCGTCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGTATTTCCATGTGAATTCTCTCCATTTTCCTGATCGAAGTTGTTTTATATATTTGTTGAATTTGTTCTTTTGCGATATTCGAAATTGATAGACGTGTTCTTCAACTGATGAATTGAACGGATGTCTCTATTAGAAGATTCCGTGCATTCATCAATTATATCAATTCTCTATCCCGAATCCTGTAATTTTTGTCAATTCTGTTTTCCAAGAGACTGAAGTCGTTTATGGCTGTTCGAACGAAACCAAGGTTAATTGGTCTAGGAGGTGAGGTCTGTGCCGGTGTCAAAGCCTTTTATTGTAAACTTCGATGAAGGAGTTTGGTTATCTCCGTTTTAGTGAATTGGAAGGGTTGGGAAAAAAATCTTTAATGATTTGATTTAAAATGCCTTCTGAATCAAAGGTTCTTGAGAAAGCTAAAGAGGAGAGTGGTACATATTGTGTGTAGAAAGGACATTAATAATAGTGATATCCCATTAGTGTTTCTTATTTTTCCTGAAACCATGACTACGACCTTCGTTTGACTTAAGTTTGTTATTTGTTTTTGAATTATAAAATTTAGTGGAACAAAATTAGAGTGGTATCCATTGCTTCTGTATGCGTACTTAGTTTTAGGTTCTTCATGGTTCGGAAGGGCAGTAATAACCCAAATGTCGACTATCCAGTGTATGTTATACACCGTCCTGTCCCTTTTTCAGGCGAAAGACAATGTGGATCTTTCCACAATTTAAAAAACTGACATTATTGGTGGTTCACTTTTACAGTTGCACTCTTAATTTTCTCACCTCCTGTATTTGGAATTAAAATTTGGAAATGTTTTGAATTTATCTAATACATGTTAAAATATGTTTTCCTTTTTCATGATTTTAGGGAGTCAGGTGATCTGAATAAGAGGAAATCATACTTGCCCCCAGATAAGCAATTCTTAATTACCAGAAATGGTATAACTGCATAATCATTAACTTTTTTTCTTTTCTTTTTTTTGTAATTCGAGGGGTTACAGTCAGAACCGTAATGTTTTGTAATTTTTCTGTCCATACCTTGTAGTTTATGTGCTTTTGACACTTACACTTCTCCTTCCCATAATCTTCTATTAATTTATGGTCATTTATCTGAAATATTGTTATGAACAAAAAGAATTTTGCATGTTATCAGGCCATTTTTCTAAACCATACTCTTTCTACTCATGCCTTAAAATTATGTCGCTCTCCACAATGAGACTTACACTTGGCAGGGAACCTAATCAATAGAAACAACTCACGTTTCAATACCAAACATGAATTACTATTTTCTGCAACATATAGTGCACCTTTGAGACACGGAGTTCAAGTTTGTATGTATATCTTTTCTAGAAATTAGTATTTAACCTGTAGCTAATTGCCAAATTAATTCTTTGGTAACTGTTTCAGCACCGGTAAGGTCAATTATATACTTTTTGCAAATAATGGTTTGGCGTTATGTCAAATATTGAATGAATTCTTTGGCATATCTAGACTTCAACTTTATTGAGCCATTATCAAAAGTAAGGATGATTGTTCACTTTCTTTCCAGTTCCTTGCCTTCGCCGGGCTGCCTATGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTTTACTTGATGAGGAAGATGGCGATGGCTGGCTGGCAACTCATGGCAAACCGAAGGGTACCTTTATCTTTCAGTGTAAAGCTAGGGATTTACATTTGTTTAATAACATGTGTTCTTTCATGCAGAAGAAAAAAGCGATGAAGAGGAAAATTTGCCTTCCCTGGAAACTCTTGAAATTAGCAGGACCAAGATTGTTAAGTCAATTCCAAACTATTTTGGTGGTGAAGATGAAGATGGTATTCCTGACCTTGCTGATATTGAAGAAAATGATGACGCTCTTGCAACTGATGCAGTATGTTAATTTTTATGATCCAATTTATGAGCAAATGTGGCTAAAAAATACTCAACTTTATAGGAGGGCCTACATAACCACCGCCCTCCCACCCCCATCCCCATGATCTCTTAATTTGCAAGGCATGGATTGTGGAAAATAATCTAGAATCTTTCTTGGCATCTCAACTTGTAAAAGTTTTCTTGAGTCTGATGACGAAAGTTCTTGCTTCATTATCATCATGTACACATTTACCATTTGGGATTAAAAACTCTTTATGATCATCCTGCATTGTTGTGATTTTGATAAGGTATTCATGTTCACATTTTCATTGTAGGCCACTCTGCCATTGACCTACCAAATTGCCCATGAACCTGATGACGATAATATCCTTCGAACACGAACTTATGATGTCAGCATCACGTAAGGCGGTCTCTTCTTTCTTGTTTTGTCTTTTTCGTAATTCTTCCAAAGATTCTTATAATGTATCTGTTGCATAAAAATGGATCAAAATTCTTGGGTTGGTGAAAAAAATCCTTCTTTCTAGCCAAAGAGGGAAGTTATGGCAAGAAAAATTGATGCAATTGGAGTAAATAGCATAGTAAGAGGGTAGTTAGAAATATCTCAGAAATGATCCCTATATGAAGCTTGAATTCAATTTGTGTTCTTCCATAATTATTTTCCTGTCTATAAAATTCTCCACTTTCTTCTACTCCAAATCTCCGAAGCACTGCAAAGACTGCCATTGGAAAGAGTTGTAACGGATTTGGATCAGATAGCCCAGATGCGGTTATTACTTTACGTTTGGTTTTCTTAGGCAGTCATTGTTATTGTTAATCACTTTGAAAATAATTATTAGCAAGTTCATTTGGAGCATTGGTGCACTACTTGGGGATTCAAATCTTTGCCATTTTTGTCAAGTAGGAAATTATCACTGTCGGACTTGGGATTTTTTCAGGGTTGGGCATATAGGTAATGTTCGTGGCTTCAACTTTAGTGGTCCTAGGTTTAAATCTTAAAAAATAGACATACTGGTTGATGATCTTGGCCTCAATGTGAGTGGTTCTGGCTTCTTGGCCCCGAAAACATTCTTGTGGTAAAAGGCTTAGGCTCAAAATGACAAAGGTGGATAAAAGGATGCATCTCATTTACAAACTTCTCTATCATCATCAATGGAAGACCGAGAGGTAAAATTTATGCCACATGGGGTCCCCGACAAGGAGATCGACTATCCCCTTTTCTTTTCATCATAGTAATGTATTGCCAGATAAAATGTTTTCAGATTGGTAATGAAGGCTTAAGCATCAACCACCTTCAATTCCGGACGACACAATACTCTTTTCTGACTTGGCAGATGCCTCTTCCATAAAAAACATGATCAAGATGGTAAAAACCTTTGAAGGATTCTCTGGACAAAATATCAATCTCCAAAAAAACAAAAAGCATGGTCATCAATATTAGCACAGAGATTATTGAAGAATTTTCTAGCATATATGGTTGTATAAAGGGAGAATGATCGAATATGTACCTAGGACTACCTCTAAAAGGAAACCACGAATCTTTCTCCTTATGGGAGGTTATTATTGAAAAAATTGAAAGAAGGCTTTCAACATGGTCATCATGTTATACTTCAAAAGGTGGAAGACTCACCTTAATACAAGCTACATTATCAAACCTCCCCACTTACTGCGTGTCTCTATTTGAAATGTCCATCTACACCTTGTTCGATGGAATATTATAAACCTCCCAATAGAAAAGGGAGATCTCGATCTTTTCTCGATAAAGAAGAAGAACAAAGCCCTCATCGCCAAATGGATATGGAGATATTATCATGAAGAAAAGGCCTTGTGGAGAAATCTTATAAAGGCCAAATATACTCTTACTTCAAACAAAAATCAACCCCTCCATCTTCTACAAAAGGGCCTTGGAAGTACGTAAGGAAACATCAAAACCTCATCACCAACTAAACTTGCCATAGGGTGGGTGATGGAGGAAGCACATCATTGTGGACCGACCCCTGGATTGAAAACACCATGCTAGCCTTGAGGTATCAACTTTTGTACAGATTCTCACATAGCAAAACAACAAGGATCAAAGAAACGTGGAATGTTGTCAACAAATTTTGGGACCTAAAGGGAATTATAATGAAGCAACGGAGTGTGCTGAATTAAGCCTTGACCTTGCCCCCGTGGTATTGTCAAATAAAGAAGACTCACTAACTTAGCTCCCTAGCACTGATAGGGTTCTCTACAAAATCCTTGATGATGGACATGGGGGAAAAAGTAGAAGCAATAAATACCATGCTAGCAAAGATAGTATGGAAAGGACATCATCGTAAAAAGATGAATTTTTTTCTTTGGAAAATAGCGCATAAAGCCATTACCACAAGTGAAAATCTTCGGAAAAAAAAGAAAAAAAAGGCTTATATCTCTCTTTCTCCAAATTGGTGTCCATTATGCGAGAAAGGAAGTGAATCACATAGCCACTTATTTATGCAATCACGTACGCTCTGAATTTCTGGACAACGATTCTCAATATATTCGAATGGCATCTCACATTTCTAGGGAGGTAAAGAGTCTATTGGACATGACCTTAATGTACCACCCTTTCAAGAATGCAAAAGCCCTACTATGAGAAAACCTCATCATGACTTTCTTTTGGAATCTGTGGAAAGAAAGAAATCAGAGAATATTTGCAGAAAAGACAAGACTTATACAAAACTTTTCGACAATGTTGTTTACCAAGCTATATCTTGGTGCAAACTGTCTAATATTTTTAATTCCTATTGGGAAGGTCTTTTGTAGACATCATGGATACGTCCCTTTTGTAAATTTCAATCTTCAGTGAAATTGTATCCTATGTAAAGAAAAAAAAAATGCAAGGGAATAACCATAGAAAAGGAATATGCCAAAAATATATCTGCAATAGCAATCCTATAAGACCATCTACTCTTTGGGACAAAGTGCCTATCATTATTGAATCATATCGTTTGTATGACGGGACTAAACTTGTAATTTGATCAAATTTTTTTCTATCTGATGATGCTATTTTTCTCTTATATCAGGTATGATAAATATTATCAGACACCACGTGTTTGGCTGACAGGATATGATGAGGTTTTGTATTTTTATTCTGTTTCCATTTCTAGTTTGGTAGAACTTTTGATCTTGTACATATAATCACCATGCATGCATTCTATTGCTATGCAAGTTCTTTTTCTTACACAACTTCTATTAGCATGCACGTATGTACTTAAATCAAGTATCTGTGAACTATGTCAGTCAAGGATGCTCTTACAGCCAGAACATGTGCTTGAAGATGTTAGTCAAGATCATGCACGAAAAACGGTAAGTTGTGATTATGTAATAATGTAGTTGTGCCATCTGCAAGAAATAAATTGATCCTGATATTAGGCCCATCATTTATTCCATGTTATTTTTGTTGCAAGTCTAAATTAGCTTGCTGTTTTTCTTTTCCTTTTGAGAGAGGTACATTTACCTTTTATACTATAAAAAAAGGTTAAATCTATGAATTATGAATTATCAAATTTGGATCCTTGTGGATGCGATAATCTATAATTTTACACATTATTAAGATGACCAGCAAGAATCCCTCTTGATGCATCCTGAACTCCTATTGTGGTGTCTGGAGGACCCATGCATTTGTTTTATTGATTTGCCCTTCCAACGGTCATGTGGAACATGATTTTCCAATTATGTTATAAGTTCAATCTTTGGACACAGGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCATCTGTACATCCCTGCAGACATGGGGCTGTGATGAAAAAGATCATTGATGTGTTGATGTCCCGGGGCGTAGAACCGGAAGTTCACAAGTAAGTATTATGAACTCCTATTTAAAACAGCAGATCTAGTTTTGGTGACAATCTTTGCTTGTTCCTAACCAAGTATCTCAAGCTCCTCTACTTATTAATTGTTTTCTTCTATCTTTTGTAGGTATCTTTTCTTGTTCTTAAAATTTGTCGCGTCGGTTATTCCGACTATCGAATATGATTATACTATGGACGTTGATCTTGGAGGCCCTAGCCATCGATAACTGGGATTGATGAATATTGTAAGCACAACAGGATCGTAATTCCTTTCAACTTCCGGCATCTTAACATGCTTGTGTACATCCAAAGTGCTGCTTATTATTACTTCTAGTGTTTATTTGTTGGAGTTTGGTTGTGGGTCAAGTAAAACGTACTATTTCCATTTTCTCAACAAAAGTGTGATTTCTTGCCAAAAGAAATAGGTCCATTGCTTGAGAAAATTCATGCACTACTCAATCAGTCGTACAATATACACTTAAATTTTTCTTTCAGAATACGTGGGTTTTAAAGGCTCAACTGTTTGCACTCCTTAACTATATATGCTTGCTAATGATGTAGTCCATTCGAAAAGTTTTAAAATCAGAGATATTATGGCGTG

mRNA sequence

CCCCCCCCCCCAGAGAAGCCCAAATTCGCTGGTTGATCAGAGTCGAGCTTCCTCTGGGGATTCCAAATTCCTAGTCCAATTTCCAAAATCTTTCATCTGAATTCATCAATTTCAGAGGCATTGAGATCATGGTTTTGTCGCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTCGAGAGGATCACAGGGCCTCGAACCGTTTCCGCCTTCAAAGAGAAAGGCGTTCTCAGTGTCTCTGAGTTCATCGTCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGTTCTTCATGGTTCGGAAGGGCAGTAATAACCCAAATGTCGACTATCCAGTGGAGTCAGGTGATCTGAATAAGAGGAAATCATACTTGCCCCCAGATAAGCAATTCTTAATTACCAGAAATGTTCCTTGCCTTCGCCGGGCTGCCTATGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTTTACTTGATGAGGAAGATGGCGATGGCTGGCTGGCAACTCATGGCAAACCGAAGGAAGAAAAAAGCGATGAAGAGGAAAATTTGCCTTCCCTGGAAACTCTTGAAATTAGCAGGACCAAGATTGTTAAGTCAATTCCAAACTATTTTGGTGGTGAAGATGAAGATGGTATTCCTGACCTTGCTGATATTGAAGAAAATGATGACGCTCTTGCAACTGATGCAGCCACTCTGCCATTGACCTACCAAATTGCCCATGAACCTGATGACGATAATATCCTTCGAACACGAACTTATGATGTCAGCATCACGTATGATAAATATTATCAGACACCACGTGTTTGGCTGACAGGATATGATGAGTCAAGGATGCTCTTACAGCCAGAACATGTGCTTGAAGATGTTAGTCAAGATCATGCACGAAAAACGGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCATCTGTACATCCCTGCAGACATGGGGCTGTGATGAAAAAGATCATTGATGTGTTGATGTCCCGGGGCGTAGAACCGGAAGTTCACAAGTATCTTTTCTTGTTCTTAAAATTTGTCGCGTCGGTTATTCCGACTATCGAATATGATTATACTATGGACGTTGATCTTGGAGGCCCTAGCCATCGATAACTGGGATTGATGAATATTGTAAGCACAACAGGATCGTAATTCCTTTCAACTTCCGGCATCTTAACATGCTTGTGTACATCCAAAGTGCTGCTTATTATTACTTCTAGTGTTTATTTGTTGGAGTTTGGTTGTGGGTCAAGTAAAACGTACTATTTCCATTTTCTCAACAAAAGTGTGATTTCTTGCCAAAAGAAATAGGTCCATTGCTTGAGAAAATTCATGCACTACTCAATCAGTCGTACAATATACACTTAAATTTTTCTTTCAGAATACGTGGGTTTTAAAGGCTCAACTGTTTGCACTCCTTAACTATATATGCTTGCTAATGATGTAGTCCATTCGAAAAGTTTTAAAATCAGAGATATTATGGCGTG

Coding sequence (CDS)

ATGGTTTTGTCGCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTCGAGAGGATCACAGGGCCTCGAACCGTTTCCGCCTTCAAAGAGAAAGGCGTTCTCAGTGTCTCTGAGTTCATCGTCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGTTCTTCATGGTTCGGAAGGGCAGTAATAACCCAAATGTCGACTATCCAGTGGAGTCAGGTGATCTGAATAAGAGGAAATCATACTTGCCCCCAGATAAGCAATTCTTAATTACCAGAAATGTTCCTTGCCTTCGCCGGGCTGCCTATGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTTTACTTGATGAGGAAGATGGCGATGGCTGGCTGGCAACTCATGGCAAACCGAAGGAAGAAAAAAGCGATGAAGAGGAAAATTTGCCTTCCCTGGAAACTCTTGAAATTAGCAGGACCAAGATTGTTAAGTCAATTCCAAACTATTTTGGTGGTGAAGATGAAGATGGTATTCCTGACCTTGCTGATATTGAAGAAAATGATGACGCTCTTGCAACTGATGCAGCCACTCTGCCATTGACCTACCAAATTGCCCATGAACCTGATGACGATAATATCCTTCGAACACGAACTTATGATGTCAGCATCACGTATGATAAATATTATCAGACACCACGTGTTTGGCTGACAGGATATGATGAGTCAAGGATGCTCTTACAGCCAGAACATGTGCTTGAAGATGTTAGTCAAGATCATGCACGAAAAACGGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCATCTGTACATCCCTGCAGACATGGGGCTGTGATGAAAAAGATCATTGATGTGTTGATGTCCCGGGGCGTAGAACCGGAAGTTCACAAGTATCTTTTCTTGTTCTTAAAATTTGTCGCGTCGGTTATTCCGACTATCGAATATGATTATACTATGGACGTTGATCTTGGAGGCCCTAGCCATCGATAA

Protein sequence

MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRKGSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR
Homology
BLAST of CmoCh08G001500 vs. ExPASy Swiss-Prot
Match: Q0WWQ1 (Autophagy-related protein 3 OS=Arabidopsis thaliana OX=3702 GN=ATG3 PE=1 SV=2)

HSP 1 Score: 521.9 bits (1343), Expect = 5.2e-147
Identity = 256/330 (77.58%), Postives = 282/330 (85.45%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEF++AGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFVLAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGD +KRK YLP DKQFLITRNVPCLRRAA V E+YEAAGGEVL+D+E
Sbjct: 61  -----------ESGDASKRKPYLPSDKQFLITRNVPCLRRAASVAEDYEAAGGEVLVDDE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           D DGWLATHGKPK +K  EE+NLPS++ L+I+    ++SIP YFGGE++D IPD+ + +E
Sbjct: 121 DNDGWLATHGKPK-DKGKEEDNLPSMDALDINEKNTIQSIPTYFGGEEDDDIPDMEEFDE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
            D+ +  D ATL  TY +AHEPDDDNILRTRTYD+SITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 ADNVVENDPATLQSTYLVAHEPDDDNILRTRTYDLSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPE V+EDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEV K
Sbjct: 241 QPELVMEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVDK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPS 331
           YLFLFLKF+ASVIPTIEYDYTMD DLG  S
Sbjct: 301 YLFLFLKFMASVIPTIEYDYTMDFDLGSSS 312

BLAST of CmoCh08G001500 vs. ExPASy Swiss-Prot
Match: Q550A8 (Autophagy-related protein 3 OS=Dictyostelium discoideum OX=44689 GN=atg3 PE=3 SV=3)

HSP 1 Score: 275.8 bits (704), Expect = 6.5e-73
Identity = 159/350 (45.43%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 5   QKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRKGSNN 64
           Q +H+A+  TVE++T   + S F E+GVL+  EF+ AGD L  KC TW+W          
Sbjct: 7   QAVHKAYVKTVEKVTPTLSTSKFLEEGVLTPEEFVQAGDLLTDKCQTWTW---------- 66

Query: 65  PNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDG-D 124
                  ESGD ++  SYLP +KQFL+TRNVPC  R   +E E +A+  + +  E+DG D
Sbjct: 67  -------ESGDPSRNVSYLPKEKQFLLTRNVPCYNRVRTLENESKASKADEIQIEDDGED 126

Query: 125 GWLATHGKPKEEKSDEEENLPSLETLEI-------SRTKIVKSIPNYFGG-----EDEDG 184
            W+A   +P   + D E+    + +L+I       + T   K   N         EDEDG
Sbjct: 127 SWVAP--QPVGNQDDIEDEKVDISSLKIDDKKPNTTTTTTAKPTNNNNNNNDDEDEDEDG 186

Query: 185 -IPDLAD------IEENDDALATDAATLPLTYQIAHE---------PDDDNILRTRTYDV 244
            IPDL D      IEE D A+ +       T    +           D+DNILRTRTYD+
Sbjct: 187 DIPDLDDFQDDNIIEEEDPAVLSKNNKTTTTTTANNNNNNNSENKVEDNDNILRTRTYDI 246

Query: 245 SITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHP 304
           SITYDKYYQTPRVWL GYDE+R  L+PE + ED+S+DHA KTVTI+ HPHL    A +HP
Sbjct: 247 SITYDKYYQTPRVWLFGYDENRKPLKPEEIFEDISEDHAHKTVTIDSHPHLGISFAYIHP 306

Query: 305 CRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYDYTMDVD 326
           CRH AVMKK++D     G EP V +YLFLFLKF++ VIPTIEYD+T++ D
Sbjct: 307 CRHAAVMKKLVDRQSENGKEPRVDQYLFLFLKFISVVIPTIEYDFTLEFD 337

BLAST of CmoCh08G001500 vs. ExPASy Swiss-Prot
Match: Q9CPX6 (Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 9.3e-64
Identity = 143/314 (45.54%), Postives = 185/314 (58.92%), Query Frame = 0

Query: 16  ERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRKGSNNPNVDYPVESGD 75
           E +T     S FKE GV++  EF+ AGD+LV  CPTW W                  +G+
Sbjct: 17  EYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQW-----------------ATGE 76

Query: 76  LNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGD-GWLATHGKPKE 135
             K K+YLP DKQFL+T+NVPC +R   +E   E    E +++E+DGD GW+ T+     
Sbjct: 77  ELKVKAYLPTDKQFLVTKNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTYHNTGI 136

Query: 136 EKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPL 195
               E     +LE+ +  + +   ++ +    EDE    D+ + EE+   L TD ATL  
Sbjct: 137 TGITEAVKEITLESKDSIKLQDCSALCDEEDEEDEGEAADMEEYEES-GLLETDEATLDT 196

Query: 196 TYQI------AHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLED 255
              +      A    +D IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  EH+ ED
Sbjct: 197 RKIVEACKAKADAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYED 256

Query: 256 VSQDHARKTVTIEDHPHL-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLK 315
           +SQDH +KTVTIE+HPHL P    SVHPCRH  VMKKII+ +   G E  VH YL +FLK
Sbjct: 257 ISQDHVKKTVTIENHPHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLK 309

Query: 316 FVASVIPTIEYDYT 322
           FV +VIPTIEYDYT
Sbjct: 317 FVQAVIPTIEYDYT 309

BLAST of CmoCh08G001500 vs. ExPASy Swiss-Prot
Match: Q6PFS7 (Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-63
Identity = 145/319 (45.45%), Postives = 184/319 (57.68%), Query Frame = 0

Query: 13  GTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRKGSNNPNVDYPVE 72
           G  E +T     S FKE GV++  EF+ AGD+LV  CPTW W                  
Sbjct: 14  GVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKW-----------------A 73

Query: 73  SGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGD-GWLATHGK 132
           SG+  K K YLP DKQFL+TRNVPC +R   +E   E    E +++E+DGD GW+ T   
Sbjct: 74  SGEEAKVKPYLPNDKQFLLTRNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTFHN 133

Query: 133 PKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDA--LATDA 192
                  E     SL+  +     +        G +D+D   + AD+EE +++  L TD 
Sbjct: 134 SGVTGVTEAVREISLDNKDNMNMNVKTGACGNSGDDDDDEEGEAADMEEYEESGLLETDD 193

Query: 193 ATL------PLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPE 252
           ATL       L+   A    +D IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  +
Sbjct: 194 ATLDTSKMADLSKTKAEAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEDRQPLTVD 253

Query: 253 HVLEDVSQDHARKTVTIEDHPHL-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYL 312
            + ED+SQDH +KTVTIE+HP+L P    SVHPCRH  VMKKII+ +   G E  VH YL
Sbjct: 254 QMYEDISQDHVKKTVTIENHPNLPPPAMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYL 312

Query: 313 FLFLKFVASVIPTIEYDYT 322
            +FLKFV +VIPTIEYDYT
Sbjct: 314 LIFLKFVQAVIPTIEYDYT 312

BLAST of CmoCh08G001500 vs. ExPASy Swiss-Prot
Match: Q6AZ50 (Ubiquitin-like-conjugating enzyme ATG3 OS=Rattus norvegicus OX=10116 GN=Atg3 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-62
Identity = 143/319 (44.83%), Postives = 191/319 (59.87%), Query Frame = 0

Query: 16  ERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRKGSNNPNVDYPVESGD 75
           E +T     S FKE GV++  EF+ AGD+LV  CPTW W                  +G+
Sbjct: 17  EYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQW-----------------ATGE 76

Query: 76  LNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGD-GWLATH---GK 135
             K K+YLP  KQFL+T+NVPC +R   +E   E    E +++E+DGD GW+ T+   G 
Sbjct: 77  ELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTYHNTGI 136

Query: 136 PKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDA--LATDA 195
               ++ +E  L S +++++    ++         E+E+   + AD+EE +++  L TD 
Sbjct: 137 TGITEAVKEITLESKDSIKLQDCSVL------CDEEEEEEEGEAADMEEYEESGLLETDE 196

Query: 196 ATLPLTYQI------AHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPE 255
           ATL     +      A    +D IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  E
Sbjct: 197 ATLDTRRIVEACKAKADAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVE 256

Query: 256 HVLEDVSQDHARKTVTIEDHPHL-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYL 315
           H+ ED+SQDH +KTVTIE+HPHL P    SVHPCRH  VMKKII+ +   G E  VH YL
Sbjct: 257 HMYEDISQDHVKKTVTIENHPHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYL 309

Query: 316 FLFLKFVASVIPTIEYDYT 322
            +FLKFV +VIPTIEYDYT
Sbjct: 317 LIFLKFVQAVIPTIEYDYT 309

BLAST of CmoCh08G001500 vs. ExPASy TrEMBL
Match: A0A6J1H511 (autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460636 PE=4 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 5.9e-178
Identity = 315/332 (94.88%), Postives = 315/332 (94.88%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE
Sbjct: 61  -----------ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
           NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 333
           YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 315

BLAST of CmoCh08G001500 vs. ExPASy TrEMBL
Match: A0A6J1KTV3 (autophagy-related protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111498158 PE=4 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.5e-177
Identity = 313/332 (94.28%), Postives = 314/332 (94.58%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEFIVAGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFIVAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE
Sbjct: 61  -----------ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
           NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 333
           YLFLFLKFVASVIPTIEYDYTMDVDLGGPS R
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSRR 315

BLAST of CmoCh08G001500 vs. ExPASy TrEMBL
Match: A0A1S3CAI3 (autophagy-related protein 3 OS=Cucumis melo OX=3656 GN=LOC103498815 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.4e-168
Identity = 297/330 (90.00%), Postives = 303/330 (91.82%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFI+AGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGDLNKRKSYLPPDKQFLITRNVPCLRRAA +EEEYEAAGGEVLLD E
Sbjct: 61  -----------ESGDLNKRKSYLPPDKQFLITRNVPCLRRAASIEEEYEAAGGEVLLDNE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLE LEISRTK+VKSIPNYFGGEDED +PDL DIEE
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
            D+ + TDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 TDNVVGTDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPE VLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPELVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPS 331
           YLFLFLKFVASVIPTIEYDYTMD DLGGPS
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDFDLGGPS 313

BLAST of CmoCh08G001500 vs. ExPASy TrEMBL
Match: A0A6J1H4I9 (autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460417 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.4e-168
Identity = 297/332 (89.46%), Postives = 304/332 (91.57%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           M LSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFI+AGDNLVSKCPTWSW      
Sbjct: 1   MALSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGD+NKRKSYLPPDKQFLITRNVPCLRRAA VEEEYEAAGGEVLLD E
Sbjct: 61  -----------ESGDINKRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLETLEISR K+VKSIPNYFGGEDE+ IPDLADIE+
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRPKVVKSIPNYFGGEDEEDIPDLADIED 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
            D+ + TDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 TDNVIGTDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPE VLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPELVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 333
           YLFLFLKFVASVIPTIEYDYTMD DLGGPS R
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDFDLGGPSSR 315

BLAST of CmoCh08G001500 vs. ExPASy TrEMBL
Match: A0A5A7T8K5 (Autophagy-related protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G00500 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.4e-168
Identity = 297/330 (90.00%), Postives = 303/330 (91.82%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFI+AGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGDLNKRKSYLPPDKQFLITRNVPCLRRAA +EEEYEAAGGEVLLD E
Sbjct: 61  -----------ESGDLNKRKSYLPPDKQFLITRNVPCLRRAASIEEEYEAAGGEVLLDNE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLE LEISRTK+VKSIPNYFGGEDED +PDL DIEE
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
            D+ + TDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 TDNVVGTDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPE VLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPELVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPS 331
           YLFLFLKFVASVIPTIEYDYTMD DLGGPS
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDFDLGGPS 313

BLAST of CmoCh08G001500 vs. NCBI nr
Match: XP_022959612.1 (autophagy-related protein 3-like [Cucurbita moschata])

HSP 1 Score: 633.3 bits (1632), Expect = 1.2e-177
Identity = 315/332 (94.88%), Postives = 315/332 (94.88%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE
Sbjct: 61  -----------ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
           NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 333
           YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 315

BLAST of CmoCh08G001500 vs. NCBI nr
Match: KAG7025347.1 (Autophagy-related protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 630.2 bits (1624), Expect = 1.0e-176
Identity = 314/332 (94.58%), Postives = 314/332 (94.58%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE
Sbjct: 61  -----------ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
           NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 333
           YLFLFLKFVASVIPTIEYDYTMDVDLGGPS R
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSRR 315

BLAST of CmoCh08G001500 vs. NCBI nr
Match: XP_023005051.1 (autophagy-related protein 3-like [Cucurbita maxima])

HSP 1 Score: 629.8 bits (1623), Expect = 1.3e-176
Identity = 313/332 (94.28%), Postives = 314/332 (94.58%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEFIVAGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFIVAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE
Sbjct: 61  -----------ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
           NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 333
           YLFLFLKFVASVIPTIEYDYTMDVDLGGPS R
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSRR 315

BLAST of CmoCh08G001500 vs. NCBI nr
Match: XP_023514388.1 (autophagy-related protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629.0 bits (1621), Expect = 2.3e-176
Identity = 313/332 (94.28%), Postives = 314/332 (94.58%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE
Sbjct: 61  -----------ESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADI+E
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIDE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
           NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 333
           YLFLFLKFVASVIPTIEYDYTMDVDLGGPS R
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSRR 315

BLAST of CmoCh08G001500 vs. NCBI nr
Match: KAG7013823.1 (Autophagy-related protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 600.1 bits (1546), Expect = 1.1e-167
Identity = 297/332 (89.46%), Postives = 304/332 (91.57%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           M LSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFI+AGDNLVSKCPTWSW      
Sbjct: 1   MALSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGD+NKRKSYLPPDKQFLITRNVPCLRRAA VEEEYEAAGGEVLLD E
Sbjct: 61  -----------ESGDINKRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           DGDGWLATHGKPKEEKSDEEENLPSLETLEISR K+VKSIPNYFGGEDE+ IPDLADIE+
Sbjct: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRPKVVKSIPNYFGGEDEEDIPDLADIED 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
            D+ + TDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 TDNVIGTDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPE VLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK
Sbjct: 241 QPEFVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPSHR 333
           YLFLFLKFVASVIPTIEYDYTMD DLGGPS R
Sbjct: 301 YLFLFLKFVASVIPTIEYDYTMDFDLGGPSSR 315

BLAST of CmoCh08G001500 vs. TAIR 10
Match: AT5G61500.1 (autophagy 3 (APG3) )

HSP 1 Score: 521.9 bits (1343), Expect = 3.7e-148
Identity = 256/330 (77.58%), Postives = 282/330 (85.45%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWFFMVRK 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEF++AGDNLVSKCPTWSW      
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFVLAGDNLVSKCPTWSW------ 60

Query: 61  GSNNPNVDYPVESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEE 120
                      ESGD +KRK YLP DKQFLITRNVPCLRRAA V E+YEAAGGEVL+D+E
Sbjct: 61  -----------ESGDASKRKPYLPSDKQFLITRNVPCLRRAASVAEDYEAAGGEVLVDDE 120

Query: 121 DGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEE 180
           D DGWLATHGKPK +K  EE+NLPS++ L+I+    ++SIP YFGGE++D IPD+ + +E
Sbjct: 121 DNDGWLATHGKPK-DKGKEEDNLPSMDALDINEKNTIQSIPTYFGGEEDDDIPDMEEFDE 180

Query: 181 NDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLL 240
            D+ +  D ATL  TY +AHEPDDDNILRTRTYD+SITYDKYYQTPRVWLTGYDESRMLL
Sbjct: 181 ADNVVENDPATLQSTYLVAHEPDDDNILRTRTYDLSITYDKYYQTPRVWLTGYDESRMLL 240

Query: 241 QPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHK 300
           QPE V+EDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEV K
Sbjct: 241 QPELVMEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVDK 300

Query: 301 YLFLFLKFVASVIPTIEYDYTMDVDLGGPS 331
           YLFLFLKF+ASVIPTIEYDYTMD DLG  S
Sbjct: 301 YLFLFLKFMASVIPTIEYDYTMDFDLGSSS 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WWQ15.2e-14777.58Autophagy-related protein 3 OS=Arabidopsis thaliana OX=3702 GN=ATG3 PE=1 SV=2[more]
Q550A86.5e-7345.43Autophagy-related protein 3 OS=Dictyostelium discoideum OX=44689 GN=atg3 PE=3 SV... [more]
Q9CPX69.3e-6445.54Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=... [more]
Q6PFS71.2e-6345.45Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1[more]
Q6AZ501.3e-6244.83Ubiquitin-like-conjugating enzyme ATG3 OS=Rattus norvegicus OX=10116 GN=Atg3 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1H5115.9e-17894.88autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460636 P... [more]
A0A6J1KTV36.5e-17794.28autophagy-related protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111498158 PE=... [more]
A0A1S3CAI39.4e-16890.00autophagy-related protein 3 OS=Cucumis melo OX=3656 GN=LOC103498815 PE=4 SV=1[more]
A0A6J1H4I99.4e-16889.46autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460417 P... [more]
A0A5A7T8K59.4e-16890.00Autophagy-related protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
Match NameE-valueIdentityDescription
XP_022959612.11.2e-17794.88autophagy-related protein 3-like [Cucurbita moschata][more]
KAG7025347.11.0e-17694.58Autophagy-related protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_023005051.11.3e-17694.28autophagy-related protein 3-like [Cucurbita maxima][more]
XP_023514388.12.3e-17694.28autophagy-related protein 3-like [Cucurbita pepo subsp. pepo][more]
KAG7013823.11.1e-16789.46Autophagy-related protein 3 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G61500.13.7e-14877.58autophagy 3 (APG3) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007135Ubiquitin-like-conjugating enzyme Atg3/Atg10PFAMPF03987Autophagy_act_Ccoord: 37..320
e-value: 2.9E-62
score: 210.3
IPR007135Ubiquitin-like-conjugating enzyme Atg3/Atg10PANTHERPTHR12866UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3coord: 1..326
NoneNo IPR availableGENE3D3.30.1460.50coord: 177..315
e-value: 3.5E-5
score: 25.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..143
NoneNo IPR availablePANTHERPTHR12866:SF2UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3coord: 1..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G001500.1CmoCh08G001500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000045 autophagosome assembly
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0044804 autophagy of nucleus
cellular_component GO:0000153 cytoplasmic ubiquitin ligase complex
cellular_component GO:0005829 cytosol
molecular_function GO:0019776 Atg8 ligase activity
molecular_function GO:0019787 ubiquitin-like protein transferase activity