CmoCh08G001160 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G001160
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCmo_Chr08: 602767 .. 605274 (+)
RNA-Seq ExpressionCmoCh08G001160
SyntenyCmoCh08G001160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACCCATCCGAAATTGGGTCTGAAAAAGGAAGGGAAAAGAAAAAACCCCTTCGATTTTTAACTAATTTCTCGTTTTCTTATTAATAAATTTCCCTTCTCTCAATTTCTCCGATCTCAAAACCGCCCATTTGAGCCCTCCGATCAACAATTTTCAATCATTTTTCCGATCATTCTTCAGATTCCGTCAAGCCGGCGATATTAATGGCGGAATCATCTGACCCATCAAAACCCATCAATGGATTCTGATGTTCTTCCGATTGTCGGAGCGGAATCATTAATCATTCCAAGTCGGAGAGGCTTATTATTTTCCGAAAATGGAGTTGGATGGCCTCGAACGCGCGCCGCCGTCCATGGACGTGATGAACGAGGAGGACAAGACCCTTCAATGTCACTGTCAGTTTCCGTCGATTTCCAAGCCTCGGATCAGCAATAACGACAATCCGACCACTACAAGTGTCCACGAGCTGCTGGAATGTCCTGTCTGCACTAATTCTATGTATCCTCCAATTCATCAGGTTCCTTCAAGTTCGTTGCTGTTTTTGTTTCAATTCGTTTGGTGTTTGTGGTTTCTGATCTGTTTGATTATGCTGAAGCTGTGGGTTTGTTGTTTCAGAAATGTTCTGTTTAATGAGGCCTTTGGTTCATGAAATGAGTATCAATTGTTTGGTTAAACCCTTGCTGTTGCATATACTCATTTGCAAAATGAATAGCTTTTTTTGTTGTTTAGAGACAATTGTTGCTGTGCTTTAGCTTGTTATGTATCCTCGTCAGCCTCACGGTTTTAAAATCCGTCTACTAGAGAGAGATTTCCACACCCTCCATTGGGGAAGTGGGGTTCCTCTGTGTGACCTTGTGTAACAAGCCTAGCAGATATTGTTCACTTTGGCTTGTTATGTATCGTCGTTAGTCTCACGGTTGTAAAATCCATCAACTAGAGAGAGATTTCCACACCCTCCATTGGGGAAGGGGGGCTCCTCTTTGTGACCTCGTGTAACAAGCCTAGCAGATATTGTTCGCTTTGACTTGTTATGTATCGTCGTCAGTCTCACGGTTTTAAAATTTGTCTACTTGGGAGAGATTTACACACCCTTGTGAAGATACGTAACGGGTCAAAATGGACCATATCAACTAGCGGTAGGCTTGGGCGGTTACAAATGGTATCAAAGTCAGACACCAAGCTGTTTGTTAGTGAGGATAATGAACCGTCAAGAGGAATGGATTGTGAGATCCCACGATGGTTAGAGAGGGGAACGAAACATTCCTTAAAGGGTGTGGAAACCTCTCCCTGGGCAAACACGTTTTAAGGTCGTGAGGTTGATAGCGATACGTAACGAGTCAAAGCAAACAAGATTTGTGAGCAGTGAACTTGGACTATAAGAGGATGTTTTTTATAACCAGGATTTTATTATTGAGAACTCGGATTCTACCTTGAGATCTGTGGCTTCATCAAATGAGAAGATATTGCTGAATGTTGTGAACTTATTGTTTTGTTTGTGCAGTGTCCCAATGGACATACCCTCTGTTCTTCGTGTAAAACGAAGGTAGACAACCGTTGCCCGACGTGCAGACAAGAGCTCGGTGATATTAGATGTCTAGCATTAGAAAAAATAGCCGAATCGCTTAAACTGGCTTGTAAATTCTGCACGTTCGGGTGCTCGGAGATATTGCCGTACTATAGTAAACTAAAACATGAATCTGCCTGTTACTTCAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAATTGATGGGGAGATTCCTTTCCTTGTTTCTCATCTTCGAGATGATCACAAAGTCGATATGCATTCTGGATGCACGTTTAATCATCGTTACGTAAAGGCTAATCCATGCGAAGTTGAAAACGCTATATGGATGTTAACTGTGAGTATCTGTTGAACGATTTCACGAGACGATATCCAAGATCACGTCTTACTTCTTACGCGAGCTTCTGTTGATGGCTCTTATTTCACCTTTGTGCAGGTCTTCCACTGTTTTGACCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTTGGTATGGCACCAGTTTACATGGTGTTTCTCCGATTCATGGGCGACGAAACAGACGCCCGAAACTACAGTTACAGCCTCGAGGCCGGTGGGAATGGGAGGAAGCTAACGTGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTCCGAGACAGCCACGATGGCCTCATTATACATCGAAACATGGCACTGTTCTTCTCGGGCGGGGAAAGGAAAGAGCTGAAGTTGCGGATCATGGGACGGATTTGGAAAGAACAAAATAAGCAGTGATATTGTTGTTGAATCAGCTCATAAGTCTTTGCCAATCCTAATAAACAGTGTACACAAAGGTCTTGAAACCATCTTCGACTCCAACTTCTTGTAATTGGGAATAGCTTACAACATTAAACATTGAAGCTTGTTTTGAAAACTGTGTTCTTAGTTGTTCTTTTTCCAATAAGAAAAAAAGAGCAGAAAAGACAAA

mRNA sequence

GACCCATCCGAAATTGGGTCTGAAAAAGGAAGGGAAAAGAAAAAACCCCTTCGATTTTTAACTAATTTCTCGTTTTCTTATTAATAAATTTCCCTTCTCTCAATTTCTCCGATCTCAAAACCGCCCATTTGAGCCCTCCGATCAACAATTTTCAATCATTTTTCCGATCATTCTTCAGATTCCGTCAAGCCGGCGATATTAATGGCGGAATCATCTGACCCATCAAAACCCATCAATGGATTCTGATGTTCTTCCGATTGTCGGAGCGGAATCATTAATCATTCCAAGTCGGAGAGGCTTATTATTTTCCGAAAATGGAGTTGGATGGCCTCGAACGCGCGCCGCCGTCCATGGACGTGATGAACGAGGAGGACAAGACCCTTCAATGTCACTGTCAGTTTCCGTCGATTTCCAAGCCTCGGATCAGCAATAACGACAATCCGACCACTACAAGTGTCCACGAGCTGCTGGAATGTCCTGTCTGCACTAATTCTATGTATCCTCCAATTCATCAGTGTCCCAATGGACATACCCTCTGTTCTTCGTGTAAAACGAAGGTAGACAACCGTTGCCCGACGTGCAGACAAGAGCTCGGTGATATTAGATGTCTAGCATTAGAAAAAATAGCCGAATCGCTTAAACTGGCTTGTAAATTCTGCACGTTCGGGTGCTCGGAGATATTGCCGTACTATAGTAAACTAAAACATGAATCTGCCTGTTACTTCAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAATTGATGGGGAGATTCCTTTCCTTGTTTCTCATCTTCGAGATGATCACAAAGTCGATATGCATTCTGGATGCACGTTTAATCATCGTTACGTAAAGGCTAATCCATGCGAAGTTGAAAACGCTATATGGATGTTAACTGTCTTCCACTGTTTTGACCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTTGGTATGGCACCAGTTTACATGGTGTTTCTCCGATTCATGGGCGACGAAACAGACGCCCGAAACTACAGTTACAGCCTCGAGGCCGGTGGGAATGGGAGGAAGCTAACGTGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTCCGAGACAGCCACGATGGCCTCATTATACATCGAAACATGGCACTGTTCTTCTCGGGCGGGGAAAGGAAAGAGCTGAAGTTGCGGATCATGGGACGGATTTGGAAAGAACAAAATAAGCAGTGATATTGTTGTTGAATCAGCTCATAAGTCTTTGCCAATCCTAATAAACAGTGTACACAAAGGTCTTGAAACCATCTTCGACTCCAACTTCTTGTAATTGGGAATAGCTTACAACATTAAACATTGAAGCTTGTTTTGAAAACTGTGTTCTTAGTTGTTCTTTTTCCAATAAGAAAAAAAGAGCAGAAAAGACAAA

Coding sequence (CDS)

ATGGAGTTGGATGGCCTCGAACGCGCGCCGCCGTCCATGGACGTGATGAACGAGGAGGACAAGACCCTTCAATGTCACTGTCAGTTTCCGTCGATTTCCAAGCCTCGGATCAGCAATAACGACAATCCGACCACTACAAGTGTCCACGAGCTGCTGGAATGTCCTGTCTGCACTAATTCTATGTATCCTCCAATTCATCAGTGTCCCAATGGACATACCCTCTGTTCTTCGTGTAAAACGAAGGTAGACAACCGTTGCCCGACGTGCAGACAAGAGCTCGGTGATATTAGATGTCTAGCATTAGAAAAAATAGCCGAATCGCTTAAACTGGCTTGTAAATTCTGCACGTTCGGGTGCTCGGAGATATTGCCGTACTATAGTAAACTAAAACATGAATCTGCCTGTTACTTCAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAATTGATGGGGAGATTCCTTTCCTTGTTTCTCATCTTCGAGATGATCACAAAGTCGATATGCATTCTGGATGCACGTTTAATCATCGTTACGTAAAGGCTAATCCATGCGAAGTTGAAAACGCTATATGGATGTTAACTGTCTTCCACTGTTTTGACCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTTGGTATGGCACCAGTTTACATGGTGTTTCTCCGATTCATGGGCGACGAAACAGACGCCCGAAACTACAGTTACAGCCTCGAGGCCGGTGGGAATGGGAGGAAGCTAACGTGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTCCGAGACAGCCACGATGGCCTCATTATACATCGAAACATGGCACTGTTCTTCTCGGGCGGGGAAAGGAAAGAGCTGAAGTTGCGGATCATGGGACGGATTTGGAAAGAACAAAATAAGCAGTGA

Protein sequence

MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWKEQNKQ
Homology
BLAST of CmoCh08G001160 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 6.2e-139
Identity = 231/315 (73.33%), Postives = 262/315 (83.17%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDN-----------PTTTSVH 60
           M+LD ++    +MDV ++E+     H  + S+SK   +NN+            PTTTSVH
Sbjct: 1   MDLDSMD-CTSTMDVTDDEEIHQDRH-SYASVSKHHHTNNNTTNVNAAASGLLPTTTSVH 60

Query: 61  ELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLK 120
           ELLECPVCTNSMYPPIHQC NGHTLCS+CK +V NRCPTCRQELGDIRCLALEK+AESL+
Sbjct: 61  ELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLE 120

Query: 121 LACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVD 180
           L CK  + GC EI PYYSKLKHE+ C FRPY+CPYAGS+C + G+IPFLV+HLRDDHKVD
Sbjct: 121 LPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVD 180

Query: 181 MHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGD 240
           MHSGCTFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGD
Sbjct: 181 MHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGD 240

Query: 241 ETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKE 300
           ET+ARNY+YSLE GG GRKL WEG PRS+RD+H+KVRDSHDGLII RNMALFFSGG+RKE
Sbjct: 241 ETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKE 300

Query: 301 LKLRIMGRIWKEQNK 305
           LKLR+ GRIWKEQ +
Sbjct: 301 LKLRVTGRIWKEQQQ 313

BLAST of CmoCh08G001160 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 469.2 bits (1206), Expect = 3.7e-131
Identity = 218/291 (74.91%), Postives = 245/291 (84.19%), Query Frame = 0

Query: 12  SMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNG 71
           S+D + ++D+  Q H QF S      +       TSV+ELLECPVCTNSMYPPIHQC NG
Sbjct: 5   SIDSVIDDDEIHQKH-QFSSTKSQGGATVVISPATSVYELLECPVCTNSMYPPIHQCHNG 64

Query: 72  HTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKH 131
           HTLCS+CK++V NRCPTCRQELGDIRCLALEK+AESL+L CK+   GC  I PYYSKLKH
Sbjct: 65  HTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYSKLKH 124

Query: 132 ESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENA 191
           ES C FRPY+CPYAGS+C   G+I FLV+HLRDDHKVDMH+GCTFNHRYVK+NP EVENA
Sbjct: 125 ESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENA 184

Query: 192 IWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSLEAGGNGRKLTW 251
            WMLTVF CF QYFCLHFEAFQLGMAPVYM FLRFMGDE DARNY+YSLE GG+GRK TW
Sbjct: 185 TWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTW 244

Query: 252 EGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWKEQ 303
           EG PRS+RD+H+KVRDSHDGLII RNMALFFSGG++KELKLR+ GRIWKEQ
Sbjct: 245 EGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQ 294

BLAST of CmoCh08G001160 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 4.8e-131
Identity = 216/271 (79.70%), Postives = 238/271 (87.82%), Query Frame = 0

Query: 37  ISNNDNPT----TTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQE 96
           ++N   PT     TSV+ELLECPVCT SMYPPIHQC NGHTLCS+CK +V NRCPTCRQE
Sbjct: 43  VTNIVGPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQE 102

Query: 97  LGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPID 156
           LGDIRCLALEK+AESL+L CKF   GC EI PYYSKLKHES C FRPY+CPYAGS+C I 
Sbjct: 103 LGDIRCLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIV 162

Query: 157 GEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAF 216
           G+IPFLV+HLRDDHKVDMH+G TFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAF
Sbjct: 163 GDIPFLVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAF 222

Query: 217 QLGMAPVYMVFLRFMGDETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGL 276
           QLGM PVYM FLRFMGDE DAR+YSYSLE GG+GRKLTWEG PRSIRD+H+KVRDS+DGL
Sbjct: 223 QLGMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGL 282

Query: 277 IIHRNMALFFSGGERKELKLRIMGRIWKEQN 304
           II RNMALFFSGG+RKELKLR+ G+IWKEQ+
Sbjct: 283 IIQRNMALFFSGGDRKELKLRVTGKIWKEQH 313

BLAST of CmoCh08G001160 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.1e-124
Identity = 206/293 (70.31%), Postives = 238/293 (81.23%), Query Frame = 0

Query: 10  PPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNSMYPPIHQCP 69
           PP  +V+   D T       P   KP +       ++SV ELLECPVC ++MYPPIHQC 
Sbjct: 17  PPKTEVL---DVTELAGDPVPHSPKPNV-----VVSSSVRELLECPVCLSAMYPPIHQCS 76

Query: 70  NGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKL 129
           NGHTLCS CK +V NRCPTCR ELG+IRCLALEK+A SL+L CK+  FGC  I PYY KL
Sbjct: 77  NGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQNFGCVGIYPYYCKL 136

Query: 130 KHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVE 189
           KHES C +RPY+CPYAGS+C + G+IP+LV+HL+DDHKVDMH+GCTFNHRYVK+NP EVE
Sbjct: 137 KHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDDHKVDMHNGCTFNHRYVKSNPHEVE 196

Query: 190 NAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSLEAGGNGRKL 249
           NA WMLTVF CF QYFCLHFEAFQLGMAPVY+ FLRFMGD+ +A+NYSYSLE GG GRK+
Sbjct: 197 NATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDLEAKNYSYSLEVGGTGRKM 256

Query: 250 TWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWKEQ 303
            W+G PRSIRD+H+KVRDS+DGLII RNMALFFSGGERKELKLR+ GRIWKEQ
Sbjct: 257 IWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGGERKELKLRVTGRIWKEQ 301

BLAST of CmoCh08G001160 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 1.5e-121
Identity = 193/259 (74.52%), Postives = 224/259 (86.49%), Query Frame = 0

Query: 44  TTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEK 103
           +   V+ELLECPVCTN MYPPIHQCPNGHTLCS+CK +V N CPTCR ELG+IRCLALEK
Sbjct: 50  SNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEK 109

Query: 104 IAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLR 163
           +AESL++ C++   GC +I PYYSKLKHE  C FRPYTCPYAGS+C + G+IP LV HL+
Sbjct: 110 VAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLK 169

Query: 164 DDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVF 223
           DDHKVDMH GCTFNHRYVK+NP EVENA WMLTVF+CF + FCLHFEAFQLGMAPVYM F
Sbjct: 170 DDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAF 229

Query: 224 LRFMGDETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFS 283
           LRFMGDE +A+ +SYSLE G +GRKLTW+G PRSIRD+H+KVRDS DGLII RN+AL+FS
Sbjct: 230 LRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFS 289

Query: 284 GGERKELKLRIMGRIWKEQ 303
           GG+R+ELKLR+ GRIWKE+
Sbjct: 290 GGDRQELKLRVTGRIWKEE 308

BLAST of CmoCh08G001160 vs. ExPASy TrEMBL
Match: A0A6J1HB87 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461228 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 5.4e-186
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK
Sbjct: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 305

BLAST of CmoCh08G001160 vs. ExPASy TrEMBL
Match: A0A6J1KS44 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498159 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.0e-184
Identity = 301/305 (98.69%), Postives = 303/305 (99.34%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG ERAPPSMDVM+EEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCP+DGEIPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E GGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK
Sbjct: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 305

BLAST of CmoCh08G001160 vs. ExPASy TrEMBL
Match: A0A6J1JVF7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488681 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.2e-174
Identity = 287/305 (94.10%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG+E   PSMDVM+EEDKTLQ HCQFPSISKPRI+NNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGIE-GVPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKT+VDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGC 
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCP 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E GGNGRKL WEGNPRSIRDNHKKVRDSHDGLII RNMALFFSGGERKELKLRI GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 304

BLAST of CmoCh08G001160 vs. ExPASy TrEMBL
Match: A0A6J1ENP4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111436299 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.2e-174
Identity = 287/305 (94.10%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG+E   PSMDVM+EEDKTLQ HCQFPSISKPRI+NNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGIE-GVPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKT+VDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGC 
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCP 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E GGNGRKL WEGNPRSIRDNHKKVRDSHDGLII RNMALFFSGGERKELKLRI GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 304

BLAST of CmoCh08G001160 vs. ExPASy TrEMBL
Match: A0A6J1DUC0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111024411 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 7.6e-172
Identity = 282/305 (92.46%), Postives = 292/305 (95.74%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG+E   PSMDVM+EED TLQ HCQFPSISKPRI+NNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGIE-GVPSMDVMDEEDTTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCS+CKT+VDNRCPTCRQELGDIRCLALEKIAESLKLACKFCT+GC 
Sbjct: 61  MYPPIHQCPNGHTLCSTCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTYGCP 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHE+ACYFRPYTCPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHEAACYFRPYTCPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E GGNGRKL WEGNPRSIRDNHKKVRDSHDGLII RNMALFFSGGE+KELKLRI GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGEKKELKLRITGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 304

BLAST of CmoCh08G001160 vs. NCBI nr
Match: XP_022960514.1 (E3 ubiquitin-protein ligase SINAT3-like [Cucurbita moschata])

HSP 1 Score: 659.8 bits (1701), Expect = 1.1e-185
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK
Sbjct: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 305

BLAST of CmoCh08G001160 vs. NCBI nr
Match: KAG6592906.1 (E3 ubiquitin-protein ligase SINAT3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025311.1 E3 ubiquitin-protein ligase SINAT3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 658.3 bits (1697), Expect = 3.2e-185
Identity = 304/305 (99.67%), Postives = 304/305 (99.67%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E GGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK
Sbjct: 241 EVGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 305

BLAST of CmoCh08G001160 vs. NCBI nr
Match: XP_023513424.1 (E3 ubiquitin-protein ligase SINAT3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.9 bits (1696), Expect = 4.2e-185
Identity = 303/305 (99.34%), Postives = 304/305 (99.67%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCP+DGEIPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E GGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK
Sbjct: 241 EVGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 305

BLAST of CmoCh08G001160 vs. NCBI nr
Match: XP_023005052.1 (E3 ubiquitin-protein ligase SINAT3-like [Cucurbita maxima])

HSP 1 Score: 654.1 bits (1686), Expect = 6.1e-184
Identity = 301/305 (98.69%), Postives = 303/305 (99.34%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG ERAPPSMDVM+EEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCP+DGEIPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E GGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK
Sbjct: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 305

BLAST of CmoCh08G001160 vs. NCBI nr
Match: XP_022929797.1 (E3 ubiquitin-protein ligase SINAT3-like [Cucurbita moschata] >XP_022992340.1 E3 ubiquitin-protein ligase SINAT3-like [Cucurbita maxima] >XP_023548870.1 E3 ubiquitin-protein ligase SINAT3-like [Cucurbita pepo subsp. pepo] >KAG6575290.1 E3 ubiquitin-protein ligase SINAT3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013845.1 E3 ubiquitin-protein ligase SINAT3 [Cucurbita argyrosperma subsp. argyrosperma] >KAG6575314.1 E3 ubiquitin-protein ligase SINAT3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.8 bits (1597), Expect = 1.3e-173
Identity = 287/305 (94.10%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG+E   PSMDVM+EEDKTLQ HCQFPSISKPRI+NNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGIE-GVPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKT+VDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGC 
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCP 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACYFRPYTCPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDETDARNYSYSL
Sbjct: 181 VKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSL 240

Query: 241 EAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E GGNGRKL WEGNPRSIRDNHKKVRDSHDGLII RNMALFFSGGERKELKLRI GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 304

BLAST of CmoCh08G001160 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 495.0 bits (1273), Expect = 4.4e-140
Identity = 231/315 (73.33%), Postives = 262/315 (83.17%), Query Frame = 0

Query: 1   MELDGLERAPPSMDVMNEEDKTLQCHCQFPSISKPRISNNDN-----------PTTTSVH 60
           M+LD ++    +MDV ++E+     H  + S+SK   +NN+            PTTTSVH
Sbjct: 1   MDLDSMD-CTSTMDVTDDEEIHQDRH-SYASVSKHHHTNNNTTNVNAAASGLLPTTTSVH 60

Query: 61  ELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLK 120
           ELLECPVCTNSMYPPIHQC NGHTLCS+CK +V NRCPTCRQELGDIRCLALEK+AESL+
Sbjct: 61  ELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLE 120

Query: 121 LACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLRDDHKVD 180
           L CK  + GC EI PYYSKLKHE+ C FRPY+CPYAGS+C + G+IPFLV+HLRDDHKVD
Sbjct: 121 LPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVD 180

Query: 181 MHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGD 240
           MHSGCTFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGD
Sbjct: 181 MHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGD 240

Query: 241 ETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKE 300
           ET+ARNY+YSLE GG GRKL WEG PRS+RD+H+KVRDSHDGLII RNMALFFSGG+RKE
Sbjct: 241 ETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKE 300

Query: 301 LKLRIMGRIWKEQNK 305
           LKLR+ GRIWKEQ +
Sbjct: 301 LKLRVTGRIWKEQQQ 313

BLAST of CmoCh08G001160 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 468.8 bits (1205), Expect = 3.4e-132
Identity = 216/271 (79.70%), Postives = 238/271 (87.82%), Query Frame = 0

Query: 37  ISNNDNPT----TTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQE 96
           ++N   PT     TSV+ELLECPVCT SMYPPIHQC NGHTLCS+CK +V NRCPTCRQE
Sbjct: 43  VTNIVGPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQE 102

Query: 97  LGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPID 156
           LGDIRCLALEK+AESL+L CKF   GC EI PYYSKLKHES C FRPY+CPYAGS+C I 
Sbjct: 103 LGDIRCLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIV 162

Query: 157 GEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAF 216
           G+IPFLV+HLRDDHKVDMH+G TFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAF
Sbjct: 163 GDIPFLVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAF 222

Query: 217 QLGMAPVYMVFLRFMGDETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGL 276
           QLGM PVYM FLRFMGDE DAR+YSYSLE GG+GRKLTWEG PRSIRD+H+KVRDS+DGL
Sbjct: 223 QLGMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGL 282

Query: 277 IIHRNMALFFSGGERKELKLRIMGRIWKEQN 304
           II RNMALFFSGG+RKELKLR+ G+IWKEQ+
Sbjct: 283 IIQRNMALFFSGGDRKELKLRVTGKIWKEQH 313

BLAST of CmoCh08G001160 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 437.2 bits (1123), Expect = 1.1e-122
Identity = 193/259 (74.52%), Postives = 224/259 (86.49%), Query Frame = 0

Query: 44  TTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEK 103
           +   V+ELLECPVCTN MYPPIHQCPNGHTLCS+CK +V N CPTCR ELG+IRCLALEK
Sbjct: 50  SNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEK 109

Query: 104 IAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLR 163
           +AESL++ C++   GC +I PYYSKLKHE  C FRPYTCPYAGS+C + G+IP LV HL+
Sbjct: 110 VAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLK 169

Query: 164 DDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVF 223
           DDHKVDMH GCTFNHRYVK+NP EVENA WMLTVF+CF + FCLHFEAFQLGMAPVYM F
Sbjct: 170 DDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAF 229

Query: 224 LRFMGDETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFS 283
           LRFMGDE +A+ +SYSLE G +GRKLTW+G PRSIRD+H+KVRDS DGLII RN+AL+FS
Sbjct: 230 LRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFS 289

Query: 284 GGERKELKLRIMGRIWKEQ 303
           GG+R+ELKLR+ GRIWKE+
Sbjct: 290 GGDRQELKLRVTGRIWKEE 308

BLAST of CmoCh08G001160 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 429.5 bits (1103), Expect = 2.3e-120
Identity = 189/259 (72.97%), Postives = 222/259 (85.71%), Query Frame = 0

Query: 44  TTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEK 103
           ++  V+ELLECPVCTN MYPPIHQCPNGHTLCSSCK +V N CPTCR ELG+IRCLALEK
Sbjct: 47  SSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEK 106

Query: 104 IAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFLVSHLR 163
           +AESL++ C++   GC +I PYYSKLKHE  C FR Y+CPYAGS+C + G+IP LV HL+
Sbjct: 107 VAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLK 166

Query: 164 DDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVF 223
           DDHKVDMH GCTFNHRYVK+NP EVENA WMLTVF+CF + FCLHFEAFQLGMAPVYM F
Sbjct: 167 DDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAF 226

Query: 224 LRFMGDETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFS 283
           LRFMGDE +A+ +SYSLE G + RKLTW+G PRSIRD+H+KVRDS DGLII RN+AL+FS
Sbjct: 227 LRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFS 286

Query: 284 GGERKELKLRIMGRIWKEQ 303
           G +++ELKLR+ GRIWKE+
Sbjct: 287 GSDKEELKLRVTGRIWKEE 305

BLAST of CmoCh08G001160 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 358.2 bits (918), Expect = 6.5e-99
Identity = 162/204 (79.41%), Postives = 180/204 (88.24%), Query Frame = 0

Query: 99  LALEKIAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPIDGEIPFL 158
           +ALEK+AESL+L CK+   GC  I PYYSKLKHES C FRPY+CPYAGS+C   G+I FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 159 VSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAP 218
           V+HLRDDHKVDMH+GCTFNHRYVK+NP EVENA WMLTVF CF QYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 219 VYMVFLRFMGDETDARNYSYSLEAGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNM 278
           VYM FLRFMGDE DARNY+YSLE GG+GRK TWEG PRS+RD+H+KVRDSHDGLII RNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 279 ALFFSGGERKELKLRIMGRIWKEQ 303
           ALFFSGG++KELKLR+ GRIWKEQ
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84JL36.2e-13973.33E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q8S3N13.7e-13174.91E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q9STN84.8e-13179.70E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q10L911.1e-12470.31E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q9M2P41.5e-12174.52E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1HB875.4e-186100.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461228... [more]
A0A6J1KS443.0e-18498.69RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498159 P... [more]
A0A6J1JVF76.2e-17494.10RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488681 P... [more]
A0A6J1ENP46.2e-17494.10RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111436299... [more]
A0A6J1DUC07.6e-17292.46RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102441... [more]
Match NameE-valueIdentityDescription
XP_022960514.11.1e-185100.00E3 ubiquitin-protein ligase SINAT3-like [Cucurbita moschata][more]
KAG6592906.13.2e-18599.67E3 ubiquitin-protein ligase SINAT3, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023513424.14.2e-18599.34E3 ubiquitin-protein ligase SINAT3-like [Cucurbita pepo subsp. pepo][more]
XP_023005052.16.1e-18498.69E3 ubiquitin-protein ligase SINAT3-like [Cucurbita maxima][more]
XP_022929797.11.3e-17394.10E3 ubiquitin-protein ligase SINAT3-like [Cucurbita moschata] >XP_022992340.1 E3 ... [more]
Match NameE-valueIdentityDescription
AT3G61790.14.4e-14073.33Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.13.4e-13279.70Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.11.1e-12274.52seven in absentia of Arabidopsis 2 [more]
AT2G41980.12.3e-12072.97Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.16.5e-9979.41TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 38..97
e-value: 1.3E-6
score: 29.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 102..162
e-value: 3.7E-14
score: 54.7
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 96..294
e-value: 7.4E-75
score: 251.3
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 166..295
e-value: 1.8E-36
score: 126.8
NoneNo IPR availablePANTHERPTHR10315:SF125UBIQUITIN LIGASE SINAT3coord: 12..303
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 12..303
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 52..90
e-value: 5.89395E-14
score: 63.0726
NoneNo IPR availableCDDcd03829Sinacoord: 168..296
e-value: 2.56683E-61
score: 189.135
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 107..298
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 48..94
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 107..167
score: 13.62236
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 54..90
score: 9.89275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G001160.1CmoCh08G001160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding