CmoCh08G000720 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G000720
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionProtein kinase domain-containing protein
LocationCmo_Chr08: 389249 .. 393807 (+)
RNA-Seq ExpressionCmoCh08G000720
SyntenyCmoCh08G000720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGACACGTCCTAAACCCAACCCCATTATCTTCCCATGACATAGGAACGATTAGAGGTCATATTATGTAAGATCCCACATTGGCTGGAGGGAGAAATGAAACATTTCTAATAAGCGTGTGAATGGTATTAGAGCTAGAGGTAGGCTTGGGTTGTTGCAAATGGTATCAGAGCTAGACACTGGGCTGTGTGCCAACGAGGACGCTGGCCCCTGTTGAGGATTATGGGGAGCGAGTCCCACATTGGCTAATTTAGGGAATAATCGTGGGTTTATAACTAAAGGAATATATCTCCATTGGTACGAGGCTTTTTGAGGAAGCACAAAGCAAAGCCACGAGAGCTTATGCTCGAAGTGGACAATATCATACCATTGTTGAGAGTCGTGATTCCTAATAGCCCCTAAGGAGGTGGATTGTGAGATCTTACATCAGTTGGAGAGGATAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCCCGAGATCTTACATTCATTGAGCTTCTAAAAATGACTAAGTTTACATATATGTACAGAAAATACAAAAGGAAACCAAGTGCAAAATGAAGCAGAGAAAGAAAATGAGATTGGAGGAAAGAACAACATTGCAGCAAAAACCTTCACGTTCAGAGAGCTGGCCACTGCCACGAAGAATTTCCGCGAAGAGTGTCTGATCGGAGAGGGCGGGTTCGGGAGAGTTTACAAGGGCAAACTCGACAAAATTTCCCAGGTACCATATTCATCGTCCCGTCTCCCGTTTTCCTCGTTTGATAATCATTTTGTTTATCTTTAAGACGACGCGTTATAGATGAGTATAAGAGAGTGTAGTTTTGGCATGTCACAGGTTGTAGCAGTGAAGCAGCTTGATAGGAATGGACTGCAAGGGAATAGAGAGTTCCTTGTGGAGGTTTTGATGCTAAGCCTTTTACACCATAAGAACCTTGTCAATCTCATTGGGTATTGTGCAGATGGTGATCAAAGACTCTTGGTCTATGAGTATATGCCTTTGGGATCTCTAGAGGATCATCTACTTGGTAATAAACATATATAAAACCTAACCTTTCATACTTAGCTGTGTTTAATGGTGAGATCTCACGTTGGTTGGAGAGAGCAGCGGAGCAGCGAAACATTCCTTATAAGGGTGTAGAGACCTCTCCCTCGTAGATGTGTTTTAAAACTGTGAGGTTGATAATATTTGCTAGCGATAAGCTTCTGCTGTTACAAATGATATCAGAGCCAGTCATGGGGCGGTGTGTCTAGTGAGGACGCTGGCCCCAATGGGGTGGATTGTGAGATCCCACCTTGGTCGAAGAGAGGAACAAAGCATTTACTTATAAAGGTGTGGAAACCTCTCCCTATTAGACACGTTTTACAACCATGAGACTGACAACGATAAGTAACGAGCCAAAGTGGACAATATCTTCTAGTAGTAGGCTTGGGCTCTTACAAATGTTATCGGAGACAGTCGCTAGGTGGCGTCCCAACGAGGACGCTAGCCCCCAAAATGGTGGATTGTTAGATCCCACATGGTTGGAGGAGAAGGGAACGAAACATTCCTTATAAGGGTGTGGAAACCTGTCCCTCGTAGTTGCATTTTTAAAACTATGAGGTTGACAATATTTGCTAGCGGTGGGCTTGGGCTGTTATAAATGGTATCAGAGCCAGTCACGATGCGGTGTACCAGTGAGGACACTGGGCCCAATGGGGTAGATTGTGAGATCTCACATCAGTTGAAAAGGGGAACAAAGCATTTCTTTTAAAGGTGTTGAAACCTCTCCTTATTAGACGCGTTTTACAACCATGAGACTGACGGCGGTACGTAACGAGCCAAAGTGGATAATATCTCTCTAGCGGTAGGCTTGGGCTATTATAAATGGTATCAGAGACAGTCACTGAGTGACGTGCTAACAAGGATGCTGGCCCCCAAGAGGGTGGATTGTGAGATCCCACGTCGGTTGGGGAAGGAAACGAAACATTTCTTATAAGAGTGTGTAAACCTTTTCCTAACAGACACGTTTTAAAACCATAACGGGTTAAAGCGGATAATATCTGTTAGCAGTCGGCTTGAACTGTTACATTGATCTTCCATTATCGTTTCAAACAAATGACTCGAGCTGTGTTTAATGATTTGGCATAGACCTTCCAGCGGATAGGAAGGCATTAGATTGGGCAACAAGAATGAAAGTAGCATTGGGAGCAGCAAAAGGGCTAGAGTACTTGCACGACAAAGCCAACCCACCTGTGATATATAGAGATTTGAAAGCATCAAACATTCTACTGGACAATGATTTCAATGCAAAGCTGTCGGATTTCGGGCTCGCAAAGCTTGGGCCTGTCGGGGACAAGTCCCATGTTTCCTCCAGGGTTATGGGAACCTATGGATATTGTGCTCCCGAGTATCAAAGAACAGGCCAGCTCACTCTCAAATCAGATGTCTACAGCTTTGGCGTCGTCTTGTTGGAACTCATCACCGGCAAGAGAGTCATCGACGACACTCGTCCAGCTAACCAGCAAAACCTGGTCGCTTGGGTAAGCCATTGAATCGACATGATTAAAAAAAAACATTACATATGTTAAATGAACACGACTCTCCACAATGGTATGATATTGCCCACTTTGAGCATAAGCTCTCGTAGCTTTGGTTTGGGCTTCCCAAGAAGCCTCATACCAACAGAGATAGTATTATTTGATTATAAACTCGACAATTCCCTGAAATTAGCAGATGTGGACCATCCAACAACATAAACGAACATATTTCATTTTCATGCATGCAGGCATGCCCAATATTCAAGGAACCAACTAAATTCATGGACCTGGCCGATCCTCTTTTGGGTAGGGACTTCCCTGTGAAATCTCTAAACCAAGCCGTCGCGGTGGCTGCAATGTGCCTCCACGAGGAAGCGACGGTGCGACCATTGATCAGCGATGTCGTCACAGCTCTTAGCTTCTTGGGTGCAGAAGCCACGCACTCGTCGTCTTTGTCGTTGTCGTCGATCGATGATCCTTCATCGGTAGTATCTGATCAAAATTTGATCAACCAATCAAAATCCAACGACGAGGAAATAGCCGAGGCACGCCAGCGAGCAGTCGCCGAAGCCATCGAATGGGGTTCCTCCTCCAAGCAAAATGCCGCCGCCTCGCGCCGGGGAAGTTGTTCTTCCTTGTAAATTTGTTAAGAACAGATATGCACAGTGTGGTGTTGATTTTACATGTATAGTATTGGATTCTCCCCAATGTGACGATGAGATACAAAAACGGTAAAAGCGGGGTAGGGTAATTGTAGGATTAAGAATTCAAAGCTCTGACCTTTCGATCGAGATTATAATTATTTTAGCTAATGAAGTTACGTTTCGGTTGATGAACCATTACTCCTCGAGAACTTTTCAAGGGCCCAATTTTATAGTAATTGATAGTCGAGATTGATCCTACCAAGACGACTCTCATTCAATGTGAGAGACAGAAATAATTTCACGTCGAGTTGTAATGATCTTGTCGAATAGATTCTTCTAAAATAAAGTTCGAGATGACTTCTTAAAACACCTCGATAACTGATTCATGTTTATTTCCGAGCTCACCAATTGAGTTTTACCGTTCTTTTTGCGATTCTTATGATGTTAAAGAAATAAAACAAAAAGGGGTCGAAGGTGTAACGAGATTAAGAATTAAGATTATCTAGAAAAGAGTCGCAAAAAATTCTCACAACATAGAGATTCTTTAGACAAAGACGGTCAACGCTCGTTTCATATAAGATTATATATATAGCTCATTTTAAACAAAAACGAGCACTAATTAAGACTTATATATATAGATAATAAAATTTAAAGATATATATATTTTTAATAATTCAAGAATATTTAGTTTTTTATTTTTTATTTTATTTTAAAGTCAACGTAAAATTAGTATAAAATCAATTGGTATTGACTTTTCAAACGTAAAATTAGTATAATATTTAGTTTTTTAGTTTTTTAATAAATGTTGACTTTTCAAACGCTTGCCTTTCAAGGAAAGAAAGAACGGTGGAATGGTAGATTCTAGAAAGTCGTAGACAATTCCAATGGTCCAGTCGACTTTTCCAATAATATATGCGCTTCCAAGTTCTACCGAACAAATCATTCTTACGTTTTGCATTTTTTAGCCAAGACATCGCGCGCCTCTCTTCCGCGACCTTGCTTCTCAGGTTCGTCTTTCTCTCGCTTTTCGATCAATTTTGTTCTTCTTTTTTCGCATTTCATCTTTTGATTCGGAATATTTTTGGTGGATCTTTTACAATTGTTTCTAATTTTCCCTTCATTTGGGATTTTTTGAATTTGAATCGGAGTTGGGGCTTTTTTCAGCCATGGAATTCTCTAGAACGCATAATTATTCAATGGATCATAATGCAGATCTTTTCTTGTATTTGCCCTATATTTTGAATTGGAAGAAGCATATGACGTTAACGATAATTTTGAGTTTCCGGTTCTCTTGATAATGTTGGGAGAAATATATCTTGATGCGTTGGTGATTTGCGTAGGTTATCCGTCTCTTATTCCTGCTGATTTTGCTTCCTTGAGCAATTGA

mRNA sequence

ATGGAAAGACACGTCCTAAACCCAACCCCATTATCTTCCCATGACATAGGAACGATTAGAGAAAATACAAAAGGAAACCAAGTGCAAAATGAAGCAGAGAAAGAAAATGAGATTGGAGGAAAGAACAACATTGCAGCAAAAACCTTCACGTTCAGAGAGCTGGCCACTGCCACGAAGAATTTCCGCGAAGAGTGTCTGATCGGAGAGGGCGGGTTCGGGAGAGTTTACAAGGGCAAACTCGACAAAATTTCCCAGGTTGTAGCAGTGAAGCAGCTTGATAGGAATGGACTGCAAGGGAATAGAGAGTTCCTTGTGGAGGTTTTGATGCTAAGCCTTTTACACCATAAGAACCTTGTCAATCTCATTGGGTATTGTGCAGATGGTGATCAAAGACTCTTGGTCTATGAGTATATGCCTTTGGGATCTCTAGAGGATCATCTACTTGACCTTCCAGCGGATAGGAAGGCATTAGATTGGGCAACAAGAATGAAAGTAGCATTGGGAGCAGCAAAAGGGCTAGAGTACTTGCACGACAAAGCCAACCCACCTGTGATATATAGAGATTTGAAAGCATCAAACATTCTACTGGACAATGATTTCAATGCAAAGCTGTCGGATTTCGGGCTCGCAAAGCTTGGGCCTGTCGGGGACAAGTCCCATGTTTCCTCCAGGGTTATGGGAACCTATGGATATTGTGCTCCCGAGTATCAAAGAACAGGCCAGCTCACTCTCAAATCAGATGTCTACAGCTTTGGCGTCGTCTTGTTGGAACTCATCACCGGCAAGAGAGTCATCGACGACACTCGTCCAGCTAACCAGCAAAACCTGGTCGCTTGGGCATGCCCAATATTCAAGGAACCAACTAAATTCATGGACCTGGCCGATCCTCTTTTGGGTAGGGACTTCCCTGTGAAATCTCTAAACCAAGCCGTCGCGGTGGCTGCAATGTGCCTCCACGAGGAAGCGACGGTGCGACCATTGATCAGCGATGTCGTCACAGCTCTTAGCTTCTTGGGTGCAGAAGCCACGCACTCGTCGTCTTTGTCGTTGTCGTCGATCGATGATCCTTCATCGGTAGTATCTGATCAAAATTTGATCAACCAATCAAAATCCAACGACGAGGAAATAGCCGAGGCACGCCAGCGAGCAGTCGCCGAAGCCATCGAATGGGGTTCCTCCTCCAAGCAAAATGCCGCCGCCTCGCGCCGGGGAACCAAGACATCGCGCGCCTCTCTTCCGCGACCTTGCTTCTCAGGTTATCCGTCTCTTATTCCTGCTGATTTTGCTTCCTTGAGCAATTGA

Coding sequence (CDS)

ATGGAAAGACACGTCCTAAACCCAACCCCATTATCTTCCCATGACATAGGAACGATTAGAGAAAATACAAAAGGAAACCAAGTGCAAAATGAAGCAGAGAAAGAAAATGAGATTGGAGGAAAGAACAACATTGCAGCAAAAACCTTCACGTTCAGAGAGCTGGCCACTGCCACGAAGAATTTCCGCGAAGAGTGTCTGATCGGAGAGGGCGGGTTCGGGAGAGTTTACAAGGGCAAACTCGACAAAATTTCCCAGGTTGTAGCAGTGAAGCAGCTTGATAGGAATGGACTGCAAGGGAATAGAGAGTTCCTTGTGGAGGTTTTGATGCTAAGCCTTTTACACCATAAGAACCTTGTCAATCTCATTGGGTATTGTGCAGATGGTGATCAAAGACTCTTGGTCTATGAGTATATGCCTTTGGGATCTCTAGAGGATCATCTACTTGACCTTCCAGCGGATAGGAAGGCATTAGATTGGGCAACAAGAATGAAAGTAGCATTGGGAGCAGCAAAAGGGCTAGAGTACTTGCACGACAAAGCCAACCCACCTGTGATATATAGAGATTTGAAAGCATCAAACATTCTACTGGACAATGATTTCAATGCAAAGCTGTCGGATTTCGGGCTCGCAAAGCTTGGGCCTGTCGGGGACAAGTCCCATGTTTCCTCCAGGGTTATGGGAACCTATGGATATTGTGCTCCCGAGTATCAAAGAACAGGCCAGCTCACTCTCAAATCAGATGTCTACAGCTTTGGCGTCGTCTTGTTGGAACTCATCACCGGCAAGAGAGTCATCGACGACACTCGTCCAGCTAACCAGCAAAACCTGGTCGCTTGGGCATGCCCAATATTCAAGGAACCAACTAAATTCATGGACCTGGCCGATCCTCTTTTGGGTAGGGACTTCCCTGTGAAATCTCTAAACCAAGCCGTCGCGGTGGCTGCAATGTGCCTCCACGAGGAAGCGACGGTGCGACCATTGATCAGCGATGTCGTCACAGCTCTTAGCTTCTTGGGTGCAGAAGCCACGCACTCGTCGTCTTTGTCGTTGTCGTCGATCGATGATCCTTCATCGGTAGTATCTGATCAAAATTTGATCAACCAATCAAAATCCAACGACGAGGAAATAGCCGAGGCACGCCAGCGAGCAGTCGCCGAAGCCATCGAATGGGGTTCCTCCTCCAAGCAAAATGCCGCCGCCTCGCGCCGGGGAACCAAGACATCGCGCGCCTCTCTTCCGCGACCTTGCTTCTCAGGTTATCCGTCTCTTATTCCTGCTGATTTTGCTTCCTTGAGCAATTGA

Protein sequence

MERHVLNPTPLSSHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRRGTKTSRASLPRPCFSGYPSLIPADFASLSN
Homology
BLAST of CmoCh08G000720 vs. ExPASy Swiss-Prot
Match: Q9SFT7 (Probable serine/threonine-protein kinase PBL26 OS=Arabidopsis thaliana OX=3702 GN=PBL26 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.2e-149
Identity = 275/381 (72.18%), Postives = 309/381 (81.10%), Query Frame = 0

Query: 18  TIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYK 77
           T+ E  K N    E          NNIAA+TF+FRELATATKNFR+ECLIGEGGFGRVYK
Sbjct: 44  TVNEQNKNNDEDKEV--------TNNIAAQTFSFRELATATKNFRQECLIGEGGFGRVYK 103

Query: 78  GKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEY 137
           GKL+K   +VAVKQLDRNGLQGN+EF+VEVLMLSLLHHK+LVNLIGYCADGDQRLLVYEY
Sbjct: 104 GKLEKTGMIVAVKQLDRNGLQGNKEFIVEVLMLSLLHHKHLVNLIGYCADGDQRLLVYEY 163

Query: 138 MPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLD 197
           M  GSLEDHLLDL  D+  LDW TR+++ALGAA GLEYLHDKANPPVIYRDLKA+NILLD
Sbjct: 164 MSRGSLEDHLLDLTPDQIPLDWDTRIRIALGAAMGLEYLHDKANPPVIYRDLKAANILLD 223

Query: 198 NDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLE 257
            +FNAKLSDFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLE
Sbjct: 224 GEFNAKLSDFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTTKSDVYSFGVVLLE 283

Query: 258 LITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMC 317
           LITG+RVID TRP ++QNLV WA P+FKEP++F +LADP L   FP K+LNQAVAVAAMC
Sbjct: 284 LITGRRVIDTTRPKDEQNLVTWAQPVFKEPSRFPELADPSLEGVFPEKALNQAVAVAAMC 343

Query: 318 LHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNDEEIA 377
           L EEATVRPL+SDVVTAL FLG       S+S+   DDP    SD+  +      D   A
Sbjct: 344 LQEEATVRPLMSDVVTALGFLG--TAPDGSISVPHYDDPPQ-PSDETSV-----EDSVAA 403

Query: 378 EARQRAVAEAIEWGSSSKQNA 399
           E R+RAVAEA+EWG +S+ ++
Sbjct: 404 EERERAVAEAMEWGVASRAHS 408

BLAST of CmoCh08G000720 vs. ExPASy Swiss-Prot
Match: Q9LRY1 (Probable serine/threonine-protein kinase PBL25 OS=Arabidopsis thaliana OX=3702 GN=PBL25 PE=2 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 7.7e-143
Identity = 258/365 (70.68%), Postives = 298/365 (81.64%), Query Frame = 0

Query: 31  EAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKLDKISQVVAVK 90
           E   +N  G  NN+ A+ FTFRELATATKNFR+ECLIGEGGFGRVYKGKL+  +QVVAVK
Sbjct: 35  EVVAKNANGPSNNMGARIFTFRELATATKNFRQECLIGEGGFGRVYKGKLENPAQVVAVK 94

Query: 91  QLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDL 150
           QLDRNGLQG REFLVEVLMLSLLHH+NLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDL
Sbjct: 95  QLDRNGLQGQREFLVEVLMLSLLHHRNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDL 154

Query: 151 PADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLSDFGLA 210
              +K LDW TR+K+ALGAAKG+EYLHD+A+PPVIYRDLK+SNILLD ++ AKLSDFGLA
Sbjct: 155 EPGQKPLDWNTRIKIALGAAKGIEYLHDEADPPVIYRDLKSSNILLDPEYVAKLSDFGLA 214

Query: 211 KLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVIDDTRP 270
           KLGPVGD  HVSSRVMGTYGYCAPEYQRTG LT KSDVYSFGVVLLELI+G+RVID  RP
Sbjct: 215 KLGPVGDTLHVSSRVMGTYGYCAPEYQRTGYLTNKSDVYSFGVVLLELISGRRVIDTMRP 274

Query: 271 ANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMCLHEEATVRPLISD 330
           +++QNLV WA PIF++PT++  LADPLL  D+P KSLNQA+AVAAMCLHEE TVRPL+SD
Sbjct: 275 SHEQNLVTWALPIFRDPTRYWQLADPLLRGDYPEKSLNQAIAVAAMCLHEEPTVRPLMSD 334

Query: 331 VVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNDEEIAEARQRAVAEAIEW 390
           V+TALSFLGA +  S++ S              N + Q++SN  +          +A++W
Sbjct: 335 VITALSFLGASSNSSNTGS--------------NHLQQNRSNKYQ----------DAVQW 375

Query: 391 GSSSK 396
            SS +
Sbjct: 395 DSSPR 375

BLAST of CmoCh08G000720 vs. ExPASy Swiss-Prot
Match: Q1PE89 (Probable serine/threonine-protein kinase PBL24 OS=Arabidopsis thaliana OX=3702 GN=PBL24 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 2.5e-133
Identity = 239/332 (71.99%), Postives = 276/332 (83.13%), Query Frame = 0

Query: 43  NIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNRE 102
           NI AK+F FRELATAT +FR+E LIGEGGFGRVYKGK++K  QVVAVKQLDRNGLQGNRE
Sbjct: 53  NIKAKSFKFRELATATNSFRQEFLIGEGGFGRVYKGKMEKTGQVVAVKQLDRNGLQGNRE 112

Query: 103 FLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATR 162
           FLVE+  LSLLHH NL NLIGYC DGDQRLLV+E+MPLGSLEDHLLD+   ++ LDW +R
Sbjct: 113 FLVEIFRLSLLHHPNLANLIGYCLDGDQRLLVHEFMPLGSLEDHLLDVVVGQQPLDWNSR 172

Query: 163 MKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVS 222
           +++ALGAAKGLEYLH+KANPPVIYRD K+SNILL+ DF+AKLSDFGLAKLG VGD  +VS
Sbjct: 173 IRIALGAAKGLEYLHEKANPPVIYRDFKSSNILLNVDFDAKLSDFGLAKLGSVGDTQNVS 232

Query: 223 SRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACP 282
           SRV+GTYGYCAPEY +TGQLT+KSDVYSFGVVLLELITGKRVID TRP ++QNLV WA P
Sbjct: 233 SRVVGTYGYCAPEYHKTGQLTVKSDVYSFGVVLLELITGKRVIDTTRPCHEQNLVTWAQP 292

Query: 283 IFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEA 342
           IF+EP +F +LADPLL  +FP KSLNQAVA+AAMCL EE  VRPLISDVVTALSF+  E 
Sbjct: 293 IFREPNRFPELADPLLQGEFPEKSLNQAVAIAAMCLQEEPIVRPLISDVVTALSFMSTET 352

Query: 343 THSSSLSLSSIDDPS-SVVSDQNLINQSKSND 374
              S L+ ++++  S   V DQ  +      D
Sbjct: 353 GSPSGLTGTALNPLSPKTVEDQGWLQCESPRD 384

BLAST of CmoCh08G000720 vs. ExPASy Swiss-Prot
Match: Q1PDV6 (Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 PE=1 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 2.8e-132
Identity = 233/329 (70.82%), Postives = 270/329 (82.07%), Query Frame = 0

Query: 13  SHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGF 72
           SH I   +  ++    Q +     + G   +IAA+TFTFRELA ATKNFR ECL+GEGGF
Sbjct: 35  SHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQTFTFRELAAATKNFRPECLLGEGGF 94

Query: 73  GRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRL 132
           GRVYKG+L+   Q+VAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRL
Sbjct: 95  GRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRL 154

Query: 133 LVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKAS 192
           LVYEYMPLGSLEDHL DLP D++ LDW+TRM +A GAAKGLEYLHDKANPPVIYRDLK+S
Sbjct: 155 LVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDLKSS 214

Query: 193 NILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFG 252
           NILL + ++ KLSDFGLAKLGPVGDK+HVS+RVMGTYGYCAPEY  TGQLTLKSDVYSFG
Sbjct: 215 NILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFG 274

Query: 253 VVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVA 312
           VV LELITG++ ID+ R   + NLVAWA P+FK+  KF  +ADP L   +P++ L QA+A
Sbjct: 275 VVFLELITGRKAIDNARAPGEHNLVAWARPLFKDRRKFPKMADPSLQGRYPMRGLYQALA 334

Query: 313 VAAMCLHEEATVRPLISDVVTALSFLGAE 342
           VAAMCL E+A  RPLI DVVTAL++L ++
Sbjct: 335 VAAMCLQEQAATRPLIGDVVTALTYLASQ 363

BLAST of CmoCh08G000720 vs. ExPASy Swiss-Prot
Match: Q0WRY5 (Probable serine/threonine-protein kinase PBL7 OS=Arabidopsis thaliana OX=3702 GN=PBL7 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 2.6e-130
Identity = 226/307 (73.62%), Postives = 260/307 (84.69%), Query Frame = 0

Query: 35  ENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKLDKISQVVAVKQLDR 94
           E++  G ++I A+TFTF ELATAT+NFR+ECLIGEGGFGRVYKG L   SQ  A+KQLD 
Sbjct: 47  ESKSKGSDHIVAQTFTFSELATATRNFRKECLIGEGGFGRVYKGYLASTSQTAAIKQLDH 106

Query: 95  NGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDLPADR 154
           NGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRLLVYEYMPLGSLEDHL D+   +
Sbjct: 107 NGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDISPGK 166

Query: 155 KALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLSDFGLAKLGP 214
           + LDW TRMK+A GAAKGLEYLHDK  PPVIYRDLK SNILLD+D+  KLSDFGLAKLGP
Sbjct: 167 QPLDWNTRMKIAAGAAKGLEYLHDKTMPPVIYRDLKCSNILLDDDYFPKLSDFGLAKLGP 226

Query: 215 VGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVIDDTRPANQQ 274
           VGDKSHVS+RVMGTYGYCAPEY  TGQLTLKSDVYSFGVVLLE+ITG++ ID +R   +Q
Sbjct: 227 VGDKSHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVLLEIITGRKAIDSSRSTGEQ 286

Query: 275 NLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMCLHEEATVRPLISDVVTA 334
           NLVAWA P+FK+  KF  +ADP+L   +P + L QA+AVAAMC+ E+  +RPLI+DVVTA
Sbjct: 287 NLVAWARPLFKDRRKFSQMADPMLQGQYPPRGLYQALAVAAMCVQEQPNLRPLIADVVTA 346

Query: 335 LSFLGAE 342
           LS+L ++
Sbjct: 347 LSYLASQ 353

BLAST of CmoCh08G000720 vs. ExPASy TrEMBL
Match: A0A6J1H9X8 (probable serine/threonine-protein kinase PBL25 OS=Cucurbita moschata OX=3662 GN=LOC111460950 PE=3 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.3e-214
Identity = 388/405 (95.80%), Postives = 391/405 (96.54%), Query Frame = 0

Query: 4   HVLNPTPLSSHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE 63
           H  +  P   H      ENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE
Sbjct: 29  HSRSLKPQQQHHPSPPSENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE 88

Query: 64  ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG 123
           ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG
Sbjct: 89  ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG 148

Query: 124 YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP 183
           YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP
Sbjct: 149 YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP 208

Query: 184 VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT 243
           VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT
Sbjct: 209 VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT 268

Query: 244 LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFP 303
           LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFP
Sbjct: 269 LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFP 328

Query: 304 VKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ 363
           VKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ
Sbjct: 329 VKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ 388

Query: 364 NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRRGTKTS 409
           NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRRG+ +S
Sbjct: 389 NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRRGSCSS 433

BLAST of CmoCh08G000720 vs. ExPASy TrEMBL
Match: A0A6J1KW86 (probable serine/threonine-protein kinase PBL25 OS=Cucurbita maxima OX=3661 GN=LOC111498138 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 9.9e-210
Identity = 378/388 (97.42%), Postives = 382/388 (98.45%), Query Frame = 0

Query: 21  ENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL 80
           EN KGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL
Sbjct: 48  ENPKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL 107

Query: 81  DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL 140
           DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL
Sbjct: 108 DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL 167

Query: 141 GSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDF 200
           GSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDF
Sbjct: 168 GSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDF 227

Query: 201 NAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELIT 260
           NAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELIT
Sbjct: 228 NAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELIT 287

Query: 261 GKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMCLHE 320
           GKRVIDDTRPANQQNLV WAC IFKEPTKFMDLADPLLGRDFPV+SLNQAVAVAAMCLHE
Sbjct: 288 GKRVIDDTRPANQQNLVTWACTIFKEPTKFMDLADPLLGRDFPVRSLNQAVAVAAMCLHE 347

Query: 321 EATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNDEEIAEAR 380
           EA VRPLISDVVTALSFLGAEATHSSSLSLSSIDDPS +VSDQNLINQSKSNDEEIAEAR
Sbjct: 348 EAMVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSLIVSDQNLINQSKSNDEEIAEAR 407

Query: 381 QRAVAEAIEWGSSSKQNAAASRRGTKTS 409
           QRAVAEAIEWGSSSKQNAAASRRG+ +S
Sbjct: 408 QRAVAEAIEWGSSSKQNAAASRRGSCSS 435

BLAST of CmoCh08G000720 vs. ExPASy TrEMBL
Match: A0A1S3C8C3 (serine/threonine-protein kinase At3g07070 OS=Cucumis melo OX=3656 GN=LOC103497640 PE=4 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.4e-179
Identity = 338/407 (83.05%), Postives = 354/407 (86.98%), Query Frame = 0

Query: 18  TIRENTKGNQVQNEA-----------EKENEIGGKNNIAAKTFTFRELATATKNFREECL 77
           T  EN K N VQ EA           EKE EIG KNNIAA+TFTFRELATATKNFR+ECL
Sbjct: 152 TPTENPKANPVQKEAIEKEKEKEKEKEKEKEIGEKNNIAAQTFTFRELATATKNFRQECL 211

Query: 78  IGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCA 137
           IGEGGFGRVYKGKLDK +QVVAVKQLDRNGLQGNREFLVEVLMLSLLHH+NLVNLIGYCA
Sbjct: 212 IGEGGFGRVYKGKLDKTNQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCA 271

Query: 138 DGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIY 197
           DGDQRLLVYEYMPLGSLEDHLLDLP +RKALDW TRMKV LGAAKGLEYLHDKANPPVIY
Sbjct: 272 DGDQRLLVYEYMPLGSLEDHLLDLPFERKALDWGTRMKVGLGAAKGLEYLHDKANPPVIY 331

Query: 198 RDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKS 257
           RDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT KS
Sbjct: 332 RDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKS 391

Query: 258 DVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKS 317
           DVYSFGVVLLELITGKRVID+TRPA QQNLVAWA PIFKEPT+F +LADPLLG DFPV+ 
Sbjct: 392 DVYSFGVVLLELITGKRVIDNTRPAKQQNLVAWAYPIFKEPTRFKELADPLLGGDFPVRG 451

Query: 318 LNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLI 377
           LNQAVAVAAMCLHEEAT RPLISDVVTALSFLGAE T SS  S S   D  S + DQ+LI
Sbjct: 452 LNQAVAVAAMCLHEEATARPLISDVVTALSFLGAETTQSSPSSSSG--DVPSPIDDQDLI 511

Query: 378 NQSKS-----NDEEIAEARQRAVAEAIEWGSSSKQNAAASRRGTKTS 409
           N S       +D+E AEARQRAVAEAIEWGS+SKQNAAASR G+ +S
Sbjct: 512 NLSNKPIDYFHDDETAEARQRAVAEAIEWGSTSKQNAAASRPGSHSS 556

BLAST of CmoCh08G000720 vs. ExPASy TrEMBL
Match: A0A0A0K554 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G338620 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 9.1e-179
Identity = 330/406 (81.28%), Postives = 355/406 (87.44%), Query Frame = 0

Query: 8   PTPLSSHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLI 67
           PTP  +     +++     + + E E+ENEIG KNNIAA+TFTFRELATATKNFR+ECLI
Sbjct: 59  PTPPENPKANPVQKEAVEKEKEKEKERENEIGEKNNIAAQTFTFRELATATKNFRQECLI 118

Query: 68  GEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCAD 127
           GEGGFGRVYKG+LDK +QVVAVKQLDRNGLQGNREFLVEVLMLSLLHH+NLVNLIGYCAD
Sbjct: 119 GEGGFGRVYKGRLDKTNQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCAD 178

Query: 128 GDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYR 187
           GDQRLLVYEYMPLGSLEDHLLDLP +RKALDW TRMKV LGAA+GLEYLHDKANPPVIYR
Sbjct: 179 GDQRLLVYEYMPLGSLEDHLLDLPFERKALDWGTRMKVGLGAAQGLEYLHDKANPPVIYR 238

Query: 188 DLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSD 247
           DLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT KSD
Sbjct: 239 DLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSD 298

Query: 248 VYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSL 307
           VYSFGVVLLELITGKRVID+TRPA QQNLVAWA PIFKEP +F +LADPLLG DFPV+ L
Sbjct: 299 VYSFGVVLLELITGKRVIDNTRPAKQQNLVAWAYPIFKEPARFKELADPLLGGDFPVRGL 358

Query: 308 NQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLIN 367
           NQAVAVAAMCLHEEAT RPLISDVVTALSFLGAE THSS  S S   D  S V DQ+LIN
Sbjct: 359 NQAVAVAAMCLHEEATARPLISDVVTALSFLGAETTHSSPSSSSG--DVPSPVDDQDLIN 418

Query: 368 QSKS-----NDEEIAEARQRAVAEAIEWGSSSKQNAAASRRGTKTS 409
            S       +D+E  EARQRAVAEAIEWG++SKQNAAASR G+ +S
Sbjct: 419 LSNKPVDYFHDDETDEARQRAVAEAIEWGATSKQNAAASRPGSYSS 462

BLAST of CmoCh08G000720 vs. ExPASy TrEMBL
Match: A0A6J1DCI7 (probable serine/threonine-protein kinase PBL25 OS=Momordica charantia OX=3673 GN=LOC111018838 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.5e-178
Identity = 331/403 (82.13%), Postives = 360/403 (89.33%), Query Frame = 0

Query: 18  TIRENTKGNQVQNEAE---------KENEIGGKNNIAAKTFTFRELATATKNFREECLIG 77
           T  EN KGNQ QN+AE         +E EI  KNNIAA+TFTFRELATATKNFR+ECLIG
Sbjct: 49  TPTENQKGNQQQNDAEREREKEKEGREREIXEKNNIAAQTFTFRELATATKNFRQECLIG 108

Query: 78  EGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADG 137
           EGGFGRVYKGKLDK +QVVAVKQLDRNGLQGNREFLVEVLMLSLLHH+NLVNLIGYCADG
Sbjct: 109 EGGFGRVYKGKLDKTAQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADG 168

Query: 138 DQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRD 197
           DQRLLVYEYMPLGSLEDHLLDLPA+++ALDW TRMKV LGAAKGLEYLHDKANPPVIYRD
Sbjct: 169 DQRLLVYEYMPLGSLEDHLLDLPAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRD 228

Query: 198 LKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDV 257
           LKASNILLD++FNAKLSDFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLTLKSDV
Sbjct: 229 LKASNILLDSEFNAKLSDFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDV 288

Query: 258 YSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLN 317
           YSFGVVLLELITGKRVID+TRP  QQNLVAWA PIFKEP++FM+LADPLLG +FPV++LN
Sbjct: 289 YSFGVVLLELITGKRVIDNTRPPKQQNLVAWAYPIFKEPSRFMELADPLLGGEFPVRALN 348

Query: 318 QAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQ 377
           QAVAVAAMCLHEEA+ RPL+SDVVTALSFLGAEA +SS    +S  D +S +S+ NLI+ 
Sbjct: 349 QAVAVAAMCLHEEASARPLMSDVVTALSFLGAEAVNSS----TSSSDITSPLSNHNLISL 408

Query: 378 SKSND---EEIAEARQRAVAEAIEWGSSSKQNAAASRRGTKTS 409
           SKSND   EEIAEARQRAVAEAIEWGS S+QN AASR G+ +S
Sbjct: 409 SKSNDDEKEEIAEARQRAVAEAIEWGSHSRQNVAASRPGSCSS 447

BLAST of CmoCh08G000720 vs. NCBI nr
Match: XP_022960099.1 (probable serine/threonine-protein kinase PBL25 [Cucurbita moschata])

HSP 1 Score: 754.6 bits (1947), Expect = 4.7e-214
Identity = 388/405 (95.80%), Postives = 391/405 (96.54%), Query Frame = 0

Query: 4   HVLNPTPLSSHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE 63
           H  +  P   H      ENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE
Sbjct: 29  HSRSLKPQQQHHPSPPSENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE 88

Query: 64  ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG 123
           ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG
Sbjct: 89  ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG 148

Query: 124 YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP 183
           YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP
Sbjct: 149 YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP 208

Query: 184 VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT 243
           VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT
Sbjct: 209 VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT 268

Query: 244 LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFP 303
           LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFP
Sbjct: 269 LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFP 328

Query: 304 VKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ 363
           VKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ
Sbjct: 329 VKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ 388

Query: 364 NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRRGTKTS 409
           NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRRG+ +S
Sbjct: 389 NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRRGSCSS 433

BLAST of CmoCh08G000720 vs. NCBI nr
Match: KAG6592868.1 (putative serine/threonine-protein kinase PBL26, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 746.5 bits (1926), Expect = 1.3e-211
Identity = 384/405 (94.81%), Postives = 388/405 (95.80%), Query Frame = 0

Query: 4   HVLNPTPLSSHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE 63
           H  +  P   H      ENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE
Sbjct: 29  HSRSLKPQQQHHPAPPSENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFRE 88

Query: 64  ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG 123
           ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG
Sbjct: 89  ECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIG 148

Query: 124 YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP 183
           YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP
Sbjct: 149 YCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPP 208

Query: 184 VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT 243
           VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT
Sbjct: 209 VIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLT 268

Query: 244 LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFP 303
           LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEP KFMDLADPLLGRDFP
Sbjct: 269 LKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPNKFMDLADPLLGRDFP 328

Query: 304 VKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ 363
           V+SLNQAVAVAAMCLHEEA VRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ
Sbjct: 329 VRSLNQAVAVAAMCLHEEAMVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQ 388

Query: 364 NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRRGTKTS 409
           NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASR G+ +S
Sbjct: 389 NLINQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAASRGGSCSS 433

BLAST of CmoCh08G000720 vs. NCBI nr
Match: XP_023515000.1 (probable serine/threonine-protein kinase PBL25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-209
Identity = 384/404 (95.05%), Postives = 389/404 (96.29%), Query Frame = 0

Query: 7   NPTPLSSHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECL 66
           +P P S H      +NTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECL
Sbjct: 41  HPAPPSGH---ICTDNTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECL 100

Query: 67  IGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCA 126
           IGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCA
Sbjct: 101 IGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCA 160

Query: 127 DGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIY 186
           DGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIY
Sbjct: 161 DGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIY 220

Query: 187 RDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKS 246
           RDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKS
Sbjct: 221 RDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKS 280

Query: 247 DVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKS 306
           DVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDF V+S
Sbjct: 281 DVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFTVRS 340

Query: 307 LNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLI 366
           LNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEA HSSSLSLSSIDDPS VVSDQNLI
Sbjct: 341 LNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEAMHSSSLSLSSIDDPSLVVSDQNLI 400

Query: 367 NQSKSNDEEIAEARQRAVAEAIEWGSSSKQN--AAASRRGTKTS 409
           NQSKSNDEEIAEARQRAVAEAIEWGSSSKQN  AAASRRG+ +S
Sbjct: 401 NQSKSNDEEIAEARQRAVAEAIEWGSSSKQNAAAAASRRGSCSS 441

BLAST of CmoCh08G000720 vs. NCBI nr
Match: XP_023005020.1 (probable serine/threonine-protein kinase PBL25 [Cucurbita maxima])

HSP 1 Score: 739.2 bits (1907), Expect = 2.0e-209
Identity = 378/388 (97.42%), Postives = 382/388 (98.45%), Query Frame = 0

Query: 21  ENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL 80
           EN KGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL
Sbjct: 48  ENPKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL 107

Query: 81  DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL 140
           DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL
Sbjct: 108 DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL 167

Query: 141 GSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDF 200
           GSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDF
Sbjct: 168 GSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDF 227

Query: 201 NAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELIT 260
           NAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELIT
Sbjct: 228 NAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELIT 287

Query: 261 GKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMCLHE 320
           GKRVIDDTRPANQQNLV WAC IFKEPTKFMDLADPLLGRDFPV+SLNQAVAVAAMCLHE
Sbjct: 288 GKRVIDDTRPANQQNLVTWACTIFKEPTKFMDLADPLLGRDFPVRSLNQAVAVAAMCLHE 347

Query: 321 EATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNDEEIAEAR 380
           EA VRPLISDVVTALSFLGAEATHSSSLSLSSIDDPS +VSDQNLINQSKSNDEEIAEAR
Sbjct: 348 EAMVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSLIVSDQNLINQSKSNDEEIAEAR 407

Query: 381 QRAVAEAIEWGSSSKQNAAASRRGTKTS 409
           QRAVAEAIEWGSSSKQNAAASRRG+ +S
Sbjct: 408 QRAVAEAIEWGSSSKQNAAASRRGSCSS 435

BLAST of CmoCh08G000720 vs. NCBI nr
Match: KAG7025274.1 (putative serine/threonine-protein kinase PBL26, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716.5 bits (1848), Expect = 1.4e-202
Identity = 381/451 (84.48%), Postives = 385/451 (85.37%), Query Frame = 0

Query: 21  ENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL 80
           ENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL
Sbjct: 45  ENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKL 104

Query: 81  DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL 140
           DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL
Sbjct: 105 DKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPL 164

Query: 141 GSLEDHLL---------------------------------------------------- 200
           GSLEDHLL                                                    
Sbjct: 165 GSLEDHLLEPVMGRCVNEDAGPNGVDCEIQAFSYKGVETSLLDAFYNCRQSLGGVPTRTL 224

Query: 201 -----------DLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLD 260
                      DLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLD
Sbjct: 225 APKRVDCEIPHDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLD 284

Query: 261 NDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLE 320
           NDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLE
Sbjct: 285 NDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLE 344

Query: 321 LITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMC 380
           LITGKRVIDDTRPANQQNLVAWACPIFKEP KFMDLADPLLGRDFPV+SLNQAVAVAAMC
Sbjct: 345 LITGKRVIDDTRPANQQNLVAWACPIFKEPNKFMDLADPLLGRDFPVRSLNQAVAVAAMC 404

Query: 381 LHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNDEEIA 409
           LHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSN+EEIA
Sbjct: 405 LHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNEEEIA 464

BLAST of CmoCh08G000720 vs. TAIR 10
Match: AT3G07070.1 (Protein kinase superfamily protein )

HSP 1 Score: 529.6 bits (1363), Expect = 2.3e-150
Identity = 275/381 (72.18%), Postives = 309/381 (81.10%), Query Frame = 0

Query: 18  TIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYK 77
           T+ E  K N    E          NNIAA+TF+FRELATATKNFR+ECLIGEGGFGRVYK
Sbjct: 44  TVNEQNKNNDEDKEV--------TNNIAAQTFSFRELATATKNFRQECLIGEGGFGRVYK 103

Query: 78  GKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEY 137
           GKL+K   +VAVKQLDRNGLQGN+EF+VEVLMLSLLHHK+LVNLIGYCADGDQRLLVYEY
Sbjct: 104 GKLEKTGMIVAVKQLDRNGLQGNKEFIVEVLMLSLLHHKHLVNLIGYCADGDQRLLVYEY 163

Query: 138 MPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLD 197
           M  GSLEDHLLDL  D+  LDW TR+++ALGAA GLEYLHDKANPPVIYRDLKA+NILLD
Sbjct: 164 MSRGSLEDHLLDLTPDQIPLDWDTRIRIALGAAMGLEYLHDKANPPVIYRDLKAANILLD 223

Query: 198 NDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLE 257
            +FNAKLSDFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLE
Sbjct: 224 GEFNAKLSDFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTTKSDVYSFGVVLLE 283

Query: 258 LITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMC 317
           LITG+RVID TRP ++QNLV WA P+FKEP++F +LADP L   FP K+LNQAVAVAAMC
Sbjct: 284 LITGRRVIDTTRPKDEQNLVTWAQPVFKEPSRFPELADPSLEGVFPEKALNQAVAVAAMC 343

Query: 318 LHEEATVRPLISDVVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNDEEIA 377
           L EEATVRPL+SDVVTAL FLG       S+S+   DDP    SD+  +      D   A
Sbjct: 344 LQEEATVRPLMSDVVTALGFLG--TAPDGSISVPHYDDPPQ-PSDETSV-----EDSVAA 403

Query: 378 EARQRAVAEAIEWGSSSKQNA 399
           E R+RAVAEA+EWG +S+ ++
Sbjct: 404 EERERAVAEAMEWGVASRAHS 408

BLAST of CmoCh08G000720 vs. TAIR 10
Match: AT3G24790.1 (Protein kinase superfamily protein )

HSP 1 Score: 508.4 bits (1308), Expect = 5.5e-144
Identity = 258/365 (70.68%), Postives = 298/365 (81.64%), Query Frame = 0

Query: 31  EAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKLDKISQVVAVK 90
           E   +N  G  NN+ A+ FTFRELATATKNFR+ECLIGEGGFGRVYKGKL+  +QVVAVK
Sbjct: 17  EVVAKNANGPSNNMGARIFTFRELATATKNFRQECLIGEGGFGRVYKGKLENPAQVVAVK 76

Query: 91  QLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDL 150
           QLDRNGLQG REFLVEVLMLSLLHH+NLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDL
Sbjct: 77  QLDRNGLQGQREFLVEVLMLSLLHHRNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDL 136

Query: 151 PADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLSDFGLA 210
              +K LDW TR+K+ALGAAKG+EYLHD+A+PPVIYRDLK+SNILLD ++ AKLSDFGLA
Sbjct: 137 EPGQKPLDWNTRIKIALGAAKGIEYLHDEADPPVIYRDLKSSNILLDPEYVAKLSDFGLA 196

Query: 211 KLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVIDDTRP 270
           KLGPVGD  HVSSRVMGTYGYCAPEYQRTG LT KSDVYSFGVVLLELI+G+RVID  RP
Sbjct: 197 KLGPVGDTLHVSSRVMGTYGYCAPEYQRTGYLTNKSDVYSFGVVLLELISGRRVIDTMRP 256

Query: 271 ANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMCLHEEATVRPLISD 330
           +++QNLV WA PIF++PT++  LADPLL  D+P KSLNQA+AVAAMCLHEE TVRPL+SD
Sbjct: 257 SHEQNLVTWALPIFRDPTRYWQLADPLLRGDYPEKSLNQAIAVAAMCLHEEPTVRPLMSD 316

Query: 331 VVTALSFLGAEATHSSSLSLSSIDDPSSVVSDQNLINQSKSNDEEIAEARQRAVAEAIEW 390
           V+TALSFLGA +  S++ S              N + Q++SN  +          +A++W
Sbjct: 317 VITALSFLGASSNSSNTGS--------------NHLQQNRSNKYQ----------DAVQW 357

Query: 391 GSSSK 396
            SS +
Sbjct: 377 DSSPR 357

BLAST of CmoCh08G000720 vs. TAIR 10
Match: AT4G13190.1 (Protein kinase superfamily protein )

HSP 1 Score: 476.9 bits (1226), Expect = 1.8e-134
Identity = 239/332 (71.99%), Postives = 276/332 (83.13%), Query Frame = 0

Query: 43  NIAAKTFTFRELATATKNFREECLIGEGGFGRVYKGKLDKISQVVAVKQLDRNGLQGNRE 102
           NI AK+F FRELATAT +FR+E LIGEGGFGRVYKGK++K  QVVAVKQLDRNGLQGNRE
Sbjct: 53  NIKAKSFKFRELATATNSFRQEFLIGEGGFGRVYKGKMEKTGQVVAVKQLDRNGLQGNRE 112

Query: 103 FLVEVLMLSLLHHKNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLDLPADRKALDWATR 162
           FLVE+  LSLLHH NL NLIGYC DGDQRLLV+E+MPLGSLEDHLLD+   ++ LDW +R
Sbjct: 113 FLVEIFRLSLLHHPNLANLIGYCLDGDQRLLVHEFMPLGSLEDHLLDVVVGQQPLDWNSR 172

Query: 163 MKVALGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLSDFGLAKLGPVGDKSHVS 222
           +++ALGAAKGLEYLH+KANPPVIYRD K+SNILL+ DF+AKLSDFGLAKLG VGD  +VS
Sbjct: 173 IRIALGAAKGLEYLHEKANPPVIYRDFKSSNILLNVDFDAKLSDFGLAKLGSVGDTQNVS 232

Query: 223 SRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVIDDTRPANQQNLVAWACP 282
           SRV+GTYGYCAPEY +TGQLT+KSDVYSFGVVLLELITGKRVID TRP ++QNLV WA P
Sbjct: 233 SRVVGTYGYCAPEYHKTGQLTVKSDVYSFGVVLLELITGKRVIDTTRPCHEQNLVTWAQP 292

Query: 283 IFKEPTKFMDLADPLLGRDFPVKSLNQAVAVAAMCLHEEATVRPLISDVVTALSFLGAEA 342
           IF+EP +F +LADPLL  +FP KSLNQAVA+AAMCL EE  VRPLISDVVTALSF+  E 
Sbjct: 293 IFREPNRFPELADPLLQGEFPEKSLNQAVAIAAMCLQEEPIVRPLISDVVTALSFMSTET 352

Query: 343 THSSSLSLSSIDDPS-SVVSDQNLINQSKSND 374
              S L+ ++++  S   V DQ  +      D
Sbjct: 353 GSPSGLTGTALNPLSPKTVEDQGWLQCESPRD 384

BLAST of CmoCh08G000720 vs. TAIR 10
Match: AT5G18610.1 (Protein kinase superfamily protein )

HSP 1 Score: 473.4 bits (1217), Expect = 2.0e-133
Identity = 233/329 (70.82%), Postives = 270/329 (82.07%), Query Frame = 0

Query: 13  SHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGF 72
           SH I   +  ++    Q +     + G   +IAA+TFTFRELA ATKNFR ECL+GEGGF
Sbjct: 35  SHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQTFTFRELAAATKNFRPECLLGEGGF 94

Query: 73  GRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRL 132
           GRVYKG+L+   Q+VAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRL
Sbjct: 95  GRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRL 154

Query: 133 LVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKAS 192
           LVYEYMPLGSLEDHL DLP D++ LDW+TRM +A GAAKGLEYLHDKANPPVIYRDLK+S
Sbjct: 155 LVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDLKSS 214

Query: 193 NILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFG 252
           NILL + ++ KLSDFGLAKLGPVGDK+HVS+RVMGTYGYCAPEY  TGQLTLKSDVYSFG
Sbjct: 215 NILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFG 274

Query: 253 VVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVA 312
           VV LELITG++ ID+ R   + NLVAWA P+FK+  KF  +ADP L   +P++ L QA+A
Sbjct: 275 VVFLELITGRKAIDNARAPGEHNLVAWARPLFKDRRKFPKMADPSLQGRYPMRGLYQALA 334

Query: 313 VAAMCLHEEATVRPLISDVVTALSFLGAE 342
           VAAMCL E+A  RPLI DVVTAL++L ++
Sbjct: 335 VAAMCLQEQAATRPLIGDVVTALTYLASQ 363

BLAST of CmoCh08G000720 vs. TAIR 10
Match: AT5G18610.2 (Protein kinase superfamily protein )

HSP 1 Score: 473.4 bits (1217), Expect = 2.0e-133
Identity = 233/329 (70.82%), Postives = 270/329 (82.07%), Query Frame = 0

Query: 13  SHDIGTIRENTKGNQVQNEAEKENEIGGKNNIAAKTFTFRELATATKNFREECLIGEGGF 72
           SH I   +  ++    Q +     + G   +IAA+TFTFRELA ATKNFR ECL+GEGGF
Sbjct: 35  SHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQTFTFRELAAATKNFRPECLLGEGGF 94

Query: 73  GRVYKGKLDKISQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHKNLVNLIGYCADGDQRL 132
           GRVYKG+L+   Q+VAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRL
Sbjct: 95  GRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRL 154

Query: 133 LVYEYMPLGSLEDHLLDLPADRKALDWATRMKVALGAAKGLEYLHDKANPPVIYRDLKAS 192
           LVYEYMPLGSLEDHL DLP D++ LDW+TRM +A GAAKGLEYLHDKANPPVIYRDLK+S
Sbjct: 155 LVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDLKSS 214

Query: 193 NILLDNDFNAKLSDFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFG 252
           NILL + ++ KLSDFGLAKLGPVGDK+HVS+RVMGTYGYCAPEY  TGQLTLKSDVYSFG
Sbjct: 215 NILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFG 274

Query: 253 VVLLELITGKRVIDDTRPANQQNLVAWACPIFKEPTKFMDLADPLLGRDFPVKSLNQAVA 312
           VV LELITG++ ID+ R   + NLVAWA P+FK+  KF  +ADP L   +P++ L QA+A
Sbjct: 275 VVFLELITGRKAIDNARAPGEHNLVAWARPLFKDRRKFPKMADPSLQGRYPMRGLYQALA 334

Query: 313 VAAMCLHEEATVRPLISDVVTALSFLGAE 342
           VAAMCL E+A  RPLI DVVTAL++L ++
Sbjct: 335 VAAMCLQEQAATRPLIGDVVTALTYLASQ 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SFT73.2e-14972.18Probable serine/threonine-protein kinase PBL26 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9LRY17.7e-14370.68Probable serine/threonine-protein kinase PBL25 OS=Arabidopsis thaliana OX=3702 G... [more]
Q1PE892.5e-13371.99Probable serine/threonine-protein kinase PBL24 OS=Arabidopsis thaliana OX=3702 G... [more]
Q1PDV62.8e-13270.82Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 P... [more]
Q0WRY52.6e-13073.62Probable serine/threonine-protein kinase PBL7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1H9X82.3e-21495.80probable serine/threonine-protein kinase PBL25 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KW869.9e-21097.42probable serine/threonine-protein kinase PBL25 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A1S3C8C31.4e-17983.05serine/threonine-protein kinase At3g07070 OS=Cucumis melo OX=3656 GN=LOC10349764... [more]
A0A0A0K5549.1e-17981.28Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G338... [more]
A0A6J1DCI74.5e-17882.13probable serine/threonine-protein kinase PBL25 OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
XP_022960099.14.7e-21495.80probable serine/threonine-protein kinase PBL25 [Cucurbita moschata][more]
KAG6592868.11.3e-21194.81putative serine/threonine-protein kinase PBL26, partial [Cucurbita argyrosperma ... [more]
XP_023515000.11.2e-20995.05probable serine/threonine-protein kinase PBL25 [Cucurbita pepo subsp. pepo][more]
XP_023005020.12.0e-20997.42probable serine/threonine-protein kinase PBL25 [Cucurbita maxima][more]
KAG7025274.11.4e-20284.48putative serine/threonine-protein kinase PBL26, partial [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
AT3G07070.12.3e-15072.18Protein kinase superfamily protein [more]
AT3G24790.15.5e-14470.68Protein kinase superfamily protein [more]
AT4G13190.11.8e-13471.99Protein kinase superfamily protein [more]
AT5G18610.12.0e-13370.82Protein kinase superfamily protein [more]
AT5G18610.22.0e-13370.82Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 61..338
e-value: 2.3E-43
score: 160.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 63..276
e-value: 4.2E-46
score: 157.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 61..338
score: 37.106709
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 27..137
e-value: 7.6E-34
score: 118.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 138..354
e-value: 2.7E-58
score: 198.7
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 58..262
e-value: 6.1E-12
score: 42.9
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 33..179
e-value: 8.9E-5
score: 18.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 393..412
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 393..413
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availablePANTHERPTHR47985:SF31SERINE/THREONINE-PROTEIN KINASE PBL26-RELATEDcoord: 23..399
NoneNo IPR availablePANTHERPTHR47985OS07G0668900 PROTEINcoord: 23..399
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 67..335
e-value: 1.40406E-98
score: 294.18
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 184..196
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 67..90
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 47..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G000720.1CmoCh08G000720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity