CmoCh08G000540 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G000540
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionChaperonin CPN60, mitochondrial
LocationCmo_Chr08: 333099 .. 337151 (+)
RNA-Seq ExpressionCmoCh08G000540
SyntenyCmoCh08G000540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCAGTTGTCTGCAGCCATTGCAGAGCTGAGCTAACTGAATTAACCTTTCCACTCGTTTCTTCTGCACCTTTCTCTTCTTTGCTCTTCTTCTCCATCTTCCCTGCAACTTCATTGCCACCATGCACCGCTTCGCCTGTGGCCTCGCCTCTAAAGCCAGGTACTTCACTACTTTTCACAACCTTCTTTCAATTGATTGTTTCTCATTCGTTTCTTTCTCATTTCCCTCCTCCTTTCTTCTTGAAGGTTGGCTAGGAACAGTGCCCATCAGGTATTTTACTCCGTATCTAATTCATTTTCTATAATGTGATCATGTTTTAATGCGCATTTTCGAGTAATGTCATGTGGTTACAATATATAATGTGAAGTTTGCTTCATTTTCATCAACTTCGACAGTTTTGTCAACTGGTATTGACAATTCTTTTTCCCGAATGAGGGATTTCAGATCAATTCTAGGTTGAACTGCAGTAGGAATTATGCAGCGAAGGACATTAAATTTGGGGTTGAAGCGCGCGCCTTGATGCTTAGGGGTGTTGAAGAGCTTGCAGATGCTGTCAAAGTTACAATGGGACCGAAAGTGAGTTGAACATGATCTAACTGTAAGACCTGTATGTATTTGTTGATTACTTTAGTGTTTAGTATATGATGATCACCAGTAGTAATCGACATATTAGGAGTGCACATGATCAGGCGTTCCATTTAGGTAGTTTACTGACTAAGTGTTCTTTCTCACTTGTTTTTAAAAGGGGCGTAATGTAGTGTTAGAGCAAAGTTATGGTGCCCCAAAAGTGACGAAAGATGGTGTTACTGTTGCAAAGAGCATTGAATTCAAAGATAAAGTAAAAAATATTGGTGCAAGTTTGGTGAAGCAGGTTGCAAATGCTACCAATGATGTGGCAGGAGATGGTGAGTAGTTGAAAACGTTCAAGAGGAGCGTGTTTTTCATGTGGTCTAAACAGAACAGGTGCAGTCACTGACATGAAGCTCCTTTCTCTTGTTTCAGGTACAACATGTGCAACGGTTCTTACTCGTGCAATATTTACTGAAGGATGTAAATCTGTGGCTGCTGGAGTGAACGCTATGGACTTGAGACGTGGGATTACTATGGCAGTTGATTCTGTTATTACAACTCTGAAGAGCAGAGCCCGGATGATCAGTACATCTGAAGAAATCGCCCAGGTTGGAACAATATCAGCTAATGGAGAACGAGAGATTGGTGAGCTAATAGCTAAGGCTATGGAAAAAGTAGGAAAGGAGGGAGTTATCACCATTTCAGTAAGTTATTTCAAACTCTCTCTTGTGTTCCACTATTTGTTTGTATAATACCTGGATGTCTTTAATAGCTTGTGGCTCTTTGGCTTACGGATTTGACTTAATCGACGTCTGAGTTCATAGTCCCGATTCATTTTCCGAGTCCTAAAATTCCTTTATGTCTAGCGTGAGAGCTATCTATCTCTTTCTCCATTTCTTTGCAGGAGGGTAACACACTCGATAATGAATTGGAGATCGTTGAGGGAATGAAGCTGGACAGGGGTTTCATATCTCCTTATTTTATTACCAACCAGAAAAACCAAAAATGTGTAAGGATTTAGTCACTTGGTTATTTTTTTTGTGATTCATCATGGTGTGGTTTTGTCTCGTCTTCCGATTCATGTTCATATTCATATATTAACTTCGGGACTGGGTTCGGGGACTTGACATTTAGCTTTCGATAATGACCTTTACAGATTTTCACATCTTATTCAGCGTTATAAATTATGATAGACTTCTCGATTGTTTCGTCTTACAGGAATTGGAAGATCCACTAATTTTAATACACGAAAAGAAAATCTCAAATTTAAATGCTGTGGTAAAAGTCTTGGAGTTAGCTCTGAAGGTAACCTATCCTTTTTAGTGGATGTTGACTGTGAGTTTAGTCTGAATATCACTGCTTAACAGTATGTCTTCCATTTTCACCTTAGAGGCAAAGACCTTTACTTATTGTTGCTGAAGATGTGGAAAGTGATGCATTAGCTACCCTTATTTTAAACAAGTTACGTGCTGGTATTAAGGTAACATAAATGTTCTTAGTTACATGCTAAATTTTAGTGGTAACAATGGATATACCAACTAAAATGGTGTTTATCACTTAAACGAACGTAAGGTTTGCGCAATTAAAGCTCCCGGGTTCGGAGATAATAGGAAGGCTAGTATGCAGGATTTGGCAATTCTCACAGGAGGCCAGGTAGACTTTCTGACAATTTGCATCTCTTCCACCATGTTTTTCTTGAAGATCTCATATTCAATTATACTTTTTGTTTCTTTTTACGTTGAATATCAGGTCATAACTGAGGAACTTGGTCTAGATCTTGAAAAAGTTGGTGCTGAATCATTGGGTTCATGCAAAAAGGTGAAATAATGTTACTTTTTTGCCACATCGTGATTATATTTCTAACATAATCAATGTTGTGCATATCGGCATAGGTTACGGTGTCGAAGGATGATACTGTAGTTCTTGATGGGGCTGGTGACAAGAAGGCGATTGAAGAACAGTCTGACCAGGTTTCATTTTCTTCATTGAATGTCATGTTATGTTTTGAATTTCGTTTGGTTCCTTGGTGCGTGAGTATACGAATAATTTCATGATCGAACGTGTGTGATAGTTGCGATCCTTGATTGAGTTGAGCACTTCAGACTATGACAAGGAAAAGCTGCAAGAACGGCTTGCAAAGCTTTCTGGCGGAGTTGCTGTTCTAAAGGTAAGTGTTTATGTCATGTAACTCAACTCGTTAAGGCATAAACATGCCAGCACTCCGCTTGATTCGAGCTCTCGACTCACGTTTTTTTCTAGATTGGTGGGGCTAGTGAAGCCGAAGTTGGGGAACGAAAGGATAGAGTCACCGATGCGCTAAATGCTACCAAAGCGGCCGTGGAGGAGGGAATTGTTGCTGGTAATGCCATCTTCTGAGATTATCTTTTTGGTTGGGATGGCTGAGAATTTGAAATGATAAATAATGTTCTATAACTTCATTCTTCATATCTTGTTGATGGGAGATTCGAAACCATCAGCATTATGATTACATCCAATTCGAAAAAACGAACTTGATATCTCGAGAGTTGGCATGGCAGGCGGTGGTGTCGCTCTGCTCTATGCATCAAAGGAGTTGGAGAAGTTGCATACTGCAAATTTTGATCAGAAGATTGGCGTTCAAATCATTCAAAACGCTCTAAAGGTTTCGAAATTTTTCATCCATATATTGAAACCACTATGCATTATGGATATGTTACAATCATATTGTACAAGTATCAGTTAACTTAAGGCTGCTTCATCTGCAGATGCCTATTTACACAATTGCTTCTAATGCCGGAGTTGAAGGAGCAGTTGTGGCCGGGAAGCTACTCGAACAGGACAATTCCGACCTTGGATACGACGCAGCTAAAGGTCTGTATATTACGCCCCACGACATACAAAATGAATAGCAAGGTTTCGTTTTGTTTGTTGCCAGCCAAAGTTCTTGCAACTTCTGCTTATATAGTCTCCTACATTTCTTCATTTAGGCGAATATGTAGACATGATCAAGGCTGGAATTATTGATCCCTTGAAAGTTATCAGAACCGCGCTAGTAGACGCCGCAAGGTAGCTTCGCTAGCCTCGCTCGAGATGCATTGTCTCTTAATGTTGTTATTATTTAAGCTTGGCTTGGCCCTTACAAGCTTTGCTATTGTCACAGTGTTTCTTCGCTACTGACGACGACGGAGGCCGTGGTGGCCGAACAAGCTAAGGATGATAAGGAAGCTGCCCCATCAATGGGAGGCGCCATGGGTTACTGAAACCGAACCCACCCCGATTTTGTTAACAATTGATCATTCATTTGCAGACCAATTCATAAGACATGTTTTTTCGACGACTTATTGCATCAATGGTTAGGTTGAGCCGAATTCATCGAGTTGAAATTTTTAGAAAAACGTCTTTCGAATCTAATAATCCAAGTCAATCTGAGGAACATGATGTGGACATCAGTCAGCCCAATCATCATCTGAAAAGCAGATGACACGGTATCAGGTGGAGGGTGAGG

mRNA sequence

TGCAGTTGTCTGCAGCCATTGCAGAGCTGAGCTAACTGAATTAACCTTTCCACTCGTTTCTTCTGCACCTTTCTCTTCTTTGCTCTTCTTCTCCATCTTCCCTGCAACTTCATTGCCACCATGCACCGCTTCGCCTGTGGCCTCGCCTCTAAAGCCAGGTTGGCTAGGAACAGTGCCCATCAGATCAATTCTAGGTTGAACTGCAGTAGGAATTATGCAGCGAAGGACATTAAATTTGGGGTTGAAGCGCGCGCCTTGATGCTTAGGGGTGTTGAAGAGCTTGCAGATGCTGTCAAAGTTACAATGGGACCGAAAGGGCGTAATGTAGTGTTAGAGCAAAGTTATGGTGCCCCAAAAGTGACGAAAGATGGTGTTACTGTTGCAAAGAGCATTGAATTCAAAGATAAAGTAAAAAATATTGGTGCAAGTTTGGTGAAGCAGGTTGCAAATGCTACCAATGATGTGGCAGGAGATGGTACAACATGTGCAACGGTTCTTACTCGTGCAATATTTACTGAAGGATGTAAATCTGTGGCTGCTGGAGTGAACGCTATGGACTTGAGACGTGGGATTACTATGGCAGTTGATTCTGTTATTACAACTCTGAAGAGCAGAGCCCGGATGATCAGTACATCTGAAGAAATCGCCCAGGTTGGAACAATATCAGCTAATGGAGAACGAGAGATTGGTGAGCTAATAGCTAAGGCTATGGAAAAAGTAGGAAAGGAGGGAGTTATCACCATTTCAGAGGGTAACACACTCGATAATGAATTGGAGATCGTTGAGGGAATGAAGCTGGACAGGGGTTTCATATCTCCTTATTTTATTACCAACCAGAAAAACCAAAAATGTGAATTGGAAGATCCACTAATTTTAATACACGAAAAGAAAATCTCAAATTTAAATGCTGTGGTAAAAGTCTTGGAGTTAGCTCTGAAGAGGCAAAGACCTTTACTTATTGTTGCTGAAGATGTGGAAAGTGATGCATTAGCTACCCTTATTTTAAACAAGTTACGTGCTGGTATTAAGGTTTGCGCAATTAAAGCTCCCGGGTTCGGAGATAATAGGAAGGCTAGTATGCAGGATTTGGCAATTCTCACAGGAGGCCAGGTCATAACTGAGGAACTTGGTCTAGATCTTGAAAAAGTTGGTGCTGAATCATTGGGTTCATGCAAAAAGGTTACGGTGTCGAAGGATGATACTGTAGTTCTTGATGGGGCTGGTGACAAGAAGGCGATTGAAGAACAGTCTGACCAGTTGCGATCCTTGATTGAGTTGAGCACTTCAGACTATGACAAGGAAAAGCTGCAAGAACGGCTTGCAAAGCTTTCTGGCGGAGTTGCTGTTCTAAAGATTGGTGGGGCTAGTGAAGCCGAAGTTGGGGAACGAAAGGATAGAGTCACCGATGCGCTAAATGCTACCAAAGCGGCCGTGGAGGAGGGAATTGTTGCTGGCGGTGGTGTCGCTCTGCTCTATGCATCAAAGGAGTTGGAGAAGTTGCATACTGCAAATTTTGATCAGAAGATTGGCGTTCAAATCATTCAAAACGCTCTAAAGATGCCTATTTACACAATTGCTTCTAATGCCGGAGTTGAAGGAGCAGTTGTGGCCGGGAAGCTACTCGAACAGGACAATTCCGACCTTGGATACGACGCAGCTAAAGGCGAATATGTAGACATGATCAAGGCTGGAATTATTGATCCCTTGAAAGTTATCAGAACCGCGCTAGTAGACGCCGCAAGTGTTTCTTCGCTACTGACGACGACGGAGGCCGTGGTGGCCGAACAAGCTAAGGATGATAAGGAAGCTGCCCCATCAATGGGAGGCGCCATGGGTTACTGAAACCGAACCCACCCCGATTTTGTTAACAATTGATCATTCATTTGCAGACCAATTCATAAGACATGTTTTTTCGACGACTTATTGCATCAATGGTTAGGTTGAGCCGAATTCATCGAGTTGAAATTTTTAGAAAAACGTCTTTCGAATCTAATAATCCAAGTCAATCTGAGGAACATGATGTGGACATCAGTCAGCCCAATCATCATCTGAAAAGCAGATGACACGGTATCAGGTGGAGGGTGAGG

Coding sequence (CDS)

ATGCACCGCTTCGCCTGTGGCCTCGCCTCTAAAGCCAGGTTGGCTAGGAACAGTGCCCATCAGATCAATTCTAGGTTGAACTGCAGTAGGAATTATGCAGCGAAGGACATTAAATTTGGGGTTGAAGCGCGCGCCTTGATGCTTAGGGGTGTTGAAGAGCTTGCAGATGCTGTCAAAGTTACAATGGGACCGAAAGGGCGTAATGTAGTGTTAGAGCAAAGTTATGGTGCCCCAAAAGTGACGAAAGATGGTGTTACTGTTGCAAAGAGCATTGAATTCAAAGATAAAGTAAAAAATATTGGTGCAAGTTTGGTGAAGCAGGTTGCAAATGCTACCAATGATGTGGCAGGAGATGGTACAACATGTGCAACGGTTCTTACTCGTGCAATATTTACTGAAGGATGTAAATCTGTGGCTGCTGGAGTGAACGCTATGGACTTGAGACGTGGGATTACTATGGCAGTTGATTCTGTTATTACAACTCTGAAGAGCAGAGCCCGGATGATCAGTACATCTGAAGAAATCGCCCAGGTTGGAACAATATCAGCTAATGGAGAACGAGAGATTGGTGAGCTAATAGCTAAGGCTATGGAAAAAGTAGGAAAGGAGGGAGTTATCACCATTTCAGAGGGTAACACACTCGATAATGAATTGGAGATCGTTGAGGGAATGAAGCTGGACAGGGGTTTCATATCTCCTTATTTTATTACCAACCAGAAAAACCAAAAATGTGAATTGGAAGATCCACTAATTTTAATACACGAAAAGAAAATCTCAAATTTAAATGCTGTGGTAAAAGTCTTGGAGTTAGCTCTGAAGAGGCAAAGACCTTTACTTATTGTTGCTGAAGATGTGGAAAGTGATGCATTAGCTACCCTTATTTTAAACAAGTTACGTGCTGGTATTAAGGTTTGCGCAATTAAAGCTCCCGGGTTCGGAGATAATAGGAAGGCTAGTATGCAGGATTTGGCAATTCTCACAGGAGGCCAGGTCATAACTGAGGAACTTGGTCTAGATCTTGAAAAAGTTGGTGCTGAATCATTGGGTTCATGCAAAAAGGTTACGGTGTCGAAGGATGATACTGTAGTTCTTGATGGGGCTGGTGACAAGAAGGCGATTGAAGAACAGTCTGACCAGTTGCGATCCTTGATTGAGTTGAGCACTTCAGACTATGACAAGGAAAAGCTGCAAGAACGGCTTGCAAAGCTTTCTGGCGGAGTTGCTGTTCTAAAGATTGGTGGGGCTAGTGAAGCCGAAGTTGGGGAACGAAAGGATAGAGTCACCGATGCGCTAAATGCTACCAAAGCGGCCGTGGAGGAGGGAATTGTTGCTGGCGGTGGTGTCGCTCTGCTCTATGCATCAAAGGAGTTGGAGAAGTTGCATACTGCAAATTTTGATCAGAAGATTGGCGTTCAAATCATTCAAAACGCTCTAAAGATGCCTATTTACACAATTGCTTCTAATGCCGGAGTTGAAGGAGCAGTTGTGGCCGGGAAGCTACTCGAACAGGACAATTCCGACCTTGGATACGACGCAGCTAAAGGCGAATATGTAGACATGATCAAGGCTGGAATTATTGATCCCTTGAAAGTTATCAGAACCGCGCTAGTAGACGCCGCAAGTGTTTCTTCGCTACTGACGACGACGGAGGCCGTGGTGGCCGAACAAGCTAAGGATGATAAGGAAGCTGCCCCATCAATGGGAGGCGCCATGGGTTACTGA

Protein sequence

MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKVTMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGTTCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGTISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQKNQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRAGIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDDTVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEVGERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKMPIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAASVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY
Homology
BLAST of CmoCh08G000540 vs. ExPASy Swiss-Prot
Match: Q05046 (Chaperonin CPN60-2, mitochondrial OS=Cucurbita maxima OX=3661 GN=CPN60-2 PE=1 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 1.3e-275
Identity = 503/572 (87.94%), Postives = 544/572 (95.10%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFA GLASKARLAR  A+QI SR + SRNYAAKD+KFGVEAR LML+GVE+LADAVKV
Sbjct: 1   MHRFASGLASKARLARKGANQIASRSSWSRNYAAKDVKFGVEARGLMLKGVEDLADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVV+EQSYGAPKVTKDGVTVAKSIEFKDKVKN+GASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVIEQSYGAPKVTKDGVTVAKSIEFKDKVKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCAT+LTRAIFTEGCKSVAAG+NAMDLRRGI+MAVDSV+T LKSRARMISTSEEIAQVGT
Sbjct: 121 TCATILTRAIFTEGCKSVAAGMNAMDLRRGISMAVDSVVTNLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+G TL NELE+VEGMKLDRG+ISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGKTLFNELEVVEGMKLDRGYISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCEL+DPLILIHEKKIS++N+VVKVLELALKRQRPLLIV+EDVESDALATLILNKLRA
Sbjct: 241 NQKCELDDPLILIHEKKISSINSVVKVLELALKRQRPLLIVSEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFG+NRKA + DLA+LTGGQ+ITEELG++LEKV  + LGSCKK+T+SKDD
Sbjct: 301 GIKVCAIKAPGFGENRKAGLHDLAVLTGGQLITEELGMNLEKVDLDMLGSCKKITISKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TV+LDGAGDKK+IEE+ +Q+RS IELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVILDGAGDKKSIEERCEQIRSAIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GE+KDRVTDALNATKAAVEEGIV GGGVALLYASKEL+KL TANFDQKIGVQIIQNALK 
Sbjct: 421 GEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELDKLSTANFDQKIGVQIIQNALKT 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           P++TIASNAGVEGAVV GKLLEQDN DLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PVHTIASNAGVEGAVVVGKLLEQDNPDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMG 573
           SVSSL+TTTEA+V E  KD+KE  P+MGG MG
Sbjct: 541 SVSSLMTTTEAIVVELPKDEKE-VPAMGGGMG 571

BLAST of CmoCh08G000540 vs. ExPASy Swiss-Prot
Match: Q05045 (Chaperonin CPN60-1, mitochondrial OS=Cucurbita maxima OX=3661 GN=CPN60-1 PE=1 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 6.2e-273
Identity = 497/572 (86.89%), Postives = 542/572 (94.76%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFA GLASKARLARN A+QI SR N  RNYAAKD+KFGVEAR LML+GVE+LADAVKV
Sbjct: 1   MHRFATGLASKARLARNGANQIASRSNWRRNYAAKDVKFGVEARGLMLKGVEDLADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGR VV+EQS+GAPKVTKDGVTVAKSIEFKDKVKN+GASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRTVVIEQSFGAPKVTKDGVTVAKSIEFKDKVKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCAT+LT+AIFTEGCKSVA+G+NAMDLRRGI+MAVDSV+T LKSRARMISTSEEIAQVGT
Sbjct: 121 TCATILTKAIFTEGCKSVASGMNAMDLRRGISMAVDSVVTNLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+G T+DNELE+VEGMKLDRG+ISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGKTMDNELEVVEGMKLDRGYISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCEL+DPLI+I+EKKIS++NAVVKVLELALK+QRPLLIV+EDVES+ALATLILNKLRA
Sbjct: 241 NQKCELDDPLIIIYEKKISSINAVVKVLELALKKQRPLLIVSEDVESEALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFG+NRKA +QDLA+LTGGQVITEELG++LEKV  + LGSCKK+T+SKDD
Sbjct: 301 GIKVCAIKAPGFGENRKAGLQDLAVLTGGQVITEELGMNLEKVDLDMLGSCKKITISKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TV+LDGAGDKKAIEE+ DQ+RS IE STSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVILDGAGDKKAIEERCDQIRSGIEASTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GE+KDRVTDALNATKAAVEEGIV GGGVALLYASKEL+KL TANFDQKIGVQIIQNALK 
Sbjct: 421 GEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELDKLPTANFDQKIGVQIIQNALKT 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           P++TIASNAGVEGAVV GKLLEQD+ DLGYDAAKGEYVDM+KAGIIDPLKVIRTALVDAA
Sbjct: 481 PVHTIASNAGVEGAVVVGKLLEQDDPDLGYDAAKGEYVDMVKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMG 573
           SVSSL+TTTE VV E  KD+ E  P+MGG MG
Sbjct: 541 SVSSLMTTTEVVVVELPKDENE-VPAMGGGMG 571

BLAST of CmoCh08G000540 vs. ExPASy Swiss-Prot
Match: P29197 (Chaperonin CPN60, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CPN60 PE=1 SV=2)

HSP 1 Score: 919.1 bits (2374), Expect = 2.5e-266
Identity = 489/575 (85.04%), Postives = 538/575 (93.57%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           M+RFA  LASKAR+A+N A Q++SR++ SRNYAAK+IKFGVEARALML+GVE+LADAVKV
Sbjct: 1   MYRFASNLASKARIAQN-ARQVSSRMSWSRNYAAKEIKFGVEARALMLKGVEDLADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVV+EQS+GAPKVTKDGVTVAKSIEFKDK+KN+GASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVIEQSWGAPKVTKDGVTVAKSIEFKDKIKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIF EGCKSVAAG+NAMDLRRGI+MAVD+V+T LKS+ARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFAEGCKSVAAGMNAMDLRRGISMAVDAVVTNLKSKARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITI +G TL NELE+VEGMKLDRG+ SPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITIQDGKTLFNELEVVEGMKLDRGYTSPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
            QKCEL+DPLILIHEKKIS++N++VKVLELALKRQRPLLIV+EDVESDALATLILNKLRA
Sbjct: 241 TQKCELDDPLILIHEKKISSINSIVKVLELALKRQRPLLIVSEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFG+NRKA++QDLA LTGG+VIT+ELG++LEKV    LG+CKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGENRKANLQDLAALTGGEVITDELGMNLEKVDLSMLGTCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TV+LDGAGDKK IEE+ +Q+RS IELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVILDGAGDKKGIEERCEQIRSAIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GE+KDRVTDALNATKAAVEEGI+ GGGVALLYA++ELEKL TANFDQKIGVQIIQNALK 
Sbjct: 421 GEKKDRVTDALNATKAAVEEGILPGGGVALLYAARELEKLPTANFDQKIGVQIIQNALKT 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           P+YTIASNAGVEGAV+ GKLLEQDN DLGYDAAKGEYVDM+KAGIIDPLKVIRTALVDAA
Sbjct: 481 PVYTIASNAGVEGAVIVGKLLEQDNPDLGYDAAKGEYVDMVKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKE---AAPSMGGAMG 573
           SVSSLLTTTEAVV +  KD+ E   A   MGG  G
Sbjct: 541 SVSSLLTTTEAVVVDLPKDESESGAAGAGMGGMGG 574

BLAST of CmoCh08G000540 vs. ExPASy Swiss-Prot
Match: P29185 (Chaperonin CPN60-1, mitochondrial OS=Zea mays OX=4577 GN=CPN60I PE=1 SV=2)

HSP 1 Score: 907.1 bits (2343), Expect = 9.9e-263
Identity = 481/574 (83.80%), Postives = 534/574 (93.03%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNS--AHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAV 60
           M+R A  LASKAR A NS    Q+ SRL  SRNYAAKDIKFGVEARALMLRGVEELADAV
Sbjct: 1   MYRAAASLASKARQAGNSLATRQVGSRLAWSRNYAAKDIKFGVEARALMLRGVEELADAV 60

Query: 61  KVTMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGD 120
           KVTMGPKGRNVV+EQS+GAPKVTKDGVTVAKSIEFKD+VKN+GASLVKQVANATND AGD
Sbjct: 61  KVTMGPKGRNVVIEQSFGAPKVTKDGVTVAKSIEFKDRVKNVGASLVKQVANATNDTAGD 120

Query: 121 GTTCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQV 180
           GTTCATVLT+AIFTEGCKSVAAG+NAMDLRRGI+MAVD+V+T LK  ARMISTSEEIAQV
Sbjct: 121 GTTCATVLTKAIFTEGCKSVAAGMNAMDLRRGISMAVDAVVTNLKGMARMISTSEEIAQV 180

Query: 181 GTISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITN 240
           GTISANGEREIGELIAKAMEKVGKEGVITI++GNTL NELE+VEGMKLDRG+ISPYFITN
Sbjct: 181 GTISANGEREIGELIAKAMEKVGKEGVITIADGNTLYNELEVVEGMKLDRGYISPYFITN 240

Query: 241 QKNQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKL 300
            K QKCELEDPLILIH+KK++N++AVVKVLE+ALK+Q+PLLIVAEDVES+AL TLI+NKL
Sbjct: 241 SKTQKCELEDPLILIHDKKVTNMHAVVKVLEMALKKQKPLLIVAEDVESEALGTLIINKL 300

Query: 301 RAGIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSK 360
           RAGIKVCA+KAPGFG+NRKA++QDLAILTGG+VITEELG++LE      LG+CKKVTVSK
Sbjct: 301 RAGIKVCAVKAPGFGENRKANLQDLAILTGGEVITEELGMNLENFEPHMLGTCKKVTVSK 360

Query: 361 DDTVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEA 420
           DDTV+LDGAGDKK+IEE+++Q+RS IE STSDYDKEKLQERLAKLSGGVAVLKIGGASEA
Sbjct: 361 DDTVILDGAGDKKSIEERAEQIRSAIENSTSDYDKEKLQERLAKLSGGVAVLKIGGASEA 420

Query: 421 EVGERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNAL 480
           EVGE+KDRVTDALNATKAAVEEGIV GGGVALLYASKEL+KL TANFDQKIGVQIIQNAL
Sbjct: 421 EVGEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELDKLQTANFDQKIGVQIIQNAL 480

Query: 481 KMPIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVD 540
           K P++TIASNAGVEGAVV GKLLEQ+N+DLGYDAAKGEYVDM+K GIIDPLKVIRTALVD
Sbjct: 481 KTPVHTIASNAGVEGAVVVGKLLEQENTDLGYDAAKGEYVDMVKTGIIDPLKVIRTALVD 540

Query: 541 AASVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMG 573
           AASVSSL+TTTE+++ E  K++   AP+MGG MG
Sbjct: 541 AASVSSLMTTTESIIVEIPKEE-APAPAMGGGMG 573

BLAST of CmoCh08G000540 vs. ExPASy Swiss-Prot
Match: Q43298 (Chaperonin CPN60-2, mitochondrial OS=Zea mays OX=4577 GN=CPN60II PE=2 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 2.9e-262
Identity = 483/574 (84.15%), Postives = 534/574 (93.03%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNS--AHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAV 60
           M+R A  LASKAR A +S  A Q+ SRL  SRNYAAKDIKFGVEARALMLRGVEELADAV
Sbjct: 1   MYRAAASLASKARQAGSSSAARQVGSRLAWSRNYAAKDIKFGVEARALMLRGVEELADAV 60

Query: 61  KVTMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGD 120
           KVTMGPKGRNVV+EQS+GAPKVTKDGVTVAKSIEFKD+VKN+GASLVKQVANATND AGD
Sbjct: 61  KVTMGPKGRNVVIEQSFGAPKVTKDGVTVAKSIEFKDRVKNVGASLVKQVANATNDTAGD 120

Query: 121 GTTCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQV 180
           GTTCATVLT+AIFTEGCKSVAAG+NAMDLRRGI+MAVD+V+T LK  ARMISTSEEIAQV
Sbjct: 121 GTTCATVLTKAIFTEGCKSVAAGMNAMDLRRGISMAVDAVVTNLKGMARMISTSEEIAQV 180

Query: 181 GTISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITN 240
           GTISANGEREIGELIAKAMEKVGKEGVITI++GNTL NELE+VEGMKLDRG+ISPYFITN
Sbjct: 181 GTISANGEREIGELIAKAMEKVGKEGVITIADGNTLYNELEVVEGMKLDRGYISPYFITN 240

Query: 241 QKNQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKL 300
            K QKCELEDPLILIH+KK++N++AVVKVLE+ALK+QRPLLIVAEDVES+AL TLI+NKL
Sbjct: 241 SKAQKCELEDPLILIHDKKVTNMHAVVKVLEMALKKQRPLLIVAEDVESEALGTLIINKL 300

Query: 301 RAGIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSK 360
           RAGIKVCA+KAPGFG+NRKA++QDLAILTGG+VITEELG++LE V    LGSCKKVTVSK
Sbjct: 301 RAGIKVCAVKAPGFGENRKANLQDLAILTGGEVITEELGMNLENVEPHMLGSCKKVTVSK 360

Query: 361 DDTVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEA 420
           DDTV+LDGAGDKK+IEE++DQ+RS +E STSDYDKEKLQERLAKLSGGVAVLKIGGASEA
Sbjct: 361 DDTVILDGAGDKKSIEERADQIRSAVENSTSDYDKEKLQERLAKLSGGVAVLKIGGASEA 420

Query: 421 EVGERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNAL 480
           EVGE+KDRVTDALNATKAAVEEGIV GGGVALLYASKEL+KL TANFDQKIGVQIIQNAL
Sbjct: 421 EVGEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELDKLQTANFDQKIGVQIIQNAL 480

Query: 481 KMPIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVD 540
           K P++TIASNAGVEGAVV GKLLEQ N+DLGYDAAK EYVDM+KAGIIDPLKVIRTALVD
Sbjct: 481 KTPVHTIASNAGVEGAVVVGKLLEQGNTDLGYDAAKDEYVDMVKAGIIDPLKVIRTALVD 540

Query: 541 AASVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMG 573
           AASVSSL+TTTE+++ E  K++   AP+MGG  G
Sbjct: 541 AASVSSLMTTTESIIVEIPKEE-APAPAMGGMGG 573

BLAST of CmoCh08G000540 vs. ExPASy TrEMBL
Match: A0A6J1H4V2 (chaperonin CPN60-2, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111460590 PE=3 SV=1)

HSP 1 Score: 1063.5 bits (2749), Expect = 3.0e-307
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV
Sbjct: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA
Sbjct: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM
Sbjct: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY
Sbjct: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 573

BLAST of CmoCh08G000540 vs. ExPASy TrEMBL
Match: A0A6J1L0Z8 (chaperonin CPN60-2, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111498113 PE=3 SV=1)

HSP 1 Score: 1048.1 bits (2709), Expect = 1.3e-302
Identity = 564/573 (98.43%), Postives = 567/573 (98.95%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFACGLASKARLARNSAH INSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV
Sbjct: 1   MHRFACGLASKARLARNSAHLINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+GNTLDNELEIVEGMK+DRGFISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGNTLDNELEIVEGMKMDRGFISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVES+ALATLILNKLRA
Sbjct: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESEALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKA MQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKAGMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDG GDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGTGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELE LHTANFDQKIGVQIIQNALKM
Sbjct: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELENLHTANFDQKIGVQIIQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSLLTTTEAVV EQAKD KEAAPSMGGAMGY
Sbjct: 541 SVSSLLTTTEAVVVEQAKDGKEAAPSMGGAMGY 573

BLAST of CmoCh08G000540 vs. ExPASy TrEMBL
Match: A0A6J1DCE4 (chaperonin CPN60-2, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111018791 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 6.6e-294
Identity = 548/573 (95.64%), Postives = 563/573 (98.25%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFACGLASKARLA+N+AHQINSRLN SRNYAAKDIKFGVEARA MLRGVEELADAVKV
Sbjct: 1   MHRFACGLASKARLAKNTAHQINSRLNWSRNYAAKDIKFGVEARASMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGA LVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGAGLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMA+DSV++TLKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAIDSVVSTLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+GNTLDNELEIVEGMKLDRG+ISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGNTLDNELEIVEGMKLDRGYISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDP+ILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVES+ALATLILNKLRA
Sbjct: 241 NQKCELEDPIILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESEALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKA++QDLAILTGGQVITEELGLDLEKVG ESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKANLQDLAILTGGQVITEELGLDLEKVGFESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDKKAIEE+SDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGAGDKKAIEERSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GE+KDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKL T NFDQKIGVQIIQNALKM
Sbjct: 421 GEKKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLQTPNFDQKIGVQIIQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGAVV GKLLEQ+NSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAVVIGKLLEQENSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSLLTTTEAVV EQ KD+KEAAPSMGG MGY
Sbjct: 541 SVSSLLTTTEAVVVEQPKDEKEAAPSMGG-MGY 572

BLAST of CmoCh08G000540 vs. ExPASy TrEMBL
Match: A0A0A0K893 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G332900 PE=3 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.8e-287
Identity = 536/573 (93.54%), Postives = 554/573 (96.68%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFA GLASKARLA NSA QINSR N SRNYAAK IKFGVEAR LMLRGVEELADAVKV
Sbjct: 1   MHRFASGLASKARLANNSARQINSRFNSSRNYAAKHIKFGVEARTLMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQS+GAPKVTKDGVTVAKSIEF+DKVKN+GASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSFGAPKVTKDGVTVAKSIEFEDKVKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVA+GVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVASGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKA+EKVGKEGVITIS+GNTLDNELEIVEGMKLDRG+ISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKALEKVGKEGVITISDGNTLDNELEIVEGMKLDRGYISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVES+ALATLILNKLRA
Sbjct: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESEALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKA MQDLAILTG QVITEEL L+LEKVG ESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKAGMQDLAILTGAQVITEELSLNLEKVGFESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TV+LDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVILDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GE+KDRVTDA+NAT+AAVEEGIVAGGGVALLYASKELEKL TANFDQKIGVQIIQNALKM
Sbjct: 421 GEKKDRVTDAVNATRAAVEEGIVAGGGVALLYASKELEKLQTANFDQKIGVQIIQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGA+V GKLL+QDN +LGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAIVIGKLLQQDNHELGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSL+TTTEAVV EQ  D+KEA PSMGG MGY
Sbjct: 541 SVSSLMTTTEAVVVEQPNDEKEATPSMGG-MGY 572

BLAST of CmoCh08G000540 vs. ExPASy TrEMBL
Match: A0A6P3Z8S5 (chaperonin CPN60-2, mitochondrial OS=Ziziphus jujuba OX=326968 GN=LOC107408563 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 6.8e-275
Identity = 509/572 (88.99%), Postives = 546/572 (95.45%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           M+RFA  LASKAR+ARNS +Q  SRL+ SRNYAAKDIKFGVEARALMLRGVEELADAVKV
Sbjct: 1   MYRFASSLASKARIARNSTYQNGSRLSWSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQS+GAPKVTKDGVTVAKS+EFKD+VKN+GASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSFGAPKVTKDGVTVAKSVEFKDRVKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAG+NAMDLRRGITMAVD+V+T LKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGMNAMDLRRGITMAVDAVVTNLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+G TL NELE+VEGMKLDRG+ISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGKTLFNELEVVEGMKLDRGYISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDPLILIHEKKIS++NAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA
Sbjct: 241 NQKCELEDPLILIHEKKISSINAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFG+NRKA++QDLA+LTGG +ITEELGL+LEKV  + LG+CKKVT+SKDD
Sbjct: 301 GIKVCAIKAPGFGENRKANLQDLAVLTGGDLITEELGLNLEKVDLDMLGTCKKVTISKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDKKAI+E+ +Q+RS IELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGAGDKKAIDERCEQIRSAIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
            E+KDRVTDALNATKAAVEEGIV GGGVALLYASKELEKLHTANFDQKIGVQIIQNALK 
Sbjct: 421 SEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKT 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           P++TIASNAGVEGAVV GKLLEQD+ DLGYDAAKGEYVDM+KAGIIDPLKVIRTALVDAA
Sbjct: 481 PVHTIASNAGVEGAVVVGKLLEQDDPDLGYDAAKGEYVDMVKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMG 573
           SVSSL+TTTEA+V E  KDDKE  P+MGG MG
Sbjct: 541 SVSSLMTTTEAIVVELPKDDKE-VPAMGGGMG 571

BLAST of CmoCh08G000540 vs. NCBI nr
Match: XP_022959552.1 (chaperonin CPN60-2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 1063.5 bits (2749), Expect = 6.3e-307
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV
Sbjct: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA
Sbjct: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM
Sbjct: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY
Sbjct: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 573

BLAST of CmoCh08G000540 vs. NCBI nr
Match: XP_023513641.1 (chaperonin CPN60-2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.2e-304
Identity = 567/573 (98.95%), Postives = 570/573 (99.48%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFACGLASKARLARNS+HQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV
Sbjct: 1   MHRFACGLASKARLARNSSHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVIT LKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITILKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+GNTLDNELEIVEGMKLDRGFISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGNTLDNELEIVEGMKLDRGFISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA
Sbjct: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQI+QNALKM
Sbjct: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIVQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSLLTTTEAVV EQA DDKEAAPSMGGAMGY
Sbjct: 541 SVSSLLTTTEAVVVEQANDDKEAAPSMGGAMGY 573

BLAST of CmoCh08G000540 vs. NCBI nr
Match: KAG6592855.1 (Chaperonin CPN60-2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1053.9 bits (2724), Expect = 5.0e-304
Identity = 568/573 (99.13%), Postives = 569/573 (99.30%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFACGLASKA LARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV
Sbjct: 1   MHRFACGLASKAGLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+GNTLDNELEIVEGMKLDRGFISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGNTLDNELEIVEGMKLDRGFISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA
Sbjct: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKEL KLHTANFDQKIGVQIIQNALKM
Sbjct: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELVKLHTANFDQKIGVQIIQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDA KGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDATKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSLLTTTEAVV EQAKDDKEAAPSMGGAMGY
Sbjct: 541 SVSSLLTTTEAVVVEQAKDDKEAAPSMGGAMGY 573

BLAST of CmoCh08G000540 vs. NCBI nr
Match: XP_023004983.1 (chaperonin CPN60-2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.7e-302
Identity = 564/573 (98.43%), Postives = 567/573 (98.95%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFACGLASKARLARNSAH INSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV
Sbjct: 1   MHRFACGLASKARLARNSAHLINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+GNTLDNELEIVEGMK+DRGFISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGNTLDNELEIVEGMKMDRGFISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVES+ALATLILNKLRA
Sbjct: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESEALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKA MQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKAGMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDG GDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGTGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELE LHTANFDQKIGVQIIQNALKM
Sbjct: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELENLHTANFDQKIGVQIIQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSLLTTTEAVV EQAKD KEAAPSMGGAMGY
Sbjct: 541 SVSSLLTTTEAVVVEQAKDGKEAAPSMGGAMGY 573

BLAST of CmoCh08G000540 vs. NCBI nr
Match: XP_022150736.1 (chaperonin CPN60-2, mitochondrial-like [Momordica charantia])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.4e-293
Identity = 548/573 (95.64%), Postives = 563/573 (98.25%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           MHRFACGLASKARLA+N+AHQINSRLN SRNYAAKDIKFGVEARA MLRGVEELADAVKV
Sbjct: 1   MHRFACGLASKARLAKNTAHQINSRLNWSRNYAAKDIKFGVEARASMLRGVEELADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGA LVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGAGLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMA+DSV++TLKSRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAIDSVVSTLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITIS+GNTLDNELEIVEGMKLDRG+ISPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGNTLDNELEIVEGMKLDRGYISPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
           NQKCELEDP+ILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVES+ALATLILNKLRA
Sbjct: 241 NQKCELEDPIILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESEALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFGDNRKA++QDLAILTGGQVITEELGLDLEKVG ESLGSCKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGDNRKANLQDLAILTGGQVITEELGLDLEKVGFESLGSCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDKKAIEE+SDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVVLDGAGDKKAIEERSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GE+KDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKL T NFDQKIGVQIIQNALKM
Sbjct: 421 GEKKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLQTPNFDQKIGVQIIQNALKM 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           PIYTIASNAGVEGAVV GKLLEQ+NSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PIYTIASNAGVEGAVVIGKLLEQENSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGGAMGY 574
           SVSSLLTTTEAVV EQ KD+KEAAPSMGG MGY
Sbjct: 541 SVSSLLTTTEAVVVEQPKDEKEAAPSMGG-MGY 572

BLAST of CmoCh08G000540 vs. TAIR 10
Match: AT3G23990.1 (heat shock protein 60 )

HSP 1 Score: 919.1 bits (2374), Expect = 1.8e-267
Identity = 489/575 (85.04%), Postives = 538/575 (93.57%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           M+RFA  LASKAR+A+N A Q++SR++ SRNYAAK+IKFGVEARALML+GVE+LADAVKV
Sbjct: 1   MYRFASNLASKARIAQN-ARQVSSRMSWSRNYAAKEIKFGVEARALMLKGVEDLADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNVV+EQS+GAPKVTKDGVTVAKSIEFKDK+KN+GASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVVIEQSWGAPKVTKDGVTVAKSIEFKDKIKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIF EGCKSVAAG+NAMDLRRGI+MAVD+V+T LKS+ARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFAEGCKSVAAGMNAMDLRRGISMAVDAVVTNLKSKARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIAKAMEKVGKEGVITI +G TL NELE+VEGMKLDRG+ SPYFITNQK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITIQDGKTLFNELEVVEGMKLDRGYTSPYFITNQK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
            QKCEL+DPLILIHEKKIS++N++VKVLELALKRQRPLLIV+EDVESDALATLILNKLRA
Sbjct: 241 TQKCELDDPLILIHEKKISSINSIVKVLELALKRQRPLLIVSEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           GIKVCAIKAPGFG+NRKA++QDLA LTGG+VIT+ELG++LEKV    LG+CKKVTVSKDD
Sbjct: 301 GIKVCAIKAPGFGENRKANLQDLAALTGGEVITDELGMNLEKVDLSMLGTCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TV+LDGAGDKK IEE+ +Q+RS IELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV
Sbjct: 361 TVILDGAGDKKGIEERCEQIRSAIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GE+KDRVTDALNATKAAVEEGI+ GGGVALLYA++ELEKL TANFDQKIGVQIIQNALK 
Sbjct: 421 GEKKDRVTDALNATKAAVEEGILPGGGVALLYAARELEKLPTANFDQKIGVQIIQNALKT 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           P+YTIASNAGVEGAV+ GKLLEQDN DLGYDAAKGEYVDM+KAGIIDPLKVIRTALVDAA
Sbjct: 481 PVYTIASNAGVEGAVIVGKLLEQDNPDLGYDAAKGEYVDMVKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKE---AAPSMGGAMG 573
           SVSSLLTTTEAVV +  KD+ E   A   MGG  G
Sbjct: 541 SVSSLLTTTEAVVVDLPKDESESGAAGAGMGGMGG 574

BLAST of CmoCh08G000540 vs. TAIR 10
Match: AT2G33210.1 (heat shock protein 60-2 )

HSP 1 Score: 901.0 bits (2327), Expect = 5.0e-262
Identity = 479/569 (84.18%), Postives = 523/569 (91.92%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           M+R    +ASKAR+AR    QI SRLN +RNYAAKDI+FGVEARALMLRGVE+LADAVKV
Sbjct: 1   MYRLVSNVASKARIARKCTSQIGSRLNSTRNYAAKDIRFGVEARALMLRGVEDLADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNV++EQS+GAPKVTKDGVTVAKSIEFKD++KN+GASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVIIEQSWGAPKVTKDGVTVAKSIEFKDRIKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAG+NAMDLRRGI +AVD+V+T L+SRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGMNAMDLRRGIKLAVDTVVTNLQSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANG+REIGELIAKAME VGKEGVITI +G TL NELE+VEGMK+DRG+ISPYFITN K
Sbjct: 181 ISANGDREIGELIAKAMETVGKEGVITIQDGKTLFNELEVVEGMKIDRGYISPYFITNPK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
            QKCELEDPLILIHEKKISN+NA+VKVLELALK+QRPLLIVAEDVESDALATLILNKLRA
Sbjct: 241 TQKCELEDPLILIHEKKISNINAMVKVLELALKKQRPLLIVAEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
            IKVCA+KAPGFG+NRKA++ DLA LTG QVITEELG++L+ +     G+CKKVTVSKDD
Sbjct: 301 NIKVCAVKAPGFGENRKANLHDLAALTGAQVITEELGMNLDNIDLSMFGNCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDK+AI E+ +Q+RS++E STSDYDKEKLQERLAKLSGGVAVLKIGGASE EV
Sbjct: 361 TVVLDGAGDKQAIGERCEQIRSMVEASTSDYDKEKLQERLAKLSGGVAVLKIGGASETEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
            E+KDRVTDALNATKAAVEEGIV GGGVALLYASKELEKL TANFDQKIGVQIIQNALK 
Sbjct: 421 SEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELEKLSTANFDQKIGVQIIQNALKT 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           P+YTIASNAGVEGAVV GKLLEQDN DLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PVYTIASNAGVEGAVVVGKLLEQDNPDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGG 570
           SVSSLLTTTEAVV E     + A+P MGG
Sbjct: 541 SVSSLLTTTEAVVTE-IPTKEVASPGMGG 568

BLAST of CmoCh08G000540 vs. TAIR 10
Match: AT2G33210.2 (heat shock protein 60-2 )

HSP 1 Score: 885.2 bits (2286), Expect = 2.9e-257
Identity = 475/569 (83.48%), Postives = 518/569 (91.04%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           M+R    +ASKAR+AR    QI SRLN +RNYAAKDI+FGVEARALMLRGVE+LADAVKV
Sbjct: 1   MYRLVSNVASKARIARKCTSQIGSRLNSTRNYAAKDIRFGVEARALMLRGVEDLADAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNV++EQS+GAPKVTKDGVTVAKSIEFKD++KN+GASLVKQVANATNDVAGDGT
Sbjct: 61  TMGPKGRNVIIEQSWGAPKVTKDGVTVAKSIEFKDRIKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLTRAIFTEGCKSVAAG+NAMDLRRGI +AVD+V+T L+SRARMISTSEEIAQVGT
Sbjct: 121 TCATVLTRAIFTEGCKSVAAGMNAMDLRRGIKLAVDTVVTNLQSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANG+REIGELIAKAME VGKEGVITI +G TL NELE+VEGMK+DRG+ISPYFITN K
Sbjct: 181 ISANGDREIGELIAKAMETVGKEGVITIQDGKTLFNELEVVEGMKIDRGYISPYFITNPK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
            QKCELEDPLILIHEKKISN+NA+VKVLELALK+QRPLLIVAEDVESDALATLILNKLRA
Sbjct: 241 TQKCELEDPLILIHEKKISNINAMVKVLELALKKQRPLLIVAEDVESDALATLILNKLRA 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
                 IKAPGFG+NRKA++ DLA LTG QVITEELG++L+ +     G+CKKVTVSKDD
Sbjct: 301 N-----IKAPGFGENRKANLHDLAALTGAQVITEELGMNLDNIDLSMFGNCKKVTVSKDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           TVVLDGAGDK+AI E+ +Q+RS++E STSDYDKEKLQERLAKLSGGVAVLKIGGASE EV
Sbjct: 361 TVVLDGAGDKQAIGERCEQIRSMVEASTSDYDKEKLQERLAKLSGGVAVLKIGGASETEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
            E+KDRVTDALNATKAAVEEGIV GGGVALLYASKELEKL TANFDQKIGVQIIQNALK 
Sbjct: 421 SEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELEKLSTANFDQKIGVQIIQNALKT 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           P+YTIASNAGVEGAVV GKLLEQDN DLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA
Sbjct: 481 PVYTIASNAGVEGAVVVGKLLEQDNPDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDDKEAAPSMGG 570
           SVSSLLTTTEAVV E     + A+P MGG
Sbjct: 541 SVSSLLTTTEAVVTE-IPTKEVASPGMGG 563

BLAST of CmoCh08G000540 vs. TAIR 10
Match: AT3G13860.1 (heat shock protein 60-3A )

HSP 1 Score: 756.1 bits (1951), Expect = 2.0e-218
Identity = 401/560 (71.61%), Postives = 476/560 (85.00%), Query Frame = 0

Query: 1   MHRFACGLASKARLARNSAHQINSRLNCSRNYAAKDIKFGVEARALMLRGVEELADAVKV 60
           M+R    L+S    +  S   ++ R+  SRNYAAKDI FG+ ARA ML+GV E+A+AVKV
Sbjct: 1   MYRVLSKLSSSIG-SSTSRKLVSGRIISSRNYAAKDISFGIGARAAMLQGVSEVAEAVKV 60

Query: 61  TMGPKGRNVVLEQSYGAPKVTKDGVTVAKSIEFKDKVKNIGASLVKQVANATNDVAGDGT 120
           TMGPKGRNV++E SYG PK+TKDGVTVAKSI F+ K KNIGA LVKQVA+ATN VAGDGT
Sbjct: 61  TMGPKGRNVIIESSYGGPKITKDGVTVAKSISFQAKAKNIGAELVKQVASATNKVAGDGT 120

Query: 121 TCATVLTRAIFTEGCKSVAAGVNAMDLRRGITMAVDSVITTLKSRARMISTSEEIAQVGT 180
           TCATVLT+AI  EGCKSVAAGVN MDLR GI MA+ +V++ LKSRA MIST EEI QV T
Sbjct: 121 TCATVLTQAILIEGCKSVAAGVNVMDLRVGINMAIAAVVSDLKSRAVMISTPEEITQVAT 180

Query: 181 ISANGEREIGELIAKAMEKVGKEGVITISEGNTLDNELEIVEGMKLDRGFISPYFITNQK 240
           ISANGEREIGELIA+AMEKVGKEGVIT+++GNTLDNELE+VEGMKL RG+ISPYFIT++K
Sbjct: 181 ISANGEREIGELIARAMEKVGKEGVITVADGNTLDNELEVVEGMKLARGYISPYFITDEK 240

Query: 241 NQKCELEDPLILIHEKKISNLNAVVKVLELALKRQRPLLIVAEDVESDALATLILNKLRA 300
            QKCELE+P+ILIHEKKIS++N+++KVLE A+K  RPLLIVAEDVESDALA LILNK   
Sbjct: 241 TQKCELENPIILIHEKKISDINSLLKVLEAAVKSSRPLLIVAEDVESDALAMLILNKHHG 300

Query: 301 GIKVCAIKAPGFGDNRKASMQDLAILTGGQVITEELGLDLEKVGAESLGSCKKVTVSKDD 360
           G+KVCAIKAPGFGDNRKAS+ DLA+LTG +VI+EE GL LEK+  E LG+ KKVTV++DD
Sbjct: 301 GLKVCAIKAPGFGDNRKASLDDLAVLTGAEVISEERGLSLEKIRPELLGTAKKVTVTRDD 360

Query: 361 TVVLDGAGDKKAIEEQSDQLRSLIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420
           T++L G GDKK IEE+ ++LRS  E STS +D+EK QERL+KLSGGVAV K+GGASE+EV
Sbjct: 361 TIILHGGGDKKLIEERCEELRSANEKSTSTFDQEKTQERLSKLSGGVAVFKVGGASESEV 420

Query: 421 GERKDRVTDALNATKAAVEEGIVAGGGVALLYASKELEKLHTANFDQKIGVQIIQNALKM 480
           GERKDRVTDALNAT+AAVEEGI+ GGGVALLYA+K L+ L T N DQ+ GVQI+QNALK 
Sbjct: 421 GERKDRVTDALNATRAAVEEGIIPGGGVALLYATKALDNLQTENEDQRRGVQIVQNALKA 480

Query: 481 PIYTIASNAGVEGAVVAGKLLEQDNSDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540
           P +TIA+NAG +G++V GKLLEQD+ + G+DAAKG+YVDM+KAGIIDP+KVIRTAL DAA
Sbjct: 481 PAFTIAANAGYDGSLVVGKLLEQDDCNFGFDAAKGKYVDMVKAGIIDPVKVIRTALTDAA 540

Query: 541 SVSSLLTTTEAVVAEQAKDD 561
           SVS LLTTTEA V  +A ++
Sbjct: 541 SVSLLLTTTEASVLVKADEN 559

BLAST of CmoCh08G000540 vs. TAIR 10
Match: AT5G56500.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 485.0 bits (1247), Expect = 8.6e-137
Identity = 260/539 (48.24%), Postives = 373/539 (69.20%), Query Frame = 0

Query: 32  YAAKDIKFGVEARAL--MLRGVEELADAVKVTMGPKGRNVVLEQSYGAPKVTKDGVTVAK 91
           YAAK + F  +  A+  +  GV +LAD V VT+GPKGRNVVLE  YG+P++  DGVTVA+
Sbjct: 50  YAAKQLHFNKDGTAIKKLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAR 109

Query: 92  SIEFKDKVKNIGASLVKQVANATNDVAGDGTTCATVLTRAIFTEGCKSVAAGVNAMDLRR 151
            +E +D V+NIGA LV+Q A+ TND+AGDGTT + VL + +  EG K VAAG N + + R
Sbjct: 110 EVELEDPVENIGAKLVRQAASKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITR 169

Query: 152 GITMAVDSVITTLKSRARMISTSEEIAQVGTISANGEREIGELIAKAMEKVGKEGVITIS 211
           GI     +++  LK  ++ +  S E+A V  +SA    E+G +IA+AM KVG++GV+T+ 
Sbjct: 170 GIEKTTKALVAELKKMSKEVEDS-ELADVAAVSAGNNYEVGNMIAEAMAKVGRKGVVTLE 229

Query: 212 EGNTLDNELEIVEGMKLDRGFISPYFITNQKNQKCELEDPLILIHEKKISNLNAVVKVLE 271
           EG + +N L +VEGM+ DRG+ISPYF+T+ +    E E+  + + +KKI+N   ++ +LE
Sbjct: 230 EGKSAENSLYVVEGMQFDRGYISPYFVTDSEKMCAEYENCKLFLVDKKITNARDIISILE 289

Query: 272 LALKRQRPLLIVAEDVESDALATLILNKLRAGIKVCAIKAPGFGDNRKASMQDLAILTGG 331
            A+K   PLLI+AED+E + LATL++NKLR  IKV A+KAPGFG+ +   + D+A LTG 
Sbjct: 290 DAIKGGYPLLIIAEDIEQEPLATLVVNKLRGTIKVAALKAPGFGERKSQYLDDIAALTGA 349

Query: 332 QVITEELGLDLEKVGAESLGSCKKVTVSKDDTVVLDGAGDKKAIEEQSDQLRSLIELSTS 391
            VI EE+GL LEKVG E LG+  KV ++KD T ++     ++ ++++ +Q+++LIE +  
Sbjct: 350 TVIREEVGLQLEKVGPEVLGNAGKVVLTKDTTTIVGDGSTEEVVKKRVEQIKNLIEAAEQ 409

Query: 392 DYDKEKLQERLAKLSGGVAVLKIGGASEAEVGERKDRVTDALNATKAAVEEGIVAGGGVA 451
           DY+KEKL ER+AKLSGGVAV+++G  +E E+ E+K RV DALNATKAAVEEGIV GGG  
Sbjct: 410 DYEKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCT 469

Query: 452 LLYASKELE--KLHTANFDQKIGVQIIQNALKMPIYTIASNAGVEGAVVAGKLLEQDNSD 511
           LL  + +++  K   AN ++K+G  I++ AL  P+  IA NAGV G+VV+ K+L  DN  
Sbjct: 470 LLRLASKVDAIKETLANDEEKVGADIVKKALSYPLKLIAKNAGVNGSVVSEKVLSSDNPK 529

Query: 512 LGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAASVSSLLTTTEAVVAEQAKDDKEAAPS 567
            GY+AA G+Y D++ AGIIDP KV+R  L  A+SV+     ++ VV E  K+ + AAP+
Sbjct: 530 HGYNAATGKYEDLMAAGIIDPTKVVRCCLEHASSVAKTFLMSDCVVVE-IKEPESAAPA 586

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q050461.3e-27587.94Chaperonin CPN60-2, mitochondrial OS=Cucurbita maxima OX=3661 GN=CPN60-2 PE=1 SV... [more]
Q050456.2e-27386.89Chaperonin CPN60-1, mitochondrial OS=Cucurbita maxima OX=3661 GN=CPN60-1 PE=1 SV... [more]
P291972.5e-26685.04Chaperonin CPN60, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CPN60 PE=1 SV... [more]
P291859.9e-26383.80Chaperonin CPN60-1, mitochondrial OS=Zea mays OX=4577 GN=CPN60I PE=1 SV=2[more]
Q432982.9e-26284.15Chaperonin CPN60-2, mitochondrial OS=Zea mays OX=4577 GN=CPN60II PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H4V23.0e-307100.00chaperonin CPN60-2, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1L0Z81.3e-30298.43chaperonin CPN60-2, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC1114981... [more]
A0A6J1DCE46.6e-29495.64chaperonin CPN60-2, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A0A0K8937.8e-28793.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G332900 PE=3 SV=1[more]
A0A6P3Z8S56.8e-27588.99chaperonin CPN60-2, mitochondrial OS=Ziziphus jujuba OX=326968 GN=LOC107408563 P... [more]
Match NameE-valueIdentityDescription
XP_022959552.16.3e-307100.00chaperonin CPN60-2, mitochondrial-like [Cucurbita moschata][more]
XP_023513641.12.2e-30498.95chaperonin CPN60-2, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
KAG6592855.15.0e-30499.13Chaperonin CPN60-2, mitochondrial, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023004983.12.7e-30298.43chaperonin CPN60-2, mitochondrial-like [Cucurbita maxima][more]
XP_022150736.11.4e-29395.64chaperonin CPN60-2, mitochondrial-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G23990.11.8e-26785.04heat shock protein 60 [more]
AT2G33210.15.0e-26284.18heat shock protein 60-2 [more]
AT2G33210.22.9e-25783.48heat shock protein 60-2 [more]
AT3G13860.12.0e-21871.61heat shock protein 60-3A [more]
AT5G56500.18.6e-13748.24TCP-1/cpn60 chaperonin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001844Chaperonin Cpn60PRINTSPR00298CHAPERONIN60coord: 299..322
score: 60.18
coord: 429..450
score: 81.69
coord: 381..406
score: 70.37
coord: 58..84
score: 70.34
coord: 114..141
score: 65.62
IPR001844Chaperonin Cpn60TIGRFAMTIGR02348TIGR02348coord: 34..558
e-value: 1.9E-224
score: 743.9
IPR001844Chaperonin Cpn60HAMAPMF_00600CH60coord: 33..573
score: 37.298603
IPR001844Chaperonin Cpn60CDDcd03344GroELcoord: 35..555
e-value: 0.0
score: 865.23
IPR002423Chaperonin Cpn60/TCP-1 familyPFAMPF00118Cpn60_TCP1coord: 54..555
e-value: 5.1E-84
score: 282.7
IPR027413GroEL-like equatorial domain superfamilyGENE3D1.10.560.10coord: 37..554
e-value: 3.1E-233
score: 776.9
IPR027413GroEL-like equatorial domain superfamilySUPERFAMILY48592GroEL equatorial domain-likecoord: 37..553
IPR027409GroEL-like apical domain superfamilyGENE3D3.50.7.10GroELcoord: 223..404
e-value: 3.1E-233
score: 776.9
IPR027409GroEL-like apical domain superfamilySUPERFAMILY52029GroEL apical domain-likecoord: 215..407
IPR027410TCP-1-like chaperonin intermediate domain superfamilyGENE3D3.30.260.10coord: 168..441
e-value: 3.1E-233
score: 776.9
IPR027410TCP-1-like chaperonin intermediate domain superfamilySUPERFAMILY54849GroEL-intermediate domain likecoord: 169..236
NoneNo IPR availablePANTHERPTHR45633:SF40CHAPERONIN CPN60-2, MITOCHONDRIAL-LIKEcoord: 13..568
NoneNo IPR availablePANTHERPTHR4563360 KDA HEAT SHOCK PROTEIN, MITOCHONDRIALcoord: 13..568
IPR018370Chaperonin Cpn60, conserved sitePROSITEPS00296CHAPERONINS_CPN60coord: 436..447

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G000540.1CmoCh08G000540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042026 protein refolding
biological_process GO:0006457 protein folding
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity