CmoCh08G000350 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G000350
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Cytochrome P450
Location	Cmo_Chr08: 183408 .. 185542 (-)
RNA-Seq Expression	CmoCh08G000350
Synteny	CmoCh08G000350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCTCCAATTATCAATGTGGTCGCCGGGATTAGCCATCGGAGCAGTGGTGATAATACTTTTGACCCACTTGGTTTACAGGTGGAGAAATCCTAAATGTAATGGAGTCCTTCCTCCGGGCTCCATGGGCTTCCCTCTCATCGGAGAGACCATTCAGCTTATAGCTTCCGGCAACACTCTGGACCTTCCTCCCTTTATCAAGAAGAGAGTGCAGAAGTAAGGCGGTGGGCGGAGGAGATTTGTATTGGGTTATACTGTTATTATAATAAGATGTTTGATTAATCTCGAGTTTGAATTATTACAGATATGGACCCATATTTCGGACCAGTCTGGTGGGTCGATCCATTGTGGTTACGGCTGACCCTGAAATCAACAGTTTCATTTACGCTCAAGAAGGGAAGACGGTGGAGCTCTGGTATTTGGACTCCATTTCCAAGGTGCTCAAGCAAGACGGCGACACTAGGACAACCGCGGGCGGCGTGATTCATAAGTACCTCAGAGGTATCACTTTGAACCACTTTGGCTCCGAAAGTCTCAAGACGAAGCTGCTTGCTGATATCCAGCGATGCGTCGACAAGGTTCTGGTCCAGTGGTCCAGCCGTCCGTCTGTTGAAATGCAGCACGGGACTCTCGCTGTAAGTTTCGCATCTTAATTGCTAAAATGGTGTCTTCCACATCACGTACTGACCCCGACAACCAACCAGATGCTATACGATTTCAACGCGGATGTGATGTTCGGATATGACCCAGAGAAGTCGAGCGAGAGTATCAGCGAGAGCTTAATCACATTAGCAGACGGGTTTATGGCGGTCCCGTTGAACTTGCCAGGAACGAAGTACAACAAATGTCTCAAGGCGCAAAAGAAGCTGGTCAACATGTTCAAGGATCTTGTCAAGGAGAGGCGTCAAGCTGCTGCTGCTAATGCTCGAGGGGATTTTCTTGATCAGGCCCTGCGCGACATTGGCAGCGAACAATTTCTATCTGAGGAATTCGTTGTGAATTTACTGTTCGGAATTTTATTCGCTAGTGGGTCGGTGTCCGGATCGCTCACCTTGATGCTCAAGCTACTTGCAGAGAATCCATCTGTGGTGCGGGAGTTGACTGTAAGTAATTAATCCATCGGTGGAGTGTGTTAGTTGCTACCAACGTAGGCTCTGTGTCTCTATGTTTAATTTTTGTTTGTGGGTGTAATTTTTGTTTGTGGGTGTGTGAGTGTGATCGATATTTCTTTCGCTTCCATTACAACACTCTGCCTGCATAATTGTGGATTGTTTGACTCTTATTTAACACAATTTAACTGATCGGCAGGCCGAGCATGAAGCGTTTCTTAAACAGAGGAAGGATCCAGAATCTCCCATCACTTGGGAGGAGTATAAGTCAATGACATTTACGCTTCAAGTAGGAAGTTACGGTTCGAAAATTAAGTTTATAAACACTACTTTATTTTGGTTCCTAAAGATGTAGGGTTGTGCATATAGGTTATATATGAAACTCTGAGGTTGTCAAACGCACTGCCATTTCTACTGCGGCGAACAATAAAGGACCTGAACATCAAAGGTAAGAAGCTGGTCATTTACAATTGTGTTGATAATGAGAGTCTTTTTAGACGAGTGATGAATTGAAATGGTGGTGGAAATGCTGTGTAGGATATACAATTCCAGCGGGGTGGACGCTTATGGTTGCAAACTCAGCTCTCCACTTGAACCCTGGCACCCACAAGGATCCATTGGACTTCAACCCATGGCGGTGGAAGGTATATTATGGACTATTATAGGACAGGTTATAGGTAGGAAGGGATGATATGCAGGTGATTGGTTTGTAATATTGTTGCAGGACCTAGACCAACACTCAATTTCGAAGAACTTGCAGCCGTTTGGAGGAGGATCTCGGCAATGCGCTGGGGCAGATTACAGCAGGGTTTTCATGGCCATCTTCCTCCACACTCTGCTCACTAAGTACAGGTATATTTTACGAACCACTAGATACAAATTCGAAATACTTTCGTGTTCTATTCTGAGACGAGTCGGGTTTGTTGGGTGCAGTTGGAGAAAGGTGAAGGGAGGAGACATTTCTCGAAGTCCTATTCTGAAATTTGGAGATGGGCTTCATATCAGCTTCAAAGCCAAGGCTTAG

mRNA sequence

ATGGAGCTCCAATTATCAATGTGGTCGCCGGGATTAGCCATCGGAGCAGTGGTGATAATACTTTTGACCCACTTGGTTTACAGGTGGAGAAATCCTAAATGTAATGGAGTCCTTCCTCCGGGCTCCATGGGCTTCCCTCTCATCGGAGAGACCATTCAGCTTATAGCTTCCGGCAACACTCTGGACCTTCCTCCCTTTATCAAGAAGAGAGTGCAGAAATATGGACCCATATTTCGGACCAGTCTGGTGGGTCGATCCATTGTGGTTACGGCTGACCCTGAAATCAACAGTTTCATTTACGCTCAAGAAGGGAAGACGGTGGAGCTCTGGTATTTGGACTCCATTTCCAAGGTGCTCAAGCAAGACGGCGACACTAGGACAACCGCGGGCGGCGTGATTCATAAGTACCTCAGAGGTATCACTTTGAACCACTTTGGCTCCGAAAGTCTCAAGACGAAGCTGCTTGCTGATATCCAGCGATGCGTCGACAAGGTTCTGGTCCAGTGGTCCAGCCGTCCGTCTGTTGAAATGCAGCACGGGACTCTCGCTATGCTATACGATTTCAACGCGGATGTGATGTTCGGATATGACCCAGAGAAGTCGAGCGAGAGTATCAGCGAGAGCTTAATCACATTAGCAGACGGGTTTATGGCGGTCCCGTTGAACTTGCCAGGAACGAAGTACAACAAATGTCTCAAGGCGCAAAAGAAGCTGGTCAACATGTTCAAGGATCTTGTCAAGGAGAGGCGTCAAGCTGCTGCTGCTAATGCTCGAGGGGATTTTCTTGATCAGGCCCTGCGCGACATTGGCAGCGAACAATTTCTATCTGAGGAATTCGTTGTGAATTTACTGTTCGGAATTTTATTCGCTAGTGGGTCGGTGTCCGGATCGCTCACCTTGATGCTCAAGCTACTTGCAGAGAATCCATCTGTGGTGCGGGAGTTGACTGCCGAGCATGAAGCGTTTCTTAAACAGAGGAAGGATCCAGAATCTCCCATCACTTGGGAGGAGTATAAGTCAATGACATTTACGCTTCAAGTTATATATGAAACTCTGAGGTTGTCAAACGCACTGCCATTTCTACTGCGGCGAACAATAAAGGACCTGAACATCAAAGGATATACAATTCCAGCGGGGTGGACGCTTATGGTTGCAAACTCAGCTCTCCACTTGAACCCTGGCACCCACAAGGATCCATTGGACTTCAACCCATGGCGGTGGAAGGACCTAGACCAACACTCAATTTCGAAGAACTTGCAGCCGTTTGGAGGAGGATCTCGGCAATGCGCTGGGGCAGATTACAGCAGGGTTTTCATGGCCATCTTCCTCCACACTCTGCTCACTAAGTACAGGTATATTTTACGAACCACTAGATACAAATTCGAAATACTTTCGTGTTCTATTCTGAGACGAGTCGGGTTTGTTGGGTGCAGTTGGAGAAAGGTGAAGGGAGGAGACATTTCTCGAAGTCCTATTCTGAAATTTGGAGATGGGCTTCATATCAGCTTCAAAGCCAAGGCTTAG

Coding sequence (CDS)

ATGGAGCTCCAATTATCAATGTGGTCGCCGGGATTAGCCATCGGAGCAGTGGTGATAATACTTTTGACCCACTTGGTTTACAGGTGGAGAAATCCTAAATGTAATGGAGTCCTTCCTCCGGGCTCCATGGGCTTCCCTCTCATCGGAGAGACCATTCAGCTTATAGCTTCCGGCAACACTCTGGACCTTCCTCCCTTTATCAAGAAGAGAGTGCAGAAATATGGACCCATATTTCGGACCAGTCTGGTGGGTCGATCCATTGTGGTTACGGCTGACCCTGAAATCAACAGTTTCATTTACGCTCAAGAAGGGAAGACGGTGGAGCTCTGGTATTTGGACTCCATTTCCAAGGTGCTCAAGCAAGACGGCGACACTAGGACAACCGCGGGCGGCGTGATTCATAAGTACCTCAGAGGTATCACTTTGAACCACTTTGGCTCCGAAAGTCTCAAGACGAAGCTGCTTGCTGATATCCAGCGATGCGTCGACAAGGTTCTGGTCCAGTGGTCCAGCCGTCCGTCTGTTGAAATGCAGCACGGGACTCTCGCTATGCTATACGATTTCAACGCGGATGTGATGTTCGGATATGACCCAGAGAAGTCGAGCGAGAGTATCAGCGAGAGCTTAATCACATTAGCAGACGGGTTTATGGCGGTCCCGTTGAACTTGCCAGGAACGAAGTACAACAAATGTCTCAAGGCGCAAAAGAAGCTGGTCAACATGTTCAAGGATCTTGTCAAGGAGAGGCGTCAAGCTGCTGCTGCTAATGCTCGAGGGGATTTTCTTGATCAGGCCCTGCGCGACATTGGCAGCGAACAATTTCTATCTGAGGAATTCGTTGTGAATTTACTGTTCGGAATTTTATTCGCTAGTGGGTCGGTGTCCGGATCGCTCACCTTGATGCTCAAGCTACTTGCAGAGAATCCATCTGTGGTGCGGGAGTTGACTGCCGAGCATGAAGCGTTTCTTAAACAGAGGAAGGATCCAGAATCTCCCATCACTTGGGAGGAGTATAAGTCAATGACATTTACGCTTCAAGTTATATATGAAACTCTGAGGTTGTCAAACGCACTGCCATTTCTACTGCGGCGAACAATAAAGGACCTGAACATCAAAGGATATACAATTCCAGCGGGGTGGACGCTTATGGTTGCAAACTCAGCTCTCCACTTGAACCCTGGCACCCACAAGGATCCATTGGACTTCAACCCATGGCGGTGGAAGGACCTAGACCAACACTCAATTTCGAAGAACTTGCAGCCGTTTGGAGGAGGATCTCGGCAATGCGCTGGGGCAGATTACAGCAGGGTTTTCATGGCCATCTTCCTCCACACTCTGCTCACTAAGTACAGGTATATTTTACGAACCACTAGATACAAATTCGAAATACTTTCGTGTTCTATTCTGAGACGAGTCGGGTTTGTTGGGTGCAGTTGGAGAAAGGTGAAGGGAGGAGACATTTCTCGAAGTCCTATTCTGAAATTTGGAGATGGGCTTCATATCAGCTTCAAAGCCAAGGCTTAG

Protein sequence

MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWRKVKGGDISRSPILKFGDGLHISFKAKA

Homology

[Close]

BLAST of CmoCh08G000350 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 7.2e-140
Identity = 248/501 (49.50%), Postives = 334/501 (66.67%), Query Frame = 0


Query: 7   MWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPF 66
           MW+  L +  + +    H + +WR+ K NGVLPPG+MG PLIGETIQL    ++LD+ PF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126
           I+K+V++YGPIF+T L GR +VV+AD E N++I  QEG+ VE+WYLD++SK    D +  
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEW- 120

Query: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186
             A G+IHKY+R ITLNHFG+E+L+ + L  I+    + L  WS++PSVE+++ +  M++
Sbjct: 121 LKALGLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVF 180

Query: 187 DFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLV 246
             + + MFG D +K S +I      L  GF+++PLN PGT Y+KCLK  K++    +++V
Sbjct: 181 RTSVNKMFGEDAKKLSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVV 240

Query: 247 KERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFAS-GSVSGSLTLMLKLL 306
            +R      +   DFL QAL+D  SE+F+SEEF++ LLF I FAS  S+S +LTL+LKLL
Sbjct: 241 DDRLANVGPDVE-DFLGQALKDKESEKFISEEFIIQLLFSISFASFESISTTLTLILKLL 300

Query: 307 AENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRT 366
            E+P VV+EL AEHEA  K R DP+ PITWEEYKSMTFTLQVI ETLRL +  P LLR+T
Sbjct: 301 DEHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLQVINETLRLGSVTPALLRKT 360

Query: 367 IKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQPFGGG 426
           +KDL +KGY IP GWT+M+  ++ H +P  +KDP  FNPWRWKDLD  +I KN  PFGGG
Sbjct: 361 VKDLQVKGYIIPEGWTIMLVTASRHRDPKVYKDPHIFNPWRWKDLDSITIQKNFMPFGGG 420

Query: 427 SRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWRKVKGG 486
            R CAGA+YS+V++  FLH L TKYR                           W K+ GG
Sbjct: 421 LRHCAGAEYSKVYLCTFLHILCTKYR---------------------------WTKLGGG 472

Query: 487 DISRSPILKFGDGLHISFKAK 507
            I+R+ IL F DGLH+ F  K
Sbjct: 481 TIARAHILSFEDGLHVKFTPK 472

[Close]

BLAST of CmoCh08G000350 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 418.3 bits (1074), Expect = 1.2e-115
Identity = 210/499 (42.08%), Postives = 308/499 (61.72%), Query Frame = 0


Query: 12  LAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPFIKKRV 71
           L      ++ L    YRW +P+ NG LPPGS+G P+IGET+Q  A   T DL PF+K+R+
Sbjct: 45  LCAALAAVVALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKERI 104

Query: 72  QKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTRTTAGG 131
           ++YG IF+TS+VGR +VV+ADPE+N +++ QEGK  E WY D+ +++  +  D   +  G
Sbjct: 105 KRYGSIFKTSVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGR--DNVGSLHG 164

Query: 132 VIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLYDFNAD 191
            ++KYL+ + L  +G E+LK+ LLA+        L  W+S+PSVE++ G   M++D  A 
Sbjct: 165 FMYKYLKTLVLRLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAK 224

Query: 192 VMFGYDPEKSSE-SISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLVKERR 251
            + GYDP K S+ ++ ++      G ++ PLN+PGT Y++C++ +K  + + + ++KE R
Sbjct: 225 KLIGYDPSKPSQVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGMMKE-R 284

Query: 252 QAAAANARGDFLDQALRDIGSEQ-FLSEEFVVNLLFGILFASGSVSG-SLTLMLKLLAEN 311
            A       DF D  ++++  E+  L+E   ++L+F +LFAS   +  +LT+ +KLL EN
Sbjct: 285 MAEPERPCEDFFDHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVKLLTEN 344

Query: 312 PSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRTIKD 371
           P VV  L  EHEA ++ RKDP S +TW EYKSMTFT QVI E +RL+N +P + R+ ++D
Sbjct: 345 PKVVDALREEHEAIIRNRKDPNSGVTWAEYKSMTFTSQVIMEIVRLANIVPGIFRKALQD 404

Query: 372 LNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQ-HSISKNLQPFGGGSR 431
           + IKGYTIPAGW +MV   A+HLNP  ++DPL FNPWRW+   +    +K+   FGGG R
Sbjct: 405 VEIKGYTIPAGWGIMVCPPAVHLNPEIYEDPLAFNPWRWQGKPEITGGTKHFMAFGGGLR 464

Query: 432 QCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWRKVKGGDI 491
            C G D S+V MA F+H+L+TKY                           SWR VKGG+I
Sbjct: 465 FCVGTDLSKVLMATFIHSLVTKY---------------------------SWRTVKGGNI 513

Query: 492 SRSPILKFGDGLHISFKAK 507
            R+P L F DG HI    K
Sbjct: 525 VRTPGLSFPDGFHIQLFPK 513

[Close]

BLAST of CmoCh08G000350 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 306.2 bits (783), Expect = 6.8e-82
Identity = 171/459 (37.25%), Postives = 261/459 (56.86%), Query Frame = 0


Query: 7   MWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPF 66
           +WS  + + AV  ++++  +YRW NPKCNG LPPGSMG P+IGET          ++ PF
Sbjct: 4   VWSAAVWVIAVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPF 63

Query: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126
           +KKR+ KYGP+FRT++ G + VV  +P+I   ++ QE K+    Y ++  K   +  +  
Sbjct: 64  VKKRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGK--ENV 123

Query: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186
               G IHK+++ I+L H GSE+LK K++ +I R   + L   +++ S + +    ++  
Sbjct: 124 FLKHGNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESV-- 183

Query: 187 DFNADVMFGYDPEKSSESISESLITLADGFMAV-------PLNLPG-TKYNKCLKAQKKL 246
                +M    P+  S    E+  TL D  MA+       PL L       K   A++  
Sbjct: 184 -----IMAHLTPKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYA 243

Query: 247 VNMFKDLVKERRQAAAANARGDFLDQALRDIGSEQFL-SEEFVVNLLFGIL-FASGSVSG 306
           + + KD+   R+  A+    GDFLD  + +   E  + +EE  +NL+F IL  A  S S 
Sbjct: 244 LQVIKDVFTRRK--ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSS 303

Query: 307 SLTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYK-SMTFTLQVIYETLRLS 366
             +L +K LAEN   + EL  EH A L+ R    + ++WEEY+  MTFT  VI ETLR++
Sbjct: 304 VTSLAIKFLAENHKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMA 363

Query: 367 NALPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSI 426
           N  P + R+ + D+ IKGYTIPAGW + V   A+H N   +++PL+FNPWRW+  +  S 
Sbjct: 364 NMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSG 423

Query: 427 SKNLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYIL 455
           SK    FGGG RQC GA+++R+ ++IF+H L+T Y + L
Sbjct: 424 SKTFMVFGGGVRQCVGAEFARLQISIFIHHLVTTYDFSL 451

[Close]

BLAST of CmoCh08G000350 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.0e-69
Identity = 155/443 (34.99%), Postives = 237/443 (53.50%), Query Frame = 0


Query: 13  AIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPFIKKRVQ 72
           +I A  ++LL    YR         LPPGS+G PLIGET QLI +  T +  PFI +RV 
Sbjct: 12  SIAAGFLLLLRRTRYRRMG------LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVA 71

Query: 73  KYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTRTTAGGV 132
           +YG +F T L G   + +ADPE N F+   EGK  E  Y  SI  +L +   +     G 
Sbjct: 72  RYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGK--HSLLLMKGS 131

Query: 133 IHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLYDFNADV 192
           +HK +  +T++   S  +K  L+ DI R V   L  WSSR  V +      + ++     
Sbjct: 132 LHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSSR--VLLMEEAKKITFELTVKQ 191

Query: 193 MFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLVKERRQA 252
           +  +DP + SES+ +  + + +GF ++PL L  T Y K ++A++K+      +V +RR+ 
Sbjct: 192 LMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE 251

Query: 253 AAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFAS-GSVSGSLTLMLKLLAENPSV 312
               A     D     + ++   S+E +V+ L  +L A   + S  +TL +K L E P  
Sbjct: 252 EEEGAERK-KDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLA 311

Query: 313 VRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRTIKDLNI 372
           + +L  EHE     + D  S + W +YKSM FT  V+ ETLR++N +  + RR + D+ I
Sbjct: 312 LAQLKEEHEKIRAMKSDSYS-LEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEI 371

Query: 373 KGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNL-QPFGGGSRQCA 432
           KGY IP GW +  +  A+HL+P   KD   FNPWRW+     +   N+  PFGGG R C 
Sbjct: 372 KGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCP 431

Query: 433 GADYSRVFMAIFLHTLLTKYRYI 454
           G + +RV +++FLH L+T + ++
Sbjct: 432 GYELARVALSVFLHRLVTGFSWV 442

[Close]

BLAST of CmoCh08G000350 vs. ExPASy Swiss-Prot
Match: Q50EK5 (Cytochrome P450 720B2 OS=Pinus taeda OX=3352 GN=CYP720B2 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.1e-67
Identity = 152/453 (33.55%), Postives = 240/453 (52.98%), Query Frame = 0


Query: 8   WSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLP-PF 67
           W  GL    +  +LL      W        LPPGS G+PLIGETI      N+   P  F
Sbjct: 14  WLVGLLCLVLGFLLLQLYKLVWGASSRAYKLPPGSTGWPLIGETISFFRGINSTAQPRQF 73

Query: 68  IKKRVQKYGPIFRTSLVGRS-IVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDT 127
           I++R Q+YG IFR++L GRS IVV+ DPE N  +   EG+  +  Y   +  ++ + G  
Sbjct: 74  IQEREQRYGEIFRSNLFGRSRIVVSVDPEFNKHVLQHEGRQFQANYPKPLRNLIGKYG-- 133

Query: 128 RTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAML 187
             +  G + + L G  +N    E L    + DIQ  +   L +W ++  + +Q     ++
Sbjct: 134 LLSVHGDLQRKLHGAAVNLLRFERLSVDFMEDIQNLLHITLAKWEAKRDIHLQEECHQLV 193

Query: 188 YDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDL 247
            +  A  +    P K +E I E+    ++  +AVP+ +PGTKY +  KA++ L+    + 
Sbjct: 194 LNLMAKQLLDLSPSKDTEEICEAFGHFSEALLAVPIKIPGTKYARGFKAREFLIKKIYES 253

Query: 248 VKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSG-SLTLMLKL 307
           +++RRQ   A    D L + L+    E   SEE + + +  +LFA    S  S++  +K 
Sbjct: 254 IEDRRQHPEA-VHNDLLTKLLK----EDSFSEEIIADFILFLLFAGHETSSRSMSFAIKF 313

Query: 308 LAENPSVVRELTAEHEAFLKQRKD-PESPITWEEYKSMTFTLQVIYETLRLSNALPFLLR 367
           L + P  + EL AEH+A LK++ +     + W++Y+S+ FT  VI+ETLR+ N  P + R
Sbjct: 314 LTDCPRALEELKAEHDALLKRKGNLKNQKLNWDDYQSLKFTQCVIHETLRVGNFGPGVFR 373

Query: 368 RTIKDLNIK-GYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWK-DLDQHSISKN--L 427
            T +D+  K G+ IP GWT+ V  +  HL+   H   L F+PWRW+  L    + KN   
Sbjct: 374 ETKEDIKTKGGFVIPRGWTVYVFLTGTHLDEKYHSSALKFDPWRWQPHLQDQELLKNPSF 433

Query: 428 QPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRY 453
            PFGGG+R C G   +++ +A+FLH  +TK+R+
Sbjct: 434 MPFGGGARLCPGMHLAKMELALFLHNFVTKFRW 459

[Close]

BLAST of CmoCh08G000350 vs. ExPASy TrEMBL
Match: A0A6J1H6S4 (cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111461007 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.3e-274
Identity = 480/507 (94.67%), Postives = 480/507 (94.67%), Query Frame = 0


Query: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60
           MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT
Sbjct: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60

Query: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120
           LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK
Sbjct: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120

Query: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180
           QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG
Sbjct: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180

Query: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240
           TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN
Sbjct: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240

Query: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL 300
           MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL
Sbjct: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL 300

Query: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360
           MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF
Sbjct: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360

Query: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420
           LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ
Sbjct: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420

Query: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWR 480
           PFGGGSRQCAGADYSRVFMAIFLHTLLTKY                           SWR
Sbjct: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKY---------------------------SWR 480

Query: 481 KVKGGDISRSPILKFGDGLHISFKAKA 508
           KVKGGDISRSPILKFGDGLHISFKAKA
Sbjct: 481 KVKGGDISRSPILKFGDGLHISFKAKA 480

[Close]

BLAST of CmoCh08G000350 vs. ExPASy TrEMBL
Match: A0A6J1KW76 (cytochrome P450 87A3-like OS=Cucurbita maxima OX=3661 GN=LOC111498129 PE=3 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 8.2e-272
Identity = 476/509 (93.52%), Postives = 480/509 (94.30%), Query Frame = 0


Query: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60
           MELQLSMWSPGLAIGAVV+ILLTHLVYRWRNPKCNG+LPPGSMGFPLIGETIQLIASGNT
Sbjct: 1   MELQLSMWSPGLAIGAVVVILLTHLVYRWRNPKCNGLLPPGSMGFPLIGETIQLIASGNT 60

Query: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120
           LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK
Sbjct: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120

Query: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180
           QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG
Sbjct: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180

Query: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240
           TLAMLYDFNADVMFGYDPEKSSE+ISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN
Sbjct: 181 TLAMLYDFNADVMFGYDPEKSSENISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240

Query: 241 MFKDLVKERRQ--AAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSL 300
           MFKDLVKERRQ  AAAANA+GDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSL
Sbjct: 241 MFKDLVKERRQAAAAAANAQGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSL 300

Query: 301 TLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNAL 360
           TLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNAL
Sbjct: 301 TLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNAL 360

Query: 361 PFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKN 420
           PFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKN
Sbjct: 361 PFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKN 420

Query: 421 LQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCS 480
           LQPFGGGSRQCAGADYSRVFMAIFLHTLLTKY                           S
Sbjct: 421 LQPFGGGSRQCAGADYSRVFMAIFLHTLLTKY---------------------------S 480

Query: 481 WRKVKGGDISRSPILKFGDGLHISFKAKA 508
           WRKVKGGDISRSPILKFGDGLHISFKAKA
Sbjct: 481 WRKVKGGDISRSPILKFGDGLHISFKAKA 482

[Close]

BLAST of CmoCh08G000350 vs. ExPASy TrEMBL
Match: A0A1S3CCK2 (cytochrome P450 87A3-like OS=Cucumis melo OX=3656 GN=LOC103498905 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.9e-236
Identity = 412/510 (80.78%), Postives = 447/510 (87.65%), Query Frame = 0


Query: 1   MELQLSMWSP--GLA-IGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIAS 60
           ME+   M +P  GLA + AVV+I LT+LVYRWRNPKCNGVLPPGSMGFPLIGET+QLIA 
Sbjct: 1   MEVPSIMSTPVTGLAVVAAVVVIGLTYLVYRWRNPKCNGVLPPGSMGFPLIGETLQLIAP 60

Query: 61  GNTLDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISK 120
           G TLDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIY QEG+TVELWYLDSISK
Sbjct: 61  GYTLDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYNQEGRTVELWYLDSISK 120

Query: 121 VLKQDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEM 180
           V KQDG+ +TTAGG IHKYLR ITLNHFGSESLK+KLLADIQR VDKVL+QWS++PSVE+
Sbjct: 121 VFKQDGEAKTTAGGAIHKYLRSITLNHFGSESLKSKLLADIQRYVDKVLIQWSNQPSVEV 180

Query: 181 QHGTLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKK 240
           Q GTL MLYDFNADVMFGYDPEKS+E+ISESLITLADGFM+ P+N+PGTKYNKCLKAQKK
Sbjct: 181 QRGTLTMLYDFNADVMFGYDPEKSNENISESLITLADGFMSFPVNIPGTKYNKCLKAQKK 240

Query: 241 LVNMFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGS 300
           LVNMFK LVKER QA+ A ARGDFLDQALRDI +EQFL+EEFVVNLLFGILFASGSVSGS
Sbjct: 241 LVNMFKTLVKERHQASVAAARGDFLDQALRDIENEQFLTEEFVVNLLFGILFASGSVSGS 300

Query: 301 LTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNA 360
           LTLM KLLAENPSVV ELTAEHE FLKQRKDPESPITWEEYKSMTFTL VIYE  RLSNA
Sbjct: 301 LTLMFKLLAENPSVVEELTAEHEKFLKQRKDPESPITWEEYKSMTFTLYVIYEIFRLSNA 360

Query: 361 LPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISK 420
           +PFLLRRT KD+NI+GYTIPAGWT+MVANSALHLNP THKDPLDFNPWRWKD DQ+S+SK
Sbjct: 361 MPFLLRRTTKDVNIRGYTIPAGWTIMVANSALHLNPETHKDPLDFNPWRWKDQDQYSVSK 420

Query: 421 NLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGC 480
           NLQPFGGG+RQCAGADYSRVFMAIFLHTL+TKY                           
Sbjct: 421 NLQPFGGGTRQCAGADYSRVFMAIFLHTLVTKY--------------------------- 480

Query: 481 SWRKVKGGDISRSPILKFGDGLHISFKAKA 508
           SW+ VKGG++SRSPILKFGDG+H+SF AKA
Sbjct: 481 SWKVVKGGEVSRSPILKFGDGIHVSFSAKA 483

[Close]

BLAST of CmoCh08G000350 vs. ExPASy TrEMBL
Match: K7NBK8 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-2 PE=2 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.7e-235
Identity = 409/507 (80.67%), Postives = 444/507 (87.57%), Query Frame = 0


Query: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60
           ME QL MW+PGLAI AV +I +THL+YRWRNPKCNGVLPPGSMGFPLIGETIQLIA G T
Sbjct: 1   MESQL-MWTPGLAIVAVAVICVTHLIYRWRNPKCNGVLPPGSMGFPLIGETIQLIAPGYT 60

Query: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120
           LDLPPFIKKRVQ+YGPIFRTSL+GR IVVTADPEIN+FIY QEG++VELWYLDSISKV K
Sbjct: 61  LDLPPFIKKRVQRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFK 120

Query: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180
           QDG+ RTTAGGVIHKYLR ITLNHFGSES+K KLL DIQR V+KVL QWS +PSVE+Q G
Sbjct: 121 QDGEARTTAGGVIHKYLRSITLNHFGSESIKQKLLPDIQRYVNKVLHQWSKQPSVEVQRG 180

Query: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240
           TLAMLYDFNA+ MFGYD EKSSE+IS+SLITLADGFMA PLN+PGTKY+KCLKAQKKLVN
Sbjct: 181 TLAMLYDFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVN 240

Query: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL 300
           M K LVKERRQA +  ARGDFLDQALRDI +EQFL+EEFVVNLLFGILFASGSVSGSLTL
Sbjct: 241 MLKGLVKERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTL 300

Query: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360
           M KLLA+NPSVVRELTAEHEAFLKQR+DP+SPITWEEYKSMTFT+QV+ ETLRLSNALPF
Sbjct: 301 MFKLLADNPSVVRELTAEHEAFLKQREDPQSPITWEEYKSMTFTIQVVNETLRLSNALPF 360

Query: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420
           LLRRT+KD+NIKGYTIPAGWTLMVANSALHLNP THKDPL FNPWRWK+ DQ+SISKNLQ
Sbjct: 361 LLRRTVKDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQ 420

Query: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWR 480
           PFGGG+RQCAGADYSRVFMAIFLHTL+TKYR                           W 
Sbjct: 421 PFGGGARQCAGADYSRVFMAIFLHTLVTKYR---------------------------WT 479

Query: 481 KVKGGDISRSPILKFGDGLHISFKAKA 508
           KVKGG+I RSPILKFGDG+HISF  KA
Sbjct: 481 KVKGGEICRSPILKFGDGIHISFSEKA 479

[Close]

BLAST of CmoCh08G000350 vs. ExPASy TrEMBL
Match: A0A0A0KA91 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088710 PE=2 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.7e-232
Identity = 404/509 (79.37%), Postives = 442/509 (86.84%), Query Frame = 0


Query: 1   MELQLSMWSP--GLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASG 60
           MEL   M +P  GL + A+V+I LT+LV+RWRNPKCNGVLPPGSMGFPLIGET+QLIASG
Sbjct: 1   MELPSVMSTPVTGL-VAALVVIGLTYLVHRWRNPKCNGVLPPGSMGFPLIGETLQLIASG 60

Query: 61  NTLDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKV 120
            TLDLPPFIKKRV KYGPIFRTSLVGRSIVVTADPEINSFIY QEG+TVELWYLDSISKV
Sbjct: 61  YTLDLPPFIKKRVHKYGPIFRTSLVGRSIVVTADPEINSFIYNQEGRTVELWYLDSISKV 120

Query: 121 LKQDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQ 180
            KQDG+ +TTAGG IHKYLR ITLNHFGSESLK+KLLADIQR VDKV  QWS+ PSVEMQ
Sbjct: 121 FKQDGEVKTTAGGAIHKYLRSITLNHFGSESLKSKLLADIQRYVDKVFTQWSNHPSVEMQ 180

Query: 181 HGTLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKL 240
            GTL MLYDFNA +MFGYDPEKS+E+ISESLITLADGFM+ P+N+PGTKYNKCLKAQK+L
Sbjct: 181 RGTLTMLYDFNAYIMFGYDPEKSNENISESLITLADGFMSFPVNVPGTKYNKCLKAQKRL 240

Query: 241 VNMFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSL 300
           VN FK LVKERRQA+ A ARGDFLDQALRDI +EQFL+EEFV NLLFG+LFASGS+SGSL
Sbjct: 241 VNTFKALVKERRQASVAAARGDFLDQALRDIENEQFLTEEFVSNLLFGVLFASGSISGSL 300

Query: 301 TLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNAL 360
           TLM KLLAENPSVV+ELTAEHE FLKQRKDP+SPITWEEYKSMTFTL VIYE  RLSNA+
Sbjct: 301 TLMFKLLAENPSVVKELTAEHETFLKQRKDPKSPITWEEYKSMTFTLYVIYEVFRLSNAM 360

Query: 361 PFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKN 420
           PFLLRRT KD+NIKGYTIPAGWT+MVANSALHLNP THKDPLDFNPWRWKD DQ+SISK 
Sbjct: 361 PFLLRRTTKDVNIKGYTIPAGWTIMVANSALHLNPQTHKDPLDFNPWRWKDHDQYSISKT 420

Query: 421 LQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCS 480
           LQPFGGG+RQCAGADY+RVFMAIFLHTL+TKY                           S
Sbjct: 421 LQPFGGGTRQCAGADYTRVFMAIFLHTLVTKY---------------------------S 480

Query: 481 WRKVKGGDISRSPILKFGDGLHISFKAKA 508
           W+KVKGG++SRSPILKFGDG+H+SF AKA
Sbjct: 481 WKKVKGGEVSRSPILKFGDGIHVSFSAKA 481

[Close]

BLAST of CmoCh08G000350 vs. NCBI nr
Match: XP_022960197.1 (cytochrome P450 87A3-like [Cucurbita moschata])

HSP 1 Score: 954.9 bits (2467), Expect = 2.8e-274
Identity = 480/507 (94.67%), Postives = 480/507 (94.67%), Query Frame = 0


Query: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60
           MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT
Sbjct: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60

Query: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120
           LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK
Sbjct: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120

Query: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180
           QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG
Sbjct: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180

Query: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240
           TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN
Sbjct: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240

Query: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL 300
           MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL
Sbjct: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL 300

Query: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360
           MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF
Sbjct: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360

Query: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420
           LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ
Sbjct: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420

Query: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWR 480
           PFGGGSRQCAGADYSRVFMAIFLHTLLTKY                           SWR
Sbjct: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKY---------------------------SWR 480

Query: 481 KVKGGDISRSPILKFGDGLHISFKAKA 508
           KVKGGDISRSPILKFGDGLHISFKAKA
Sbjct: 481 KVKGGDISRSPILKFGDGLHISFKAKA 480

[Close]

BLAST of CmoCh08G000350 vs. NCBI nr
Match: XP_023515014.1 (cytochrome P450 87A3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 954.5 bits (2466), Expect = 3.6e-274
Identity = 479/507 (94.48%), Postives = 480/507 (94.67%), Query Frame = 0


Query: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60
           MELQLSMWSPGLAIGAVV+ILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT
Sbjct: 1   MELQLSMWSPGLAIGAVVVILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60

Query: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120
           LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK
Sbjct: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120

Query: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180
           QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG
Sbjct: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180

Query: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240
           TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN
Sbjct: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240

Query: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL 300
           MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL
Sbjct: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL 300

Query: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360
           MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF
Sbjct: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360

Query: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420
           LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ
Sbjct: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420

Query: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWR 480
           PFGGGSRQCAGADYSRVFMAIFLHTLLTKY                           SWR
Sbjct: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKY---------------------------SWR 480

Query: 481 KVKGGDISRSPILKFGDGLHISFKAKA 508
           KVKGGDISRSPILKFGDGLHISFKAKA
Sbjct: 481 KVKGGDISRSPILKFGDGLHISFKAKA 480

[Close]

BLAST of CmoCh08G000350 vs. NCBI nr
Match: KAG7025242.1 (Cytochrome P450 87A3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 951.8 bits (2459), Expect = 2.4e-273
Identity = 478/507 (94.28%), Postives = 479/507 (94.48%), Query Frame = 0


Query: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60
           MELQLSMWSPGLAIGAVV ILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT
Sbjct: 1   MELQLSMWSPGLAIGAVVTILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60

Query: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120
           LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK
Sbjct: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120

Query: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180
           QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG
Sbjct: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180

Query: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240
           TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN
Sbjct: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240

Query: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTL 300
           MFKDLVKERRQAAAANARGDFLDQALRDIG+EQFLSEEFVVNLLFGILFASGSVSGSLTL
Sbjct: 241 MFKDLVKERRQAAAANARGDFLDQALRDIGTEQFLSEEFVVNLLFGILFASGSVSGSLTL 300

Query: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360
           MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF
Sbjct: 301 MLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPF 360

Query: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420
           LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ
Sbjct: 361 LLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQ 420

Query: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWR 480
           PFGGGSRQCAGADYSRVFMAIFLHTLLTKY                           SWR
Sbjct: 421 PFGGGSRQCAGADYSRVFMAIFLHTLLTKY---------------------------SWR 480

Query: 481 KVKGGDISRSPILKFGDGLHISFKAKA 508
           KVKGGDISRSPILKFGDGLHISFKAKA
Sbjct: 481 KVKGGDISRSPILKFGDGLHISFKAKA 480

[Close]

BLAST of CmoCh08G000350 vs. NCBI nr
Match: KAG6592835.1 (Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 946.0 bits (2444), Expect = 1.3e-271
Identity = 478/511 (93.54%), Postives = 479/511 (93.74%), Query Frame = 0


Query: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60
           MELQLSMWSPGLAIGAVV ILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT
Sbjct: 1   MELQLSMWSPGLAIGAVVTILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60

Query: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120
           LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK
Sbjct: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120

Query: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180
           QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG
Sbjct: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180

Query: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240
           TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN
Sbjct: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240

Query: 241 MFKDLVKERRQ----AAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSG 300
           MFKDLVKERRQ    AAAANARGDFLDQALRDIG+EQFLSEEFVVNLLFGILFASGSVSG
Sbjct: 241 MFKDLVKERRQAAAAAAAANARGDFLDQALRDIGTEQFLSEEFVVNLLFGILFASGSVSG 300

Query: 301 SLTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSN 360
           SLTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSN
Sbjct: 301 SLTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSN 360

Query: 361 ALPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSIS 420
           ALPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSIS
Sbjct: 361 ALPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSIS 420

Query: 421 KNLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVG 480
           KNLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKY                          
Sbjct: 421 KNLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKY-------------------------- 480

Query: 481 CSWRKVKGGDISRSPILKFGDGLHISFKAKA 508
            SWRKVKGGDISRSPILKFGDGLHISFKAKA
Sbjct: 481 -SWRKVKGGDISRSPILKFGDGLHISFKAKA 484

[Close]

BLAST of CmoCh08G000350 vs. NCBI nr
Match: XP_023005010.1 (cytochrome P450 87A3-like [Cucurbita maxima])

HSP 1 Score: 945.7 bits (2443), Expect = 1.7e-271
Identity = 476/509 (93.52%), Postives = 480/509 (94.30%), Query Frame = 0


Query: 1   MELQLSMWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNT 60
           MELQLSMWSPGLAIGAVV+ILLTHLVYRWRNPKCNG+LPPGSMGFPLIGETIQLIASGNT
Sbjct: 1   MELQLSMWSPGLAIGAVVVILLTHLVYRWRNPKCNGLLPPGSMGFPLIGETIQLIASGNT 60

Query: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120
           LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK
Sbjct: 61  LDLPPFIKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLK 120

Query: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180
           QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG
Sbjct: 121 QDGDTRTTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHG 180

Query: 181 TLAMLYDFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240
           TLAMLYDFNADVMFGYDPEKSSE+ISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN
Sbjct: 181 TLAMLYDFNADVMFGYDPEKSSENISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVN 240

Query: 241 MFKDLVKERRQ--AAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSL 300
           MFKDLVKERRQ  AAAANA+GDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSL
Sbjct: 241 MFKDLVKERRQAAAAAANAQGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSL 300

Query: 301 TLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNAL 360
           TLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNAL
Sbjct: 301 TLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNAL 360

Query: 361 PFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKN 420
           PFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKN
Sbjct: 361 PFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKN 420

Query: 421 LQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCS 480
           LQPFGGGSRQCAGADYSRVFMAIFLHTLLTKY                           S
Sbjct: 421 LQPFGGGSRQCAGADYSRVFMAIFLHTLLTKY---------------------------S 480

Query: 481 WRKVKGGDISRSPILKFGDGLHISFKAKA 508
           WRKVKGGDISRSPILKFGDGLHISFKAKA
Sbjct: 481 WRKVKGGDISRSPILKFGDGLHISFKAKA 482

[Close]

BLAST of CmoCh08G000350 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 427.9 bits (1099), Expect = 1.1e-119
Identity = 207/495 (41.82%), Postives = 316/495 (63.84%), Query Frame = 0


Query: 16  AVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPFIKKRVQKYG 75
           ++++I +TH VY WRNPKC G LPPGSMGFPL+GE+IQ      T D+PPFIK+RV+KYG
Sbjct: 9   SLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFIKERVKKYG 68

Query: 76  PIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVL--KQDGDTRTTAGGVI 135
           PIF+T+LVGR ++V+ D +++ F++ QEG+  + WY D+ + +   K  G       G +
Sbjct: 69  PIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLH----GFM 128

Query: 136 HKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLYDFNADVM 195
           +KYL+ + L  FG + LK K+L  ++   +K L  WS++ SVE++  T +M++D  A  +
Sbjct: 129 YKYLKNMVLTLFGHDGLK-KMLPQVEMTANKRLELWSNQDSVELKDATASMIFDLTAKKL 188

Query: 196 FGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLVKERRQAA 255
             +DP+KSSE++  + +    G ++ P ++PGT Y+KCL+ + K + M +++++ERR+  
Sbjct: 189 ISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERRENP 248

Query: 256 AANARGDFLDQALRDIGSE-QFLSEEFVVNLLFGILFAS-GSVSGSLTLMLKLLAENPSV 315
             N   DF D  + +I  E   L+EE  ++L+F +LFAS  + S +LTL +K L+++P V
Sbjct: 249 RKNP-SDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDPEV 308

Query: 316 VRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRTIKDLNI 375
           ++ LT EHE  L+ R+D +S +TWEEYKSMT+T Q I ET RL+N +P + R+ ++D+  
Sbjct: 309 LKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDIKF 368

Query: 376 KGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQPFGGGSRQCAG 435
           K YTIPAGW +MV   A+HLNP  +KDPL FNP RW+     + SK+   FGGG R C G
Sbjct: 369 KDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRFCVG 428

Query: 436 ADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWRKVKGGDISRSP 495
            D++++ MA FLH+L+TKYR                           W ++KGG+I+R+P
Sbjct: 429 TDFTKLQMAAFLHSLVTKYR---------------------------WEEIKGGNITRTP 470

Query: 496 ILKFGDGLHISFKAK 507
            L+F +G H+    K
Sbjct: 489 GLQFPNGYHVKLHKK 470

[Close]

BLAST of CmoCh08G000350 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 420.2 bits (1079), Expect = 2.3e-117
Identity = 206/501 (41.12%), Postives = 316/501 (63.07%), Query Frame = 0


Query: 16  AVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPFIKKRVQ--- 75
           ++++I +TH VY WRNPKC G LPPGSMGFPL+GE+IQ      T D+PPFIK+RV+   
Sbjct: 9   SLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFIKERVKNDV 68

Query: 76  ---KYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVL--KQDGDTRT 135
              +YGPIF+T+LVGR ++V+ D +++ F++ QEG+  + WY D+ + +   K  G    
Sbjct: 69  DMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLH- 128

Query: 136 TAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLYD 195
              G ++KYL+ + L  FG + LK K+L  ++   +K L  WS++ SVE++  T +M++D
Sbjct: 129 ---GFMYKYLKNMVLTLFGHDGLK-KMLPQVEMTANKRLELWSNQDSVELKDATASMIFD 188

Query: 196 FNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLVK 255
             A  +  +DP+KSSE++  + +    G ++ P ++PGT Y+KCL+ + K + M +++++
Sbjct: 189 LTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQ 248

Query: 256 ERRQAAAANARGDFLDQALRDIGSE-QFLSEEFVVNLLFGILFAS-GSVSGSLTLMLKLL 315
           ERR+    N   DF D  + +I  E   L+EE  ++L+F +LFAS  + S +LTL +K L
Sbjct: 249 ERRENPRKNP-SDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFL 308

Query: 316 AENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRT 375
           +++P V++ LT EHE  L+ R+D +S +TWEEYKSMT+T Q I ET RL+N +P + R+ 
Sbjct: 309 SDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKA 368

Query: 376 IKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQPFGGG 435
           ++D+  K YTIPAGW +MV   A+HLNP  +KDPL FNP RW+     + SK+   FGGG
Sbjct: 369 LRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGG 428

Query: 436 SRQCAGADYSRVFMAIFLHTLLTKYRYILRTTRYKFEILSCSILRRVGFVGCSWRKVKGG 495
            R C G D++++ MA FLH+L+TKYR                           W ++KGG
Sbjct: 429 MRFCVGTDFTKLQMAAFLHSLVTKYR---------------------------WEEIKGG 476

Query: 496 DISRSPILKFGDGLHISFKAK 507
           +I+R+P L+F +G H+    K
Sbjct: 489 NITRTPGLQFPNGYHVKLHKK 476

[Close]

BLAST of CmoCh08G000350 vs. TAIR 10
Match: AT5G48000.2 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 306.2 bits (783), Expect = 4.8e-83
Identity = 171/459 (37.25%), Postives = 261/459 (56.86%), Query Frame = 0


Query: 7   MWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPF 66
           +WS  + + AV  ++++  +YRW NPKCNG LPPGSMG P+IGET          ++ PF
Sbjct: 4   VWSAAVWVIAVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPF 63

Query: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126
           +KKR+ KYGP+FRT++ G + VV  +P+I   ++ QE K+    Y ++  K   +  +  
Sbjct: 64  VKKRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGK--ENV 123

Query: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186
               G IHK+++ I+L H GSE+LK K++ +I R   + L   +++ S + +    ++  
Sbjct: 124 FLKHGNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESV-- 183

Query: 187 DFNADVMFGYDPEKSSESISESLITLADGFMAV-------PLNLPG-TKYNKCLKAQKKL 246
                +M    P+  S    E+  TL D  MA+       PL L       K   A++  
Sbjct: 184 -----IMAHLTPKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYA 243

Query: 247 VNMFKDLVKERRQAAAANARGDFLDQALRDIGSEQFL-SEEFVVNLLFGIL-FASGSVSG 306
           + + KD+   R+  A+    GDFLD  + +   E  + +EE  +NL+F IL  A  S S 
Sbjct: 244 LQVIKDVFTRRK--ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSS 303

Query: 307 SLTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYK-SMTFTLQVIYETLRLS 366
             +L +K LAEN   + EL  EH A L+ R    + ++WEEY+  MTFT  VI ETLR++
Sbjct: 304 VTSLAIKFLAENHKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMA 363

Query: 367 NALPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSI 426
           N  P + R+ + D+ IKGYTIPAGW + V   A+H N   +++PL+FNPWRW+  +  S 
Sbjct: 364 NMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSG 423

Query: 427 SKNLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYIL 455
           SK    FGGG RQC GA+++R+ ++IF+H L+T Y + L
Sbjct: 424 SKTFMVFGGGVRQCVGAEFARLQISIFIHHLVTTYDFSL 451

[Close]

BLAST of CmoCh08G000350 vs. TAIR 10
Match: AT5G48000.3 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 306.2 bits (783), Expect = 4.8e-83
Identity = 171/459 (37.25%), Postives = 261/459 (56.86%), Query Frame = 0


Query: 7   MWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPF 66
           +WS  + + AV  ++++  +YRW NPKCNG LPPGSMG P+IGET          ++ PF
Sbjct: 4   VWSAAVWVIAVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPF 63

Query: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126
           +KKR+ KYGP+FRT++ G + VV  +P+I   ++ QE K+    Y ++  K   +  +  
Sbjct: 64  VKKRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGK--ENV 123

Query: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186
               G IHK+++ I+L H GSE+LK K++ +I R   + L   +++ S + +    ++  
Sbjct: 124 FLKHGNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESV-- 183

Query: 187 DFNADVMFGYDPEKSSESISESLITLADGFMAV-------PLNLPG-TKYNKCLKAQKKL 246
                +M    P+  S    E+  TL D  MA+       PL L       K   A++  
Sbjct: 184 -----IMAHLTPKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYA 243

Query: 247 VNMFKDLVKERRQAAAANARGDFLDQALRDIGSEQFL-SEEFVVNLLFGIL-FASGSVSG 306
           + + KD+   R+  A+    GDFLD  + +   E  + +EE  +NL+F IL  A  S S 
Sbjct: 244 LQVIKDVFTRRK--ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSS 303

Query: 307 SLTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYK-SMTFTLQVIYETLRLS 366
             +L +K LAEN   + EL  EH A L+ R    + ++WEEY+  MTFT  VI ETLR++
Sbjct: 304 VTSLAIKFLAENHKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMA 363

Query: 367 NALPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSI 426
           N  P + R+ + D+ IKGYTIPAGW + V   A+H N   +++PL+FNPWRW+  +  S 
Sbjct: 364 NMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSG 423

Query: 427 SKNLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYIL 455
           SK    FGGG RQC GA+++R+ ++IF+H L+T Y + L
Sbjct: 424 SKTFMVFGGGVRQCVGAEFARLQISIFIHHLVTTYDFSL 451

[Close]

BLAST of CmoCh08G000350 vs. TAIR 10
Match: AT5G48000.1 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 306.2 bits (783), Expect = 4.8e-83
Identity = 171/459 (37.25%), Postives = 261/459 (56.86%), Query Frame = 0


Query: 7   MWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPF 66
           +WS  + + AV  ++++  +YRW NPKCNG LPPGSMG P+IGET          ++ PF
Sbjct: 45  VWSAAVWVIAVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPF 104

Query: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126
           +KKR+ KYGP+FRT++ G + VV  +P+I   ++ QE K+    Y ++  K   +  +  
Sbjct: 105 VKKRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGK--ENV 164

Query: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186
               G IHK+++ I+L H GSE+LK K++ +I R   + L   +++ S + +    ++  
Sbjct: 165 FLKHGNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESV-- 224

Query: 187 DFNADVMFGYDPEKSSESISESLITLADGFMAV-------PLNLPG-TKYNKCLKAQKKL 246
                +M    P+  S    E+  TL D  MA+       PL L       K   A++  
Sbjct: 225 -----IMAHLTPKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYA 284

Query: 247 VNMFKDLVKERRQAAAANARGDFLDQALRDIGSEQFL-SEEFVVNLLFGIL-FASGSVSG 306
           + + KD+   R+  A+    GDFLD  + +   E  + +EE  +NL+F IL  A  S S 
Sbjct: 285 LQVIKDVFTRRK--ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSS 344

Query: 307 SLTLMLKLLAENPSVVRELTAEHEAFLKQRKDPESPITWEEYK-SMTFTLQVIYETLRLS 366
             +L +K LAEN   + EL  EH A L+ R    + ++WEEY+  MTFT  VI ETLR++
Sbjct: 345 VTSLAIKFLAENHKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMA 404

Query: 367 NALPFLLRRTIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSI 426
           N  P + R+ + D+ IKGYTIPAGW + V   A+H N   +++PL+FNPWRW+  +  S 
Sbjct: 405 NMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSG 464

Query: 427 SKNLQPFGGGSRQCAGADYSRVFMAIFLHTLLTKYRYIL 455
           SK    FGGG RQC GA+++R+ ++IF+H L+T Y + L
Sbjct: 465 SKTFMVFGGGVRQCVGAEFARLQISIFIHHLVTTYDFSL 492

The following BLAST results are available for this feature:

BLAST of CmoCh08G000350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
K7NBR2	7.2e-140	49.50	Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=...	[more]
Q7XU38	1.2e-115	42.08	Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV...	[more]
Q8L7D5	6.8e-82	37.25	Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3	[more]
Q42569	3.0e-69	34.99	Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1	[more]
Q50EK5	2.1e-67	33.55	Cytochrome P450 720B2 OS=Pinus taeda OX=3352 GN=CYP720B2 PE=2 SV=1	[more]

BLAST of CmoCh08G000350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6S4	1.3e-274	94.67	cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111461007 PE=3 SV=...	[more]
A0A6J1KW76	8.2e-272	93.52	cytochrome P450 87A3-like OS=Cucurbita maxima OX=3661 GN=LOC111498129 PE=3 SV=1	[more]
A0A1S3CCK2	1.9e-236	80.78	cytochrome P450 87A3-like OS=Cucumis melo OX=3656 GN=LOC103498905 PE=3 SV=1	[more]
K7NBK8	2.7e-235	80.67	Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-2 PE=2 SV=1	[more]
A0A0A0KA91	3.7e-232	79.37	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088710 PE=2 SV=1	[more]

BLAST of CmoCh08G000350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960197.1	2.8e-274	94.67	cytochrome P450 87A3-like [Cucurbita moschata]	[more]
XP_023515014.1	3.6e-274	94.48	cytochrome P450 87A3-like [Cucurbita pepo subsp. pepo]	[more]
KAG7025242.1	2.4e-273	94.28	Cytochrome P450 87A3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6592835.1	1.3e-271	93.54	Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023005010.1	1.7e-271	93.52	cytochrome P450 87A3-like [Cucurbita maxima]	[more]

BLAST of CmoCh08G000350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12740.1	1.1e-119	41.82	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G12740.2	2.3e-117	41.12	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT5G48000.2	4.8e-83	37.25	cytochrome P450, family 708, subfamily A, polypeptide 2	[more]
AT5G48000.3	4.8e-83	37.25	cytochrome P450, family 708, subfamily A, polypeptide 2	[more]
AT5G48000.1	4.8e-83	37.25	cytochrome P450, family 708, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 385..409 score: 25.26 coord: 71..90 score: 31.76 coord: 298..324 score: 21.76 coord: 345..363 score: 33.86 coord: 429..452 score: 24.14 coord: 419..429 score: 48.91
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 31..467 e-value: 6.6E-90 score: 304.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 29..458
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 39..454 e-value: 2.7E-51 score: 174.8
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 9..451 e-value: 5.1E-14 score: 49.8
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 15..457
None	No IPR available	PANTHER	PTHR24286:SF90	CYTOCHROME P450 87A3-LIKE	coord: 15..457
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 422..431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G000350.1	CmoCh08G000350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen