CmoCh08G000300 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G000300
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPlant protein of unknown function (DUF247)
LocationCmo_Chr08: 156342 .. 158922 (+)
RNA-Seq ExpressionCmoCh08G000300
SyntenyCmoCh08G000300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTCAGACCCTCTCCCACCGTGAGCCACGCCGCTCCAACCCCTTCTTTCTTCTCCTGCTTTTTGTAGGAGCATTATTCATTAATATCTGACTACTGCTGATAACGATATTGTGATGCATGCAGTCTGCGTGGAAATCAATGCTTAGGACCCAAGGGAAGCGTCGGTTAGCGTCGTCCAGCACCGCTTCCACCATTGAAAATGCTGCTACGTCTTCTAAGTTAATAAGCAAACCCACTATTATTGAATAGTTATAATATGTAGTGCTTGCTAATGAATGGTTGATTGTGGAAAACAGGGGCAGTCCGAGTAGGAGCGAGTACATGAAGGGTGAGTACGCCCCAGTCTACATTGTGTTGGGGTTCGTAGGGATTGTTTTGATGATGGGAACCCACACGGCGAAGCAGCAGCTGCTGCATTCCCCGGTGGTCAATGTGAGCAAGAAGAAGAGGGAGAGCATCTCGGAGGTGGAGGAGCCCGACACCGTCATCAATTCCGCCGACAAGTTTATCAACAAATCCATTCTTAGGAAGGTGGCCCACATCCAGGACAACACCAAGACTGCGTAGTACGGGATCCCTTTTACCAGCTCACTTCAAGTTTACAATATCTATTATTATTGTCATTTAGTATATAGTATGCCCTTTGGTTTCAGGCCCCCTTATGCTGACACTCCCAAAACTGCTGGAGTGGGTGGATCCTGCCCGGCGTTGAACAACCCCTTGTGTGTAAGCATTTGAATGTCTTCATTTCTTTACTTCTGATTCCCACCAATGTGTGTGTGTGTGTGTATATAATGGGCCCTTCTGGTTCTGGATCTCTTCCCTTTGTGGTGTTGGATGGAGCAGACTAGGTCTAGGCATGAGGCCCTCGGTTCTGTGTTTTTTTTTTTTTTTTCATGTATAATAAAACCAATTGGACTTCCCCAAATTGGGACCTTGGAATTGTTTTCATCTATCTTCATCTTCGATTCTCTCTTCTTCTTCCATTCATTTTATCATTGGAGTGAATCTGATTTTAAGACGACAGAGCAAAGTACTCTTAAAACCAAACTTGCACCAATTCTAATGGATCCATTTGGGAGACTCAGTAGAGAAATGTACTAGAGGGTAGAGGCTAATATTTGTAGCTCACACTCATGCATCAAAAGTTGAGTGTAGCCTATTCATGCATGAACCACGAGAGCTTCACCTTCAAGTAGCTTGCAGGGTGTTGTGTTATATAAAAGGAAATCTTATCATTTTCTTGCATGGGAAGCTTACACCACAGGTGACTATTGGATAGTGGAAAAAAGATCTACTTTGGAGGTAATTTGGATGACGTATAGGTTTAAAAAGTGTACTCTAGAATTAGAATTTCGAGCTTTTGCTCAAGGATTTTGCGAACTCTTATGGTTGAAGTTAAGCTTTGATGACTTACATATATAGGGTTTTAGAAAAGGTTTGATAGATGGTAACATTTATGTCCATCATTTTGAAGAAGAGTCTTTGGATGAAGTAAAATTGTTCCCACTACAAAGCATTATTCCTATTCAAGCCAACCACTTCGTTTTCACTTTTAACGAGTTTTTGCTTTGCTTTGTTGGGACTCAAAGAACTGTACCATTAGGATATCATTACCCGTTTGATGTATTTTAGGTGATATGATCAAATTTGCTTAACAAGCAGCTCCTCCAAGATAAATTCCCGAAATATGACTAATTTGAAAAGGTCGATTGAGAGTTGTTTGGAGTGCTATATGGCCTAACGTGGTGCTGTTCTGATAAAACTCTCCATCATTTTAAAAAGTGTTTGGAATAATGTTAAAGATAAAAGAAAAGCAAAAGAAAAAAGAAACGAGACACATTCTTGGATGTCAAGACAAGTTTTATTCATGCAACCAAAACCAAATATTGGTGCTGCCCCTCTATGTTGTTGTATATATCCATGAGGGTTGAGGAATGAAGGTGCAAATTTGGGTAGCAGCAATGGAGAAACAGCCACCGCTGTCCATCAAACTTATTAGAGATGACAATGTGGATAAACCTGACGACATGGTTATTCAGGTGATGGAGTGTCTGAATGAACCTGGGTTGGATTCTCCTACCAACAAACATTCTATCTACAAACTACCATTCTTCATGAGACAAACTCACTCCGAAGCTTTCAACAAAGCTTTCGATCCCCAGGTGGTGTCATTTGGGCCATACCACCATGGGAAGAAACATTTGTCGCCCATGGAACGGCAAAAGGCTAAAGCGTTCAACACTCTTAAAACCAGAGGACTGCCCGTTGAAGCCATTGTGAGGGAGGTGTCCACCATTTTGGATGACCTCTTGGAATCATACGATAGGCTTGAGGAAGAATGGACGCAAGATCCCGGGAAGTTCTTGAAGCTCATGATTGTGGATGGGTGTTTCATGCTGCGATTGTTCTGCGACTGCCCTGCATCTCTAATTAACATGAAGATGGATATAAGGCTGGAAACGCTGCTGCTTGAGAACCAGCTACCCTTGAAGCTTCTGCATAAGCTGCATTCTATAGCAGGCCCTATCTTCATATCAGAAGGCGATGATGAGCGACCACTCCAGGCAACTTAA

mRNA sequence

ATGGCTTTCAGACCCTCTCCCACCTCTGCGTGGAAATCAATGCTTAGGACCCAAGGGAAGCGTCGGTTAGCGTCGTCCAGCACCGCTTCCACCATTGAAAATGCTGCTACGTCTTCTAAGGGCAGTCCGAGTAGGAGCGAGTACATGAAGGGTGAGTACGCCCCAGTCTACATTGTGTTGGGGTTCGTAGGGATTGTTTTGATGATGGGAACCCACACGGCGAAGCAGCAGCTGCTGCATTCCCCGGTGGTCAATGTGAGCAAGAAGAAGAGGGAGAGCATCTCGGAGGTGGAGGAGCCCGACACCGTCATCAATTCCGCCGACAAGTTTATCAACAAATCCATTCTTAGGAAGGTGGCCCACATCCAGGACAACACCAAGACTGCGTATATGCCCTTTGGTTTCAGGCCCCCTTATGCTGACACTCCCAAAACTGCTGGAGTGGGTGGATCCTGCCCGGCGTTGAACAACCCCTTGTGTGTGCAAATTTGGGTAGCAGCAATGGAGAAACAGCCACCGCTGTCCATCAAACTTATTAGAGATGACAATGTGGATAAACCTGACGACATGGTTATTCAGGTGATGGAGTGTCTGAATGAACCTGGGTTGGATTCTCCTACCAACAAACATTCTATCTACAAACTACCATTCTTCATGAGACAAACTCACTCCGAAGCTTTCAACAAAGCTTTCGATCCCCAGGTGGTGTCATTTGGGCCATACCACCATGGGAAGAAACATTTGTCGCCCATGGAACGGCAAAAGGCTAAAGCGTTCAACACTCTTAAAACCAGAGGACTGCCCGTTGAAGCCATTGTGAGGGAGGTGTCCACCATTTTGGATGACCTCTTGGAATCATACGATAGGCTTGAGGAAGAATGGACGCAAGATCCCGGGAAGTTCTTGAAGCTCATGATTGTGGATGGGTGTTTCATGCTGCGATTGTTCTGCGACTGCCCTGCATCTCTAATTAACATGAAGATGGATATAAGGCTGGAAACGCTGCTGCTTGAGAACCAGCTACCCTTGAAGCTTCTGCATAAGCTGCATTCTATAGCAGGCCCTATCTTCATATCAGAAGGCGATGATGAGCGACCACTCCAGGCAACTTAA

Coding sequence (CDS)

ATGGCTTTCAGACCCTCTCCCACCTCTGCGTGGAAATCAATGCTTAGGACCCAAGGGAAGCGTCGGTTAGCGTCGTCCAGCACCGCTTCCACCATTGAAAATGCTGCTACGTCTTCTAAGGGCAGTCCGAGTAGGAGCGAGTACATGAAGGGTGAGTACGCCCCAGTCTACATTGTGTTGGGGTTCGTAGGGATTGTTTTGATGATGGGAACCCACACGGCGAAGCAGCAGCTGCTGCATTCCCCGGTGGTCAATGTGAGCAAGAAGAAGAGGGAGAGCATCTCGGAGGTGGAGGAGCCCGACACCGTCATCAATTCCGCCGACAAGTTTATCAACAAATCCATTCTTAGGAAGGTGGCCCACATCCAGGACAACACCAAGACTGCGTATATGCCCTTTGGTTTCAGGCCCCCTTATGCTGACACTCCCAAAACTGCTGGAGTGGGTGGATCCTGCCCGGCGTTGAACAACCCCTTGTGTGTGCAAATTTGGGTAGCAGCAATGGAGAAACAGCCACCGCTGTCCATCAAACTTATTAGAGATGACAATGTGGATAAACCTGACGACATGGTTATTCAGGTGATGGAGTGTCTGAATGAACCTGGGTTGGATTCTCCTACCAACAAACATTCTATCTACAAACTACCATTCTTCATGAGACAAACTCACTCCGAAGCTTTCAACAAAGCTTTCGATCCCCAGGTGGTGTCATTTGGGCCATACCACCATGGGAAGAAACATTTGTCGCCCATGGAACGGCAAAAGGCTAAAGCGTTCAACACTCTTAAAACCAGAGGACTGCCCGTTGAAGCCATTGTGAGGGAGGTGTCCACCATTTTGGATGACCTCTTGGAATCATACGATAGGCTTGAGGAAGAATGGACGCAAGATCCCGGGAAGTTCTTGAAGCTCATGATTGTGGATGGGTGTTTCATGCTGCGATTGTTCTGCGACTGCCCTGCATCTCTAATTAACATGAAGATGGATATAAGGCTGGAAACGCTGCTGCTTGAGAACCAGCTACCCTTGAAGCTTCTGCATAAGCTGCATTCTATAGCAGGCCCTATCTTCATATCAGAAGGCGATGATGAGCGACCACTCCAGGCAACTTAA

Protein sequence

MAFRPSPTSAWKSMLRTQGKRRLASSSTASTIENAATSSKGSPSRSEYMKGEYAPVYIVLGFVGIVLMMGTHTAKQQLLHSPVVNVSKKKRESISEVEEPDTVINSADKFINKSILRKVAHIQDNTKTAYMPFGFRPPYADTPKTAGVGGSCPALNNPLCVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQLPLKLLHKLHSIAGPIFISEGDDERPLQAT
Homology
BLAST of CmoCh08G000300 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 7.3e-09
Identity = 41/136 (30.15%), Postives = 71/136 (52.21%), Query Frame = 0

Query: 229 KAFDPQVVSFGPYHHGKKHLSPMERQKAKA----FNTLKTRGLPVEAIVREVSTILDDLL 288
           KA+ P+VVS GPYH+G+KHL  +++ K +      +  K + +    +V+ V  + D + 
Sbjct: 61  KAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEENVLVKAVVDLEDKIR 120

Query: 289 ESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFC----------DCPASLINMKMDIRLET 348
           +SY     E  +     + +M++DGCF+L +F           D   S+  +   I+ + 
Sbjct: 121 KSY----SEELKTGHDLMFMMVLDGCFILMVFLIMSGNIELSEDPIFSIPWLLSSIQSDL 180

Query: 349 LLLENQLPLKLLHKLH 351
           LLLENQ+P  +L  L+
Sbjct: 181 LLLENQVPFFVLQTLY 192

BLAST of CmoCh08G000300 vs. ExPASy TrEMBL
Match: A0A6J1H6V9 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461034 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 5.5e-116
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0

Query: 161 VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 220
           VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR
Sbjct: 3   VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 62

Query: 221 QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 280
           QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL
Sbjct: 63  QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 122

Query: 281 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ 340
           DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ
Sbjct: 123 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ 182

Query: 341 LPLKLLHKLHSIAGPIFISEGDDERPLQ 369
           LPLKLLHKLHSIAGPIFISEGDDERPLQ
Sbjct: 183 LPLKLLHKLHSIAGPIFISEGDDERPLQ 210

BLAST of CmoCh08G000300 vs. ExPASy TrEMBL
Match: A0A6J1KTE2 (uncharacterized protein LOC111498069 OS=Cucurbita maxima OX=3661 GN=LOC111498069 PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 4.3e-97
Identity = 177/200 (88.50%), Postives = 190/200 (95.00%), Query Frame = 0

Query: 161 VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 220
           VQIWVAAMEKQPPLSIK+IRDDNVD+PDDMVIQVMECLNEPGLDSP  KHSIYKLPFFMR
Sbjct: 3   VQIWVAAMEKQPPLSIKIIRDDNVDQPDDMVIQVMECLNEPGLDSPAIKHSIYKLPFFMR 62

Query: 221 QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 280
           +THSEAFN+AFDPQVVSFGPYHHGK+HLSPMER+KAK F++LKT GLPVEAIV  VSTIL
Sbjct: 63  RTHSEAFNQAFDPQVVSFGPYHHGKQHLSPMEREKAKVFDSLKTGGLPVEAIVSCVSTIL 122

Query: 281 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ 340
            DLLESYDRLEEEWTQ PGKFLKLMIVDGCFML+LFC+CP SL NMKMDIRL+TLLLENQ
Sbjct: 123 YDLLESYDRLEEEWTQHPGKFLKLMIVDGCFMLQLFCNCPTSLSNMKMDIRLDTLLLENQ 182

Query: 341 LPLKLLHKLHSIAGPIFISE 361
           LPLKLLHKL+SIAGPIFIS+
Sbjct: 183 LPLKLLHKLYSIAGPIFISQ 202

BLAST of CmoCh08G000300 vs. ExPASy TrEMBL
Match: A0A6J1KRK6 (uncharacterized protein LOC111497635 OS=Cucurbita maxima OX=3661 GN=LOC111497635 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.7e-56
Identity = 123/128 (96.09%), Postives = 124/128 (96.88%), Query Frame = 0

Query: 1   MAFRPSPTSAWKSMLRTQGKRRLASSSTASTIENAATSSKGSPSRSEYMKGEYAPVYIVL 60
           MAFRPS TSAWKSMLRTQGKRRLASSSTASTIENAATSSKG PSRS YMKGEYAPVYIVL
Sbjct: 1   MAFRPSLTSAWKSMLRTQGKRRLASSSTASTIENAATSSKGGPSRSVYMKGEYAPVYIVL 60

Query: 61  GFVGIVLMMGTHTAKQQLLHSPVVNVSKKKRESISEVEEPDTVINSADKFINKSILRKVA 120
           GFVGIVL+MGTHTAKQQLLHSPVVNVSKKKRESI EVEEPDTVINSADKFINKSILRKVA
Sbjct: 61  GFVGIVLLMGTHTAKQQLLHSPVVNVSKKKRESIPEVEEPDTVINSADKFINKSILRKVA 120

Query: 121 HIQDNTKT 129
           HIQDNTKT
Sbjct: 121 HIQDNTKT 128

BLAST of CmoCh08G000300 vs. ExPASy TrEMBL
Match: A0A6J1H7A1 (uncharacterized protein LOC111461134 OS=Cucurbita moschata OX=3662 GN=LOC111461134 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.8e-55
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0

Query: 9   SAWKSMLRTQGKRRLASSSTASTIENAATSSKGSPSRSEYMKGEYAPVYIVLGFVGIVLM 68
           SAWKSMLRTQGKRRLASSSTASTIENAATSSKGSPSRSEYMKGEYAPVYIVLGFVGIVLM
Sbjct: 3   SAWKSMLRTQGKRRLASSSTASTIENAATSSKGSPSRSEYMKGEYAPVYIVLGFVGIVLM 62

Query: 69  MGTHTAKQQLLHSPVVNVSKKKRESISEVEEPDTVINSADKFINKSILRKVAHIQDNTKT 128
           MGTHTAKQQLLHSPVVNVSKKKRESISEVEEPDTVINSADKFINKSILRKVAHIQDNTKT
Sbjct: 63  MGTHTAKQQLLHSPVVNVSKKKRESISEVEEPDTVINSADKFINKSILRKVAHIQDNTKT 122

Query: 129 A 130
           A
Sbjct: 123 A 123

BLAST of CmoCh08G000300 vs. ExPASy TrEMBL
Match: A0A6J1KYV8 (UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497633 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.6e-54
Identity = 121/198 (61.11%), Postives = 146/198 (73.74%), Query Frame = 0

Query: 168 MEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSP----------TNKHSIYKLPF 227
           MEKQPPL + + RDDN+    DMV++V   L++  LDSP          +   SIYK+PF
Sbjct: 1   MEKQPPLQVMVGRDDNIGDKADMVVRVKGTLDQL-LDSPAILNMEAEQSSELLSIYKIPF 60

Query: 228 FMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRG-LPVEAIVREV 287
           FM QTH     KA++PQVVS GPY+HGK+HLSPME +K K F++ KTR  L VE+IVR V
Sbjct: 61  FMTQTHP----KAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKTRCLLDVESIVRGV 120

Query: 288 STILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLL 347
           STILD+L+ESYDRLEEEWTQDPGKFL+LMIVDGCFML     CP SLIN+  DI+ + LL
Sbjct: 121 STILDELMESYDRLEEEWTQDPGKFLQLMIVDGCFMLGFLISCPNSLINVSPDIKQDMLL 180

Query: 348 LENQLPLKLLHKLHSIAG 355
           LENQLP+ LL KL+SIAG
Sbjct: 181 LENQLPMLLLEKLYSIAG 193

BLAST of CmoCh08G000300 vs. NCBI nr
Match: XP_022960231.1 (UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022960232.1 UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 427.6 bits (1098), Expect = 1.1e-115
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0

Query: 161 VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 220
           VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR
Sbjct: 3   VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 62

Query: 221 QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 280
           QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL
Sbjct: 63  QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 122

Query: 281 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ 340
           DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ
Sbjct: 123 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ 182

Query: 341 LPLKLLHKLHSIAGPIFISEGDDERPLQ 369
           LPLKLLHKLHSIAGPIFISEGDDERPLQ
Sbjct: 183 LPLKLLHKLHSIAGPIFISEGDDERPLQ 210

BLAST of CmoCh08G000300 vs. NCBI nr
Match: KAG6592830.1 (UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 425.2 bits (1092), Expect = 5.6e-115
Identity = 206/208 (99.04%), Postives = 207/208 (99.52%), Query Frame = 0

Query: 161 VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 220
           VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSP NKHSIYKLPFFMR
Sbjct: 3   VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFFMR 62

Query: 221 QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 280
           QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL
Sbjct: 63  QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 122

Query: 281 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ 340
           DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL+NMKMDIRLETLLLENQ
Sbjct: 123 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLENQ 182

Query: 341 LPLKLLHKLHSIAGPIFISEGDDERPLQ 369
           LPLKLLHKLHSIAGPIFISEGDDERPLQ
Sbjct: 183 LPLKLLHKLHSIAGPIFISEGDDERPLQ 210

BLAST of CmoCh08G000300 vs. NCBI nr
Match: XP_023004891.1 (uncharacterized protein LOC111498069 [Cucurbita maxima])

HSP 1 Score: 364.8 bits (935), Expect = 9.0e-97
Identity = 177/200 (88.50%), Postives = 190/200 (95.00%), Query Frame = 0

Query: 161 VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 220
           VQIWVAAMEKQPPLSIK+IRDDNVD+PDDMVIQVMECLNEPGLDSP  KHSIYKLPFFMR
Sbjct: 3   VQIWVAAMEKQPPLSIKIIRDDNVDQPDDMVIQVMECLNEPGLDSPAIKHSIYKLPFFMR 62

Query: 221 QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 280
           +THSEAFN+AFDPQVVSFGPYHHGK+HLSPMER+KAK F++LKT GLPVEAIV  VSTIL
Sbjct: 63  RTHSEAFNQAFDPQVVSFGPYHHGKQHLSPMEREKAKVFDSLKTGGLPVEAIVSCVSTIL 122

Query: 281 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ 340
            DLLESYDRLEEEWTQ PGKFLKLMIVDGCFML+LFC+CP SL NMKMDIRL+TLLLENQ
Sbjct: 123 YDLLESYDRLEEEWTQHPGKFLKLMIVDGCFMLQLFCNCPTSLSNMKMDIRLDTLLLENQ 182

Query: 341 LPLKLLHKLHSIAGPIFISE 361
           LPLKLLHKL+SIAGPIFIS+
Sbjct: 183 LPLKLLHKLYSIAGPIFISQ 202

BLAST of CmoCh08G000300 vs. NCBI nr
Match: XP_023513985.1 (UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 346.7 bits (888), Expect = 2.5e-91
Identity = 170/205 (82.93%), Postives = 183/205 (89.27%), Query Frame = 0

Query: 161 VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 220
           VQIWVAAMEKQPPLSIKLIRDDNVDKP DMVIQVM CLNEPGLDSP   H IYKLPFFMR
Sbjct: 3   VQIWVAAMEKQPPLSIKLIRDDNVDKP-DMVIQVMGCLNEPGLDSPAITHCIYKLPFFMR 62

Query: 221 QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 280
           QTH EAFN AFDPQVVSFGPYH GK HLSPMER+KAK FN+LKTRGLPVEA+V  V +IL
Sbjct: 63  QTHPEAFNTAFDPQVVSFGPYHQGKHHLSPMEREKAKVFNSLKTRGLPVEAVVSGVHSIL 122

Query: 281 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLENQ 340
           DDLL SYDRL++EWTQ PGKFL LMIVDGCF+L L C+CPASLINMKMDIR ETLLLENQ
Sbjct: 123 DDLLGSYDRLDQEWTQHPGKFLMLMIVDGCFLLELLCNCPASLINMKMDIRQETLLLENQ 182

Query: 341 LPLKLLHKLHSIAGPIFISEGDDER 366
           LP+KLLH+L+SIAGPIFIS+ D E+
Sbjct: 183 LPMKLLHQLYSIAGPIFISQRDKEQ 206

BLAST of CmoCh08G000300 vs. NCBI nr
Match: KAG7025238.1 (UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 331.3 bits (848), Expect = 1.1e-86
Identity = 160/163 (98.16%), Postives = 160/163 (98.16%), Query Frame = 0

Query: 161 VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFFMR 220
           VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNE GLDSP NKHSIYKLPFFMR
Sbjct: 3   VQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNELGLDSPANKHSIYKLPFFMR 62

Query: 221 QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTIL 280
           QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVR VSTIL
Sbjct: 63  QTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVRGVSTIL 122

Query: 281 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL 324
           DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL
Sbjct: 123 DDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL 165

BLAST of CmoCh08G000300 vs. TAIR 10
Match: AT1G63060.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: petal, leaf whorl, male gametophyte, flower; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, 4 anthesis, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G07330.1); Has 42 Blast hits to 42 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 42; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 91.3 bits (225), Expect = 1.8e-18
Identity = 59/146 (40.41%), Postives = 88/146 (60.27%), Query Frame = 0

Query: 1   MAFRPSPTSAWKSMLRTQGKRRLASSSTASTIENAATSSKGSPSRSEYMKGEYAPVYIVL 60
           MAFR +      S LR  G +   S   A+T+ + A  +    S    + GE+APV I+ 
Sbjct: 1   MAFRIT------SQLRPVGNQFGGSRRFATTVADGAKQTYNKFS----IMGEFAPVAIIG 60

Query: 61  GFVGIVLMMGTHTAKQQLLHSPVVNVSKKKRESISEVEEPDTVINSADKFINKSILRKVA 120
           GFVG+ ++M  H+ KQQL+H+P ++  K +R +++EV++P+  ++SADKFINKS LRKV 
Sbjct: 61  GFVGLAVLMAGHSLKQQLMHAPAISTRKNRRAAVAEVDDPENCVSSADKFINKSWLRKVG 120

Query: 121 HIQDNTKTAYMPFGFRPPYADTPKTA 147
            IQD +K A +    RP    TP+ A
Sbjct: 121 QIQDKSK-AILSDPTRPNPFTTPRNA 135

BLAST of CmoCh08G000300 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 86.3 bits (212), Expect = 5.7e-17
Identity = 58/199 (29.15%), Postives = 101/199 (50.75%), Query Frame = 0

Query: 169 EKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPT---NKHSIYKLPFFMRQTHSE 228
           + Q   +I +I++   D  DD VI + + L +   D  T    K  IY++P+++++    
Sbjct: 59  QTQSDPAIYVIKESPKDSRDDWVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQEND-- 118

Query: 229 AFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFN-TLKTRGLPVEAIVREVSTILDDLL 288
             NK++ PQ VS GPYHHGKK L  M+R K +A N  LK     ++  +  +  + +   
Sbjct: 119 --NKSYFPQTVSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIKMYIDAMRELEEKAR 178

Query: 289 ESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLF--------------CDCPASLINMKMDI 348
             Y   E   +    +F++++++DGCF+L LF               D   ++      I
Sbjct: 179 ACY---EGPLSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYARNDPVFAMRGSMHSI 238

Query: 349 RLETLLLENQLPLKLLHKL 350
           + + ++LENQLPL +L++L
Sbjct: 239 QRDMVMLENQLPLFVLNRL 250

BLAST of CmoCh08G000300 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 79.3 bits (194), Expect = 7.0e-15
Identity = 55/180 (30.56%), Postives = 88/180 (48.89%), Query Frame = 0

Query: 188 DDMVIQVMECLNEPGLDSPT---NKHSIYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHG 247
           D  VI + + L +   D  T    K  IY++P ++++       K++ PQ VS GPYHHG
Sbjct: 88  DSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQEND----KKSYFPQTVSLGPYHHG 147

Query: 248 KKHLSPMERQKAKAFN-TLKTRGLPVEAIVREVSTILDDLLESYDRLEEEWTQDPGKFLK 307
           KK L PMER K +A N  LK     +E     +  + +     Y   E   +    +F +
Sbjct: 148 KKRLRPMERHKWRALNKVLKRLKQRIEMYTNAMRELEEKARACY---EGPISLSRNEFTE 207

Query: 308 LMIVDGCFMLRLF--------------CDCPASLINMKMDIRLETLLLENQLPLKLLHKL 350
           ++++DGCF+L LF               D   ++  +   I+ + ++LENQLPL +L +L
Sbjct: 208 MLVLDGCFVLELFRGTVEGFTEIGYARNDPVFAMRGLMHSIQRDMIMLENQLPLFVLDRL 260

BLAST of CmoCh08G000300 vs. TAIR 10
Match: AT3G50170.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 79.3 bits (194), Expect = 7.0e-15
Identity = 55/180 (30.56%), Postives = 88/180 (48.89%), Query Frame = 0

Query: 188 DDMVIQVMECLNEPGLDSPT---NKHSIYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHG 247
           D  VI + + L +   D  T    K  IY++P ++++       K++ PQ VS GPYHHG
Sbjct: 88  DSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQEND----KKSYFPQTVSLGPYHHG 147

Query: 248 KKHLSPMERQKAKAFN-TLKTRGLPVEAIVREVSTILDDLLESYDRLEEEWTQDPGKFLK 307
           KK L PMER K +A N  LK     +E     +  + +     Y   E   +    +F +
Sbjct: 148 KKRLRPMERHKWRALNKVLKRLKQRIEMYTNAMRELEEKARACY---EGPISLSRNEFTE 207

Query: 308 LMIVDGCFMLRLF--------------CDCPASLINMKMDIRLETLLLENQLPLKLLHKL 350
           ++++DGCF+L LF               D   ++  +   I+ + ++LENQLPL +L +L
Sbjct: 208 MLVLDGCFVLELFRGTVEGFTEIGYARNDPVFAMRGLMHSIQRDMIMLENQLPLFVLDRL 260

BLAST of CmoCh08G000300 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 79.0 bits (193), Expect = 9.2e-15
Identity = 53/186 (28.49%), Postives = 92/186 (49.46%), Query Frame = 0

Query: 188 DDMVIQVMECLNEPGLDSPT---NKHSIYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHG 247
           ++ VI + + + +   +  T   +K  IY++P ++++ +     K++ PQ VS GP+HHG
Sbjct: 114 EEWVISIRDKMEQALREDATTSWDKLCIYRVPQYLQENN----KKSYFPQTVSLGPFHHG 173

Query: 248 KKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTILDDLLESYDR----LEEEWTQDPGK 307
            KHL PM+R K +A N +  R         ++   +D + E  DR     E        K
Sbjct: 174 NKHLLPMDRHKWRAVNMVMAR------TKHDIEMYIDAMKELEDRARACYEGPIDLSSNK 233

Query: 308 FLKLMIVDGCFMLRLFCDCPASLINMKMD--------------IRLETLLLENQLPLKLL 353
           F +++++DGCF+L LF         +  D              I+ + ++LENQLPL +L
Sbjct: 234 FSEMLVLDGCFVLELFRGADEGFSELGYDRNDPVFAMRGSMHSIQRDMVMLENQLPLFVL 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD537.3e-0930.15UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H6V95.5e-116100.00UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461034 PE=... [more]
A0A6J1KTE24.3e-9788.50uncharacterized protein LOC111498069 OS=Cucurbita maxima OX=3661 GN=LOC111498069... [more]
A0A6J1KRK61.7e-5696.09uncharacterized protein LOC111497635 OS=Cucurbita maxima OX=3661 GN=LOC111497635... [more]
A0A6J1H7A11.8e-55100.00uncharacterized protein LOC111461134 OS=Cucurbita moschata OX=3662 GN=LOC1114611... [more]
A0A6J1KYV81.6e-5461.11UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_022960231.11.1e-115100.00UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022960232.1 UPF0481 prot... [more]
KAG6592830.15.6e-11599.04UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023004891.19.0e-9788.50uncharacterized protein LOC111498069 [Cucurbita maxima][more]
XP_023513985.12.5e-9182.93UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo][more]
KAG7025238.11.1e-8698.16UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G63060.11.8e-1840.41unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G50120.15.7e-1729.15Plant protein of unknown function (DUF247) [more]
AT3G50170.17.0e-1530.56Plant protein of unknown function (DUF247) [more]
AT3G50170.27.0e-1530.56Plant protein of unknown function (DUF247) [more]
AT3G50130.19.2e-1528.49Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 273..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..45
NoneNo IPR availablePANTHERPTHR33919OS09G0127700 PROTEINcoord: 1..145
NoneNo IPR availablePANTHERPTHR33919:SF9RIBOSOME BIOGENESIS NEP1-LIKE PROTEINcoord: 1..145
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 212..353
e-value: 4.0E-26
score: 92.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G000300.1CmoCh08G000300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane