CmoCh08G000060 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G000060
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionACT domain-containing protein
LocationCmo_Chr08: 34661 .. 37081 (+)
RNA-Seq ExpressionCmoCh08G000060
SyntenyCmoCh08G000060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAGAGCATTTGCAAAAGGTATTTCACAGAAACCGGGAGACATTGATTTGTTTTGTTTTTCCCCAAATTAAAAACTTGCTCTTCCTCTTCTTCCCCAAATCATGGATTGTTGGTCCCTTTCTCACCCTCTCGACGACGAGTTCGAGAAGCTCGTCAATCGCATGAACCCTCCAAGGTCAATCTTCCCTCTCTTTCCTCTGTTTCTTCTCTCTCTCTCTCTCAACTGCCTTTTCTGTTTCTCCTCTCTCTCAAATGGGTTTCCTTTTCAGGGTCACCATTGATAACGATTCCTGCTCAAACGCCACTCTCATCAAGGTCTTTTTTCAACTTCAATTTCTCTTTCAACATCTCACCCTTTTCGCTTCTCATCGCTTTCACTCCCCAATTTTTCAGGTTCATAGCGCCAATAAGCGAGGGAGTTTGCTGGAAGTGGTTCAGGTTCTTAATGATTTGAATTTCACCATTAGACGGGCTTACATTTCTTCTGATGGCGAGTGGTTCATGGATGGTCAGCTCTCACATTTTATTTTTCTTGCCCTTAATATCCAATTTTCATTCAACTCCAATTCATAACAAAAACAAAAACCAAATTATTCATTGTTTTTTTTTTTTCTCTTTTTTCTTCAGTGTTTCATGTAACGGATCACCGTGGGAACAAGCTCTCTGAAAATGGAGTTGCTGAGCGTATTCAACAGGTGAATTTCAATGCCCACTTTTGCTTGAGCTTACAACTCCATTTCAAATGTTTCCACTTGACTTGTGAGGAACTATGGGAATTGGAAACGGGATTCTTATTCTGGATTAGCCTTTCATACCAATTTTGTGTGATTTTAGTCTCCGCTCCCCTCTTTTTCTACTTTTTCAGCTAATTTTTCATTCAAGGGACAGAAGGTTTGAGAAAGGTGTTGGAAGTTTTATGTATCATCTATAAGAATTTGTAATGACTTCTGTTGGCTGTTGTTTGACTTGTTCTAAGGTTAAGGTTGTATAAGGTGTTATGTTTGTGCTAATGGATGAATTTTATTATTTCAGTCACTCGGGCCAAGGGCTCTGAGTTTCCGTTCGTTGAGGTCGGTCGGTGTTCAAGCTGCTGAGAAACATACAACCATTGAATTGACTGGAAGAGATAGGCCTGGTTTGCTCTCTGAGGTTTTTGCTGTTCTAGCAGACCTCAAATGCAATGTGGTAGCTGCAGAAGTTTGGACTCATAATTCGAGAATGGCTTCCGTTGTGTACATCACTGACGAGGCTACTGGCCTGCCACTTGATAATACCGACCGGCTTGGTAAGATAAAGCGGCTTCTCCTTTACATCTTGAAAGGGAATCGGGATAAGCGCAGTGCCAACACTGCTGTTTCTGTGTGTTCTACTCACAAGGAAAGGAGGCTGCATCAGATGATGTATGAAGACCGTGATTACGACCGAGATGACTTGGACTGTGGCTCAACAAGTGATCACAGAAAACCCCTTGTAATTGTAGAGAACTGTACCGATAAGGGATATACCGTTGTGAACTTGAGGTCTCCCGACTGTCCAAAGTTGCTGTTTGATACCGTTTGCACGCTAACAGATATGCAGTATGTAGTGTACCATGCTACTGTCATTGCTGAAGGACCAGAGGCTACTCAGGTAACTTCTTATCCGTTCATCGCCATTGTCGATGTTTCGTGATGCATTTGGTGTAACTCATTGGACTAAGCATTTGTGGAGCAGGAGTACTATATCAGGCATATGGATGGAAGTCCTATTAGTTCTGAAGCAGAGAGGCAAAGAGTAATCCATTGCTTAGAGGCTGCCATTCGAAGGAGAAACTCCGAGGTGATAACCGATATTGATTTACATTTCTTGCCTAAGATTTGTGTTCTCGATTGGAGTCTTGTGTGATTTTACTCTGCCAAATACTCAGGGTATAAGACTAGAACTTTGCAGTGAGGATAGGGCTGGACTCCTTTCCGATGTGACTCGAATCTTTAGAGAAAACGGTCTTTCAGTCACTCGAGCTGATGTTACCACTCGCGGTTCTCAAGCTGTCAATGTGTTCTATGTAACTGATTCATCTGGGAGTCCAGTCAAGATACAGATGATTGAAGCAATTCGAAAAGAGATTGGCCTCACTATACTCCATGTCATAGACGACGAATCCTGCACAAAATCTCCATCTCCAGAAAGCAGTAGATTTTCGCTCGGTAATCTTTTTCGATCAAGATCAGGGAAGTTTCTTTATAACTTGGGCTTGATAAACTCGTGTTCTTGAGGAAATTGGGTTGGTAAAATGTTAGAAATGTCCATGTGAAGGAATGGGCTTAGAGAGTGCTCAAAATTGTAACACAGTTTATGATATGAACCAAGAGACATCAATCATACAATATGCTTCAATGAAGATGTGAAGAAAAGATTGTCAAAATTATCAAAAGATGTT

mRNA sequence

AGAGAGAGCATTTGCAAAAGGTATTTCACAGAAACCGGGAGACATTGATTTGTTTTGTTTTTCCCCAAATTAAAAACTTGCTCTTCCTCTTCTTCCCCAAATCATGGATTGTTGGTCCCTTTCTCACCCTCTCGACGACGAGTTCGAGAAGCTCGTCAATCGCATGAACCCTCCAAGGGTCACCATTGATAACGATTCCTGCTCAAACGCCACTCTCATCAAGGTTCATAGCGCCAATAAGCGAGGGAGTTTGCTGGAAGTGGTTCAGGTTCTTAATGATTTGAATTTCACCATTAGACGGGCTTACATTTCTTCTGATGGCGAGTGGTTCATGGATGTGTTTCATGTAACGGATCACCGTGGGAACAAGCTCTCTGAAAATGGAGTTGCTGAGCGTATTCAACAGTCACTCGGGCCAAGGGCTCTGAGTTTCCGTTCGTTGAGGTCGGTCGGTGTTCAAGCTGCTGAGAAACATACAACCATTGAATTGACTGGAAGAGATAGGCCTGGTTTGCTCTCTGAGGTTTTTGCTGTTCTAGCAGACCTCAAATGCAATGTGGTAGCTGCAGAAGTTTGGACTCATAATTCGAGAATGGCTTCCGTTGTGTACATCACTGACGAGGCTACTGGCCTGCCACTTGATAATACCGACCGGCTTGGTAAGATAAAGCGGCTTCTCCTTTACATCTTGAAAGGGAATCGGGATAAGCGCAGTGCCAACACTGCTGTTTCTGTGTGTTCTACTCACAAGGAAAGGAGGCTGCATCAGATGATGTATGAAGACCGTGATTACGACCGAGATGACTTGGACTGTGGCTCAACAAGTGATCACAGAAAACCCCTTGTAATTGTAGAGAACTGTACCGATAAGGGATATACCGTTGTGAACTTGAGGTCTCCCGACTGTCCAAAGTTGCTGTTTGATACCGTTTGCACGCTAACAGATATGCAGTATGTAGTGTACCATGCTACTGTCATTGCTGAAGGACCAGAGGCTACTCAGGAGTACTATATCAGGCATATGGATGGAAGTCCTATTAGTTCTGAAGCAGAGAGGCAAAGAGTAATCCATTGCTTAGAGGCTGCCATTCGAAGGAGAAACTCCGAGGGTATAAGACTAGAACTTTGCAGTGAGGATAGGGCTGGACTCCTTTCCGATGTGACTCGAATCTTTAGAGAAAACGGTCTTTCAGTCACTCGAGCTGATGTTACCACTCGCGGTTCTCAAGCTGTCAATGTGTTCTATGTAACTGATTCATCTGGGAGTCCAGTCAAGATACAGATGATTGAAGCAATTCGAAAAGAGATTGGCCTCACTATACTCCATGTCATAGACGACGAATCCTGCACAAAATCTCCATCTCCAGAAAGCAGTAGATTTTCGCTCGGTAATCTTTTTCGATCAAGATCAGGGAAGTTTCTTTATAACTTGGGCTTGATAAACTCGTGTTCTTGAGGAAATTGGGTTGGTAAAATGTTAGAAATGTCCATGTGAAGGAATGGGCTTAGAGAGTGCTCAAAATTGTAACACAGTTTATGATATGAACCAAGAGACATCAATCATACAATATGCTTCAATGAAGATGTGAAGAAAAGATTGTCAAAATTATCAAAAGATGTT

Coding sequence (CDS)

ATGGATTGTTGGTCCCTTTCTCACCCTCTCGACGACGAGTTCGAGAAGCTCGTCAATCGCATGAACCCTCCAAGGGTCACCATTGATAACGATTCCTGCTCAAACGCCACTCTCATCAAGGTTCATAGCGCCAATAAGCGAGGGAGTTTGCTGGAAGTGGTTCAGGTTCTTAATGATTTGAATTTCACCATTAGACGGGCTTACATTTCTTCTGATGGCGAGTGGTTCATGGATGTGTTTCATGTAACGGATCACCGTGGGAACAAGCTCTCTGAAAATGGAGTTGCTGAGCGTATTCAACAGTCACTCGGGCCAAGGGCTCTGAGTTTCCGTTCGTTGAGGTCGGTCGGTGTTCAAGCTGCTGAGAAACATACAACCATTGAATTGACTGGAAGAGATAGGCCTGGTTTGCTCTCTGAGGTTTTTGCTGTTCTAGCAGACCTCAAATGCAATGTGGTAGCTGCAGAAGTTTGGACTCATAATTCGAGAATGGCTTCCGTTGTGTACATCACTGACGAGGCTACTGGCCTGCCACTTGATAATACCGACCGGCTTGGTAAGATAAAGCGGCTTCTCCTTTACATCTTGAAAGGGAATCGGGATAAGCGCAGTGCCAACACTGCTGTTTCTGTGTGTTCTACTCACAAGGAAAGGAGGCTGCATCAGATGATGTATGAAGACCGTGATTACGACCGAGATGACTTGGACTGTGGCTCAACAAGTGATCACAGAAAACCCCTTGTAATTGTAGAGAACTGTACCGATAAGGGATATACCGTTGTGAACTTGAGGTCTCCCGACTGTCCAAAGTTGCTGTTTGATACCGTTTGCACGCTAACAGATATGCAGTATGTAGTGTACCATGCTACTGTCATTGCTGAAGGACCAGAGGCTACTCAGGAGTACTATATCAGGCATATGGATGGAAGTCCTATTAGTTCTGAAGCAGAGAGGCAAAGAGTAATCCATTGCTTAGAGGCTGCCATTCGAAGGAGAAACTCCGAGGGTATAAGACTAGAACTTTGCAGTGAGGATAGGGCTGGACTCCTTTCCGATGTGACTCGAATCTTTAGAGAAAACGGTCTTTCAGTCACTCGAGCTGATGTTACCACTCGCGGTTCTCAAGCTGTCAATGTGTTCTATGTAACTGATTCATCTGGGAGTCCAGTCAAGATACAGATGATTGAAGCAATTCGAAAAGAGATTGGCCTCACTATACTCCATGTCATAGACGACGAATCCTGCACAAAATCTCCATCTCCAGAAAGCAGTAGATTTTCGCTCGGTAATCTTTTTCGATCAAGATCAGGGAAGTTTCTTTATAACTTGGGCTTGATAAACTCGTGTTCTTGA

Protein sequence

MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQAAEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLDNTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGSTSDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRENGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPSPESSRFSLGNLFRSRSGKFLYNLGLINSCS
Homology
BLAST of CmoCh08G000060 vs. ExPASy Swiss-Prot
Match: Q8LJW3 (ACT domain-containing protein ACR4 OS=Arabidopsis thaliana OX=3702 GN=ACR4 PE=2 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.4e-139
Identity = 261/453 (57.62%), Postives = 331/453 (73.07%), Query Frame = 0

Query: 5   SLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNFTI 64
           S S  +D+E+EKL+ RMNPPRV IDNDSC  AT+I+V SAN+ G LLEVVQ+L DLN TI
Sbjct: 2   SFSQDMDNEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTI 61

Query: 65  RRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQAAEKH 124
            +AYISSDG WFMDVF+VTD  GNK+++  V + IQ+SLGP A    S+RSVGV  +   
Sbjct: 62  TKAYISSDGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDS 121

Query: 125 TTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLDNTDR 184
           T IELTG DRPGLLSE+ AVL  LKC+V+ AE+WTHN+R A+V+ +TD+ TG  + + +R
Sbjct: 122 TVIELTGCDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPER 181

Query: 185 LGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRD--DLDCGSTSD 244
           L +IK LL  +LKG+   R A T VS    H +RRLHQMM+EDRDY+    D D     +
Sbjct: 182 LSRIKNLLRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDDSSIQDE 241

Query: 245 HRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQEY 304
            ++P V V+N  DK Y+VV +R  D PKLLFDTVCTLTDMQYVV+H +V  EG EA QEY
Sbjct: 242 RQRPDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEY 301

Query: 305 YIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRENGL 364
           Y+RH+DGSP+ SEAE+QRVI CLEAAI+RR SEG++LELC+ DR GLLS+VTRIFREN L
Sbjct: 302 YVRHIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSL 361

Query: 365 SVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHV----IDDESCTKS 424
           +VTRA+V T+G +A+N FYV+D+SG  +  + I++IR+ IG TIL V     + +   KS
Sbjct: 362 TVTRAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQKS 421

Query: 425 PSPES-SRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PS ES +RF  G LF+S+S     N GL+ S S
Sbjct: 422 PSHESPTRFLFGGLFKSKS---FVNFGLVRSYS 451

BLAST of CmoCh08G000060 vs. ExPASy Swiss-Prot
Match: Q9ZPQ8 (ACT domain-containing protein ACR5 OS=Arabidopsis thaliana OX=3702 GN=ACR5 PE=2 SV=2)

HSP 1 Score: 453.0 bits (1164), Expect = 4.0e-126
Identity = 256/461 (55.53%), Postives = 323/461 (70.07%), Query Frame = 0

Query: 3   CWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNF 62
           C S S+ +DDE  K + R+NPPRV IDN+ C + T+IKV SANK G LLEVVQVL +LN 
Sbjct: 4   CLSYSYNMDDEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNL 63

Query: 63  TIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALS--FRSLRS-VGVQ 122
           TI++AYISSDG WFMDVF+VTD  GNK+++  V E I++SLGP   S    S+RS +GV+
Sbjct: 64  TIKKAYISSDGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVK 123

Query: 123 AAEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPL 182
            +  +T +ELTG DRPGLLSE+ AVL DL+CNVV AE+WTH ++ A+V+ +TDE T   +
Sbjct: 124 QSVDYTVVELTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAI 183

Query: 183 DNTDRLGKIKRLLLYILKG---NRDKRSANTAVS--VCSTHKERRLHQMMYEDRDYDR-- 242
            + +RL KI++LL Y+L G    R  R   T VS  +  TH +R+LHQ+M+ DRDYD   
Sbjct: 184 TDPERLSKIRKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDEWE 243

Query: 243 ---DDLD-CGSTSDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYH 302
              DD D CG       P V V N  D  Y++V ++  D PKLLFDTV TLTDM YVV H
Sbjct: 244 NNVDDEDKCGRVI----PDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSH 303

Query: 303 ATVIAEGPEATQEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAG 362
           A++ AEGP+A QEYYIRH DGSP+ SEAERQRVI CL+AAI+RR SEG++LELC+ DR G
Sbjct: 304 ASIDAEGPQAYQEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVG 363

Query: 363 LLSDVTRIFRENGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILH 422
           LLSDVTRIFREN L+VTRA+V T+G +A+N FYV D+SG  V  + IE+IR+ IG TIL 
Sbjct: 364 LLSDVTRIFRENSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQ 423

Query: 423 VIDDESCTKSPSPESSRFS-LGNLFRSRSGKFLYNLGLINS 449
           V    +  K PSP+ S    L  +F+SRS     N GLI S
Sbjct: 424 VKGGNTDAK-PSPQDSPTGFLFGVFKSRS---FVNFGLIRS 456

BLAST of CmoCh08G000060 vs. ExPASy Swiss-Prot
Match: O49285 (ACT domain-containing protein ACR3 OS=Arabidopsis thaliana OX=3702 GN=ACR3 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.3e-121
Identity = 223/404 (55.20%), Postives = 295/404 (73.02%), Query Frame = 0

Query: 11  DDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNFTIRRAYIS 70
           D E+E L +R+NPP V+IDN SC   TL+KV S NK G LLEVVQVL DL+ TI +AYIS
Sbjct: 10  DPEYENLSSRINPPSVSIDNTSCKECTLVKVDSMNKPGILLEVVQVLTDLDLTITKAYIS 69

Query: 71  SDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSL-----RSVGVQAAEKHT 130
           SDG WFMDVFHVTD +GNK++++   + I++ LGP+  +  S      + VGV +   HT
Sbjct: 70  SDGGWFMDVFHVTDQQGNKVTDSKTIDYIEKVLGPKGHASASQNTWPGKRVGVHSLGDHT 129

Query: 131 TIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLDNTDRL 190
           +IE+  RDRPGLLSEV AVLADL  NVVAAE WTHN R+A V+Y+ D AT   +D+ +RL
Sbjct: 130 SIEIIARDRPGLLSEVSAVLADLNINVVAAEAWTHNRRIACVLYVNDNATSRAVDDPERL 189

Query: 191 GKIKRLLLYILKG--NRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGSTSDH 250
             ++  L  +L+G   +D++ A T++S+ STH +RRLHQM + DRDY+       S S  
Sbjct: 190 SSMEEQLNNVLRGCEEQDEKFARTSLSIGSTHVDRRLHQMFFADRDYEAVTKLDDSASCG 249

Query: 251 RKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQEYY 310
            +P + VE+C +KGY+V+N+   D PKL+FD VCTLTDMQY+V+HAT+ + G  A+QEY+
Sbjct: 250 FEPKITVEHCEEKGYSVINVSCEDRPKLMFDIVCTLTDMQYIVFHATISSSGSHASQEYF 309

Query: 311 IRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRENGLS 370
           IRH DG  + +E E++RV+ CLEAAI RR SEG  LELC++DR GLLS+VTRI RE+GLS
Sbjct: 310 IRHKDGCTLDTEGEKERVVKCLEAAIHRRVSEGWSLELCAKDRVGLLSEVTRILREHGLS 369

Query: 371 VTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTIL 408
           V+RA VTT G QAVNVFYV D+SG+PV ++ IEA+R EIG +++
Sbjct: 370 VSRAGVTTVGEQAVNVFYVKDASGNPVDVKTIEALRGEIGHSMM 413

BLAST of CmoCh08G000060 vs. ExPASy Swiss-Prot
Match: Q9SGA0 (ACT domain-containing protein ACR6 OS=Arabidopsis thaliana OX=3702 GN=ACR6 PE=1 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 8.1e-119
Identity = 231/435 (53.10%), Postives = 308/435 (70.80%), Query Frame = 0

Query: 11  DDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNFTIRRAYIS 70
           DDE+ KL+ RMNPPRV IDN++  +AT+I+V S NK G+LLEVVQVL D+N  I++AYIS
Sbjct: 3   DDEYAKLIRRMNPPRVVIDNNASDDATVIQVDSVNKHGTLLEVVQVLTDMNLVIKKAYIS 62

Query: 71  SDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSF-RSLR-SVGVQAAEKHTTIE 130
           SDG WFMDVF V D  GNK+ +  V + IQ+ +   A  F   LR SVGV   +++T+IE
Sbjct: 63  SDGGWFMDVFKVIDQDGNKIRDTQVLDYIQKRIESNAGWFIPPLRSSVGVMPTDEYTSIE 122

Query: 131 LTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLDNTDRLGKI 190
           L G DRPGLLSEV AVL DL CNVV AE+WTHN+R A+V+++TD +T   + +  RL  I
Sbjct: 123 LAGTDRPGLLSEVSAVLTDLHCNVVNAEIWTHNTRAAAVIHVTDNSTHSAITDPIRLSTI 182

Query: 191 KRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGSTSDHRKPLV 250
           K LL  +++ N   R+A T  S   TH+ERRLHQ+M++DRDY+       S S   +P V
Sbjct: 183 KELLCNVVRTNSGSRAAKTVFSCSDTHRERRLHQIMFDDRDYEGVKRARTSAS---RPSV 242

Query: 251 IVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQEYYIRHMD 310
            + N  +K YTVV +RS D PKL+FD VCTLTDMQYVV+H  V  E  EA QE+YIRH+D
Sbjct: 243 TLMN-IEKDYTVVTMRSKDRPKLVFDVVCTLTDMQYVVFHGMVSTEPVEAYQEFYIRHVD 302

Query: 311 GSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRENGLSVTRAD 370
           G PI+SEAE++RVI CLEAAI RR SEG+ LEL +EDR GLLSD+TR FREN L++ RA+
Sbjct: 303 GLPINSEAEQERVIQCLEAAIERRASEGLELELSAEDRVGLLSDITRTFRENSLTIVRAE 362

Query: 371 VTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHV--IDDESC----TKSPSPE 430
           ++TR  +A + FYVTD +G+PV+ +++E+IR++IG++ L V   + E C    T  PS E
Sbjct: 363 ISTREGKAKDTFYVTDVTGNPVESKIVESIRQQIGVSKLKVKKKEKEHCSVLGTSRPSHE 422

Query: 431 SSR--FSLGNLFRSR 436
           ++   + L N+F+ +
Sbjct: 423 TTTMGYLLSNIFKPK 433

BLAST of CmoCh08G000060 vs. ExPASy Swiss-Prot
Match: Q9LNA5 (ACT domain-containing protein ACR8 OS=Arabidopsis thaliana OX=3702 GN=ACR8 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 5.1e-113
Identity = 229/450 (50.89%), Postives = 303/450 (67.33%), Query Frame = 0

Query: 12  DEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNFTIRRAYISS 71
           DE+EKLV RMN PRV IDN  CS+AT++KV S+ + G LLE VQ+L DLN +I++AYISS
Sbjct: 8   DEYEKLVIRMNTPRVVIDNGVCSSATIVKVDSSRRNGILLEAVQILTDLNLSIKKAYISS 67

Query: 72  DGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQAAEKHTTIELTG 131
           DG W MDVFHVTD  GNKL++  V   I+QS+        ++   G+      T +ELTG
Sbjct: 68  DGTWNMDVFHVTDLNGNKLNDQSVLRYIEQSI-ETVYYGENIEVNGL------TALELTG 127

Query: 132 RDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLDNTDRLGKIKRL 191
            DR GLLSE+FAVL+DL C+VV A++WTHN R+ASV+Y+ D  +G P+ ++ R+ KI+  
Sbjct: 128 TDRIGLLSEMFAVLSDLNCDVVDAKLWTHNGRVASVIYLKDCISGAPILDSHRISKIEGR 187

Query: 192 LLYILKGNRDKRS-ANTAVSVCS-THKERRLHQMMYEDRDYDRDDLDCGSTSDHRKPLVI 251
           L  +L G+ D  S A T V+V S  H ERRLHQ+M+EDRDY+R      S    R P+V+
Sbjct: 188 LKNVLNGDNDVNSAAKTCVTVDSMMHIERRLHQLMFEDRDYER-----RSKKHERSPMVV 247

Query: 252 --VENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQEYYIRHM 311
             V+N  ++GY+VVN+   D  KLLFD VCTLTDM+Y V+HAT+     +A  E+YIRH 
Sbjct: 248 VTVQNWAERGYSVVNVHCRDRTKLLFDVVCTLTDMEYAVFHATINTAEDQAHLEFYIRHK 307

Query: 312 DGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRENGLSVTRA 371
           DGSPISSEAERQRVI CLEAA+ RR  EG+RLEL   D+ GLL++VTR FRENGL+VTR 
Sbjct: 308 DGSPISSEAERQRVIQCLEAAVERRALEGVRLELRHPDKQGLLAEVTRTFRENGLNVTRT 367

Query: 372 DVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESC--TKSPSPESSR 431
           +++T    A N+FYVTD++G     ++IE++R++IGL  L V +  +    K    E  +
Sbjct: 368 EISTSSDMATNIFYVTDANGDEPDFKLIESVREKIGLECLRVKEMPTMYHKKGDGEEQQQ 427

Query: 432 -----FSLGNLFRSRSGKFLYNLGLINSCS 451
                 SLG+L      + L+N GLI SCS
Sbjct: 428 TKAVLVSLGSLV----WRNLFNFGLIKSCS 441

BLAST of CmoCh08G000060 vs. ExPASy TrEMBL
Match: A0A6J1H6F4 (ACT domain-containing protein ACR4-like OS=Cucurbita moschata OX=3662 GN=LOC111460884 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 6.0e-258
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120
           NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA
Sbjct: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120

Query: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180
           AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD
Sbjct: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180

Query: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240
           NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST
Sbjct: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240

Query: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300
           SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ
Sbjct: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300

Query: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360
           EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN
Sbjct: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360

Query: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420
           GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS
Sbjct: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420

Query: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PESSRFSLGNLFRSRSGKFLYNLGLINSCS
Sbjct: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 450

BLAST of CmoCh08G000060 vs. ExPASy TrEMBL
Match: A0A6J1KZ29 (ACT domain-containing protein ACR4-like OS=Cucurbita maxima OX=3661 GN=LOC111497666 PE=4 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 1.9e-251
Identity = 440/450 (97.78%), Postives = 443/450 (98.44%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWS SHPLDDEFEKLVNRMNPPRVT+DNDSC NATLIKVHSANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSPSHPLDDEFEKLVNRMNPPRVTVDNDSCPNATLIKVHSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120
           N TIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA
Sbjct: 61  NLTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120

Query: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180
           AEKHTTIELTGRDRPGLLSEVFAV+ADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD
Sbjct: 121 AEKHTTIELTGRDRPGLLSEVFAVIADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180

Query: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240
           N DRL KIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST
Sbjct: 181 NPDRLAKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240

Query: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300
           SDHRKPLVIVENCTDKGYTVVNLRS DCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ
Sbjct: 241 SDHRKPLVIVENCTDKGYTVVNLRSLDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300

Query: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360
           EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCS+DRAGLLSDVTRIFREN
Sbjct: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSDDRAGLLSDVTRIFREN 360

Query: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420
           GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS
Sbjct: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420

Query: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PESSRFSLGNLFRSRSGKFLYNLGLINS S
Sbjct: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSSS 450

BLAST of CmoCh08G000060 vs. ExPASy TrEMBL
Match: A0A0A0KC82 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G093100 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.4e-227
Identity = 404/451 (89.58%), Postives = 423/451 (93.79%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWSLS PLDDEFEKLVNRMNPPRVT+DNDS   ATLIKV SANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSLSLPLDDEFEKLVNRMNPPRVTVDNDSSRKATLIKVDSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSL-RSVGVQ 120
           N  IRRAYISSDGEWFMDVFHVTD RGNKLSEN VAERIQQSLGPR  SFRSL RSVGVQ
Sbjct: 61  NLIIRRAYISSDGEWFMDVFHVTDQRGNKLSENDVAERIQQSLGPRGRSFRSLRRSVGVQ 120

Query: 121 AAEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPL 180
           AAE+HTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATG P+
Sbjct: 121 AAEEHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGFPI 180

Query: 181 DNTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGS 240
           D+ DRLGKIK+LLL++LKG+RDKRSANTAVSV STHKERRLHQMMY DRDYD+DDLDCGS
Sbjct: 181 DDPDRLGKIKQLLLFVLKGDRDKRSANTAVSVGSTHKERRLHQMMYADRDYDQDDLDCGS 240

Query: 241 TSDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEAT 300
           TS+ RKPLV VE+C DKGYTVVNLRSPD PKLLFDTVCTLTDMQYVVYHATVIAEGPEAT
Sbjct: 241 TSERRKPLVTVESCADKGYTVVNLRSPDRPKLLFDTVCTLTDMQYVVYHATVIAEGPEAT 300

Query: 301 QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRE 360
           QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRR SEGIRLELCS+DRAGLLSDVTRIFRE
Sbjct: 301 QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRTSEGIRLELCSDDRAGLLSDVTRIFRE 360

Query: 361 NGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSP 420
           NGLSVTRA+VTTRG+QAVNVFYVTD+SG+PVK +MIEA+RKEIGLT+L V DDE C KSP
Sbjct: 361 NGLSVTRAEVTTRGTQAVNVFYVTDASGNPVKSEMIEAVRKEIGLTVLCVKDDEFCMKSP 420

Query: 421 SPESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           SPESSRFSLGNLFRSRS KFLYNLGLI SCS
Sbjct: 421 SPESSRFSLGNLFRSRSEKFLYNLGLIKSCS 451

BLAST of CmoCh08G000060 vs. ExPASy TrEMBL
Match: A0A5D3DLZ6 (ACT domain-containing protein ACR4-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002160 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.5e-226
Identity = 404/451 (89.58%), Postives = 421/451 (93.35%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWSLS PLDDEFEKLVNRMNPPRVTIDNDS   ATLIKV SANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSLSLPLDDEFEKLVNRMNPPRVTIDNDSSRKATLIKVDSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSL-RSVGVQ 120
           N  IRRAYISSDGEWFMDVFHVTD  GNKLSEN VAERIQQSLGPRA SFRSL RSVGVQ
Sbjct: 61  NLIIRRAYISSDGEWFMDVFHVTDQHGNKLSENDVAERIQQSLGPRARSFRSLRRSVGVQ 120

Query: 121 AAEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPL 180
           AAE+HTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATG  +
Sbjct: 121 AAEEHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGFSI 180

Query: 181 DNTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGS 240
            + DRLGKIK+LLL++LKG+RDKRSANTAVSV STHKERRLHQMMY DRDYD+DDLDCGS
Sbjct: 181 SDPDRLGKIKQLLLFVLKGDRDKRSANTAVSVGSTHKERRLHQMMYADRDYDQDDLDCGS 240

Query: 241 TSDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEAT 300
           TS+ RKPLV VENC DKGYTVVNLRSPD PKLLFDTVCTLTDMQYVVYHATVIAEGPEA+
Sbjct: 241 TSERRKPLVTVENCADKGYTVVNLRSPDRPKLLFDTVCTLTDMQYVVYHATVIAEGPEAS 300

Query: 301 QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRE 360
           QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRR SEGIRLELCSEDRAGLLSDVTRIFRE
Sbjct: 301 QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRTSEGIRLELCSEDRAGLLSDVTRIFRE 360

Query: 361 NGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSP 420
           NGLSVTRA+VTTRG+QAVNVFYVTD+SG+PVK +MIEA+RKEIGLT+L V DDE C KSP
Sbjct: 361 NGLSVTRAEVTTRGTQAVNVFYVTDASGNPVKSEMIEAVRKEIGLTVLCVKDDEFCMKSP 420

Query: 421 SPESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           SPESSRFSLGNLFRSRS KFLYNLGLI SCS
Sbjct: 421 SPESSRFSLGNLFRSRSEKFLYNLGLIKSCS 451

BLAST of CmoCh08G000060 vs. ExPASy TrEMBL
Match: A0A1S3CB76 (ACT domain-containing protein ACR4-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498865 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.5e-226
Identity = 404/451 (89.58%), Postives = 421/451 (93.35%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWSLS PLDDEFEKLVNRMNPPRVTIDNDS   ATLIKV SANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSLSLPLDDEFEKLVNRMNPPRVTIDNDSSRKATLIKVDSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSL-RSVGVQ 120
           N  IRRAYISSDGEWFMDVFHVTD  GNKLSEN VAERIQQSLGPRA SFRSL RSVGVQ
Sbjct: 61  NLIIRRAYISSDGEWFMDVFHVTDQHGNKLSENDVAERIQQSLGPRARSFRSLRRSVGVQ 120

Query: 121 AAEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPL 180
           AAE+HTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATG  +
Sbjct: 121 AAEEHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGFSI 180

Query: 181 DNTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGS 240
            + DRLGKIK+LLL++LKG+RDKRSANTAVSV STHKERRLHQMMY DRDYD+DDLDCGS
Sbjct: 181 SDPDRLGKIKQLLLFVLKGDRDKRSANTAVSVGSTHKERRLHQMMYADRDYDQDDLDCGS 240

Query: 241 TSDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEAT 300
           TS+ RKPLV VENC DKGYTVVNLRSPD PKLLFDTVCTLTDMQYVVYHATVIAEGPEA+
Sbjct: 241 TSERRKPLVTVENCADKGYTVVNLRSPDRPKLLFDTVCTLTDMQYVVYHATVIAEGPEAS 300

Query: 301 QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRE 360
           QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRR SEGIRLELCSEDRAGLLSDVTRIFRE
Sbjct: 301 QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRTSEGIRLELCSEDRAGLLSDVTRIFRE 360

Query: 361 NGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSP 420
           NGLSVTRA+VTTRG+QAVNVFYVTD+SG+PVK +MIEA+RKEIGLT+L V DDE C KSP
Sbjct: 361 NGLSVTRAEVTTRGTQAVNVFYVTDASGNPVKSEMIEAVRKEIGLTVLCVKDDEFCMKSP 420

Query: 421 SPESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           SPESSRFSLGNLFRSRS KFLYNLGLI SCS
Sbjct: 421 SPESSRFSLGNLFRSRSEKFLYNLGLIKSCS 451

BLAST of CmoCh08G000060 vs. NCBI nr
Match: XP_022959996.1 (ACT domain-containing protein ACR4-like [Cucurbita moschata])

HSP 1 Score: 899.4 bits (2323), Expect = 1.2e-257
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120
           NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA
Sbjct: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120

Query: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180
           AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD
Sbjct: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180

Query: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240
           NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST
Sbjct: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240

Query: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300
           SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ
Sbjct: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300

Query: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360
           EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN
Sbjct: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360

Query: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420
           GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS
Sbjct: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420

Query: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PESSRFSLGNLFRSRSGKFLYNLGLINSCS
Sbjct: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 450

BLAST of CmoCh08G000060 vs. NCBI nr
Match: KAG7020287.1 (ACT domain-containing protein ACR4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897.1 bits (2317), Expect = 6.1e-257
Identity = 449/450 (99.78%), Postives = 449/450 (99.78%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120
           N TIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA
Sbjct: 61  NLTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120

Query: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180
           AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD
Sbjct: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180

Query: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240
           NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST
Sbjct: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240

Query: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300
           SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ
Sbjct: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300

Query: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360
           EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN
Sbjct: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360

Query: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420
           GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS
Sbjct: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420

Query: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PESSRFSLGNLFRSRSGKFLYNLGLINSCS
Sbjct: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 450

BLAST of CmoCh08G000060 vs. NCBI nr
Match: KAG6592805.1 (ACT domain-containing protein ACR4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 449/451 (99.56%), Postives = 449/451 (99.56%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120
           N TIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA
Sbjct: 61  NLTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120

Query: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180
           AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD
Sbjct: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180

Query: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240
           NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST
Sbjct: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240

Query: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEAT- 300
           SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEAT 
Sbjct: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300

Query: 301 QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRE 360
           QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRE
Sbjct: 301 QEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRE 360

Query: 361 NGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSP 420
           NGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSP
Sbjct: 361 NGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSP 420

Query: 421 SPESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           SPESSRFSLGNLFRSRSGKFLYNLGLINSCS
Sbjct: 421 SPESSRFSLGNLFRSRSGKFLYNLGLINSCS 451

BLAST of CmoCh08G000060 vs. NCBI nr
Match: XP_023513709.1 (ACT domain-containing protein ACR4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 888.6 bits (2295), Expect = 2.2e-254
Identity = 445/450 (98.89%), Postives = 446/450 (99.11%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120
           N TI RAYISSDG WFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA
Sbjct: 61  NLTITRAYISSDGGWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120

Query: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180
           AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITD+ATGLPLD
Sbjct: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDDATGLPLD 180

Query: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240
           N DRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST
Sbjct: 181 NPDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240

Query: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300
           SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ
Sbjct: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300

Query: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360
           EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN
Sbjct: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360

Query: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420
           GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS
Sbjct: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420

Query: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PESSRFSLGNLFRSRSGKFLYNLGLINSCS
Sbjct: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 450

BLAST of CmoCh08G000060 vs. NCBI nr
Match: XP_023004303.1 (ACT domain-containing protein ACR4-like [Cucurbita maxima])

HSP 1 Score: 877.9 bits (2267), Expect = 3.8e-251
Identity = 440/450 (97.78%), Postives = 443/450 (98.44%), Query Frame = 0

Query: 1   MDCWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDL 60
           MDCWS SHPLDDEFEKLVNRMNPPRVT+DNDSC NATLIKVHSANKRGSLLEVVQVLNDL
Sbjct: 1   MDCWSPSHPLDDEFEKLVNRMNPPRVTVDNDSCPNATLIKVHSANKRGSLLEVVQVLNDL 60

Query: 61  NFTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120
           N TIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA
Sbjct: 61  NLTIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQA 120

Query: 121 AEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180
           AEKHTTIELTGRDRPGLLSEVFAV+ADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD
Sbjct: 121 AEKHTTIELTGRDRPGLLSEVFAVIADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLD 180

Query: 181 NTDRLGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240
           N DRL KIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST
Sbjct: 181 NPDRLAKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGST 240

Query: 241 SDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300
           SDHRKPLVIVENCTDKGYTVVNLRS DCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ
Sbjct: 241 SDHRKPLVIVENCTDKGYTVVNLRSLDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQ 300

Query: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFREN 360
           EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCS+DRAGLLSDVTRIFREN
Sbjct: 301 EYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSDDRAGLLSDVTRIFREN 360

Query: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420
           GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS
Sbjct: 361 GLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHVIDDESCTKSPS 420

Query: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PESSRFSLGNLFRSRSGKFLYNLGLINS S
Sbjct: 421 PESSRFSLGNLFRSRSGKFLYNLGLINSSS 450

BLAST of CmoCh08G000060 vs. TAIR 10
Match: AT1G69040.1 (ACT domain repeat 4 )

HSP 1 Score: 496.9 bits (1278), Expect = 1.7e-140
Identity = 261/453 (57.62%), Postives = 331/453 (73.07%), Query Frame = 0

Query: 5   SLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNFTI 64
           S S  +D+E+EKL+ RMNPPRV IDNDSC  AT+I+V SAN+ G LLEVVQ+L DLN TI
Sbjct: 2   SFSQDMDNEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTI 61

Query: 65  RRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQAAEKH 124
            +AYISSDG WFMDVF+VTD  GNK+++  V + IQ+SLGP A    S+RSVGV  +   
Sbjct: 62  TKAYISSDGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDS 121

Query: 125 TTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLDNTDR 184
           T IELTG DRPGLLSE+ AVL  LKC+V+ AE+WTHN+R A+V+ +TD+ TG  + + +R
Sbjct: 122 TVIELTGCDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPER 181

Query: 185 LGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRD--DLDCGSTSD 244
           L +IK LL  +LKG+   R A T VS    H +RRLHQMM+EDRDY+    D D     +
Sbjct: 182 LSRIKNLLRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDDSSIQDE 241

Query: 245 HRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQEY 304
            ++P V V+N  DK Y+VV +R  D PKLLFDTVCTLTDMQYVV+H +V  EG EA QEY
Sbjct: 242 RQRPDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEY 301

Query: 305 YIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRENGL 364
           Y+RH+DGSP+ SEAE+QRVI CLEAAI+RR SEG++LELC+ DR GLLS+VTRIFREN L
Sbjct: 302 YVRHIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSL 361

Query: 365 SVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHV----IDDESCTKS 424
           +VTRA+V T+G +A+N FYV+D+SG  +  + I++IR+ IG TIL V     + +   KS
Sbjct: 362 TVTRAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQKS 421

Query: 425 PSPES-SRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PS ES +RF  G LF+S+S     N GL+ S S
Sbjct: 422 PSHESPTRFLFGGLFKSKS---FVNFGLVRSYS 451

BLAST of CmoCh08G000060 vs. TAIR 10
Match: AT1G69040.2 (ACT domain repeat 4 )

HSP 1 Score: 496.9 bits (1278), Expect = 1.7e-140
Identity = 261/453 (57.62%), Postives = 331/453 (73.07%), Query Frame = 0

Query: 5   SLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNFTI 64
           S S  +D+E+EKL+ RMNPPRV IDNDSC  AT+I+V SAN+ G LLEVVQ+L DLN TI
Sbjct: 6   SFSQDMDNEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTI 65

Query: 65  RRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSLRSVGVQAAEKH 124
            +AYISSDG WFMDVF+VTD  GNK+++  V + IQ+SLGP A    S+RSVGV  +   
Sbjct: 66  TKAYISSDGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDS 125

Query: 125 TTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLDNTDR 184
           T IELTG DRPGLLSE+ AVL  LKC+V+ AE+WTHN+R A+V+ +TD+ TG  + + +R
Sbjct: 126 TVIELTGCDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPER 185

Query: 185 LGKIKRLLLYILKGNRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRD--DLDCGSTSD 244
           L +IK LL  +LKG+   R A T VS    H +RRLHQMM+EDRDY+    D D     +
Sbjct: 186 LSRIKNLLRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDDSSIQDE 245

Query: 245 HRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQEY 304
            ++P V V+N  DK Y+VV +R  D PKLLFDTVCTLTDMQYVV+H +V  EG EA QEY
Sbjct: 246 RQRPDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEY 305

Query: 305 YIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRENGL 364
           Y+RH+DGSP+ SEAE+QRVI CLEAAI+RR SEG++LELC+ DR GLLS+VTRIFREN L
Sbjct: 306 YVRHIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSL 365

Query: 365 SVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILHV----IDDESCTKS 424
           +VTRA+V T+G +A+N FYV+D+SG  +  + I++IR+ IG TIL V     + +   KS
Sbjct: 366 TVTRAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQKS 425

Query: 425 PSPES-SRFSLGNLFRSRSGKFLYNLGLINSCS 451
           PS ES +RF  G LF+S+S     N GL+ S S
Sbjct: 426 PSHESPTRFLFGGLFKSKS---FVNFGLVRSYS 455

BLAST of CmoCh08G000060 vs. TAIR 10
Match: AT2G03730.1 (ACT domain repeat 5 )

HSP 1 Score: 453.0 bits (1164), Expect = 2.8e-127
Identity = 256/461 (55.53%), Postives = 323/461 (70.07%), Query Frame = 0

Query: 3   CWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNF 62
           C S S+ +DDE  K + R+NPPRV IDN+ C + T+IKV SANK G LLEVVQVL +LN 
Sbjct: 4   CLSYSYNMDDEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNL 63

Query: 63  TIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALS--FRSLRS-VGVQ 122
           TI++AYISSDG WFMDVF+VTD  GNK+++  V E I++SLGP   S    S+RS +GV+
Sbjct: 64  TIKKAYISSDGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVK 123

Query: 123 AAEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPL 182
            +  +T +ELTG DRPGLLSE+ AVL DL+CNVV AE+WTH ++ A+V+ +TDE T   +
Sbjct: 124 QSVDYTVVELTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAI 183

Query: 183 DNTDRLGKIKRLLLYILKG---NRDKRSANTAVS--VCSTHKERRLHQMMYEDRDYDR-- 242
            + +RL KI++LL Y+L G    R  R   T VS  +  TH +R+LHQ+M+ DRDYD   
Sbjct: 184 TDPERLSKIRKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDEWE 243

Query: 243 ---DDLD-CGSTSDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYH 302
              DD D CG       P V V N  D  Y++V ++  D PKLLFDTV TLTDM YVV H
Sbjct: 244 NNVDDEDKCGRVI----PDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSH 303

Query: 303 ATVIAEGPEATQEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAG 362
           A++ AEGP+A QEYYIRH DGSP+ SEAERQRVI CL+AAI+RR SEG++LELC+ DR G
Sbjct: 304 ASIDAEGPQAYQEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVG 363

Query: 363 LLSDVTRIFRENGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILH 422
           LLSDVTRIFREN L+VTRA+V T+G +A+N FYV D+SG  V  + IE+IR+ IG TIL 
Sbjct: 364 LLSDVTRIFRENSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQ 423

Query: 423 VIDDESCTKSPSPESSRFS-LGNLFRSRSGKFLYNLGLINS 449
           V    +  K PSP+ S    L  +F+SRS     N GLI S
Sbjct: 424 VKGGNTDAK-PSPQDSPTGFLFGVFKSRS---FVNFGLIRS 456

BLAST of CmoCh08G000060 vs. TAIR 10
Match: AT2G03730.2 (ACT domain repeat 5 )

HSP 1 Score: 453.0 bits (1164), Expect = 2.8e-127
Identity = 256/461 (55.53%), Postives = 323/461 (70.07%), Query Frame = 0

Query: 3   CWSLSHPLDDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNF 62
           C S S+ +DDE  K + R+NPPRV IDN+ C + T+IKV SANK G LLEVVQVL +LN 
Sbjct: 4   CLSYSYNMDDEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNL 63

Query: 63  TIRRAYISSDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALS--FRSLRS-VGVQ 122
           TI++AYISSDG WFMDVF+VTD  GNK+++  V E I++SLGP   S    S+RS +GV+
Sbjct: 64  TIKKAYISSDGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVK 123

Query: 123 AAEKHTTIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPL 182
            +  +T +ELTG DRPGLLSE+ AVL DL+CNVV AE+WTH ++ A+V+ +TDE T   +
Sbjct: 124 QSVDYTVVELTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAI 183

Query: 183 DNTDRLGKIKRLLLYILKG---NRDKRSANTAVS--VCSTHKERRLHQMMYEDRDYDR-- 242
            + +RL KI++LL Y+L G    R  R   T VS  +  TH +R+LHQ+M+ DRDYD   
Sbjct: 184 TDPERLSKIRKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDEWE 243

Query: 243 ---DDLD-CGSTSDHRKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYH 302
              DD D CG       P V V N  D  Y++V ++  D PKLLFDTV TLTDM YVV H
Sbjct: 244 NNVDDEDKCGRVI----PDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSH 303

Query: 303 ATVIAEGPEATQEYYIRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAG 362
           A++ AEGP+A QEYYIRH DGSP+ SEAERQRVI CL+AAI+RR SEG++LELC+ DR G
Sbjct: 304 ASIDAEGPQAYQEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVG 363

Query: 363 LLSDVTRIFRENGLSVTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTILH 422
           LLSDVTRIFREN L+VTRA+V T+G +A+N FYV D+SG  V  + IE+IR+ IG TIL 
Sbjct: 364 LLSDVTRIFRENSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQ 423

Query: 423 VIDDESCTKSPSPESSRFS-LGNLFRSRSGKFLYNLGLINS 449
           V    +  K PSP+ S    L  +F+SRS     N GLI S
Sbjct: 424 VKGGNTDAK-PSPQDSPTGFLFGVFKSRS---FVNFGLIRS 456

BLAST of CmoCh08G000060 vs. TAIR 10
Match: AT1G76990.1 (ACT domain repeat 3 )

HSP 1 Score: 438.0 bits (1125), Expect = 9.5e-123
Identity = 223/404 (55.20%), Postives = 295/404 (73.02%), Query Frame = 0

Query: 11  DDEFEKLVNRMNPPRVTIDNDSCSNATLIKVHSANKRGSLLEVVQVLNDLNFTIRRAYIS 70
           D E+E L +R+NPP V+IDN SC   TL+KV S NK G LLEVVQVL DL+ TI +AYIS
Sbjct: 10  DPEYENLSSRINPPSVSIDNTSCKECTLVKVDSMNKPGILLEVVQVLTDLDLTITKAYIS 69

Query: 71  SDGEWFMDVFHVTDHRGNKLSENGVAERIQQSLGPRALSFRSL-----RSVGVQAAEKHT 130
           SDG WFMDVFHVTD +GNK++++   + I++ LGP+  +  S      + VGV +   HT
Sbjct: 70  SDGGWFMDVFHVTDQQGNKVTDSKTIDYIEKVLGPKGHASASQNTWPGKRVGVHSLGDHT 129

Query: 131 TIELTGRDRPGLLSEVFAVLADLKCNVVAAEVWTHNSRMASVVYITDEATGLPLDNTDRL 190
           +IE+  RDRPGLLSEV AVLADL  NVVAAE WTHN R+A V+Y+ D AT   +D+ +RL
Sbjct: 130 SIEIIARDRPGLLSEVSAVLADLNINVVAAEAWTHNRRIACVLYVNDNATSRAVDDPERL 189

Query: 191 GKIKRLLLYILKG--NRDKRSANTAVSVCSTHKERRLHQMMYEDRDYDRDDLDCGSTSDH 250
             ++  L  +L+G   +D++ A T++S+ STH +RRLHQM + DRDY+       S S  
Sbjct: 190 SSMEEQLNNVLRGCEEQDEKFARTSLSIGSTHVDRRLHQMFFADRDYEAVTKLDDSASCG 249

Query: 251 RKPLVIVENCTDKGYTVVNLRSPDCPKLLFDTVCTLTDMQYVVYHATVIAEGPEATQEYY 310
            +P + VE+C +KGY+V+N+   D PKL+FD VCTLTDMQY+V+HAT+ + G  A+QEY+
Sbjct: 250 FEPKITVEHCEEKGYSVINVSCEDRPKLMFDIVCTLTDMQYIVFHATISSSGSHASQEYF 309

Query: 311 IRHMDGSPISSEAERQRVIHCLEAAIRRRNSEGIRLELCSEDRAGLLSDVTRIFRENGLS 370
           IRH DG  + +E E++RV+ CLEAAI RR SEG  LELC++DR GLLS+VTRI RE+GLS
Sbjct: 310 IRHKDGCTLDTEGEKERVVKCLEAAIHRRVSEGWSLELCAKDRVGLLSEVTRILREHGLS 369

Query: 371 VTRADVTTRGSQAVNVFYVTDSSGSPVKIQMIEAIRKEIGLTIL 408
           V+RA VTT G QAVNVFYV D+SG+PV ++ IEA+R EIG +++
Sbjct: 370 VSRAGVTTVGEQAVNVFYVKDASGNPVDVKTIEALRGEIGHSMM 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LJW32.4e-13957.62ACT domain-containing protein ACR4 OS=Arabidopsis thaliana OX=3702 GN=ACR4 PE=2 ... [more]
Q9ZPQ84.0e-12655.53ACT domain-containing protein ACR5 OS=Arabidopsis thaliana OX=3702 GN=ACR5 PE=2 ... [more]
O492851.3e-12155.20ACT domain-containing protein ACR3 OS=Arabidopsis thaliana OX=3702 GN=ACR3 PE=2 ... [more]
Q9SGA08.1e-11953.10ACT domain-containing protein ACR6 OS=Arabidopsis thaliana OX=3702 GN=ACR6 PE=1 ... [more]
Q9LNA55.1e-11350.89ACT domain-containing protein ACR8 OS=Arabidopsis thaliana OX=3702 GN=ACR8 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1H6F46.0e-258100.00ACT domain-containing protein ACR4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KZ291.9e-25197.78ACT domain-containing protein ACR4-like OS=Cucurbita maxima OX=3661 GN=LOC111497... [more]
A0A0A0KC822.4e-22789.58Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G093100 PE=4 SV=1[more]
A0A5D3DLZ63.5e-22689.58ACT domain-containing protein ACR4-like isoform X2 OS=Cucumis melo var. makuwa O... [more]
A0A1S3CB763.5e-22689.58ACT domain-containing protein ACR4-like isoform X2 OS=Cucumis melo OX=3656 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_022959996.11.2e-257100.00ACT domain-containing protein ACR4-like [Cucurbita moschata][more]
KAG7020287.16.1e-25799.78ACT domain-containing protein ACR4 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6592805.11.5e-25599.56ACT domain-containing protein ACR4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023513709.12.2e-25498.89ACT domain-containing protein ACR4-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023004303.13.8e-25197.78ACT domain-containing protein ACR4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G69040.11.7e-14057.62ACT domain repeat 4 [more]
AT1G69040.21.7e-14057.62ACT domain repeat 4 [more]
AT2G03730.12.8e-12755.53ACT domain repeat 5 [more]
AT2G03730.22.8e-12755.53ACT domain repeat 5 [more]
AT1G76990.19.5e-12355.20ACT domain repeat 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002912ACT domainPFAMPF01842ACTcoord: 339..400
e-value: 3.9E-8
score: 32.9
coord: 38..87
e-value: 1.1E-6
score: 28.3
coord: 125..173
e-value: 3.8E-10
score: 39.4
IPR002912ACT domainPROSITEPS51671ACTcoord: 126..203
score: 13.713675
IPR002912ACT domainPROSITEPS51671ACTcoord: 38..118
score: 9.759089
IPR002912ACT domainPROSITEPS51671ACTcoord: 338..417
score: 13.132343
NoneNo IPR availableGENE3D3.30.70.260coord: 120..191
e-value: 1.4E-9
score: 39.6
coord: 335..409
e-value: 2.6E-6
score: 29.1
NoneNo IPR availablePANTHERPTHR31096:SF15OS08G0515400 PROTEINcoord: 2..447
NoneNo IPR availableCDDcd04897ACT_ACR_3coord: 258..332
e-value: 3.67027E-36
score: 125.996
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 33..103
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 122..178
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 334..402
IPR040217ACT domain-containing protein ACR1-12PANTHERPTHR31096ACT DOMAIN-CONTAINING PROTEIN ACR4-RELATEDcoord: 2..447

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G000060.1CmoCh08G000060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016779 nucleotidyltransferase activity