CmoCh07G011690 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh07G011690
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionNADH--cytochrome b5 reductase 1-like
LocationCmo_Chr07: 6270399 .. 6274384 (-)
RNA-Seq ExpressionCmoCh07G011690
SyntenyCmoCh07G011690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCACCTAACTCGTTCGTTTCATTTTTCTCCGGTGGCTGCTGCTGCATTCGGTGAAAGTGGGCTCTGGAGCTGTGGAACGGAAAAAGTTAGGTGAATCACAACTCCATTTGATTTTTCTCAAGGTTTTTGTCTGTGAAAAATCGTGTAATGGACTTATTACAGTCTTTGGATCCTCAAATCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCTCTTGCCTTTGGTGCTCTCTATTTGTTATCCTCGTCCAAGAAAACCAAATCCGGAGGTTAGCTGCTCTTGAATCTCAGTTCATTTATTATTATTATTATTATTATTTAATTTCCAGTGTTTGGATTTCCTTGTTTAGTGTTTTGGATTCATGATTTGCGGTTGAAATCTACTTTACCTGAATGTTGTAAGCAGTTTCTAGGATCTACTGTGTTTTTATTCGTGCCATTTTGATGAAATTTTGAGAGTGCAGTTTGAAGTTCTTGTTACTTTGTGGATATGTGGACGTCTGGAGTCTGAATGTTACTATAGTTACTCTGTAATGGACATTTCTGTTCAAATTAAGCGTATGATCTGTTTGAATTGGTACTAGTGAAGGAAATTTCAGGGAACACTTCCATAACCATGCGAGTTCCTACTCCCTTTGATAATATGAAGTACTAATGCAGTAATATGTGGACAAATGGATTTTTCACTCAACATTTTCAGTGGATTCTTGCGCATATGGAAGGTTTCATTGTGATGATACATTAGAACATCGTCTGTCATTGGTGTTTTATTCGATTCATTTTCGTACGTTTTTTTCCTTACAATTGGTTCGTGCATTCAGGTTGCTTGGATCCAGAGAATTTCAAGGAGTTTAAACTCATCAAGCGTGCGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCAAGTTCTACTTCAGTTTTGGGCCTTCCCATTGGACAACATATAAGTTGCAGGTCTAGTAGTTTATCCTTATTCATTTGTCATAGGATTAGTTCGAATCTCGATACTATACTCTCTCGTGTTGACTCTGTAGTCCTTAAAGTACTTGTTTATTTATTTGTTATTGTTTGGGAGTAATCTCTAAGAACGTTGTTTAGGTGGAGTATCATGTGTTTCTTTTAGCTGCTTAATTGTGTTCAAAACACTTTTCTAATAATTTGCATCATCTGAAACAAGCTAACAGAAATGTTTTTCTTAATCTCCCTTCGGTTTGAATGGGGATGAATGCCTTATTGCCCTATTCACCTGGTGTTGTTTTTTAAGATGATTCTGTTTCATATGACAAAAAGGATTTATTTCTCCTGAGAAACTATAATTCATCCATCCTACAGGGGCAAGGATGGTCAAGGTGAAGATGTTATCAAACCTTATACACCTATTACTTTGGATTCTGATGTCGGATATTTTGAATTAGTCATAAAGGTACGTATCTTTGTGTTTTTTTTTTTTTTTTTAAATGTTTCACATGCATGTTATTCTTAATCCTAACTCTTTTGTGTTTAATCAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAGATGCGTGTCGGTGATTTTCTGTCTGTGAAAGGGCCCAAGGTATGTGTCGATCAATTAATGGCAGCTATTTAAGTTATCATTTCTTATGCTTTTAGGTATCTATGTTTTTTGGGGTTCTTAACATTTTATCCATTGACAAATGACTGGCATTTTTCTTTGTCCAAACTTGTCTCATACACGTTCTTCACATATTTGATCTCTGATGTTATTTTCTGTATGTCTAAATCAATTCATGTTTTCGTTTTTTATAATTCTCTTTTGAATTAATACTGTGACTCAATTTATGTTGGAAGCCTTCTCTTGATCAGTGATTTTGAAACAATTTTGACAGTAAACTAGAAAACGAAGAAAGATTTTAGTAAATAGGTGTAATTTTATTAAACAGAGAACAGTATTAAAACTCGTGGCCTTCAATGTTCAAGTTCAAGTTGGTATGGAAATTAATCTCCTGTATCTACTGCCCTGGTTTATGTTATCTATCTGTCTGTCTAGCATTCAGCTTTCTTAGCGTTGGCCATGGACTTTTCCTCCCACGCTCTATTTCGAGAATTTAATGGAAACATTTTATTTGTCGCAGAGAGAAACAAATAACTGCATAGTACCTTTGAAAACGCTGAGAATATTCAAAATTTACAATGTAAATATTACTGCAGGGGCGATTCAGGTATCAACCAGGCGAGGTCAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACTCCTATGTATCAAGTACATATTTCCTTTACTTTTGGCTTGCTCATTTTTGTTCCTCAACATACTATCATTTGGATTGCTTTGATCGTTGTATGGATATTCATGCTTTCGTTGCTCTCCTTAGGTTGCTCGAGCCATATTAGAAAATCCAAATGACAAGACGAAGGTACATCTCATTTATGCCAACGTCACCTACGAGGACATTCTTTTGAAGGTAATTTTGCCATATCTTCTTCAATGGAGTTCCCTTTTGTCTTCAATTTGACCAAAATGTGGATACCTGTACATCTTTTTATTAACTTTTACTTTCATGGAGAGAGATAAGCCATGCCAACCAGTGCGACCGAGGCTTATTTTCCACACTGTCTCAGTGATATTGTAATGAATCTACTCCTTTCTCAGCGCGCTATGAATTTCAACGGCATAGTGATTTGATTGGAATGTTTTCAGTACATCTAATGTTCGAACTCCTTAGTTTAACACTTGTGTGGAGTTCTTTTGCCAACATGAACATAGCGAACATAGCTTAACTAGTTAAGTCATATAATCCTCTGTCCTTGTCATAGCTCGACTGTCCTCAACTAAAAAGGCCAGTTGTTCATCATTTTATATAGATAAGAAAAACGTGTAGGATCTTAATATTTTTGTAGCTGCCTCTTGGGCTTCACTTTTGCTTTTATAACAATAATCCTGTTTCTATGATACCTCTTTCTCCAATTTCAGAACTGTTTCTTACCAAAAGTAACTTGTGTGGTTGTAGGAGGAGTTGGATCTTCTTGCCTCAAGGTACCCAGATATCTTCAAGCTCTATTATGTTTTAAACCAGGTCAGTCTTCTCCTTCAGCCACAACGCCAAATTTATACTTATCTCTACCATATCTTTTGATCAACCATTTGTATATCCATTGCTTACCCAGCCTCCTGAATCGTGGGACGGCGGTGTTGGATTCGTGACGAAGGAGATGATTCAAACCCATTGCCCTGCTCCTGCTTCTGATGTTCGGGTATGTTTGATTACCAACTCATTAGTTTTAAATGACAAGGATGCTTCAGATTGGTTCTCAAAAACATGTTTGTGAACAATTTCCTTTGTCAAAAGTGCTTTCGCTCGATGTTTCTACGAATAACCTACTTTTTAAAGCTAACGAAGAGTGATCTCGTGTGCCAGAAATTGTTGGATTGGAGTAGGTTCATTAGATTCATTTACGTGTCTATATTTTGATCGAAATTTTAAACCACTAAATCATTACAAAGTGTTGTATGAGCTTTACCTCATATGTAACCCTGTTTTATAAGCAGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCTATGGCTGCTCATCTTGAAGCTCTTGGATACGCACCAGAGATGCTGTTCATGTTCTGATTCTTCCTCAAAGTTCTTCACTAGAACTAAGCATCATTTTTCTTTTCCTCCCAAGTTTTGAAAATTTGAATAATGTCTCAATTTAGAGCTAAAGATTTATGTACTTAGGAGGCTGTTTTCTTAGCTCTTTTTCATTTTGCAATGCCGTGGAATAATTGATGCCATCATTTAGCAGAGTCCACCCTAGTTTTTTTATTTGGCTGAAATTGTCTTTTCAAGAGCAACTTTGAAATAAGCTTTATGAGCCTATAATAGGCGGGTGGCAACTACGAGACTGAGATAGTTTATATTTGAAATGAAATTTGATTTGATTGTCATATCAA

mRNA sequence

CCACCTAACTCGTTCGTTTCATTTTTCTCCGGTGGCTGCTGCTGCATTCGGTGAAAGTGGGCTCTGGAGCTGTGGAACGGAAAAAGTTAGGTGAATCACAACTCCATTTGATTTTTCTCAAGGTTTTTGTCTGTGAAAAATCGTGTAATGGACTTATTACAGTCTTTGGATCCTCAAATCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCTCTTGCCTTTGGTGCTCTCTATTTGTTATCCTCGTCCAAGAAAACCAAATCCGGAGGTTGCTTGGATCCAGAGAATTTCAAGGAGTTTAAACTCATCAAGCGTGCGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCAAGTTCTACTTCAGTTTTGGGCCTTCCCATTGGACAACATATAAGTTGCAGGGGCAAGGATGGTCAAGGTGAAGATGTTATCAAACCTTATACACCTATTACTTTGGATTCTGATGTCGGATATTTTGAATTAGTCATAAAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAGATGCGTGTCGGTGATTTTCTGTCTGTGAAAGGGCCCAAGGGGCGATTCAGGTATCAACCAGGCGAGGTCAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACTCCTATGTATCAAGTTGCTCGAGCCATATTAGAAAATCCAAATGACAAGACGAAGGTACATCTCATTTATGCCAACGTCACCTACGAGGACATTCTTTTGAAGGAGGAGTTGGATCTTCTTGCCTCAAGGTACCCAGATATCTTCAAGCTCTATTATGTTTTAAACCAGCCTCCTGAATCGTGGGACGGCGGTGTTGGATTCGTGACGAAGGAGATGATTCAAACCCATTGCCCTGCTCCTGCTTCTGATGTTCGGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCTATGGCTGCTCATCTTGAAGCTCTTGGATACGCACCAGAGATGCTGTTCATGTTCTGATTCTTCCTCAAAGTTCTTCACTAGAACTAAGCATCATTTTTCTTTTCCTCCCAAGTTTTGAAAATTTGAATAATGTCTCAATTTAGAGCTAAAGATTTATGTACTTAGGAGGCTGTTTTCTTAGCTCTTTTTCATTTTGCAATGCCGTGGAATAATTGATGCCATCATTTAGCAGAGTCCACCCTAGTTTTTTTATTTGGCTGAAATTGTCTTTTCAAGAGCAACTTTGAAATAAGCTTTATGAGCCTATAATAGGCGGGTGGCAACTACGAGACTGAGATAGTTTATATTTGAAATGAAATTTGATTTGATTGTCATATCAA

Coding sequence (CDS)

ATGGACTTATTACAGTCTTTGGATCCTCAAATCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCTCTTGCCTTTGGTGCTCTCTATTTGTTATCCTCGTCCAAGAAAACCAAATCCGGAGGTTGCTTGGATCCAGAGAATTTCAAGGAGTTTAAACTCATCAAGCGTGCGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCAAGTTCTACTTCAGTTTTGGGCCTTCCCATTGGACAACATATAAGTTGCAGGGGCAAGGATGGTCAAGGTGAAGATGTTATCAAACCTTATACACCTATTACTTTGGATTCTGATGTCGGATATTTTGAATTAGTCATAAAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAGATGCGTGTCGGTGATTTTCTGTCTGTGAAAGGGCCCAAGGGGCGATTCAGGTATCAACCAGGCGAGGTCAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACTCCTATGTATCAAGTTGCTCGAGCCATATTAGAAAATCCAAATGACAAGACGAAGGTACATCTCATTTATGCCAACGTCACCTACGAGGACATTCTTTTGAAGGAGGAGTTGGATCTTCTTGCCTCAAGGTACCCAGATATCTTCAAGCTCTATTATGTTTTAAACCAGCCTCCTGAATCGTGGGACGGCGGTGTTGGATTCGTGACGAAGGAGATGATTCAAACCCATTGCCCTGCTCCTGCTTCTGATGTTCGGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCTATGGCTGCTCATCTTGAAGCTCTTGGATACGCACCAGAGATGCTGTTCATGTTCTGA

Protein sequence

MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSHNVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQTHCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF
Homology
BLAST of CmoCh07G011690 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 1.5e-131
Identity = 227/280 (81.07%), Postives = 253/280 (90.36%), Query Frame = 0

Query: 2   DLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSHN 61
           + L++LD QILLG+ VA +A+  GA Y L+SSKK +   CLDPENFKEFKL+KR QLSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKKRRV--CLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYPQ 121
           VAKF F LP+STSVLGLPIGQHISCRGKDGQGEDVIKPYTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPND 181
           GRMSHHFR+MRVGD L+VKGPKGRF+YQPG+ RAFGMLAGGSGITPM+QVARAILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQT 241
           KTKVHLIYANVTY+DILLKEEL+ L + YP+ FK++YVLNQPPE WDGGVGFV+KEMIQT
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           HCPAPASD++ILRCGPPPMNKAMAA+LEALGY+PEM F F
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

BLAST of CmoCh07G011690 vs. ExPASy Swiss-Prot
Match: Q54NC1 (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 2.9e-74
Identity = 137/278 (49.28%), Postives = 190/278 (68.35%), Query Frame = 0

Query: 17  VAVLALAFGALY---LLSSSKKTKSGG----------CLDPENFKEFKLIKRAQLSHNVA 76
           +  +ALA G  Y   L S S K K GG           LDP+ +++F+L ++  ++HN  
Sbjct: 11  IGSVALAAGVKYVFTLTSGSNKDKKGGEAEKGKQVEKALDPQEYRKFQLKEKFIVNHNTR 70

Query: 77  KFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYPQGR 136
            F F LP+   +LGLPIGQHIS R   G G++V +PYTPI+ D + GYF+L+IK+Y +G 
Sbjct: 71  IFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYTPISSDEERGYFDLLIKVYEKGA 130

Query: 137 MSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPNDKT 196
           MS +   M +GD + VKGPKG+F YQP   ++ GMLAGG+GITPM QV +AIL+NP+DKT
Sbjct: 131 MSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAGGTGITPMLQVIKAILKNPSDKT 190

Query: 197 KVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQTHC 256
           ++ L++ N+T EDILLK+ELD LA ++P  FK+YYVLN PP+ W  GVGFV+KE+I++  
Sbjct: 191 EISLVFGNITEEDILLKKELDELAEKHPQ-FKVYYVLNNPPKGWTQGVGFVSKEIIESRL 250

Query: 257 PAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           P+P+    ++ CGPP MNKAM  HLE +G+    +F F
Sbjct: 251 PSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

BLAST of CmoCh07G011690 vs. ExPASy Swiss-Prot
Match: Q4X0B5 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 271.9 bits (694), Expect = 7.9e-72
Identity = 136/264 (51.52%), Postives = 179/264 (67.80%), Query Frame = 0

Query: 16  AVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSHNVAKFTFTLPSSTSV 75
           AVAV A+  G       +K  K    L+P  F+EF L ++  +SHNV  + F LP  T +
Sbjct: 34  AVAVTAILAGLKLFTGGNKPRK---VLNPTEFQEFVLKEKIDVSHNVCIYRFALPRPTDI 93

Query: 76  LGLPIGQHISCRGK-DGQGEDVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVG 135
           LGLPIGQHIS     +GQ ++V++ YTPI+ D++ GYF+L++K YPQG +S +   +++G
Sbjct: 94  LGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIG 153

Query: 136 DFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILEN-----PNDKTKVHLIY 195
           D + V+GPKG   Y P   R  GM+AGG+GITPM Q+ +A++ N      ND TK+ LI+
Sbjct: 154 DTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIF 213

Query: 196 ANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQTHCPAPASD 255
           ANV  +DILLKEELD+LA+  PD F +YYVLN PP+ W GGVGFVT EMI+ H PAPASD
Sbjct: 214 ANVNPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASD 273

Query: 256 VRILRCGPPPMNKAMAAHLEALGY 274
           V+IL CGPPPM  AM    E+LGY
Sbjct: 274 VKILLCGPPPMISAMKKATESLGY 293

BLAST of CmoCh07G011690 vs. ExPASy Swiss-Prot
Match: Q59P03 (NADH-cytochrome b5 reductase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 7.9e-72
Identity = 132/257 (51.36%), Postives = 174/257 (67.70%), Query Frame = 0

Query: 17  VAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSHNVAKFTFTLPSSTSVL 76
           VA +  AF   Y L  SKK      L P+ F++F LI++ ++SHN A + F LP ST  L
Sbjct: 25  VATIISAFIGYYFLQQSKKHTP--VLKPDEFQKFPLIEKIRVSHNSAIYRFGLPKSTDRL 84

Query: 77  GLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDF 136
           GLPIGQHIS  G    G++V++ YTPI+ D  +G+F+L+IK Y  G +S H     VG+ 
Sbjct: 85  GLPIGQHISI-GATIDGKEVVRSYTPISTDDQLGHFDLLIKTYENGNISRHVAGKNVGEH 144

Query: 137 LSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYED 196
           + ++GPKG F Y P  V++FGM+AGG+GI PMYQ+  AIL+NP DKTK+HL+YANVT  D
Sbjct: 145 IEIRGPKGFFTYTPNMVKSFGMIAGGTGIAPMYQIITAILKNPEDKTKIHLVYANVTESD 204

Query: 197 ILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQTHCPAPASDVRILRCG 256
           ILLKEELD  A+R+PD  K++YVLN+ P +W G VGFVT E+I TH P  ++D  +L CG
Sbjct: 205 ILLKEELDNFAARHPDRLKIHYVLNEAPANWQGSVGFVTPEIIDTHLPKASNDTNLLLCG 264

Query: 257 PPPMNKAMAAHLEALGY 274
           PPPM  AM      LG+
Sbjct: 265 PPPMVSAMKKAAVELGF 278

BLAST of CmoCh07G011690 vs. ExPASy Swiss-Prot
Match: Q4PGW7 (NADH-cytochrome b5 reductase 1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 7.9e-72
Identity = 129/261 (49.43%), Postives = 181/261 (69.35%), Query Frame = 0

Query: 13  LGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSHNVAKFTFTLPSS 72
           L + +A +    G++ +L      K    L+P  +++++L+++ +LS N A + F LP S
Sbjct: 49  LNIVLAFVVGLIGSVVVLLYFDSQKIKPVLNPTQWQQYRLMEKQKLSDNTALYRFKLPRS 108

Query: 73  TSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMR 132
            ++LGLPIGQHIS +   G G+ V++ YTP + D D G+F+LV+K Y QG +S +   M+
Sbjct: 109 NNILGLPIGQHISVQANMG-GKTVVRSYTPTSSDDDHGFFDLVVKSYEQGNVSKYIGSMK 168

Query: 133 VGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANV 192
           +GD LSVKGPKG+ RY PG  R  GM+AGG+G+TP  Q+ RA L+NP DKT++  IYANV
Sbjct: 169 IGDLLSVKGPKGQMRYAPGLSRHIGMIAGGTGLTPCLQIIRAALKNPADKTQIDFIYANV 228

Query: 193 TYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQTHCPAPASDVRI 252
              DILLK+ELD LA ++ D F++ Y LN+ PE W GGVGFVTKE ++ + P PA+D+++
Sbjct: 229 KETDILLKDELDELALKHKDQFRISYFLNEAPEGWKGGVGFVTKEALEKNLPKPANDIKV 288

Query: 253 LRCGPPPMNKAMAAHLEALGY 274
           L CGPPPM KAM  HLEALGY
Sbjct: 289 LMCGPPPMIKAMTGHLEALGY 308

BLAST of CmoCh07G011690 vs. ExPASy TrEMBL
Match: A0A6J1EBT4 (NADH--cytochrome b5 reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111432569 PE=4 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 2.3e-159
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH
Sbjct: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

BLAST of CmoCh07G011690 vs. ExPASy TrEMBL
Match: A0A6J1HPA2 (NADH--cytochrome b5 reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466076 PE=4 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.5e-158
Identity = 279/281 (99.29%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH
Sbjct: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLASRYPD FKLYYVLNQPPESWDGG+GFVTKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDSFKLYYVLNQPPESWDGGIGFVTKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

BLAST of CmoCh07G011690 vs. ExPASy TrEMBL
Match: A0A5A7TLD5 (NADH--cytochrome b5 reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G001310 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 3.7e-149
Identity = 262/281 (93.24%), Postives = 271/281 (96.44%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MD LQSLDPQILLGLAVAVLALA GALY+LSSSKK+KS GCLDPE FKEFKLIKR QLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLP+ TSVLGLPIGQHISCRGKDGQGE+VIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFL+VKGPKGRFRYQPG+VRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLA RYPD FKLYYVLNQPPE WDGGVGFV+KEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASD++ILRCGPPPMNKAMAAHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

BLAST of CmoCh07G011690 vs. ExPASy TrEMBL
Match: A0A1S3C2D1 (NADH--cytochrome b5 reductase 1 OS=Cucumis melo OX=3656 GN=LOC103495874 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 3.7e-149
Identity = 262/281 (93.24%), Postives = 271/281 (96.44%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MD LQSLDPQILLGLAVAVLALA GALY+LSSSKK+KS GCLDPE FKEFKLIKR QLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLP+ TSVLGLPIGQHISCRGKDGQGE+VIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFL+VKGPKGRFRYQPG+VRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLA RYPD FKLYYVLNQPPE WDGGVGFV+KEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASD++ILRCGPPPMNKAMAAHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

BLAST of CmoCh07G011690 vs. ExPASy TrEMBL
Match: A0A0A0L226 (FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G416480 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 8.3e-149
Identity = 261/281 (92.88%), Postives = 271/281 (96.44%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MD LQSLDPQILLG+AVAVLALA GALY+LSSSKK+KS GCLDPE FKEFKLIKR QLSH
Sbjct: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPS TSVLGLPIGQHISC GKDGQGE+VIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFL+VKGPKGRFRYQPG+VRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLA RYPD FKLYYVLNQPPE+WDGGVGFV+KEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASD++ILRCGPPPMNKAMAAHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

BLAST of CmoCh07G011690 vs. NCBI nr
Match: XP_022925286.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita moschata])

HSP 1 Score: 571.2 bits (1471), Expect = 4.8e-159
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH
Sbjct: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

BLAST of CmoCh07G011690 vs. NCBI nr
Match: KAG7027451.1 (NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568.9 bits (1465), Expect = 2.4e-158
Identity = 280/281 (99.64%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH
Sbjct: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLASRYPD FKLYYVLNQPPESWDGGVGFVTKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDSFKLYYVLNQPPESWDGGVGFVTKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

BLAST of CmoCh07G011690 vs. NCBI nr
Match: XP_022966421.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita maxima])

HSP 1 Score: 568.5 bits (1464), Expect = 3.1e-158
Identity = 279/281 (99.29%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH
Sbjct: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLASRYPD FKLYYVLNQPPESWDGG+GFVTKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDSFKLYYVLNQPPESWDGGIGFVTKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

BLAST of CmoCh07G011690 vs. NCBI nr
Match: KAG6595451.1 (ABC transporter F family member 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 565.5 bits (1456), Expect = 2.6e-157
Identity = 278/280 (99.29%), Postives = 279/280 (99.64%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH
Sbjct: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLASRYPD FKLYYVLNQPPESWDGGVGFVTKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDSFKLYYVLNQPPESWDGGVGFVTKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFM 281
           THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLF+
Sbjct: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFI 280

BLAST of CmoCh07G011690 vs. NCBI nr
Match: XP_023517049.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.9 bits (1452), Expect = 7.7e-157
Identity = 277/281 (98.58%), Postives = 279/281 (99.29%), Query Frame = 0

Query: 1   MDLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSH 60
           MDLLQSLDPQILLG AVAVLALAFGALYLLSSSKK+KSGGCLDPENFKEFKLIKRAQLSH
Sbjct: 1   MDLLQSLDPQILLGSAVAVLALAFGALYLLSSSKKSKSGGCLDPENFKEFKLIKRAQLSH 60

Query: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLASRYPD FKLYYVLNQPPESWDGGVGFVTKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLASRYPDSFKLYYVLNQPPESWDGGVGFVTKEMIQ 240

Query: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEM+FMF
Sbjct: 241 THCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMVFMF 281

BLAST of CmoCh07G011690 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 470.3 bits (1209), Expect = 1.1e-132
Identity = 227/280 (81.07%), Postives = 253/280 (90.36%), Query Frame = 0

Query: 2   DLLQSLDPQILLGLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSHN 61
           + L++LD QILLG+ VA +A+  GA Y L+SSKK +   CLDPENFKEFKL+KR QLSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKKRRV--CLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMYPQ 121
           VAKF F LP+STSVLGLPIGQHISCRGKDGQGEDVIKPYTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFRQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPND 181
           GRMSHHFR+MRVGD L+VKGPKGRF+YQPG+ RAFGMLAGGSGITPM+QVARAILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTKVHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQT 241
           KTKVHLIYANVTY+DILLKEEL+ L + YP+ FK++YVLNQPPE WDGGVGFV+KEMIQT
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDVRILRCGPPPMNKAMAAHLEALGYAPEMLFMF 282
           HCPAPASD++ILRCGPPPMNKAMAA+LEALGY+PEM F F
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

BLAST of CmoCh07G011690 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 215.7 bits (548), Expect = 4.8e-56
Identity = 114/286 (39.86%), Postives = 171/286 (59.79%), Query Frame = 0

Query: 14  GLAVAVLALAFGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSHNVAKFTFTLPSST 73
           G +   L      +YL  + ++T     L+P+ + EFKL   A++SHN   F F+   S 
Sbjct: 45  GFSYYYLTSGNNLVYLDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSA 104

Query: 74  SVLGLPIGQHISCR-----GKDGQGEDVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHF 133
             LGL +   +  R       +G+ + VI+PYTPI+     GYF+L+IK+YP G+MS HF
Sbjct: 105 E-LGLHVASCLLTRAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHF 164

Query: 134 RQMRVGDFLSVKGPKGRFRYQPGEVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLI 193
             ++ GD L VKGP  +F+Y P   +  GM+AGGSGITPM QV  AI++NP D T++ L+
Sbjct: 165 ASLKPGDVLEVKGPVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLL 224

Query: 194 YANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQTHCPAPAS 253
           YANV+ +DILLK++LD+L + +P++ K++Y ++ P ++W GGVG+++K+M     P P  
Sbjct: 225 YANVSPDDILLKQKLDVLQANHPNL-KIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTD 284

Query: 254 DVRILRCGPPPMNKAMAAH-------------LEALGYAPEMLFMF 282
           D  IL CGPP M + ++               L+ LGY  EM+F F
Sbjct: 285 DTLILVCGPPGMMEHISGGKAPDWSQGEVKGILKELGYTEEMVFKF 328

BLAST of CmoCh07G011690 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 173.7 bits (439), Expect = 2.1e-43
Identity = 98/271 (36.16%), Postives = 152/271 (56.09%), Query Frame = 0

Query: 24  FGALYLLSSSKKTKSGGCLDPENFKEFKLIKRAQLSHNVAKFTFTLPSSTSVLGLPIGQH 83
           F  L  +  +   ++   ++P      +L+++  +SH+V KF F LP    VLGLP+G+H
Sbjct: 639 FSLLAPIGEATPVRNLALVNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKH 698

Query: 84  ISCRGKDGQGEDVIKPYTPITLDSDVGYFELVIKMY-----PQ----GRMSHHFRQMRVG 143
           I         +  ++ YTP +    VGYFELV+K+Y     P+    G MS +   + +G
Sbjct: 699 IFLCATIND-KLCLRAYTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIG 758

Query: 144 DFLSVKGPKGRFRY----------QPGEVRAFGMLAGGSGITPMYQVARAILENPNDKTK 203
             L +KGP G   Y          +P       MLAGG+GITP+YQ+ +AIL++P D+T+
Sbjct: 759 STLEIKGPLGHVEYLGKGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETE 818

Query: 204 VHLIYANVTYEDILLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQTHCP 263
           +++IYAN T EDILL+EELD  A +YPD  K++YV+    E W    GF+++ +++ H P
Sbjct: 819 MYVIYANRTEEDILLREELDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIP 878

Query: 264 APASDVRI-LRCGPPPMNK-AMAAHLEALGY 274
                  + + CGPPPM + A+  +LE + Y
Sbjct: 879 DGLDGSALAMACGPPPMIQFAVQPNLEKMQY 908

BLAST of CmoCh07G011690 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 170.6 bits (431), Expect = 1.8e-42
Identity = 90/258 (34.88%), Postives = 148/258 (57.36%), Query Frame = 0

Query: 37  KSGGCLDPENFKEFKLIKRAQLSHNVAKFTFTLPSSTSVLGLPIGQHISCRGKDGQGEDV 96
           K+   ++P      +LI++  +SH+V KF F LPS    LGLP+G+H+         +  
Sbjct: 652 KNIALVNPREKIPVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANIND-KLC 711

Query: 97  IKPYTPITLDSDVGYFELVIKMY-----PQ----GRMSHHFRQMRVGDFLSVKGPKGRFR 156
           ++ YTP +    VG+ +LV+K+Y     P+    G MS H   + +G  + +KGP G   
Sbjct: 712 LRAYTPTSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIE 771

Query: 157 Y----------QPGEVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDI 216
           Y          +P   +   MLAGG+GITP+YQ+ ++IL +P D+T+++++YAN T +DI
Sbjct: 772 YKGKGNFLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDI 831

Query: 217 LLKEELDLLASRYPDIFKLYYVLNQPPESWDGGVGFVTKEMIQTHCPAPASDVRI-LRCG 274
           L++EEL+  AS++ +  K++YV+    E W    GF+T+ +++ H P       + L CG
Sbjct: 832 LVREELEGWASKHKERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACG 891

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZNT11.5e-13181.07NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=... [more]
Q54NC12.9e-7449.28NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE... [more]
Q4X0B57.9e-7251.52NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A... [more]
Q59P037.9e-7251.36NADH-cytochrome b5 reductase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-287... [more]
Q4PGW77.9e-7249.43NADH-cytochrome b5 reductase 1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=23... [more]
Match NameE-valueIdentityDescription
A0A6J1EBT42.3e-159100.00NADH--cytochrome b5 reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114325... [more]
A0A6J1HPA21.5e-15899.29NADH--cytochrome b5 reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466076... [more]
A0A5A7TLD53.7e-14993.24NADH--cytochrome b5 reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C2D13.7e-14993.24NADH--cytochrome b5 reductase 1 OS=Cucumis melo OX=3656 GN=LOC103495874 PE=4 SV=... [more]
A0A0A0L2268.3e-14992.88FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_... [more]
Match NameE-valueIdentityDescription
XP_022925286.14.8e-159100.00NADH--cytochrome b5 reductase 1-like [Cucurbita moschata][more]
KAG7027451.12.4e-15899.64NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022966421.13.1e-15899.29NADH--cytochrome b5 reductase 1-like [Cucurbita maxima][more]
KAG6595451.12.6e-15799.29ABC transporter F family member 4, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023517049.17.7e-15798.58NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G17770.11.1e-13281.07NADH:cytochrome B5 reductase 1 [more]
AT5G20080.14.8e-5639.86FAD/NAD(P)-binding oxidoreductase [more]
AT1G37130.12.1e-4336.16nitrate reductase 2 [more]
AT1G77760.11.8e-4234.88nitrate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001834NADH:cytochrome b5 reductase-likePRINTSPR00406CYTB5RDTASEcoord: 157..176
score: 66.67
coord: 76..87
score: 57.47
coord: 98..105
score: 41.41
coord: 251..259
score: 59.26
coord: 195..206
score: 64.06
coord: 134..148
score: 49.44
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 158..264
e-value: 6.7E-30
score: 103.9
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 140..281
e-value: 1.9E-44
score: 152.9
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 137..278
IPR008333Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domainPFAMPF00970FAD_binding_6coord: 50..148
e-value: 7.3E-32
score: 109.7
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 40..139
e-value: 1.4E-34
score: 120.7
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 2..281
NoneNo IPR availablePANTHERPTHR19370:SF203NADH-CYTOCHROME B5 REDUCTASEcoord: 2..281
NoneNo IPR availableCDDcd06183cyt_b5_reduct_likecoord: 50..281
e-value: 9.86491E-119
score: 338.002
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 45..149
score: 14.219176
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 42..150

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh07G011690.1CmoCh07G011690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function GO:0009703 nitrate reductase (NADH) activity
molecular_function GO:0016491 oxidoreductase activity