CmoCh07G007170 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh07G007170
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionLipase_3 domain-containing protein
LocationCmo_Chr07: 3232681 .. 3234779 (+)
RNA-Seq ExpressionCmoCh07G007170
SyntenyCmoCh07G007170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCAGGGAGTTGTCCGAGATCTTGATCGCCACCCATGGCTGCCCGTCGTGCTTATAACTGTAACAACGGCTTCTCCGACAGCTATATGCTTCTGAAGCCGGAAGAGGAGAAATTCTTGGACCTGTGGCGCCTCCTGTTTTCAAGTAACCTCAAGAAAAGGAGATTTTTAGATTCGAGTCACGCCAGAGAATACAATTTCTGGCATAGATTCTTCATCTTTCTCTCTATTCTCGTCCTCAAGCTCCTCGGATTTCTTGCCCACCCACTCGCCTTGTTCGGCTTCTACCTGGAGTCCTCCCTCAACGTTTTGTCTGCCAACCACGGCTTTAGAGGCATCCTCCTCAATATCTTGCGATGTAATTCCCCCTCAGGTTTTGTTTTCCGTCTCAAATCCCTGTTTTCATACAAACCCAACTTTTTTTTCTTCTTCTTTATGCAGTGAAGTTGAAAATACCGGACAGCTCATCAGCAGAGTACCTGTCGTTGATTGGTCATTTGGATAGCAGAGTAACGTTGGACCAAAGCATAAAGCCTGGCGATGTCAACTACTTTGGAGCTCTCTGCATGATGGCTTCTAAAGTCGTCTATGAGAATGAAGCACATGTTCTACAGACAGTCAACGATGTCTGGAAGGTGCACTCCATCACTTACCAATTATTTAAATTTTAGAATAAATTTGTAAGAGCTAGTTGAAACAATTAGATTACCAATCACAAAGTCTAAACTTTTGAAACAAATTTTTAGTCAAATGTTTTGCTCTCTAACTGATAATTTTTGTTGTTTAAATGTAGATGGAGTTCTTGGAATTTTTCAACTTTTGGAACAGTAAGAAGAGAATTACTCCTTTTCTGCTTTGCTTCTCAAATATATAATTGTTCTGTTTGTAGGCAACGGCAACTGACATATAATTGATAATGGTGTTCTTATTTGTATTTACTAGACTATCAAGAAAAATGCTCAACACAAGCATTCATGATGCGGGATAAAAGGGTTGATCACGACACCATAATTGTGTCTTTCAGAGGCACAGAGCCCTTCAATGCCGATGACTGGAGCTCCGACTTCGACATCTCTTGGTACGAGATCGAAGGCATAGGAAAAATCCATGGTGGATTCATGAAAGCACTTGGGTTGCAGAAGACCCATGGATGGCCCAAGGAAATAGACAGAGAAGATAAAAGAAGGCCTTTGGCTTACTACACCCTAAGAGAGAGGCTAAGAGAACTTCTCAAAGAAGATGAGAGAACAAGGTTTGTAGTGACAGGGCACAGCCTGGGCGGCGCTTTGGCCATTCTATTCCCTTCTATTTTGGCGTTCCATGAAGAGAAGTTGCTGCTGGAAAGATTAGAGGGAGTTTATACATTTGGACAACCTCGAGTTGGAGACAGCAAATTCGGAGAATTCATGGTGAAATCACTGTCGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGTTTCGACATGGTCCCTCGGCTGCCCCTTGACAACAACACTTTGATGTACAAACACTTCGGTCCTTGCATTTATTTCAACTGGAACTATGTTGCGCAGGTAAATGAAGCTAACATTTTGGAATGAAACAAACCCTTTCTAACAACTTAAACAATATACTTTGTTTTTGCGCTTTCAGATTCTGGAAGAAGAGCCATTCAAGAACTATTTCTCAATCGTGGGAGCGGTACTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACAATTGGGTGGCGAAGGGGAAAGGAGTACGAGGAAAGAATGTGTTTTAGAATCGTGAGGTTGCTTGGTTTGTTATTGCCTGGAATTCCTGCTCATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGGTTCAACAGGTAGTCTTAACAGCGAGTATGATGTTAAAATTGAGTGAAGACTTTTCTTTTAAAAGAAAAAAAATTGCCATGTGATTTGAATGGCAGTTCAAAACATGGAGTACATCAATTTTAGCACCTGCCCCAAACCCAAAAGCCAGCTACAGTTTCTTATTTTACAACAATTGTACATTGCTTGCAACATCTAGGATGACACAGAACCAATCAAGCTACGCTGCTACTGTAAATCTCTAA

mRNA sequence

TCCAGGGAGTTGTCCGAGATCTTGATCGCCACCCATGGCTGCCCGTCGTGCTTATAACTGTAACAACGGCTTCTCCGACAGCTATATGCTTCTGAAGCCGGAAGAGGAGAAATTCTTGGACCTGTGGCGCCTCCTGTTTTCAAGTAACCTCAAGAAAAGGAGATTTTTAGATTCGAGTCACGCCAGAGAATACAATTTCTGGCATAGATTCTTCATCTTTCTCTCTATTCTCGTCCTCAAGCTCCTCGGATTTCTTGCCCACCCACTCGCCTTGTTCGGCTTCTACCTGGAGTCCTCCCTCAACGTTTTGTCTGCCAACCACGGCTTTAGAGGCATCCTCCTCAATATCTTGCGATTGAAGTTGAAAATACCGGACAGCTCATCAGCAGAGTACCTGTCGTTGATTGGTCATTTGGATAGCAGAGTAACGTTGGACCAAAGCATAAAGCCTGGCGATGTCAACTACTTTGGAGCTCTCTGCATGATGGCTTCTAAAGTCGTCTATGAGAATGAAGCACATGTTCTACAGACAGTCAACGATGTCTGGAAGATGGAGTTCTTGGAATTTTTCAACTTTTGGAACAACTATCAAGAAAAATGCTCAACACAAGCATTCATGATGCGGGATAAAAGGGTTGATCACGACACCATAATTGTGTCTTTCAGAGGCACAGAGCCCTTCAATGCCGATGACTGGAGCTCCGACTTCGACATCTCTTGGTACGAGATCGAAGGCATAGGAAAAATCCATGGTGGATTCATGAAAGCACTTGGGTTGCAGAAGACCCATGGATGGCCCAAGGAAATAGACAGAGAAGATAAAAGAAGGCCTTTGGCTTACTACACCCTAAGAGAGAGGCTAAGAGAACTTCTCAAAGAAGATGAGAGAACAAGGTTTGTAGTGACAGGGCACAGCCTGGGCGGCGCTTTGGCCATTCTATTCCCTTCTATTTTGGCGTTCCATGAAGAGAAGTTGCTGCTGGAAAGATTAGAGGGAGTTTATACATTTGGACAACCTCGAGTTGGAGACAGCAAATTCGGAGAATTCATGGTGAAATCACTGTCGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGTTTCGACATGGTCCCTCGGCTGCCCCTTGACAACAACACTTTGATGTACAAACACTTCGGTCCTTGCATTTATTTCAACTGGAACTATGTTGCGCAGATTCTGGAAGAAGAGCCATTCAAGAACTATTTCTCAATCGTGGGAGCGGTACTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACAATTGGGTGGCGAAGGGGAAAGGAGTACGAGGAAAGAATGTGTTTTAGAATCGTGAGGTTGCTTGGTTTGTTATTGCCTGGAATTCCTGCTCATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGGTTCAACAGCACCTGCCCCAAACCCAAAAGCCAGCTACAGTTTCTTATTTTACAACAATTGTACATTGCTTGCAACATCTAGGATGACACAGAACCAATCAAGCTACGCTGCTACTGTAAATCTCTAA

Coding sequence (CDS)

ATGGCTGCCCGTCGTGCTTATAACTGTAACAACGGCTTCTCCGACAGCTATATGCTTCTGAAGCCGGAAGAGGAGAAATTCTTGGACCTGTGGCGCCTCCTGTTTTCAAGTAACCTCAAGAAAAGGAGATTTTTAGATTCGAGTCACGCCAGAGAATACAATTTCTGGCATAGATTCTTCATCTTTCTCTCTATTCTCGTCCTCAAGCTCCTCGGATTTCTTGCCCACCCACTCGCCTTGTTCGGCTTCTACCTGGAGTCCTCCCTCAACGTTTTGTCTGCCAACCACGGCTTTAGAGGCATCCTCCTCAATATCTTGCGATTGAAGTTGAAAATACCGGACAGCTCATCAGCAGAGTACCTGTCGTTGATTGGTCATTTGGATAGCAGAGTAACGTTGGACCAAAGCATAAAGCCTGGCGATGTCAACTACTTTGGAGCTCTCTGCATGATGGCTTCTAAAGTCGTCTATGAGAATGAAGCACATGTTCTACAGACAGTCAACGATGTCTGGAAGATGGAGTTCTTGGAATTTTTCAACTTTTGGAACAACTATCAAGAAAAATGCTCAACACAAGCATTCATGATGCGGGATAAAAGGGTTGATCACGACACCATAATTGTGTCTTTCAGAGGCACAGAGCCCTTCAATGCCGATGACTGGAGCTCCGACTTCGACATCTCTTGGTACGAGATCGAAGGCATAGGAAAAATCCATGGTGGATTCATGAAAGCACTTGGGTTGCAGAAGACCCATGGATGGCCCAAGGAAATAGACAGAGAAGATAAAAGAAGGCCTTTGGCTTACTACACCCTAAGAGAGAGGCTAAGAGAACTTCTCAAAGAAGATGAGAGAACAAGGTTTGTAGTGACAGGGCACAGCCTGGGCGGCGCTTTGGCCATTCTATTCCCTTCTATTTTGGCGTTCCATGAAGAGAAGTTGCTGCTGGAAAGATTAGAGGGAGTTTATACATTTGGACAACCTCGAGTTGGAGACAGCAAATTCGGAGAATTCATGGTGAAATCACTGTCGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGTTTCGACATGGTCCCTCGGCTGCCCCTTGACAACAACACTTTGATGTACAAACACTTCGGTCCTTGCATTTATTTCAACTGGAACTATGTTGCGCAGATTCTGGAAGAAGAGCCATTCAAGAACTATTTCTCAATCGTGGGAGCGGTACTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACAATTGGGTGGCGAAGGGGAAAGGAGTACGAGGAAAGAATGTGTTTTAGAATCGTGAGGTTGCTTGGTTTGTTATTGCCTGGAATTCCTGCTCATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGGTTCAACAGCACCTGCCCCAAACCCAAAAGCCAGCTACAGTTTCTTATTTTACAACAATTGTACATTGCTTGCAACATCTAGGATGACACAGAACCAATCAAGCTACGCTGCTACTGTAAATCTCTAA

Protein sequence

MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTAPAPNPKASYSFLFYNNCTLLATSRMTQNQSSYAATVNL
Homology
BLAST of CmoCh07G007170 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 4.5e-102
Identity = 207/478 (43.31%), Postives = 279/478 (58.37%), Query Frame = 0

Query: 57  HRFFIFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSS 116
           HR+ IF+SI+V KL+     P+   G+ +E  LN+ S N  F G+L NIL  K+ +P   
Sbjct: 85  HRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGKVVMPHRG 144

Query: 117 SAEYLSLIGHLDSRVTLDQS----IKPGDVNYFGA----------LCMMASKVVYENEAH 176
           S  ++S IGHLD R+ L +S     + G+ +++            LCMMASK+ YENE  
Sbjct: 145 SETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEKV 204

Query: 177 VLQTVNDVWKMEFLEFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWS 236
           V   VN  WKM F++F+N WN+++++ STQ F++ DK  D + I+VSFRGTEPF+ADDW 
Sbjct: 205 VRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDWI 264

Query: 237 SDFDISWYEIEGIGKIHGGFMKALG-----------------------LQKTHGW--PKE 296
           +DFD SWYEI  +GK+H GF++ALG                       L+  H    P E
Sbjct: 265 TDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPSE 324

Query: 297 IDR-----------------EDKRRP---------------LAYYTLRERLRELLKEDER 356
             +                  D  RP                AYY +R +L+ LLKE + 
Sbjct: 325 SSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHKN 384

Query: 357 TRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQ 416
            +FVVTGHSLGGALAILFP++L  HEE  ++ERL G+YT+GQPRVG+ + G FM   L  
Sbjct: 385 AKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLEH 444

Query: 417 YKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAV 464
              +Y+R VY  D+VPRLP DN T ++KHFG C Y+N  Y+ Q + EEP  NYF +   V
Sbjct: 445 PVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFLV 504

BLAST of CmoCh07G007170 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 6.5e-101
Identity = 202/497 (40.64%), Postives = 297/497 (59.76%), Query Frame = 0

Query: 10  NNGFSDSYMLLKPEEEKFLDLWRL-LFSSNLKKRRFLDSSHAREYNFW--------HRFF 69
           N G   +Y++++P    ++DL+R  +      K +FL+    RE++          HR+ 
Sbjct: 12  NPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWV 71

Query: 70  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 129
           I +SILV K++  L  P+   GF ++  LN+ SAN GF G+LL +++ K+ IP+  S  +
Sbjct: 72  IVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTF 131

Query: 130 LSLIGHLDSRVTLDQ------------SIKPGDVNY-FGA-----LCMMASKVVYENEAH 189
           +S IG LD R++L +            S+  G V    G+     LC+MASK+ YEN   
Sbjct: 132 VSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKV 191

Query: 190 VLQTVNDVWKMEFLEFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWS 249
           V   V+  WKM  +EF + WN+YQ++ STQ F+  DK+ D + I++SFRGTEPF+ADDW 
Sbjct: 192 VENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWG 251

Query: 250 SDFDISWYEIEGIGKIHGGFMKALGLQKTHGW---------PKEIDREDKRRPL------ 309
           +DFD SWYE+  +GK+H GF++A+GL                   + E+ ++ L      
Sbjct: 252 TDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVER 311

Query: 310 -AYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFG 369
            AYY +R  L+ LL E E  RFVVTGHSLGGALAILFP++L  +EE  +++RL GVYTFG
Sbjct: 312 SAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFG 371

Query: 370 QPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGPCIYFNWNYV 429
           QPR+G+ + G FM   L+Q   RY+R VY  D+VPRLP D+ T +YKHFG C++++  Y 
Sbjct: 372 QPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYN 431

Query: 430 AQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKEYEERMCFRIVRLLGLLLP 464
               E+EP  N + +   +L  + A +E+ R  T+G+  G +Y+E     + RL+GL++P
Sbjct: 432 ETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIP 491

BLAST of CmoCh07G007170 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 6.5e-85
Identity = 192/512 (37.50%), Postives = 276/512 (53.91%), Query Frame = 0

Query: 14  SDSYMLLKPEEEKFLDLWRLLFSSNL-KKRRFLDSSHAR------EYNFWHRFFIFLSIL 73
           S S++++ P+E  FLDL++ +  S+L    +F  +S  R       Y+ W      +S+ 
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWP-----VSVF 68

Query: 74  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 133
           + K+L     P A+ G   +  LN    NHGF GIL NI  ++LKIP    A+++S IG+
Sbjct: 69  ICKILQLFQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLIRLKIPKRGEADFISTIGY 128

Query: 134 LDSRVTL------------------DQSIKPG-DVNYFGA--------LCMMASKVVYEN 193
           LDSR+ L                  + S+K G +    GA        LC+MASK+ YEN
Sbjct: 129 LDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYEN 188

Query: 194 EAHVLQTVNDVWKMEFLEFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNAD 253
              V + V + WKM F+  +   N +Q+  +T AF+  DK  D + I++SFRGT PF+  
Sbjct: 189 TKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIP 248

Query: 254 DWSSDFDISWYEIEGIGKIHGGFMKALGL--------------QKTHGWPKEIDREDKRR 313
           +W +DFD S   +   G +H GF++A+GL               K+ G   E+ +E +  
Sbjct: 249 NWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMA 308

Query: 314 P--------LAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLE 373
           P          Y+     L+ LLK+ +  +FVVTGHSLGGALAILF  IL   +E  +L+
Sbjct: 309 PDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLD 368

Query: 374 RLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGP 433
           RL  VYTFGQPR+G+   G FM   L+  + RY+R VY  DMVPR+P D+    ++HFG 
Sbjct: 369 RLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGT 428

Query: 434 CIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKEYEERMCFRI 470
           CIY++  +     +EEP +N F I  A+   I A +E+ RSF +    G EY+E    R+
Sbjct: 429 CIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRM 488

BLAST of CmoCh07G007170 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.0e-08
Identity = 53/175 (30.29%), Postives = 79/175 (45.14%), Query Frame = 0

Query: 206 IIVSFRGTEPFNADDWSSDFDISWYEIEGI---GKIHGGFMKALGLQKTHGWPKEIDRED 265
           I++SFRG+   + ++W  + +    EI  I    + H GF        T  W    D   
Sbjct: 98  IVLSFRGSR--SIENWIGNLNFDLKEINDICSGCRGHDGF--------TSSWRSVAD--- 157

Query: 266 KRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGV 325
                   TLR+++ + ++E    R V TGHSLGGALA +  + L  +   +       V
Sbjct: 158 --------TLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDI------DV 217

Query: 326 YTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGP 378
           +++G PRVG+  F EF+     Q     YR  +  D+VPRLP       Y H  P
Sbjct: 218 FSYGAPRVGNRAFAEFLT---VQTGGTLYRITHTNDIVPRLP--PREFGYSHSSP 240

BLAST of CmoCh07G007170 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 1.3e-08
Identity = 54/163 (33.13%), Postives = 75/163 (46.01%), Query Frame = 0

Query: 205 TIIVSFRGTEPFNADDWSSDF---DISWYEIEGIGKIHGGFMKALGLQKTHGWPKEIDRE 264
           TI + FRG+      +W +D     +S+  + G  K+H GF+ + G         E+  E
Sbjct: 168 TIYIVFRGSSSIR--NWIADLTFVPVSYPPVSGT-KVHKGFLDSYG---------EVQNE 227

Query: 265 DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEG 324
                     L   + +  K+    +  VTGHSLGGA A+L    L   EE L    L  
Sbjct: 228 ----------LVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNL-F 287

Query: 325 VYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 365
           +YT GQPRVGD  F  ++V +     I Y R V   D+VP LP
Sbjct: 288 LYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP 303

BLAST of CmoCh07G007170 vs. ExPASy TrEMBL
Match: A0A6J1HGB4 (uncharacterized protein LOC111463247 OS=Cucurbita moschata OX=3662 GN=LOC111463247 PE=4 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.4e-277
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 466
           IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 465

BLAST of CmoCh07G007170 vs. ExPASy TrEMBL
Match: A0A6J1KN75 (uncharacterized protein LOC111497186 OS=Cucurbita maxima OX=3661 GN=LOC111497186 PE=4 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.6e-272
Identity = 457/465 (98.28%), Postives = 461/465 (99.14%), Query Frame = 0

Query: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNC+NGFSDSYMLLKPEE KFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCSNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSI+VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFL FFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGW KEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWTKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFP+ILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPTILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 466
           IGWRRGKEYEERMCFRIVRL GLLLPGIPAHCPQDYVN+TRLGST
Sbjct: 421 IGWRRGKEYEERMCFRIVRLFGLLLPGIPAHCPQDYVNATRLGST 465

BLAST of CmoCh07G007170 vs. ExPASy TrEMBL
Match: A0A1S3B1H5 (uncharacterized protein LOC107990286 OS=Cucumis melo OX=3656 GN=LOC107990286 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 7.7e-238
Identity = 401/462 (86.80%), Postives = 426/462 (92.21%), Query Frame = 0

Query: 7   YNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIL 66
           YNCN GFS SYMLL PEE KFLDL RLLFSSNLKKRRF+DSSHARE+NFWHRFFIFLSI+
Sbjct: 17  YNCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIV 76

Query: 67  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 126
           VLKLL F   PLAL GF+LESSLN LS N GF GILLN  RL+LKIPDSSSAEYLS+IGH
Sbjct: 77  VLKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGH 136

Query: 127 LDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNYQ 186
           LDSRVTLD SIKPGDVNYFGALCMMASK+ YENEAHV Q V DVWKMEFL FFNFWN+YQ
Sbjct: 137 LDSRVTLDSSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQ 196

Query: 187 EKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 246
           EKCSTQAFMMRDK+VDHDTI+VSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL
Sbjct: 197 EKCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 256

Query: 247 GLQKTHGWPKEIDRE--DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFP 306
           GLQK  GWPKEI+R+  D+RRPLAYYTLRE+L+EL+KE+ERTRFVVTGHSLGGALAILFP
Sbjct: 257 GLQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFP 316

Query: 307 SILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 366
           SIL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLP
Sbjct: 317 SILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLP 376

Query: 367 LDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWR 426
           LD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTI WR
Sbjct: 377 LDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWR 436

Query: 427 RGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTA 467
           RGKEYEE++  RIVRL GLLLPGIPAHCPQDYVNSTRLGST+
Sbjct: 437 RGKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTS 478

BLAST of CmoCh07G007170 vs. ExPASy TrEMBL
Match: A0A5D3CNF4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004760 PE=4 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 8.5e-237
Identity = 400/462 (86.58%), Postives = 426/462 (92.21%), Query Frame = 0

Query: 7   YNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIL 66
           YNCN GFS SYMLL PEE KFLDL RLLFSSNLKKRRF+DSSHARE+NFWHRFFIFLSI+
Sbjct: 14  YNCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIV 73

Query: 67  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 126
           VLKLL F   PLAL GF+LESSLN LS N GF GILLN  RL+LKIPDSSSAEYLS+IGH
Sbjct: 74  VLKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGH 133

Query: 127 LDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNYQ 186
           LDSRVTLD+SIKPGDVNYFGALCMMASK+ YENEAHV Q V DVWKMEFL FFNFWN+YQ
Sbjct: 134 LDSRVTLDRSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQ 193

Query: 187 EKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 246
           EKCSTQAFMMRDK+VDHDTI+VSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL
Sbjct: 194 EKCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 253

Query: 247 GLQKTHGWPKEIDRE--DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFP 306
           GLQK  GWPKEI+R+  D+RRPLAYYTLRE+L+EL+KE+ERTRFVVTGHSLGGALAILFP
Sbjct: 254 GLQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFP 313

Query: 307 SILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 366
           SIL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLP
Sbjct: 314 SILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLP 373

Query: 367 LDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWR 426
           LD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTI WR
Sbjct: 374 LDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWR 433

Query: 427 RGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTA 467
           RGKEYEE++  RIVRL GLLLPGI AHCPQDYVNSTRLGST+
Sbjct: 434 RGKEYEEKVFLRIVRLFGLLLPGILAHCPQDYVNSTRLGSTS 475

BLAST of CmoCh07G007170 vs. ExPASy TrEMBL
Match: A0A0A0KFS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490940 PE=4 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.8e-234
Identity = 394/462 (85.28%), Postives = 426/462 (92.21%), Query Frame = 0

Query: 7   YNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIL 66
           YNCN GFS+SYMLL PEE KFLDL+RLLFSSNLKKRRF+DSSHARE+NFWHRFFIFLSI+
Sbjct: 16  YNCNGGFSNSYMLLNPEEVKFLDLFRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIV 75

Query: 67  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 126
           VLKLL F   PLAL GF+LESSLN LS N GF GILLN  RL+LKIP++SSAEYLS+IGH
Sbjct: 76  VLKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPNTSSAEYLSMIGH 135

Query: 127 LDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNYQ 186
           LDSRVTLD+SIKPGDVNYFGALCMMASK+ YENEAHV Q V DVWKMEFL FFNFWN+YQ
Sbjct: 136 LDSRVTLDRSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQ 195

Query: 187 EKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 246
           EKCSTQAFMMRD++ +HDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL
Sbjct: 196 EKCSTQAFMMRDRKDNHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 255

Query: 247 GLQKTHGWPKEIDRE-DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPS 306
           GLQK  GWPKE++R+  +R PLAYYTLRE+L+EL+KE+ERTRFVVTGHSLGGALAILFPS
Sbjct: 256 GLQKCVGWPKEMERKGHERHPLAYYTLREKLKELMKENERTRFVVTGHSLGGALAILFPS 315

Query: 307 ILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPL 366
           IL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL++YKIRYYRFVYGFDMVPRLPL
Sbjct: 316 ILVFHEEKLLLERLEGVYTFGQPRVGDRTFGEFMVKSLAEYKIRYYRFVYGFDMVPRLPL 375

Query: 367 DNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRR 426
           D+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTI WRR
Sbjct: 376 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRR 435

Query: 427 GKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTAP 468
           GKEYEE++  RIVRL GLLLPGIPAHCPQDYVNSTRLGST P
Sbjct: 436 GKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTTP 477

BLAST of CmoCh07G007170 vs. NCBI nr
Match: XP_022962880.1 (uncharacterized protein LOC111463247 [Cucurbita moschata])

HSP 1 Score: 962.2 bits (2486), Expect = 1.7e-276
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 466
           IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 465

BLAST of CmoCh07G007170 vs. NCBI nr
Match: KAG6595033.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027056.1 Lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 955.7 bits (2469), Expect = 1.6e-274
Identity = 462/465 (99.35%), Postives = 463/465 (99.57%), Query Frame = 0

Query: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNCNNGFSDSYMLLKPEE KFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSI+VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFL FFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 466
           IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 465

BLAST of CmoCh07G007170 vs. NCBI nr
Match: XP_023518931.1 (uncharacterized protein LOC111782304 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 949.9 bits (2454), Expect = 8.9e-273
Identity = 460/465 (98.92%), Postives = 462/465 (99.35%), Query Frame = 0

Query: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNCNNGFSDSYMLLKPEE KFLDLWRLLFSSNLKKRRFLDSS AREYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSRAREYNFWHRFF 60

Query: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSI+VLKLLGFLA+PLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSIVVLKLLGFLANPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFL FFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 466
           IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 465

BLAST of CmoCh07G007170 vs. NCBI nr
Match: XP_023003657.1 (uncharacterized protein LOC111497186 [Cucurbita maxima])

HSP 1 Score: 946.8 bits (2446), Expect = 7.5e-272
Identity = 457/465 (98.28%), Postives = 461/465 (99.14%), Query Frame = 0

Query: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNC+NGFSDSYMLLKPEE KFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCSNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSI+VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFL FFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGW KEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWTKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFP+ILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPTILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 466
           IGWRRGKEYEERMCFRIVRL GLLLPGIPAHCPQDYVN+TRLGST
Sbjct: 421 IGWRRGKEYEERMCFRIVRLFGLLLPGIPAHCPQDYVNATRLGST 465

BLAST of CmoCh07G007170 vs. NCBI nr
Match: XP_008440283.1 (PREDICTED: uncharacterized protein LOC107990286 [Cucumis melo])

HSP 1 Score: 832.8 bits (2150), Expect = 1.6e-237
Identity = 401/462 (86.80%), Postives = 426/462 (92.21%), Query Frame = 0

Query: 7   YNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIL 66
           YNCN GFS SYMLL PEE KFLDL RLLFSSNLKKRRF+DSSHARE+NFWHRFFIFLSI+
Sbjct: 17  YNCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIV 76

Query: 67  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 126
           VLKLL F   PLAL GF+LESSLN LS N GF GILLN  RL+LKIPDSSSAEYLS+IGH
Sbjct: 77  VLKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGH 136

Query: 127 LDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNYQ 186
           LDSRVTLD SIKPGDVNYFGALCMMASK+ YENEAHV Q V DVWKMEFL FFNFWN+YQ
Sbjct: 137 LDSRVTLDSSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQ 196

Query: 187 EKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 246
           EKCSTQAFMMRDK+VDHDTI+VSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL
Sbjct: 197 EKCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 256

Query: 247 GLQKTHGWPKEIDRE--DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFP 306
           GLQK  GWPKEI+R+  D+RRPLAYYTLRE+L+EL+KE+ERTRFVVTGHSLGGALAILFP
Sbjct: 257 GLQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFP 316

Query: 307 SILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 366
           SIL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLP
Sbjct: 317 SILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLP 376

Query: 367 LDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWR 426
           LD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTI WR
Sbjct: 377 LDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWR 436

Query: 427 RGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTA 467
           RGKEYEE++  RIVRL GLLLPGIPAHCPQDYVNSTRLGST+
Sbjct: 437 RGKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTS 478

BLAST of CmoCh07G007170 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 461.5 bits (1186), Expect = 8.9e-130
Identity = 230/454 (50.66%), Postives = 316/454 (69.60%), Query Frame = 0

Query: 17  YMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSILVLKLLGFLAH 76
           Y++L+PEE +  +L RLLFS +++K R +DSS   E++F HR+ IF+S+++LKLL F + 
Sbjct: 13  YLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHSFRHRWLIFVSLVLLKLLRFFSK 72

Query: 77  PLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGHLDSRVTLDQS 136
            LAL G  LE SLN LS N+ F G+    LR ++ +P  +S  Y S IGHLD+RV+LD +
Sbjct: 73  LLALVGSALEFSLNFLS-NNSFSGL---FLRGEVVMPQRTSENYQSFIGHLDTRVSLDMT 132

Query: 137 IKPGD-VNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNYQEKCSTQAFM 196
           +   D   Y+ AL +MASK+ YEN A +   V + W M++L   ++WN YQEK +TQAF+
Sbjct: 133 LNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVDYWNEYQEKETTQAFI 192

Query: 197 MRDKRV------DHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALGLQ 256
           M              T++V+FRGTE FN++DW SDFDI+W+E+  IG IHGGFMKALGLQ
Sbjct: 193 MSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPNIGNIHGGFMKALGLQ 252

Query: 257 KTHGWPKE-IDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILA 316
               WPKE +   D++ PLAYY++R+ L+ L+ +++ T+FV+TGHSLGGALAILF ++L 
Sbjct: 253 NNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGGALAILFTAVLV 312

Query: 317 FHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNN 376
            H E  LLER++GVYT+GQPRVGDSKFGEFM K L +Y I+YYRFVY  D+VPRLP D+ 
Sbjct: 313 IHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNNDIVPRLPYDDK 372

Query: 377 TLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKE 436
            LM+KHFG CIY++ NY A+++ E+  +N+F + G + M   A  E  RSFTI   +G E
Sbjct: 373 DLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIRSFTIVAEKGSE 432

Query: 437 YEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRL 463
           Y E    +  R LG+++PG+  H PQDYVN+TRL
Sbjct: 433 YSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRL 462

BLAST of CmoCh07G007170 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 436.0 bits (1120), Expect = 4.0e-122
Identity = 217/455 (47.69%), Postives = 311/455 (68.35%), Query Frame = 0

Query: 16  SYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYN----FWHRFFIFLSILVLKLL 75
           +Y +L P E    DL  LLFSS+L  R+F+ SS  R  +    F  R+ IF+SI++ KL+
Sbjct: 6   NYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQKLI 65

Query: 76  GFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGHLDSRV 135
                PL   GF L   LN+LS+N GF  IL N+ +  +  P+ +SA + SL G+LD RV
Sbjct: 66  ILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGNLDRRV 125

Query: 136 TLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNYQEKCST 195
            L+  ++ G   Y   L +MASK+ YEN   V   +++ WKM+ L F++ WN YQ++ ST
Sbjct: 126 ELNPKVERGSKRYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQKQRST 185

Query: 196 QAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALGLQKT 255
           +  +++D   D + IIVSFRGT+PF+ADDW +D D+SWYE++ +GKIHGGFMKALGLQK 
Sbjct: 186 EVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALGLQK- 245

Query: 256 HGWPKEIDREDKRRP---LAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILA 315
            GWPKE++ ++ +      AYYT+R  L+E+L ++  ++F++TGHSLGGALAILF ++L 
Sbjct: 246 EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFTAVLV 305

Query: 316 FHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNN 375
            H+E+ +LERLEGVYTFGQPRVGD +FG FM  SL ++ ++Y R+VY  DMVPRLP D+ 
Sbjct: 306 MHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLPFDDK 365

Query: 376 TLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKE 435
           TLM+KHFG C+Y++  Y  ++ EEEP KNYF++V  +   ++A +E+ RSF + + +G E
Sbjct: 366 TLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYWKGGE 425

Query: 436 YEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLG 464
           + E    R  R++ LL+PG+PAH P +Y+N T LG
Sbjct: 426 FREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLG 459

BLAST of CmoCh07G007170 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 2.5e-116
Identity = 216/461 (46.85%), Postives = 308/461 (66.81%), Query Frame = 0

Query: 16  SYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSS----HAREYNFWHRFFIFLSILVLKLL 75
           +Y +L P E    DL RLLF S+L+ R+F+D+S      R   F  R+ IF+SI+V KLL
Sbjct: 6   NYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKLL 65

Query: 76  GFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGHLDSRV 135
             L  PL+  GF L   LN+ S+N GF  I LN+++ +   P+ +SA + S+ G+LD +V
Sbjct: 66  IILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNLDQKV 125

Query: 136 T--LDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNYQEKC 195
              L +SIK GD  Y   L +MASK+ YENE  +   + D W+M+ L F++  N++ +  
Sbjct: 126 ELGLGRSIKIGDERYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFDQTR 185

Query: 196 STQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALGLQ 255
           ST+  ++RD + + + I+VSFRGT+PFNADDW +D D+SW+ +  +GKIHGGFMKALGL 
Sbjct: 186 STEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKALGLP 245

Query: 256 KTHGWPKEID---REDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSI 315
           K  GW +EI+    ++K   LAYYT+  +L+E+ +++  ++F+++GHSLGGALAILF ++
Sbjct: 246 K-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILFTAV 305

Query: 316 LAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLD 375
           L  H+EK +LERLEGVYTFGQPRVGD  FG +M   L ++ ++Y R+VY  DMVPRLP D
Sbjct: 306 LIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRLPFD 365

Query: 376 NNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRG 435
           + TLM+KHFG C+Y +  Y  ++ EEEP KNYF+I   +   I+A +E+ RSF I   +G
Sbjct: 366 DKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISCWQG 425

Query: 436 KEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTAP 468
           +EY E       RL+ LL+PG+PAH P +YVN   LG+  P
Sbjct: 426 REYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGNFPP 465

BLAST of CmoCh07G007170 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 410.6 bits (1054), Expect = 1.8e-114
Identity = 213/465 (45.81%), Postives = 294/465 (63.23%), Query Frame = 0

Query: 10  NNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSS----HAREYNFWHRFFIFLSI 69
           N  F +SY L+ P +  FLDL  LLFSSNL   RF+DS          +F  R+ + L+I
Sbjct: 5   NMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAI 64

Query: 70  LVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIG 129
            + K+L  L+ P A  G  L   LN+L+AN GF  ++LN++  KL  PD SSA Y S IG
Sbjct: 65  FLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIG 124

Query: 130 HLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFNFWNNY 189
             D R+ LD+ I  G + Y   L +MASK+ YE++ ++   V + WKM+ +  ++F+N +
Sbjct: 125 CSDRRIELDEKINVGSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 184

Query: 190 QEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKA 249
           QE   TQAF+ +    + D I+VSFRGTEPF A DW +D D+SWYE++ +GK+H GF +A
Sbjct: 185 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 244

Query: 250 LGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPS 309
           LGLQK  GWPKE          AYYT+R+ LR+ L  ++  ++++TGHSLGGALA LFP+
Sbjct: 245 LGLQK-DGWPKE--NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPA 304

Query: 310 ILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPL 369
           ILA H E  LL++LEG+YTFGQPRVGD  FGEFM   + ++ I Y RFVY  D+VPR+P 
Sbjct: 305 ILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPF 364

Query: 370 DNNTLM-YKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWR 429
           D+  L  YKH+GPC  FN  Y  ++ E+ P  NYF+++  +   +   +E  RSF + + 
Sbjct: 365 DDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQFW 424

Query: 430 RGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTAPAP 470
           +G EY+E    R VR++G++ PG   H P DYVNSTRLG     P
Sbjct: 425 KGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPP 466

BLAST of CmoCh07G007170 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 369.4 bits (947), Expect = 4.6e-102
Identity = 202/497 (40.64%), Postives = 297/497 (59.76%), Query Frame = 0

Query: 10  NNGFSDSYMLLKPEEEKFLDLWRL-LFSSNLKKRRFLDSSHAREYNFW--------HRFF 69
           N G   +Y++++P    ++DL+R  +      K +FL+    RE++          HR+ 
Sbjct: 12  NPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWV 71

Query: 70  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 129
           I +SILV K++  L  P+   GF ++  LN+ SAN GF G+LL +++ K+ IP+  S  +
Sbjct: 72  IVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTF 131

Query: 130 LSLIGHLDSRVTLDQ------------SIKPGDVNY-FGA-----LCMMASKVVYENEAH 189
           +S IG LD R++L +            S+  G V    G+     LC+MASK+ YEN   
Sbjct: 132 VSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKV 191

Query: 190 VLQTVNDVWKMEFLEFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWS 249
           V   V+  WKM  +EF + WN+YQ++ STQ F+  DK+ D + I++SFRGTEPF+ADDW 
Sbjct: 192 VENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWG 251

Query: 250 SDFDISWYEIEGIGKIHGGFMKALGLQKTHGW---------PKEIDREDKRRPL------ 309
           +DFD SWYE+  +GK+H GF++A+GL                   + E+ ++ L      
Sbjct: 252 TDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVER 311

Query: 310 -AYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFG 369
            AYY +R  L+ LL E E  RFVVTGHSLGGALAILFP++L  +EE  +++RL GVYTFG
Sbjct: 312 SAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFG 371

Query: 370 QPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGPCIYFNWNYV 429
           QPR+G+ + G FM   L+Q   RY+R VY  D+VPRLP D+ T +YKHFG C++++  Y 
Sbjct: 372 QPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYN 431

Query: 430 AQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKEYEERMCFRIVRLLGLLLP 464
               E+EP  N + +   +L  + A +E+ R  T+G+  G +Y+E     + RL+GL++P
Sbjct: 432 ETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIP 491

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1S3ZP854.5e-10243.31Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1[more]
F4JFU86.5e-10140.64Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
Q5VKJ76.5e-8537.50Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1[more]
O599521.0e-0830.29Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1[more]
P195151.3e-0833.13Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1HGB48.4e-277100.00uncharacterized protein LOC111463247 OS=Cucurbita moschata OX=3662 GN=LOC1114632... [more]
A0A6J1KN753.6e-27298.28uncharacterized protein LOC111497186 OS=Cucurbita maxima OX=3661 GN=LOC111497186... [more]
A0A1S3B1H57.7e-23886.80uncharacterized protein LOC107990286 OS=Cucumis melo OX=3656 GN=LOC107990286 PE=... [more]
A0A5D3CNF48.5e-23786.58Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0KFS71.8e-23485.28Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490940 PE... [more]
Match NameE-valueIdentityDescription
XP_022962880.11.7e-276100.00uncharacterized protein LOC111463247 [Cucurbita moschata][more]
KAG6595033.11.6e-27499.35Lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027056.1 Lipase, pa... [more]
XP_023518931.18.9e-27398.92uncharacterized protein LOC111782304 [Cucurbita pepo subsp. pepo][more]
XP_023003657.17.5e-27298.28uncharacterized protein LOC111497186 [Cucurbita maxima][more]
XP_008440283.11.6e-23786.80PREDICTED: uncharacterized protein LOC107990286 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G67050.18.9e-13050.66alpha/beta-Hydrolases superfamily protein [more]
AT5G42930.14.0e-12247.69alpha/beta-Hydrolases superfamily protein [more]
AT1G56630.12.5e-11646.85alpha/beta-Hydrolases superfamily protein [more]
AT1G45201.11.8e-11445.81triacylglycerol lipase-like 1 [more]
AT3G14360.14.6e-10240.64alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 499..504
NoneNo IPR availablePANTHERPTHR46086:SF17ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 9..466
NoneNo IPR availableCDDcd00519Lipase_3coord: 148..382
e-value: 4.99491E-48
score: 163.801
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 207..367
e-value: 5.0E-38
score: 130.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 128..405
e-value: 6.8E-48
score: 165.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 148..405
IPR044819Triacylglycerol lipase OBL1-likePANTHERPTHR46086ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 9..466

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh07G007170.1CmoCh07G007170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004806 triglyceride lipase activity