CmoCh07G006050 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh07G006050
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptiontRNA-dihydrouridine synthase
LocationCmo_Chr07: 2723227 .. 2723697 (-)
RNA-Seq ExpressionCmoCh07G006050
SyntenyCmoCh07G006050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCTACCAAGCATGCTCAGAGGGCAGACTACTGTGAGAGGCCGCTTGTTTATAAGTAAAGGAGGCAGTTGGTATCATTTTGGGTTGACACTGAATTGTATTACATCAGTTAGAAAGACAAAAGGAAAAATAAAAAATAAAAAATACCAATAAAAATACCAAGTGATACTTCTGTAAGTTTTGTATGTGCTCTTTGTTTTCATTTTATTTTAATATGTTTTTGTAGTCGTGCTCGATAACTTGCAGAAGAGTTGCAGATAGGCCCAGAGATCCAGCTAAGTGGAGTGAGATTGCAGATGTCGTGGCAGCATTGTCTGTCCCGGTTATTGCGAACGGTGATATCTTTGAGTACGAAGATTTTCACCATATCAAAGTTGCTACAGGTTTGCTCAAAAGGCACTGTTTTATGGATTTCTTGATGATGCCTGAATTTCTTTTAGTGAATGGGTTGAATATGATCCCAACCTGA

mRNA sequence

ATGTGCTACCAAGCATGCTCAGAGGGCAGACTACTAAGAGTTGCAGATAGGCCCAGAGATCCAGCTAAGTGGAGTGAGATTGCAGATGTCGTGGCAGCATTGTCTGTCCCGGTTATTGCGAACGGTGATATCTTTGAGTACGAAGATTTTCACCATATCAAAGTTGCTACAGGTTTGCTCAAAAGGCACTGTTTTATGGATTTCTTGATGATGCCTGAATTTCTTTTAGTGAATGGGTTGAATATGATCCCAACCTGA

Coding sequence (CDS)

ATGTGCTACCAAGCATGCTCAGAGGGCAGACTACTAAGAGTTGCAGATAGGCCCAGAGATCCAGCTAAGTGGAGTGAGATTGCAGATGTCGTGGCAGCATTGTCTGTCCCGGTTATTGCGAACGGTGATATCTTTGAGTACGAAGATTTTCACCATATCAAAGTTGCTACAGGTTTGCTCAAAAGGCACTGTTTTATGGATTTCTTGATGATGCCTGAATTTCTTTTAGTGAATGGGTTGAATATGATCCCAACCTGA

Protein sequence

MCYQACSEGRLLRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGLLKRHCFMDFLMMPEFLLVNGLNMIPT
Homology
[Close]
BLAST of CmoCh07G006050 vs. ExPASy Swiss-Prot
Match: O68273 (tRNA-dihydrouridine(16) synthase OS=Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) OX=381666 GN=dusC PE=3 SV=2)

HSP 1 Score: 44.3 bits (103), Expect = 8.1e-04
Identity = 26/64 (40.62%), Postives = 34/64 (53.12%), Query Frame = 0 

Query: 2   CYQACSEG--RLLRVADRPRD-----PAKWSEIADVVAALSVPVIANGDIFEYEDFHHIK 59
           C  A +EG    L V  R RD     PA W  IA + AA+ VPVIANGD++   D+   +
Sbjct: 154 CATALAEGGAASLVVHARTRDHGYRPPAHWDWIARIAAAVDVPVIANGDVWTVADWERCR 213

[Close]
BLAST of CmoCh07G006050 vs. ExPASy TrEMBL
Match: A0A6J1KSI8 (tRNA-dihydrouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497209 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.7e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 180 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 225

[Close]
BLAST of CmoCh07G006050 vs. ExPASy TrEMBL
Match: A0A6J1KNB4 (tRNA-dihydrouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497209 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.7e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 156 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 201

[Close]
BLAST of CmoCh07G006050 vs. ExPASy TrEMBL
Match: A0A6J1HFN6 (tRNA-dihydrouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111463056 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.7e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 156 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 201

[Close]
BLAST of CmoCh07G006050 vs. ExPASy TrEMBL
Match: A0A6J1HHN5 (tRNA-dihydrouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111463056 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.7e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 180 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 225

[Close]
BLAST of CmoCh07G006050 vs. ExPASy TrEMBL
Match: A0A5B6YTB2 (Putative tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (Fragment) OS=Davidia involucrata OX=16924 GN=Din_004545 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.6e-16
Identity = 42/46 (91.30%), Postives = 45/46 (97.83%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           +VADRPRDPAKWSEIADVVAALS+PVIANGD+FEYEDF HIKVATG
Sbjct: 174 KVADRPRDPAKWSEIADVVAALSIPVIANGDVFEYEDFQHIKVATG 219

[Close]
BLAST of CmoCh07G006050 vs. NCBI nr
Match: KAG7026892.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 98.2 bits (243), Expect = 3.6e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 183 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 228

[Close]
BLAST of CmoCh07G006050 vs. NCBI nr
Match: XP_022962639.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 98.2 bits (243), Expect = 3.6e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 180 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 225

[Close]
BLAST of CmoCh07G006050 vs. NCBI nr
Match: XP_023003695.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 98.2 bits (243), Expect = 3.6e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 180 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 225

[Close]
BLAST of CmoCh07G006050 vs. NCBI nr
Match: XP_023517247.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 98.2 bits (243), Expect = 3.6e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 156 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 201

[Close]
BLAST of CmoCh07G006050 vs. NCBI nr
Match: XP_023517246.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 98.2 bits (243), Expect = 3.6e-17
Identity = 46/46 (100.00%), Postives = 46/46 (100.00%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Sbjct: 180 RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 225

[Close]
BLAST of CmoCh07G006050 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 82.8 bits (203), Expect = 1.5e-16
Identity = 36/46 (78.26%), Postives = 41/46 (89.13%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           ++ADRPRDPAKW EIADVVAALS+PVIANGD+ EY+DF  IK ATG
Sbjct: 180 KIADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATG 225

[Close]
BLAST of CmoCh07G006050 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 82.8 bits (203), Expect = 1.5e-16
Identity = 36/46 (78.26%), Postives = 41/46 (89.13%), Query Frame = 0 

Query: 13  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG 59
           ++ADRPRDPAKW EIADVVAALS+PVIANGD+ EY+DF  IK ATG
Sbjct: 141 KIADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATG 186

The following BLAST results are available for this feature:
BLAST of CmoCh07G006050 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O682738.1e-0440.63tRNA-dihydrouridine(16) synthase OS=Cupriavidus necator (strain ATCC 17699 / DSM... [more]
BLAST of CmoCh07G006050 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KSI81.7e-17100.00tRNA-dihydrouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497209 PE=3 SV... [more]
A0A6J1KNB41.7e-17100.00tRNA-dihydrouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497209 PE=3 SV... [more]
A0A6J1HFN61.7e-17100.00tRNA-dihydrouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111463056 PE=3 ... [more]
A0A6J1HHN51.7e-17100.00tRNA-dihydrouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111463056 PE=3 ... [more]
A0A5B6YTB25.6e-1691.30Putative tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (Fragment) OS=Davidia i... [more]
BLAST of CmoCh07G006050 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7026892.13.6e-17100.00tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein [Cucurbita argyrosperma ... [more]
XP_022962639.13.6e-17100.00tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita moschata][more]
XP_023003695.13.6e-17100.00tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita maxima][more]
XP_023517247.13.6e-17100.00tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X2 [Cucurbita pepo subsp... [more]
XP_023517246.13.6e-17100.00tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita pepo subsp... [more]
BLAST of CmoCh07G006050 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G49640.11.5e-1678.26Aldolase-type TIM barrel family protein [more]
AT3G49640.21.5e-1678.26Aldolase-type TIM barrel family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035587DUS-like, FMN-binding domainPFAMPF01207Duscoord: 18..58
e-value: 1.7E-7
score: 30.7
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 9..72
e-value: 3.0E-8
score: 35.3
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 16..58

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh07G006050.1CmoCh07G006050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002943 tRNA dihydrouridine synthesis
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity