CmoCh07G002650 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh07G002650
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	sphingosine kinase 1
Location	Cmo_Chr07: 1303717 .. 1309312 (-)
RNA-Seq Expression	CmoCh07G002650
Synteny	CmoCh07G002650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCAATCCGGAGTTCCAGAGCCTGTCCTTAACCACCGTCTCCTCCTTGACGACGGAATCGTCACGCCAATGATTCTCACAGCCGACGGTTGGCTTCGGTGGTCGGAGAAAGGCCAACGATCTCTGGCCATTGAGAAAGAGGTAATGGGATTCTCCATGGATGGTCCAAAGATTAGGATCAAAGCTCTCGTTAAGGACCGTGGTGGATTGTGGTGCTTTGGAAGCAGCGGAGCTTTGGTGAGGAAGGAGTTTGTGTTTCAGCCCCGTGAATGCATCGATTTGCTTGGTAACACGATCGTCCAGAATTCTGTCTGGTTCTAATATTGACTTCGAGTAGTTTAGAAAATGCATTGAAAGGGGGAGAAGGATTTTGCTGTGATCTGATTTGTTTGTGCCTGCTGATTGCCATGATGATTTAATGTTTTCGTTTCTCTCCTAGATCTCTCAAGAGCCAAACGGAAACTCATTATCTAATACTTCGTTCTTTATGCTGTTCATTCGGCTTAATTTCGAGAACAAGAGAAATCTTCTTATTGCGATTCTTCTCATTGCAAATTCCCGTCGTCACATGTTAGTTACGGAATAGGATGAATAATGTCCGTCAGTTTTTCTGCTGATTAAGAGTTGGAGAACACTCCAGGATCATCGGCGTTTTTAGTGCTTCAAACTTGTTCTGCGTAGTGTTCCTTTCCATTGTTTTCTGGAATAATGGCAACTCTGTTTCAAACTTGGTCTTTTTTGTTTGCTTAGGTGCCTTTATCTTTCTTTTCCCCCTATTTCCACGTTCTTACAAGCATCACCTTGTTTAGTAGGTAATAAACGGATTGCTAGGGCGAGATGATTGGGTTGATGCAATAAAGATGCCTTTAGGAGTAGTGCCTGCAGGTATCGTATTCTTAGCTTGTTCGTCTTTTTTTAGTATTACAACAAGAATTTGAACTTACTACATCTGAAGGAGATGTTTAAATGATATGCAGACTGGAATTGTAATGACCATGGATCATATCTAGTGAAAGAACATTCGTGTCTGATGGCACTCGATTTCTCAGATTTCCCCCTTAACCAACGAAAAGACACTCCCATTCTATTACCATTTATTGGAGTTCCTTGTTTGGAACAACAAGAATTCTGATAGTATTTACTGGGGTTCATATATGCATTGGCACTGGAAATGGAATGGTGATATCACTTCTTCATTCAAATGGTGGCCTTTGTACCCCATGCGATGCTACTCTAGCCATCCTTCAAGGTTAGTATAGTTTTGTCAATCTCCAATGTTCATCTTATTTTCCCTTATGATCGAGCTATTTTGCAAACAAATTTTAATTTCTTGCAGGCCATAAATGTTCATTGGATGTTCCCACCGTTTTGCAAGGAGAGGCTAAACATTTTTCTGTGTTGATGCTTGCTTGGGGTATCTCTAACTTTATCTCTCTTTAGTGTTACTGCTGGGTAGTTGAGGTTTATTTGTCCTTTTGCCGATGCAACTATTTCTGCCGAGTCACCTGTTGTTTGCTTCTGCAGAGTTATCTAAGTTGAGTACTTAGGAATGCTACAAGTTACTACTATATTTTATATGCGTGTTCAGAGATGACATATAATGCCATTATTTTATATTAAAGATGACATATAACTGTCCTTGATTTTCAAACATTATTCATTTGGATTGGTTTGAAGTGCTTCTGTTACAGACCAAGGACGCAATACAAGCAAAGAGTCCCCTAACACACAGATCATACCAAAATTAAGGTTATCCGTGACCTGGTTTAGATGTTAAAAGTCCAAGTTCACAAAATCAAATCTTCTTCATTGGCATGCTTCTTGTTAGGAATCACGAATCTTTGCAATGGTATGATATTGTCCACCTTAAACACGAGCTCTCGTGGCTTTACTTTGAACTTTCTCGTAAGGCCTCATAATGGTCTAATCTTAATGGCTGTGCTTAAGCTTGCTGTCGAAACAACTCGCGTGCTGTCTGTGGCATAGGCTTCTTGATCCCATGGCCGCATGCTCTAAGTCTGTATTTAGTTCAAAGGCTTCTGTTTTTGTATCTTGAGAAGTATTCAAGGAGCCAAATTGGTAAGTGGAAGATTGTTTTCAGTCCTGTCTCTGCTGTACAATTGGTGTGAAACAAGTCGGTGAACCTGGTAGATTGTGTGTGTACAAAAATGATTTGAATCTTAATGTGTTGAAAGTAAAATCTTGATATGTTAGACAGGAAAAGAGAGGCCCGAAGAAAACACATGCTTTTGCAAGCTACCAACTACTTTTACTTCTAGGTGTACAAAAGGCACGCGGATAATTTATTCTCGTGCTAAATCCTTCCAACTTTGTACCAAAAAACATAATTTCTTGTGCATACTCTCCATTGTTTCCACCTCCGTACCTTTGATTCAAGTCGTTTTTACGAGCCATCTGATTAAAATTTTATTTGATGGCGTGTATGAGATATTTATTTATAGTGCTTTATTGGTAGTGAGTCCACTTTGACTAATTTATTAAATTTGAAGTAAATGAAAAGTTGCCAACCCAGCCAACGAATGCTAGCCACCTTCCAATAATTTTTTTCTATATACTATTTATCTTTACCTAAATCTTAAAGCCCAACAAATATATAATTAAAAAAAAAAGGTTGACTTCTCCCATTGAGAAGATAAATCATAATAATTGCAAAATGTTTATATGACATAATTATATTACTAGTTGGACTAATTGAAATATTCAAAGTGAATCGACCAACTAAATTATATTTAATGTCATTTTATAGGTTGGTTTAATTAAATATTAGACATAAAATTCAATAAACTTTTAATTTGTAACGATTGGGAAAGTGAAGATAATAATTTTATTTTATATTTTAAATAAGTTTTTTCATTTTTATTCCAACTCTGATGCTCATCTGTTGAAGTTTCTTTTTTATTTGGCTTTGTCTTCCAAGAACAAAAAAACAAAATAATTTGTTTTAGTTTTCATATTAATTAAAAAAAAAAAACATACACACCGAGACAAAGTTACATAAATATTTTAAAGTCGAAATAAAACAAAATCTATTTTTCATTTCGGACTCGTAATTTATTTTAGTTAGTAGTGAAAACTATTTAACTTTTAATATTCATTAATAAATAAATAAATAAATAAAACAAAAACAGAGTTTCATGAGAGAAAAATATTTCAAAATTTAAATAAAACAAAACCTGTTTTTTATTTATGACTGTTATAATGATACGACATAATTTGTTTTAGTTAGTAGCAAAAACGGTTTAACTTTTAATATTAACTTAAAAAATAAAATAAATAAAATAGAGACAAAGTTACATATGGGAAAATATTTCAAAATTTAATTAAAATAACACCCGTTTTTCATTTCTTACAGTTATAATGAGACCACAAAATTTGTTTTAGTTAGTAGTGAAAACTGTTTAACTTTTAATATTCATTGAAACAGATAAAATAAATAAGACAGAGGCAGAGTTAGAAAAATATTTCAAAATTTAAATAAAACAAAATATGTTTTTTATTTCTGACTGTTATAATGACACGATATAATTTGTTTTTCGTTAATAATAAAAACCGTTTAACTTTTAATATTAATTGAAAAAAAAAACAAATAAAACGGTCACAGAATTACATATGAGAAAATATTTTAAAGTTTGAATAAAATAAAACCTGTTTTTCATTTATAACCGTTATAGTGACGCCACATCATTTATTTTAATTATTAGGAAAACTGTTTCACTATTAATATTAACTGAAAAAAATAAAATAAATAAAAACAAAGGTCAAGTTATATGAGGAAAAATATTTCAAAATTTAAATAAAACAAAATCTACTTTTTCATTTTTTATTGTTATAATTACATGACATAATTTATATTAGTGAGCGGTAGAAAATGGTTTAACTTTTAATATTAATTAAAAAATAAAATAAATAAAACAACATGTAGAAAAATATTTCAAAGTTTTAATGAAAACATTTTTTTATTGTTACAATGACACGACATAATTTGTTTTAGTTAGTAGTAAAAATCGTTTAACTTTTAATATTTATTAAAAAAAATAAAACAAATATAGAGTTTCATAGAAAAAAAATACTTCAAAATTTGAATAAAACAAAACGACATAATTTGTTTTAATTAATAATGAAAACTGTTTAATTTTTAATATTAATTGAAAAAATAAAATAAATAAGAGAGAGAGAGAGATACGTGGAGAGAAAATATTTCAAAATTTGAATAAAACAAAGCCTAATTTTACATTTCTAACTATTAAAATGACACGACATTCTTCTCCAAATACCTTCAACTTTGTATGGGTGGGTCCACTTAATTTGTAATTTATCATTTGTTTTTTTTCTTCATAAAAGTATATAAGTATTACTCTAAGAAGTGCTCATTTTATTCGTGGGTACCCGCCCCTTGAACGTGAAGGAGATCATAAGTTTAAACGCACACGCACACATATATATTTCAGTTGAAAAATTAGAGAAAAAAAAAACATAATTAACTATTAAACAAACTCTTTTCAATGAGGAAAAATGTAATAATAATAGTTTATAAGTAACAATAAAATAAAATGAAATAAATGTCCCATTTAAGTAATATTGAAACATACCAATCAAAAGTAAAATAAAGATTTGTATACGTATGATATTAATATCAATTTATAAATTAAAACTGCATTTAATTCTAAAAAACCGTATTTAAAGTTTAAAAATATATAATTAAAAAACACAGCTTTATTACTATATTCAATTGTGGCTGCCACAAAAGGTTAAATTATTTAATATATCAATTTAAAAAGTTCGCTGACCTCTACCACAAGGGCACTTAAGTAAATAAAAAATAAAGATATTTTTTAAATTTCAAATTTTATAGATATTTTTTTAATATTATTTAAAGATTTTTATGCATATTTTTAAAGACATTTAAACTCTTAGAAGGTATTTATGAAATATTATTAAAATTATAATTCTTTCGTTTTTAAAAATTTAAGGATATTTTTTAGATAAATTATAAAGTTTAAGAGCGCTTTTGGTTAATTTAACCTATATATTACTATATATATCCTCATCGTCATTTTATTTTATCGTTGAAAATGATATAATATTTGTAAAATAATAAGAAGTACCAACCACTATTTGATTTAAAAAAAAAAATGCACACACTATTTGATTTGAGCTCAAAAAATAAAAAGAGATGATTAATTAAAGAAATATAAAATAAGTGAGCAATAAAAAAATAAAAAAGAAAAGAAAAGAGGGAAGGACGGCAAATAATAAGGACAGAACACGAGTCGACGTGATTTTGAAGCCTCGTGGATTGATTGTATAATACAGTGAAAATAAAGAGAGAAAATAATAATAATAATAATAATTTGGATGTTATTTTCTTATGTTAATTTTGGTTGGTTTAAATACGTCGTTGTCTTCTCTCCAAACCCTCTTCTGGTCTGATACACATCCTGGGTCGCTTCTCGTCCCATGATTATTGCAGATCAGGTCGCGGCAACCACCATTACTCCTCTTCTCGCTTTCTTCTTCTTCTTCCGTTTCTCTCCTTTCAGACCTCTCCTTCCTCTATCACTTCCCTTTCTAG

mRNA sequence

ATGGATCAATCCGGAGTTCCAGAGCCTGTCCTTAACCACCGTCTCCTCCTTGACGACGGAATCGTCACGCCAATGATTCTCACAGCCGACGGTTGGCTTCGGTGGTCGGAGAAAGGCCAACGATCTCTGGCCATTGAGAAAGAGGTAATGGGATTCTCCATGGATGGTCCAAAGATTAGGATCAAAGCTCTCGTTAAGGACCGTGGTGGATTGTGGTGCTTTGGAAGCAGCGGAGCTTTGGTGAGGAAGGAGTTTGTGTTTCAGCCCCGTGAATGCATCGATTTGCTTGGGCGAGATGATTGGGTTGATGCAATAAAGATGCCTTTAGGAGTAGTGCCTGCAGACTGGAATTTATTTACTGGGGTTCATATATGCATTGGCACTGGAAATGGAATGGTGATATCACTTCTTCATTCAAATGGTGGCCTTTGTACCCCATGCGATGCTACTCTAGCCATCCTTCAAGGCCATAAATGTTCATTGGATGTTCCCACCGTTTTGCAAGGAGAGGCTAAACATTTTTCTGTGTTGATGCTTGCTTGGGCTTGCTGTCGAAACAACTCGCGTGCTGTCTGTGGCATAGGCTTCTTGATCCCATGGCCGCATGCTCTAAGTCTGTATTTAGTTCAAAGGCTTCTGTTTTTGTATCTTGAGAAGTATTCAAGGAGCCAAATTGGTAAGTGGAAGATTGTTTTCAGTCCTGTCTCTGCTGTACAATTGATCAGGTCGCGGCAACCACCATTACTCCTCTTCTCGCTTTCTTCTTCTTCTTCCGTTTCTCTCCTTTCAGACCTCTCCTTCCTCTATCACTTCCCTTTCTAG

Coding sequence (CDS)

ATGGATCAATCCGGAGTTCCAGAGCCTGTCCTTAACCACCGTCTCCTCCTTGACGACGGAATCGTCACGCCAATGATTCTCACAGCCGACGGTTGGCTTCGGTGGTCGGAGAAAGGCCAACGATCTCTGGCCATTGAGAAAGAGGTAATGGGATTCTCCATGGATGGTCCAAAGATTAGGATCAAAGCTCTCGTTAAGGACCGTGGTGGATTGTGGTGCTTTGGAAGCAGCGGAGCTTTGGTGAGGAAGGAGTTTGTGTTTCAGCCCCGTGAATGCATCGATTTGCTTGGGCGAGATGATTGGGTTGATGCAATAAAGATGCCTTTAGGAGTAGTGCCTGCAGACTGGAATTTATTTACTGGGGTTCATATATGCATTGGCACTGGAAATGGAATGGTGATATCACTTCTTCATTCAAATGGTGGCCTTTGTACCCCATGCGATGCTACTCTAGCCATCCTTCAAGGCCATAAATGTTCATTGGATGTTCCCACCGTTTTGCAAGGAGAGGCTAAACATTTTTCTGTGTTGATGCTTGCTTGGGCTTGCTGTCGAAACAACTCGCGTGCTGTCTGTGGCATAGGCTTCTTGATCCCATGGCCGCATGCTCTAAGTCTGTATTTAGTTCAAAGGCTTCTGTTTTTGTATCTTGAGAAGTATTCAAGGAGCCAAATTGGTAAGTGGAAGATTGTTTTCAGTCCTGTCTCTGCTGTACAATTGATCAGGTCGCGGCAACCACCATTACTCCTCTTCTCGCTTTCTTCTTCTTCTTCCGTTTCTCTCCTTTCAGACCTCTCCTTCCTCTATCACTTCCCTTTCTAG

Protein sequence

MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIRIKALVKDRGGLWCFGSSGALVRKEFVFQPRECIDLLGRDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTPCDATLAILQGHKCSLDVPTVLQGEAKHFSVLMLAWACCRNNSRAVCGIGFLIPWPHALSLYLVQRLLFLYLEKYSRSQIGKWKIVFSPVSAVQLIRSRQPPLLLFSLSSSSSVSLLSDLSFLYHFPF

Homology

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BLAST of CmoCh07G002650 vs. ExPASy Swiss-Prot
Match: Q8L7L1 (Sphingosine kinase 1 OS=Arabidopsis thaliana OX=3702 GN=SPHK1 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.2e-26
Identity = 80/270 (29.63%), Postives = 114/270 (42.22%), Query Frame = 0


Query: 6   VPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIRIKALV 65
           +P P +    +L +G+VTP+ LTA+G LR +E G+R   + KE++ F ++G K+R+K LV
Sbjct: 12  LPSPAIISDRVLVNGVVTPLTLTAEGELRSTESGRRKSTLAKEILSFVVEGNKVRVKTLV 71

Query: 66  KDRGGLWCFGSSGALVRKEFVFQPRE---------------------------------- 125
           +  GG+ C GS+G   R +FVF+P                                    
Sbjct: 72  EKGGGICCRGSAGDYARNDFVFEPLSDESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGK 131

Query: 126 -------------------------------------------------CID-------- 182
                                                            C+         
Sbjct: 132 KTARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKYDGIVCVSGDGILVEV 191

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BLAST of CmoCh07G002650 vs. ExPASy Swiss-Prot
Match: F2Y4A3 (Sphingosine kinase 2 OS=Arabidopsis thaliana OX=3702 GN=SPHK2 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.1e-25
Identity = 85/274 (31.02%), Postives = 121/274 (44.16%), Query Frame = 0


Query: 2   DQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRS-LAIEKEVMGFSMDGPKIR 61
           DQ   P  ++   ++  DG +  + LTADG LRW+E G R  L+++K+V+GF ++G +IR
Sbjct: 4   DQFMCPTDIIT-GIVFIDGELAMLTLTADGELRWTEYGLRQYLSMKKDVLGFIVEGKQIR 63

Query: 62  IKALV-KDRGGLWCFGSSGALVRKEFVFQP-----------RECIDLLGRD--------- 121
           +KA+V K+ GG+ C    G  VRK+FVF+P           R+ +D LGR          
Sbjct: 64  VKAVVEKEAGGICCGQFGGDFVRKDFVFEPLIDQNGWCYKLRQYLDSLGRPKRLLVFVNP 123

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 124 FGGKKSAREIFVKEVKPLFEDADVQLEIQETKYQLHAKEFVKSMDVSKYDGIVCVSGDGI 183

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BLAST of CmoCh07G002650 vs. ExPASy TrEMBL
Match: A0A0A0KK35 (DAGKc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517070 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 4.5e-59
Identity = 136/275 (49.45%), Postives = 151/275 (54.91%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQSG  + +LNHR+LL DG VTPMILT DGWL+WSEK QR L+++KEV+GFSMDGPKIR
Sbjct: 1   MDQSGDSDFILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSVDKEVLGFSMDGPKIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+D GGL CFGSSGALVRKEFVFQP             RECIDL+G          
Sbjct: 61  IKALVEDHGGLRCFGSSGALVRKEFVFQPLSEESRALWCLKLRECIDLIGRPKKLFVLVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGKGTGSKIYRDEVKPILEDAEIDVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDWVDAIK PLGVVPA            GTGNGMV SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMVKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. ExPASy TrEMBL
Match: A0A5A7UAC8 (Sphingosine kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003250 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.1e-57
Identity = 136/275 (49.45%), Postives = 150/275 (54.55%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQS   + +LNHR+LL DG VTPMILT DGWL+WSEK QR L+I+KEV+GFSMDGPKIR
Sbjct: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+DRGGL CFG+SGALVRKEFVFQP             RECIDLLG          
Sbjct: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDWVDAIK PLGVVPA            GTGNGMV SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMVKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. ExPASy TrEMBL
Match: A0A1S3B0H4 (sphingosine kinase 1 OS=Cucumis melo OX=3656 GN=LOC103484546 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.1e-57
Identity = 136/275 (49.45%), Postives = 150/275 (54.55%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQS   + +LNHR+LL DG VTPMILT DGWL+WSEK QR L+I+KEV+GFSMDGPKIR
Sbjct: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+DRGGL CFG+SGALVRKEFVFQP             RECIDLLG          
Sbjct: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDWVDAIK PLGVVPA            GTGNGMV SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMVKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. ExPASy TrEMBL
Match: A0A6J1IMH9 (sphingosine kinase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478387 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.9e-57
Identity = 134/275 (48.73%), Postives = 148/275 (53.82%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQSG  +P+LNHR+LL DG VTPMILTADG LRW E+GQRSL+++KEV+GFS DGP IR
Sbjct: 1   MDQSGDSKPILNHRVLLPDGSVTPMILTADGCLRWLEEGQRSLSVDKEVLGFSTDGPNIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+D GGL CFGSSG LVRKEFVFQP             REC+DLLG          
Sbjct: 61  IKALVEDLGGLLCFGSSGGLVRKEFVFQPLSEESRALWCLKLRECVDLLGRPKRLFVFVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGRRAGYKIYLDDVKPILDDAEIVVTLQETKYQRHAEEVAYSLDLTEYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDWVDAIK PLGVVPA            GTGNGM  SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMAKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. ExPASy TrEMBL
Match: A0A6J1IKY1 (sphingosine kinase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478387 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.9e-57
Identity = 134/275 (48.73%), Postives = 148/275 (53.82%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQSG  +P+LNHR+LL DG VTPMILTADG LRW E+GQRSL+++KEV+GFS DGP IR
Sbjct: 1   MDQSGDSKPILNHRVLLPDGSVTPMILTADGCLRWLEEGQRSLSVDKEVLGFSTDGPNIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+D GGL CFGSSG LVRKEFVFQP             REC+DLLG          
Sbjct: 61  IKALVEDLGGLLCFGSSGGLVRKEFVFQPLSEESRALWCLKLRECVDLLGRPKRLFVFVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGRRAGYKIYLDDVKPILDDAEIVVTLQETKYQRHAEEVAYSLDLTEYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDWVDAIK PLGVVPA            GTGNGM  SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMAKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. NCBI nr
Match: XP_038883381.1 (sphingosine kinase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 245.0 bits (624), Expect = 7.6e-61
Identity = 139/275 (50.55%), Postives = 152/275 (55.27%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQSG  E VLNHR+LL DGIVTP+ILT+DGWLRWSEKGQR L++EKEV+GFSMDGPKIR
Sbjct: 1   MDQSGDSESVLNHRVLLPDGIVTPLILTSDGWLRWSEKGQRRLSVEKEVLGFSMDGPKIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+DRGGL CFGS+GAL RKEFVFQP             RECIDLLG          
Sbjct: 61  IKALVEDRGGLRCFGSNGALTRKEFVFQPLSEESRALWCLKLRECIDLLGRPKRLFVLVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGKRAGCKIYLDNVKPILEDAEIDVTMQETKYQRHAEEVAYSLDLTKYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDW DAIKMPLGVVPA            GTGNGM  SLLH  G  CT 
Sbjct: 181 ILVEVINGLLRRDDWADAIKMPLGVVPA------------GTGNGMAKSLLHLTGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. NCBI nr
Match: XP_004134960.1 (sphingosine kinase 1 [Cucumis sativus] >KGN49199.1 hypothetical protein Csa_004342 [Cucumis sativus])

HSP 1 Score: 238.0 bits (606), Expect = 9.4e-59
Identity = 136/275 (49.45%), Postives = 151/275 (54.91%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQSG  + +LNHR+LL DG VTPMILT DGWL+WSEK QR L+++KEV+GFSMDGPKIR
Sbjct: 1   MDQSGDSDFILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSVDKEVLGFSMDGPKIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+D GGL CFGSSGALVRKEFVFQP             RECIDL+G          
Sbjct: 61  IKALVEDHGGLRCFGSSGALVRKEFVFQPLSEESRALWCLKLRECIDLIGRPKKLFVLVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGKGTGSKIYRDEVKPILEDAEIDVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDWVDAIK PLGVVPA            GTGNGMV SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMVKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. NCBI nr
Match: KAG7033898.1 (Sphingosine kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 233.8 bits (595), Expect = 1.8e-57
Identity = 134/277 (48.38%), Postives = 148/277 (53.43%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQSG  +P+LNHR+LL DG VTPMILTADG LRW E+GQRSL+++KEV+GFS DGP IR
Sbjct: 1   MDQSGDSKPILNHRVLLPDGSVTPMILTADGCLRWLEEGQRSLSVDKEVLGFSTDGPNIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+D GGL CFGSSG LVRKEFVFQP             REC+DLLG          
Sbjct: 61  IKALVEDLGGLLCFGSSGGLVRKEFVFQPLSEESRALWCLKLRECVDLLGRPKRLFVFVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGRRAGYKIYLDDVKPILDDAEIVVTLQETKYQRHAEEVAYSLDITEYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 184
                      RDDWVDAIK PLGVVPA            GTGNGM  SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMAKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. NCBI nr
Match: XP_008439896.1 (PREDICTED: sphingosine kinase 1 [Cucumis melo] >KAA0052712.1 sphingosine kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 233.4 bits (594), Expect = 2.3e-57
Identity = 136/275 (49.45%), Postives = 150/275 (54.55%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQS   + +LNHR+LL DG VTPMILT DGWL+WSEK QR L+I+KEV+GFSMDGPKIR
Sbjct: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+DRGGL CFG+SGALVRKEFVFQP             RECIDLLG          
Sbjct: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDWVDAIK PLGVVPA            GTGNGMV SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMVKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. NCBI nr
Match: XP_022978376.1 (sphingosine kinase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 232.6 bits (592), Expect = 3.9e-57
Identity = 134/275 (48.73%), Postives = 148/275 (53.82%), Query Frame = 0


Query: 1   MDQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIR 60
           MDQSG  +P+LNHR+LL DG VTPMILTADG LRW E+GQRSL+++KEV+GFS DGP IR
Sbjct: 1   MDQSGDSKPILNHRVLLPDGSVTPMILTADGCLRWLEEGQRSLSVDKEVLGFSTDGPNIR 60

Query: 61  IKALVKDRGGLWCFGSSGALVRKEFVFQP-------------RECIDLLG---------- 120
           IKALV+D GGL CFGSSG LVRKEFVFQP             REC+DLLG          
Sbjct: 61  IKALVEDLGGLLCFGSSGGLVRKEFVFQPLSEESRALWCLKLRECVDLLGRPKRLFVFVN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 PFGGRRAGYKIYLDDVKPILDDAEIVVTLQETKYQRHAEEVAYSLDLTEYDGIVCVSGDG 180

Query: 181 -----------RDDWVDAIKMPLGVVPADWNLFTGVHICIGTGNGMVISLLHSNGGLCTP 182
                      RDDWVDAIK PLGVVPA            GTGNGM  SLLHS G  CT 
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPA------------GTGNGMAKSLLHSIGDPCTA 240

[Close]

BLAST of CmoCh07G002650 vs. TAIR 10
Match: AT4G21540.1 (sphingosine kinase 1 )

HSP 1 Score: 120.9 bits (302), Expect = 1.6e-27
Identity = 80/270 (29.63%), Postives = 114/270 (42.22%), Query Frame = 0


Query: 6   VPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIRIKALV 65
           +P P +    +L +G+VTP+ LTA+G LR +E G+R   + KE++ F ++G K+R+K LV
Sbjct: 12  LPSPAIISDRVLVNGVVTPLTLTAEGELRSTESGRRKSTLAKEILSFVVEGNKVRVKTLV 71

Query: 66  KDRGGLWCFGSSGALVRKEFVFQPRE---------------------------------- 125
           +  GG+ C GS+G   R +FVF+P                                    
Sbjct: 72  EKGGGICCRGSAGDYARNDFVFEPLSDESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGK 131

Query: 126 -------------------------------------------------CID-------- 182
                                                            C+         
Sbjct: 132 KTARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKYDGIVCVSGDGILVEV 191

[Close]

BLAST of CmoCh07G002650 vs. TAIR 10
Match: AT4G21540.3 (sphingosine kinase 1 )

HSP 1 Score: 120.9 bits (302), Expect = 1.6e-27
Identity = 80/270 (29.63%), Postives = 114/270 (42.22%), Query Frame = 0


Query: 6   VPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRSLAIEKEVMGFSMDGPKIRIKALV 65
           +P P +    +L +G+VTP+ LTA+G LR +E G+R   + KE++ F ++G K+R+K LV
Sbjct: 12  LPSPAIISDRVLVNGVVTPLTLTAEGELRSTESGRRKSTLAKEILSFVVEGNKVRVKTLV 71

Query: 66  KDRGGLWCFGSSGALVRKEFVFQPRE---------------------------------- 125
           +  GG+ C GS+G   R +FVF+P                                    
Sbjct: 72  EKGGGICCRGSAGDYARNDFVFEPLSDESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGK 131

Query: 126 -------------------------------------------------CID-------- 182
                                                            C+         
Sbjct: 132 KTARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKYDGIVCVSGDGILVEV 191

[Close]

BLAST of CmoCh07G002650 vs. TAIR 10
Match: AT4G21534.1 (Diacylglycerol kinase family protein )

HSP 1 Score: 118.6 bits (296), Expect = 7.7e-27
Identity = 85/274 (31.02%), Postives = 121/274 (44.16%), Query Frame = 0


Query: 2   DQSGVPEPVLNHRLLLDDGIVTPMILTADGWLRWSEKGQRS-LAIEKEVMGFSMDGPKIR 61
           DQ   P  ++   ++  DG +  + LTADG LRW+E G R  L+++K+V+GF ++G +IR
Sbjct: 4   DQFMCPTDIIT-GIVFIDGELAMLTLTADGELRWTEYGLRQYLSMKKDVLGFIVEGKQIR 63

Query: 62  IKALV-KDRGGLWCFGSSGALVRKEFVFQP-----------RECIDLLGRD--------- 121
           +KA+V K+ GG+ C    G  VRK+FVF+P           R+ +D LGR          
Sbjct: 64  VKAVVEKEAGGICCGQFGGDFVRKDFVFEPLIDQNGWCYKLRQYLDSLGRPKRLLVFVNP 123

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 124 FGGKKSAREIFVKEVKPLFEDADVQLEIQETKYQLHAKEFVKSMDVSKYDGIVCVSGDGI 183

[Close]

BLAST of CmoCh07G002650 vs. TAIR 10
Match: AT4G21540.2 (sphingosine kinase 1 )

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-09
Identity = 30/55 (54.55%), Postives = 38/55 (69.09%), Query Frame = 0


Query: 127 GTGNGMVISLLHSNGGLCTPCDATLAILQGHKCSLDVPTVLQGEAKHFSVLMLAW 182
           G+GNGM+ SLL   G  C+   AT++I++G   SLDV T+ QG  K FSVLMLAW
Sbjct: 5   GSGNGMIKSLLEPVGLPCSATSATISIIRGRTRSLDVATISQGTTKFFSVLMLAW 59

The following BLAST results are available for this feature:

BLAST of CmoCh07G002650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L7L1	2.2e-26	29.63	Sphingosine kinase 1 OS=Arabidopsis thaliana OX=3702 GN=SPHK1 PE=1 SV=1	[more]
F2Y4A3	1.1e-25	31.02	Sphingosine kinase 2 OS=Arabidopsis thaliana OX=3702 GN=SPHK2 PE=1 SV=1	[more]

BLAST of CmoCh07G002650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KK35	4.5e-59	49.45	DAGKc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517070 PE=4 ...	[more]
A0A5A7UAC8	1.1e-57	49.45	Sphingosine kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120...	[more]
A0A1S3B0H4	1.1e-57	49.45	sphingosine kinase 1 OS=Cucumis melo OX=3656 GN=LOC103484546 PE=4 SV=1	[more]
A0A6J1IMH9	1.9e-57	48.73	sphingosine kinase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478387 PE=4...	[more]
A0A6J1IKY1	1.9e-57	48.73	sphingosine kinase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478387 PE=4...	[more]

BLAST of CmoCh07G002650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883381.1	7.6e-61	50.55	sphingosine kinase 1 isoform X1 [Benincasa hispida]	[more]
XP_004134960.1	9.4e-59	49.45	sphingosine kinase 1 [Cucumis sativus] >KGN49199.1 hypothetical protein Csa_0043...	[more]
KAG7033898.1	1.8e-57	48.38	Sphingosine kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_008439896.1	2.3e-57	49.45	PREDICTED: sphingosine kinase 1 [Cucumis melo] >KAA0052712.1 sphingosine kinase ...	[more]
XP_022978376.1	3.9e-57	48.73	sphingosine kinase 1 isoform X1 [Cucurbita maxima]	[more]

BLAST of CmoCh07G002650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21540.1	1.6e-27	29.63	sphingosine kinase 1	[more]
AT4G21540.3	1.6e-27	29.63	sphingosine kinase 1	[more]
AT4G21534.1	7.7e-27	31.02	Diacylglycerol kinase family protein	[more]
AT4G21540.2	2.5e-09	54.55	sphingosine kinase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 95..183
None	No IPR available	PANTHER	PTHR12358:SF83	SPHINGOSINE KINASE 1-LIKE ISOFORM X1	coord: 8..89
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 8..89
None	No IPR available	PANTHER	PTHR12358:SF83	SPHINGOSINE KINASE 1-LIKE ISOFORM X1	coord: 95..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh07G002650.1	CmoCh07G002650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity