CmoCh07G001060 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh07G001060
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	gibberellin 2-beta-dioxygenase-like
Location	Cmo_Chr07: 610842 .. 612073 (-)
RNA-Seq Expression	CmoCh07G001060
Synteny	CmoCh07G001060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATTCTTTATTAAGAAAAACCATATAATCCGCCATGGTTGTTCTGTCTCAGCCTGCATTGGATTTAGCCAAATATTCTCTTCTCCGAGCAACATGTAAACCCGCCGTCGCCGCCGCCGCCGCGGATATTCCGGCAATCGACCTCGCAGACCCTGACGCGAAGTCCCACATCGTCAAGGCTTGCGAAGAAATTGGCTTCTTTAAGGTGGTAAGCCATCGAGTTCCCATCGAATTGATGACCAAACTGGAAGATGAAGCCCTCCGTTTTTTCAGTCTTTCTCAGGCTGAGAAAGATAAAGCTCGTCCCCCTTACCCTTTGGGCTATGGCTCCAAAAATATCGGCCCTAATGGCGATAAGGGGTGGATTGAATATTTCCTCCTAAATGCAAATCCTCTCCCTCTTTTCGCCCCAAACTCCAAATCTTTAATGTAAGTTACACAAAATTGAGCACTCTGTTTCTTTGAATTTAACCCAAATTTGTAAAATAAAATATATTTTTCATAGAATTTGAAAATTTATGCTAATTTTTGTGTAGCGCTGCCGTGAAGGAGTACGTGACGGCGGTGAAGAAGCTGAGCTGCGAAGTGGCGGAGCTAATAGCAGAGGGATTAAAAATCGAGCCACGAAACACAATAAGCAAGCTTCTAAATGAGGAGAAAAGCGATTGTTGTTTCAGAGTCAACCGGTATCCGCCGTGTCCGGAGCTCCAAGCTGTTGGTGGAAAAAGCATGATCGGGTTCGGGGAACACACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCGACCGGTCTACAAATCTGCCTGAGAGACGGCGCGTGGGTTTCAGTGCCACCAGACGCAGCGGCGTTCTTCGTGAACGTGGGCGACGCGCTGCAGGTGATGAGCAACGGCAGATTCCGAAGCGTGAAGCACAAAGTGGTGGCGGATTGTAACATCGAGAGGGTTTCAATGATTTACTTTGGAGGGCCGGCGTTGAGTGAAAAGATTGCGCCATTGCCACAGATTTTGGGAGACGGAGAAGAGAGCTTGTACAAGGAATTCTACTGGAGGGAATATAAAACGGCGGCGTACAAGACTAGATTGGCTGATTACAGGCTAGGCCCCTTTGAGAAGAATCAAAATTCGGAGCGGTTTTAATTCCATTCCATAATTGGAAACTCGTGCTAGCTTTCAAATTCTGTTTTAATCCCTTTTTTATTTGCTCAAGGATAGGCACTTTGGTT

mRNA sequence

CAATTCTTTATTAAGAAAAACCATATAATCCGCCATGGTTGTTCTGTCTCAGCCTGCATTGGATTTAGCCAAATATTCTCTTCTCCGAGCAACATGTAAACCCGCCGTCGCCGCCGCCGCCGCGGATATTCCGGCAATCGACCTCGCAGACCCTGACGCGAAGTCCCACATCGTCAAGGCTTGCGAAGAAATTGGCTTCTTTAAGGTGGTAAGCCATCGAGTTCCCATCGAATTGATGACCAAACTGGAAGATGAAGCCCTCCGTTTTTTCAGTCTTTCTCAGGCTGAGAAAGATAAAGCTCGTCCCCCTTACCCTTTGGGCTATGGCTCCAAAAATATCGGCCCTAATGGCGATAAGGGGTGGATTGAATATTTCCTCCTAAATGCAAATCCTCTCCCTCTTTTCGCCCCAAACTCCAAATCTTTAATCGCTGCCGTGAAGGAGTACGTGACGGCGGTGAAGAAGCTGAGCTGCGAAGTGGCGGAGCTAATAGCAGAGGGATTAAAAATCGAGCCACGAAACACAATAAGCAAGCTTCTAAATGAGGAGAAAAGCGATTGTTGTTTCAGAGTCAACCGGTATCCGCCGTGTCCGGAGCTCCAAGCTGTTGGTGGAAAAAGCATGATCGGGTTCGGGGAACACACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCGACCGGTCTACAAATCTGCCTGAGAGACGGCGCGTGGGTTTCAGTGCCACCAGACGCAGCGGCGTTCTTCGTGAACGTGGGCGACGCGCTGCAGGTGATGAGCAACGGCAGATTCCGAAGCGTGAAGCACAAAGTGGTGGCGGATTGTAACATCGAGAGGGTTTCAATGATTTACTTTGGAGGGCCGGCGTTGAGTGAAAAGATTGCGCCATTGCCACAGATTTTGGGAGACGGAGAAGAGAGCTTGTACAAGGAATTCTACTGGAGGGAATATAAAACGGCGGCGTACAAGACTAGATTGGCTGATTACAGGCTAGGCCCCTTTGAGAAGAATCAAAATTCGGAGCGGTTTTAATTCCATTCCATAATTGGAAACTCGTGCTAGCTTTCAAATTCTGTTTTAATCCCTTTTTTATTTGCTCAAGGATAGGCACTTTGGTT

Coding sequence (CDS)

ATGGTTGTTCTGTCTCAGCCTGCATTGGATTTAGCCAAATATTCTCTTCTCCGAGCAACATGTAAACCCGCCGTCGCCGCCGCCGCCGCGGATATTCCGGCAATCGACCTCGCAGACCCTGACGCGAAGTCCCACATCGTCAAGGCTTGCGAAGAAATTGGCTTCTTTAAGGTGGTAAGCCATCGAGTTCCCATCGAATTGATGACCAAACTGGAAGATGAAGCCCTCCGTTTTTTCAGTCTTTCTCAGGCTGAGAAAGATAAAGCTCGTCCCCCTTACCCTTTGGGCTATGGCTCCAAAAATATCGGCCCTAATGGCGATAAGGGGTGGATTGAATATTTCCTCCTAAATGCAAATCCTCTCCCTCTTTTCGCCCCAAACTCCAAATCTTTAATCGCTGCCGTGAAGGAGTACGTGACGGCGGTGAAGAAGCTGAGCTGCGAAGTGGCGGAGCTAATAGCAGAGGGATTAAAAATCGAGCCACGAAACACAATAAGCAAGCTTCTAAATGAGGAGAAAAGCGATTGTTGTTTCAGAGTCAACCGGTATCCGCCGTGTCCGGAGCTCCAAGCTGTTGGTGGAAAAAGCATGATCGGGTTCGGGGAACACACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCGACCGGTCTACAAATCTGCCTGAGAGACGGCGCGTGGGTTTCAGTGCCACCAGACGCAGCGGCGTTCTTCGTGAACGTGGGCGACGCGCTGCAGGTGATGAGCAACGGCAGATTCCGAAGCGTGAAGCACAAAGTGGTGGCGGATTGTAACATCGAGAGGGTTTCAATGATTTACTTTGGAGGGCCGGCGTTGAGTGAAAAGATTGCGCCATTGCCACAGATTTTGGGAGACGGAGAAGAGAGCTTGTACAAGGAATTCTACTGGAGGGAATATAAAACGGCGGCGTACAAGACTAGATTGGCTGATTACAGGCTAGGCCCCTTTGAGAAGAATCAAAATTCGGAGCGGTTTTAA

Protein sequence

MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVSHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANPLPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF

Homology

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BLAST of CmoCh07G001060 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.8e-114
Identity = 208/336 (61.90%), Postives = 253/336 (75.30%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAA-ADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MVVLSQPAL+  ++ LL    KP  +      IP +DL  PDAK+ IV AC + GFFK+V
Sbjct: 1   MVVLSQPALN--QFFLL----KPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLV 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNAN 120
           +H VP+ELM  LE+EALRFF  SQ+EKD+A PP P GYGSK IGPNGD GW+EY LLN N
Sbjct: 61  NHGVPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTN 120

Query: 121 P-------LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
           P       L +F  N     A V+ Y+TAVK +   V EL+AEGL I  RNT+S+LL +E
Sbjct: 121 PDVISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDE 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           KSD CFR+N YPPCPE+QA+  ++++GFGEHTDPQIIS+LRSN+++GLQICL DG WVSV
Sbjct: 181 KSDSCFRLNHYPPCPEVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSV 240

Query: 241 PPDAAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
           PPD  +FF+NVGDALQVM+NGRF+SVKH+V+AD    R+SMIYFGGPALSE IAPLP ++
Sbjct: 241 PPDQTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVM 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEKN 329
             GEE LYKEF W EYK AAY +RLAD RL PF+K+
Sbjct: 301 LKGEECLYKEFTWCEYKKAAYTSRLADNRLAPFQKS 329

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BLAST of CmoCh07G001060 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 6.4e-105
Identity = 192/335 (57.31%), Postives = 250/335 (74.63%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVL QP + L  +  L  T KP     +  IP ++LADP+AK+ IVKACEE GFFKVV+
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           H V  ELMT+LE EA+ FF L Q+ K++A PP P GYG+K IGPNGD GWIEY LLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLI--------AAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
             L +P + ++          +V+EY+  +K++S +V E++AE L IEPR+T+SK+L +E
Sbjct: 121 -QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDE 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           KSD C R+N YP   E      K  +GFGEHTDPQIIS+LRSNN+ GLQIC++DG+WV+V
Sbjct: 181 KSDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAV 240

Query: 241 PPDAAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
           PPD ++FF+NVGDALQVM+NGRF+SVKH+V+AD    R+SMIYFGGP LS+KIAPLP ++
Sbjct: 241 PPDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLV 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
            + ++ LYKEF W +YK++AYK++L DYRLG FEK
Sbjct: 301 PEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of CmoCh07G001060 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 8.7e-102
Identity = 181/330 (54.85%), Postives = 249/330 (75.45%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MV+LS+P  +  +Y+ +R          ++ IP +DL+ PDAK+ IVKACE+ GFFKV++
Sbjct: 1   MVLLSKPTSE--QYTYVRNNMP---ITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVIN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           H +P++ +++LE EA +FFSL Q EK+KA P  P GYG+K IG NGD GWIEY LL  N 
Sbjct: 61  HGIPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQ 120

Query: 121 ---LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCC 180
                L+  +       +K+Y  A++ ++CE+ +L+AEGLKI+P+N  SKL+ +++SDC 
Sbjct: 121 DHNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCL 180

Query: 181 FRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAA 240
           FRVN YP CPEL A+ G+++IGFGEHTDPQIIS+LRSNN++G QI LRDG+W+SVPPD +
Sbjct: 181 FRVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHS 240

Query: 241 AFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEE 300
           +FF+NVGD+LQVM+NGRF+SV+H+V+A+    R+SMIYF GP LSEKIAPLP ++  G+E
Sbjct: 241 SFFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKE 300

Query: 301 SLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
           SLYKEF W EYK++ Y +RLAD RLG +E+
Sbjct: 301 SLYKEFTWFEYKSSTYGSRLADNRLGNYER 323

[Close]

BLAST of CmoCh07G001060 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.7e-94
Identity = 178/335 (53.13%), Postives = 228/335 (68.06%), Query Frame = 0


Query: 1   MVVLSQPA-LDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MV++ QPA  D   Y  +   CKP        IP IDL D DAK+ IVKACEE GFFKV+
Sbjct: 1   MVIVLQPASFDSNLY--VNPKCKPRPVL----IPVIDLTDSDAKTQIVKACEEFGFFKVI 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNAN 120
           +H V  +L+T+LE EA+ FF+L  + KDKA PP P GYG+K IGPNGD GW+EY LLNAN
Sbjct: 61  NHGVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNAN 120

Query: 121 -------PLPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
                     +F         AV+EY+  +K++S +  E++ E LKIEP+  +S+L+  +
Sbjct: 121 LCLESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVK 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           +SD C R+N Y   PE +    K  IGFGEHTDPQ+ISLLRSN++ GLQIC++DG WV V
Sbjct: 181 ESDSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDV 240

Query: 241 PPDAAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
            PD ++FFV VGD LQVM+NGRF+SVKH+VV +    R+SMIYF GP LSEKIAPL  ++
Sbjct: 241 TPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLV 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
              ++ LY EF W +YK +AYKT+L DYRLG FEK
Sbjct: 301 PKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of CmoCh07G001060 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.7e-94
Identity = 181/332 (54.52%), Postives = 230/332 (69.28%), Query Frame = 0


Query: 1   MVVLS-QPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MVVL+  PA+D     LLR+   P      + +P +DL  P A   +V ACE  GFFKVV
Sbjct: 1   MVVLAGPPAVD--HIPLLRS---PDPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVV 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNAN 120
           +H V  + M K E EA+RFFS +Q +KD++ P YP GYGSK IG NGD GW+EY LL  +
Sbjct: 61  NHGVATDTMDKAESEAVRFFSQTQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALD 120

Query: 121 PLPL----FAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSD 180
              L      P+     AA+ EY++ V+K++  V E ++EGL I   + +S L+  E SD
Sbjct: 121 DASLADACTVPSCAVFRAALNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSD 180

Query: 181 CCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPD 240
             FRVN YPPC  LQ + G S+ GFGEHTDPQ++S+LRSN ++GLQI LRDG WVSVP D
Sbjct: 181 QVFRVNHYPPCRALQGL-GCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSD 240

Query: 241 AAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDG 300
             +FFVNVGD+LQV++NGRF+SVKH+VVA+    RVS IYFGGP L+++IAPLPQ+LG+G
Sbjct: 241 RDSFFVNVGDSLQVLTNGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEG 300

Query: 301 EESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
           E+SLYKEF W EYK AAYK+RL D RL  FEK
Sbjct: 301 EQSLYKEFTWDEYKKAAYKSRLGDNRLAQFEK 326

[Close]

BLAST of CmoCh07G001060 vs. ExPASy TrEMBL
Match: A0A6J1EF07 (gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111433728 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.8e-190
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP
Sbjct: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF
Sbjct: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF 335
           KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF
Sbjct: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF 334

[Close]

BLAST of CmoCh07G001060 vs. ExPASy TrEMBL
Match: A0A6J1KTC6 (gibberellin 2-beta-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497054 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.4e-179
Identity = 319/333 (95.80%), Postives = 323/333 (97.00%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKP  AAAAA+IPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKP--AAAAANIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           H+VPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEY LLNANP
Sbjct: 61  HQVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLF PNS+SLIAAVKEYVTAVKKLSCEVAELIAE LKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFVPNSESLIAAVKEYVTAVKKLSCEVAELIAEELKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           NRYPPCPELQAV GKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDG WVSVPPDAAAFF
Sbjct: 181 NRYPPCPELQAVDGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGEWVSVPPDAAAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVAD N ERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADSNNERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSER 334
           KEFYWREYKTAAYKT+LADYRLGPFEKNQN ER
Sbjct: 301 KEFYWREYKTAAYKTKLADYRLGPFEKNQNLER 331

[Close]

BLAST of CmoCh07G001060 vs. ExPASy TrEMBL
Match: A0A2H4V3T7 (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.2e-157
Identity = 279/333 (83.78%), Postives = 303/333 (90.99%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPAL L +YSLLR TCKP   A+  +IP IDL+DP+A+SHIV+ACEE GFFK+V+
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKP--TASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           HRVPIELMTKLE+EAL FF LSQ+EKDKARPP PLGYG KNIG NGDKGWIEY LL ANP
Sbjct: 61  HRVPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LP+F+ NS+S +AA KEYVTAVKKLSCEVAELIAEGLKIEPRN ISKLLN+EKSDCCFRV
Sbjct: 121 LPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRV 180

Query: 181 NRYPPCPELQAVG--GKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAA 240
           N YPPCP+LQA+G  G++MIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAA
Sbjct: 181 NHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAA 240

Query: 241 FFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEES 300
            FVNVGD+LQVMSNGRF+SVKHKVVAD N ERVSMIYFGGP LSEKIAPLP++LGDGEES
Sbjct: 241 IFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEES 300

Query: 301 LYKEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           LYKEF W EYKTAAYKTRLADYRLGPFEKNQNS
Sbjct: 301 LYKEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of CmoCh07G001060 vs. ExPASy TrEMBL
Match: A0A6J1IJN1 (gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 265/331 (80.06%), Postives = 293/331 (88.52%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDL KYSLLR+T KP  AA+AADIP IDL+DP+AKSH+VKACEE GFFK+V+
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKP--AASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           HRVP+ELMTKLEDE+L FF LSQ+EKDKARPP PLGYGSKNIG NGDKGWIEY LLNANP
Sbjct: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLF+  S S ++A  EYVTAVKKLS EV ELI EGLKIEP+N IS+L+N+EK+DC FRV
Sbjct: 121 LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           N YPPCPE+QAV G++MIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD  AFF
Sbjct: 181 NHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVM+NGRF+SVKHKVVAD + ERVSMIYFGGP LSEKI PL Q+L +GEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           KEF W EYKTAA+KTRLADYRLG FEK+Q S
Sbjct: 301 KEFTWWEYKTAAFKTRLADYRLGAFEKSQKS 329

[Close]

BLAST of CmoCh07G001060 vs. ExPASy TrEMBL
Match: I0J1J0 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 265/331 (80.06%), Postives = 293/331 (88.52%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDL KYSLLR+T KP  AA+AADIP IDL+DP+AKSH+VKACEE GFFK+V+
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKP--AASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           HRVP+ELMTKLEDE+L FF LSQ+EKDKARPP PLGYGSKNIG NGDKGWIEY LLNANP
Sbjct: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLF+  S S ++A  EYVTAVKKLS EV ELI EGLKIEP+N IS+L+N+EK+DC FRV
Sbjct: 121 LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           N YPPCPE+QAV G++MIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD  AFF
Sbjct: 181 NHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVM+NGRF+SVKHKVVAD + ERVSMIYFGGP LSEKI PL Q+L +GEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           KEF W EYKTAA+KTRLADYRLG FEK+Q S
Sbjct: 301 KEFTWWEYKTAAFKTRLADYRLGAFEKSQKS 329

[Close]

BLAST of CmoCh07G001060 vs. NCBI nr
Match: XP_022926667.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 3.7e-190
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP
Sbjct: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF
Sbjct: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF 335
           KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF
Sbjct: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF 334

[Close]

BLAST of CmoCh07G001060 vs. NCBI nr
Match: KAG6594465.1 (Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667.9 bits (1722), Expect = 4.5e-188
Identity = 330/334 (98.80%), Postives = 332/334 (99.40%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLR TCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRETCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEY LLNANP
Sbjct: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLFAPNS+SLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPD+AAFF
Sbjct: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF 335
           KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF
Sbjct: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF 334

[Close]

BLAST of CmoCh07G001060 vs. NCBI nr
Match: KAG7026463.1 (Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 663.7 bits (1711), Expect = 8.4e-187
Identity = 328/331 (99.09%), Postives = 330/331 (99.70%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEY LLNANP
Sbjct: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLFAPNS+SLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPD+AAFF
Sbjct: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFYWREYKTAAYKTRLADYRLGPFEKNQNS
Sbjct: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of CmoCh07G001060 vs. NCBI nr
Match: XP_023518407.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.0 bits (1678), Expect = 5.7e-183
Identity = 323/334 (96.71%), Postives = 326/334 (97.60%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKP    AAADIPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKP----AAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEY LLNANP
Sbjct: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLFAPNS+SLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           NRYPPCPEL+AVGGKS IGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDA AFF
Sbjct: 181 NRYPPCPELEAVGGKSTIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDATAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVADCN ERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADCNNERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSERF 335
           KEFYW+EYKTAAYKTRLADYRLGPFEKNQNSERF
Sbjct: 301 KEFYWKEYKTAAYKTRLADYRLGPFEKNQNSERF 330

[Close]

BLAST of CmoCh07G001060 vs. NCBI nr
Match: XP_023003444.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 637.9 bits (1644), Expect = 5.0e-179
Identity = 319/333 (95.80%), Postives = 323/333 (97.00%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKP  AAAAA+IPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKP--AAAAANIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           H+VPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEY LLNANP
Sbjct: 61  HQVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLF PNS+SLIAAVKEYVTAVKKLSCEVAELIAE LKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFVPNSESLIAAVKEYVTAVKKLSCEVAELIAEELKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240
           NRYPPCPELQAV GKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDG WVSVPPDAAAFF
Sbjct: 181 NRYPPCPELQAVDGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGEWVSVPPDAAAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVAD N ERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADSNNERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNSER 334
           KEFYWREYKTAAYKT+LADYRLGPFEKNQN ER
Sbjct: 301 KEFYWREYKTAAYKTKLADYRLGPFEKNQNLER 331

[Close]

BLAST of CmoCh07G001060 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 382.1 bits (980), Expect = 4.6e-106
Identity = 192/335 (57.31%), Postives = 250/335 (74.63%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVL QP + L  +  L  T KP     +  IP ++LADP+AK+ IVKACEE GFFKVV+
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           H V  ELMT+LE EA+ FF L Q+ K++A PP P GYG+K IGPNGD GWIEY LLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLI--------AAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
             L +P + ++          +V+EY+  +K++S +V E++AE L IEPR+T+SK+L +E
Sbjct: 121 -QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDE 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           KSD C R+N YP   E      K  +GFGEHTDPQIIS+LRSNN+ GLQIC++DG+WV+V
Sbjct: 181 KSDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAV 240

Query: 241 PPDAAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
           PPD ++FF+NVGDALQVM+NGRF+SVKH+V+AD    R+SMIYFGGP LS+KIAPLP ++
Sbjct: 241 PPDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLV 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
            + ++ LYKEF W +YK++AYK++L DYRLG FEK
Sbjct: 301 PEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of CmoCh07G001060 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 345.1 bits (884), Expect = 6.2e-95
Identity = 178/335 (53.13%), Postives = 228/335 (68.06%), Query Frame = 0


Query: 1   MVVLSQPA-LDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MV++ QPA  D   Y  +   CKP        IP IDL D DAK+ IVKACEE GFFKV+
Sbjct: 1   MVIVLQPASFDSNLY--VNPKCKPRPVL----IPVIDLTDSDAKTQIVKACEEFGFFKVI 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNAN 120
           +H V  +L+T+LE EA+ FF+L  + KDKA PP P GYG+K IGPNGD GW+EY LLNAN
Sbjct: 61  NHGVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNAN 120

Query: 121 -------PLPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
                     +F         AV+EY+  +K++S +  E++ E LKIEP+  +S+L+  +
Sbjct: 121 LCLESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVK 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           +SD C R+N Y   PE +    K  IGFGEHTDPQ+ISLLRSN++ GLQIC++DG WV V
Sbjct: 181 ESDSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDV 240

Query: 241 PPDAAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
            PD ++FFV VGD LQVM+NGRF+SVKH+VV +    R+SMIYF GP LSEKIAPL  ++
Sbjct: 241 TPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLV 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
              ++ LY EF W +YK +AYKT+L DYRLG FEK
Sbjct: 301 PKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of CmoCh07G001060 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 341.7 bits (875), Expect = 6.8e-94
Identity = 165/305 (54.10%), Postives = 227/305 (74.43%), Query Frame = 0


Query: 32  IPAIDLADPDAKSHIVKACEEIGFFKVVSHRVPIELMTKLEDEALRFFSLSQAEKDKARP 91
           IP ID++DP++K  +VKACE+ GFFKV++H V  EL++ LE E + FFSL ++EK +   
Sbjct: 18  IPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV-A 77

Query: 92  PYPLGYGSKNIGPNGDKGWIEYFLLNAN----PLPLFAPNSKS---LIAAVKEYVTAVKK 151
            YP GYG+  IG NGD GW+EY L+NAN      PLF    KS      A++EY T+V+K
Sbjct: 78  GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSVRK 137

Query: 152 LSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRVNRYPPCP--ELQAVGGKSMIGFGE 211
           ++ +V E I +GL I+PRNT+SKL++++ +D   R+N YPPCP    +  GGK++IGFGE
Sbjct: 138 MTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIGFGE 197

Query: 212 HTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFFVNVGDALQVMSNGRFRSVKHKV 271
           HTDPQIIS+LRSNN++GLQI L DG+W+SVPPD  +FF NVGD+LQVM+NGRF+SV+H+V
Sbjct: 198 HTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVRHRV 257

Query: 272 VADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLYKEFYWREYKTAAYKTRLADYRL 328
           +A+C   RVSMIYF GP+L+++IAPL  ++ + +E LY+EF W EYK + Y +RL+D RL
Sbjct: 258 LANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSDNRL 317

[Close]

BLAST of CmoCh07G001060 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 275.4 bits (703), Expect = 6.0e-74
Identity = 143/257 (55.64%), Postives = 185/257 (71.98%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVL QP + L  +  L  T KP     +  IP ++LADP+AK+ IVKACEE GFFKVV+
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120
           H V  ELMT+LE EA+ FF L Q+ K++A PP P GYG+K IGPNGD GWIEY LLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 LPLFAPNSKSLI--------AAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
             L +P + ++          +V+EY+  +K++S +V E++AE L IEPR+T+SK+L +E
Sbjct: 121 -QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDE 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           KSD C R+N YP   E      K  +GFGEHTDPQIIS+LRSNN+ GLQIC++DG+WV+V
Sbjct: 181 KSDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAV 240

Query: 241 PPDAAAFFVNVGDALQV 250
           PPD ++FF+NVGDALQV
Sbjct: 241 PPDHSSFFINVGDALQV 253

[Close]

BLAST of CmoCh07G001060 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 251.5 bits (641), Expect = 9.3e-67
Identity = 134/322 (41.61%), Postives = 194/322 (60.25%), Query Frame = 0


Query: 17  LRATCKPAVAAAAADIPAIDLADPD---AKSHIVKACEEIGFFKVVSHRVPIELMTKLED 76
           L+ T K  +++   + P ID +  D       IVKACE  GFFKV++H V  E++ + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 77  EALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANPLPLFAPNSKSL-- 136
           E   FF+  +++K +A P  P GYG KNIG NGD G +EY LL+ANP  + A  S+++  
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAV-ADKSETISH 128

Query: 137 ------IAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRVNRYPP 196
                  +A  +Y+  V+ L+CE+ +L  E L  +  + +S+L+ + +SD   R+N YPP
Sbjct: 129 DDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPP 188

Query: 197 CPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFFVNVGD 256
            P   A+ G   IGFGEH+DPQI+++LRSN+  GL+IC RDG W+ +P D   FFV VGD
Sbjct: 189 AP--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGD 248

Query: 257 ALQVMSNGRFRSVKHKVVAD-CNIERVSMIYFGGPALSEKIAPLPQILGDGEESLYKEFY 316
            LQ ++NGRF SV+H+V+A+     R+S +YF  P L  KI+PLP+++       Y  F 
Sbjct: 249 CLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFT 308

Query: 317 WREYKTAAYKTRLADYRLGPFE 327
           W +YK A Y  RL   RL  F+
Sbjct: 309 WGDYKKATYSLRLDVPRLEFFK 327

The following BLAST results are available for this feature:

BLAST of CmoCh07G001060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	5.8e-114	61.90	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9XFR9	6.4e-105	57.31	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q9SQ80	8.7e-102	54.85	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
O64692	8.7e-94	53.13	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
Q8S0S6	8.7e-94	54.52	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of CmoCh07G001060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EF07	1.8e-190	100.00	gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC11143372...	[more]
A0A6J1KTC6	2.4e-179	95.80	gibberellin 2-beta-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497054 ...	[more]
A0A2H4V3T7	1.2e-157	83.78	Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S...	[more]
A0A6J1IJN1	1.2e-149	80.06	gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 ...	[more]
I0J1J0	1.2e-149	80.06	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1	[more]

BLAST of CmoCh07G001060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926667.1	3.7e-190	100.00	gibberellin 2-beta-dioxygenase-like [Cucurbita moschata]	[more]
KAG6594465.1	4.5e-188	98.80	Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7026463.1	8.4e-187	99.09	Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_023518407.1	5.7e-183	96.71	gibberellin 2-beta-dioxygenase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023003444.1	5.0e-179	95.80	gibberellin 2-beta-dioxygenase-like [Cucurbita maxima]	[more]

BLAST of CmoCh07G001060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	4.6e-106	57.31	gibberellin 2-oxidase	[more]
AT2G34555.1	6.2e-95	53.13	gibberellin 2-oxidase 3	[more]
AT1G78440.1	6.8e-94	54.10	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G30040.2	6.0e-74	55.64	gibberellin 2-oxidase	[more]
AT1G02400.1	9.3e-67	41.61	gibberellin 2-oxidase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 135..155
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 10..329
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 10..329
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 27..319
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 175..277 e-value: 1.2E-24 score: 86.7
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 32..119 e-value: 3.0E-13 score: 50.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 12..319 e-value: 4.3E-75 score: 255.0
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 173..279 score: 11.627127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh07G001060.1	CmoCh07G001060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding