CmoCh06G015840 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh06G015840 vs. ExPASy Swiss-Prot
Match: Q9C5W0 (Diacylglycerol O-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=DGAT3 PE=1 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 3.8e-36
Identity = 137/344 (39.83%), Postives = 180/344 (52.33%), Query Frame = 0

Query: 24  MEVSGHDARRAPCSLSISGGSVRT------ETRDFGLYVRR---NINSPRFSDDGHVRYY 83
           MEVSG   R+ PC  S S   +R        TR  G   RR    + +  F+D GHV YY
Sbjct: 1   MEVSGVVLRQIPCVSSGSVAGLRLVSEFSGNTRTVGFRTRRFRGIVCNNEFADKGHVNYY 60

Query: 84  V-GTRCHGGR-----LKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDSSVGEFQAD 143
           +  TRC   +     ++KEK+A+KKK K+LK LS +     + GF    +   VGE Q  
Sbjct: 61  IEPTRCGEEKEKVKVMEKEKKALKKKAKVLKSLSKNLDMFSSIGFGLDPEAGLVGEIQTK 120

Query: 144 RFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHD----SSSSSSESSA 203
             +I E  E+L +QL+QLKAEEK  K++RK EKAK KA +  T  D    SSS SS+S  
Sbjct: 121 --TISEATEILVKQLEQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSESSDSDC 180

Query: 204 IEGNMTNTSYRTRKAN------SPSLSQPFSDQWQTNAIQ----GSALAPPLQTHQLNSN 263
            +G + + S    KA        P  +     + Q +AI       AL   LQT  +  +
Sbjct: 181 DKGKVVDMSSLRNKAKPVLEPLQPEATVATLPRIQEDAISCKNTSEALQIALQTSTIFPS 240

Query: 264 TGNFG-----VARSSSVG----RVEVCMGNKCKKAGAAALMEEFERVM-GADSVVCGCKC 323
             N G     V   S VG    RVEVCMG KCK++G A L++EF+R M G +     CKC
Sbjct: 241 MANPGQTLKTVEAVSVVGLPLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSAVACKC 300

BLAST of CmoCh06G015840 vs. ExPASy TrEMBL
Match: A0A6J1GBG4 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111452646 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.1e-185
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60
           MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120
           NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180
           SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240
           ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV
Sbjct: 181 ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300
           ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL
Sbjct: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

Query: 301 DMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPRFAPSI 343
           DMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPRFAPSI
Sbjct: 301 DMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPRFAPSI 342

BLAST of CmoCh06G015840 vs. ExPASy TrEMBL
Match: A0A6J1I582 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC111470783 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.2e-170
Identity = 321/342 (93.86%), Postives = 327/342 (95.61%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60
           MEVSGAVSRRLPGLIRREIAQSS+EVSGHDARRAPCSLSISGGSV TETRDFGL+VRRNI
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSVEVSGHDARRAPCSLSISGGSVGTETRDFGLHVRRNI 60

Query: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120
           NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGL GDSSAA AFGFVFRFDDS
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLPGDSSAALAFGFVFRFDDS 120

Query: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180
           SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARL TA+DSSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLPTAYDSSSSSS 180

Query: 181 ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240
           ESSAIEGNMTNT YRTRKA+SP+LSQ FSDQWQ NAIQGSALAPPLQTHQLNSN GNFGV
Sbjct: 181 ESSAIEGNMTNTRYRTRKASSPTLSQLFSDQWQANAIQGSALAPPLQTHQLNSNIGNFGV 240

Query: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300
           ARS+SVGRVEVCMGNKCKKAGAAALMEEF RVMGADSVVCGCKCMGRCRDGPNVRVS SL
Sbjct: 241 ARSASVGRVEVCMGNKCKKAGAAALMEEFGRVMGADSVVCGCKCMGRCRDGPNVRVSGSL 300

Query: 301 DMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPRFAPSI 343
           DMET SANPLCIGVGVEDVGRIV D LGGQGE KQ RFAPSI
Sbjct: 301 DMETGSANPLCIGVGVEDVGRIVADRLGGQGEQKQSRFAPSI 342

BLAST of CmoCh06G015840 vs. ExPASy TrEMBL
Match: A0A6J1GV38 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111457454 PE=4 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 4.3e-123
Identity = 251/338 (74.26%), Postives = 277/338 (81.95%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60
           MEVSGAV+RRL  L R EIAQ S++V+  DARRAPCSLS+SGGSV T T   GL  RRNI
Sbjct: 1   MEVSGAVTRRLSSLFRCEIAQ-SVDVASADARRAPCSLSVSGGSVSTGTHVIGLVGRRNI 60

Query: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120
           +S RFSD+GH+RYYVG  C G RLKKEKEAVKKKLKLLKGLSG SS     G VFRFDD 
Sbjct: 61  DSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDC 120

Query: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHD-SSSSS 180
            +G+FQADRFSIEETRE L +QLQQLK+EEK+QKR+RKLEKAKLKA R++T H+ SSSSS
Sbjct: 121 LIGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRVKTIHESSSSSS 180

Query: 181 SESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFG 240
           SESS  EG MTNTSY+ RKA    LSQPF DQW+TN I+ S L PPLQ+  LNS+T N G
Sbjct: 181 SESSDNEGYMTNTSYQIRKA----LSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIG 240

Query: 241 VARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSS 300
           VARS SVGRVEVCMGNKCKKAGAAALMEEFERVMGAD  VCGCKCMG+CRDGPNVRVS +
Sbjct: 241 VARSVSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGA 300

Query: 301 LDMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPR 338
           L  ++ SANPLCIGVGVEDVGRIV DHLG QGE KQ R
Sbjct: 301 LAGQSCSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 333

BLAST of CmoCh06G015840 vs. ExPASy TrEMBL
Match: A0A6J1IRZ4 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC111479426 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.9e-118
Identity = 245/338 (72.49%), Postives = 268/338 (79.29%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60
           MEVSGAV+RRL  L R EI Q S++V+  DARRAPCSLS+SGGSV T TR  GL  RRN+
Sbjct: 1   MEVSGAVTRRLSSLFRCEIVQ-SVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNV 60

Query: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120
           +S RFSD+GH+RYYVG  C G RLKKEKEAVKKK KLLKGLSG SS     G VFR DD 
Sbjct: 61  DSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKFKLLKGLSGYSSTDHESGLVFRLDDC 120

Query: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAH-DSSSSS 180
            +G+ QADRFSIEETRE L RQLQQLK+EEK+QKRKRKLEKAKLKA R +T H  SSSSS
Sbjct: 121 LIGDSQADRFSIEETREALIRQLQQLKSEEKDQKRKRKLEKAKLKATRAKTIHKSSSSSS 180

Query: 181 SESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFG 240
           SESS  EG M NT YR RKA    LSQPF DQW+TN I+ S L PPLQ+  LNS+T N G
Sbjct: 181 SESSDNEGYMINTRYRIRKA----LSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIG 240

Query: 241 VARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSS 300
           VA+S SVGRVEVCMGNKCKKAGAAALMEEFERVMGAD  VCGCKCMG+CRDGPNVRVS +
Sbjct: 241 VAQSVSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGA 300

Query: 301 LDMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPR 338
           L  ++ SANPLCIGVGV DVGRIV DHLG QG  KQ R
Sbjct: 301 LARQSCSANPLCIGVGVGDVGRIVADHLGQQGGLKQSR 333

BLAST of CmoCh06G015840 vs. ExPASy TrEMBL
Match: E5GC43 (Diacylglycerol acyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.1e-110
Identity = 230/341 (67.45%), Postives = 263/341 (77.13%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60
           MEV GAVSR+LPGL RR+ +Q S+EV   D RR PCSLSISG SV  ETR  GL  RR +
Sbjct: 1   MEVYGAVSRQLPGLFRRDFSQ-SVEVYDADTRRGPCSLSISGRSVGNETRALGLVSRRRM 60

Query: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120
           +S RF DDG++RYYVG  C GG LKKEKEAVKKKLKLLKGL   S+  +    +FRFD  
Sbjct: 61  DSSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGL---SATDYESSLLFRFDHG 120

Query: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180
           S+ EFQ+DRFSIE+TRE L RQLQQLK EEKEQKRK+KLEKAKLKAAR +T HDSSSSSS
Sbjct: 121 SIDEFQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHDSSSSSS 180

Query: 181 ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240
           ESS  EG+M   SY+ +KA    LSQPF DQ++TNAI  S L  PLQT  LNS T N GV
Sbjct: 181 ESSDNEGHMNTRSYQLKKA----LSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGV 240

Query: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300
            RS+SVGR+EVCMGNKCKKAGAAALMEEFERVMG ++ VCGCKCMG+CRDGPNVRV  S+
Sbjct: 241 TRSTSVGRIEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSM 300

Query: 301 DMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPRFAPS 342
           +M+    NPLCIGVGVEDV RIV ++LG +G   Q R AP+
Sbjct: 301 EMQ----NPLCIGVGVEDVDRIVAEYLGQKGGQTQSRLAPA 329

BLAST of CmoCh06G015840 vs. TAIR 10
Match: AT1G48300.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; Has 96 Blast hits to 87 proteins in 37 species: Archae - 0; Bacteria - 2; Metazoa - 12; Fungi - 2; Plants - 65; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 130.6 bits (327), Expect = 2.5e-30
Identity = 111/269 (41.26%), Postives = 147/269 (54.65%), Query Frame = 0

Query: 84  LKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDSSVGEFQADRFSIEETREVLERQL 143
           ++KEK+A+KKK K+LK LS +     + GF    +   VGE Q    +I E  E+L +QL
Sbjct: 1   MEKEKKALKKKAKVLKSLSKNLDMFSSIGFGLDPEAGLVGEIQTK--TISEATEILVKQL 60

Query: 144 QQLKAEEKEQKRKRKLEKAKLKAARLQTAHD----SSSSSSESSAIEGNMTNTSYRTRKA 203
           +QLKAEEK  K++RK EKAK KA +  T  D    SSS SS+S   +G + + S    KA
Sbjct: 61  EQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSESSDSDCDKGKVVDMSSLRNKA 120

Query: 204 N------SPSLSQPFSDQWQTNAIQ----GSALAPPLQTHQLNSNTGNFG-----VARSS 263
                   P  +     + Q +AI       AL   LQT  +  +  N G     V   S
Sbjct: 121 KPVLEPLQPEATVATLPRIQEDAISCKNTSEALQIALQTSTIFPSMANPGQTLKTVEAVS 180

Query: 264 SVG----RVEVCMGNKCKKAGAAALMEEFERVM-GADSVVCGCKCMGRCRDGPNVRVSSS 323
            VG    RVEVCMG KCK++G A L++EF+R M G +     CKCMG+CRDGPNVRV   
Sbjct: 181 VVGLPLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSAVACKCMGKCRDGPNVRVVKE 240