CmoCh06G015430 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh06G015430
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pectin lyase-like superfamily protein
Location	Cmo_Chr06: 10884313 .. 10886541 (+)
RNA-Seq Expression	CmoCh06G015430
Synteny	CmoCh06G015430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGTTGATTTTCTCGCAGGTTTTGATTTCCAGATTTGATGTTCTTCTGAATTCCTCTTTGATCTGTTCTTCATCTTGTCGGATTAGTTCTTTTCTTGCGATTTTGGGCGATTTGTTATCTGGGTTTCCACCATTGTTGTCTTTTTAAGCTTCTTTTGTTTCATTCTTTAGGTGGCATTGCTGCTGTTGCTACAATTGAGCAATGCTGCGAAGATTATTGGTGATGAGCTCTCCGGACAGTGCAATTCTAAGCCCATATTAGATCCAAGGCCACACAGTGTGTCTATCTTGGAGTTTGGGGCTGTTGGTGATGGCAAAACTCTCAACACCATTGCCTTCCAAAACGCCATTTTCTATCTCAAATCCTTCGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCGGGAAAATGGCTCACTGGAAGCATTAATCTCACTAGCCACCTTACCCTTTTTCTGGAAAAAGGTGCTACCATTCTTGCCTCTCAGGTATGTTCTTATTCTTGGATGAACATAGTGTTTGATTTTAGGCATTTGTTTTGTGATTATCGATCGTATCTTAAACACAGGACCCGTCCCATTGGGAACTTGTCAATCCGTTACCTTCGTATGGTAGAGGTGTTGAAGTTCCAGGGAAACGATACCGGAGCTTGATAAATGGTTACAATTTACACGATGTTGTAATAACAGGTTTGTTATTTGTTCGACACGCTTGATTTAGAATCTAGTTTGTGCGTATGCGATTCACTCGTATGAACATGTGTTATTGTCTTGATTTTCGATGTGCTAGGTGATGATGGTGTCATTGATGGTCAGGGTTTGGTTTGGTGGGATTGGTTCACTTCTCATTCCTTAAACTATAGCCGTCCTCACCTCGTGGAATTTGAGGATTCGGAATATGTAGTCGTTTCGAACCTTAGTTTCTTGAATACTCCTGCTTATAATATCCATCCAGTTTACTGCAGGTATGTCGTAACGTAGCTACTAGAATTCAGTTTCTTCTGCCTTTGTTTGTTCGGTTCACGTTGTTCGTATTTGTGAGCGAATCTGTATTGAAACATAAGTGTTTCATGTGCAGTAATGTCTATGTTTTCAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACTGTTGGAATAGTCCCAGGTTTGAAATGCTTTCTCAGCTTCCTCATTGTCATTTGTTCGACTATTAGTGTGGGCTCCTCTGGCCGAATGGCTCGAGCACCATTGAAGAATATCTCCTTTAATCGATCTCTCGCTCTCTCTCTGTCTCTCACGCACACACACCACGTAGTGCTTAGTAGAAGATGCCATCGAGAAAGATATCAAGGGCAGTTTGCTGAATGTCGTTAGCTCGTTTAAGGAAAACAAGTTCTTATGATTCGGGTTTGAGTCTGGAAAAGATGACTCGTTAGACAGCATCATGATTAGAATGATTTATTAGAATGATGATCAAACCCGGACTTAAAATCTAGAAGTACGATGCGATTCATTCATTTTTGCTTGTAATTAATAAGGCGACGACGCCTTTTCTAAATAAAATTTGATTGGTGCTTTCAGATTCTTCTGATCATGTATGCATAGAGGGCTGCAACATTGCCACGGGATATGACGCCATCGTCTTGAAGAGCGGCTGGGATCAGTATGGTATAGCCTATGGTAGACCATCTAAAAACATACACATCAGAAACGTCCACCTTCAGTCGTCTTCAGGTTCGTCCATTGCCTTTGGTAGCGAGATGTCTGGTGGGATATCTAATGTTCTTGTCGAGCACGTGCACTTGTACAACTCGTCTACTGGCATTGAATTCAGAACTACCAAGGGAAGAGGAGGTTACATTAAAGGAATAGTTATATCAGACGTTGAAATGGAAAACATATCCACGGGATTCAGTGCCTCGGGTCATTTCGGGTCACATCCAGACGACGAGTTCGATCCTAACGCACTTCCAATCGTGCAGGACATAACGTTGCAGAATGTTCGAGGCACAAACATTAAAATTGCTGGAAACTTCACTGGGCTACAGGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATGAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGGTACTCGGAATCCGTTATCCCGGCGCCCTGTTCTGACCTCGATACTCGATATTCAATTTCTTCATTAGCAGCGAATTCAGCTGGAAAAGCTGCTGTTTTATGA

mRNA sequence

ATGATGTTGATTTTCTCGCAGGTGGCATTGCTGCTGTTGCTACAATTGAGCAATGCTGCGAAGATTATTGGTGATGAGCTCTCCGGACAGTGCAATTCTAAGCCCATATTAGATCCAAGGCCACACAGTGTGTCTATCTTGGAGTTTGGGGCTGTTGGTGATGGCAAAACTCTCAACACCATTGCCTTCCAAAACGCCATTTTCTATCTCAAATCCTTCGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCGGGAAAATGGCTCACTGGAAGCATTAATCTCACTAGCCACCTTACCCTTTTTCTGGAAAAAGGTGCTACCATTCTTGCCTCTCAGGACCCGTCCCATTGGGAACTTGTCAATCCGTTACCTTCGTATGGTAGAGGTGTTGAAGTTCCAGGGAAACGATACCGGAGCTTGATAAATGGTTACAATTTACACGATGTTGTAATAACAGGTGATGATGGTGTCATTGATGGTCAGGGTTTGGTTTGGTGGGATTGGTTCACTTCTCATTCCTTAAACTATAGCCGTCCTCACCTCGTGGAATTTGAGGATTCGGAATATGTAGTCGTTTCGAACCTTAGTTTCTTGAATACTCCTGCTTATAATATCCATCCAGTTTACTGCAGTAATGTCTATGTTTTCAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACTGTTGGAATAGTCCCAGATTCTTCTGATCATGTATGCATAGAGGGCTGCAACATTGCCACGGGATATGACGCCATCGTCTTGAAGAGCGGCTGGGATCAGTATGGTATAGCCTATGGTAGACCATCTAAAAACATACACATCAGAAACGTCCACCTTCAGTCGTCTTCAGGTTCGTCCATTGCCTTTGGTAGCGAGATGTCTGGTGGGATATCTAATGTTCTTGTCGAGCACGTGCACTTGTACAACTCGTCTACTGGCATTGAATTCAGAACTACCAAGGGAAGAGGAGGTTACATTAAAGGAATAGTTATATCAGACGTTGAAATGGAAAACATATCCACGGGATTCAGTGCCTCGGGTCATTTCGGGTCACATCCAGACGACGAGTTCGATCCTAACGCACTTCCAATCGTGCAGGACATAACGTTGCAGAATGTTCGAGGCACAAACATTAAAATTGCTGGAAACTTCACTGGGCTACAGGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATGAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGGTACTCGGAATCCGTTATCCCGGCGCCCTGTTCTGACCTCGATACTCGATATTCAATTTCTTCATTAGCAGCGAATTCAGCTGGAAAAGCTGCTGTTTTATGA

Coding sequence (CDS)

ATGATGTTGATTTTCTCGCAGGTGGCATTGCTGCTGTTGCTACAATTGAGCAATGCTGCGAAGATTATTGGTGATGAGCTCTCCGGACAGTGCAATTCTAAGCCCATATTAGATCCAAGGCCACACAGTGTGTCTATCTTGGAGTTTGGGGCTGTTGGTGATGGCAAAACTCTCAACACCATTGCCTTCCAAAACGCCATTTTCTATCTCAAATCCTTCGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCGGGAAAATGGCTCACTGGAAGCATTAATCTCACTAGCCACCTTACCCTTTTTCTGGAAAAAGGTGCTACCATTCTTGCCTCTCAGGACCCGTCCCATTGGGAACTTGTCAATCCGTTACCTTCGTATGGTAGAGGTGTTGAAGTTCCAGGGAAACGATACCGGAGCTTGATAAATGGTTACAATTTACACGATGTTGTAATAACAGGTGATGATGGTGTCATTGATGGTCAGGGTTTGGTTTGGTGGGATTGGTTCACTTCTCATTCCTTAAACTATAGCCGTCCTCACCTCGTGGAATTTGAGGATTCGGAATATGTAGTCGTTTCGAACCTTAGTTTCTTGAATACTCCTGCTTATAATATCCATCCAGTTTACTGCAGTAATGTCTATGTTTTCAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACTGTTGGAATAGTCCCAGATTCTTCTGATCATGTATGCATAGAGGGCTGCAACATTGCCACGGGATATGACGCCATCGTCTTGAAGAGCGGCTGGGATCAGTATGGTATAGCCTATGGTAGACCATCTAAAAACATACACATCAGAAACGTCCACCTTCAGTCGTCTTCAGGTTCGTCCATTGCCTTTGGTAGCGAGATGTCTGGTGGGATATCTAATGTTCTTGTCGAGCACGTGCACTTGTACAACTCGTCTACTGGCATTGAATTCAGAACTACCAAGGGAAGAGGAGGTTACATTAAAGGAATAGTTATATCAGACGTTGAAATGGAAAACATATCCACGGGATTCAGTGCCTCGGGTCATTTCGGGTCACATCCAGACGACGAGTTCGATCCTAACGCACTTCCAATCGTGCAGGACATAACGTTGCAGAATGTTCGAGGCACAAACATTAAAATTGCTGGAAACTTCACTGGGCTACAGGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATGAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGGTACTCGGAATCCGTTATCCCGGCGCCCTGTTCTGACCTCGATACTCGATATTCAATTTCTTCATTAGCAGCGAATTCAGCTGGAAAAGCTGCTGTTTTATGA

Protein sequence

MMLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVSGYSESVIPAPCSDLDTRYSISSLAANSAGKAAVL

Homology

[Close]

BLAST of CmoCh06G015430 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.8e-111
Identity = 202/437 (46.22%), Postives = 275/437 (62.93%), Query Frame = 0


Query: 8   VALLLLLQLSNAAKIIGDELSGQCNSKPILDP--------RPHSVSILEFGAVGDGKTLN 67
           VA++LLL +S           G+C    IL+         R HS S+++FG VGDG+TLN
Sbjct: 29  VAVVLLLSVSR----------GECRKGRILEALEYSAISCRAHSASLVDFGGVGDGQTLN 88

Query: 68  TIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWE 127
           T AFQ+A+  L  +  +GGAQLYVP GKWLTGS +LTSH TLFL + A +LASQD S W 
Sbjct: 89  TKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWP 148

Query: 128 LVNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSR 187
           ++ PLPSYGRG +    RY SLI G NL DV+ITGD+G IDGQG +WW  F    L Y+R
Sbjct: 149 VIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTR 208

Query: 188 PHLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSD 247
           P+L+E   S  + +SNL+ LN+P++N+HPVY  N+ +  I++ AP  SP T GI PDS  
Sbjct: 209 PYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCT 268

Query: 248 HVCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGG 307
           +  IE C I +G D + +KSGWD+YGIAYG P+K + IR +   S   + IA GSEMSGG
Sbjct: 269 NTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGG 328

Query: 308 ISNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDE 367
           I +V  E +   NS +GI  +T  GRGGY+K I +  + M+ +   F  +G++GSH D+ 
Sbjct: 329 IQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNH 388

Query: 368 FDPNALPIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNS-SSSTSWICS 427
           +DP A P++Q I  +++   N+ +A    G+   PFT I +SN+T  + + +    W C+
Sbjct: 389 YDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCT 448

Query: 428 DVSGYSESVIPAPCSDL 436
           DV G S  V P PCS L
Sbjct: 449 DVEGISSGVTPTPCSTL 455

[Close]

BLAST of CmoCh06G015430 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 1.1e-25
Identity = 105/385 (27.27%), Postives = 178/385 (46.23%), Query Frame = 0


Query: 41  PHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGK-WLTGSINLTS-- 100
           P +VS+ +FGA GDGKT +T AF NA  + K+ +  G   L VP G  +L  SI LT   
Sbjct: 65  PTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGPC 124

Query: 101 HLTLFLEKGATILASQDPSHWELVNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDG 160
           +  L ++   T+ ASQ  S ++ ++                  + +G N +  V  GD G
Sbjct: 125 NSILTVQIFGTLSASQKRSDYKDISKW---------------IMFDGVN-NLSVDGGDTG 184

Query: 161 VIDGQGLVWWDWFTSHSLNYSR-----PHLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCS 220
           V+DG G  WW    S   N ++     P  + F +S+ ++V NL   N     I    CS
Sbjct: 185 VVDGNGETWWQ--NSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCS 244

Query: 221 NVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIVLKSGWDQYGIAYGRPS 280
           NV V N+ V+AP++SP T GI   ++ ++ +    I TG D I ++SG           S
Sbjct: 245 NVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------S 304

Query: 281 KNIHIRNVHLQSSSGSSI-AFGSEMSGG-ISNVLVEHVHLYNSSTGIEFRTTKGRGGYIK 340
           +N+ I ++      G SI + G + S   +S V V+   L  +  G+  +T +G  G   
Sbjct: 305 QNVQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTAS 364

Query: 341 GIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDITLQNVRGTNI-KIAGNFTG 400
            I+  +++M+N+         +        + +A+  V+++  +++ GT+  + A  F  
Sbjct: 365 NIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDISGTSASENAITFNC 417

Query: 401 LQESPFTSIYLSNITFSMNSSSSTS 415
            +  P   I L  +      ++ T+
Sbjct: 425 SKNYPCQGIVLDRVNIKGGKATCTN 417

[Close]

BLAST of CmoCh06G015430 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.4e-24
Identity = 109/407 (26.78%), Postives = 179/407 (43.98%), Query Frame = 0


Query: 42  HSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTL 101
           +++ + +FGAVGDG T ++ AF  A  +    +  G  Q  VP G        +T  L  
Sbjct: 22  NALDVTQFGAVGDGVTDDSQAFLKA--WEAVCSGTGDGQFVVPAG--------MTFMLQP 81

Query: 102 FLEKGATILASQDPSHWELVNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDG 161
              +G+       P   +++  L +  +G     K    L    ++  +VI G DG I+G
Sbjct: 82  LKFQGS---CKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFT--DIEGLVIEG-DGEING 141

Query: 162 QGLVWWDWFTSHSLNYSRPHLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISV 221
           QG  WW+         SRP  ++F     + +S L+ L++P  +IH   C+ V + ++ +
Sbjct: 142 QGSSWWEH------KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRI 201

Query: 222 SAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVH 281
           +AP  SP T GI   +S +V I+ C IATG D I + SG           + NIHI  + 
Sbjct: 202 NAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNIHISGI- 261

Query: 282 LQSSSGSSIAFGSEMSGG----ISNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDV 341
                G  I+ GS    G    + NV V++ +   +  G   +T +G  GY + I  + +
Sbjct: 262 -DCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGI 321

Query: 342 EMENISTGFSASGHF-GSHPDDEFDPNALPI-VQDITLQNVRGTNIKIAG-NFTGLQESP 401
            ++N+         + G   D+  D  +  + V  +   N  GT+    G +F   +  P
Sbjct: 322 TLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVP 381

Query: 402 FTSIYLSNI---TFSMNSSSSTSWICSDVSGYSESVIPA-PCSDLDT 438
            T I+L ++   T S  S       C +V G S   +P   C +L T
Sbjct: 382 CTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLELST 393

[Close]

BLAST of CmoCh06G015430 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.4e-22
Identity = 116/457 (25.38%), Postives = 195/457 (42.67%), Query Frame = 0


Query: 1   MMLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHS-------VSILEFGAVG 60
           M  +  Q+ ++   +  +A  ++  ++     S   L    HS        ++ ++GAVG
Sbjct: 9   MAFVAMQLIIMAAAEDQSAQIMLDSDIEQYLRSNRSLRKVEHSRHDAINIFNVEKYGAVG 68

Query: 61  DGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPG-KWLTGSINLTS----HLTLFLEKGAT 120
           DGK   T AF  A    ++   K  A L VP   K++  ++        H T  ++    
Sbjct: 69  DGKHDCTEAFSTA---WQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFTFKVD--GI 128

Query: 121 ILASQDPSHWELVNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWW- 180
           I A Q+P+ W                 K  R  +    L    + G  GVIDGQG  WW 
Sbjct: 129 IAAYQNPASW-----------------KNNRIWLQFAKLTGFTLMG-KGVIDGQGKQWWA 188

Query: 181 ---DWFTSHSL--NYSRPHLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVS 240
               W     +  +  RP  ++F+ S  +++  L  +N+P +++    C  V +  IS++
Sbjct: 189 GQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISIT 248

Query: 241 APSESPYTVGIVPDSSDHVCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHL 300
           AP +SP T GI   +S +  ++   I TG D + + +G           S NI I ++  
Sbjct: 249 APRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTG-----------SSNIVIEDLIC 308

Query: 301 QSSSGSSI-AFGSEMS-GGISNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEME 360
               G SI + G E S   +S V V      ++  G+  +T +G  G    I+  +VEM 
Sbjct: 309 GPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMI 368

Query: 361 NISTGFSASGHFGSHPDDEFDPNALPIVQDITLQNVRGTNIKIAGNFTGLQES-PFTSIY 420
           N       +  + +      +  +   +QD+T +N+RGT+   A       +S P   I 
Sbjct: 369 NSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIK 428

Query: 421 LSNITFSMNSSSSTSWICSDVSGY-SESVIPAPCSDL 436
           LS+I+  + S    S +  + +GY S  VIPA C +L
Sbjct: 429 LSDISLKLTSGKIASCLNDNANGYFSGHVIPA-CKNL 430

[Close]

BLAST of CmoCh06G015430 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 7.9e-21
Identity = 99/386 (25.65%), Postives = 166/386 (43.01%), Query Frame = 0


Query: 44  VSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGK-WLTGSINLTS--HLT 103
           +S+ +FGA GDG T +T AF+ A       A   G+ L VP  K +L   I  +      
Sbjct: 85  ISVDDFGARGDG-TDDTKAFEKA----WKDACSSGSVLIVPENKNYLLKQITFSGPCKSD 144

Query: 104 LFLEKGATILASQDPSHWELVNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVID 163
           L ++   TI AS D S W                G   +  I   ++ ++ + G  G  +
Sbjct: 145 LRVKIRGTIEASSDQSDW---------------VGHNRKRWIEFEDISNLTLEG-GGTSN 204

Query: 164 GQGLVWWDWFTSHSLNY---SRPHLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVF 223
           G G  WWD       +    S P  + F   + ++VS+LS  ++   ++    C +V   
Sbjct: 205 GNGETWWDSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIAS 264

Query: 224 NISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHI 283
           N+ V+AP  SP T GI    +  + +    I TG D I ++SG           SK +  
Sbjct: 265 NLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESG-----------SKMVIA 324

Query: 284 RNVHLQSSSGSSI-AFGSEMS-GGISNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVIS 343
            N+      G SI + G   S   +S VLV+  +L++++ G+  +T +G  G  K I   
Sbjct: 325 TNITCGPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQ 384

Query: 344 DVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDITLQNVRGTNI-KIAGNFTGLQESP 403
           ++ M N++       ++    D   +  +   V ++   N+RGT+  ++A  F   + SP
Sbjct: 385 NIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSP 438

Query: 404 FTSIYLSNITFSMNSSSSTSWICSDV 421
                + NI    N    T+  CS++
Sbjct: 445 CQGYIVGNINLVGNGGKETTMSCSNI 438

[Close]

BLAST of CmoCh06G015430 vs. ExPASy TrEMBL
Match: A0A6J1FGQ0 (probable polygalacturonase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445517 PE=3 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.1e-263
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0


Query: 1   MMLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNT 60
           MMLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNT
Sbjct: 1   MMLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNT 60

Query: 61  IAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWEL 120
           IAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWEL
Sbjct: 61  IAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWEL 120

Query: 121 VNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRP 180
           VNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRP
Sbjct: 121 VNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRP 180

Query: 181 HLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDH 240
           HLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDH
Sbjct: 181 HLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDH 240

Query: 241 VCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGI 300
           VCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGI
Sbjct: 241 VCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGI 300

Query: 301 SNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEF 360
           SNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEF
Sbjct: 301 SNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEF 360

Query: 361 DPNALPIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDV 420
           DPNALPIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDV
Sbjct: 361 DPNALPIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDV 420

Query: 421 SGYSESVIPAPCSDLDTRYSISSLAANSAGKAAVL 456
           SGYSESVIPAPCSDLDTRYSISSLAANSAGKAAVL
Sbjct: 421 SGYSESVIPAPCSDLDTRYSISSLAANSAGKAAVL 455

[Close]

BLAST of CmoCh06G015430 vs. ExPASy TrEMBL
Match: A0A6J1FI43 (probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445517 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 3.8e-260
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0


Query: 8   VALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 67
           VALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 5   VALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 64

Query: 68  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLPSY 127
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLPSY
Sbjct: 65  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLPSY 124

Query: 128 GRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFED 187
           GRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFED
Sbjct: 125 GRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFED 184

Query: 188 SEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCN 247
           SEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 185 SEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCN 244

Query: 248 IATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEH 307
           IATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 245 IATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEH 304

Query: 308 VHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 367
           VHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI
Sbjct: 305 VHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 364

Query: 368 VQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVSGYSESV 427
           VQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVSGYSESV
Sbjct: 365 VQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVSGYSESV 424

Query: 428 IPAPCSDLDTRYSISSLAANSAGKAAVL 456
           IPAPCSDLDTRYSISSLAANSAGKAAVL
Sbjct: 425 IPAPCSDLDTRYSISSLAANSAGKAAVL 452

[Close]

BLAST of CmoCh06G015430 vs. ExPASy TrEMBL
Match: A0A6J1I844 (probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470858 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.4e-259
Identity = 451/459 (98.26%), Postives = 451/459 (98.26%), Query Frame = 0


Query: 1   MMLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNT 60
           MMLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNT
Sbjct: 1   MMLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNT 60

Query: 61  IAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWEL 120
           IAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATIL SQDPSHWEL
Sbjct: 61  IAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWEL 120

Query: 121 VNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRP 180
           VNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRP
Sbjct: 121 VNPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRP 180

Query: 181 HLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDH 240
           HLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDH
Sbjct: 181 HLVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDH 240

Query: 241 VCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGI 300
           VCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGI
Sbjct: 241 VCIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGI 300

Query: 301 SNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEF 360
           SNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEF
Sbjct: 301 SNVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEF 360

Query: 361 DPNALPIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDV 420
           DPNALPIVQDITLQNVRGTNI IAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWIC DV
Sbjct: 361 DPNALPIVQDITLQNVRGTNINIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICLDV 420

Query: 421 SGYSESVIPAPCSDLDTRYSISSLAA----NSAGKAAVL 456
           SGYSESVIP PCSDLDTRYSISSLAA    NSAGKAAVL
Sbjct: 421 SGYSESVIPPPCSDLDTRYSISSLAATSLLNSAGKAAVL 459

[Close]

BLAST of CmoCh06G015430 vs. ExPASy TrEMBL
Match: A0A6J1I4D7 (probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470858 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 4.3e-256
Identity = 444/452 (98.23%), Postives = 444/452 (98.23%), Query Frame = 0


Query: 8   VALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 67
           VALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 5   VALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 64

Query: 68  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLPSY 127
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATIL SQDPSHWELVNPLPSY
Sbjct: 65  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSY 124

Query: 128 GRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFED 187
           GRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFED
Sbjct: 125 GRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFED 184

Query: 188 SEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCN 247
           SEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 185 SEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCN 244

Query: 248 IATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEH 307
           IATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 245 IATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEH 304

Query: 308 VHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 367
           VHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI
Sbjct: 305 VHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 364

Query: 368 VQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVSGYSESV 427
           VQDITLQNVRGTNI IAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWIC DVSGYSESV
Sbjct: 365 VQDITLQNVRGTNINIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICLDVSGYSESV 424

Query: 428 IPAPCSDLDTRYSISSLAA----NSAGKAAVL 456
           IP PCSDLDTRYSISSLAA    NSAGKAAVL
Sbjct: 425 IPPPCSDLDTRYSISSLAATSLLNSAGKAAVL 456

[Close]

BLAST of CmoCh06G015430 vs. ExPASy TrEMBL
Match: A0A1S3AX49 (probable polygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483871 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.0e-241
Identity = 418/458 (91.27%), Postives = 434/458 (94.76%), Query Frame = 0


Query: 2   MLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTI 61
           MLIF QVALLL LQLSNAA+IIG+EL+GQC+SKP LDPRPHSVSILEFGAVGDGKTLNTI
Sbjct: 1   MLIFWQVALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTI 60

Query: 62  AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELV 121
           AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGA IL SQDPSHWELV
Sbjct: 61  AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELV 120

Query: 122 NPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPH 181
           NPLPSYGRG+EVPGKRYRSLINGYNL DVVITGDDGVIDGQGLVWW+WF+SHSLNYSRPH
Sbjct: 121 NPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPH 180

Query: 182 LVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHV 241
           LVEFEDS+YVVVSNL+FLNTPAYNIHPVYCSNVYV+NISV APSESPYTVGIVPDSSDHV
Sbjct: 181 LVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHV 240

Query: 242 CIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGIS 301
           CIEGCNIATGYDAI LKSGWDQYGIAYGRPSKNIHIR VHLQSSSGSSIAFGSEMSGGIS
Sbjct: 241 CIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGIS 300

Query: 302 NVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFD 361
           NVLVEHV L NS  GIEFRTTKGRGGYIKGIV+SDVEMENIST FSASGHFGSHPDDE+D
Sbjct: 301 NVLVEHVQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYD 360

Query: 362 PNALPIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVS 421
           PNALPIVQDITLQNVRGTNIKIAGNF+G+QESPFTSIYLSNITFS+NSSSSTSWICSDVS
Sbjct: 361 PNALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVS 420

Query: 422 GYSESVIPAPCSDLDTRYSISSLAA----NSAGKAAVL 456
           G+SESVIP PCSDL T YSISS AA    NS GK AVL
Sbjct: 421 GFSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458

[Close]

BLAST of CmoCh06G015430 vs. TAIR 10
Match: AT3G06770.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 578.9 bits (1491), Expect = 3.5e-165
Identity = 286/453 (63.13%), Postives = 354/453 (78.15%), Query Frame = 0


Query: 2   MLIFSQVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTI 61
           +LI +  +++ +    + A  I DEL      +  L  RPHSVSI +FGAVGDGKTLNT+
Sbjct: 6   ILILALTSVIPIYANVHGAGKICDEL-----GRRSLSTRPHSVSITDFGAVGDGKTLNTL 65

Query: 62  AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELV 121
           AFQNA+FYL SFADKGGAQLYVPPG WLTGS +LTSHLTLFLE GA I+ASQDPSHWE+V
Sbjct: 66  AFQNAVFYLMSFADKGGAQLYVPPGHWLTGSFSLTSHLTLFLENGAVIVASQDPSHWEVV 125

Query: 122 NPLPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPH 181
           +PLPSYGRG+++PGKRY+SLING  LHDVV+TGD+G IDGQGLVWWD FTSHSL Y+RPH
Sbjct: 126 DPLPSYGRGIDLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSHSLKYNRPH 185

Query: 182 LVEFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHV 241
           L+EF  SE V+VSNL+FLN PAY+I+ +Y S+VY+  I   +  +SPYT+GIVPDSSD+V
Sbjct: 186 LIEFLSSENVIVSNLTFLNAPAYSIYSIYSSHVYIHKILAHSSPKSPYTIGIVPDSSDYV 245

Query: 242 CIEGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGIS 301
           CI+   I  GYDAI LKSGWD+YGIAY RP++N+HIRNV+L+ +SGSSI+FGSEMSGGIS
Sbjct: 246 CIQNSTINVGYDAISLKSGWDEYGIAYSRPTENVHIRNVYLRGASGSSISFGSEMSGGIS 305

Query: 302 NVLVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFD 361
           +V+V++ H++ S TGI FRTTKGRGGYIK I IS+++M  I T   A+G FGSHPDD++D
Sbjct: 306 DVVVDNAHIHYSLTGIAFRTTKGRGGYIKEIDISNIDMLRIGTAIVANGSFGSHPDDKYD 365

Query: 362 PNALPIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVS 421
            NALP+V  I L N+ G NI IAG   G++ESPF+S+ LSN++ SM+S SS SW CS V 
Sbjct: 366 VNALPLVSHIRLSNISGENIGIAGKLFGIKESPFSSVTLSNVSLSMSSGSSVSWQCSYVY 425

Query: 422 GYSESVIPAPCSDLDTRYSISSLAANSAGKAAV 455
           G SESVIP PC +L          A++ G+AAV
Sbjct: 426 GSSESVIPEPCPEL-------KRDADAYGRAAV 446

[Close]

BLAST of CmoCh06G015430 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 569.7 bits (1467), Expect = 2.1e-162
Identity = 268/428 (62.62%), Postives = 339/428 (79.21%), Query Frame = 0


Query: 8   VALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 67
           VAL+ LL  +    I G+  +  C     LDPRPHSVSILEFGAVGDGKTLNTIAFQNA+
Sbjct: 5   VALVWLLAFT-ILLISGEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNTIAFQNAV 64

Query: 68  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLPSY 127
           FYLKSFADKGGAQLYVPPGKWLTGS NLTSHLTLFLEKGATILAS DPSHW++V+PLPSY
Sbjct: 65  FYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDVVSPLPSY 124

Query: 128 GRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFED 187
           GRG+E+PGKRYRSLING NL DVVITG++G  DGQG  WW+W  S SLNYSRPH++EF  
Sbjct: 125 GRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRPHIIEFVS 184

Query: 188 SEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCN 247
           S+++++SNL+FLN PA NIHPVYCS +++  + +    +SP+ +G+ PDSSD+VCIE   
Sbjct: 185 SKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDNVCIEDST 244

Query: 248 IATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEH 307
           I  G+DA+ LKSGWDQYGI YGRP+  +HIRN+ L+S +G+ I+FGSEMSGG+S+V VE 
Sbjct: 245 INVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGVSDVTVER 304

Query: 308 VHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 367
           +++++S  G+ FRTT+GRGGYI+ I ISDV++ ++ T   A+GH GSHPDD+FD +ALP+
Sbjct: 305 LNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKFDRDALPV 364

Query: 368 VQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVSGYSESV 427
           V  I ++N  G +I +AGN TG+ ESPFTSI L++I   + + S  SWICS+VSG+S+ V
Sbjct: 365 VTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADI--HLQTRSEESWICSNVSGFSDDV 424

Query: 428 IPAPCSDL 436
            P PC +L
Sbjct: 425 SPEPCQEL 429

[Close]

BLAST of CmoCh06G015430 vs. TAIR 10
Match: AT5G49215.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 559.3 bits (1440), Expect = 2.8e-159
Identity = 276/432 (63.89%), Postives = 334/432 (77.31%), Query Frame = 0


Query: 8   VALLLLLQLSNAAKIIGDE--LSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQN 67
           VAL+LLL LS+   I G    +   C+       RPHSV I +FGAVGDGKTLNT+AFQN
Sbjct: 5   VALVLLLTLSSVILINGRSYGVGNICDRGRRPSERPHSVKITDFGAVGDGKTLNTLAFQN 64

Query: 68  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLP 127
           A+FYLKSFADKGGAQLYVPPG+WLTGS NLTSHLTLFLEK A ILASQDPSHW++ + LP
Sbjct: 65  AVFYLKSFADKGGAQLYVPPGRWLTGSFNLTSHLTLFLEKDAVILASQDPSHWQVTDALP 124

Query: 128 SYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEF 187
           SYGRG+++PGKRY SLING  LHDVV+TGD+G IDGQGLVWWD F SHSL YSRPHLVEF
Sbjct: 125 SYGRGIDLPGKRYMSLINGDMLHDVVVTGDNGTIDGQGLVWWDRFNSHSLEYSRPHLVEF 184

Query: 188 EDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEG 247
             +E V+VSNL+FLN PAY IH VYC N+Y+  ++ +   ESPYT+GIVPDSS++VCI+ 
Sbjct: 185 VSAENVIVSNLTFLNAPAYTIHSVYCRNLYIHRVTANTCPESPYTIGIVPDSSENVCIQE 244

Query: 248 CNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLV 307
            +I  GYDAI LKSG D+YG++Y RP+ N+ IRNV+L+++SGSSI+FGSEMSGGIS+V V
Sbjct: 245 SSINMGYDAISLKSGCDEYGLSYARPTANVQIRNVYLRAASGSSISFGSEMSGGISDVEV 304

Query: 308 EHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNAL 367
              H++NS +GI FRTT GRGGYIK I IS++ M N+ T F A+G FG+HPD  FD NA 
Sbjct: 305 SDAHIHNSLSGIAFRTTNGRGGYIKEIDISNIHMVNVGTAFLANGSFGTHPDSGFDENAY 364

Query: 368 PIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSS--TSWICSDVSGY 427
           P+V  I L ++ G NI  AG F G +ESPFTSI LSNI+ S+ +S+S   SW CS V G 
Sbjct: 365 PLVSHIRLHDIVGENISTAGYFFGTKESPFTSILLSNISLSIKNSASPADSWQCSYVDGS 424

Query: 428 SESVIPAPCSDL 436
           SE V+P PC +L
Sbjct: 425 SEFVVPEPCLEL 436

[Close]

BLAST of CmoCh06G015430 vs. TAIR 10
Match: AT5G49215.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 559.3 bits (1440), Expect = 2.8e-159
Identity = 276/432 (63.89%), Postives = 334/432 (77.31%), Query Frame = 0


Query: 8   VALLLLLQLSNAAKIIGDE--LSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQN 67
           VAL+LLL LS+   I G    +   C+       RPHSV I +FGAVGDGKTLNT+AFQN
Sbjct: 3   VALVLLLTLSSVILINGRSYGVGNICDRGRRPSERPHSVKITDFGAVGDGKTLNTLAFQN 62

Query: 68  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLP 127
           A+FYLKSFADKGGAQLYVPPG+WLTGS NLTSHLTLFLEK A ILASQDPSHW++ + LP
Sbjct: 63  AVFYLKSFADKGGAQLYVPPGRWLTGSFNLTSHLTLFLEKDAVILASQDPSHWQVTDALP 122

Query: 128 SYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEF 187
           SYGRG+++PGKRY SLING  LHDVV+TGD+G IDGQGLVWWD F SHSL YSRPHLVEF
Sbjct: 123 SYGRGIDLPGKRYMSLINGDMLHDVVVTGDNGTIDGQGLVWWDRFNSHSLEYSRPHLVEF 182

Query: 188 EDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEG 247
             +E V+VSNL+FLN PAY IH VYC N+Y+  ++ +   ESPYT+GIVPDSS++VCI+ 
Sbjct: 183 VSAENVIVSNLTFLNAPAYTIHSVYCRNLYIHRVTANTCPESPYTIGIVPDSSENVCIQE 242

Query: 248 CNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLV 307
            +I  GYDAI LKSG D+YG++Y RP+ N+ IRNV+L+++SGSSI+FGSEMSGGIS+V V
Sbjct: 243 SSINMGYDAISLKSGCDEYGLSYARPTANVQIRNVYLRAASGSSISFGSEMSGGISDVEV 302

Query: 308 EHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNAL 367
              H++NS +GI FRTT GRGGYIK I IS++ M N+ T F A+G FG+HPD  FD NA 
Sbjct: 303 SDAHIHNSLSGIAFRTTNGRGGYIKEIDISNIHMVNVGTAFLANGSFGTHPDSGFDENAY 362

Query: 368 PIVQDITLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSS--TSWICSDVSGY 427
           P+V  I L ++ G NI  AG F G +ESPFTSI LSNI+ S+ +S+S   SW CS V G 
Sbjct: 363 PLVSHIRLHDIVGENISTAGYFFGTKESPFTSILLSNISLSIKNSASPADSWQCSYVDGS 422

Query: 428 SESVIPAPCSDL 436
           SE V+P PC +L
Sbjct: 423 SEFVVPEPCLEL 434

[Close]

BLAST of CmoCh06G015430 vs. TAIR 10
Match: AT3G06770.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 521.9 bits (1343), Expect = 5.0e-148
Identity = 251/383 (65.54%), Postives = 308/383 (80.42%), Query Frame = 0


Query: 72  SFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILASQDPSHWELVNPLPSYGRGV 131
           SFADKGGAQLYVPPG WLTGS +LTSHLTLFLE GA I+ASQDPSHWE+V+PLPSYGRG+
Sbjct: 2   SFADKGGAQLYVPPGHWLTGSFSLTSHLTLFLENGAVIVASQDPSHWEVVDPLPSYGRGI 61

Query: 132 EVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFEDSEYV 191
           ++PGKRY+SLING  LHDVV+TGD+G IDGQGLVWWD FTSHSL Y+RPHL+EF  SE V
Sbjct: 62  DLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSHSLKYNRPHLIEFLSSENV 121

Query: 192 VVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATG 251
           +VSNL+FLN PAY+I+ +Y S+VY+  I   +  +SPYT+GIVPDSSD+VCI+   I  G
Sbjct: 122 IVSNLTFLNAPAYSIYSIYSSHVYIHKILAHSSPKSPYTIGIVPDSSDYVCIQNSTINVG 181

Query: 252 YDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEHVHLY 311
           YDAI LKSGWD+YGIAY RP++N+HIRNV+L+ +SGSSI+FGSEMSGGIS+V+V++ H++
Sbjct: 182 YDAISLKSGWDEYGIAYSRPTENVHIRNVYLRGASGSSISFGSEMSGGISDVVVDNAHIH 241

Query: 312 NSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDI 371
            S TGI FRTTKGRGGYIK I IS+++M  I T   A+G FGSHPDD++D NALP+V  I
Sbjct: 242 YSLTGIAFRTTKGRGGYIKEIDISNIDMLRIGTAIVANGSFGSHPDDKYDVNALPLVSHI 301

Query: 372 TLQNVRGTNIKIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICSDVSGYSESVIPAP 431
            L N+ G NI IAG   G++ESPF+S+ LSN++ SM+S SS SW CS V G SESVIP P
Sbjct: 302 RLSNISGENIGIAGKLFGIKESPFSSVTLSNVSLSMSSGSSVSWQCSYVYGSSESVIPEP 361

Query: 432 CSDLDTRYSISSLAANSAGKAAV 455
           C +L          A++ G+AAV
Sbjct: 362 CPEL-------KRDADAYGRAAV 377

The following BLAST results are available for this feature:

BLAST of CmoCh06G015430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	2.8e-111	46.22	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q8RY29	1.1e-25	27.27	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
Q9LW07	3.4e-24	26.78	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
P43212	2.4e-22	25.38	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q02096	7.9e-21	25.65	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]

BLAST of CmoCh06G015430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FGQ0	2.1e-263	100.00	probable polygalacturonase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445...	[more]
A0A6J1FI43	3.8e-260	100.00	probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445...	[more]
A0A6J1I844	2.4e-259	98.26	probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147085...	[more]
A0A6J1I4D7	4.3e-256	98.23	probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147085...	[more]
A0A1S3AX49	1.0e-241	91.27	probable polygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483871 PE...	[more]

BLAST of CmoCh06G015430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06770.2	3.5e-165	63.13	Pectin lyase-like superfamily protein	[more]
AT3G16850.1	2.1e-162	62.62	Pectin lyase-like superfamily protein	[more]
AT5G49215.1	2.8e-159	63.89	Pectin lyase-like superfamily protein	[more]
AT5G49215.2	2.8e-159	63.89	Pectin lyase-like superfamily protein	[more]
AT3G06770.3	5.0e-148	65.54	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 300..321 e-value: 330.0 score: 10.1 coord: 363..385 e-value: 4700.0 score: 2.4 coord: 238..259 e-value: 1100.0 score: 6.9 coord: 272..294 e-value: 240.0 score: 10.5 coord: 188..210 e-value: 4100.0 score: 2.8 coord: 329..350 e-value: 2000.0 score: 5.1
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 133..421 e-value: 5.6E-33 score: 114.4
IPR024535	Pectate lyase superfamily protein	PFAM	PF12708	Pectate_lyase_3	coord: 44..101 e-value: 1.2E-7 score: 31.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 30..425 e-value: 2.4E-112 score: 377.9
None	No IPR available	PANTHER	PTHR31339:SF15	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 7..445
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 7..445
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 39..435

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G015430.1	CmoCh06G015430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity