CmoCh06G015190 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G015190
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectinesterase
LocationCmo_Chr06: 10816260 .. 10818029 (-)
RNA-Seq ExpressionCmoCh06G015190
SyntenyCmoCh06G015190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTATGACGGCGGTGGATGCAAGAACAATAAAGCTGCCTTGGTTGGGTTGTTGTCTCTGTTTTTGGTTGCCTTGGTTAATGGTGTAAGTGGCGGAGGAGAGGACTCTGGCTCCTCTAATGCTGCCATTAAGACTCTATGTGAGCCCACTACTTACCGTGAAACTTGCGAAGAAGCCCTGGTGAAGTCTAATGTTGACTCAAAAGACCCACGTGAGTTGGTCAAGGCAGGGTTCCATTTTGCTATTGAGCAGCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAAACAAGCTACCACCGACCCCATGGCGCAAAAAGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTGATAAAATCACCAATAGTTTTAATTTACACAAAATGAATGATTATCTTGAGAATCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAAGAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGATGTCAAAAGAAATGACCGCCAATGGGCTTGTGATGGTGGGTGAGGTTACATCCCTTGTGAGCACTTTGTGGGAGAAGTTGGGACTGCCCCCACAAACGGGGCGACAACTTCGAACAGAGGAATCAAATGAATACCAAGAGGAAGAGCCATCTTGGGTGAGCGATAGGCGAGGGCTACTCGAGGCCACTGGAGCCAACATCAAGGCGAATGTGGTGGTGGCTAAAGACGGAAGTGGAAAGTACAAGACAATAACTGAAGCCCTTAAAGAGGTACCATTGAAGAGCAATACAACATTTGTGATTTATGTGAAGGAAGGAGTTTATGAAGAGCAAGTGATGGTGGACAAAAAAATGACCTATGTCATGATGATTGGAGACGGCCCGACTAAGACCAAGATCACAGCTAACAAGAATGTCCAGGATGGAACACCCACATTCAAAAGTGCTACCTTTGGTAAGTTGCCATCTAATTTATAATCATATTCATACGAATTCAATAAGTGAATTTAACATGGTTACATATTCTTAACAGCGGCGATCGGATCCAACTTCATCGGAAAAGACTTGTGGTTCGATAACTCAGCTGGACTTCACAAAGGCCAGGCCGTGGCACTCCGAGTTCAATCCGACATGTCAATCTTCTACAATTGCAGAATGGACGGCTATCAAGATACTCTCTACCCCCAAACCAAACGTCAATTCTATCGAGATTGCACAATCTCCGGTACCGTTGATTTCATCTTTGGCAATGCTGCTGCCGTGTTACAGAACTGTAAGATCTTAGTGAGGAAGCCAAAGCCGAATCAAGCATGCTTTGTCACTGCCCAAGGTCGAATGGAGAGTGATGAGCCCACTGGTATTGTCCTCCAAAATTGCGTTATCTCTTCCGACCCTGAGTTCTACCCCATTCGCCATACCAGTAAGTCCTATCTTGGTCGCCCATGGAAGAAGTATTCAAGAACCGTCATCATGCAATGCCAAATCGATGACTTGATCCATCCTGATGGTTGGATGCGATGGAGTGATGATTCTACTCTCAAGACTTTGTATTACACAGAGTTCGATAATAGAGGACCTGGTGCGGCAAAAGAAAATAGAGTTAAGTGGAAGGGAATTAAACAAATCACAGCAAAACAGGCCATCAATTTCACCCCTTCCTTGTTTATTCAAGGTGATGATTGGATCAAGAACAGTGGAATACCGTACACTGGTGGCATGATGAAAATCTAA

mRNA sequence

ATGGCGTATGACGGCGGTGGATGCAAGAACAATAAAGCTGCCTTGGTTGGGTTGTTGTCTCTGTTTTTGGTTGCCTTGGTTAATGGTGTAAGTGGCGGAGGAGAGGACTCTGGCTCCTCTAATGCTGCCATTAAGACTCTATGTGAGCCCACTACTTACCGTGAAACTTGCGAAGAAGCCCTGGTGAAGTCTAATGTTGACTCAAAAGACCCACGTGAGTTGGTCAAGGCAGGGTTCCATTTTGCTATTGAGCAGCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAAACAAGCTACCACCGACCCCATGGCGCAAAAAGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTGATAAAATCACCAATAGTTTTAATTTACACAAAATGAATGATTATCTTGAGAATCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAAGAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGATGTCAAAAGAAATGACCGCCAATGGGCTTGTGATGGTGGGTGAGGTTACATCCCTTGTGAGCACTTTGTGGGAGAAGTTGGGACTGCCCCCACAAACGGGGCGACAACTTCGAACAGAGGAATCAAATGAATACCAAGAGGAAGAGCCATCTTGGGTGAGCGATAGGCGAGGGCTACTCGAGGCCACTGGAGCCAACATCAAGGCGAATGTGGTGGTGGCTAAAGACGGAAGTGGAAAGTACAAGACAATAACTGAAGCCCTTAAAGAGGTACCATTGAAGAGCAATACAACATTTGTGATTTATGTGAAGGAAGGAGTTTATGAAGAGCAAGTGATGGTGGACAAAAAAATGACCTATGTCATGATGATTGGAGACGGCCCGACTAAGACCAAGATCACAGCTAACAAGAATGTCCAGGATGGAACACCCACATTCAAAAGTGCTACCTTTGCGGCGATCGGATCCAACTTCATCGGAAAAGACTTGTGGTTCGATAACTCAGCTGGACTTCACAAAGGCCAGGCCGTGGCACTCCGAGTTCAATCCGACATGTCAATCTTCTACAATTGCAGAATGGACGGCTATCAAGATACTCTCTACCCCCAAACCAAACGTCAATTCTATCGAGATTGCACAATCTCCGGTACCGTTGATTTCATCTTTGGCAATGCTGCTGCCGTGTTACAGAACTGTAAGATCTTAGTGAGGAAGCCAAAGCCGAATCAAGCATGCTTTGTCACTGCCCAAGGTCGAATGGAGAGTGATGAGCCCACTGGTATTGTCCTCCAAAATTGCGTTATCTCTTCCGACCCTGAGTTCTACCCCATTCGCCATACCAGTAAGTCCTATCTTGGTCGCCCATGGAAGAAGTATTCAAGAACCGTCATCATGCAATGCCAAATCGATGACTTGATCCATCCTGATGGTTGGATGCGATGGAGTGATGATTCTACTCTCAAGACTTTGTATTACACAGAGTTCGATAATAGAGGACCTGGTGCGGCAAAAGAAAATAGAGTTAAGTGGAAGGGAATTAAACAAATCACAGCAAAACAGGCCATCAATTTCACCCCTTCCTTGTTTATTCAAGGTGATGATTGGATCAAGAACAGTGGAATACCGTACACTGGTGGCATGATGAAAATCTAA

Coding sequence (CDS)

ATGGCGTATGACGGCGGTGGATGCAAGAACAATAAAGCTGCCTTGGTTGGGTTGTTGTCTCTGTTTTTGGTTGCCTTGGTTAATGGTGTAAGTGGCGGAGGAGAGGACTCTGGCTCCTCTAATGCTGCCATTAAGACTCTATGTGAGCCCACTACTTACCGTGAAACTTGCGAAGAAGCCCTGGTGAAGTCTAATGTTGACTCAAAAGACCCACGTGAGTTGGTCAAGGCAGGGTTCCATTTTGCTATTGAGCAGCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAAACAAGCTACCACCGACCCCATGGCGCAAAAAGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTGATAAAATCACCAATAGTTTTAATTTACACAAAATGAATGATTATCTTGAGAATCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAAGAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGATGTCAAAAGAAATGACCGCCAATGGGCTTGTGATGGTGGGTGAGGTTACATCCCTTGTGAGCACTTTGTGGGAGAAGTTGGGACTGCCCCCACAAACGGGGCGACAACTTCGAACAGAGGAATCAAATGAATACCAAGAGGAAGAGCCATCTTGGGTGAGCGATAGGCGAGGGCTACTCGAGGCCACTGGAGCCAACATCAAGGCGAATGTGGTGGTGGCTAAAGACGGAAGTGGAAAGTACAAGACAATAACTGAAGCCCTTAAAGAGGTACCATTGAAGAGCAATACAACATTTGTGATTTATGTGAAGGAAGGAGTTTATGAAGAGCAAGTGATGGTGGACAAAAAAATGACCTATGTCATGATGATTGGAGACGGCCCGACTAAGACCAAGATCACAGCTAACAAGAATGTCCAGGATGGAACACCCACATTCAAAAGTGCTACCTTTGCGGCGATCGGATCCAACTTCATCGGAAAAGACTTGTGGTTCGATAACTCAGCTGGACTTCACAAAGGCCAGGCCGTGGCACTCCGAGTTCAATCCGACATGTCAATCTTCTACAATTGCAGAATGGACGGCTATCAAGATACTCTCTACCCCCAAACCAAACGTCAATTCTATCGAGATTGCACAATCTCCGGTACCGTTGATTTCATCTTTGGCAATGCTGCTGCCGTGTTACAGAACTGTAAGATCTTAGTGAGGAAGCCAAAGCCGAATCAAGCATGCTTTGTCACTGCCCAAGGTCGAATGGAGAGTGATGAGCCCACTGGTATTGTCCTCCAAAATTGCGTTATCTCTTCCGACCCTGAGTTCTACCCCATTCGCCATACCAGTAAGTCCTATCTTGGTCGCCCATGGAAGAAGTATTCAAGAACCGTCATCATGCAATGCCAAATCGATGACTTGATCCATCCTGATGGTTGGATGCGATGGAGTGATGATTCTACTCTCAAGACTTTGTATTACACAGAGTTCGATAATAGAGGACCTGGTGCGGCAAAAGAAAATAGAGTTAAGTGGAAGGGAATTAAACAAATCACAGCAAAACAGGCCATCAATTTCACCCCTTCCTTGTTTATTCAAGGTGATGATTGGATCAAGAACAGTGGAATACCGTACACTGGTGGCATGATGAAAATCTAA

Protein sequence

MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAAIKTLCEPTTYRETCEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDACDELMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLKMSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPSLFIQGDDWIKNSGIPYTGGMMKI
Homology
BLAST of CmoCh06G015190 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 556.6 bits (1433), Expect = 3.2e-157
Identity = 272/574 (47.39%), Postives = 387/574 (67.42%), Query Frame = 0

Query: 3   YDGGGCKNNKAALVGLLSLFLVALV------------NGVSGGGEDSGSSNAAIKTLCEP 62
           YD    +  +  ++ + S+ L+++V            +G S G  +  +S  A+K +C P
Sbjct: 5   YDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAP 64

Query: 63  TTYRETCEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVD 122
           T YR+TCE+ L+K+  ++ DP ELVK  F+  ++Q+  A   S T+ +   D   + A+D
Sbjct: 65  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 124

Query: 123 ACDELMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGD 182
            C ELMDYA+D+L  SF+++   F  H +++ L NLRIWLSAA++++ETCL+GF+   G+
Sbjct: 125 QCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGN 184

Query: 183 TGEKMKNLLKMSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEP 242
            GE MK  LK + E+T NGL ++ E+++ V     ++ +P    R+L  E         P
Sbjct: 185 AGETMKKALKTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLLAEGF-------P 244

Query: 243 SWVSDR-RGLLEATGA--NIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGV 302
           SWV  R R LL+A  A  ++K ++VVA+DGSG+YKTI EAL+ VP K NTTFV+++K G+
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGL 304

Query: 303 YEEQVMVDKKMTYVMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDN 362
           Y+E V V+K M++++ IGDGP KT I+ NKN +DG  T+++AT A +G+ FI K++ F+N
Sbjct: 305 YKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFEN 364

Query: 363 SAGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAA 422
           +AG  K QAVA+RVQSD SIF+NCR DGYQDTLY  + RQF+RDCTISGT+DF+FG+AAA
Sbjct: 365 TAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAA 424

Query: 423 VLQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGR 482
           V QNC +LVRKP PNQAC +TA GR +  E TG V Q C I+ +P++  ++ TSK+YLGR
Sbjct: 425 VFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGR 484

Query: 483 PWKKYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIK 542
           PWK+YSRT+IM   I D + P GW  W  D  LKTL+Y+E  N GPG+A  NRV W GIK
Sbjct: 485 PWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIK 544

Query: 543 QITAKQAINFTPSLFIQGDDWIKNSGIPYTGGMM 562
            ++ +  + FTP+ +IQGDDWI   G+PYT G++
Sbjct: 545 TLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of CmoCh06G015190 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 3.1e-152
Identity = 267/570 (46.84%), Postives = 379/570 (66.49%), Query Frame = 0

Query: 3   YDGGGCKNNKA-ALVGLLSLFLVALVNGV----------SGGGEDSGSSNAAIKTLCEPT 62
           YD    K  K   ++ + S+ L+++V  V          + G E+  +S  AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 63  TYRETCEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDA 122
            Y+ETCE+ L K   D+ DP ELVK  F+  ++Q+      S T+ +   DP A+ A+D 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 123 CDELMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDT 182
           C ELMDYAI +L  SF+++   F  HK+++ L  LRIWLSA +++++TCLDGF+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 183 GEKMKNLLKMSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPS 242
           GE +K  LK + ++T NGL MV E+++ +     ++ +P    R+L ++E        PS
Sbjct: 185 GETIKKALKTAVQLTHNGLAMVTEMSNYLG----QMQIPEMNSRRLLSQEF-------PS 244

Query: 243 WVSDR-RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEE 302
           W+  R R LL A  + +K ++VVA+DGSG+YKTI EAL  VP K NTTFV+++KEG+Y+E
Sbjct: 245 WMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKE 304

Query: 303 QVMVDKKMTYVMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAG 362
            V V++ MT+++ IGDGP KT I+ +K+ +DG  T+K+AT A +G +FI K++ F+N+AG
Sbjct: 305 YVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAG 364

Query: 363 LHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQ 422
             K QAVA+RV +D SIFYNC+ DGYQDTLY  + RQFYRDCTISGT+DF+FG+AAAV Q
Sbjct: 365 AIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQ 424

Query: 423 NCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWK 482
           NC +LVRKP  NQAC +TA GR +  E TG VLQ C I  +P++  ++  SK+YLGRPWK
Sbjct: 425 NCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWK 484

Query: 483 KYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQIT 542
           +YSRT+IM   I D + P+GW  W  +  L TL+Y+E  N GPGAA   RV W GIK+++
Sbjct: 485 EYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLS 544

Query: 543 AKQAINFTPSLFIQGDDWIKNSGIPYTGGM 561
            ++ + FTP+ +IQGD WI   G+PY  G+
Sbjct: 545 DEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmoCh06G015190 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 7.7e-143
Identity = 255/568 (44.89%), Postives = 370/568 (65.14%), Query Frame = 0

Query: 6   GGCKNNKAALVGLLSLFLVALV--NGVSGGGEDSGS----------SNAAIKTLCEPTTY 65
           G  K  K  + G+++  LV +V   G++     S S          +  A++ +C PT Y
Sbjct: 5   GELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDY 64

Query: 66  RETCEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQATTDPMAQKAVDA 125
           +ETC  +L+K++ DS  P +L+K GF+  I  ++ +I  A+     +A  D   + A++ 
Sbjct: 65  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 124

Query: 126 CDELMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDT 185
           C++LM+ A DDL    D   + F++ ++ D++E+LR+WLS ++ YQ+TC+D FE      
Sbjct: 125 CEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKL 184

Query: 186 GEKMKNLLKMSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPS 245
            + M+ + K S+E+T+NGL M+  +++L+   +   G+    G+  R   S E  +  PS
Sbjct: 185 SQDMQKIFKTSRELTSNGLAMITNISNLLGE-FNVTGVTGDLGKYARKLLSAE--DGIPS 244

Query: 246 WVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQ 305
           WV      L AT   +KANVVVA DGSG+YKTI EAL  VP  +   FVIY+K+GVY E+
Sbjct: 245 WVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEK 304

Query: 306 VMVDKKMTYVMMIGDGPTKTKITANKNVQDG-TPTFKSATFAAIGSNFIGKDLWFDNSAG 365
           V V KKMT+V  IGDGPTKTKIT + N   G   T+ +AT A  G NF  K++ F+N+AG
Sbjct: 305 VDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAG 364

Query: 366 LHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQ 425
               QAVALRV +D+++FYNC++DGYQDTLY  + RQF+RDCT+SGTVDFIFG+   VLQ
Sbjct: 365 PEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQ 424

Query: 426 NCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWK 485
           NC I+VRKP  +Q+C +TAQGR +  E TG+VLQNC I+ +P + P++  +K+YLGRPWK
Sbjct: 425 NCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWK 484

Query: 486 KYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQIT 545
           ++SRT+IM   IDD+I P GW+ W+ D  L TLYY E++N GPG+ +  RVKW GIK+++
Sbjct: 485 EFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLS 544

Query: 546 AKQAINFTPSLFIQGDDWIKNSGIPYTG 559
            KQA+ FTP+ F++G+ WI  + +PY G
Sbjct: 545 PKQALRFTPARFLRGNLWIPPNRVPYMG 568

BLAST of CmoCh06G015190 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 468.8 bits (1205), Expect = 8.8e-131
Identity = 242/569 (42.53%), Postives = 362/569 (63.62%), Query Frame = 0

Query: 7   GCKNNKAALVGLLSLFLVALVNGVS------GGGEDSG---SSNAAIKTLCEPTTYRETC 66
           G K  K  + G +S FLV +V  V+         +D      +  A++ +C PT +++TC
Sbjct: 5   GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTC 64

Query: 67  EEALVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQATTDPMAQKAVDACDEL 126
             +L+ ++ DS DP +L+K GF   I+ +  ++  A+     +A  +P A+ A + C++L
Sbjct: 65  VNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKL 124

Query: 127 MDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 186
           M  AIDDL    D   + F++ ++  ++E+LR+WLS ++ +Q+TC+D F  +  +  + M
Sbjct: 125 MIDAIDDLKKCMD---HGFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 184

Query: 187 KNLLKMSKEMTANGLVMVGEVTSLV--STLWEKLGLPPQTGRQLRTEESNEYQEEEPSWV 246
             + K S+E+++N L MV  +++L+  S L    G   +  R+L + E     +  P+WV
Sbjct: 185 LKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTE-----DSIPTWV 244

Query: 247 S-DRRGLLEATGAN---IKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYE 306
             + R L+ A G     +KAN VVA+DG+G++KTIT+AL  VP  +   F+I++KEG+Y+
Sbjct: 245 GPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYK 304

Query: 307 EQVMVDKKMTYVMMIGDGPTKTKITANKNVQDG-TPTFKSATFAAIGSNFIGKDLWFDNS 366
           E+V V KKM +V  IGDGP KT IT + N   G   TF +AT    G +F  K++  +N+
Sbjct: 305 EKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENT 364

Query: 367 AGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAV 426
           AG   GQAVALRV +D ++F++C++DG+QDTLY  + RQFYRDCT+SGTVDFIFG+A  +
Sbjct: 365 AGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCI 424

Query: 427 LQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRP 486
           LQNCKI+VRKP   Q C VTAQGR    E TG+VL  C I+ DP + P++  +K+YLGRP
Sbjct: 425 LQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRP 484

Query: 487 WKKYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQ 546
           WK++SRT+IM+  IDD+I P GW+ WS D  LKTLYY E  N GPG+ +  RVKW GIK+
Sbjct: 485 WKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKK 544

Query: 547 ITAKQAINFTPSLFIQGDDWIKNSGIPYT 558
           +T + A+ +T   F++GD WI  + +PYT
Sbjct: 545 LTPQDALLYTGDRFLRGDTWIPQTQVPYT 565

BLAST of CmoCh06G015190 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.3e-113
Identity = 242/701 (34.52%), Postives = 366/701 (52.21%), Query Frame = 0

Query: 11  NKAALVGLLSLFLVALVNGVSGGGEDSGS--------------SNAAIKTLCEPTTYRET 70
           NK  L+G+ +L ++A+V  V     +S S              +   +  +C  T Y++ 
Sbjct: 2   NKYVLLGVTAL-IMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQD 61

Query: 71  CEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAIANS--PTLKQATTDPMAQKAVDACDE 130
           C  +L  + V S DPR L+++ F  AI  +++ I         +A  D   ++A++ C E
Sbjct: 62  CTTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCRE 121

Query: 131 LMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEK 190
           LMD AIDDL  + DK    F   +++D++E+L +WLS ++TYQ+TC+DGFE +  +    
Sbjct: 122 LMDDAIDDLRKTRDKF-RGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 181

Query: 191 MKNLLKMSKEMTANGLVMVGEVTSLV-----------------------STLWEKLG--- 250
           M+ +++  + +T+NGL +   +  L+                       S+  E +G   
Sbjct: 182 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 241

Query: 251 ----------------------------------------LPPQTGRQLRTEE------- 310
                                                   L     +Q+ + E       
Sbjct: 242 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 301

Query: 311 -------------------SNEYQE----------------------------------- 370
                              S+E Q                                    
Sbjct: 302 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 361

Query: 371 --------EEPSWVSDR-RGLLEATGAN--IKANVVVAKDGSGKYKTITEALKEVPLKSN 430
                   E P WV+   R LL     N  IKANVVVAKDGSGK KTI +AL  VP+K+ 
Sbjct: 362 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNT 421

Query: 431 TTFVIYVKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITAN-KNVQDGTPTFKSATFAAIG 490
             FVI++KEGVY+E+V V KKM +VM +GDGPTKT IT +   + D   T+++A+ A  G
Sbjct: 422 KKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNG 481

Query: 491 SNFIGKDLWFDNSAGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTIS 550
             F+ KD+ F+N+AG  + QAVALRV +D ++F+NC M+GYQDTLY  T RQFYR+C +S
Sbjct: 482 DYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVS 541

Query: 551 GTVDFIFGNAAAVLQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFY 557
           GT+DF+FG+A AV QNC+ ++R+P  +Q C VTAQGR +  E TGIV+ N  I+ D  + 
Sbjct: 542 GTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYL 601

BLAST of CmoCh06G015190 vs. ExPASy TrEMBL
Match: A0A6J1GCP0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452765 PE=3 SV=1)

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0

Query: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAAIKTLCEPTTYRETCEEA 60
           MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAAIKTLCEPTTYRETCEEA
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAAIKTLCEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 MSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLL 240
           MSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLL
Sbjct: 181 MSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY 300

Query: 301 VMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALR 360
           VMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALR
Sbjct: 301 VMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPK 420
           VQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPK
Sbjct: 361 VQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPK 420

Query: 421 PNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQC 480
           PNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQC
Sbjct: 421 PNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQC 480

Query: 481 QIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPS 540
           QIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPS
Sbjct: 481 QIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPS 540

Query: 541 LFIQGDDWIKNSGIPYTGGMMKI 564
           LFIQGDDWIKNSGIPYTGGMMKI
Sbjct: 541 LFIQGDDWIKNSGIPYTGGMMKI 563

BLAST of CmoCh06G015190 vs. ExPASy TrEMBL
Match: A0A6J1GBF1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452639 PE=3 SV=1)

HSP 1 Score: 1069.7 bits (2765), Expect = 4.2e-309
Identity = 523/563 (92.90%), Postives = 543/563 (96.45%), Query Frame = 0

Query: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAAIKTLCEPTTYRETCEEA 60
           MAYDGGGCKNNKAALVGLLSLF VALVNGVSG GE+ GSSNAAIKTLCEPTTYRETCEE 
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFFVALVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQA TDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKIT+SF++H++N YLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 MSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLL 240
            S+EMTANGLVMVGEVTSLVSTLWEKLGL PQ+GRQLRTEESNEYQ  EPSWVSDRRGLL
Sbjct: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQSGRQLRTEESNEYQAGEPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQV+VDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300

Query: 301 VMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALR 360
           VM+IGDGPTKTKITA+KNV DGTPTFKSATFAAIGSNFIGKDLWFDNSAG  K QAVALR
Sbjct: 301 VMVIGDGPTKTKITASKNVIDGTPTFKSATFAAIGSNFIGKDLWFDNSAGPKKHQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPK 420
           VQSDMSIFYNCRMD YQDTLYPQTKRQFYRDCTISGTVDFIFGNAA VLQNCKILVRKP 
Sbjct: 361 VQSDMSIFYNCRMDAYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPM 420

Query: 421 PNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQC 480
           PNQ CFVTAQGR+E DEPTGIVLQNC+IS+DPEFYPIRHTSK +LGRPWKKYSRTVIMQC
Sbjct: 421 PNQTCFVTAQGRVEIDEPTGIVLQNCIISADPEFYPIRHTSKIFLGRPWKKYSRTVIMQC 480

Query: 481 QIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPS 540
           Q+DDLIHP+GWMRWSDDSTLKTL+YTEFDNRGPGAAKENRVKW+GIKQITAKQAINFTPS
Sbjct: 481 QLDDLIHPEGWMRWSDDSTLKTLFYTEFDNRGPGAAKENRVKWEGIKQITAKQAINFTPS 540

Query: 541 LFIQGDDWIKNSGIPYTGGMMKI 564
           LFIQGDDWIK+SGIPYTGGMMKI
Sbjct: 541 LFIQGDDWIKHSGIPYTGGMMKI 563

BLAST of CmoCh06G015190 vs. ExPASy TrEMBL
Match: A0A6J1IFH7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472306 PE=3 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 5.1e-307
Identity = 528/567 (93.12%), Postives = 540/567 (95.24%), Query Frame = 0

Query: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAA-IKTLCEPTTYRETCEE 60
           MAYD GGCK NKAALVGLLSLF VALVNGVSG GED GSSNAA IKTLCEPTTYRETCE+
Sbjct: 1   MAYDSGGCKINKAALVGLLSLFFVALVNGVSGVGEDHGSSNAATIKTLCEPTTYRETCEK 60

Query: 61  ALVKSNVDSKDPRELVKAGFHFAIEQLKAAI---ANSPTLKQATTDPMAQKAVDACDELM 120
            LVKSNVDSKDPRELVKAGFHFAIEQLKA +    +S TLKQA TDPMAQKAVDACDELM
Sbjct: 61  VLVKSNVDSKDPRELVKAGFHFAIEQLKAQLMLATSSSTLKQAATDPMAQKAVDACDELM 120

Query: 121 DYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMK 180
           DYAIDDLLTSF+KIT+SF+ HKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMK
Sbjct: 121 DYAIDDLLTSFNKITDSFSFHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMK 180

Query: 181 NLLKMSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDR 240
           NLLK SKEMTANGLVMVGEVTSLVSTLW+KLGLPPQTGRQLRTE SNE ++EEPSWVSDR
Sbjct: 181 NLLKTSKEMTANGLVMVGEVTSLVSTLWQKLGLPPQTGRQLRTEHSNE-EKEEPSWVSDR 240

Query: 241 RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDK 300
           RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDK
Sbjct: 241 RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDK 300

Query: 301 KMTYVMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQA 360
           KMTYVM+IGDGPTKTKITA+KNV DGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQA
Sbjct: 301 KMTYVMVIGDGPTKTKITASKNVVDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQA 360

Query: 361 VALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILV 420
           VALRVQSDMSIFYNCRMDGYQDTLYP   RQFYRDCTISGTVDFIFGNAAAVLQNCKILV
Sbjct: 361 VALRVQSDMSIFYNCRMDGYQDTLYPHANRQFYRDCTISGTVDFIFGNAAAVLQNCKILV 420

Query: 421 RKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTV 480
           RKPKPNQACFVTAQGRMESDEPTGIVLQNC ISSDPEFYPIRHTSKSYLGRPWKKYSRTV
Sbjct: 421 RKPKPNQACFVTAQGRMESDEPTGIVLQNCTISSDPEFYPIRHTSKSYLGRPWKKYSRTV 480

Query: 481 IMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAIN 540
           IMQCQIDDLIHPDGWMRWSDDSTL TLYYTEFDNRGPGAAKENRVKWKGIKQITA QAIN
Sbjct: 481 IMQCQIDDLIHPDGWMRWSDDSTLNTLYYTEFDNRGPGAAKENRVKWKGIKQITATQAIN 540

Query: 541 FTPSLFIQGDDWIKNSGIPYTGGMMKI 564
           FTPSLFI GD WIK+SGIPYTGGMMKI
Sbjct: 541 FTPSLFIHGDSWIKSSGIPYTGGMMKI 566

BLAST of CmoCh06G015190 vs. ExPASy TrEMBL
Match: A0A6J1IFP3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472354 PE=3 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.5e-292
Identity = 496/563 (88.10%), Postives = 528/563 (93.78%), Query Frame = 0

Query: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAAIKTLCEPTTYRETCEEA 60
           MAYD GGCK+NKAALVGLLSLFLVALVNGV+G GED GSSNA IKTLCEPTTYRETCE+ 
Sbjct: 1   MAYDRGGCKDNKAALVGLLSLFLVALVNGVNGAGEDHGSSNADIKTLCEPTTYRETCEKV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTL+QA TDP+AQ AVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLRQAATDPLAQNAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSF+KIT+SF++H++NDYLENLRIWLSAALTYQ+TCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLK 180

Query: 181 MSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLL 240
            SKEMTANGLVMVGEVTSLV+TLW+KLGL  QTGRQLRTE SNE ++EEPSWVSDRRGLL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVTTLWQKLGL-LQTGRQLRTEHSNE-EKEEPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY 300

Query: 301 VMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALR 360
           VM+IGDGPTKTKIT +KNV DGT TFKSATFAA+GSNFIGKDLWFDNSAGL+KGQAVALR
Sbjct: 301 VMVIGDGPTKTKITGSKNVVDGTTTFKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPK 420
           VQSDMSIFYNCRMDGYQDT YP   RQFYRDCTISGTVDFIFGNAA VLQNCKILVRKPK
Sbjct: 361 VQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420

Query: 421 PNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQC 480
           P Q C +TAQGRMESDEPTGIVLQNC ISSDP++YP+RHTSKSYLGRPWK+YSRTV+MQ 
Sbjct: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQS 480

Query: 481 QIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPS 540
           QIDDLI P+GW+ W+ D  L TLYYTEFDNRGPGAAKENRVKWKGIKQITAK+AINFTPS
Sbjct: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPS 540

Query: 541 LFIQGDDWIKNSGIPYTGGMMKI 564
           LFI GD WIKN+GI YTG +MKI
Sbjct: 541 LFIHGDSWIKNTGISYTGALMKI 561

BLAST of CmoCh06G015190 vs. ExPASy TrEMBL
Match: A0A6J1I9A9 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472265 PE=3 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 8.5e-286
Identity = 489/562 (87.01%), Postives = 521/562 (92.70%), Query Frame = 0

Query: 6   GGC----KNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAAIKTLCEPTTYRETCEEAL 65
           GGC     +  AALVGLLSLFLVALVNGV+G GED GSSNA IKTLCEPTTYRETCE+ L
Sbjct: 13  GGCYIHPLSGTAALVGLLSLFLVALVNGVNGAGEDHGSSNADIKTLCEPTTYRETCEKVL 72

Query: 66  VKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDACDELMDYAID 125
           VKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTL+QA TDP+AQ AVDACDELMDYAID
Sbjct: 73  VKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLRQAATDPLAQNAVDACDELMDYAID 132

Query: 126 DLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLKM 185
           DLLTSF+KIT+SF++H++NDYLENLRIWLSAALTYQ+TCLDGFENVPGDTGEKMKNLLK 
Sbjct: 133 DLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKT 192

Query: 186 SKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLLE 245
           SKEMTANGLVMVGEVTSLV+TLW+KLGL  QTGRQLRTE SNE ++EEPSWVSDRRGLLE
Sbjct: 193 SKEMTANGLVMVGEVTSLVTTLWQKLGL-LQTGRQLRTEHSNE-EKEEPSWVSDRRGLLE 252

Query: 246 ATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYV 305
           ATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYV
Sbjct: 253 ATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYV 312

Query: 306 MMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALRV 365
           M+IGDGPTKTKIT +KNV DGT TFKSATFAA+GSNFIGKDLWFDNSAGL+KGQAVALRV
Sbjct: 313 MVIGDGPTKTKITGSKNVVDGTTTFKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRV 372

Query: 366 QSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPKP 425
           QSDMSIFYNCRMDGYQDT YP   RQFYRDCTISGTVDFIFGNAA VLQNCKILVRKPKP
Sbjct: 373 QSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKP 432

Query: 426 NQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQCQ 485
            Q C +TAQGRMESDEPTGIVLQNC ISSDP++YP+RHTSKSYLGRPWK+YSRTV+MQ Q
Sbjct: 433 KQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQ 492

Query: 486 IDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPSL 545
           IDDLI P+GW+ W+ D  L TLYYTEFDNRGPGAAKENRVKWKGIKQITAK+AINFTPSL
Sbjct: 493 IDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPSL 552

Query: 546 FIQGDDWIKNSGIPYTGGMMKI 564
           FI GD WIKN+GI YTG +MKI
Sbjct: 553 FIHGDSWIKNTGISYTGALMKI 572

BLAST of CmoCh06G015190 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 556.6 bits (1433), Expect = 2.3e-158
Identity = 272/574 (47.39%), Postives = 387/574 (67.42%), Query Frame = 0

Query: 3   YDGGGCKNNKAALVGLLSLFLVALV------------NGVSGGGEDSGSSNAAIKTLCEP 62
           YD    +  +  ++ + S+ L+++V            +G S G  +  +S  A+K +C P
Sbjct: 5   YDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAP 64

Query: 63  TTYRETCEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVD 122
           T YR+TCE+ L+K+  ++ DP ELVK  F+  ++Q+  A   S T+ +   D   + A+D
Sbjct: 65  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 124

Query: 123 ACDELMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGD 182
            C ELMDYA+D+L  SF+++   F  H +++ L NLRIWLSAA++++ETCL+GF+   G+
Sbjct: 125 QCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGN 184

Query: 183 TGEKMKNLLKMSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEP 242
            GE MK  LK + E+T NGL ++ E+++ V     ++ +P    R+L  E         P
Sbjct: 185 AGETMKKALKTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLLAEGF-------P 244

Query: 243 SWVSDR-RGLLEATGA--NIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGV 302
           SWV  R R LL+A  A  ++K ++VVA+DGSG+YKTI EAL+ VP K NTTFV+++K G+
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGL 304

Query: 303 YEEQVMVDKKMTYVMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDN 362
           Y+E V V+K M++++ IGDGP KT I+ NKN +DG  T+++AT A +G+ FI K++ F+N
Sbjct: 305 YKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFEN 364

Query: 363 SAGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAA 422
           +AG  K QAVA+RVQSD SIF+NCR DGYQDTLY  + RQF+RDCTISGT+DF+FG+AAA
Sbjct: 365 TAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAA 424

Query: 423 VLQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGR 482
           V QNC +LVRKP PNQAC +TA GR +  E TG V Q C I+ +P++  ++ TSK+YLGR
Sbjct: 425 VFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGR 484

Query: 483 PWKKYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIK 542
           PWK+YSRT+IM   I D + P GW  W  D  LKTL+Y+E  N GPG+A  NRV W GIK
Sbjct: 485 PWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIK 544

Query: 543 QITAKQAINFTPSLFIQGDDWIKNSGIPYTGGMM 562
            ++ +  + FTP+ +IQGDDWI   G+PYT G++
Sbjct: 545 TLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of CmoCh06G015190 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 540.0 bits (1390), Expect = 2.2e-153
Identity = 267/570 (46.84%), Postives = 379/570 (66.49%), Query Frame = 0

Query: 3   YDGGGCKNNKA-ALVGLLSLFLVALVNGV----------SGGGEDSGSSNAAIKTLCEPT 62
           YD    K  K   ++ + S+ L+++V  V          + G E+  +S  AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 63  TYRETCEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDA 122
            Y+ETCE+ L K   D+ DP ELVK  F+  ++Q+      S T+ +   DP A+ A+D 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 123 CDELMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDT 182
           C ELMDYAI +L  SF+++   F  HK+++ L  LRIWLSA +++++TCLDGF+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 183 GEKMKNLLKMSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPS 242
           GE +K  LK + ++T NGL MV E+++ +     ++ +P    R+L ++E        PS
Sbjct: 185 GETIKKALKTAVQLTHNGLAMVTEMSNYLG----QMQIPEMNSRRLLSQEF-------PS 244

Query: 243 WVSDR-RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEE 302
           W+  R R LL A  + +K ++VVA+DGSG+YKTI EAL  VP K NTTFV+++KEG+Y+E
Sbjct: 245 WMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKE 304

Query: 303 QVMVDKKMTYVMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAG 362
            V V++ MT+++ IGDGP KT I+ +K+ +DG  T+K+AT A +G +FI K++ F+N+AG
Sbjct: 305 YVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAG 364

Query: 363 LHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQ 422
             K QAVA+RV +D SIFYNC+ DGYQDTLY  + RQFYRDCTISGT+DF+FG+AAAV Q
Sbjct: 365 AIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQ 424

Query: 423 NCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWK 482
           NC +LVRKP  NQAC +TA GR +  E TG VLQ C I  +P++  ++  SK+YLGRPWK
Sbjct: 425 NCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWK 484

Query: 483 KYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQIT 542
           +YSRT+IM   I D + P+GW  W  +  L TL+Y+E  N GPGAA   RV W GIK+++
Sbjct: 485 EYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLS 544

Query: 543 AKQAINFTPSLFIQGDDWIKNSGIPYTGGM 561
            ++ + FTP+ +IQGD WI   G+PY  G+
Sbjct: 545 DEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmoCh06G015190 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 508.8 bits (1309), Expect = 5.5e-144
Identity = 255/568 (44.89%), Postives = 370/568 (65.14%), Query Frame = 0

Query: 6   GGCKNNKAALVGLLSLFLVALV--NGVSGGGEDSGS----------SNAAIKTLCEPTTY 65
           G  K  K  + G+++  LV +V   G++     S S          +  A++ +C PT Y
Sbjct: 5   GELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDY 64

Query: 66  RETCEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQATTDPMAQKAVDA 125
           +ETC  +L+K++ DS  P +L+K GF+  I  ++ +I  A+     +A  D   + A++ 
Sbjct: 65  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 124

Query: 126 CDELMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDT 185
           C++LM+ A DDL    D   + F++ ++ D++E+LR+WLS ++ YQ+TC+D FE      
Sbjct: 125 CEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKL 184

Query: 186 GEKMKNLLKMSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPS 245
            + M+ + K S+E+T+NGL M+  +++L+   +   G+    G+  R   S E  +  PS
Sbjct: 185 SQDMQKIFKTSRELTSNGLAMITNISNLLGE-FNVTGVTGDLGKYARKLLSAE--DGIPS 244

Query: 246 WVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQ 305
           WV      L AT   +KANVVVA DGSG+YKTI EAL  VP  +   FVIY+K+GVY E+
Sbjct: 245 WVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEK 304

Query: 306 VMVDKKMTYVMMIGDGPTKTKITANKNVQDG-TPTFKSATFAAIGSNFIGKDLWFDNSAG 365
           V V KKMT+V  IGDGPTKTKIT + N   G   T+ +AT A  G NF  K++ F+N+AG
Sbjct: 305 VDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAG 364

Query: 366 LHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQ 425
               QAVALRV +D+++FYNC++DGYQDTLY  + RQF+RDCT+SGTVDFIFG+   VLQ
Sbjct: 365 PEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQ 424

Query: 426 NCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWK 485
           NC I+VRKP  +Q+C +TAQGR +  E TG+VLQNC I+ +P + P++  +K+YLGRPWK
Sbjct: 425 NCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWK 484

Query: 486 KYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQIT 545
           ++SRT+IM   IDD+I P GW+ W+ D  L TLYY E++N GPG+ +  RVKW GIK+++
Sbjct: 485 EFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLS 544

Query: 546 AKQAINFTPSLFIQGDDWIKNSGIPYTG 559
            KQA+ FTP+ F++G+ WI  + +PY G
Sbjct: 545 PKQALRFTPARFLRGNLWIPPNRVPYMG 568

BLAST of CmoCh06G015190 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 468.8 bits (1205), Expect = 6.3e-132
Identity = 242/569 (42.53%), Postives = 362/569 (63.62%), Query Frame = 0

Query: 7   GCKNNKAALVGLLSLFLVALVNGVS------GGGEDSG---SSNAAIKTLCEPTTYRETC 66
           G K  K  + G +S FLV +V  V+         +D      +  A++ +C PT +++TC
Sbjct: 5   GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTC 64

Query: 67  EEALVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQATTDPMAQKAVDACDEL 126
             +L+ ++ DS DP +L+K GF   I+ +  ++  A+     +A  +P A+ A + C++L
Sbjct: 65  VNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKL 124

Query: 127 MDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 186
           M  AIDDL    D   + F++ ++  ++E+LR+WLS ++ +Q+TC+D F  +  +  + M
Sbjct: 125 MIDAIDDLKKCMD---HGFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 184

Query: 187 KNLLKMSKEMTANGLVMVGEVTSLV--STLWEKLGLPPQTGRQLRTEESNEYQEEEPSWV 246
             + K S+E+++N L MV  +++L+  S L    G   +  R+L + E     +  P+WV
Sbjct: 185 LKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTE-----DSIPTWV 244

Query: 247 S-DRRGLLEATGAN---IKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYE 306
             + R L+ A G     +KAN VVA+DG+G++KTIT+AL  VP  +   F+I++KEG+Y+
Sbjct: 245 GPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYK 304

Query: 307 EQVMVDKKMTYVMMIGDGPTKTKITANKNVQDG-TPTFKSATFAAIGSNFIGKDLWFDNS 366
           E+V V KKM +V  IGDGP KT IT + N   G   TF +AT    G +F  K++  +N+
Sbjct: 305 EKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENT 364

Query: 367 AGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAV 426
           AG   GQAVALRV +D ++F++C++DG+QDTLY  + RQFYRDCT+SGTVDFIFG+A  +
Sbjct: 365 AGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCI 424

Query: 427 LQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRP 486
           LQNCKI+VRKP   Q C VTAQGR    E TG+VL  C I+ DP + P++  +K+YLGRP
Sbjct: 425 LQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRP 484

Query: 487 WKKYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQ 546
           WK++SRT+IM+  IDD+I P GW+ WS D  LKTLYY E  N GPG+ +  RVKW GIK+
Sbjct: 485 WKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKK 544

Query: 547 ITAKQAINFTPSLFIQGDDWIKNSGIPYT 558
           +T + A+ +T   F++GD WI  + +PYT
Sbjct: 545 LTPQDALLYTGDRFLRGDTWIPQTQVPYT 565

BLAST of CmoCh06G015190 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 409.5 bits (1051), Expect = 4.5e-114
Identity = 242/701 (34.52%), Postives = 366/701 (52.21%), Query Frame = 0

Query: 11  NKAALVGLLSLFLVALVNGVSGGGEDSGS--------------SNAAIKTLCEPTTYRET 70
           NK  L+G+ +L ++A+V  V     +S S              +   +  +C  T Y++ 
Sbjct: 2   NKYVLLGVTAL-IMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQD 61

Query: 71  CEEALVKSNVDSKDPRELVKAGFHFAIEQLKAAIANS--PTLKQATTDPMAQKAVDACDE 130
           C  +L  + V S DPR L+++ F  AI  +++ I         +A  D   ++A++ C E
Sbjct: 62  CTTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCRE 121

Query: 131 LMDYAIDDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEK 190
           LMD AIDDL  + DK    F   +++D++E+L +WLS ++TYQ+TC+DGFE +  +    
Sbjct: 122 LMDDAIDDLRKTRDKF-RGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 181

Query: 191 MKNLLKMSKEMTANGLVMVGEVTSLV-----------------------STLWEKLG--- 250
           M+ +++  + +T+NGL +   +  L+                       S+  E +G   
Sbjct: 182 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 241

Query: 251 ----------------------------------------LPPQTGRQLRTEE------- 310
                                                   L     +Q+ + E       
Sbjct: 242 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 301

Query: 311 -------------------SNEYQE----------------------------------- 370
                              S+E Q                                    
Sbjct: 302 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 361

Query: 371 --------EEPSWVSDR-RGLLEATGAN--IKANVVVAKDGSGKYKTITEALKEVPLKSN 430
                   E P WV+   R LL     N  IKANVVVAKDGSGK KTI +AL  VP+K+ 
Sbjct: 362 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNT 421

Query: 431 TTFVIYVKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITAN-KNVQDGTPTFKSATFAAIG 490
             FVI++KEGVY+E+V V KKM +VM +GDGPTKT IT +   + D   T+++A+ A  G
Sbjct: 422 KKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNG 481

Query: 491 SNFIGKDLWFDNSAGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTIS 550
             F+ KD+ F+N+AG  + QAVALRV +D ++F+NC M+GYQDTLY  T RQFYR+C +S
Sbjct: 482 DYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVS 541

Query: 551 GTVDFIFGNAAAVLQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFY 557
           GT+DF+FG+A AV QNC+ ++R+P  +Q C VTAQGR +  E TGIV+ N  I+ D  + 
Sbjct: 542 GTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYL 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GX863.2e-15747.39Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z83.1e-15246.84Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ217.7e-14344.89Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA18.8e-13142.53Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
O234476.3e-11334.52Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GCP00.0e+00100.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452765 PE=3 SV=1[more]
A0A6J1GBF14.2e-30992.90Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452639 PE=3 SV=1[more]
A0A6J1IFH75.1e-30793.12Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472306 PE=3 SV=1[more]
A0A6J1IFP33.5e-29288.10Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472354 PE=3 SV=1[more]
A0A6J1I9A98.5e-28687.01Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472265 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G05610.12.3e-15847.39Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.12.2e-15346.84Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.15.5e-14444.89Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.16.3e-13242.53Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.14.5e-11434.52Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 39..192
e-value: 2.6E-32
score: 123.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 43..187
e-value: 6.4E-23
score: 81.8
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 20..192
e-value: 2.8E-23
score: 80.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 39..196
e-value: 7.3E-31
score: 109.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 43..195
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 244..560
e-value: 1.7E-118
score: 397.6
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 250..546
e-value: 2.8E-137
score: 456.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 212..231
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 33..560
NoneNo IPR availablePANTHERPTHR31707:SF131PECTINESTERASEcoord: 33..560
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 46..198
e-value: 8.81471E-40
score: 140.266
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 394..403
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 247..559

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G015190.1CmoCh06G015190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity