CmoCh06G015160 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh06G015160
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pectinesterase
Location	Cmo_Chr06: 10803307 .. 10805041 (-)
RNA-Seq Expression	CmoCh06G015160
Synteny	CmoCh06G015160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATGCAACAACAAGAAGGCTGCTCTGGTTGGCTTGTTATCTCTGGTCCTAGTTGCCATGGTTAACGGTGTGAGTGGCTCGGAAGAAGAGATTAAGACTCTGTGTGAACCTACTGATTACCGTGAAACTTGCGAGCAAGCCTTGGTGAATTCACATACTGACTCGGAAGACCCACGTGAGTTAATGAAGGCAGGATTCCATGCTGCCATTGAGAATCTCAAATCGGTTATTGGAAATTCCATCACTATGAAAGAAGCTTCCTCTGACCCTATGGTGAAGAAGGCACTTGATGATTGCAACGAGCTCATGGATTATGCAATTGATGATCTTTTATTATCGTATGATAAAATCACCGCTAATTTTGATTACAATAAAGTGAATGATTATACTGAGAATCTTAAGATTTGGTTGAGTGGTGCATTGACGTATCAAGATACTTGTTTGGACGGGTTTGAGAATGTTACTGGTGATACTGGTGAAAAGATGCAGAATTTGTTGAAGATACCGAAGGAAATGACCACTAATGGGATTGGGATGGTGGGTGAGCTTACATCCATTGTCAGCTCTCTTTGGGAAGGGTTGGGGCTGCCATCGATAGGGCTAGGGCGAGTGCTTGAAGAAGAGGAAGAGCCATCTTGGGTCAATGACAGACGGGGGCTACTCCAATCTATTGGGGCCAACATCAAAGCGGATGTGGTAGTGGCTAAAGATGGAAGTGGAAAGTACAAGACAATAACGGAAGCCCTTCAAGAGGTCCCAAAGAAGAGCAATACAACCTTTATCATTTATATCAAGGAAGGAGTTTACGAAGAGCAAGTAATGGTCGACAAAAAAATGACCTATGTCATGATGATTGGAGATGGCCCGACTAAGACCAAGATCACTGCTAGAGAAAACTTTGTTGATGGAACATCCACATTCAAAACTGCTACTGTTTGTAAGTTTCCTATAATCATATTCATATAAATTCAGTTTATAATCATATTCATACAAATTCAACCAGCGAATTTAACATGATTTCATATTCTTAACAGCGGTGATTGGAACCAACTTCATCGGAAAAGACTTGTGGTTCGAGAACTCAGCTGGGGCTATAAAGCACCAAGCTGTTGCACTCCGAGTTCAATCTGACATGTCAATCTTCTATAACTGTCGAATGGACGGCTATCAAGATACTCTCTACACTCATGCCCACCGTCAATTCTATCGAGACTGCACCATCACTGGCACCATCGACTTCATTTTTGGCAACGCCGCCGTCGTGTTCCAAAATTGTAAGATCTTCGTGAGGAAGCCATTGGAAAATCAAAAATGCATTGTCACCGCCCAAGGTCGAACCGAGAAGAAAGAGCCTACCGGTATAGTCCTCCAAAACTGCGTCATCTCTTCCGACCCTGACTACTTTCCCGTCCGCCATATCAACAAGGCCTACCTTGGCCGCCCATGGAAGCAATATTCAAGAACCATTATCATGCAATCCCAAATCGATGACTTGATCCAGCCCGAAGGGTGGCTTCCATGGATGGACGATTTTGCTCTCAATACTCTATTTTACTCCGAGTTCGAAAATAGAGGACCCGGCGCAGCAACCCAAAATCGAGTCAAATGGAGGGGAGTTAAACAAATTACAGCAAAACACGCCCTTGATTTCACTCCTGGATTGTTTATTCGTGGTAATCCTTGGATCAAGCACACTGGAGTACCGTACACTTCCGGCATGATGGCAGTGTAA

mRNA sequence

ATGGCATGCAACAACAAGAAGGCTGCTCTGGTTGGCTTGTTATCTCTGGTCCTAGTTGCCATGGTTAACGGTGTGAGTGGCTCGGAAGAAGAGATTAAGACTCTGTGTGAACCTACTGATTACCGTGAAACTTGCGAGCAAGCCTTGGTGAATTCACATACTGACTCGGAAGACCCACGTGAGTTAATGAAGGCAGGATTCCATGCTGCCATTGAGAATCTCAAATCGGTTATTGGAAATTCCATCACTATGAAAGAAGCTTCCTCTGACCCTATGGTGAAGAAGGCACTTGATGATTGCAACGAGCTCATGGATTATGCAATTGATGATCTTTTATTATCGTATGATAAAATCACCGCTAATTTTGATTACAATAAAGTGAATGATTATACTGAGAATCTTAAGATTTGGTTGAGTGGTGCATTGACGTATCAAGATACTTGTTTGGACGGGTTTGAGAATGTTACTGGTGATACTGGTGAAAAGATGCAGAATTTGTTGAAGATACCGAAGGAAATGACCACTAATGGGATTGGGATGGTGGGTGAGCTTACATCCATTGTCAGCTCTCTTTGGGAAGGGTTGGGGCTGCCATCGATAGGGCTAGGGCGAGTGCTTGAAGAAGAGGAAGAGCCATCTTGGGTCAATGACAGACGGGGGCTACTCCAATCTATTGGGGCCAACATCAAAGCGGATGTGGTAGTGGCTAAAGATGGAAGTGGAAAGTACAAGACAATAACGGAAGCCCTTCAAGAGGTCCCAAAGAAGAGCAATACAACCTTTATCATTTATATCAAGGAAGGAGTTTACGAAGAGCAAGTAATGGTCGACAAAAAAATGACCTATGTCATGATGATTGGAGATGGCCCGACTAAGACCAAGATCACTGCTAGAGAAAACTTTGTTGATGGAACATCCACATTCAAAACTGCTACTGTTTCGGTGATTGGAACCAACTTCATCGGAAAAGACTTGTGGTTCGAGAACTCAGCTGGGGCTATAAAGCACCAAGCTGTTGCACTCCGAGTTCAATCTGACATGTCAATCTTCTATAACTGTCGAATGGACGGCTATCAAGATACTCTCTACACTCATGCCCACCGTCAATTCTATCGAGACTGCACCATCACTGGCACCATCGACTTCATTTTTGGCAACGCCGCCGTCGTGTTCCAAAATTGTAAGATCTTCGTGAGGAAGCCATTGGAAAATCAAAAATGCATTGTCACCGCCCAAGGTCGAACCGAGAAGAAAGAGCCTACCGGTATAGTCCTCCAAAACTGCGTCATCTCTTCCGACCCTGACTACTTTCCCGTCCGCCATATCAACAAGGCCTACCTTGGCCGCCCATGGAAGCAATATTCAAGAACCATTATCATGCAATCCCAAATCGATGACTTGATCCAGCCCGAAGGGTGGCTTCCATGGATGGACGATTTTGCTCTCAATACTCTATTTTACTCCGAGTTCGAAAATAGAGGACCCGGCGCAGCAACCCAAAATCGAGTCAAATGGAGGGGAGTTAAACAAATTACAGCAAAACACGCCCTTGATTTCACTCCTGGATTGTTTATTCGTGGTAATCCTTGGATCAAGCACACTGGAGTACCGTACACTTCCGGCATGATGGCAGTGTAA

Coding sequence (CDS)

ATGGCATGCAACAACAAGAAGGCTGCTCTGGTTGGCTTGTTATCTCTGGTCCTAGTTGCCATGGTTAACGGTGTGAGTGGCTCGGAAGAAGAGATTAAGACTCTGTGTGAACCTACTGATTACCGTGAAACTTGCGAGCAAGCCTTGGTGAATTCACATACTGACTCGGAAGACCCACGTGAGTTAATGAAGGCAGGATTCCATGCTGCCATTGAGAATCTCAAATCGGTTATTGGAAATTCCATCACTATGAAAGAAGCTTCCTCTGACCCTATGGTGAAGAAGGCACTTGATGATTGCAACGAGCTCATGGATTATGCAATTGATGATCTTTTATTATCGTATGATAAAATCACCGCTAATTTTGATTACAATAAAGTGAATGATTATACTGAGAATCTTAAGATTTGGTTGAGTGGTGCATTGACGTATCAAGATACTTGTTTGGACGGGTTTGAGAATGTTACTGGTGATACTGGTGAAAAGATGCAGAATTTGTTGAAGATACCGAAGGAAATGACCACTAATGGGATTGGGATGGTGGGTGAGCTTACATCCATTGTCAGCTCTCTTTGGGAAGGGTTGGGGCTGCCATCGATAGGGCTAGGGCGAGTGCTTGAAGAAGAGGAAGAGCCATCTTGGGTCAATGACAGACGGGGGCTACTCCAATCTATTGGGGCCAACATCAAAGCGGATGTGGTAGTGGCTAAAGATGGAAGTGGAAAGTACAAGACAATAACGGAAGCCCTTCAAGAGGTCCCAAAGAAGAGCAATACAACCTTTATCATTTATATCAAGGAAGGAGTTTACGAAGAGCAAGTAATGGTCGACAAAAAAATGACCTATGTCATGATGATTGGAGATGGCCCGACTAAGACCAAGATCACTGCTAGAGAAAACTTTGTTGATGGAACATCCACATTCAAAACTGCTACTGTTTCGGTGATTGGAACCAACTTCATCGGAAAAGACTTGTGGTTCGAGAACTCAGCTGGGGCTATAAAGCACCAAGCTGTTGCACTCCGAGTTCAATCTGACATGTCAATCTTCTATAACTGTCGAATGGACGGCTATCAAGATACTCTCTACACTCATGCCCACCGTCAATTCTATCGAGACTGCACCATCACTGGCACCATCGACTTCATTTTTGGCAACGCCGCCGTCGTGTTCCAAAATTGTAAGATCTTCGTGAGGAAGCCATTGGAAAATCAAAAATGCATTGTCACCGCCCAAGGTCGAACCGAGAAGAAAGAGCCTACCGGTATAGTCCTCCAAAACTGCGTCATCTCTTCCGACCCTGACTACTTTCCCGTCCGCCATATCAACAAGGCCTACCTTGGCCGCCCATGGAAGCAATATTCAAGAACCATTATCATGCAATCCCAAATCGATGACTTGATCCAGCCCGAAGGGTGGCTTCCATGGATGGACGATTTTGCTCTCAATACTCTATTTTACTCCGAGTTCGAAAATAGAGGACCCGGCGCAGCAACCCAAAATCGAGTCAAATGGAGGGGAGTTAAACAAATTACAGCAAAACACGCCCTTGATTTCACTCCTGGATTGTTTATTCGTGGTAATCCTTGGATCAAGCACACTGGAGTACCGTACACTTCCGGCATGATGGCAGTGTAA

Protein sequence

MACNNKKAALVGLLSLVLVAMVNGVSGSEEEIKTLCEPTDYRETCEQALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELMDYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGIGMVGELTSIVSSLWEGLGLPSIGLGRVLEEEEEPSWVNDRRGLLQSIGANIKADVVVAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHTGVPYTSGMMAV

Homology

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BLAST of CmoCh06G015160 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 575.9 bits (1483), Expect = 5.0e-163
Identity = 268/523 (51.24%), Postives = 378/523 (72.28%), Query Frame = 0


Query: 25  VSGSEEEIKTLCEPTDYRETCEQALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNSITM 84
           V+ S + +K +C PTDYR+TCE  L+ +  ++ DP EL+K  F+  ++ +      S T+
Sbjct: 51  VNASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTI 110

Query: 85  KEASSDPMVKKALDDCNELMDYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTY 144
            E   D   + ALD C ELMDYA+D+L  S++++   F+++ +++   NL+IWLS A+++
Sbjct: 111 MELQKDSRTRMALDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISH 170

Query: 145 QDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGIGMVGELTSIVSSLWEGLGLPSIGLGR 204
           ++TCL+GF+   G+ GE M+  LK   E+T NG+ ++ E+++ V      + +P +   R
Sbjct: 171 EETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQ----MQIPGLNSRR 230

Query: 205 VLEEEEEPSWVNDR-RGLLQSIGA--NIKADVVVAKDGSGKYKTITEALQEVPKKSNTTF 264
           +L  E  PSWV+ R R LLQ+  A  ++K D+VVA+DGSG+YKTI EALQ VPKK NTTF
Sbjct: 231 LL-AEGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTF 290

Query: 265 IIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITARENFVDGTSTFKTATVSVIGTNFI 324
           +++IK G+Y+E V V+K M++++ IGDGP KT I+  +N+ DG +T++TATV+++G  FI
Sbjct: 291 VVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFI 350

Query: 325 GKDLWFENSAGAIKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTID 384
            K++ FEN+AGAIKHQAVA+RVQSD SIF+NCR DGYQDTLYTH+HRQF+RDCTI+GTID
Sbjct: 351 AKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTID 410

Query: 385 FIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRH 444
           F+FG+AA VFQNC + VRKPL NQ C +TA GR + +E TG V Q C I+ +PDY  V+ 
Sbjct: 411 FLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKE 470

Query: 445 INKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQN 504
            +KAYLGRPWK+YSRTIIM + I D +QP+GW PW+ DF L TLFYSE +N GPG+A  N
Sbjct: 471 TSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALAN 530

Query: 505 RVKWRGVKQITAKHALDFTPGLFIRGNPWIKHTGVPYTSGMMA 545
           RV W G+K ++ +  L FTP  +I+G+ WI   GVPYT+G++A
Sbjct: 531 RVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLA 567

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BLAST of CmoCh06G015160 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.7e-161
Identity = 276/561 (49.20%), Postives = 385/561 (68.63%), Query Frame = 0


Query: 2   ACNNKKAALVGLLSLVLVAMVNGV-----------SGSEE------EIKTLCEPTDYRET 61
           A   K+  ++ + S++L++MV  V           +G EE       IK +C PTDY+ET
Sbjct: 10  AKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKET 69

Query: 62  CEQALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELM 121
           CE  L     D+ DP EL+K  F+A ++ +  V   S TM E   DP  K ALD C ELM
Sbjct: 70  CEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELM 129

Query: 122 DYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQ 181
           DYAI +L  S++++   F+++KV++    L+IWLS  ++++ TCLDGF+   G+ GE ++
Sbjct: 130 DYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIK 189

Query: 182 NLLKIPKEMTTNGIGMVGELTSIVSSLWEGLGLPSIGLGRVLEEEEEPSWVNDR-RGLLQ 241
             LK   ++T NG+ MV E+++ +      + +P +   R+L  +E PSW++ R R LL 
Sbjct: 190 KALKTAVQLTHNGLAMVTEMSNYLGQ----MQIPEMNSRRLL-SQEFPSWMDARARRLLN 249

Query: 242 SIGANIKADVVVAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYV 301
           +  + +K D+VVA+DGSG+YKTI EAL  VPKK NTTF+++IKEG+Y+E V V++ MT++
Sbjct: 250 APMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHL 309

Query: 302 MMIGDGPTKTKITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRV 361
           + IGDGP KT I+  +++ DG +T+KTATV+++G +FI K++ FEN+AGAIKHQAVA+RV
Sbjct: 310 VFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRV 369

Query: 362 QSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLE 421
            +D SIFYNC+ DGYQDTLY H+HRQFYRDCTI+GTIDF+FG+AA VFQNC + VRKPL 
Sbjct: 370 LADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLL 429

Query: 422 NQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQ 481
           NQ C +TA GR + +E TG VLQ C I  +PDY  V+  +K YLGRPWK+YSRTIIM + 
Sbjct: 430 NQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTF 489

Query: 482 IDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGL 541
           I D + PEGW PW+ +F LNTLFYSE +N GPGAA   RV W G+K+++ +  L FTP  
Sbjct: 490 IPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQ 549

Query: 542 FIRGNPWIKHTGVPYTSGMMA 545
           +I+G+ WI   GVPY  G+ +
Sbjct: 550 YIQGDAWIPGKGVPYILGLFS 564

[Close]

BLAST of CmoCh06G015160 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 3.3e-151
Identity = 273/561 (48.66%), Postives = 376/561 (67.02%), Query Frame = 0


Query: 6   KKAALVGLLSLVLVAMVNGV--------SGSEE----EIKT-------LCEPTDYRETCE 65
           KK  + G+++ +LV MV  V        S SE+    +IKT       +C PTDY+ETC 
Sbjct: 10  KKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCV 69

Query: 66  QALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNS---ITMKEASSDPMVKKALDDCNEL 125
            +L+ +  DS  P +L+K GF+  I +++  I  +   +T K A++D   K AL+ C +L
Sbjct: 70  NSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAK-AANDKDTKGALELCEKL 129

Query: 126 MDYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKM 185
           M+ A DDL    D     F   ++ D+ E+L++WLSG++ YQ TC+D FE       + M
Sbjct: 130 MNDATDDLKKCLDNFD-GFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDM 189

Query: 186 QNLLKIPKEMTTNGIGMVGELTSI-----VSSLWEGLGLPSIGLGRVLEEEEEPSWVNDR 245
           Q + K  +E+T+NG+ M+  ++++     V+ +   LG  +  L  +  E+  PSWV   
Sbjct: 190 QKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKL--LSAEDGIPSWVGPN 249

Query: 246 RGLLQSIGANIKADVVVAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDK 305
              L +    +KA+VVVA DGSG+YKTI EAL  VPK +   F+IYIK+GVY E+V V K
Sbjct: 250 TRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTK 309

Query: 306 KMTYVMMIGDGPTKTKITARENFVDG-TSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQ 365
           KMT+V  IGDGPTKTKIT   N+  G   T+ TATV++ G NF  K++ FEN+AG   HQ
Sbjct: 310 KMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQ 369

Query: 366 AVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIF 425
           AVALRV +D+++FYNC++DGYQDTLY H+HRQF+RDCT++GT+DFIFG+  VV QNC I 
Sbjct: 370 AVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIV 429

Query: 426 VRKPLENQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRT 485
           VRKP+++Q C++TAQGR++K+E TG+VLQNC I+ +P Y PV+ INKAYLGRPWK++SRT
Sbjct: 430 VRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRT 489

Query: 486 IIMQSQIDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHAL 539
           IIM + IDD+I P GWLPW  DFALNTL+Y+E+EN GPG+    RVKW G+K+++ K AL
Sbjct: 490 IIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQAL 549

[Close]

BLAST of CmoCh06G015160 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 481.1 bits (1237), Expect = 1.7e-134
Identity = 243/561 (43.32%), Postives = 361/561 (64.35%), Query Frame = 0


Query: 6   KKAALVGLLSLVLVAMV----------------NGVSGSEEEIKTLCEPTDYRETCEQAL 65
           KK  + G +S  LV MV                N +  + + ++ +C PTD+++TC  +L
Sbjct: 9   KKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTCVNSL 68

Query: 66  VNSHTDSEDPRELMKAGFHAAIENLKSVI--GNSITMKEASSDPMVKKALDDCNELMDYA 125
           + +  DS+DP +L+K GF   I+++   +   +     +A  +P  K A + C +LM  A
Sbjct: 69  MGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKLMIDA 128

Query: 126 IDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLL 185
           IDDL    D     F  +++  + E+L++WLSG++ +Q TC+D F  +  +  + M  + 
Sbjct: 129 IDDLKKCMDH---GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDMLKIF 188

Query: 186 KIPKEMTTNGIGMVGELTSIV-SSLWEGL--GLPSIGLGRVLEEEEEPSWVN-DRRGLLQ 245
           K  +E+++N + MV  +++++ +S   GL   L       +  E+  P+WV  + R L+ 
Sbjct: 189 KTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWVGPEARRLMA 248

Query: 246 SIGAN---IKADVVVAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKM 305
           + G     +KA+ VVA+DG+G++KTIT+AL  VPK +   FII+IKEG+Y+E+V V KKM
Sbjct: 249 AQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEKVTVTKKM 308

Query: 306 TYVMMIGDGPTKTKITARENF-VDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAV 365
            +V  IGDGP KT IT   NF +    TF TAT+++ G +F  K++  EN+AG    QAV
Sbjct: 309 PHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAGPEGGQAV 368

Query: 366 ALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVR 425
           ALRV +D ++F++C++DG+QDTLY H+HRQFYRDCT++GT+DFIFG+A  + QNCKI VR
Sbjct: 369 ALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVR 428

Query: 426 KPLENQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTII 485
           KP + Q C+VTAQGR+  +E TG+VL  C I+ DP Y P++ +NKAYLGRPWK++SRTII
Sbjct: 429 KPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRPWKEFSRTII 488

Query: 486 MQSQIDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDF 541
           M++ IDD+I P GWLPW  DFAL TL+Y+E  N GPG+    RVKW G+K++T + AL +
Sbjct: 489 MKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDALLY 548

[Close]

BLAST of CmoCh06G015160 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-127
Identity = 254/656 (38.72%), Postives = 359/656 (54.73%), Query Frame = 0


Query: 32  IKTLCEPTDYRETCEQALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNSI--TMKEASS 91
           +  +C  TDY++ C  +L      S DPR L+++ F  AI +++S I   +      A +
Sbjct: 48  VSIICASTDYKQDCTTSLAT--VRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADA 107

Query: 92  DPMVKKALDDCNELMDYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCL 151
           D   ++AL+ C ELMD AIDDL  + DK    F + +++D+ E+L +WLSG++TYQ TC+
Sbjct: 108 DMHTREALNTCRELMDDAIDDLRKTRDKF-RGFLFTRLSDFVEDLCVWLSGSITYQQTCI 167

Query: 152 DGFENVTGDTGEKMQNLLKIPKEMTTNGIGMVGELTSIV--------------------- 211
           DGFE +  +    M+ +++  + +T+NG+ +   L  ++                     
Sbjct: 168 DGFEGIDSEAAVMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILG 227

Query: 212 --SSLWEGLG-------------------------------------------------- 271
             SS  E +G                                                  
Sbjct: 228 SGSSRDESVGSSQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQ 287

Query: 272 ------------------------------------------------------------ 331
                                                                       
Sbjct: 288 QMESSEDTPKKSAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPL 347

Query: 332 --------LPSIGLGRVLEEEEEPSWV--NDRRGLLQSIGAN-IKADVVVAKDGSGKYKT 391
                   L S+    + EE E P WV  + RR L +    N IKA+VVVAKDGSGK KT
Sbjct: 348 RKLNPLNKLDSLKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKT 407

Query: 392 ITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITARENFV-DG 451
           I +AL  VP K+   F+I+IKEGVY+E+V V KKM +VM +GDGPTKT IT    F+ D 
Sbjct: 408 IAQALAMVPMKNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQ 467

Query: 452 TSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCRMDGYQDTLYT 511
             T++TA+V+V G  F+ KD+ FEN+AGA +HQAVALRV +D ++F+NC M+GYQDTLY 
Sbjct: 468 VGTYRTASVAVNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYV 527

Query: 512 HAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGRTEKKEPTGIV 541
           H HRQFYR+C ++GTIDF+FG+A  VFQNC+  +R+P+E+Q+CIVTAQGR +++E TGIV
Sbjct: 528 HTHRQFYRNCRVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIV 587

[Close]

BLAST of CmoCh06G015160 vs. ExPASy TrEMBL
Match: A0A6J1ET06 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437278 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0


Query: 1   MACNNKKAALVGLLSLVLVAMVNGVSGSEEEIKTLCEPTDYRETCEQALVNSHTDSEDPR 60
           MACNNKKAALVGLLSLVLVAMVNGVSGSEEEIKTLCEPTDYRETCEQALVNSHTDSEDPR
Sbjct: 1   MACNNKKAALVGLLSLVLVAMVNGVSGSEEEIKTLCEPTDYRETCEQALVNSHTDSEDPR 60

Query: 61  ELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELMDYAIDDLLLSYDKITA 120
           ELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELMDYAIDDLLLSYDKITA
Sbjct: 61  ELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELMDYAIDDLLLSYDKITA 120

Query: 121 NFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGIGM 180
           NFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGIGM
Sbjct: 121 NFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGIGM 180

Query: 181 VGELTSIVSSLWEGLGLPSIGLGRVLEEEEEPSWVNDRRGLLQSIGANIKADVVVAKDGS 240
           VGELTSIVSSLWEGLGLPSIGLGRVLEEEEEPSWVNDRRGLLQSIGANIKADVVVAKDGS
Sbjct: 181 VGELTSIVSSLWEGLGLPSIGLGRVLEEEEEPSWVNDRRGLLQSIGANIKADVVVAKDGS 240

Query: 241 GKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITAREN 300
           GKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITAREN
Sbjct: 241 GKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITAREN 300

Query: 301 FVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCRMDGYQD 360
           FVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCRMDGYQD
Sbjct: 301 FVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCRMDGYQD 360

Query: 361 TLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGRTEKKEP 420
           TLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGRTEKKEP
Sbjct: 361 TLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGRTEKKEP 420

Query: 421 TGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWMDDF 480
           TGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWMDDF
Sbjct: 421 TGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWMDDF 480

Query: 481 ALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHTGVPYTS 540
           ALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHTGVPYTS
Sbjct: 481 ALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHTGVPYTS 540

Query: 541 GMMAV 546
           GMMAV
Sbjct: 541 GMMAV 545

[Close]

BLAST of CmoCh06G015160 vs. ExPASy TrEMBL
Match: A0A6J1IBZ7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472271 PE=3 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 2.3e-304
Identity = 520/551 (94.37%), Postives = 532/551 (96.55%), Query Frame = 0


Query: 1   MACNNKKAALVGLLSLVLVAMVNGVSGSEE------EIKTLCEPTDYRETCEQALVNSHT 60
           MAC NKKAALVGLL LVLVAMVN VSGSEE      EIKTLCEPTDYRETCEQALVNSHT
Sbjct: 1   MAC-NKKAALVGLLYLVLVAMVNVVSGSEEESTSQKEIKTLCEPTDYRETCEQALVNSHT 60

Query: 61  DSEDPRELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELMDYAIDDLLLS 120
           DSEDPRELMKAGFHAAIE+LKSVIGNS TMKEA+SDP+VKKALDDCNELMDYAIDDLLLS
Sbjct: 61  DSEDPRELMKAGFHAAIEHLKSVIGNSTTMKEAASDPLVKKALDDCNELMDYAIDDLLLS 120

Query: 121 YDKITANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLLKIPKEMT 180
           +DKIT NFDYNK+NDYTENLKIWLSGALTYQ+TCLDGFENVTGDTGEKMQNLLKI KEMT
Sbjct: 121 FDKITDNFDYNKMNDYTENLKIWLSGALTYQETCLDGFENVTGDTGEKMQNLLKISKEMT 180

Query: 181 TNGIGMVGELTSIVSSLWEGLGLPSIGLGRVLEEEEEPSWVNDRRGLLQSIGANIKADVV 240
            NGIGMVGELTSIV+SLWEGLGLPSIGLGRVL+EEEEPSWVNDRRGLLQS G NIKA+VV
Sbjct: 181 ANGIGMVGELTSIVTSLWEGLGLPSIGLGRVLKEEEEPSWVNDRRGLLQSTGVNIKANVV 240

Query: 241 VAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK 300
           VAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK
Sbjct: 241 VAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK 300

Query: 301 ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCR 360
           ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGA KHQAVALRVQSDMSIFYNCR
Sbjct: 301 ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIFYNCR 360

Query: 361 MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGR 420
           MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKP++NQKCIVTAQGR
Sbjct: 361 MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPMDNQKCIVTAQGR 420

Query: 421 TEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWL 480
           TEKKEPTGI+LQNCVISSDPDYFPVRH NKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWL
Sbjct: 421 TEKKEPTGIILQNCVISSDPDYFPVRHTNKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWL 480

Query: 481 PWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHT 540
           PW  DFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHT
Sbjct: 481 PWTGDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHT 540

Query: 541 GVPYTSGMMAV 546
           GVPYTSGMMAV
Sbjct: 541 GVPYTSGMMAV 550

[Close]

BLAST of CmoCh06G015160 vs. ExPASy TrEMBL
Match: A0A6J1IE10 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472305 PE=3 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.5e-271
Identity = 458/549 (83.42%), Postives = 500/549 (91.07%), Query Frame = 0


Query: 5   NKKAALVGLLSLVLVAMVNGVSGSEEE------IKTLCEPTDYRETCEQALVNSHTDSED 64
           NK AALVGLLSL +VAMVNGV G+EE       IKTLCEPTDYR+TCE+ALV S TDS+D
Sbjct: 11  NKVAALVGLLSLFVVAMVNGVHGAEEHSESSSAIKTLCEPTDYRKTCEEALVKSKTDSQD 70

Query: 65  PRELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELMDYAIDDLLLSYDKI 124
           PREL+KAGFH  +E +KS I NS T+KE ++DPM KKALDDC+ELMDYAIDDLLLS+DKI
Sbjct: 71  PRELIKAGFHYVVEQIKSAIKNSTTLKETANDPMAKKALDDCSELMDYAIDDLLLSFDKI 130

Query: 125 TANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGI 184
           T  FD++K++DY E+L+IWLSGALTYQ TCLDGFENVTGDTGEKM++LLK+ +EMT N +
Sbjct: 131 TETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKLSQEMTANAL 190

Query: 185 GMVGELTSIVSSLWEGLGLPSIG----LGRVLEEEEEPSWVNDRRGLLQSIGANIKADVV 244
            MVGE+ S+VSSLWEGLGLP +G         EEEE PSWVNDRRGLLQ+ GAN+KADVV
Sbjct: 191 EMVGEVKSVVSSLWEGLGLPPLGRELRTAESKEEEELPSWVNDRRGLLQAKGANVKADVV 250

Query: 245 VAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK 304
           VAKDGSGKYKTIT+ALQ+VPKKSN TF+IYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK
Sbjct: 251 VAKDGSGKYKTITQALQDVPKKSNKTFVIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK 310

Query: 305 ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCR 364
           ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGA KHQAVALRVQSDMSIFYNCR
Sbjct: 311 ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIFYNCR 370

Query: 365 MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGR 424
           MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPL+NQKCIVTAQGR
Sbjct: 371 MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLDNQKCIVTAQGR 430

Query: 425 TEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWL 484
           T+KKEPT I+LQNCVISSDPDYFPVRHINKAYLGRPWK YSRTIIM+SQIDDLIQPEGWL
Sbjct: 431 TQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKAYSRTIIMESQIDDLIQPEGWL 490

Query: 485 PWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHT 544
           PWM +FALNTLFYSEF NRGPGA+T++RVKW+G+KQITAKHALDFTP LFIRGNPWIK T
Sbjct: 491 PWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHALDFTPALFIRGNPWIKPT 550

[Close]

BLAST of CmoCh06G015160 vs. ExPASy TrEMBL
Match: A0A6J1I9B3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472272 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 455/549 (82.88%), Postives = 501/549 (91.26%), Query Frame = 0


Query: 5   NKKAALVGLLSLVLVAMVNGVSGSEEE------IKTLCEPTDYRETCEQALVNSHTDSED 64
           NK AALVGL SL++VAM+NGV+G+E+       IKTLCEPTDYR+TCE+ALV S TDS+D
Sbjct: 12  NKVAALVGLSSLLVVAMLNGVNGAEDHSESSSTIKTLCEPTDYRQTCEEALVKSKTDSQD 71

Query: 65  PRELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELMDYAIDDLLLSYDKI 124
           PREL+KAGFH  +E +KS I NS T+KE ++DPM KKALDDC+ELMDYAIDDLLLS+DKI
Sbjct: 72  PRELIKAGFHYVVEQIKSAIKNSTTLKETANDPMAKKALDDCSELMDYAIDDLLLSFDKI 131

Query: 125 TANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGI 184
           T  FD++K++DY E+L+IWLSGALTYQ TCLDGFENVTGDTGEKM++LLK+ +EMT N +
Sbjct: 132 TETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKLSQEMTANAL 191

Query: 185 GMVGELTSIVSSLWEGLGLPSIG----LGRVLEEEEEPSWVNDRRGLLQSIGANIKADVV 244
            MVGE+ S+VSSLWEGLGLP +G         EEEE PSWVNDRRGLLQ+ GAN+KADVV
Sbjct: 192 EMVGEVKSVVSSLWEGLGLPPLGRELRTAESKEEEELPSWVNDRRGLLQAKGANVKADVV 251

Query: 245 VAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK 304
           VAKDGSGKYKTIT+ALQ+VPKKSN TF+IYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK
Sbjct: 252 VAKDGSGKYKTITQALQDVPKKSNKTFVIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK 311

Query: 305 ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCR 364
           ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGA KHQAVALRVQSDMSIFYNCR
Sbjct: 312 ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIFYNCR 371

Query: 365 MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGR 424
           MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGR
Sbjct: 372 MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGR 431

Query: 425 TEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWL 484
           T+KKEPT I+LQNCVISSDPDYFPVRHINKAYLGRPWK YSRTIIM+SQIDDLIQPEGWL
Sbjct: 432 TQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKAYSRTIIMESQIDDLIQPEGWL 491

Query: 485 PWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHT 544
           PWM +FALNTLFYSEF NRGPGA+T++RVKW+G+KQITAKHALDFTP LFIRGNPWIK T
Sbjct: 492 PWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHALDFTPALFIRGNPWIKPT 551

[Close]

BLAST of CmoCh06G015160 vs. ExPASy TrEMBL
Match: A0A6J1G2K0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450212 PE=3 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 4.8e-270
Identity = 458/549 (83.42%), Postives = 493/549 (89.80%), Query Frame = 0


Query: 5   NKKAALVGLLSLVLVAMVNGVSGSEEE------IKTLCEPTDYRETCEQALVNSHTDSED 64
           NK AALVGLLSL +VAMV GV+G+EE       IKTLCEPTDYR+TCE+AL  S TDSED
Sbjct: 11  NKTAALVGLLSLFVVAMVTGVNGAEEHSESSGAIKTLCEPTDYRQTCEEALAKSKTDSED 70

Query: 65  PRELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELMDYAIDDLLLSYDKI 124
           PREL+KA FH  +E +KS I NS T+KE + DPM KKALDDC+ELMDYAIDDLLLS+DKI
Sbjct: 71  PRELIKASFHYVVEQIKSAIKNSTTLKETAKDPMAKKALDDCSELMDYAIDDLLLSFDKI 130

Query: 125 TANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGI 184
           T  FD++K++DY E+L+IWLSGALTYQ TCLDGFENVTGDTGEKM++LLK+ +EMT N I
Sbjct: 131 TETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKMSQEMTANAI 190

Query: 185 GMVGELTSIVSSLWEGLGLPSIG----LGRVLEEEEEPSWVNDRRGLLQSIGANIKADVV 244
            MVGE+ S VSSLWE  GLP +G         EEEE PSWVNDRRGLLQ+ GAN+KADVV
Sbjct: 191 EMVGEVKSAVSSLWESFGLPPLGRELRTAESKEEEEVPSWVNDRRGLLQTTGANVKADVV 250

Query: 245 VAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTK 304
           VAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQV VDKKMTYVMMIGDGPTKTK
Sbjct: 251 VAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVKVDKKMTYVMMIGDGPTKTK 310

Query: 305 ITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCR 364
           ITARENF DGTSTFKTATVSVIGTNFIGKDLWFENSAGA KHQAVALRVQSDMSIFYNCR
Sbjct: 311 ITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIFYNCR 370

Query: 365 MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGR 424
           MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVV QNCKIFVRKPLENQKCIVTAQGR
Sbjct: 371 MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIFVRKPLENQKCIVTAQGR 430

Query: 425 TEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWL 484
           T+KKEPT I+LQNCVISSDPDYFPVRHINKAYLGRPWK+YSRTIIM+SQIDDLIQPEGWL
Sbjct: 431 TQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRTIIMESQIDDLIQPEGWL 490

Query: 485 PWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGLFIRGNPWIKHT 544
           PWM +FALNTLFYSEF NRGPGA+T+NRVKWRG+KQITAKHALDFTP LFIRGNPWIK T
Sbjct: 491 PWMGNFALNTLFYSEFNNRGPGASTKNRVKWRGIKQITAKHALDFTPALFIRGNPWIKPT 550

[Close]

BLAST of CmoCh06G015160 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 575.9 bits (1483), Expect = 3.5e-164
Identity = 268/523 (51.24%), Postives = 378/523 (72.28%), Query Frame = 0


Query: 25  VSGSEEEIKTLCEPTDYRETCEQALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNSITM 84
           V+ S + +K +C PTDYR+TCE  L+ +  ++ DP EL+K  F+  ++ +      S T+
Sbjct: 51  VNASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTI 110

Query: 85  KEASSDPMVKKALDDCNELMDYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTY 144
            E   D   + ALD C ELMDYA+D+L  S++++   F+++ +++   NL+IWLS A+++
Sbjct: 111 MELQKDSRTRMALDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISH 170

Query: 145 QDTCLDGFENVTGDTGEKMQNLLKIPKEMTTNGIGMVGELTSIVSSLWEGLGLPSIGLGR 204
           ++TCL+GF+   G+ GE M+  LK   E+T NG+ ++ E+++ V      + +P +   R
Sbjct: 171 EETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQ----MQIPGLNSRR 230

Query: 205 VLEEEEEPSWVNDR-RGLLQSIGA--NIKADVVVAKDGSGKYKTITEALQEVPKKSNTTF 264
           +L  E  PSWV+ R R LLQ+  A  ++K D+VVA+DGSG+YKTI EALQ VPKK NTTF
Sbjct: 231 LL-AEGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTF 290

Query: 265 IIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITARENFVDGTSTFKTATVSVIGTNFI 324
           +++IK G+Y+E V V+K M++++ IGDGP KT I+  +N+ DG +T++TATV+++G  FI
Sbjct: 291 VVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFI 350

Query: 325 GKDLWFENSAGAIKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTID 384
            K++ FEN+AGAIKHQAVA+RVQSD SIF+NCR DGYQDTLYTH+HRQF+RDCTI+GTID
Sbjct: 351 AKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTID 410

Query: 385 FIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRH 444
           F+FG+AA VFQNC + VRKPL NQ C +TA GR + +E TG V Q C I+ +PDY  V+ 
Sbjct: 411 FLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKE 470

Query: 445 INKAYLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQN 504
            +KAYLGRPWK+YSRTIIM + I D +QP+GW PW+ DF L TLFYSE +N GPG+A  N
Sbjct: 471 TSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALAN 530

Query: 505 RVKWRGVKQITAKHALDFTPGLFIRGNPWIKHTGVPYTSGMMA 545
           RV W G+K ++ +  L FTP  +I+G+ WI   GVPYT+G++A
Sbjct: 531 RVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLA 567

[Close]

BLAST of CmoCh06G015160 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 570.1 bits (1468), Expect = 1.9e-162
Identity = 276/561 (49.20%), Postives = 385/561 (68.63%), Query Frame = 0


Query: 2   ACNNKKAALVGLLSLVLVAMVNGV-----------SGSEE------EIKTLCEPTDYRET 61
           A   K+  ++ + S++L++MV  V           +G EE       IK +C PTDY+ET
Sbjct: 10  AKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKET 69

Query: 62  CEQALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNSITMKEASSDPMVKKALDDCNELM 121
           CE  L     D+ DP EL+K  F+A ++ +  V   S TM E   DP  K ALD C ELM
Sbjct: 70  CEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELM 129

Query: 122 DYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQ 181
           DYAI +L  S++++   F+++KV++    L+IWLS  ++++ TCLDGF+   G+ GE ++
Sbjct: 130 DYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIK 189

Query: 182 NLLKIPKEMTTNGIGMVGELTSIVSSLWEGLGLPSIGLGRVLEEEEEPSWVNDR-RGLLQ 241
             LK   ++T NG+ MV E+++ +      + +P +   R+L  +E PSW++ R R LL 
Sbjct: 190 KALKTAVQLTHNGLAMVTEMSNYLGQ----MQIPEMNSRRLL-SQEFPSWMDARARRLLN 249

Query: 242 SIGANIKADVVVAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYV 301
           +  + +K D+VVA+DGSG+YKTI EAL  VPKK NTTF+++IKEG+Y+E V V++ MT++
Sbjct: 250 APMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHL 309

Query: 302 MMIGDGPTKTKITARENFVDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRV 361
           + IGDGP KT I+  +++ DG +T+KTATV+++G +FI K++ FEN+AGAIKHQAVA+RV
Sbjct: 310 VFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRV 369

Query: 362 QSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLE 421
            +D SIFYNC+ DGYQDTLY H+HRQFYRDCTI+GTIDF+FG+AA VFQNC + VRKPL 
Sbjct: 370 LADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLL 429

Query: 422 NQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTIIMQSQ 481
           NQ C +TA GR + +E TG VLQ C I  +PDY  V+  +K YLGRPWK+YSRTIIM + 
Sbjct: 430 NQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTF 489

Query: 482 IDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDFTPGL 541
           I D + PEGW PW+ +F LNTLFYSE +N GPGAA   RV W G+K+++ +  L FTP  
Sbjct: 490 IPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQ 549

Query: 542 FIRGNPWIKHTGVPYTSGMMA 545
           +I+G+ WI   GVPY  G+ +
Sbjct: 550 YIQGDAWIPGKGVPYILGLFS 564

[Close]

BLAST of CmoCh06G015160 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 536.6 bits (1381), Expect = 2.4e-152
Identity = 273/561 (48.66%), Postives = 376/561 (67.02%), Query Frame = 0


Query: 6   KKAALVGLLSLVLVAMVNGV--------SGSEE----EIKT-------LCEPTDYRETCE 65
           KK  + G+++ +LV MV  V        S SE+    +IKT       +C PTDY+ETC 
Sbjct: 10  KKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCV 69

Query: 66  QALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNS---ITMKEASSDPMVKKALDDCNEL 125
            +L+ +  DS  P +L+K GF+  I +++  I  +   +T K A++D   K AL+ C +L
Sbjct: 70  NSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAK-AANDKDTKGALELCEKL 129

Query: 126 MDYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKM 185
           M+ A DDL    D     F   ++ D+ E+L++WLSG++ YQ TC+D FE       + M
Sbjct: 130 MNDATDDLKKCLDNFD-GFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDM 189

Query: 186 QNLLKIPKEMTTNGIGMVGELTSI-----VSSLWEGLGLPSIGLGRVLEEEEEPSWVNDR 245
           Q + K  +E+T+NG+ M+  ++++     V+ +   LG  +  L  +  E+  PSWV   
Sbjct: 190 QKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKL--LSAEDGIPSWVGPN 249

Query: 246 RGLLQSIGANIKADVVVAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDK 305
              L +    +KA+VVVA DGSG+YKTI EAL  VPK +   F+IYIK+GVY E+V V K
Sbjct: 250 TRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTK 309

Query: 306 KMTYVMMIGDGPTKTKITARENFVDG-TSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQ 365
           KMT+V  IGDGPTKTKIT   N+  G   T+ TATV++ G NF  K++ FEN+AG   HQ
Sbjct: 310 KMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQ 369

Query: 366 AVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIF 425
           AVALRV +D+++FYNC++DGYQDTLY H+HRQF+RDCT++GT+DFIFG+  VV QNC I 
Sbjct: 370 AVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIV 429

Query: 426 VRKPLENQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRT 485
           VRKP+++Q C++TAQGR++K+E TG+VLQNC I+ +P Y PV+ INKAYLGRPWK++SRT
Sbjct: 430 VRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRT 489

Query: 486 IIMQSQIDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHAL 539
           IIM + IDD+I P GWLPW  DFALNTL+Y+E+EN GPG+    RVKW G+K+++ K AL
Sbjct: 490 IIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQAL 549

[Close]

BLAST of CmoCh06G015160 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 481.1 bits (1237), Expect = 1.2e-135
Identity = 243/561 (43.32%), Postives = 361/561 (64.35%), Query Frame = 0


Query: 6   KKAALVGLLSLVLVAMV----------------NGVSGSEEEIKTLCEPTDYRETCEQAL 65
           KK  + G +S  LV MV                N +  + + ++ +C PTD+++TC  +L
Sbjct: 9   KKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTCVNSL 68

Query: 66  VNSHTDSEDPRELMKAGFHAAIENLKSVI--GNSITMKEASSDPMVKKALDDCNELMDYA 125
           + +  DS+DP +L+K GF   I+++   +   +     +A  +P  K A + C +LM  A
Sbjct: 69  MGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKLMIDA 128

Query: 126 IDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCLDGFENVTGDTGEKMQNLL 185
           IDDL    D     F  +++  + E+L++WLSG++ +Q TC+D F  +  +  + M  + 
Sbjct: 129 IDDLKKCMDH---GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDMLKIF 188

Query: 186 KIPKEMTTNGIGMVGELTSIV-SSLWEGL--GLPSIGLGRVLEEEEEPSWVN-DRRGLLQ 245
           K  +E+++N + MV  +++++ +S   GL   L       +  E+  P+WV  + R L+ 
Sbjct: 189 KTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWVGPEARRLMA 248

Query: 246 SIGAN---IKADVVVAKDGSGKYKTITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKM 305
           + G     +KA+ VVA+DG+G++KTIT+AL  VPK +   FII+IKEG+Y+E+V V KKM
Sbjct: 249 AQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEKVTVTKKM 308

Query: 306 TYVMMIGDGPTKTKITARENF-VDGTSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAV 365
            +V  IGDGP KT IT   NF +    TF TAT+++ G +F  K++  EN+AG    QAV
Sbjct: 309 PHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAGPEGGQAV 368

Query: 366 ALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVR 425
           ALRV +D ++F++C++DG+QDTLY H+HRQFYRDCT++GT+DFIFG+A  + QNCKI VR
Sbjct: 369 ALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVR 428

Query: 426 KPLENQKCIVTAQGRTEKKEPTGIVLQNCVISSDPDYFPVRHINKAYLGRPWKQYSRTII 485
           KP + Q C+VTAQGR+  +E TG+VL  C I+ DP Y P++ +NKAYLGRPWK++SRTII
Sbjct: 429 KPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRPWKEFSRTII 488

Query: 486 MQSQIDDLIQPEGWLPWMDDFALNTLFYSEFENRGPGAATQNRVKWRGVKQITAKHALDF 541
           M++ IDD+I P GWLPW  DFAL TL+Y+E  N GPG+    RVKW G+K++T + AL +
Sbjct: 489 MKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDALLY 548

[Close]

BLAST of CmoCh06G015160 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 458.4 bits (1178), Expect = 8.2e-129
Identity = 254/656 (38.72%), Postives = 359/656 (54.73%), Query Frame = 0


Query: 32  IKTLCEPTDYRETCEQALVNSHTDSEDPRELMKAGFHAAIENLKSVIGNSI--TMKEASS 91
           +  +C  TDY++ C  +L      S DPR L+++ F  AI +++S I   +      A +
Sbjct: 48  VSIICASTDYKQDCTTSLAT--VRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADA 107

Query: 92  DPMVKKALDDCNELMDYAIDDLLLSYDKITANFDYNKVNDYTENLKIWLSGALTYQDTCL 151
           D   ++AL+ C ELMD AIDDL  + DK    F + +++D+ E+L +WLSG++TYQ TC+
Sbjct: 108 DMHTREALNTCRELMDDAIDDLRKTRDKF-RGFLFTRLSDFVEDLCVWLSGSITYQQTCI 167

Query: 152 DGFENVTGDTGEKMQNLLKIPKEMTTNGIGMVGELTSIV--------------------- 211
           DGFE +  +    M+ +++  + +T+NG+ +   L  ++                     
Sbjct: 168 DGFEGIDSEAAVMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILG 227

Query: 212 --SSLWEGLG-------------------------------------------------- 271
             SS  E +G                                                  
Sbjct: 228 SGSSRDESVGSSQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQ 287

Query: 272 ------------------------------------------------------------ 331
                                                                       
Sbjct: 288 QMESSEDTPKKSAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPL 347

Query: 332 --------LPSIGLGRVLEEEEEPSWV--NDRRGLLQSIGAN-IKADVVVAKDGSGKYKT 391
                   L S+    + EE E P WV  + RR L +    N IKA+VVVAKDGSGK KT
Sbjct: 348 RKLNPLNKLDSLKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKT 407

Query: 392 ITEALQEVPKKSNTTFIIYIKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITARENFV-DG 451
           I +AL  VP K+   F+I+IKEGVY+E+V V KKM +VM +GDGPTKT IT    F+ D 
Sbjct: 408 IAQALAMVPMKNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQ 467

Query: 452 TSTFKTATVSVIGTNFIGKDLWFENSAGAIKHQAVALRVQSDMSIFYNCRMDGYQDTLYT 511
             T++TA+V+V G  F+ KD+ FEN+AGA +HQAVALRV +D ++F+NC M+GYQDTLY 
Sbjct: 468 VGTYRTASVAVNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYV 527

Query: 512 HAHRQFYRDCTITGTIDFIFGNAAVVFQNCKIFVRKPLENQKCIVTAQGRTEKKEPTGIV 541
           H HRQFYR+C ++GTIDF+FG+A  VFQNC+  +R+P+E+Q+CIVTAQGR +++E TGIV
Sbjct: 528 HTHRQFYRNCRVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIV 587

The following BLAST results are available for this feature:

BLAST of CmoCh06G015160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GX86	5.0e-163	51.24	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q3E8Z8	2.7e-161	49.20	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	3.3e-151	48.66	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	1.7e-134	43.32	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
O23447	1.2e-127	38.72	Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmoCh06G015160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ET06	0.0e+00	100.00	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437278 PE=3 SV=1	[more]
A0A6J1IBZ7	2.3e-304	94.37	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472271 PE=3 SV=1	[more]
A0A6J1IE10	1.5e-271	83.42	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472305 PE=3 SV=1	[more]
A0A6J1I9B3	1.3e-270	82.88	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472272 PE=3 SV=1	[more]
A0A6J1G2K0	4.8e-270	83.42	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450212 PE=3 SV=1	[more]

BLAST of CmoCh06G015160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05610.1	3.5e-164	51.24	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G27870.1	1.9e-162	49.20	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	2.4e-152	48.66	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	1.2e-135	43.32	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	8.2e-129	38.72	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 27..191 e-value: 2.8E-28 score: 109.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..165 e-value: 3.4E-23 score: 82.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 13..165 e-value: 4.8E-23 score: 79.4
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 232..528 e-value: 2.7E-143 score: 476.1
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 226..542 e-value: 9.4E-120 score: 401.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..186 e-value: 3.1E-29 score: 103.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..185
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 16..543
None	No IPR available	PANTHER	PTHR31707:SF337	PECTINESTERASE	coord: 16..543
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 34..186 e-value: 1.32105E-39 score: 139.496
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 376..385
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 229..541

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G015160.1	CmoCh06G015160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity