CmoCh06G013510 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G013510
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionureidoglycolate hydrolase
LocationCmo_Chr06: 10005728 .. 10008524 (+)
RNA-Seq ExpressionCmoCh06G013510
SyntenyCmoCh06G013510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAATGTCACAGTCACGCTCACACGAGTAATTGTAAAGGCGAGCGTCGAATTTGCAGCCTCCAGCGGACGAGTCACGGGCCGGTTTATCCCCGATTCCATCACCACTTCCCTTCCCTTGGAGTCCAATCATTGTCGTCCCCCGCTATCATCACCCACCATGCTTCCTCTTCACCAATGCATCGCCCCCCTCCTACTTCACTTCATCTTCCTCGCACCTTTTTCGCTGGCCATAGCTCAGAATCATGAAGACCCAACCATCAGAACCATGGAGGCTTTCTCCGGCTATCCTCTTCACGAACCCTCCTCCTCTTCTATCCCAAACCCCATTTCTTCTCTCCTCGTCGATGCGGATTCCTTGGTGAAACAGGTATGGACGTAAATGGATCCTAATCGTACTGGGTTTTGATCGGATGTATTAGTTAATCAATTGTTTTGCGGATTTGGGCATTTAGATTGAGGAGCTTTCTACTTTCTCGGATTCTCCTTCCCCATCGGTTACCAGGGTTCTTTACTCTGAAAAGGATGTATTGGCGCGAAGGCATGGACTCCGGCTATTCATCTAATTTCTTTGTATTTCTTCTGTGTTCATTCCAAAATGGGACTGATTATGTGCTTGCAGGTATGTCAAGAACTTGATGGGGCTCTCTGGTCTCTTTGTTAGAGAGGATGCTGTCGGTAACATATTCGGTAGATGGTGAGTGAGTCTGCAATGGTCACCCATGTCATTAGTATTGTATTATGCATAACATCTCTAGTCTTGTTTACTTTATGTAGGGAGGGCTCTGAACCAGAGCTTCCTGCTGTTGCAACAGGCTCTCATGTTGATGCAATTCCATTCTCTGGCAAATACGATGGAGTTGTTGGTGTTTTAGGTGCTTTAGAAGCCATCAATGTCTTGAAAAGGTTTGATATCCTACTTTTACCAACAGTTTTAAAGGGTCTCTCAAACTAAATGGAAGGAACCAGCGGGTAGCTGATATTGTCCACTTTAGGCTTTTCCTTTCGAGCTTTTCCTCCGTTTCCACAGTCTTATAAAAGATGCTTCCTTCTTCTCACCAAACGCTTCGCTCTCTTCCCCACCAAACCAGATAAAATCTTTGTAGTTTTCAAGGGGCTGACTTAGAATTTGGCAGGTCGGGGTTCAAACCAAAGAGGTCTCTAGAAATTATCTTCTTTACCTCTGAGGAACCTACACGCTTTGGAATCAGCTGCTTGGGTAGCCGCTTGATGGCAGGGAGTGATGCACTTGCTAAAGCTTTGGAGACAACTGTTGATGCTCAGAATATATCTTTTATTGAAGCTGCTAGCTCTGCTGGGTACTTGACTGATTCAGCTCAGATATCAACGGTGTTTCTGAAGAAAGGAAGTTACTCTGCTTTCCTGGAATTGCACATAGAGCAGGGGCCCATTCTGGAGGAAGAAGGTATATCTATAGGAGTAGTGACTGCCATTGCAGCTCCTGCTAGTATAAAGGTGGAGTTTGAAGGCACTGGAGGCCATGCCGGTGCTGTACTGATGCCATATAGGTGAAATTATATAGCCATCAACATTTCATTGTTTTTTTTTTTTCATCAGACAATTGGAATCCAAACTTGTTCTTATTTGATCAGAAATGACGCTGGACTGGCAGCAGCTGAGCTAGCACTAGCAGTTGAGAAGCATGTATTGAGCTCTGGATCGATCGATACTGTCGGTACCGTTGGTAATTCTTCATAACCAAGTGTTTATTATGTCTTGTCCTAGCATCATCCTATATCTGCCCACCTTTCCGAACATGATCTTGATTAAATGAAACTTTTCAAGGTATTCTGGAGCTTCATCCTGGAGCAATCAACAGCATTCCAAGCAAATCACATCTGGAAATAGGTTGAGATTTTGTTGTAACTCTTGATTATCTTGTTTAGCTGCATGATGGTCGTGTACTTCATCTTCATTGTTTTTTCTTGTCTGGTAACAGACACCAGAGACATTGATGAAGAACGCAGAAATACTGTCATTAAGAAGATTCATGAATCTGCAACTGAAATAGCTCAAAGGCGACGGGTCAAACTATCAGAATTCAAAATTATAAACCAGGATCCACCAGCACATTCTGACAAATCAGTAATAGAGGCAATGGTTTCTTCAACAAAAGAGCTCAATCTCACACACAAGCTGATGATCAGCAGAGCATACCATGATTCACTGTTTATGGCCAGGTTACAGAGTTTTATCATCAATTCTCTAAATACATAGCTATTAGTTTCTCCGTATTCTTTCAGTGAACAATGCTGAATTTGCATTTTCACTACCTTGCAGGATATCCCCAATGGGCATGATATTCATTCCTTGCTATAAAGGTATTAATCTACTCTGTATCCTCTAGTAAATAGCACAAGTTGGAAGCACCTAACCTTATAATGAGTTGAGCTTGTGCCATCAAGAAAATCTGAGTCAATTTTCATGCTACTGCTTTTGCAGGGTACAGCCACAAGCCTGAAGAATTCTCCTCTCCCCAGGATATATCAAATGGAGTCAAGGTATTAGCCCTTACCCTTGCCAAATTGTCCCTGCAGTGATATCTTATTCCAAATTCCTGTAAAGTTGATTGTTCACGTCCTTGTTTCATTGGAGCCTTGTTCCCTGTGAACTTGTTTACAATAAAGTTTGAATTTTCTCCCATTACTGAATTTTCATGGTGGGTGAGTTTAGAACTAGATGATTAGTGTGTGTGAATGCAAAAGAAAGAGACAAGGATCAGCCTGTTTATCCAATTATATTTGCAATTTGAAGTTGGTAGTTCAATTACAAAGAAA

mRNA sequence

GTGAATGTCACAGTCACGCTCACACGAGTAATTGTAAAGGCGAGCGTCGAATTTGCAGCCTCCAGCGGACGAGTCACGGGCCGGTTTATCCCCGATTCCATCACCACTTCCCTTCCCTTGGAGTCCAATCATTGTCGTCCCCCGCTATCATCACCCACCATGCTTCCTCTTCACCAATGCATCGCCCCCCTCCTACTTCACTTCATCTTCCTCGCACCTTTTTCGCTGGCCATAGCTCAGAATCATGAAGACCCAACCATCAGAACCATGGAGGCTTTCTCCGGCTATCCTCTTCACGAACCCTCCTCCTCTTCTATCCCAAACCCCATTTCTTCTCTCCTCGTCGATGCGGATTCCTTGGTGAAACAGATTGAGGAGCTTTCTACTTTCTCGGATTCTCCTTCCCCATCGGTTACCAGGGTTCTTTACTCTGAAAAGGATGTATTGGCGCGAAGGTATGTCAAGAACTTGATGGGGCTCTCTGGTCTCTTTGTTAGAGAGGATGCTGTCGGTAACATATTCGGTAGATGGGAGGGCTCTGAACCAGAGCTTCCTGCTGTTGCAACAGGCTCTCATGTTGATGCAATTCCATTCTCTGGCAAATACGATGGAGTTGTTGGTGTTTTAGGTGCTTTAGAAGCCATCAATGTCTTGAAAAGGTCGGGGTTCAAACCAAAGAGGTCTCTAGAAATTATCTTCTTTACCTCTGAGGAACCTACACGCTTTGGAATCAGCTGCTTGGGTAGCCGCTTGATGGCAGGGAGTGATGCACTTGCTAAAGCTTTGGAGACAACTGTTGATGCTCAGAATATATCTTTTATTGAAGCTGCTAGCTCTGCTGGGTACTTGACTGATTCAGCTCAGATATCAACGGTGTTTCTGAAGAAAGGAAGTTACTCTGCTTTCCTGGAATTGCACATAGAGCAGGGGCCCATTCTGGAGGAAGAAGGTATATCTATAGGAGTAGTGACTGCCATTGCAGCTCCTGCTAGTATAAAGGTGGAGTTTGAAGGCACTGGAGGCCATGCCGGTGCTGTACTGATGCCATATAGAAATGACGCTGGACTGGCAGCAGCTGAGCTAGCACTAGCAGTTGAGAAGCATGTATTGAGCTCTGGATCGATCGATACTGTCGGTACCGTTGGTATTCTGGAGCTTCATCCTGGAGCAATCAACAGCATTCCAAGCAAATCACATCTGGAAATAGACACCAGAGACATTGATGAAGAACGCAGAAATACTGTCATTAAGAAGATTCATGAATCTGCAACTGAAATAGCTCAAAGGCGACGGGTCAAACTATCAGAATTCAAAATTATAAACCAGGATCCACCAGCACATTCTGACAAATCAGTAATAGAGGCAATGGTTTCTTCAACAAAAGAGCTCAATCTCACACACAAGCTGATGATCAGCAGAGCATACCATGATTCACTGTTTATGGCCAGGATATCCCCAATGGGCATGATATTCATTCCTTGCTATAAAGGGTACAGCCACAAGCCTGAAGAATTCTCCTCTCCCCAGGATATATCAAATGGAGTCAAGGTATTAGCCCTTACCCTTGCCAAATTGTCCCTGCAGTGATATCTTATTCCAAATTCCTGTAAAGTTGATTGTTCACGTCCTTGTTTCATTGGAGCCTTGTTCCCTGTGAACTTGTTTACAATAAAGTTTGAATTTTCTCCCATTACTGAATTTTCATGGTGGGTGAGTTTAGAACTAGATGATTAGTGTGTGTGAATGCAAAAGAAAGAGACAAGGATCAGCCTGTTTATCCAATTATATTTGCAATTTGAAGTTGGTAGTTCAATTACAAAGAAA

Coding sequence (CDS)

ATGCTTCCTCTTCACCAATGCATCGCCCCCCTCCTACTTCACTTCATCTTCCTCGCACCTTTTTCGCTGGCCATAGCTCAGAATCATGAAGACCCAACCATCAGAACCATGGAGGCTTTCTCCGGCTATCCTCTTCACGAACCCTCCTCCTCTTCTATCCCAAACCCCATTTCTTCTCTCCTCGTCGATGCGGATTCCTTGGTGAAACAGATTGAGGAGCTTTCTACTTTCTCGGATTCTCCTTCCCCATCGGTTACCAGGGTTCTTTACTCTGAAAAGGATGTATTGGCGCGAAGGTATGTCAAGAACTTGATGGGGCTCTCTGGTCTCTTTGTTAGAGAGGATGCTGTCGGTAACATATTCGGTAGATGGGAGGGCTCTGAACCAGAGCTTCCTGCTGTTGCAACAGGCTCTCATGTTGATGCAATTCCATTCTCTGGCAAATACGATGGAGTTGTTGGTGTTTTAGGTGCTTTAGAAGCCATCAATGTCTTGAAAAGGTCGGGGTTCAAACCAAAGAGGTCTCTAGAAATTATCTTCTTTACCTCTGAGGAACCTACACGCTTTGGAATCAGCTGCTTGGGTAGCCGCTTGATGGCAGGGAGTGATGCACTTGCTAAAGCTTTGGAGACAACTGTTGATGCTCAGAATATATCTTTTATTGAAGCTGCTAGCTCTGCTGGGTACTTGACTGATTCAGCTCAGATATCAACGGTGTTTCTGAAGAAAGGAAGTTACTCTGCTTTCCTGGAATTGCACATAGAGCAGGGGCCCATTCTGGAGGAAGAAGGTATATCTATAGGAGTAGTGACTGCCATTGCAGCTCCTGCTAGTATAAAGGTGGAGTTTGAAGGCACTGGAGGCCATGCCGGTGCTGTACTGATGCCATATAGAAATGACGCTGGACTGGCAGCAGCTGAGCTAGCACTAGCAGTTGAGAAGCATGTATTGAGCTCTGGATCGATCGATACTGTCGGTACCGTTGGTATTCTGGAGCTTCATCCTGGAGCAATCAACAGCATTCCAAGCAAATCACATCTGGAAATAGACACCAGAGACATTGATGAAGAACGCAGAAATACTGTCATTAAGAAGATTCATGAATCTGCAACTGAAATAGCTCAAAGGCGACGGGTCAAACTATCAGAATTCAAAATTATAAACCAGGATCCACCAGCACATTCTGACAAATCAGTAATAGAGGCAATGGTTTCTTCAACAAAAGAGCTCAATCTCACACACAAGCTGATGATCAGCAGAGCATACCATGATTCACTGTTTATGGCCAGGATATCCCCAATGGGCATGATATTCATTCCTTGCTATAAAGGGTACAGCCACAAGCCTGAAGAATTCTCCTCTCCCCAGGATATATCAAATGGAGTCAAGGTATTAGCCCTTACCCTTGCCAAATTGTCCCTGCAGTGA

Protein sequence

MLPLHQCIAPLLLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSLLVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNIFGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIFFTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVFLKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRNDAGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRNTVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISRAYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ
Homology
BLAST of CmoCh06G013510 vs. ExPASy Swiss-Prot
Match: Q8VXY9 (Ureidoglycolate hydrolase OS=Arabidopsis thaliana OX=3702 GN=UAH PE=1 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 3.6e-202
Identity = 372/465 (80.00%), Postives = 407/465 (87.53%), Query Frame = 0

Query: 12  LLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSLLVDADSLVKQI 71
           LL    L P SLA  Q HE  +IRTME FSGYP+HEP      N  SSL VDA  L  QI
Sbjct: 13  LLLISLLLPSSLAQQQQHE--SIRTMEDFSGYPIHEPGQFGSINLASSLSVDAPGLQNQI 72

Query: 72  EELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNIFGRWEGSEPEL 131
           +ELS+FSD+PSPSVTRVLY++KDV ARRYVKNLM L+GL VREDAVGNIFG+W+G EP L
Sbjct: 73  DELSSFSDAPSPSVTRVLYTDKDVSARRYVKNLMALAGLTVREDAVGNIFGKWDGLEPNL 132

Query: 132 PAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIFFTSEEPTRFGI 191
           PAVATGSH+DAIP+SGKYDGVVGVLGA+EAINVLKRSGFKPKRSLEII FTSEEPTRFGI
Sbjct: 133 PAVATGSHIDAIPYSGKYDGVVGVLGAIEAINVLKRSGFKPKRSLEIILFTSEEPTRFGI 192

Query: 192 SCLGSRLMAGSDALAKALETT-VDAQNISFIEAASSAGYLTD-SAQISTVFLKKGSYSAF 251
           SCLGSRL+AGS  LA+AL+TT VD QN+SFIEAA SAGY  D    +S+VFLKKGSY AF
Sbjct: 193 SCLGSRLLAGSKELAEALKTTVVDGQNVSFIEAARSAGYAEDKDDDLSSVFLKKGSYFAF 252

Query: 252 LELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRNDAGLAAAELA 311
           LELHIEQGPILE+EG+ IGVVTAIAAPAS+KVEFEG GGHAGAVLMPYRNDAGLAAAELA
Sbjct: 253 LELHIEQGPILEDEGLDIGVVTAIAAPASLKVEFEGNGGHAGAVLMPYRNDAGLAAAELA 312

Query: 312 LAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRNTVIKKIHES 371
           LAVEKHVL S SIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDE RRNTVIKKI ES
Sbjct: 313 LAVEKHVLESESIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEARRNTVIKKIQES 372

Query: 372 ATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISRAYHDSLFMA 431
           A  IA++R+VKLSEFKI+NQDPPA SDK VI+ M  +  ELNL+HK+MISRAYHDSLFMA
Sbjct: 373 ANTIAKKRKVKLSEFKIVNQDPPALSDKLVIKKMAEAATELNLSHKMMISRAYHDSLFMA 432

Query: 432 RISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSL 475
           RISPMGMIFIPCYKGYSHKPEE+SSP+D++NGVKVL+LTLAKLSL
Sbjct: 433 RISPMGMIFIPCYKGYSHKPEEYSSPEDMANGVKVLSLTLAKLSL 475

BLAST of CmoCh06G013510 vs. ExPASy Swiss-Prot
Match: Q2QMN7 (Ureidoglycolate hydrolase OS=Oryza sativa subsp. japonica OX=39947 GN=UAH PE=1 SV=2)

HSP 1 Score: 649.0 bits (1673), Expect = 4.0e-185
Identity = 336/443 (75.85%), Postives = 379/443 (85.55%), Query Frame = 0

Query: 35  RTMEAFSGYPL--HEPSSSSIPNPISSLLVDADSLVKQIEELSTFSDSPSPSVTRVLYSE 94
           RTME F+G+P   +           S   VD+D L +QI+EL++FSDSP PSVTRVLYS+
Sbjct: 42  RTMEEFAGFPASDYRGDGGGGSGGSSPFYVDSDGLQRQIDELASFSDSPVPSVTRVLYSD 101

Query: 95  KDVLARRYVKNLMGLSGLFVREDAVGNIFGRWEGSEPELPAVATGSHVDAIPFSGKYDGV 154
           KDV ARRY+K +M   GL +REDAVGNIFGRWEGSE  L AVATGSHVDAIPFSGKYDGV
Sbjct: 102 KDVQARRYIKGIMNQLGLSIREDAVGNIFGRWEGSEAGLGAVATGSHVDAIPFSGKYDGV 161

Query: 155 VGVLGALEAINVLKRSGFKPKRSLEIIFFTSEEPTRFGISCLGSRLMAGSDALAKALETT 214
           VGVLGALEAI +LKRSGF+PKRSLE+I FTSEEPTRFGISCLGSRLMAGS+ LA++L+ T
Sbjct: 162 VGVLGALEAIRMLKRSGFQPKRSLEVIMFTSEEPTRFGISCLGSRLMAGSEELARSLKET 221

Query: 215 VDAQNISFIEAASSAGYLTDSAQISTVFLKKGSYSAFLELHIEQGPILEEEGISIGVVTA 274
           VD QN+SF +AA SAGY     ++  VFLKK  Y AF+ELHIEQGPILE+EGI IGVVTA
Sbjct: 222 VDNQNVSFFDAADSAGYKMHPEELHNVFLKKDDYFAFVELHIEQGPILEKEGIKIGVVTA 281

Query: 275 IAAPASIKVEFEGTGGHAGAVLMPYRNDAGLAAAELALAVEKHVLSSGSIDTVGTVGILE 334
           IAAPASIKVEFEG GGHAGAVLMP RNDAGLAAAELALAVEKHVL SGSIDTVGTVGIL+
Sbjct: 282 IAAPASIKVEFEGNGGHAGAVLMPARNDAGLAAAELALAVEKHVLESGSIDTVGTVGILQ 341

Query: 335 LHPGAINSIPSKSHLEIDTRDIDEERRNTVIKKIHESATEIAQRRRVKLSEFKIINQDPP 394
           LHPGAINSIPSKSH+EID RDIDE+RRN VI+K+H+SA EI++ R V LSEFKIINQDPP
Sbjct: 342 LHPGAINSIPSKSHVEIDVRDIDEKRRNNVIEKVHQSAIEISKNRGVLLSEFKIINQDPP 401

Query: 395 AHSDKSVIEAMVSSTKELNLTHKLMISRAYHDSLFMARISPMGMIFIPCYKGYSHKPEEF 454
           A SDKSVI AM  + K+LNL +KLMISRAYHDSLFMARISPMGMIFIPCYKGYSHKPEE+
Sbjct: 402 ALSDKSVISAMEFAAKQLNLEYKLMISRAYHDSLFMARISPMGMIFIPCYKGYSHKPEEY 461

Query: 455 SSPQDISNGVKVLALTLAKLSLQ 476
           +SP+D++NGVKVLAL +A+LSLQ
Sbjct: 462 ASPEDMANGVKVLALAMARLSLQ 484

BLAST of CmoCh06G013510 vs. ExPASy Swiss-Prot
Match: Q53389 (N-carbamoyl-L-amino acid hydrolase OS=Geobacillus stearothermophilus OX=1422 GN=amaB PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 3.5e-64
Identity = 152/413 (36.80%), Postives = 231/413 (55.93%), Query Frame = 0

Query: 61  LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI 120
           ++  + L +++ EL      PS  VTR+ ++ ++  A+  V + M  +GLFV EDA GN+
Sbjct: 1   MIQGERLWQRLMELGEVGKQPSGGVTRLSFTAEERRAKDLVASYMREAGLFVYEDAAGNL 60

Query: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180
            GR EG+ P+   V  GSH+D++   G +DG +GVL  +E +  +   G      +E++ 
Sbjct: 61  IGRKEGTNPDATVVLVGSHLDSVYNGGCFDGPLGVLAGVEVVQTMNEHGVVTHHPIEVVA 120

Query: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240
           FT EE  RF    +GSR MAG+    +ALE   DA+ IS  EA   AG   D  ++    
Sbjct: 121 FTDEEGARFRFGMIGSRAMAGT-LPPEALECR-DAEGISLAEAMKQAG--LDPDRLPQAA 180

Query: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300
            K G+  A++ELHIEQG +LEE G+ +G+VT IA    +K   EG   HAGA  M  R D
Sbjct: 181 RKPGTVKAYVELHIEQGRVLEETGLPVGIVTGIAGLIWVKFTIEGKAEHAGATPMSLRRD 240

Query: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360
              AAA++ + +E+    +G+  TVGTVG L ++PG IN IP +    +D RD+  E R+
Sbjct: 241 PMAAAAQIIIVIEEEARRTGT--TVGTVGQLHVYPGGINVIPERVEFVLDLRDLKAEVRD 300

Query: 361 TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR 420
            V K I   A  IA+ R V+++  + + + PP      V  A  ++ ++L      + S 
Sbjct: 301 QVWKAIAVRAETIAKERNVRVTTER-LQEMPPVLCSDEVKRAAEAACQKLGYPSFWLPSG 360

Query: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLS 474
           A HDS+ +A I P+GMIF+    G SH P E+S+ +D + G +VL  T+ +L+
Sbjct: 361 AAHDSVQLAPICPIGMIFVRSQDGVSHSPAEWSTKEDCAAGAEVLYHTVWQLA 406

BLAST of CmoCh06G013510 vs. ExPASy Swiss-Prot
Match: P37113 (N-carbamoyl-L-amino acid hydrolase OS=Geobacillus stearothermophilus OX=1422 GN=amaB PE=1 SV=2)

HSP 1 Score: 242.7 bits (618), Expect = 8.7e-63
Identity = 151/413 (36.56%), Postives = 229/413 (55.45%), Query Frame = 0

Query: 61  LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI 120
           ++  + L +++ EL      PS  VTR+ ++ ++  A+  V + M  +GLFV EDA GN+
Sbjct: 1   MIQGERLWQRLMELGEVGKQPSGGVTRLSFTAEERRAKDLVASYMREAGLFVYEDAAGNL 60

Query: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180
            GR EG+ P+   V  GSH+D++   G +DG +GVL  +E +  +   G      +E++ 
Sbjct: 61  IGRKEGTNPDATVVLVGSHLDSVYNGGCFDGPLGVLAGVEVVQTMNEHGVVTHHPIEVVA 120

Query: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240
           FT EE  RF    +GSR MAG+    +ALE   DA+ IS  EA   AG   D  ++    
Sbjct: 121 FTDEEGARFRFGMIGSRAMAGT-LPPEALECR-DAEGISLAEAMKQAG--LDPDRLPQAA 180

Query: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300
            K G+  A++ELHIEQG +LEE G+ +G+VT IA    +K    G   HAGA  M  R D
Sbjct: 181 RKPGTVKAYVELHIEQGRVLEEAGLPVGIVTGIAGLIWVKFTIAGPAEHAGATPMSLRRD 240

Query: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360
              AAA++ + +E+    +G+  TVGTVG L ++PG IN IP +    +D RD+  E R+
Sbjct: 241 PMAAAAQIIIVIEEEARRTGT--TVGTVGQLHVYPGGINVIPERVEFVLDLRDLKAEVRD 300

Query: 361 TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR 420
            V K I   A  IA+ R V+L+  + + +  P    + V +A   + K+L      + S 
Sbjct: 301 QVWKAIAVRAETIAKERNVRLTTER-LQEMAPVLCSEVVKQAAERACKQLGYPPFWLPSG 360

Query: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLS 474
           A HD + +A I P+GMIF+    G SH P E+S+ +D + G +VL  T+ +L+
Sbjct: 361 AAHDGVQLAPICPIGMIFVRSQDGVSHSPAEWSTKEDCAVGAEVLYHTVWQLA 406

BLAST of CmoCh06G013510 vs. ExPASy Swiss-Prot
Match: Q57051 (Uncharacterized hydrolase HI_0588 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0588 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.5e-62
Identity = 146/395 (36.96%), Postives = 227/395 (57.47%), Query Frame = 0

Query: 71  IEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNIFGRWEGSEPE 130
           IE+L+  S  P+  +TR+ ++E+D  A   +  L     L +R D++GN+F R  G E  
Sbjct: 12  IEKLAFISSVPN-ELTRLAFTEEDEKAHNMIIELCKEYDLSIRRDSIGNLFIRKAGKEDF 71

Query: 131 LPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIFFTSEEPTRFG 190
           LPAVA GSH+D +  +GK+DG +G +  LE +  L     + +  LE+I FT EE +RF 
Sbjct: 72  LPAVAFGSHIDTVVNAGKFDGPLGSVAGLEILLQLCEQNIQTRYPLELIIFTCEESSRFN 131

Query: 191 ISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVFLKKGSYSAFL 250
            + LGS++M G     K L +  D Q     EA +  G   +    +    K+  +  F 
Sbjct: 132 FATLGSKVMCGIVNQEK-LSSLRDKQGKGLSEAMAEVGMNFNLVNQAKRDAKE--FKCFF 191

Query: 251 ELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRNDAGLAAAELAL 310
           ELHIEQGP LE EG +IGVVT IAAP    V+ +G   H+GA  M YR+DA L  +EL+L
Sbjct: 192 ELHIEQGPRLENEGKTIGVVTGIAAPIRAIVKIKGQADHSGATAMHYRHDALLGGSELSL 251

Query: 311 AVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRNTVIKKIHESA 370
           A+E+  + +G   TV TVG +   PG +N +P    L +D R    + R++V + + E  
Sbjct: 252 AIERAAIQAGH-STVATVGNITAKPGVMNVVPGYCELLVDIRGTHVQARDSVFELLQEEI 311

Query: 371 TEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISRAYHDSLFMAR 430
           ++++++R + L E ++I++D P    ++++  +  +   L  ++++M S A HD++ MA 
Sbjct: 312 SKVSEKRGL-LIELQLISKDNPIILPENMVNQIAETAHSLGYSYEIMPSGAGHDAMHMAT 371

Query: 431 ISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVL 466
           + P GMIFIP + G SH P EF+  +DI  G+KVL
Sbjct: 372 LCPTGMIFIPSHLGISHNPLEFTDWKDIEAGIKVL 400

BLAST of CmoCh06G013510 vs. ExPASy TrEMBL
Match: A0A6J1G3T9 (ureidoglycolate hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450481 PE=4 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.2e-261
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MLPLHQCIAPLLLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSL 60
           MLPLHQCIAPLLLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSL
Sbjct: 1   MLPLHQCIAPLLLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSL 60

Query: 61  LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI 120
           LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI
Sbjct: 61  LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI 120

Query: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180
           FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF
Sbjct: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180

Query: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240
           FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF
Sbjct: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240

Query: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300
           LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND
Sbjct: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300

Query: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360
           AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN
Sbjct: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360

Query: 361 TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR 420
           TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR
Sbjct: 361 TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR 420

Query: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ 476
           AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ
Sbjct: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ 475

BLAST of CmoCh06G013510 vs. ExPASy TrEMBL
Match: A0A6J1IDQ9 (ureidoglycolate hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111474417 PE=4 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.3e-259
Identity = 469/475 (98.74%), Postives = 473/475 (99.58%), Query Frame = 0

Query: 1   MLPLHQCIAPLLLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSL 60
           MLPLHQCIAPLLLHFIFLAPFSLA+AQNHEDPTIRTMEAFSGY LHEPSSSSIPNPISSL
Sbjct: 1   MLPLHQCIAPLLLHFIFLAPFSLALAQNHEDPTIRTMEAFSGYTLHEPSSSSIPNPISSL 60

Query: 61  LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI 120
           LVDADSLVKQIEELSTFSDSPSPSVTR+LYSE DVLARRYVKNLMGLSGLFVREDAVGNI
Sbjct: 61  LVDADSLVKQIEELSTFSDSPSPSVTRILYSENDVLARRYVKNLMGLSGLFVREDAVGNI 120

Query: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180
           FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF
Sbjct: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180

Query: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240
           FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF
Sbjct: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240

Query: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300
           LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND
Sbjct: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300

Query: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360
           AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN
Sbjct: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360

Query: 361 TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR 420
           +VIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKS+IEAMVSSTKELNLTHKLMISR
Sbjct: 361 SVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSIIEAMVSSTKELNLTHKLMISR 420

Query: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ 476
           AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ
Sbjct: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ 475

BLAST of CmoCh06G013510 vs. ExPASy TrEMBL
Match: A0A0A0LA05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142980 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.3e-236
Identity = 427/475 (89.89%), Postives = 453/475 (95.37%), Query Frame = 0

Query: 1   MLPLHQCIAPLLLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSL 60
           MLPL Q +A LLLHFIF++ FS A+A NH+DPTIRTMEAFSGYP+HEPSS     PI+SL
Sbjct: 1   MLPLLQLVA-LLLHFIFISSFSAALAHNHDDPTIRTMEAFSGYPVHEPSSHGFSTPITSL 60

Query: 61  LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI 120
            VDAD+LVKQIEELSTFSDSP+PSVTR+LY+EKDVLARR+VK+LMGLSGL VREDAVGNI
Sbjct: 61  SVDADTLVKQIEELSTFSDSPAPSVTRILYTEKDVLARRFVKSLMGLSGLSVREDAVGNI 120

Query: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180
           FGRW+GSEP+LPAVATGSH DAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF
Sbjct: 121 FGRWDGSEPDLPAVATGSHADAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180

Query: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240
           FTSEEPTRFGISCLGSRL+AGSDALAKALETTVD QNISFI AASSAGYLTD AQISTVF
Sbjct: 181 FTSEEPTRFGISCLGSRLLAGSDALAKALETTVDGQNISFISAASSAGYLTDPAQISTVF 240

Query: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300
           LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPAS+KVEFEGTGGHAGAVLMPYRND
Sbjct: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASLKVEFEGTGGHAGAVLMPYRND 300

Query: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360
           AGLAAAELALAVEKHVL SGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN
Sbjct: 301 AGLAAAELALAVEKHVLDSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360

Query: 361 TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR 420
            V+KKIHESA EIA+RRRVKLSEFKIINQDPPAHSDKS+IEAMVS+ K+LNLTHKLMISR
Sbjct: 361 IVLKKIHESANEIAKRRRVKLSEFKIINQDPPAHSDKSIIEAMVSAAKDLNLTHKLMISR 420

Query: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ 476
           AYHDSLFMAR+SPMGMIFIPCYKGYSHKPEEF+S +D+SNGVKVLALTLAKLSLQ
Sbjct: 421 AYHDSLFMARVSPMGMIFIPCYKGYSHKPEEFASLKDMSNGVKVLALTLAKLSLQ 474

BLAST of CmoCh06G013510 vs. ExPASy TrEMBL
Match: A0A5D3D0C3 (Ureidoglycolate hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001150 PE=4 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.5e-235
Identity = 424/475 (89.26%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MLPLHQCIAPLLLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSL 60
           MLPL Q IA LLL FIF++ FS A+A NH+DP IRTMEAFSGYP+HEPS     NPI+SL
Sbjct: 1   MLPLLQLIA-LLLPFIFISSFSAALAHNHDDPAIRTMEAFSGYPVHEPSPHGFSNPITSL 60

Query: 61  LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI 120
            VDAD+LVKQIEELSTFSDSP+PSVTR+LY+EKDVLARR+VKNLMGLSGL VREDAVGNI
Sbjct: 61  SVDADTLVKQIEELSTFSDSPAPSVTRILYTEKDVLARRFVKNLMGLSGLSVREDAVGNI 120

Query: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180
           FGRW+GSEP+LPAVATGSH DAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF
Sbjct: 121 FGRWDGSEPDLPAVATGSHADAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180

Query: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240
           FTSEEPTRFGISCLGSRL+AGSDALAKALETTVD QNISFI AASSAGYLTD A+ISTVF
Sbjct: 181 FTSEEPTRFGISCLGSRLLAGSDALAKALETTVDGQNISFISAASSAGYLTDPARISTVF 240

Query: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300
           LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPAS+KVEFEGTGGHAGAVLMPYRND
Sbjct: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASLKVEFEGTGGHAGAVLMPYRND 300

Query: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360
           AGLAAAELALAVEKHVL SGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN
Sbjct: 301 AGLAAAELALAVEKHVLDSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360

Query: 361 TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR 420
            VIKKIHESA EI++RR+VKLSEFKIINQDPPAHSDKS++EAMVS+ K+LNL+HKLMISR
Sbjct: 361 IVIKKIHESANEISKRRQVKLSEFKIINQDPPAHSDKSIVEAMVSAAKDLNLSHKLMISR 420

Query: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ 476
           AYHDSLFMAR+SPMGMIFIPCYKGYSHKPEEF+SP D+SNGVKVLALTLAKLSLQ
Sbjct: 421 AYHDSLFMARVSPMGMIFIPCYKGYSHKPEEFASPMDMSNGVKVLALTLAKLSLQ 474

BLAST of CmoCh06G013510 vs. ExPASy TrEMBL
Match: A0A1S3AWT8 (ureidoglycolate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483639 PE=4 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.5e-235
Identity = 424/475 (89.26%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MLPLHQCIAPLLLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSL 60
           MLPL Q IA LLL FIF++ FS A+A NH+DP IRTMEAFSGYP+HEPS     NPI+SL
Sbjct: 1   MLPLLQLIA-LLLPFIFISSFSAALAHNHDDPAIRTMEAFSGYPVHEPSPHGFSNPITSL 60

Query: 61  LVDADSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNI 120
            VDAD+LVKQIEELSTFSDSP+PSVTR+LY+EKDVLARR+VKNLMGLSGL VREDAVGNI
Sbjct: 61  SVDADTLVKQIEELSTFSDSPAPSVTRILYTEKDVLARRFVKNLMGLSGLSVREDAVGNI 120

Query: 121 FGRWEGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180
           FGRW+GSEP+LPAVATGSH DAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF
Sbjct: 121 FGRWDGSEPDLPAVATGSHADAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIF 180

Query: 181 FTSEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVF 240
           FTSEEPTRFGISCLGSRL+AGSDALAKALETTVD QNISFI AASSAGYLTD A+ISTVF
Sbjct: 181 FTSEEPTRFGISCLGSRLLAGSDALAKALETTVDGQNISFISAASSAGYLTDPARISTVF 240

Query: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRND 300
           LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPAS+KVEFEGTGGHAGAVLMPYRND
Sbjct: 241 LKKGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASLKVEFEGTGGHAGAVLMPYRND 300

Query: 301 AGLAAAELALAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360
           AGLAAAELALAVEKHVL SGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN
Sbjct: 301 AGLAAAELALAVEKHVLDSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRN 360

Query: 361 TVIKKIHESATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISR 420
            VIKKIHESA EI++RR+VKLSEFKIINQDPPAHSDKS++EAMVS+ K+LNL+HKLMISR
Sbjct: 361 IVIKKIHESANEISKRRQVKLSEFKIINQDPPAHSDKSIVEAMVSAAKDLNLSHKLMISR 420

Query: 421 AYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSLQ 476
           AYHDSLFMAR+SPMGMIFIPCYKGYSHKPEEF+SP D+SNGVKVLALTLAKLSLQ
Sbjct: 421 AYHDSLFMARVSPMGMIFIPCYKGYSHKPEEFASPMDMSNGVKVLALTLAKLSLQ 474

BLAST of CmoCh06G013510 vs. TAIR 10
Match: AT5G43600.1 (ureidoglycolate amidohydrolase )

HSP 1 Score: 705.7 bits (1820), Expect = 2.6e-203
Identity = 372/465 (80.00%), Postives = 407/465 (87.53%), Query Frame = 0

Query: 12  LLHFIFLAPFSLAIAQNHEDPTIRTMEAFSGYPLHEPSSSSIPNPISSLLVDADSLVKQI 71
           LL    L P SLA  Q HE  +IRTME FSGYP+HEP      N  SSL VDA  L  QI
Sbjct: 13  LLLISLLLPSSLAQQQQHE--SIRTMEDFSGYPIHEPGQFGSINLASSLSVDAPGLQNQI 72

Query: 72  EELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNIFGRWEGSEPEL 131
           +ELS+FSD+PSPSVTRVLY++KDV ARRYVKNLM L+GL VREDAVGNIFG+W+G EP L
Sbjct: 73  DELSSFSDAPSPSVTRVLYTDKDVSARRYVKNLMALAGLTVREDAVGNIFGKWDGLEPNL 132

Query: 132 PAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSGFKPKRSLEIIFFTSEEPTRFGI 191
           PAVATGSH+DAIP+SGKYDGVVGVLGA+EAINVLKRSGFKPKRSLEII FTSEEPTRFGI
Sbjct: 133 PAVATGSHIDAIPYSGKYDGVVGVLGAIEAINVLKRSGFKPKRSLEIILFTSEEPTRFGI 192

Query: 192 SCLGSRLMAGSDALAKALETT-VDAQNISFIEAASSAGYLTD-SAQISTVFLKKGSYSAF 251
           SCLGSRL+AGS  LA+AL+TT VD QN+SFIEAA SAGY  D    +S+VFLKKGSY AF
Sbjct: 193 SCLGSRLLAGSKELAEALKTTVVDGQNVSFIEAARSAGYAEDKDDDLSSVFLKKGSYFAF 252

Query: 252 LELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRNDAGLAAAELA 311
           LELHIEQGPILE+EG+ IGVVTAIAAPAS+KVEFEG GGHAGAVLMPYRNDAGLAAAELA
Sbjct: 253 LELHIEQGPILEDEGLDIGVVTAIAAPASLKVEFEGNGGHAGAVLMPYRNDAGLAAAELA 312

Query: 312 LAVEKHVLSSGSIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEERRNTVIKKIHES 371
           LAVEKHVL S SIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDE RRNTVIKKI ES
Sbjct: 313 LAVEKHVLESESIDTVGTVGILELHPGAINSIPSKSHLEIDTRDIDEARRNTVIKKIQES 372

Query: 372 ATEIAQRRRVKLSEFKIINQDPPAHSDKSVIEAMVSSTKELNLTHKLMISRAYHDSLFMA 431
           A  IA++R+VKLSEFKI+NQDPPA SDK VI+ M  +  ELNL+HK+MISRAYHDSLFMA
Sbjct: 373 ANTIAKKRKVKLSEFKIVNQDPPALSDKLVIKKMAEAATELNLSHKMMISRAYHDSLFMA 432

Query: 432 RISPMGMIFIPCYKGYSHKPEEFSSPQDISNGVKVLALTLAKLSL 475
           RISPMGMIFIPCYKGYSHKPEE+SSP+D++NGVKVL+LTLAKLSL
Sbjct: 433 RISPMGMIFIPCYKGYSHKPEEYSSPEDMANGVKVLSLTLAKLSL 475

BLAST of CmoCh06G013510 vs. TAIR 10
Match: AT4G20070.1 (allantoate amidohydrolase )

HSP 1 Score: 167.5 bits (423), Expect = 2.5e-41
Identity = 129/420 (30.71%), Postives = 200/420 (47.62%), Query Frame = 0

Query: 65  DSLVKQIEELSTFSDSPSPSVTRVLYSEKDVLARRYVKNLMGLSGLFVREDAVGNIFGRW 124
           D  V ++ EL   SD+ +  + R   S   + A   ++  M  +GL    D +GN+ GR 
Sbjct: 94  DEAVARLHELGQVSDA-ATHLERTFMSPASIRAIPLIRGWMEDAGLSTWVDYMGNVHGRV 153

Query: 125 EGSEPELPAVATGSHVDAIPFSGKYDGVVGVLGALEAINVLKRSG--FKPKRSLEIIFFT 184
           E       A+  GSH+D +  +GKYDG +G++ A+ A+ VLK  G   + KR +E+I F+
Sbjct: 154 EPKNGSSQALLIGSHMDTVIDAGKYDGSLGIISAISALKVLKIDGRLGELKRPVEVIAFS 213

Query: 185 SEEPTRFGISCLGSRLMAGSDALAKALETTVDAQNISFIEAASSAGYLTDSAQISTVFLK 244
            EE  RF  + LGS  +AG   +++ LE T D   IS  +A            +  +   
Sbjct: 214 DEEGVRFQSTFLGSAALAGIMPVSR-LEVT-DKSGISVQDALKENSIDITDENLMQLKYD 273

Query: 245 KGSYSAFLELHIEQGPILEEEGISIGVVTAIAAPASIKVEFEGTGGHAGAVLMPYRNDAG 304
             S   ++E+HIEQGP+LE  G  +GVV  IA    +KV  +G+ GHAG V M  R D  
Sbjct: 274 PASVWGYVEVHIEQGPVLEWVGYPLGVVKGIAGQTRLKVTVKGSQGHAGTVPMSMRQDPM 333

Query: 305 LAAAELALAVE-----------------KHVLSSGSIDTVGTVGILELHPGAINSIPSKS 364
             AAEL + +E                 +  + S +   V TVG +   P A N IP + 
Sbjct: 334 TGAAELIVLLESVCKNPKDYLSCNVQCNEDTVESLANSLVCTVGEISTWPSASNVIPGQV 393

Query: 365 HLEIDTRDIDEERRNTVIKKIHESATEIAQRRRVKLS-----EFKIINQDPPAHSD-KSV 424
              +D R ID+  R  ++  +     +I  +R +  S     +   +  DP      KS 
Sbjct: 394 TFTVDLRTIDDVGRKAILHDLSTRMYQICDKRSLLCSIERKHDADAVMSDPQLSLQLKSA 453

Query: 425 IE-AMVSSTKELNLTHKLMISRAYHDSLFMARISPMGMIFIPCYKGYSHKPEEFSSPQDI 459
            + A+   T E+     +++S A HD++ MA ++ +GM+F+ C  G SH P E     D+
Sbjct: 454 AQSALKKMTGEVQDEVPVLMSGAGHDAMAMAHLTKVGMLFVRCRGGISHSPAEHVLDDDV 510

BLAST of CmoCh06G013510 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 47.4 bits (111), Expect = 3.8e-05
Identity = 29/85 (34.12%), Postives = 36/85 (42.35%), Query Frame = 0

Query: 123 RWEGSEPELPAVATGSHVDAIPFS----------------------GKYDGVVGVLGALE 182
           +W GS+P LPA    SH D +PF                       G  D     +  LE
Sbjct: 76  KWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLE 135

Query: 183 AINVLKRSGFKPKRSLEIIFFTSEE 186
           AI  L+ SGFKP RS+ + F   EE
Sbjct: 136 AIRKLQASGFKPLRSVYLSFVPDEE 160

BLAST of CmoCh06G013510 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 47.4 bits (111), Expect = 3.8e-05
Identity = 29/85 (34.12%), Postives = 36/85 (42.35%), Query Frame = 0

Query: 123 RWEGSEPELPAVATGSHVDAIPFS----------------------GKYDGVVGVLGALE 182
           +W GS+P LPA    SH D +PF                       G  D     +  LE
Sbjct: 76  KWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLE 135

Query: 183 AINVLKRSGFKPKRSLEIIFFTSEE 186
           AI  L+ SGFKP RS+ + F   EE
Sbjct: 136 AIRKLQASGFKPLRSVYLSFVPDEE 160

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VXY93.6e-20280.00Ureidoglycolate hydrolase OS=Arabidopsis thaliana OX=3702 GN=UAH PE=1 SV=1[more]
Q2QMN74.0e-18575.85Ureidoglycolate hydrolase OS=Oryza sativa subsp. japonica OX=39947 GN=UAH PE=1 S... [more]
Q533893.5e-6436.80N-carbamoyl-L-amino acid hydrolase OS=Geobacillus stearothermophilus OX=1422 GN=... [more]
P371138.7e-6336.56N-carbamoyl-L-amino acid hydrolase OS=Geobacillus stearothermophilus OX=1422 GN=... [more]
Q570511.5e-6236.96Uncharacterized hydrolase HI_0588 OS=Haemophilus influenzae (strain ATCC 51907 /... [more]
Match NameE-valueIdentityDescription
A0A6J1G3T91.2e-261100.00ureidoglycolate hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450481 PE=4 SV=... [more]
A0A6J1IDQ93.3e-25998.74ureidoglycolate hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111474417 PE=4 SV=1[more]
A0A0A0LA052.3e-23689.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142980 PE=4 SV=1[more]
A0A5D3D0C37.5e-23589.26Ureidoglycolate hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3AWT87.5e-23589.26ureidoglycolate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483639 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G43600.12.6e-20380.00ureidoglycolate amidohydrolase [more]
AT4G20070.12.5e-4130.71allantoate amidohydrolase [more]
AT4G38220.13.8e-0534.12Peptidase M20/M25/M40 family protein [more]
AT4G38220.23.8e-0534.12Peptidase M20/M25/M40 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 136..470
e-value: 3.2E-31
score: 108.7
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 74..465
e-value: 1.0E-143
score: 480.7
NoneNo IPR availableGENE3D3.30.70.360coord: 275..391
e-value: 1.0E-143
score: 480.7
NoneNo IPR availablePANTHERPTHR32494:SF5ALLANTOATE DEIMINASE-RELATEDcoord: 59..470
NoneNo IPR availableCDDcd03884M20_bAScoord: 67..470
e-value: 6.00391E-171
score: 484.719
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 61..472
IPR010158Amidase, carbamoylase-typeTIGRFAMTIGR01879TIGR01879coord: 67..471
e-value: 1.6E-110
score: 367.7
IPR010158Amidase, carbamoylase-typePANTHERPTHR32494ALLANTOATE DEIMINASE-RELATEDcoord: 59..470
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 278..386

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G013510.1CmoCh06G013510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000256 allantoin catabolic process
biological_process GO:0006145 purine nucleobase catabolic process
biological_process GO:0010136 ureide catabolic process
cellular_component GO:0005783 endoplasmic reticulum
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0004848 ureidoglycolate hydrolase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016813 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines