CmoCh06G012460 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh06G012460
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	alkaline ceramidase-like
Location	Cmo_Chr06: 9419475 .. 9422279 (+)
RNA-Seq Expression	CmoCh06G012460
Synteny	CmoCh06G012460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCACGCGCTCATCGTCTTCCCTCTGGGTAAGCCCTAATTGGTTCGAACTCTCAGCTCAGTTCTCCTCTGATCGCTTCGCACCATTGACAGAAAGACCTCAGGAATTTGAATTTCAAGCTCCCAACAATGGCTCTTCTCTAGCTTAGATCCATCTCGGAAGGGTTAGCAGTTTCTTCAGCCTCTAGCATTTTTGTTTTCGTTCGGTTTTGAATTTGCCTTTTTAGGTTATTTAGTTGGATTTCTCCGCGATTTTTATGTCTAGAGATTATAAAATTTTGTTTGTTTCACAAATTTCTCGAGAAATTAGTGATGAAAAAATGGACGAAATGGAAAAAGTTCAACGTAGTTTTAAATTTGCTTCAGAAAACGTTGCCATTTGTGTCGTTGTGTTCAAGTTAGTTTACTTTTGACGCTCGTATTCGCGGCGTGAAGAATTTAGGAAGCGATTCATGAAAGCTGCAATTCGTTTCTTAGCTTGGTGCGAGCTAGGTTTGGCGGTTTCTGCTATTGGAAGTAAAATTCGAAAGATGGAGTTGATTTTTTGACATTGTCGTTTACTTGACCAAGGAATGAAGGAAGGTGGAAAGCCTCTTTTTCTGTAGGTCTTTCATATCTGCATGTTGTTTTCAGTTGGTTTGCTAGTCTATTCTCGTCATCATCCACGCCATTTTCTGTCATGAATTTGATCAATCAAGCTTGTAGTTTTCTGCAATTCTTTGATAATTCAGCCAATTTTTCGCATTAAATACATATTTCATTCAGAATGTTACAGTATTTGCTTGGGATGAATATGAAACAGTTTCTACTTTGACTTGTGTGTGTGTGTGTTCATCATTTATGCACTGACTTACTACTTGTTAAATAACATGATTGCAGGTTCGTAAATTCTGAACCATCCTAGCAGCAGGCAATTCAAGTATTATCGAGCATACTTAATCGTAGAATGGCAGAAACAATATCTAGTTTCTGGGGTCCTGTTACATCTCCTGAATGGTGTGAAAAGAACTATGTCTACTCTTCTTATATTGCAGAATTCTTCAACACAGTATCAAATATTCCGTGCCTCATTTTAGCATTTGTTGGTCTCGTAAATGCTTTGAGACAAAGGTTCGAGAAGAGGTTTAGCGTTCTTCACGTATCAAATATGATCCTTGCCATAGGAAGCATGTTGTATCATGCGACCTTGCAACGAGTGTAAGTTTTCTTCCTTTATAGCAAAGAAAAAAGAATAAAGATGACATACATGAGTCGAAGTGTTTAAGTTCGATGTACAGGTTAGAGGTTGTGAGTCAGAAAATATTTAAGCGACCTATATAAGATAGATCCCATGAGTGATCGAAATACTAAATGTCGTGGCGTGGATGTATGGGAAGAAATAGCTTGGATAGAGGAAAATATGGGTAAACCATAGCTTTATCCTCGTCCACGATAATGGAATTTTCTTTTAGAACTTTTCGGGTTCGAGGGAAAGCAAGGAGGAAACTGCTAAATAGATGATAACTAGTTTTTTAAGGGAATTTTTTGTTTGTTTTCTCAATTGCATAACGACCAAAAAATGAACTATGCTGTTCGGTTTTCTTTGTGCTCATCTTAGAAGTTAGAATCGTCTCATTTTTTCATTAATAATTGGAATTTATTCTCTCATGGATCAATTTTCTAGTCCTCCTCTCATCCCTGATTTTTATACTCGTATTATGCGTTAGGAGTTGCAACGTTTTACATTTTATTCCCCCCTCTAGCCGAGGTTTGGTACATGTATTGAAGATTTCCCTATGAGATTATTTTTTCTGAACTTCAATAATCTTTAGCAAATGTATACTGTTTTTAGCATTGGGTTGCATGTTCTGTTAAATCTTCATTCATTATAAAGTATCTTATCTATTGGATTCAATGATAAACTTATTTTCAGACAACAGCAAGGTGATGAAACACCAATGGTATGGGAAATGCTCCTCTATATCTACATCCTGTATTCGCCAGATTGGCATTACCGGAGCACGATGCCTACGTTCTTATTTCTCTACGGTGCTGCCTTTGCTGTTGCTCATGCAATCATACGTTTTGGAGCGGGCTTCAAGGTACATTATGCAATACTATGTCTTATCTGCATCCCTAGAATGTACAAGTATTATATTCACACGAACGATGTACACGCAAAGCGGCTGGCGAAGTTATACTTGACAACCATCTCGCTCGGTACTGTTTGCTGGCTTTTTGATTGCTTGTACTGCAGGAATATCTCTAGCTGGTATTTCAACCCTCAGGGTCATGCATTGTGGCATTTATTTATGGGGTTCAATTCTTATTTCGCAAATACATTTTTAATGTTTTGTCGTGCTCAACAACTCGAGTGGAACCCGAGCGTAGTCCACTTTTTGGGATTGTTTCCATACGTGAAGATTCAAAAACCAAAGAGCCAGTGATCGAAGTCGAGTTAGGTCTTGGATTGGAAGGAAAACAACATTCTTTTATCTTGAAATGGATTTTTGGTTTTGGCTGGCTATAATCAGTAGGATTAGACATTCTTTTTATCCCTTCTCATATGGATGTAAATCATACTCAGAATGGCTGTCGTTGATTTAGATTGGGTTTTACTGCAGCTGTAAAATTGTGTTTTACCTTTGGTTTATTTTACTGTTTTCGAGTTTTCGAGTTTTTTACTGTTCTTATAGAAATTGACTTATATGGAACTTTCACAGATTTGCTCTATAGAATTGTCTTTAGAGCAGTTGTTGTTTTAGTGACACAGCTTTGAGAATCAATTAGTTCATTACATATCAACTCCTGGAGAAGTGCAAATTTCAT

mRNA sequence

CCACGCGCTCATCGTCTTCCCTCTGGGTAAGCCCTAATTGGTTCGAACTCTCAGCTCAGTTCTCCTCTGATCGCTTCGCACCATTGACAGAAAGACCTCAGGAATTTGAATTTCAAGCTCCCAACAATGGCTCTTCTCTAGCTTAGATCCATCTCGGAAGGGTTCGTAAATTCTGAACCATCCTAGCAGCAGGCAATTCAAGTATTATCGAGCATACTTAATCGTAGAATGGCAGAAACAATATCTAGTTTCTGGGGTCCTGTTACATCTCCTGAATGGTGTGAAAAGAACTATGTCTACTCTTCTTATATTGCAGAATTCTTCAACACAGTATCAAATATTCCGTGCCTCATTTTAGCATTTGTTGGTCTCGTAAATGCTTTGAGACAAAGGTTCGAGAAGAGGTTTAGCGTTCTTCACGTATCAAATATGATCCTTGCCATAGGAAGCATGTTGTATCATGCGACCTTGCAACGAGTACAACAGCAAGGTGATGAAACACCAATGGTATGGGAAATGCTCCTCTATATCTACATCCTGTATTCGCCAGATTGGCATTACCGGAGCACGATGCCTACGTTCTTATTTCTCTACGGTGCTGCCTTTGCTGTTGCTCATGCAATCATACGTTTTGGAGCGGGCTTCAAGGTACATTATGCAATACTATGTCTTATCTGCATCCCTAGAATGTACAAGTATTATATTCACACGAACGATGTACACGCAAAGCGGCTGGCGAAGTTATACTTGACAACCATCTCGCTCGGTACTGTTTGCTGGCTTTTTGATTGCTTGTACTGCAGGAATATCTCTAGCTGGTATTTCAACCCTCAGGGTCATGCATTGTGGCATTTATTTATGGGGTTCAATTCTTATTTCGCAAATACATTTTTAATGTTTTGTCGTGCTCAACAACTCGAGTGGAACCCGAGCGTAGTCCACTTTTTGGGATTGTTTCCATACGTGAAGATTCAAAAACCAAAGAGCCAGTGATCGAAGTCGAGTTAGGTCTTGGATTGGAAGGAAAACAACATTCTTTTATCTTGAAATGGATTTTTGGTTTTGGCTGGCTATAATCAGTAGGATTAGACATTCTTTTTATCCCTTCTCATATGGATGTAAATCATACTCAGAATGGCTGTCGTTGATTTAGATTGGGTTTTACTGCAGCTGTAAAATTGTGTTTTACCTTTGGTTTATTTTACTGTTTTCGAGTTTTCGAGTTTTTTACTGTTCTTATAGAAATTGACTTATATGGAACTTTCACAGATTTGCTCTATAGAATTGTCTTTAGAGCAGTTGTTGTTTTAGTGACACAGCTTTGAGAATCAATTAGTTCATTACATATCAACTCCTGGAGAAGTGCAAATTTCAT

Coding sequence (CDS)

ATGGCAGAAACAATATCTAGTTTCTGGGGTCCTGTTACATCTCCTGAATGGTGTGAAAAGAACTATGTCTACTCTTCTTATATTGCAGAATTCTTCAACACAGTATCAAATATTCCGTGCCTCATTTTAGCATTTGTTGGTCTCGTAAATGCTTTGAGACAAAGGTTCGAGAAGAGGTTTAGCGTTCTTCACGTATCAAATATGATCCTTGCCATAGGAAGCATGTTGTATCATGCGACCTTGCAACGAGTACAACAGCAAGGTGATGAAACACCAATGGTATGGGAAATGCTCCTCTATATCTACATCCTGTATTCGCCAGATTGGCATTACCGGAGCACGATGCCTACGTTCTTATTTCTCTACGGTGCTGCCTTTGCTGTTGCTCATGCAATCATACGTTTTGGAGCGGGCTTCAAGGTACATTATGCAATACTATGTCTTATCTGCATCCCTAGAATGTACAAGTATTATATTCACACGAACGATGTACACGCAAAGCGGCTGGCGAAGTTATACTTGACAACCATCTCGCTCGGTACTGTTTGCTGGCTTTTTGATTGCTTGTACTGCAGGAATATCTCTAGCTGGTATTTCAACCCTCAGGGTCATGCATTGTGGCATTTATTTATGGGGTTCAATTCTTATTTCGCAAATACATTTTTAATGTTTTGTCGTGCTCAACAACTCGAGTGGAACCCGAGCGTAGTCCACTTTTTGGGATTGTTTCCATACGTGAAGATTCAAAAACCAAAGAGCCAGTGA

Protein sequence

MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRFSVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLGTVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFLGLFPYVKIQKPKSQ

Homology

[Close]

BLAST of CmoCh06G012460 vs. ExPASy Swiss-Prot
Match: Q94IB9 (Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 9.5e-117
Identity = 194/255 (76.08%), Postives = 221/255 (86.67%), Query Frame = 0


Query: 1   MAETISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKR 60
           MA+ ISSFWGPVTS  E CE NY YSSYIAEF+NT+SN+P ++LA +GLVNALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFL 120
           FS+LH+SNMILAIGSMLYHATLQ VQQQ DETPMVWE+LLY+YILYSPDWHYRSTMPTFL
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISL 180
           FLYGAAFA+ HA +RFG GFKVHY ILCL+CIPRMYKYYIHT D  AKR+AK Y+ TI +
Sbjct: 121 FLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILV 180

Query: 181 GTVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHF 240
           G++CW  D ++C+ IS W  NPQGHALWH+FM FNSY ANTFLMFCRAQQ  WNP V +F
Sbjct: 181 GSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYF 240

Query: 241 LGLFPYVKIQKPKSQ 255
           LG+ PYVKI+KPK+Q
Sbjct: 241 LGVLPYVKIEKPKTQ 255

[Close]

BLAST of CmoCh06G012460 vs. ExPASy Swiss-Prot
Match: Q9D099 (Alkaline ceramidase 3 OS=Mus musculus OX=10090 GN=Acer3 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.0e-25
Identity = 73/258 (28.29%), Postives = 124/258 (48.06%), Query Frame = 0


Query: 8   FWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRFSVLHVS 67
           +WGP TS  +WCE+NYV + ++AEF+NTVSN+  +I    G +  +R R EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYVVTLFVAEFWNTVSNLIMIIPPIFGAIQGIRDRLEKRYIAAYLA 69

Query: 68  NMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILY-------SPDWHYRSTMPTFL 127
             ++ +GS  +H TL+   Q  DE PM++   +++Y ++       S ++H   T    L
Sbjct: 70  LTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKTKSSINYHLLFT----L 129

Query: 128 FLYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISL 187
           FLY       +  ++     +V Y +L    + R   Y +       + L    LT   L
Sbjct: 130 FLYSLTVTTIYLKVKEPIFHQVMYGMLVFTLVLRSI-YIVTWVYPWLRGLGYTSLTVFLL 189

Query: 188 GTVCWLFDCLYCRNISSWY--------FNPQGHALWHLFMGFNSYFANTFLMFCRAQQLE 247
           G + W  D ++C ++ ++            Q HA WH+  G  SY    F ++ R   L 
Sbjct: 190 GFLLWNIDNIFCDSLRNFRKRVPPVLGVTTQFHAWWHILTGLGSYLHILFSLYTRTLYLR 249

Query: 248 WNPSVVHFLGLFPYVKIQ 250
           + P V    G++P V  +
Sbjct: 250 YRPKVKFLFGIWPAVMFE 262

[Close]

BLAST of CmoCh06G012460 vs. ExPASy Swiss-Prot
Match: Q9NUN7 (Alkaline ceramidase 3 OS=Homo sapiens OX=9606 GN=ACER3 PE=1 SV=3)

HSP 1 Score: 108.6 bits (270), Expect = 1.0e-22
Identity = 70/255 (27.45%), Postives = 120/255 (47.06%), Query Frame = 0


Query: 8   FWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRFSVLHVS 67
           +WGP TS  +WCE+NY  + YIAEF+NTVSN+  +I    G V ++R   EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEKRYIASYLA 69

Query: 68  NMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILY-------SPDWHYRSTMPTFL 127
             ++ +GS  +H TL+   Q  DE PM++   +++Y ++       S ++H   T    L
Sbjct: 70  LTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHLLFT----L 129

Query: 128 FLYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISL 187
            L+       +  ++     +V Y +L    + R   Y +       + L    L    L
Sbjct: 130 VLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSI-YIVTWVYPWLRGLGYTSLGIFLL 189

Query: 188 GTVCWLFDCLYCRNISSWY--------FNPQGHALWHLFMGFNSYFANTFLMFCRAQQLE 247
           G + W  D ++C ++ ++            Q HA WH+  G  SY    F ++ R   L 
Sbjct: 190 GFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTLYLR 249

[Close]

BLAST of CmoCh06G012460 vs. ExPASy Swiss-Prot
Match: Q02896 (Alkaline ceramidase YDC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDC1 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.6e-18
Identity = 79/278 (28.42%), Postives = 128/278 (46.04%), Query Frame = 0


Query: 5   ISSFWG-PVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRFSVL 64
           I  +WG P +  +WCE+NYV S YIAE+ NT++N   L+ AF    +A R + E R+ ++
Sbjct: 12  IEGYWGKPTSLIDWCEENYVVSPYIAEWSNTITNSIFLMTAFYSTYSAWRNKLETRYILI 71

Query: 65  HVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYS-------------PDWH 124
            +   ++ IGS L+H TLQ   Q  DE PM++  ++  + +++              +  
Sbjct: 72  GMGFSLVGIGSWLFHMTLQYRYQLLDELPMLYATIIPSWSIFAETQEILIKDEKKRKESS 131

Query: 125 YRSTMPTFLFLYGAAFAVA--HAIIRFGAGFKVHYAILCLICIPRM-YKYYIHTNDVHAK 184
           +R  M     + G    +   + +++  A F+V Y IL L+ +    +  Y H +D  AK
Sbjct: 132 FRIQMVISFIMCGIVTILTWIYVVVQKPAIFQVLYGILTLLVVVLSGWLTYYHVHDSFAK 191

Query: 185 RLAKLYLTTIS------LGTVCWLFDCLYCRNISSWYF-------NPQG-----HALWHL 244
           +   L++T +       +G +CW  D   C   S W +        P G     HA WHL
Sbjct: 192 K--NLFITMVMGMIPFVIGFICWQLDIHLC---SFWIYIRRTYLALPLGVLLELHAWWHL 251

[Close]

BLAST of CmoCh06G012460 vs. ExPASy Swiss-Prot
Match: Q6TMJ1 (Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum OX=44689 GN=dcd3A PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.9e-16
Identity = 71/278 (25.54%), Postives = 119/278 (42.81%), Query Frame = 0


Query: 3   ETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGL-------------- 62
           E   ++  P  S +WCE NY YS YIAEF+NT S++   +    G+              
Sbjct: 5   ENQGAYGTPTASIDWCELNYTYSPYIAEFYNTFSSLIISLFGIYGIWIMMPNFGTGVEKE 64

Query: 63  -VNALRQRFEKRFSVL-HVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYS 122
            +  L+Q   +   +L ++S +++ +GS  YHATL    Q  DE PM++  L+ +YI+ +
Sbjct: 65  HIKILKQLDVRNKVILSYISLIVVGVGSAFYHATLLYQNQLFDELPMIYTALIMLYIMVT 124

Query: 123 PDWH---------------YRSTMPTFLFLYGAAFAVA-------HAIIRFGAGFKVHYA 182
                               R  +P  L  YG    +          I++   G  V Y 
Sbjct: 125 VGEEKTKKGFKGGVLGNSLLRHLLPYLLIAYGLFVTITILVIQDQPKILQVSFGALVFYV 184

Query: 183 ILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLGTVCWLFDCLYCRNISSWYFNPQGH 242
           +   I +    K     ++  +  L K    ++ +G  CW+ +  +C+N  ++ F  Q H
Sbjct: 185 VFHSIYLINKKKPDGMPSNPDS-YLYKYAFVSMLVGFTCWVVERYFCKNGKTFGF--QLH 244

[Close]

BLAST of CmoCh06G012460 vs. ExPASy TrEMBL
Match: A0A6J1FQF1 (alkaline ceramidase-like OS=Cucurbita moschata OX=3662 GN=LOC111446309 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 1.7e-148
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF 60
           MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF
Sbjct: 1   MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF 60

Query: 61  SVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120
           SVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF
Sbjct: 61  SVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120

Query: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG 180
           LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG
Sbjct: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG 180

Query: 181 TVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL 240
           TVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL
Sbjct: 181 TVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL 240

Query: 241 GLFPYVKIQKPKSQ 255
           GLFPYVKIQKPKSQ
Sbjct: 241 GLFPYVKIQKPKSQ 254

[Close]

BLAST of CmoCh06G012460 vs. ExPASy TrEMBL
Match: A0A6J1ICY0 (alkaline ceramidase-like OS=Cucurbita maxima OX=3661 GN=LOC111473853 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 4.1e-147
Identity = 252/254 (99.21%), Postives = 253/254 (99.61%), Query Frame = 0


Query: 1   MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF 60
           MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF
Sbjct: 1   MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF 60

Query: 61  SVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120
           SVLH+SNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF
Sbjct: 61  SVLHISNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120

Query: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG 180
           LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG
Sbjct: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG 180

Query: 181 TVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL 240
           TV WLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL
Sbjct: 181 TVFWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL 240

Query: 241 GLFPYVKIQKPKSQ 255
           GLFPYVKIQKPKSQ
Sbjct: 241 GLFPYVKIQKPKSQ 254

[Close]

BLAST of CmoCh06G012460 vs. ExPASy TrEMBL
Match: A0A0A0L9D8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G130320 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 4.7e-143
Identity = 242/254 (95.28%), Postives = 249/254 (98.03%), Query Frame = 0


Query: 1   MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF 60
           MA+TISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILA +GLVNALRQRFEKRF
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60

Query: 61  SVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120
           SVLH+SNM+LAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF
Sbjct: 61  SVLHISNMVLAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120

Query: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG 180
           LYGAAFAVAHAIIRFGAGFKVHYAILCL+CIPRMYKYYIHTNDVHAKRLAKLYLTTISLG
Sbjct: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKYYIHTNDVHAKRLAKLYLTTISLG 180

Query: 181 TVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL 240
           TVCWLFD LYCR ISSWYFNPQGHALWH+ MGFNSYFANTFLMFCRAQQLEWNP VVHFL
Sbjct: 181 TVCWLFDRLYCRKISSWYFNPQGHALWHVLMGFNSYFANTFLMFCRAQQLEWNPRVVHFL 240

Query: 241 GLFPYVKIQKPKSQ 255
           GLFPYVK+QKPKSQ
Sbjct: 241 GLFPYVKVQKPKSQ 254

[Close]

BLAST of CmoCh06G012460 vs. ExPASy TrEMBL
Match: A0A6J1CZW2 (alkaline ceramidase-like OS=Momordica charantia OX=3673 GN=LOC111016080 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.8e-142
Identity = 240/254 (94.49%), Postives = 248/254 (97.64%), Query Frame = 0


Query: 1   MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF 60
           MA+TISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILA +GLVNALRQRFEKRF
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60

Query: 61  SVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120
           SVLH+SNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF
Sbjct: 61  SVLHISNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120

Query: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG 180
           LYGAAFAV HAIIRFG GFKVHYAILCL+CIPRMYKYYIHTND+HAKRLAKLYLTTISLG
Sbjct: 121 LYGAAFAVVHAIIRFGVGFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLG 180

Query: 181 TVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL 240
           TVCWLFD LYCRNISSWYFNPQGHALWH+ MGFNSYFANTFLMFCRAQQLEWNP VVHFL
Sbjct: 181 TVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYFANTFLMFCRAQQLEWNPRVVHFL 240

Query: 241 GLFPYVKIQKPKSQ 255
           GLFPYVK+QKPK+Q
Sbjct: 241 GLFPYVKVQKPKNQ 254

[Close]

BLAST of CmoCh06G012460 vs. ExPASy TrEMBL
Match: A0A1S3AWT6 (alkaline ceramidase 3-like OS=Cucumis melo OX=3656 GN=LOC103483475 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.3e-142
Identity = 241/254 (94.88%), Postives = 248/254 (97.64%), Query Frame = 0


Query: 1   MAETISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKRF 60
           MA+TISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILA +GLVNALRQRFEKRF
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60

Query: 61  SVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120
           SVLH+SNM+LAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF
Sbjct: 61  SVLHISNMVLAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF 120

Query: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISLG 180
           LYGAAFAVAHAIIRFGAGFKVHYAILCL+CIPRMYKYYIHTND+HAKRLAKLYL TISLG
Sbjct: 121 LYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLMTISLG 180

Query: 181 TVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHFL 240
           TVCWLFD LYCR ISSWYFNPQGHALWH+ MGFNSYFANTFLMFCRAQQLEWNP VVHFL
Sbjct: 181 TVCWLFDRLYCRKISSWYFNPQGHALWHVLMGFNSYFANTFLMFCRAQQLEWNPRVVHFL 240

Query: 241 GLFPYVKIQKPKSQ 255
           GLFPYVKIQKPKSQ
Sbjct: 241 GLFPYVKIQKPKSQ 254

[Close]

BLAST of CmoCh06G012460 vs. TAIR 10
Match: AT4G22330.1 (Alkaline phytoceramidase (aPHC) )

HSP 1 Score: 421.0 bits (1081), Expect = 6.8e-118
Identity = 194/255 (76.08%), Postives = 221/255 (86.67%), Query Frame = 0


Query: 1   MAETISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILAFVGLVNALRQRFEKR 60
           MA+ ISSFWGPVTS  E CE NY YSSYIAEF+NT+SN+P ++LA +GLVNALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHVSNMILAIGSMLYHATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFL 120
           FS+LH+SNMILAIGSMLYHATLQ VQQQ DETPMVWE+LLY+YILYSPDWHYRSTMPTFL
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAAFAVAHAIIRFGAGFKVHYAILCLICIPRMYKYYIHTNDVHAKRLAKLYLTTISL 180
           FLYGAAFA+ HA +RFG GFKVHY ILCL+CIPRMYKYYIHT D  AKR+AK Y+ TI +
Sbjct: 121 FLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILV 180

Query: 181 GTVCWLFDCLYCRNISSWYFNPQGHALWHLFMGFNSYFANTFLMFCRAQQLEWNPSVVHF 240
           G++CW  D ++C+ IS W  NPQGHALWH+FM FNSY ANTFLMFCRAQQ  WNP V +F
Sbjct: 181 GSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYF 240

Query: 241 LGLFPYVKIQKPKSQ 255
           LG+ PYVKI+KPK+Q
Sbjct: 241 LGVLPYVKIEKPKTQ 255

The following BLAST results are available for this feature:

BLAST of CmoCh06G012460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94IB9	9.5e-117	76.08	Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1	[more]
Q9D099	1.0e-25	28.29	Alkaline ceramidase 3 OS=Mus musculus OX=10090 GN=Acer3 PE=1 SV=1	[more]
Q9NUN7	1.0e-22	27.45	Alkaline ceramidase 3 OS=Homo sapiens OX=9606 GN=ACER3 PE=1 SV=3	[more]
Q02896	1.6e-18	28.42	Alkaline ceramidase YDC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
Q6TMJ1	1.9e-16	25.54	Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum OX=44689 GN=dcd3A...	[more]

BLAST of CmoCh06G012460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQF1	1.7e-148	100.00	alkaline ceramidase-like OS=Cucurbita moschata OX=3662 GN=LOC111446309 PE=3 SV=1	[more]
A0A6J1ICY0	4.1e-147	99.21	alkaline ceramidase-like OS=Cucurbita maxima OX=3661 GN=LOC111473853 PE=3 SV=1	[more]
A0A0A0L9D8	4.7e-143	95.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G130320 PE=3 SV=1	[more]
A0A6J1CZW2	1.8e-142	94.49	alkaline ceramidase-like OS=Momordica charantia OX=3673 GN=LOC111016080 PE=3 SV=...	[more]
A0A1S3AWT6	2.3e-142	94.88	alkaline ceramidase 3-like OS=Cucumis melo OX=3656 GN=LOC103483475 PE=3 SV=1	[more]

BLAST of CmoCh06G012460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22330.1	6.8e-118	76.08	Alkaline phytoceramidase (aPHC)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008901	Alkaline ceramidase	PFAM	PF05875	Ceramidase	coord: 8..235 e-value: 8.1E-54 score: 182.8
None	No IPR available	PANTHER	PTHR46852:SF5	ALKALINE CERAMIDASE	coord: 1..254
IPR044219	Alkaline ceramidase, plant	PANTHER	PTHR46852	ALKALINE CERAMIDASE	coord: 1..254

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G012460.1	CmoCh06G012460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006914	autophagy
biological_process	GO:0006672	ceramide metabolic process
biological_process	GO:0098542	defense response to other organism
biological_process	GO:0009651	response to salt stress
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides