CmoCh06G012330 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G012330
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionheat stress transcription factor A-5-like
LocationCmo_Chr06: 9329887 .. 9333367 (+)
RNA-Seq ExpressionCmoCh06G012330
SyntenyCmoCh06G012330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAACTAGGCAAGAAGCGGGTGGAGCGAAGTCAATCTCGAAGAACCTCACCTGTCGATTACTCACCAAAAGCTCACCTTCCTCTGCGTAATCTTCATCTCTAAATCTCCTTTTCAGCACCACTTTCGGTGATCGATTCACATCCTGATCCGCCGTTGTTCACCGTTAATGGAGGCAGCTGCTGGCGGCGGAGGCACTGGCGGTGCTGGTCCAGCGCCGTTTCTCATCAAGACCTACGATATGGTCGATGAATCTGCCACCGACGAGATCGTCTCGTGGACTTCTTCGAAGAAGAGCTTTGTGGTTTGGAATCCCCCTGAATTCGCTCGCCTTCTTCTTCCTACGTTTTTCAAGCACAACAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGTAAGTTACTGGAGATTCCTCTTCCGTTTACTTCTTAGTTTTGATTTTTCGCGTTTCGGTGATTTGATTTTGTTCCTCTTGTTAACTGGCTATGGAGTAGTTCCTTATCGTGTAATCATGACTTCAGTTACTGTTTTTTTCTCTCTCTCTCTCATTTACATAGTATGCATATTCGTTTCAGCAATTCTTAATGTTTCCATATTTATGAGCTTCAATTGCTGTAACTCGTAGGGATTTAACTGTGTCATAAACTAAATTTGGTCTGTTTATGCTAATCTAGTATGCAATCTCCTAGCTTCGAGATTCTTATATCTTCGACTTCTTAGAACTGTTTTCTGCGTGGAATTTTCTACGCTCTCTCTCTAGTTGAGCAACCTACTTGAAAATAATTTTCTCTTCTCCTTGCAATTCCAATTTATTAATACGTGATTCTTGAACTCATAAAAGAATCGTCCCTTGGCTGGAAGTAGTTTGAAGGGTTCCTTTATGGTTTGCTTACCGCCGTATGAATCATTTATGTGCTTGTATGATCTTTCCATCTATCATGCTAGAATCTCTTGAATAATTGTTGCTGTTCACCTCATTTTATAAGAAAACTAAATCACTCTTTCGTTTTGAAAAATTGAAGTGATTACGGCATTAAAAAGTTGCTTAAACATGCAATTTTTGAACATTTTGTTTGCAGGGGTTTAAGAAGATTGATTCTGAGAGGTGGGAATTTGCAAATGATGATTTCATAAAGGATCAAAAGCATCTGCTGAAGAACATCCACCGTCGAAAACCAATTCATAGTCACAGTACTCCTCCAGGATCTCTTGGAGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGATAGGCTATCCCGTAAAAAAACTACACTTGAAGCCGATATCTCTAGGTTCAAACAGCAAAAATCGACTGCTAAAATTCAGTTGCAGGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCGTTTTTAGAAAAGGCTGTTCAGAACCCTTCTTTCGTTGAGCATCTTGCTCGGAGAGTCGAGTCTATGGACTTTACAGCATTTAACAAAAAGAGACGATTGCCTTCAGCTGATCATTCACAGCCAGTCCTTGAAAATAGTTTTTTGGATAACCATAGCAGTTCCAGATCTGGGTCTGGGAATATTTTTCATCAAGATTTCTCACATAAGACGAGATTAGAAACTTCGAGCGTATCGGATATTAACTTACTTTCATGTAGCACTCAGAGTTCAAATGAAGGAGGGGGAAGTTCACAAAGAAACATGTCAAAACTTGACACTAGAGGTGTCCAAGAAAGTCATCATTTTGCAGCTGAAACATTAGATCTTTCAGACACTGGGACATCATTTGTATTGAAGAGGGATTCATCTTTGTCGGGGAAATCGCCTAACGACGAGAGTCCATGTCTGCACTCACTGCAACCAAATGTTAGTTCCAAAGAAGATGGAGAGAGTCACATTTCCTGTCATTTAAATCTCACTCTTGCATCTTCTTCCCTGCGAGTCAACGACGCTGATTGTTCGGTTAGAATGCCACAACTGAGTCAGAATCTTCGAAAATCCTCAGGTTCGAAAGTAAATTCAAATGGAAAAGAATCAGACATGAGACTGTTCACTAAAAACATAAATCTCGATGAGGGAACCACTACGCTTTGTCTCAAAGAGACCTCAAGTAACAATCATGGACCTCCAGCCAACCCCGTAAGAGTCAATGATGTTTTCTGGGAACAGTTTCTTACCGAAAGACCAGGTTGTCCCGAAAGTGAAGAGGCAAGTTCTAATTACAGGGAAAATCTATACAAGGAGCCAGACGACGGGAGATCGGGCCTATCACTTTAACCAGAGAGGTAATTCCAAAGCTTTTCTCACAGTTTATTTAGATTTCTCTTTCTAATTTAGACATTCAGTTATACAATAAGTGAATTCTGAAATAGGATTTGCAGTTTCTTTTTATAGTCACTATGTGATTTGTCGAGTTACTCGTATGTCGTTACAACGAGTTCTTCCTTGTACATTACAACAGTGTATAATGTTATTCATTCATGAACGATTAGCTAGTTAAATCTACACCTTATTAAAGATCTTATCTTACCATGAATACTTCATCTGTGCTTGATGGAAATATTGCTAATGAACACACTCATAAATGAATTTATGCTGAGTTGCTTCTCCATTTGGGCATGGAGAGTGCATTGTCTATTGAATAAATTGTCTTTGAGCCTTGTCTTTGTTCATTTACTTTTTAGATATCTGTGAAGTAAAACTCATGAAACTCGTGCAGTATTAAATCTTGAATAGGTGGCTACACGACTCGAGTCCATTTTCTCTAGATCTTTCTCTATTTACATCTACACTCATGTACCATTAGATCAACCTCGGGTAGTTTTTTTTGTCTTTGCTCATTCTAGTTCTGTTTCTTTATCGAATAAAACATACTATTACTTAGTTCATTACGAATCTATTCAACTTGATGAATGATAGGAGGGCGGTGTTTATTTCTATTATCGTGAATACAACTATAGAAATTTATATACTCAAGTTGTTAGGATCAGCAACATGTTAGAATTTGATAGAATTTCAATATGATCAATTACCAATATTGAAAATATTTTATGGATAATTCAATTTTAATTTCTCCTGTGGTTGTGATTGATTACAGCTGCCTGTTTATGAACCAAATGGGAAACAGCCACAGGTTGAAAATGATAGCTGACCCTACCAAAAGAGAAACAAACTCCAATCCCTGCACCTGTTCTTCCTGTGAGTTATGCTATGTTTCTATACAAATTGGATTCTTTTTTGTTTTGTTTAAATATTTTTTTAATTATAATTGGGGGAAAAAAATGAAGAGACTATGAACTCTTTCTTGTTCTATGGGGTATGTGTTTTAAGAGAGAAATATGAAGGATTGGGCCATTGGTTTTGTATGACTAAAAGTGCTTTTTAGAGGGGAATATATTGTATGGATCGAACAAAACGTGGTTTGGATAGATTTCTTTTATAAGAATAAGTTCCAACGGCTCGAGTTTATCTTAATATAGCTACGACAAC

mRNA sequence

AAAAAAAACTAGGCAAGAAGCGGGTGGAGCGAAGTCAATCTCGAAGAACCTCACCTGTCGATTACTCACCAAAAGCTCACCTTCCTCTGCGTAATCTTCATCTCTAAATCTCCTTTTCAGCACCACTTTCGGTGATCGATTCACATCCTGATCCGCCGTTGTTCACCGTTAATGGAGGCAGCTGCTGGCGGCGGAGGCACTGGCGGTGCTGGTCCAGCGCCGTTTCTCATCAAGACCTACGATATGGTCGATGAATCTGCCACCGACGAGATCGTCTCGTGGACTTCTTCGAAGAAGAGCTTTGTGGTTTGGAATCCCCCTGAATTCGCTCGCCTTCTTCTTCCTACGTTTTTCAAGCACAACAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGGGTTTAAGAAGATTGATTCTGAGAGGTGGGAATTTGCAAATGATGATTTCATAAAGGATCAAAAGCATCTGCTGAAGAACATCCACCGTCGAAAACCAATTCATAGTCACAGTACTCCTCCAGGATCTCTTGGAGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGATAGGCTATCCCGTAAAAAAACTACACTTGAAGCCGATATCTCTAGGTTCAAACAGCAAAAATCGACTGCTAAAATTCAGTTGCAGGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCGTTTTTAGAAAAGGCTGTTCAGAACCCTTCTTTCGTTGAGCATCTTGCTCGGAGAGTCGAGTCTATGGACTTTACAGCATTTAACAAAAAGAGACGATTGCCTTCAGCTGATCATTCACAGCCAGTCCTTGAAAATAGTTTTTTGGATAACCATAGCAGTTCCAGATCTGGGTCTGGGAATATTTTTCATCAAGATTTCTCACATAAGACGAGATTAGAAACTTCGAGCGTATCGGATATTAACTTACTTTCATGTAGCACTCAGAGTTCAAATGAAGGAGGGGGAAGTTCACAAAGAAACATGTCAAAACTTGACACTAGAGGTGTCCAAGAAAGTCATCATTTTGCAGCTGAAACATTAGATCTTTCAGACACTGGGACATCATTTGTATTGAAGAGGGATTCATCTTTGTCGGGGAAATCGCCTAACGACGAGAGTCCATGTCTGCACTCACTGCAACCAAATGTTAGTTCCAAAGAAGATGGAGAGAGTCACATTTCCTGTCATTTAAATCTCACTCTTGCATCTTCTTCCCTGCGAGTCAACGACGCTGATTGTTCGGTTAGAATGCCACAACTGAGTCAGAATCTTCGAAAATCCTCAGGTTCGAAAGTAAATTCAAATGGAAAAGAATCAGACATGAGACTGTTCACTAAAAACATAAATCTCGATGAGGGAACCACTACGCTTTGTCTCAAAGAGACCTCAAGTAACAATCATGGACCTCCAGCCAACCCCGTAAGAGTCAATGATGTTTTCTGGGAACAGTTTCTTACCGAAAGACCAGGTTGTCCCGAAAGTGAAGAGGCAAGTTCTAATTACAGGGAAAATCTATACAAGGAGCCAGACGACGGGAGATCGGGCCTATCACTTTAACCAGAGAGCTGCCTGTTTATGAACCAAATGGGAAACAGCCACAGGTTGAAAATGATAGCTGACCCTACCAAAAGAGAAACAAACTCCAATCCCTGCACCTGTTCTTCCTGTGAGTTATGCTATGTTTCTATACAAATTGGATTCTTTTTTGTTTTGTTTAAATATTTTTTTAATTATAATTGGGGGAAAAAAATGAAGAGACTATGAACTCTTTCTTGTTCTATGGGGTATGTGTTTTAAGAGAGAAATATGAAGGATTGGGCCATTGGTTTTGTATGACTAAAAGTGCTTTTTAGAGGGGAATATATTGTATGGATCGAACAAAACGTGGTTTGGATAGATTTCTTTTATAAGAATAAGTTCCAACGGCTCGAGTTTATCTTAATATAGCTACGACAAC

Coding sequence (CDS)

ATGGAGGCAGCTGCTGGCGGCGGAGGCACTGGCGGTGCTGGTCCAGCGCCGTTTCTCATCAAGACCTACGATATGGTCGATGAATCTGCCACCGACGAGATCGTCTCGTGGACTTCTTCGAAGAAGAGCTTTGTGGTTTGGAATCCCCCTGAATTCGCTCGCCTTCTTCTTCCTACGTTTTTCAAGCACAACAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGGGTTTAAGAAGATTGATTCTGAGAGGTGGGAATTTGCAAATGATGATTTCATAAAGGATCAAAAGCATCTGCTGAAGAACATCCACCGTCGAAAACCAATTCATAGTCACAGTACTCCTCCAGGATCTCTTGGAGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGATAGGCTATCCCGTAAAAAAACTACACTTGAAGCCGATATCTCTAGGTTCAAACAGCAAAAATCGACTGCTAAAATTCAGTTGCAGGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCGTTTTTAGAAAAGGCTGTTCAGAACCCTTCTTTCGTTGAGCATCTTGCTCGGAGAGTCGAGTCTATGGACTTTACAGCATTTAACAAAAAGAGACGATTGCCTTCAGCTGATCATTCACAGCCAGTCCTTGAAAATAGTTTTTTGGATAACCATAGCAGTTCCAGATCTGGGTCTGGGAATATTTTTCATCAAGATTTCTCACATAAGACGAGATTAGAAACTTCGAGCGTATCGGATATTAACTTACTTTCATGTAGCACTCAGAGTTCAAATGAAGGAGGGGGAAGTTCACAAAGAAACATGTCAAAACTTGACACTAGAGGTGTCCAAGAAAGTCATCATTTTGCAGCTGAAACATTAGATCTTTCAGACACTGGGACATCATTTGTATTGAAGAGGGATTCATCTTTGTCGGGGAAATCGCCTAACGACGAGAGTCCATGTCTGCACTCACTGCAACCAAATGTTAGTTCCAAAGAAGATGGAGAGAGTCACATTTCCTGTCATTTAAATCTCACTCTTGCATCTTCTTCCCTGCGAGTCAACGACGCTGATTGTTCGGTTAGAATGCCACAACTGAGTCAGAATCTTCGAAAATCCTCAGGTTCGAAAGTAAATTCAAATGGAAAAGAATCAGACATGAGACTGTTCACTAAAAACATAAATCTCGATGAGGGAACCACTACGCTTTGTCTCAAAGAGACCTCAAGTAACAATCATGGACCTCCAGCCAACCCCGTAAGAGTCAATGATGTTTTCTGGGAACAGTTTCTTACCGAAAGACCAGGTTGTCCCGAAAGTGAAGAGGCAAGTTCTAATTACAGGGAAAATCTATACAAGGAGCCAGACGACGGGAGATCGGGCCTATCACTTTAA

Protein sequence

MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSLGDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNIFHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLDLSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRVNDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHGPPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL
Homology
BLAST of CmoCh06G012330 vs. ExPASy Swiss-Prot
Match: Q94BZ5 (Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.1e-115
Identity = 241/470 (51.28%), Postives = 317/470 (67.45%), Query Frame = 0

Query: 3   AAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFK 62
           +A+  GG G  GPAPFL+KTY+MVD+S+TD+IVSW+++  SF+VWN  EF+RLLLPT+FK
Sbjct: 9   SASVSGGEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFK 68

Query: 63  HNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSLGD 122
           HNNFSSFIRQLNTYGF+KID ERWEF NDDFIKDQKHLLKNIHRRKPIHSHS PP S  D
Sbjct: 69  HNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHSHPPASSTD 128

Query: 123 PERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLE 182
            ERA  ++++D+LSR+K  +EA + +FKQQK  AK Q +++T  V+ ME RQK LL FLE
Sbjct: 129 QERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLNFLE 188

Query: 183 KAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHS-SSRSGSGNIF 242
            A++NP+FV++  ++VE +D +A+NKKRRLP  + S+P  E+S LDN S SSR  SGNIF
Sbjct: 189 TAIRNPTFVKNFGKKVEQLDISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRESGNIF 248

Query: 243 HQDFSHKTRLETSSV-SDINLLSCSTQSSNEGGGSSQRNMSKLD---TRGVQESHHFAAE 302
           HQ+FS+K RLE S   SD+N++S S QSSNE G S +  +S  D   T   +E   FA E
Sbjct: 249 HQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLPFAPE 308

Query: 303 TLDLSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSS 362
            L+L+DTGT            +   +++  + +LQ  ++S E+ +   SCHLNLTLAS+ 
Sbjct: 309 ALELADTGT---------CPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAP 368

Query: 363 LRVNDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSN 422
           L    A       Q+++   KS     NS        + T     + G   + +  + +N
Sbjct: 369 LPDKTAS------QIAKTTLKSQELNFNS--------IETSASEKNRGRQEIAVGGSQAN 428

Query: 423 NHGPPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSG 468
                A P RVNDVFWEQFLTERPG  ++EEASS YR N Y+E ++ R+G
Sbjct: 429 ----AAPPARVNDVFWEQFLTERPGSSDNEEASSTYRGNPYEEQEEKRNG 451

BLAST of CmoCh06G012330 vs. ExPASy Swiss-Prot
Match: Q6K6S5 (Heat stress transcription factor A-5 OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 5.0e-95
Identity = 227/483 (47.00%), Postives = 295/483 (61.08%), Query Frame = 0

Query: 3   AAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWT-SSKKSFVVWNPPEFARLLLPTFF 62
           A  GG G GG GPAPFL+KTY+MVD+ +TD +VSW+ +S  SFVVWN PEFA  LLP +F
Sbjct: 7   ARGGGAGGGGGGPAPFLLKTYEMVDDPSTDAVVSWSDASDASFVVWNHPEFAARLLPAYF 66

Query: 63  KHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSLG 122
           KH+NFSSFIRQLNTYGF+KID ERWEFAN+ FIK QKHLLKNIHRRKPIHSHS PPG+L 
Sbjct: 67  KHSNFSSFIRQLNTYGFRKIDPERWEFANEYFIKGQKHLLKNIHRRKPIHSHSHPPGALP 126

Query: 123 DPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFL 182
           D ERA FEDEI+RLSR+K+ L+AD+ + KQQ+S    Q++DL  +V  ME+RQ  ++AFL
Sbjct: 127 DNERAIFEDEIERLSREKSNLQADLWKSKQQQSGTMNQIEDLERRVLGMEQRQTKMIAFL 186

Query: 183 EKAVQNPSFVEHLARRVE-SMDFT-AFNKKRRLPSADHSQPVLE-NSFLDNHSS-SRSGS 242
           ++A +NP FV  L +  E S  FT AFNKKRRLP  D+S    E  SF D+HSS S+  +
Sbjct: 187 QQASKNPQFVNKLVKMAEASSIFTDAFNKKRRLPGLDYSIENTETTSFYDDHSSTSKQET 246

Query: 243 GNIFHQDFSHKTRLE-TSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAA 302
           GN+ +Q FS K RL    ++++ N+++ STQSSNE   S      + D  G +E      
Sbjct: 247 GNLLNQHFSDKLRLGLCPAMTESNIITLSTQSSNEDNRSPHGKHPECDMMG-RECLPLVP 306

Query: 303 ETLDLSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASS 362
           + ++LSDTGTS    + S  +               P +S     E  ++CHL+LTLAS 
Sbjct: 307 QMMELSDTGTSICPSKSSCFA---------------PPISD----EGLLTCHLSLTLASC 366

Query: 363 SLRVNDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSS 422
           S+ V+           SQ L  ++G+ ++ N  E+      K+  +D        K   S
Sbjct: 367 SMDVDK----------SQGL-NANGTTID-NPTEAATATMEKDDTIDRSFDDNQKKSADS 426

Query: 423 N--------------NHGPPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPD 466
                          +  P A    VND FWEQFLTERPGC E+EEASS  R +  +E  
Sbjct: 427 RTADATTPRADARVASEAPAAPAAVVNDKFWEQFLTERPGCSETEEASSGLRTDTSREQM 457

BLAST of CmoCh06G012330 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.3e-55
Identity = 171/476 (35.92%), Postives = 247/476 (51.89%), Query Frame = 0

Query: 6   GGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHNN 65
           GGGG GG GP PFLIKTY+MV+++AT+ +VSW     SFVVWNP +F+R LLP +FKHNN
Sbjct: 9   GGGGGGGGGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNN 68

Query: 66  FSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTP---PGSLGD 125
           FSSFIRQLNTYGF+KID ERWEFAN+DFI+   HLLKNIHRRKP+HSHS      G L +
Sbjct: 69  FSSFIRQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHSLQNQINGPLAE 128

Query: 126 PERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLE 185
            ER   E+EI+RL  +K+ L AD+ R  QQ+     Q+Q +  ++ +ME+RQKN++A L 
Sbjct: 129 SERRELEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVAMEQRQKNIVASLC 188

Query: 186 KAVQNPSFVEHLARRVESMDFTAFNKKRRLPSAD---------HSQPVLENSFLDNHSSS 245
           + +Q        A     ++   F+KKRR+P  D           Q V +   +   + +
Sbjct: 189 EMLQRRGG----AVSSSLLESDHFSKKRRVPKMDLFVDDCAAGEEQKVFQFQGIGTDAPA 248

Query: 246 RS-----GSGNIFHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGV 305
                   +G  F        R+E S VS    L    Q +N+   +++  M      G 
Sbjct: 249 MPPVLPVTNGEAF-------DRVELSLVS----LEKLFQRANDACTAAE-EMYSHGHGGT 308

Query: 306 QESHHFAAETLDLSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCH 365
           + S     E ++ +   T   L+  +SL   SPN                  G +H+  H
Sbjct: 309 EPSTAICPEEMNTAPMETGIDLQLPASLHPSSPN-----------------TGNAHL--H 368

Query: 366 LNLTLASSSLRVNDADCSVRMPQLSQNL---RKSSGSKVNSNGKESDMRLFTKNINLDEG 425
           L+  L  S   V   +  + M ++ +++   R  + + VNS        + +      +G
Sbjct: 369 LSTELTESPGFVQSPE--LPMAEIREDIHVTRYPTQADVNS-------EIASSTDTSQDG 428

Query: 426 TTTLCLKETSSNNHGPPANPVRVNDVFWEQFLTERP-GCPESEEASSNYRENLYKE 461
           T+     ET + +HGP       NDVFWE+FLTE P  C +  E   + ++++  E
Sbjct: 429 TS-----ETEA-SHGP------TNDVFWERFLTETPRSCLDESERQESPKDDVKAE 428

BLAST of CmoCh06G012330 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.8e-52
Identity = 122/293 (41.64%), Postives = 178/293 (60.75%), Query Frame = 0

Query: 9   GTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHNNFSS 68
           G   +   PFL KTY+MVD+S++D IVSW+ S KSF+VWNPPEF+R LLP FFKHNNFSS
Sbjct: 7   GVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSS 66

Query: 69  FIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPP-----GSLGDP 128
           FIRQLNTYGF+K D E+WEFANDDF++ Q HL+KNIHRRKP+HSHS P        L D 
Sbjct: 67  FIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLNPLTDS 126

Query: 129 ERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLEK 188
           ER    ++I+RL+++K  L  ++ +  +++   ++Q+++L  +++ MEKRQK +++F+ +
Sbjct: 127 ERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTMVSFVSQ 186

Query: 189 AVQNPSFVEHLARRVESMDFTAFNKKRRLPSADH--SQPVLENSFLDNHSSSRSGSGNIF 248
            ++ P    +L+  V   +     +KRR P  +    +P+LE +           +    
Sbjct: 187 VLEKPGLALNLSPCVPETN----ERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSPSS 246

Query: 249 HQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHF 295
           H       +LE+S     NL+S S +S  +      R+M  LD   V ES  F
Sbjct: 247 HTREHQVEQLESSIAIWENLVSDSCESMLQ-----SRSMMTLD---VDESSTF 287

BLAST of CmoCh06G012330 vs. ExPASy Swiss-Prot
Match: P41153 (Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 5.8e-51
Identity = 112/256 (43.75%), Postives = 157/256 (61.33%), Query Frame = 0

Query: 2   EAAAGGGGTGGA----------------GPAPFLIKTYDMVDESATDEIVSWTSSKKSFV 61
           +AA G GG GGA                 P PFL+KTYDMVD+ +TD+IVSW+ +  SFV
Sbjct: 10  KAAVGDGGGGGAPMLQPAPAPAPMPSANAPPPFLVKTYDMVDDPSTDKIVSWSPTNNSFV 69

Query: 62  VWNPPEFARLLLPTFFKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIH 121
           VW+PPEFA+ LLP +FKHNNFSSF+RQLNTYGF+K+D +RWEFAN+ F++ QKHLLK+I 
Sbjct: 70  VWDPPEFAKDLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKSIS 129

Query: 122 RRKPIHSHS--------------TPPG---SLG---DPERAAFEDEIDRLSRKKTTLEAD 181
           RRKP H H+               PPG   S+G   +  +   E+E++RL R K  L  +
Sbjct: 130 RRKPAHGHAQQQQQPHGHAQQQMQPPGHSASVGACVEVGKFGLEEEVERLKRDKNVLMQE 189

Query: 182 ISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLEKAVQNPSFVEHLARRVESMD--F 220
           + R +QQ+ +   QLQ +  +++ ME RQ+ +++FL KAV +P F+    ++    +   
Sbjct: 190 LVRLRQQQQSTDNQLQGMVQRLQGMELRQQQMMSFLAKAVNSPGFLAQFVQQQNESNKRI 249

BLAST of CmoCh06G012330 vs. ExPASy TrEMBL
Match: A0A6J1G983 (heat stress transcription factor A-5-like OS=Cucurbita moschata OX=3662 GN=LOC111452123 PE=3 SV=1)

HSP 1 Score: 921.4 bits (2380), Expect = 1.5e-264
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120
           FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180
           GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF
Sbjct: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240

Query: 241 FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300
           FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD
Sbjct: 241 FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300

Query: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV
Sbjct: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360

Query: 361 NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420
           NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG
Sbjct: 361 NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420

Query: 421 PPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 471
           PPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL
Sbjct: 421 PPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 470

BLAST of CmoCh06G012330 vs. ExPASy TrEMBL
Match: A0A6J1IB49 (heat stress transcription factor A-5-like OS=Cucurbita maxima OX=3661 GN=LOC111473483 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 2.9e-255
Identity = 457/470 (97.23%), Postives = 461/470 (98.09%), Query Frame = 0

Query: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120
           FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180
            DPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF
Sbjct: 121 VDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPS DHSQP +ENSFLDNHSSSRS SGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSGDHSQPAIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300
           FHQDFSHK RLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD
Sbjct: 241 FHQDFSHKMRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300

Query: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGE+HISCHLNLTLASSSL+V
Sbjct: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGENHISCHLNLTLASSSLQV 360

Query: 361 NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420
           NDAD SVRMPQL QNLRKSSGSKV SNGKESDMRLFTKNINLDEGT+T CLKETSSNNHG
Sbjct: 361 NDADYSVRMPQLGQNLRKSSGSKV-SNGKESDMRLFTKNINLDEGTSTFCLKETSSNNHG 420

Query: 421 PPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 471
           PPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL
Sbjct: 421 PPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 469

BLAST of CmoCh06G012330 vs. ExPASy TrEMBL
Match: A0A0A0L9B5 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129630 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.9e-222
Identity = 399/464 (85.99%), Postives = 428/464 (92.24%), Query Frame = 0

Query: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAA GGGTGGAGPAPFLIKTYDMVD+S+TDEIVSWTSSKKSFVVWNPPEFARLLLPTF
Sbjct: 87  MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 146

Query: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120
           FKH+NFSSFIRQLNTYGF+KIDSE+WEFAN+DFIKDQKHLLKNIHRRKPIHSHS P GS 
Sbjct: 147 FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 206

Query: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180
            DPERAAFEDEI+RL+R+KTTLE +ISRFKQQKSTAK+QLQDLTVKVESMEKRQKNLLAF
Sbjct: 207 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 266

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAF KKRRLPSAD SQPV+ENSFLDNHSSSRS SGNI
Sbjct: 267 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 326

Query: 241 FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300
           FHQDFS K RLETS  SDINL+S STQSSNE GGSSQR +SK DTR VQE+ HFA ETLD
Sbjct: 327 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 386

Query: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSF+L+RDSSLSGKS ND+SPCLHSLQP+VSSKEDGESHISC LNLTLASSSLR+
Sbjct: 387 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 446

Query: 361 NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420
           ND  CSVRMPQL QN+RK   SKVNSNGKESD+RLFTKNINLDEG+T +C +ETS+NNHG
Sbjct: 447 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 506

Query: 421 PPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDG 465
           PPA  +R NDVFWE+ LTERPGCPESEEASSNYR N +KEPDDG
Sbjct: 507 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDG 550

BLAST of CmoCh06G012330 vs. ExPASy TrEMBL
Match: A0A1S3AWR2 (heat stress transcription factor A-5 OS=Cucumis melo OX=3656 GN=LOC103483459 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.6e-221
Identity = 398/464 (85.78%), Postives = 427/464 (92.03%), Query Frame = 0

Query: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAAGGGGTGGAGPAPFL+KTYDMVD+S+TDEIVSWTSSKKSFVVWNPPEFARLLLPTF
Sbjct: 1   MEAAAGGGGTGGAGPAPFLLKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120
           FKH+NFSSFIRQLNTYGF+KIDSE+WEFAN+DFIKDQKHLLKNIHRRKPIHSHS P GS 
Sbjct: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120

Query: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180
            DPERAAFEDEI+RLSR+K TLE +ISRFKQQKSTAK+QLQDLTVKVESMEKRQKNLLAF
Sbjct: 121 IDPERAAFEDEIERLSREKNTLEVNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAF KKRRLPSAD SQPV+ENSFLDNHSSSRS SGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300
           FHQDFS K RLETS  SDINL+S STQSSNE GGSSQR +SK DTR VQE+ HFAAETLD
Sbjct: 241 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENLHFAAETLD 300

Query: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSF+L+RDSSLSGKS ND++P LHSLQP+VSSKEDGESHISC LNLTLASSSLR+
Sbjct: 301 LSDTGTSFILRRDSSLSGKSHNDDNPRLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360

Query: 361 NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420
           ND  CSVRMPQL QN+RK   SK NSNGKESD+RLFTKNINLDEG+T +C +ETS+NNHG
Sbjct: 361 NDTACSVRMPQLGQNVRKFPDSKANSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 420

Query: 421 PPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDG 465
           PPA  +R NDVFWE+ LTERPGCPESEEASSNYR N YKEPDDG
Sbjct: 421 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPYKEPDDG 464

BLAST of CmoCh06G012330 vs. ExPASy TrEMBL
Match: A0A6J1CYL3 (heat stress transcription factor A-5 OS=Momordica charantia OX=3673 GN=LOC111015981 PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 6.1e-221
Identity = 404/474 (85.23%), Postives = 428/474 (90.30%), Query Frame = 0

Query: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAAGGGG GGAGPAPFLIKTYDMVD+SATD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60

Query: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120
           FKHNNFSSFIRQLNTYGF+KIDSERWEFAN+DFIKDQKHLLKNIHRRKPIHSHS P GSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120

Query: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180
            DPERAAFEDEI+RLSR+K TLEA+ISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLP ADH QP++ENSFLDNHSSSRS SGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300
           FHQDFSHK RLE+S+VSD+NL+SCSTQSSNE GGSSQRNMSK+DTRGVQE+ HFA ETLD
Sbjct: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300

Query: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSF+LKRDSSLSGKSPNDESP L   QP+ SSKEDGESHISCHLNLTLASSSLRV
Sbjct: 301 LSDTGTSFILKRDSSLSGKSPNDESPRLS--QPSASSKEDGESHISCHLNLTLASSSLRV 360

Query: 361 NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420
           ND  CSVRMPQL Q  RKS     +SNGKE+DMRLFTKNINLDE TT  C +E  SN+HG
Sbjct: 361 NDTACSVRMPQLGQTARKSP----DSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHG 420

Query: 421 P----PANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 471
           P     A PVR ND FWEQFLTERPGCPESEEASSNYR  LYKEPDDGRSGLSL
Sbjct: 421 PSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 468

BLAST of CmoCh06G012330 vs. TAIR 10
Match: AT4G13980.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 418.3 bits (1074), Expect = 8.1e-117
Identity = 241/470 (51.28%), Postives = 317/470 (67.45%), Query Frame = 0

Query: 3   AAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFK 62
           +A+  GG G  GPAPFL+KTY+MVD+S+TD+IVSW+++  SF+VWN  EF+RLLLPT+FK
Sbjct: 9   SASVSGGEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFK 68

Query: 63  HNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSLGD 122
           HNNFSSFIRQLNTYGF+KID ERWEF NDDFIKDQKHLLKNIHRRKPIHSHS PP S  D
Sbjct: 69  HNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHSHPPASSTD 128

Query: 123 PERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLE 182
            ERA  ++++D+LSR+K  +EA + +FKQQK  AK Q +++T  V+ ME RQK LL FLE
Sbjct: 129 QERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLNFLE 188

Query: 183 KAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHS-SSRSGSGNIF 242
            A++NP+FV++  ++VE +D +A+NKKRRLP  + S+P  E+S LDN S SSR  SGNIF
Sbjct: 189 TAIRNPTFVKNFGKKVEQLDISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRESGNIF 248

Query: 243 HQDFSHKTRLETSSV-SDINLLSCSTQSSNEGGGSSQRNMSKLD---TRGVQESHHFAAE 302
           HQ+FS+K RLE S   SD+N++S S QSSNE G S +  +S  D   T   +E   FA E
Sbjct: 249 HQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLPFAPE 308

Query: 303 TLDLSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSS 362
            L+L+DTGT            +   +++  + +LQ  ++S E+ +   SCHLNLTLAS+ 
Sbjct: 309 ALELADTGT---------CPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAP 368

Query: 363 LRVNDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSN 422
           L    A       Q+++   KS     NS        + T     + G   + +  + +N
Sbjct: 369 LPDKTAS------QIAKTTLKSQELNFNS--------IETSASEKNRGRQEIAVGGSQAN 428

Query: 423 NHGPPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSG 468
                A P RVNDVFWEQFLTERPG  ++EEASS YR N Y+E ++ R+G
Sbjct: 429 ----AAPPARVNDVFWEQFLTERPGSSDNEEASSTYRGNPYEEQEEKRNG 451

BLAST of CmoCh06G012330 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 208.4 bits (529), Expect = 1.3e-53
Identity = 122/293 (41.64%), Postives = 178/293 (60.75%), Query Frame = 0

Query: 9   GTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHNNFSS 68
           G   +   PFL KTY+MVD+S++D IVSW+ S KSF+VWNPPEF+R LLP FFKHNNFSS
Sbjct: 7   GVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSS 66

Query: 69  FIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPP-----GSLGDP 128
           FIRQLNTYGF+K D E+WEFANDDF++ Q HL+KNIHRRKP+HSHS P        L D 
Sbjct: 67  FIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLNPLTDS 126

Query: 129 ERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLEK 188
           ER    ++I+RL+++K  L  ++ +  +++   ++Q+++L  +++ MEKRQK +++F+ +
Sbjct: 127 ERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTMVSFVSQ 186

Query: 189 AVQNPSFVEHLARRVESMDFTAFNKKRRLPSADH--SQPVLENSFLDNHSSSRSGSGNIF 248
            ++ P    +L+  V   +     +KRR P  +    +P+LE +           +    
Sbjct: 187 VLEKPGLALNLSPCVPETN----ERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSPSS 246

Query: 249 HQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHF 295
           H       +LE+S     NL+S S +S  +      R+M  LD   V ES  F
Sbjct: 247 HTREHQVEQLESSIAIWENLVSDSCESMLQ-----SRSMMTLD---VDESSTF 287

BLAST of CmoCh06G012330 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 199.5 bits (506), Expect = 5.9e-51
Identity = 105/218 (48.17%), Postives = 143/218 (65.60%), Query Frame = 0

Query: 15  PAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHNNFSSFIRQLN 74
           P PFL KTYDMV++ ATD IVSW+ +  SF+VW+PPEF+R LLP +FKHNNFSSF+RQLN
Sbjct: 50  PPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLN 109

Query: 75  TYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSH----STPPG---SLGDPERAA 134
           TYGF+K+D +RWEFAN+ F++ QKHLLK I RRK +  H    S P     S G    AA
Sbjct: 110 TYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAA 169

Query: 135 -----------FEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKN 194
                       E+E+++L R K  L  ++ + +QQ+ T   +LQ L   ++ ME+RQ+ 
Sbjct: 170 LSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQ 229

Query: 195 LLAFLEKAVQNPSFVEHLARRV--ESMDFTAFNKKRRL 213
           +++FL KAVQNP+F+    ++    +M  T  NKKRRL
Sbjct: 230 IMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRL 267

BLAST of CmoCh06G012330 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 193.4 bits (490), Expect = 4.2e-49
Identity = 115/286 (40.21%), Postives = 167/286 (58.39%), Query Frame = 0

Query: 17  PFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHNNFSSFIRQLNTY 76
           PFL KTYDMVD+  T+E+VSW+S   SFVVW+ PEF+++LLP +FKHNNFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 77  GFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIH---------SHSTPPGSLGDPERAA 136
           GF+K+D +RWEFAN+ F++ +K LLK+I RRKP H           S+  G+  +  +  
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEVGKFG 146

Query: 137 FEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLEKAVQN 196
            E+E++RL R K  L  ++ R +QQ+   + QLQ++  KV+ ME+RQ+ +++FL KAVQ+
Sbjct: 147 IEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQS 206

Query: 197 PSFVEHLARRVE---SMDFTAFNKKRRLP------SADHSQPVLENSFLDNHSSSRSGSG 256
           P F+  L ++     +      NKKRRLP        D+    L    +    S    + 
Sbjct: 207 PGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNVANGLNRQIVRYQPSINEAAQ 266

Query: 257 NIFHQDFSHKTRLETSSVS---DINLLSCSTQSSNEGGGSSQRNMS 282
           N+  Q  +  T     SVS   D  LL     S++   G+    +S
Sbjct: 267 NMLRQFLNTSTSPRYESVSNNPDSFLLGDVPSSTSVDNGNPSSRVS 312

BLAST of CmoCh06G012330 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 193.4 bits (490), Expect = 4.2e-49
Identity = 115/286 (40.21%), Postives = 167/286 (58.39%), Query Frame = 0

Query: 17  PFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHNNFSSFIRQLNTY 76
           PFL KTYDMVD+  T+E+VSW+S   SFVVW+ PEF+++LLP +FKHNNFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 77  GFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIH---------SHSTPPGSLGDPERAA 136
           GF+K+D +RWEFAN+ F++ +K LLK+I RRKP H           S+  G+  +  +  
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEVGKFG 146

Query: 137 FEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAFLEKAVQN 196
            E+E++RL R K  L  ++ R +QQ+   + QLQ++  KV+ ME+RQ+ +++FL KAVQ+
Sbjct: 147 IEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQS 206

Query: 197 PSFVEHLARRVE---SMDFTAFNKKRRLP------SADHSQPVLENSFLDNHSSSRSGSG 256
           P F+  L ++     +      NKKRRLP        D+    L    +    S    + 
Sbjct: 207 PGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNVANGLNRQIVRYQPSINEAAQ 266

Query: 257 NIFHQDFSHKTRLETSSVS---DINLLSCSTQSSNEGGGSSQRNMS 282
           N+  Q  +  T     SVS   D  LL     S++   G+    +S
Sbjct: 267 NMLRQFLNTSTSPRYESVSNNPDSFLLGDVPSSTSVDNGNPSSRVS 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94BZ51.1e-11551.28Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE... [more]
Q6K6S55.0e-9547.00Heat stress transcription factor A-5 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q93VB52.3e-5535.92Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
O494031.8e-5241.64Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ... [more]
P411535.8e-5143.75Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G9831.5e-264100.00heat stress transcription factor A-5-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IB492.9e-25597.23heat stress transcription factor A-5-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0L9B51.9e-22285.99HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129630 ... [more]
A0A1S3AWR21.6e-22185.78heat stress transcription factor A-5 OS=Cucumis melo OX=3656 GN=LOC103483459 PE=... [more]
A0A6J1CYL36.1e-22185.23heat stress transcription factor A-5 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
Match NameE-valueIdentityDescription
AT4G13980.18.1e-11751.28winged-helix DNA-binding transcription factor family protein [more]
AT4G18880.11.3e-5341.64heat shock transcription factor A4A [more]
AT4G17750.15.9e-5148.17heat shock factor 1 [more]
AT5G16820.14.2e-4940.21heat shock factor 3 [more]
AT5G16820.24.2e-4940.21heat shock factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 125..173
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..342
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..340
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 440..470
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..289
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..284
NoneNo IPR availablePANTHERPTHR10015:SF377HEAT STRESS TRANSCRIPTION FACTOR A-5coord: 10..467
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 69..81
score: 76.26
coord: 18..41
score: 40.36
coord: 56..68
score: 72.97
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 14..107
e-value: 2.7E-57
score: 206.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 18..107
e-value: 9.4E-30
score: 103.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 57..81
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 13..108
e-value: 1.2E-34
score: 120.9
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 10..467
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 14..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G012330.1CmoCh06G012330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0043565 sequence-specific DNA binding