Homology
BLAST of CmoCh06G008830 vs. ExPASy Swiss-Prot
Match:
P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)
HSP 1 Score: 981.5 bits (2536), Expect = 6.8e-285
Identity = 562/921 (61.02%), Postives = 667/921 (72.42%), Query Frame = 0
Query: 1 MAVETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
M ET K P+ D D +D+ P+ FKR++ AS NQ S +++ K+S +
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60
Query: 61 SDVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTD 120
S V SPNG TT KTS +KSS + + + S ++PL +T+K D +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSS-MPSSSSKASPAKSPLRNDMPSTVK--DRSQ 120
Query: 121 SQSKHLVKAAVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSS 180
Q K++S+ +E DSEDD+PLS L GN P V + K K++
Sbjct: 121 LQ---------KDQSECKIEHE--DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK--KNN 180
Query: 181 GD-------------SDDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSG 240
GD SDD P+SS F K S +SGGNQ DEKKPL+ K+HQNG T
Sbjct: 181 GDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240
Query: 241 DKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSKLVS 300
+++ G + KRPL + S+ Q S KK K+S S T + G LVS
Sbjct: 241 NEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300
Query: 301 --VKAKQ-------EVDDDDDHIPISQRMKKVALSANKSLSSKKTVS------------- 360
+KAKQ DDDDD +PIS+R K + ++N S + K V
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360
Query: 361 KVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVK 420
VVS + K +KK K SKY S+K PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420
Query: 421 MIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
++YKGK VDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480
Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLF 540
FTPIY+W+ EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLF
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLF 540
Query: 541 RGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPI 600
RGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPI
Sbjct: 541 RGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPI 600
Query: 601 NPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVAT 660
NPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVAT
Sbjct: 601 NPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVAT 660
Query: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVEL 720
YLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE
Sbjct: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEP 720
Query: 721 PVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRG 780
V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++
Sbjct: 721 LVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQE 780
Query: 781 TKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLD 840
TKDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LD
Sbjct: 781 TKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLD 840
Query: 841 RAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKIN 864
RAKKGKPPL+ +DGK+ R+L P EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKIN
Sbjct: 841 RAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKIN 892
BLAST of CmoCh06G008830 vs. ExPASy Swiss-Prot
Match:
Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)
HSP 1 Score: 968.4 bits (2502), Expect = 5.9e-281
Identity = 548/913 (60.02%), Postives = 665/913 (72.84%), Query Frame = 0
Query: 1 MAVETFGKAPM------FDEDDEDNMPIVFKRSS--ASRQNQLDSEAKQRLNKLSGRQGS 60
MA E F K + ++++DED++P+VFKR+S A+ N+ N G S
Sbjct: 1 MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIGSTKS 60
Query: 61 DVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDS 120
P + NR+ S S +SS + S+SS+Q +KSP L+ S +
Sbjct: 61 S--PPMRSPLTSPNRSAS----SSTRSSMMKPALPSSSSVQRSTLKSP---LRDDRSVVA 120
Query: 121 QSKH-LVKAAVKEESDRHPTNETSDSEDDKPLSFRLKGNS-------NPPGKGLVPTVLK 180
+ ++ KA +SD DSEDDKPLS RLK +S + G+G ++
Sbjct: 121 KERNGFGKAPSVSKSD------DEDSEDDKPLSARLKLDSKEVTKQPSSSGRGSTQQAVQ 180
Query: 181 NKVEKSSGDS--------DDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGF- 240
+ G S D+ P+SS K++ S ++K+PL++ + +NG
Sbjct: 181 KSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNIDRNGLK 240
Query: 241 --TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------- 300
T G Q P +KRPL SS Q S K+PKLS+ + P+
Sbjct: 241 PKTEGHSSQAP----AKRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHISATQDAK 300
Query: 301 GSKLVSVK--------AKQEVDDDDDHIPISQRMKKVALSANKSLSSKKTVSKVVSSSMK 360
G L + K K++ D DDH+PI+ RMK + S NKS S+K + SK+++SS +
Sbjct: 301 GKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKSDS-SNNKSSSAKPSSSKMIASSSR 360
Query: 361 KTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIYKGKLV 420
+K K VK SKY KS+K LP SGDGQKKW +L HNGVIFPPPY+ HGVK++++GK V
Sbjct: 361 TIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHGVKILFQGKPV 420
Query: 421 DLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWY 480
DLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDDCDFTPIY+WY
Sbjct: 421 DLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFTPIYEWY 480
Query: 481 QTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHPK 540
EKE KKQMT EEK+ +KEEK+KQEEKYMWA+LDGV+E++GNFRVEPPGLFRGRGEHPK
Sbjct: 481 MQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRGRGEHPK 540
Query: 541 MGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYV 600
MGKLK+RI P DITINIGK+APIPECPIPGERWKEV+HDNTVTWLAFW+DPINPKEFKYV
Sbjct: 541 MGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYV 600
Query: 601 FLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLAL 660
FLAASSSLKGQSDKEKYEKARKL ++I +IR AYTKDF +KD T+RQIAVATYLIDKLAL
Sbjct: 601 FLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLAL 660
Query: 661 RAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQ 720
RAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE V+KAI Q
Sbjct: 661 RAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQ 720
Query: 721 FQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAE 780
FQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T+DGDV E
Sbjct: 721 FQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVPE 780
Query: 781 KIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPP 840
K+V+YQ ANKEVAIICNHQRTVSKSHGAQ+ +L KI ELR+ +KEL IDLDRAKKG+ P
Sbjct: 781 KVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRTP 840
Query: 841 LKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRITV 864
L +DGK+KRNLTPE LEKKI QT KIEK+ERDMQTKED+KTVALGTSKINY+DPRITV
Sbjct: 841 LMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITV 890
BLAST of CmoCh06G008830 vs. ExPASy Swiss-Prot
Match:
P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)
HSP 1 Score: 799.7 bits (2064), Expect = 3.7e-230
Identity = 469/804 (58.33%), Postives = 565/804 (70.27%), Query Frame = 0
Query: 99 SPGTTLKVSDSTDSQSKHLVKAAVKEESDRHPTNETSDSEDDKPLSFR-----LKGNSNP 158
+PG T+ ++++ K L KEE +E DS+DDKPLS + L GNS+
Sbjct: 4 NPGITVIKQNASNVMEKTL-----KEEGQSRSNSE--DSDDDKPLSHKLSSGALNGNSHH 63
Query: 159 PGKG---LVPTVLKNKVEKSSGDSDDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKV 218
G P+ + + +D + LSS+ + + + S DE KPL S
Sbjct: 64 IRMGSNLSCPSPYTSPKPRIIKGPEDEMLLSSELQLNAGA--SNAESSDSDESKPLAS-- 123
Query: 219 HQNGFTSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQE 278
+L+ P +KRPL +S SKK KLS+ S P KL + + ++
Sbjct: 124 ---------ELEDPSSSLNKRPL--IQPKNSDPSPSKKAKLSEPSAPTNRKLKTAEQEEA 183
Query: 279 VDDDDDHIPISQRMKKVALSANKSLSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTK 338
DDD IS R KK A+K KK VS+S+ K V SK +K
Sbjct: 184 AADDDP--SISNRNKKSTTPASKVSDKKKRPD--VSASVNK--------VDFSKSLKVPL 243
Query: 339 LLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTD 398
+L +GQ LVHNGVIFPP Y+ HGVKM+Y+ VDLTPEQEEVATMFAVM +T+
Sbjct: 244 VLVKGRNGQ----PLVHNGVIFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETE 303
Query: 399 YMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKE 458
YM KPKF+ENF +DWRK+LG+ H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+KE
Sbjct: 304 YMTKPKFRENFMSDWRKILGEKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKE 363
Query: 459 EKMK-QEEKYMWAILDGV-KEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPK-DITINI 518
K + ++EKYMWA++DG+ +EKVGNFRV + + K ++K+ ++ + DITINI
Sbjct: 364 GKDETRKEKYMWAVVDGMSREKVGNFRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINI 423
Query: 519 GKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKY 578
GKDAPIPE PIPGERWKE+RHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKG+ +EKY
Sbjct: 424 GKDAPIPEPPIPGERWKEIRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKY 483
Query: 579 EKARKLKDYIKNIR-NAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGC 638
EK+RKLKDYI+ IR AYTKDF SKD +RQIAVATYLIDKLALRAGNEKDDDEADTVGC
Sbjct: 484 EKSRKLKDYIEGIRAAAYTKDFASKDSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGC 543
Query: 639 CTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLD 698
CTLKVENV+ PN+L+F+FLGKDSI+Y+N VEVE VF AI QF+SGK G DDLFD+LD
Sbjct: 544 CTLKVENVETKRPNILKFDFLGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLD 603
Query: 699 TSKLNAHLKDLMPGLTAKVFRTYNASITLDDM-----LNRGTKDGDVAEKIVIYQHANKE 758
TSKLNAHLK+LMPGLTAKVFRTYNASITLD+M L+R TK GDVAEK+V+YQHANKE
Sbjct: 604 TSKLNAHLKELMPGLTAKVFRTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKE 663
Query: 759 VAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPPLKDADGKQKRN 818
VAIICNHQRTVSKSH AQM RLNEKI EL+ +LKEL+ DL R KGKPPLK++DGK KRN
Sbjct: 664 VAIICNHQRTVSKSHSAQMVRLNEKIEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRN 723
Query: 819 LTPE----------------------VLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 864
L + + KKI+QTN KIEK+ERD +TKEDLKTVALGTS
Sbjct: 724 LNLKRYKGKLLKLIQKLRKWSETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTS 769
BLAST of CmoCh06G008830 vs. ExPASy Swiss-Prot
Match:
P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)
HSP 1 Score: 506.5 bits (1303), Expect = 6.4e-142
Identity = 338/851 (39.72%), Postives = 473/851 (55.58%), Query Frame = 0
Query: 107 SDSTDSQSKHLVKAAVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNK 166
SDS + + + ++E SDS ++ PLS L NK
Sbjct: 6 SDSVSLSIRRRQRRGSSKRISMKESDEESDSSENHPLSESL-----------------NK 65
Query: 167 VEKSSGDSDDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGV 226
KS D DD IP+ + +++S+ + N KK +KV NG G+
Sbjct: 66 KSKSESDEDD-IPIRKR---RASSKKNMSNS---SSKKR--AKVMGNG----------GL 125
Query: 227 ISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKA-KQEVDDDDDHIPISQRMK 286
+ K+ + + + PK S GSK + A K+E D DD +P+ + +
Sbjct: 126 KNGKKTAVVKEEEDFNEIAKPSPKHKRVSKANGSKNGAKSAVKKEESDTDDSVPL-RAVS 185
Query: 287 KVALSANKSLSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSL 346
V+L+ KS + S++ ++ + + K+ S + D +KWT+L
Sbjct: 186 TVSLTPYKS-----ELPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWTTL 245
Query: 347 VHNGVIFPPPYQ--THGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWN 406
HNGVIF PPY+ VK+IY G V+L PE EEVA +A M +TD+ + P F++NF+
Sbjct: 246 EHNGVIFAPPYEPLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFR 305
Query: 407 DWRKLLGK---NHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYM 466
D+ K+ + NH I+ CDFT ++ ++ ++E+KK M E+KKA+K++K ++EEKY
Sbjct: 306 DFLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYK 365
Query: 467 WAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPG 526
W ILDG KEKVGNFR+EPPGLFRGRG HPK G LKRR++P+ ITINIG+ P+PE P+PG
Sbjct: 366 WCILDGRKEKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PLPG 425
Query: 527 ERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNI 586
+W EV+HDNTVTWLA W++ IN KYVFLAA SSLKGQSD +KYEK+RKLKDYI +I
Sbjct: 426 HQWAEVKHDNTVTWLATWHENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDI 485
Query: 587 RNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPN 646
R Y KD S+ RQ A YLID ALRAGNEK +DEADTVGCC+L+ E+V PP
Sbjct: 486 RKGYRKDLKSELTVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPPR 545
Query: 647 LLEFNFLGKDSIKYENTVEVELPVFKAITQF-QSGKSGTDDLFDKLDTSKLNAHLKDLMP 706
+ F+FLGKDSI+Y N VEV+ VFK + F + K D +FD+L T+ LN +L LM
Sbjct: 546 TVVFDFLGKDSIRYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLMD 605
Query: 707 GLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQ 766
GL+AKVFRTYNAS T+ + L + K+ +A+KI+ Y AN+ VAI+CNHQR+V+K+H Q
Sbjct: 606 GLSAKVFRTYNASYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHDVQ 665
Query: 767 MSRLNEKISEL---RDILKELKIDLD-RAKKGKPPL------------------------ 826
M R E+I L R L+++ ++L+ + K KP L
Sbjct: 666 MERFAERIKALQYQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYELEK 725
Query: 827 -----------------------------------------KDADGKQKR----NLTPEV 878
DA+ K K+ + E
Sbjct: 726 EKIKKKFDRENEKLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSMEQ 785
BLAST of CmoCh06G008830 vs. ExPASy Swiss-Prot
Match:
Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)
HSP 1 Score: 498.4 bits (1282), Expect = 1.7e-139
Identity = 341/876 (38.93%), Postives = 488/876 (55.71%), Query Frame = 0
Query: 53 DVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQK---ASNSSIQAPLVKSPGTTLKVSDS 112
D SP + + S+ K +P T K A N P K P + SDS
Sbjct: 40 DEASPPQPNKKLKAENGSIKKLTPTPKPAPVTPKPTAAVNGKKPIP-KKKPVDSSSDSDS 99
Query: 113 TDSQSKHLVKAAVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGK---GLVPTVLKNK 172
+DS S +SD +++SDS+ D S +S+ P K +P
Sbjct: 100 SDSSS----------DSD----SDSSDSDSDSSSSDSSSSDSDVPKKKPISKIPNKPSGT 159
Query: 173 VEKSSGDSDDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGV 232
V K+S +S P ++ +T+ V + KP KV +S D
Sbjct: 160 VSKTSKNS----PKTTTTTTTTTTTV---------KSKP-APKVKPEQSSSDDSSSDSSD 219
Query: 233 ISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRM-- 292
SS DS S +KPK S P K + K+E P+ + +
Sbjct: 220 DSSD------DSSSDSSSEEEKPKKKPSKKPAAVK-KEITVKKETTTTTKKTPVKKEVEV 279
Query: 293 -KKVALSANKSL---------SSKKTVSKVVSSSMKKTLKKSKK-PVKKSKYVKSTKLLP 352
K+ + K + +KT +K +++ K+ LK+ ++ ++ + V
Sbjct: 280 KKETKTTTTKKVKKEESDDDGEDEKTTTKKKTTTRKRKLKEEEEGEEEEEEGVNKWWDKE 339
Query: 353 SSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQ 412
D KWT+L+HNG +FPP Y HG+K +Y GK+V LTP+QEEVAT +A +TD+++
Sbjct: 340 DDEDDGTKWTTLIHNGPVFPPAYVPHGIKFMYDGKVVKLTPKQEEVATFYANYLETDHVK 399
Query: 413 KPKFKENFWNDWRKLL--GKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEE 472
K FK+NF+ +++ LL + +++ +D CDF+ I+ +T+KE++K + EEK A E
Sbjct: 400 KQAFKDNFFKEFKGLLTSEQKEIVKKMDKCDFSHIHTHLKTKKEQRKARSKEEKDAETAE 459
Query: 473 KMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDA 532
K K +EK+ +A +DG KEKVGN+ +EPPGLF GRG+HPK G LK+RI P D+T+NIG+
Sbjct: 460 KKKIQEKHGFATIDGFKEKVGNYMIEPPGLFLGRGQHPKTGMLKQRIMPSDVTLNIGEGE 519
Query: 533 PIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR 592
IP PIPGE+WKE+ H+NTVTWLAFW + +N FKYV+LA SS +KGQ+D++K+E AR
Sbjct: 520 VIPPSPIPGEKWKEIVHNNTVTWLAFWRESVN-GGFKYVWLAPSSKIKGQADRKKFEVAR 579
Query: 593 KLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKV 652
LK+ I +IR Y K F + +RQ+AVA YLID LALR GNEK +D+ADTVGCC+L+V
Sbjct: 580 GLKNCIGDIRKGYQKSFNDESTEKRQLAVALYLIDFLALRVGNEKGEDQADTVGCCSLRV 639
Query: 653 ENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLN 712
E++K N + +FLGKDS++Y NTV++ +K + F GK TD LFD L ++LN
Sbjct: 640 EHIKLESNNTITLDFLGKDSMRYLNTVQIREDAYKVLKTFVKGKKPTDLLFDSLTVTQLN 699
Query: 713 AHLKDLMPGLTAKVFRTYNASITLDDMLNR-GTKD-GDVAEKIVIYQHANKEVAIICNHQ 772
HLK M GL+AKVFRTYNASITL L++ TK+ + EKI+ Y N+EVAI+CNHQ
Sbjct: 700 NHLKKQMTGLSAKVFRTYNASITLQKELDKMDTKEFQSIDEKILFYNRCNREVAILCNHQ 759
Query: 773 RTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPP------------------- 832
+ K+ MS+++EKI ELRD+ DL R K GK P
Sbjct: 760 KAPPKNLAETMSKIDEKIQELRDLQ-----DLCRIKIGKTPKSQQEDIESREAERLPKLI 819
Query: 833 ------LKDADGK--------------QKRNLTPEV---LEKKISQTNAKIEKIERDMQT 864
KD D + +K P+ +++KI++ +I+K E
Sbjct: 820 QLAITNKKDKDKEKTDKEISFDVKAAFEKSRTLPDAKGKIKEKIAKYEEQIKKQEILKTG 873
BLAST of CmoCh06G008830 vs. ExPASy TrEMBL
Match:
A0A6J1FIM6 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111446061 PE=3 SV=1)
HSP 1 Score: 1681.0 bits (4352), Expect = 0.0e+00
Identity = 862/863 (99.88%), Postives = 863/863 (100.00%), Query Frame = 0
Query: 1 MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ
Sbjct: 1 MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
Query: 61 SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120
SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA
Sbjct: 61 SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120
Query: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180
AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL
Sbjct: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180
Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240
SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240
Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSLSSKKT 300
SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSLSSKKT
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSLSSKKT 300
Query: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
Query: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420
VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420
Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
Query: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
Query: 841 INYLDPRITVAWCKRHEVPIEKV 864
INYLDPRITVAWCKRHEVPIEK+
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKI 863
BLAST of CmoCh06G008830 vs. ExPASy TrEMBL
Match:
A0A6J1L1R3 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111498332 PE=3 SV=1)
HSP 1 Score: 1636.7 bits (4237), Expect = 0.0e+00
Identity = 841/863 (97.45%), Postives = 851/863 (98.61%), Query Frame = 0
Query: 1 MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
MAVETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1 MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
Query: 61 SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120
+SNAQNRTTSLPKTSPMKSS+VGTQKASNSSIQA LVKSPGTTLKVSDSTDSQS+HLVKA
Sbjct: 61 TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120
Query: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180
AVKEE+DRHPTNE+SDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDD IPL
Sbjct: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180
Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240
SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNG SGDKLQKPGVISSKRPLPNADSH
Sbjct: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSH- 240
Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSLSSKKT 300
PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKKVA+SANKSLSSKKT
Sbjct: 241 LPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKT 300
Query: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
VSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
Query: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420
VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDD 420
Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
Query: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
LFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
Query: 841 INYLDPRITVAWCKRHEVPIEKV 864
INYLDPRITVAWCKRHEVPIEK+
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKI 862
BLAST of CmoCh06G008830 vs. ExPASy TrEMBL
Match:
A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)
HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 774/868 (89.17%), Postives = 808/868 (93.09%), Query Frame = 0
Query: 1 MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
MAVETFGKA MFD+DDEDNMPIVFKRSSASRQNQL+SE K QRLN+LSGR GSD CS
Sbjct: 1 MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 PNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKH 120
NGQSSNAQ R TSLPKTSPM+ E+G+QKAS SSIQ PLVKS GTTLKVSDS DS+ K
Sbjct: 61 TNGQSSNAQKRMTSLPKTSPMR-PEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVKAAVKEESDR-HPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSD 180
LV AAVKEE+D HP E SDSEDDKPLS RLKGNSN P KG +PTV KNK++KSS DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPN 240
D +PLSSKF MKS S V GG QHGFDEKKPL SKVHQNG TS DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSL 300
ADSHSS Q SSKKPKLSDSSTPL + VS+KAKQEV DDDDHIPISQRM K++ + NKS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPP 360
SKKTVSKV SSS+KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVK+++KGK VDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLK+RI P DITINIGKDAPIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTR 600
AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILK 780
DDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
Query: 781 ELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVA 840
ELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKV 864
LGTSKINYLDPRITVAWCKRHEVPIEK+
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKI 866
BLAST of CmoCh06G008830 vs. ExPASy TrEMBL
Match:
A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)
HSP 1 Score: 1489.6 bits (3855), Expect = 0.0e+00
Identity = 772/868 (88.94%), Postives = 807/868 (92.97%), Query Frame = 0
Query: 1 MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
MAVETFGKAPMFD+DDEDNMPIVFKRSSASRQNQL+SE K QRLN+LSGR GSD CS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 PNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKH 120
NGQSSNAQ R TSLPKTSPM+ E+G+QKA+ SSIQ PLVKS GTTLKVSDS DS+ K
Sbjct: 61 ANGQSSNAQKRMTSLPKTSPMR-PEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVKAAVKEESDR-HPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSD 180
L AAVKEE+D HPT E SDSEDDKPLS RLKGNSN P KG +PTV KNK++KSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPN 240
D +PLSSKF MKS S V GG QHGF EKKPL SKVHQNG TS DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSL 300
ADSHSS Q SSKKPKLSDSSTPL + VS+KAKQEV DDDDHIPISQRM K++ + NKS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPP 360
SKKTVSKV SSS+KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVK+++KGK VDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLK+RI P DITINIGKDAPIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTR 600
AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILK 780
DDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
Query: 781 ELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVA 840
ELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKV 864
LGTSKINYLDPRITVAWCKRHEVPIEK+
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKI 866
BLAST of CmoCh06G008830 vs. ExPASy TrEMBL
Match:
A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)
HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 767/874 (87.76%), Postives = 802/874 (91.76%), Query Frame = 0
Query: 1 MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
MAVETFGKAPMFD+DDEDNMPIVFKRSSASRQNQLDSE K QRLNKL GRQGSD+CS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
Query: 61 PNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSS-IQAPLVKSPGTTLKVSDSTDSQSK 120
NGQSSN Q R TSLPKTSPM+ SE+G+QKA SS IQ PL KS G +LK SDS DS+ K
Sbjct: 61 TNGQSSNNQKRMTSLPKTSPMR-SEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 HLVKAAVKEES-DRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDS 180
+LV AAVKEE+ +P+ E SDSEDDKPLS RLKGNSN P KGLV T+ KNKV+KSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLP 240
DD +PLSSKF MK S G Q GFDEKKPL+ KVHQNG TS DKLQKP V+SSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 NADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKS 300
N DSHS QFS+KKPKLSDSS+PL SKLVS+KAKQEVDDDDD IPISQR+KK + NKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 LSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPP 360
SSK TVSKVVSSS+KKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWT+LVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVKM+YKGK VDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNF 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTW 540
RVEPPGLFRGRGEHPKMGKLKRRI P DITINIGK APIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
Query: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPT 600
LAFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRNAYTKDFTSKDPT
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600
Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660
RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660
Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720
Query: 721 LDDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDIL 780
LDDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDIL
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780
Query: 781 KELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTV 840
KELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKVSSILL 869
ALGTSKINYLDPRITVAWCKRHEVPIEK + LL
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKFKNFLL 873
BLAST of CmoCh06G008830 vs. TAIR 10
Match:
AT5G55300.1 (DNA topoisomerase I alpha )
HSP 1 Score: 981.5 bits (2536), Expect = 4.8e-286
Identity = 562/921 (61.02%), Postives = 667/921 (72.42%), Query Frame = 0
Query: 1 MAVETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
M ET K P+ D D +D+ P+ FKR++ AS NQ S +++ K+S +
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60
Query: 61 SDVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTD 120
S V SPNG TT KTS +KSS + + + S ++PL +T+K D +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSS-MPSSSSKASPAKSPLRNDMPSTVK--DRSQ 120
Query: 121 SQSKHLVKAAVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSS 180
Q K++S+ +E DSEDD+PLS L GN P V + K K++
Sbjct: 121 LQ---------KDQSECKIEHE--DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK--KNN 180
Query: 181 GD-------------SDDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSG 240
GD SDD P+SS F K S +SGGNQ DEKKPL+ K+HQNG T
Sbjct: 181 GDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240
Query: 241 DKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSKLVS 300
+++ G + KRPL + S+ Q S KK K+S S T + G LVS
Sbjct: 241 NEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300
Query: 301 --VKAKQ-------EVDDDDDHIPISQRMKKVALSANKSLSSKKTVS------------- 360
+KAKQ DDDDD +PIS+R K + ++N S + K V
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360
Query: 361 KVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVK 420
VVS + K +KK K SKY S+K PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420
Query: 421 MIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
++YKGK VDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480
Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLF 540
FTPIY+W+ EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLF
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLF 540
Query: 541 RGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPI 600
RGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPI
Sbjct: 541 RGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPI 600
Query: 601 NPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVAT 660
NPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVAT
Sbjct: 601 NPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVAT 660
Query: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVEL 720
YLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE
Sbjct: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEP 720
Query: 721 PVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRG 780
V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++
Sbjct: 721 LVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQE 780
Query: 781 TKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLD 840
TKDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LD
Sbjct: 781 TKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLD 840
Query: 841 RAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKIN 864
RAKKGKPPL+ +DGK+ R+L P EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKIN
Sbjct: 841 RAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKIN 892
BLAST of CmoCh06G008830 vs. TAIR 10
Match:
AT5G55300.2 (DNA topoisomerase I alpha )
HSP 1 Score: 981.5 bits (2536), Expect = 4.8e-286
Identity = 562/921 (61.02%), Postives = 667/921 (72.42%), Query Frame = 0
Query: 1 MAVETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
M ET K P+ D D +D+ P+ FKR++ AS NQ S +++ K+S +
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60
Query: 61 SDVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTD 120
S V SPNG TT KTS +KSS + + + S ++PL +T+K D +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSS-MPSSSSKASPAKSPLRNDMPSTVK--DRSQ 120
Query: 121 SQSKHLVKAAVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSS 180
Q K++S+ +E DSEDD+PLS L GN P V + K K++
Sbjct: 121 LQ---------KDQSECKIEHE--DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK--KNN 180
Query: 181 GD-------------SDDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSG 240
GD SDD P+SS F K S +SGGNQ DEKKPL+ K+HQNG T
Sbjct: 181 GDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240
Query: 241 DKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSKLVS 300
+++ G + KRPL + S+ Q S KK K+S S T + G LVS
Sbjct: 241 NEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300
Query: 301 --VKAKQ-------EVDDDDDHIPISQRMKKVALSANKSLSSKKTVS------------- 360
+KAKQ DDDDD +PIS+R K + ++N S + K V
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360
Query: 361 KVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVK 420
VVS + K +KK K SKY S+K PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420
Query: 421 MIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
++YKGK VDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480
Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLF 540
FTPIY+W+ EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLF
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLF 540
Query: 541 RGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPI 600
RGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPI
Sbjct: 541 RGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPI 600
Query: 601 NPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVAT 660
NPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVAT
Sbjct: 601 NPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVAT 660
Query: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVEL 720
YLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE
Sbjct: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEP 720
Query: 721 PVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRG 780
V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++
Sbjct: 721 LVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQE 780
Query: 781 TKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLD 840
TKDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LD
Sbjct: 781 TKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLD 840
Query: 841 RAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKIN 864
RAKKGKPPL+ +DGK+ R+L P EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKIN
Sbjct: 841 RAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKIN 892
BLAST of CmoCh06G008830 vs. TAIR 10
Match:
AT5G55310.1 (DNA topoisomerase 1 beta )
HSP 1 Score: 968.4 bits (2502), Expect = 4.2e-282
Identity = 548/913 (60.02%), Postives = 665/913 (72.84%), Query Frame = 0
Query: 1 MAVETFGKAPM------FDEDDEDNMPIVFKRSS--ASRQNQLDSEAKQRLNKLSGRQGS 60
MA E F K + ++++DED++P+VFKR+S A+ N+ N G S
Sbjct: 1 MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIGSTKS 60
Query: 61 DVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDS 120
P + NR+ S S +SS + S+SS+Q +KSP L+ S +
Sbjct: 61 S--PPMRSPLTSPNRSAS----SSTRSSMMKPALPSSSSVQRSTLKSP---LRDDRSVVA 120
Query: 121 QSKH-LVKAAVKEESDRHPTNETSDSEDDKPLSFRLKGNS-------NPPGKGLVPTVLK 180
+ ++ KA +SD DSEDDKPLS RLK +S + G+G ++
Sbjct: 121 KERNGFGKAPSVSKSD------DEDSEDDKPLSARLKLDSKEVTKQPSSSGRGSTQQAVQ 180
Query: 181 NKVEKSSGDS--------DDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGF- 240
+ G S D+ P+SS K++ S ++K+PL++ + +NG
Sbjct: 181 KSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNIDRNGLK 240
Query: 241 --TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------- 300
T G Q P +KRPL SS Q S K+PKLS+ + P+
Sbjct: 241 PKTEGHSSQAP----AKRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHISATQDAK 300
Query: 301 GSKLVSVK--------AKQEVDDDDDHIPISQRMKKVALSANKSLSSKKTVSKVVSSSMK 360
G L + K K++ D DDH+PI+ RMK + S NKS S+K + SK+++SS +
Sbjct: 301 GKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKSDS-SNNKSSSAKPSSSKMIASSSR 360
Query: 361 KTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIYKGKLV 420
+K K VK SKY KS+K LP SGDGQKKW +L HNGVIFPPPY+ HGVK++++GK V
Sbjct: 361 TIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHGVKILFQGKPV 420
Query: 421 DLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWY 480
DLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDDCDFTPIY+WY
Sbjct: 421 DLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFTPIYEWY 480
Query: 481 QTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHPK 540
EKE KKQMT EEK+ +KEEK+KQEEKYMWA+LDGV+E++GNFRVEPPGLFRGRGEHPK
Sbjct: 481 MQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRGRGEHPK 540
Query: 541 MGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYV 600
MGKLK+RI P DITINIGK+APIPECPIPGERWKEV+HDNTVTWLAFW+DPINPKEFKYV
Sbjct: 541 MGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYV 600
Query: 601 FLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLAL 660
FLAASSSLKGQSDKEKYEKARKL ++I +IR AYTKDF +KD T+RQIAVATYLIDKLAL
Sbjct: 601 FLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLAL 660
Query: 661 RAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQ 720
RAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE V+KAI Q
Sbjct: 661 RAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQ 720
Query: 721 FQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAE 780
FQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T+DGDV E
Sbjct: 721 FQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVPE 780
Query: 781 KIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPP 840
K+V+YQ ANKEVAIICNHQRTVSKSHGAQ+ +L KI ELR+ +KEL IDLDRAKKG+ P
Sbjct: 781 KVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRTP 840
Query: 841 LKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRITV 864
L +DGK+KRNLTPE LEKKI QT KIEK+ERDMQTKED+KTVALGTSKINY+DPRITV
Sbjct: 841 LMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITV 890
BLAST of CmoCh06G008830 vs. TAIR 10
Match:
AT5G55300.3 (DNA topoisomerase I alpha )
HSP 1 Score: 390.2 bits (1001), Expect = 4.8e-108
Identity = 191/250 (76.40%), Postives = 225/250 (90.00%), Query Frame = 0
Query: 614 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 673
++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350
Query: 674 GKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIV 733
GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KIV
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410
Query: 734 IYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPPLKD 793
+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRAKKGKPPL+
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470
Query: 794 ADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRITVAWC 853
+DGK+ R+L P EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530
Query: 854 KRHEVPIEKV 864
KRHEVPIEK+
Sbjct: 531 KRHEVPIEKI 540
HSP 2 Score: 92.4 bits (228), Expect = 2.0e-18
Identity = 112/315 (35.56%), Postives = 158/315 (50.16%), Query Frame = 0
Query: 1 MAVETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
M ET K P+ D D +D+ P+ FKR++ AS NQ S +++ K+S +
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60
Query: 61 SDVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTD 120
S V SPNG TT KTS +KSS + + + S ++PL +T+K D +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSS-MPSSSSKASPAKSPLRNDMPSTVK--DRSQ 120
Query: 121 SQSKHLVKAAVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSS 180
Q K++S+ +E DSEDD+PLS L GN P V + K K++
Sbjct: 121 LQ---------KDQSECKIEHE--DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK--KNN 180
Query: 181 GD-------------SDDGIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSG 240
GD SDD P+SS F K S +SGGNQ DEKKPL+ K+HQNG T
Sbjct: 181 GDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240
Query: 241 DKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHI 294
+++ G + KRPL + S+ Q S KK K+S S T + K SV K+E+ DD +
Sbjct: 241 NEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSV--KKEI-DDKGRV 283
BLAST of CmoCh06G008830 vs. TAIR 10
Match:
AT4G26701.1 (DNA binding;DNA topoisomerase type Is )
HSP 1 Score: 94.0 bits (232), Expect = 7.0e-19
Identity = 43/47 (91.49%), Postives = 46/47 (97.87%), Query Frame = 0
Query: 817 KIEKIERDMQTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKV 864
KIEK+ERDMQTKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEK+
Sbjct: 2 KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKI 48
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| P30181 | 6.8e-285 | 61.02 | DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1 | [more] |
| Q9FJ79 | 5.9e-281 | 60.02 | DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1 | [more] |
| P93119 | 3.7e-230 | 58.33 | DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1 | [more] |
| P07799 | 6.4e-142 | 39.72 | DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... | [more] |
| Q54RC3 | 1.7e-139 | 38.93 | DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1FIM6 | 0.0e+00 | 99.88 | DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111446061 PE=3 SV=1 | [more] |
| A0A6J1L1R3 | 0.0e+00 | 97.45 | DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111498332 PE=3 SV=1 | [more] |
| A0A6J1KZE0 | 0.0e+00 | 89.17 | DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1 | [more] |
| A0A6J1EWI0 | 0.0e+00 | 88.94 | DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1 | [more] |
| A0A5A7VHI9 | 0.0e+00 | 87.76 | DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G... | [more] |
