CmoCh06G007690 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G007690
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationCmo_Chr06: 3998256 .. 4002350 (+)
RNA-Seq ExpressionCmoCh06G007690
SyntenyCmoCh06G007690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTGACGTTAACGTGTTGCGCTATCTCTCGAAAGATGATTTTAGGGTTCTTACTGCTGTTGAGATGGGGATGCGGAATGTATGTACTGATTTTCTCTTCTCTAGAGGGAACTTTAACTCATCATTTTAAGTTTATTTTGTTCCTTGAACCTTACTCTAATGTAATATCTTTTGTTGTTTCAGCATGAAATTGTTCCTTCAGAACTCGTGGAAAGTATAGCTTCTCTTAAGTATGACTTCAATTCTGATATTGTCTAGAAATATTCTTTCCCTTGCTTCTAGTAAATGTCACGTCCTACTATGGTGTGTGTAGGCATGGAGGCACTTACAAAGTTTTGAAGAATTTACTTAAATATAAGTTATTGCACCACGACTCTTCTAAATGTGAGTAGCTATTAACTTATGGTATGGGAATTCTAAATTTTAAACAATCTATCTCATTATCTTTCTTGTGTTCTTCTAGATGACAGGTTTCGACTTACATACCTTGGCTATGATTTTCTCGCAATTAAAACTTTGGTCAACTGTGGAGTGTTTGTGGCTGTAGGTCGACAAATTGGTGTTGGGAAAGAGTCAGGTAAATTTCTAGCTAGTTAGAGCTTTAAAAGTTGTTTCTATTTGTCCTGTTATTATTCATTGGCTGGGTTGCTATTGTTGATTATAGATATTTTTGAGGTTGCAAAAGAAGATGGCACTGTGCTTGCCATGAAGTTGCATAGACTTGGTAGAATTTCTTTTAGGTCGGTTAAATCTAAGCGTGATTATTTGAGGCATCGCAGTAGTTATAATTGGCTTTACTTGTCTCGGCTTGCGGCGTTTAAAGAATATGCATTCATGAAGGTGCTCTGTTGTATCGATTTTGTATTGGCATTGTATTTACGTATTTTAGTGAATTGTGATATTGATGGAGGCTTCATTGGTGATAATAGGCTTTGGAAGAACATGGTTTTCCTGTTCCAAATGCAGTAGACTGTAATAGGCATTGTGTCGTTATGTCTCTCGTTGAAGGATATCCGCTGTACGTCTTTCCTTCTTGGTTGAGTAGGGTCATGAAAGATTTTGATGAGCACGTATGCTTTAACGAATATTATGATTCTATGTCGTACCATTTCGTTCCTTCTTGGTTTATTGCAGTGTGCAGGTAAAACTATTACAAAATCCAGATGTGGTTTTTGAAACAATCATTGGTCTTGTTGTTCGACTAGCGGAACACGGACTCATTCATTGTGACTTTAACGAATTCAACATTATGGTATTCTAAGAAACAATGCTATTTACTGTTCTATTATTGTCCCTTTTCCTCGCTTAAGATGATGTCTTTTCCTCAACTTAATATATGATGTCTCTTTCTCGTCTTCATTCAATGAAACAAATTATTGCTGTTTCTCGTAAAAGGGTATTCGTGATCGTTTGTTTTTTTGAACTTACAAATATCTAATTGTAGTTCAAGTTCTCTTTTGAGCTTACCACCGTTTATTCAATCTTTCTGTTCTTGTGTTTCATAGATTGATGATGATGAGAAGGTCACAATGATCGATTTTCCACAAATGGTTTCTGTCTCGCATCGTAATGCACAAATGTAAGCGGTGCAAATATTGATTTTTCGGGTTACAATGTTTCTTGTATCATATGAATCCTAAAGTTCATGTTTCTATTAACTTATGCTTTACGTGACGATCTTTTTAAGTTTCGTTTTTCCATTTCAGGTATTTTGATCGTGAAGTTGAATGCATCTTCAAATTTTTTAGGAAAAGGTCAGTGATGTTGTAAGAAACAATAAGAATGTTTGATGTTACAAGAAGTTGAAACTTTCATGTTACATACTTCTCATGGAGTTTCTCTTCAGCTAATTTGCATGCAAGATTGAAAATTCTAAAGAACAATATAATACTGCTTTTCGAGATCGTGCAGTTATGGTCTCTGTGTTCTGGTTGCAACTTTTTACGTTCCGTTCTTAACTGGACACGATTTGTGAGGTTTAGATTAGATCGTAGCAAGTTTAATCGAAGTATGTGAACGACATGTTAGAACTCGACTAGTAGGTTTGGCTGTTATCTTGAGGTGTAGCACATTATTGATATGCTAAAGACTTTCAGTTCCTTTCTCTTACAGGTTTAACATGTCATTCCATGAGACCGTGGATGACGAAAATGATGTAGAGATCGACAATGATGAAGGTGCGAGGCCTTGCTTTTCTTCGATATCGAAAGGTGCTGGAAGTCTAGACAAGGAGCTTGCTGCCAGTGGTTTCACACGAAAGGATCAGGAAGACATTGAAAAGGTATAGAAAACCATTTGGACTTAATTGGTTAACCAGTTCATTCTAAACTTATGGGCGATTGTACGACATGCAGTTTATTGAAGGTGGAACTGAGGGAGATGCAGATTCAGGCGATGAAGAAGCTGAAAGCTTCAAGTTAGATGAAATGGACACCAATGTGGTGGAACAACAGCTACACATGTCAGACGAGGTATTCATCTCATCATTCTGATTCGAACTGATCATAGTTTGAAATTTAGGATCTAGATATTGTCCTCTTTGGGCTTTCCCTTCTAGGTTTTTAAAACGTGTTTGCTAGGGAGAAGTTTTCACACCCTTAATGTTTTGTTCTCCTCAACCAATGTGGAATCTCACAATCCACCTCCTTCGGAGCCCAGCGTCCTTGTTGGCACTTGTTCCTTTCTCCAATCGATTTGGAACCTCCCAATCCACTCCCTTCAGGGCCTAACATCATTGCTGGCACACCACCTTGTGTCCACCCACTTTCGGGGCTTAGCCTCCTTGCTGGCACATCGCCCAATGTTTGGCTTTGATACTATTTGTAACAGTCAGAGCCCATTGCTAGTACATATTGTCCTCTTTGGGCTTTCCCTTCCAAGCTTCCTCTCAAAATTTTTAAAATGCGTCTGCTGTGGAGAGGTTTGTACACCCTTATAAAGAAAACTTTGTTCTCCTTCCCAACCGATGTGAGATCTCAGAATCCACCCACTTCGAAGCCCAGCGTCCTCGCTAGCCCTGGTTCCCTTCTCCAATCGATATGGGAACCCCCAATCCACCCCTTCGGAGCCCAACGTCCTTGCTGCCACACTGCCTTGTGTCCACCTCCTTCGAAGTTCAGTCTCCTCATTGACACATTGCTCGGTGTTTGACTCTGATACTATTCGTTACAACTCAAACCCACTCTTAACAAATATTGTCATCTTTGAGCTTTCCCTTCCGGGCTTCCCCTCAAGGTTTTTAAAATTCATCTGATATGGAGAGGTTTCCACACTCTTATCCCATATTGGTTGGGGAGGAGAACGAAACACCCTTTGTAAGGGTGTGGAAACCTTCCGTAGCAGACGCGTTTTAAAGCCTTGAGGGGAAGCCTAAAGAGGACAATATCTACTAGTGGTGAATTTGGGCCGTTACAACTTCGTTTTCCTCACCAACTGACGTGGGATCTCGCAATCTTTCTTAACAACTTGAGCTCTATATAATGGATGGCACTGTTTGTTTGTTGAGAATGTAGCTGATGTACATCTATTTCCCTTTTAATTGTAATTCACAGGAAACCTTTAACTGTGATGAAAGTGCAGATTGTCAGAGGAACGAAGATTCAGATGTATTGTTTCTAACCTTGTTGGTTCGCTAATAGTTTGGTGAGCAAAGGTAACAGTTGGTATGTGTTTGTGTTTTTTCCATTGCAGAGGGAGAGTGATGGTGAGGGAAGGGATGAAAGTGATGACACAGAACTGAACAGACGGTTGAACAAATCATCATGTGGTGAACATTTGTTGTTCTAGTCAAAAGTTTTAGCCTAGGCTACAATGTCTTCTTGTGTTTCTCGTGTCGGGGATGAACTTTGTATGAACGTATTCGTATTAGTGATTAAGACACATACCTTTGATTAAGAGTTTTGAAGTTTAAATTAGTTCTATTTTCACTTAAAAAGTCTTAGTCCAATTACCCTTAGCCTTTACATCTTAATTCAACTTGAGGAATAATTTGAGACTCTAGCCAAGGCCAACCAAGTAAGTGGTTCCACCATCAGCTTGGTTGGAAGAATTATTTTATGTATGATGACATGTGCCCCTGACCCTTGCACGT

mRNA sequence

ATGAAGCTTGACGTTAACGTGTTGCGCTATCTCTCGAAAGATGATTTTAGGGTTCTTACTGCTGTTGAGATGGGGATGCGGAATCATGAAATTGTTCCTTCAGAACTCGTGGAAAGTATAGCTTCTCTTAAGCATGGAGGCACTTACAAAGTTTTGAAGAATTTACTTAAATATAAGTTATTGCACCACGACTCTTCTAAATATGACAGGTTTCGACTTACATACCTTGGCTATGATTTTCTCGCAATTAAAACTTTGGTCAACTGTGGAGTGTTTGTGGCTGTAGGTCGACAAATTGGTGTTGGGAAAGAGTCAGATATTTTTGAGGTTGCAAAAGAAGATGGCACTGTGCTTGCCATGAAGTTGCATAGACTTGGTAGAATTTCTTTTAGGTCGGTTAAATCTAAGCGTGATTATTTGAGGCATCGCAGTAGTTATAATTGGCTTTACTTGTCTCGGCTTGCGGCGTTTAAAGAATATGCATTCATGAAGGCTTTGGAAGAACATGGTTTTCCTGTTCCAAATGCAGTAGACTGTAATAGGCATTGTGTCGTTATGTCTCTCGTTGAAGGATATCCGCTTGTGCAGGTAAAACTATTACAAAATCCAGATGTGGTTTTTGAAACAATCATTGGTCTTGTTGTTCGACTAGCGGAACACGGACTCATTCATTGTGACTTTAACGAATTCAACATTATGATTGATGATGATGAGAAGGTCACAATGATCGATTTTCCACAAATGGTTTCTGTCTCGCATCGTAATGCACAAATGTATTTTGATCGTGAAGTTGAATGCATCTTCAAATTTTTTAGGAAAAGGTTTAACATGTCATTCCATGAGACCGTGGATGACGAAAATGATGTAGAGATCGACAATGATGAAGGTGCGAGGCCTTGCTTTTCTTCGATATCGAAAGGTGCTGGAAGTCTAGACAAGGAGCTTGCTGCCAGTGGTTTCACACGAAAGGATCAGGAAGACATTGAAAAGTTTATTGAAGGTGGAACTGAGGGAGATGCAGATTCAGGCGATGAAGAAGCTGAAAGCTTCAAGTTAGATGAAATGGACACCAATGTGGTGGAACAACAGCTACACATGTCAGACGAGGAAACCTTTAACTGTGATGAAAGTGCAGATTGTCAGAGGAACGAAGATTCAGATAGGGAGAGTGATGGTGAGGGAAGGGATGAAAGTGATGACACAGAACTGAACAGACGGTTGAACAAATCATCATGTGGTGAACATTTGTTGTTCTAGTCAAAAGTTTTAGCCTAGGCTACAATGTCTTCTTGTGTTTCTCGTGTCGGGGATGAACTTTGTATGAACGTATTCGTATTAGTGATTAAGACACATACCTTTGATTAAGAGTTTTGAAGTTTAAATTAGTTCTATTTTCACTTAAAAAGTCTTAGTCCAATTACCCTTAGCCTTTACATCTTAATTCAACTTGAGGAATAATTTGAGACTCTAGCCAAGGCCAACCAAGTAAGTGGTTCCACCATCAGCTTGGTTGGAAGAATTATTTTATGTATGATGACATGTGCCCCTGACCCTTGCACGT

Coding sequence (CDS)

ATGAAGCTTGACGTTAACGTGTTGCGCTATCTCTCGAAAGATGATTTTAGGGTTCTTACTGCTGTTGAGATGGGGATGCGGAATCATGAAATTGTTCCTTCAGAACTCGTGGAAAGTATAGCTTCTCTTAAGCATGGAGGCACTTACAAAGTTTTGAAGAATTTACTTAAATATAAGTTATTGCACCACGACTCTTCTAAATATGACAGGTTTCGACTTACATACCTTGGCTATGATTTTCTCGCAATTAAAACTTTGGTCAACTGTGGAGTGTTTGTGGCTGTAGGTCGACAAATTGGTGTTGGGAAAGAGTCAGATATTTTTGAGGTTGCAAAAGAAGATGGCACTGTGCTTGCCATGAAGTTGCATAGACTTGGTAGAATTTCTTTTAGGTCGGTTAAATCTAAGCGTGATTATTTGAGGCATCGCAGTAGTTATAATTGGCTTTACTTGTCTCGGCTTGCGGCGTTTAAAGAATATGCATTCATGAAGGCTTTGGAAGAACATGGTTTTCCTGTTCCAAATGCAGTAGACTGTAATAGGCATTGTGTCGTTATGTCTCTCGTTGAAGGATATCCGCTTGTGCAGGTAAAACTATTACAAAATCCAGATGTGGTTTTTGAAACAATCATTGGTCTTGTTGTTCGACTAGCGGAACACGGACTCATTCATTGTGACTTTAACGAATTCAACATTATGATTGATGATGATGAGAAGGTCACAATGATCGATTTTCCACAAATGGTTTCTGTCTCGCATCGTAATGCACAAATGTATTTTGATCGTGAAGTTGAATGCATCTTCAAATTTTTTAGGAAAAGGTTTAACATGTCATTCCATGAGACCGTGGATGACGAAAATGATGTAGAGATCGACAATGATGAAGGTGCGAGGCCTTGCTTTTCTTCGATATCGAAAGGTGCTGGAAGTCTAGACAAGGAGCTTGCTGCCAGTGGTTTCACACGAAAGGATCAGGAAGACATTGAAAAGTTTATTGAAGGTGGAACTGAGGGAGATGCAGATTCAGGCGATGAAGAAGCTGAAAGCTTCAAGTTAGATGAAATGGACACCAATGTGGTGGAACAACAGCTACACATGTCAGACGAGGAAACCTTTAACTGTGATGAAAGTGCAGATTGTCAGAGGAACGAAGATTCAGATAGGGAGAGTGATGGTGAGGGAAGGGATGAAAGTGATGACACAGAACTGAACAGACGGTTGAACAAATCATCATGTGGTGAACATTTGTTGTTCTAG

Protein sequence

MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKLLHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAMKLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCNRHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKVTMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPCFSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVVEQQLHMSDEETFNCDESADCQRNEDSDRESDGEGRDESDDTELNRRLNKSSCGEHLLF
Homology
BLAST of CmoCh06G007690 vs. ExPASy Swiss-Prot
Match: Q54T05 (Serine/threonine-protein kinase rio2 OS=Dictyostelium discoideum OX=44689 GN=rio2 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 7.5e-103
Identity = 208/417 (49.88%), Postives = 280/417 (67.15%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           MKLD   LRYLSKDDFR L AVEMGM+NHE+VP  L+ +IA+LK+GGT K ++ L K+KL
Sbjct: 1   MKLDPKALRYLSKDDFRTLVAVEMGMKNHELVPVSLICTIANLKYGGTKKSIQTLHKFKL 60

Query: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
           L HD   YD ++LTYLGYDFLA+KTLV+ GV   VG QIGVGKESDI+ VA +D   + +
Sbjct: 61  LFHDGRNYDGYKLTYLGYDFLALKTLVSRGVCSYVGNQIGVGKESDIYIVANDDNQEMVL 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180
           KLHRLGR+SF+++K+ RDYL+HR S +WLYLSRLAA KE+A+MKAL E+GFPVP  +D N
Sbjct: 121 KLHRLGRVSFKTIKNNRDYLKHRKSASWLYLSRLAALKEFAYMKALYENGFPVPTPIDYN 180

Query: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
           RHC+VMS   GYPL Q+  L++P  V+  ++ L+V+LA +GLIH DFNEFNI+I+D+E++
Sbjct: 181 RHCIVMSRARGYPLTQIVQLRHPSKVYSDLMNLIVKLASYGLIHGDFNEFNILINDEEEI 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGAR-P 300
           T+IDFPQMVS SH NA+MYFDR+V CI  FF KRFN                   G R P
Sbjct: 241 TLIDFPQMVSTSHLNAEMYFDRDVTCIRVFFEKRFNFI-----------------GERWP 300

Query: 301 CFSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNV 360
            F    +   SLD E++ASGFTR    D+EK  +  ++   D           DE++ N 
Sbjct: 301 KFKD-CQNIHSLDVEISASGFTR----DMEKEFQDLSKQQDD-----------DEINNND 360

Query: 361 VEQQLHMSDEETFNCDESADCQRNEDSDRESDGEGRDESDDTELNRRLNKSSCGEHL 417
            ++     DE+  + DE+ D   N D++ E++ E     ++ + + ++NK   G+ +
Sbjct: 361 SDENDEDDDEDDDDDDENNDDDENNDNNIENENE-----ENIKKSSKVNKKEVGKEI 379

BLAST of CmoCh06G007690 vs. ExPASy Swiss-Prot
Match: Q9CQS5 (Serine/threonine-protein kinase RIO2 OS=Mus musculus OX=10090 GN=Riok2 PE=1 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.2e-92
Identity = 196/400 (49.00%), Postives = 264/400 (66.00%), Query Frame = 0

Query: 2   KLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKLL 61
           K++V  LRY+S+DDFRVLTAVEMGM+NHEIVP  L+ SIASLKHGG  K+L+ L+K+KL+
Sbjct: 3   KVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPCSLIASIASLKHGGCNKILRELVKHKLI 62

Query: 62  HHDSSK-YDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 121
             + +K    +RLT  GYD+LA+KTL +  V  +VG Q+GVGKESDI+ VA E G  LA+
Sbjct: 63  AWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEAGQQLAL 122

Query: 122 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 181
           KLHRLGR SFR++K+KRDY +HR + +WLYLSRL+A KE+A+MKAL E  FPVP  +D N
Sbjct: 123 KLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYN 182

Query: 182 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 241
           RH V+M L+ GYPL Q+  +++P  V++  + L+V+L  HGLIH DFNEFN+M+D D+ +
Sbjct: 183 RHAVIMELINGYPLCQIHHVEDPASVYDEAMELIVKLGNHGLIHGDFNEFNLMLDKDDHI 242

Query: 242 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 301
           TMIDFPQMVS SH NA+ YFDR+V+CI +FF KRF+                 +    P 
Sbjct: 243 TMIDFPQMVSTSHPNAEWYFDRDVKCIREFFMKRFSY----------------ESELYPT 302

Query: 302 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 361
           FS I K   SLD E++ASG+T++ Q D E     G +      +E+++    DE      
Sbjct: 303 FSDIRK-EDSLDVEVSASGYTKEMQADDELLHPVGPDDKITETEEDSDFTFSDEEMLEKA 362

Query: 362 EQQLHMSDEETFNCDESAD--CQRNEDSDRESDGEGRDES 399
           +      ++E    DES    C  + DS +  DG   +ES
Sbjct: 363 KVWRSELEKEADPADESGGSWCCSSTDSKQIKDGGLPEES 385

BLAST of CmoCh06G007690 vs. ExPASy Swiss-Prot
Match: Q9BVS4 (Serine/threonine-protein kinase RIO2 OS=Homo sapiens OX=9606 GN=RIOK2 PE=1 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 3.0e-91
Identity = 195/418 (46.65%), Postives = 271/418 (64.83%), Query Frame = 0

Query: 2   KLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKLL 61
           K++V  LRY+S+DDFRVLTAVEMGM+NHEIVP  L+ SIASLKHGG  KVL+ L+K+KL+
Sbjct: 3   KVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLI 62

Query: 62  HHDSSK-YDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 121
             + +K    +RLT  GYD+LA+KTL +  V  +VG Q+GVGKESDI+ VA E+G   A+
Sbjct: 63  AWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFAL 122

Query: 122 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 181
           KLHRLGR SFR++K+KRDY +HR + +WLYLSRL+A KE+A+MKAL E  FPVP  +D N
Sbjct: 123 KLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYN 182

Query: 182 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 241
           RH VVM L+ GYPL Q+  +++P  V++  + L+V+LA HGLIH DFNEFN+++D+ + +
Sbjct: 183 RHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHI 242

Query: 242 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 301
           TMIDFPQMVS SH NA+ YFDR+V+CI  FF KRF+                 +    P 
Sbjct: 243 TMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSY----------------ESELFPT 302

Query: 302 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 361
           F  I +   +LD E++ASG+T++ Q D E     G + D +   +E   F   + +    
Sbjct: 303 FKDIRR-EDTLDVEVSASGYTKEMQADDELLHPLGPD-DKNIETKEGSEFSFSDGEVAEK 362

Query: 362 EQQLHMSDEETFNCDESAD---CQRNEDSDRESDGEGRDESDD------TELNRRLNK 410
            +     +E   NC E ++   C+ + D ++  +    +ES D      TE N+ L +
Sbjct: 363 AEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEE 402

BLAST of CmoCh06G007690 vs. ExPASy Swiss-Prot
Match: Q9P7W5 (Serine/threonine-protein kinase rio2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rio2 PE=3 SV=2)

HSP 1 Score: 310.5 bits (794), Expect = 3.0e-83
Identity = 160/357 (44.82%), Postives = 235/357 (65.83%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           + L++  +RYLS +DFR LTAVEMG RNHE+VP+ ++  IA ++ G   K L  L  +KL
Sbjct: 2   VNLNIKAMRYLSAEDFRTLTAVEMGSRNHEVVPTNMINQIAKIRGGSCTKSLSVLFMHKL 61

Query: 61  LHH-DSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLA 120
           +       Y+ +RLTY GYD+LA+K L       +VG QIGVGKESD++ V  + G    
Sbjct: 62  IAKVPHISYEGYRLTYAGYDYLALKALSKRASVYSVGNQIGVGKESDVYVVGDQKGKQYI 121

Query: 121 MKLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDC 180
           +K+HRLGRISFRSVK+ RDYLR+R + +W YLSRLAA KE+AFMK L EHGFPVP  +D 
Sbjct: 122 LKIHRLGRISFRSVKNNRDYLRNRKTGSWQYLSRLAATKEFAFMKILHEHGFPVPAPIDH 181

Query: 181 NRHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEK 240
           +RHC++M +++ +PL  V  +++P  +++T++ ++VR A +GLIH DFNEFNI++ +D  
Sbjct: 182 SRHCIIMEMIDAFPLRAVTDIRDPPALYQTLMDIIVRFARNGLIHGDFNEFNIIVREDGT 241

Query: 241 VTMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARP 300
             +IDFPQMVS SH +AQ YFDR+V+CI ++F K +                   +G  P
Sbjct: 242 AVVIDFPQMVSTSHPDAQFYFDRDVQCIVQYFEKNYQY-----------------KGDVP 301

Query: 301 CFSSISK--GAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDE 355
            F  ISK     +LD  + ASGF +K  +++EK+ +   E   ++GD+ +++++ +E
Sbjct: 302 NFEDISKMEKENNLDIMIEASGFNKKQSKELEKYRQ-EEEKRKENGDDLSDNYEEEE 340

BLAST of CmoCh06G007690 vs. ExPASy Swiss-Prot
Match: Q95Q34 (Serine/threonine-protein kinase RIO2 OS=Caenorhabditis elegans OX=6239 GN=riok-2 PE=2 SV=2)

HSP 1 Score: 289.3 bits (739), Expect = 7.1e-77
Identity = 169/407 (41.52%), Postives = 248/407 (60.93%), Query Frame = 0

Query: 2   KLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKLL 61
           +++V+++RYL  D FRVL AVEMGM+NHE+VP  LV +IA +  GG  + L +L K+ L+
Sbjct: 3   RMNVSMMRYLEGDHFRVLIAVEMGMKNHEVVPLALVSAIAGIHRGGVARTLNDLCKHSLV 62

Query: 62  HHD-SSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 121
             + S K+D +RLT  GYD+LA++ L +  V  +VG QIG+GKESD++     +   L +
Sbjct: 63  AFERSKKFDGYRLTIRGYDYLALRALCSREVVGSVGNQIGIGKESDVYVGGDPELNDLCL 122

Query: 122 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 181
           K HRLGR SFR +K KRDY + R S +WLYLSRLAA KE+AF+KAL+E GFPVP AVD  
Sbjct: 123 KFHRLGRTSFRKIKEKRDYHKKRKSASWLYLSRLAAAKEFAFLKALQERGFPVPKAVDVC 182

Query: 182 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 241
           RH VVM LV G  L  V  +++   +++ ++ L+V++A HG+IH DFNEFN+++ +DE++
Sbjct: 183 RHLVVMQLVVGQTLCNVTHVEDAGALYDRLMALIVKMARHGVIHGDFNEFNLIMLEDERI 242

Query: 242 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 301
            MIDFPQMVS+ H NA+ YFDR+V C+  FF+++F                D +    P 
Sbjct: 243 VMIDFPQMVSIDHPNAEYYFDRDVTCVRTFFKRKF----------------DYESEDWPK 302

Query: 302 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 361
           F  + +  G++D  L ASGFT+K   D+ K          D GD  A      E +    
Sbjct: 303 FDEVER-KGNMDVLLEASGFTKKMALDLNKAY--------DEGDFLAHC----EQELRNR 362

Query: 362 EQQLHMSDEETFNCDESADCQRNEDSDRESDGEGRDESDDTELNRRL 408
           +++    DE+  +  +S +  + E  D E D E     ++T   +++
Sbjct: 363 QEEDLGEDEDDSDDSKSMEDIQEEPEDLEKDHEELQAQENTVKQQKI 380

BLAST of CmoCh06G007690 vs. ExPASy TrEMBL
Match: A0A6J1FRL2 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111448172 PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.3e-235
Identity = 418/418 (100.00%), Postives = 418/418 (100.00%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60

Query: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
           LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180

Query: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
           RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV
Sbjct: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300

Query: 301 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 360
           FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 360

Query: 361 EQQLHMSDEETFNCDESADCQRNEDSDRESDGEGRDESDDTELNRRLNKSSCGEHLLF 419
           EQQLHMSDEETFNCDESADCQRNEDSDRESDGEGRDESDDTELNRRLNKSSCGEHLLF
Sbjct: 361 EQQLHMSDEETFNCDESADCQRNEDSDRESDGEGRDESDDTELNRRLNKSSCGEHLLF 418

BLAST of CmoCh06G007690 vs. ExPASy TrEMBL
Match: A0A6J1DEY7 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020422 PE=3 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.2e-198
Identity = 366/440 (83.18%), Postives = 389/440 (88.41%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELV+ IASLKHGGTYKV+KNLLKYKL
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVDRIASLKHGGTYKVMKNLLKYKL 60

Query: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
           LHHDSSKYD FRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMKALEEHGFPVPNAVDCN
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDCN 180

Query: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
           RHCV+MSLV+GYPLVQVK LQNPDVVFETI+GLVV LAEHGLIHCDFNEFNIMID+DEKV
Sbjct: 181 RHCVIMSLVQGYPLVQVKELQNPDVVFETILGLVVHLAEHGLIHCDFNEFNIMIDNDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDR+VECIFKFFRKRFN+SFHE+ DDENDVE+DNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNLSFHESGDDENDVEVDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 360
           FSSI+KGAGSLDK+LAASGFTRKDQED+EKFIEGG EGDADSGDEEAES KLD+MD NVV
Sbjct: 301 FSSIAKGAGSLDKDLAASGFTRKDQEDMEKFIEGGLEGDADSGDEEAESSKLDKMDNNVV 360

Query: 361 EQQLHMSDEETFNCDESADCQRN------------EDSDRE------------------- 410
           E  L +SDEET N DE+A+CQ N             DSD E                   
Sbjct: 361 E-HLQLSDEETLNGDENAECQSNYKARHNDVFENDSDSDNEEANGENDVSRNGSSETREN 420

BLAST of CmoCh06G007690 vs. ExPASy TrEMBL
Match: A0A6J1DH16 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020422 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.9e-198
Identity = 368/441 (83.45%), Postives = 391/441 (88.66%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELV+ IASLKHGGTYKV+KNLLKYKL
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVDRIASLKHGGTYKVMKNLLKYKL 60

Query: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
           LHHDSSKYD FRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMKALEEHGFPVPNAVDCN
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDCN 180

Query: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
           RHCV+MSLV+GYPLVQVK LQNPDVVFETI+GLVV LAEHGLIHCDFNEFNIMID+DEKV
Sbjct: 181 RHCVIMSLVQGYPLVQVKELQNPDVVFETILGLVVHLAEHGLIHCDFNEFNIMIDNDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDR+VECIFKFFRKRFN+SFHE+ DDENDVE+DNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNLSFHESGDDENDVEVDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 360
           FSSI+KGAGSLDK+LAASGFTRKDQED+EKFIEGG EGDADSGDEEAES KLD+MD NVV
Sbjct: 301 FSSIAKGAGSLDKDLAASGFTRKDQEDMEKFIEGGLEGDADSGDEEAESSKLDKMDNNVV 360

Query: 361 EQQLHMSDEETFNCDESADCQRN------------EDSD-------------------RE 410
           E  L +SDEET N DE+A+CQ N             DSD                   RE
Sbjct: 361 E-HLQLSDEETLNGDENAECQSNYKARHNDVFENDSDSDQNEEANGENDVSRNGSSETRE 420

BLAST of CmoCh06G007690 vs. ExPASy TrEMBL
Match: A0A6J1L5Z7 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111500302 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.9e-197
Identity = 370/438 (84.47%), Postives = 386/438 (88.13%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE IASL+HGGTYKVLKNLLKYKL
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
           LHHDSSKYD FRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMKALEEHGFPVPNAVD N
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
           RHCV+MSLVEGYPLVQVK LQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDR+VEC+FKFFRKRFN+SFHETVDDEND+EIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDLEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 360
           FSSISKGAGSLDKELAASGFTRK+QEDIEKFIEGG E DADSGDEEAES KL+EMDTN V
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGDEEAESSKLNEMDTNAV 360

Query: 361 EQQLHMSDEETFNCDESADCQRN----------EDSD-------------------RESD 410
           E  L +S EET + DE+ D Q N           DSD                   R+ +
Sbjct: 361 E-HLRISVEETLSGDENVDLQGNHEACLDDVSENDSDSNNQEANETGILENGSSETRDDE 420

BLAST of CmoCh06G007690 vs. ExPASy TrEMBL
Match: A0A6J1L8N7 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111500302 PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.4e-197
Identity = 370/439 (84.28%), Postives = 386/439 (87.93%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE IASL+HGGTYKVLKNLLKYKL
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
           LHHDSSKYD FRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMKALEEHGFPVPNAVD N
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
           RHCV+MSLVEGYPLVQVK LQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDR+VEC+FKFFRKRFN+SFHETVDDEND+EIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDLEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 360
           FSSISKGAGSLDKELAASGFTRK+QEDIEKFIEGG E DADSGDEEAES KL+EMDTN V
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGDEEAESSKLNEMDTNAV 360

Query: 361 EQQLHMSDEETFNCDESADCQRN----------EDSD--------------------RES 410
           E  L +S EET + DE+ D Q N           DSD                    R+ 
Sbjct: 361 E-HLRISVEETLSGDENVDLQGNHEACLDDVSENDSDSNQNQEANETGILENGSSETRDD 420

BLAST of CmoCh06G007690 vs. TAIR 10
Match: AT3G51270.1 (protein serine/threonine kinases;ATP binding;catalytics )

HSP 1 Score: 570.9 bits (1470), Expect = 8.7e-163
Identity = 302/403 (74.94%), Postives = 338/403 (83.87%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE IA LKHGGTYKVLKNLLKYKL
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIACLKHGGTYKVLKNLLKYKL 60

Query: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
           LHHDSSKYD FRLTYLGYDFLAIKTLVN G+F  VGRQIGVGKESDIFEVA+EDGT+LAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGIFTGVGRQIGVGKESDIFEVAQEDGTILAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180
           KLHRLGR SFR+VKSKRDYLRHRSS++WLYLSRLAA KE+AFMKALEEH FPVP A+DCN
Sbjct: 121 KLHRLGRTSFRAVKSKRDYLRHRSSFSWLYLSRLAALKEFAFMKALEEHDFPVPKAIDCN 180

Query: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
           RHCV+MSLV+GYP+VQVK LQNP+ +FE IIG+VVRLAEHGLIHCDFNEFNIMIDD+EK+
Sbjct: 181 RHCVIMSLVQGYPMVQVKQLQNPETIFEKIIGIVVRLAEHGLIHCDFNEFNIMIDDEEKI 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDR++ECIFKFFRKRFNMSFHE   +  + E+  DE +RP 
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDIECIFKFFRKRFNMSFHEDKGESEETEV--DENSRPS 300

Query: 301 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 360
           F  I+K A +LDK+L ASGFTRK+Q D++KFIEGG E   DS DE+ ES   D+      
Sbjct: 301 FFDITKDANALDKDLEASGFTRKEQTDLDKFIEGGVEKSEDS-DEDEES---DD------ 360

Query: 361 EQQLHMSDEETFNCDESADCQRNEDSDRESDG-EGRDESDDTE 403
           E+Q   S+EE  N +E    Q  +   + SDG E   E D+TE
Sbjct: 361 EEQTCESNEEG-NLNEIKSLQLQDKEQKSSDGVEAEVELDNTE 390

BLAST of CmoCh06G007690 vs. TAIR 10
Match: AT3G51270.2 (protein serine/threonine kinases;ATP binding;catalytics )

HSP 1 Score: 548.9 bits (1413), Expect = 3.5e-156
Identity = 296/403 (73.45%), Postives = 330/403 (81.89%), Query Frame = 0

Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVESIASLKHGGTYKVLKNLLKYKL 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE IA LKHGGTYKVLKNLLKYKL
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIACLKHGGTYKVLKNLLKYKL 60

Query: 61  LHHDSSKYDRFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
           LHHDSSKYD FRLTYLGYDFLAIKTLVN G+F  VGRQIGVGKESDIFEVA+EDGT+LAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGIFTGVGRQIGVGKESDIFEVAQEDGTILAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180
           KLHRLGR SFR+VKSKRDYLRHRSS++WLYLSRLAA KE+AFMKALEEH FPVP A+DCN
Sbjct: 121 KLHRLGRTSFRAVKSKRDYLRHRSSFSWLYLSRLAALKEFAFMKALEEHDFPVPKAIDCN 180

Query: 181 RHCVVMSLVEGYPLVQVKLLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
           RHCV+M        VQVK LQNP+ +FE IIG+VVRLAEHGLIHCDFNEFNIMIDD+EK+
Sbjct: 181 RHCVIM--------VQVKQLQNPETIFEKIIGIVVRLAEHGLIHCDFNEFNIMIDDEEKI 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDREVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDR++ECIFKFFRKRFNMSFHE   +  + E+  DE +RP 
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDIECIFKFFRKRFNMSFHEDKGESEETEV--DENSRPS 300

Query: 301 FSSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGTEGDADSGDEEAESFKLDEMDTNVV 360
           F  I+K A +LDK+L ASGFTRK+Q D++KFIEGG E   DS DE+ ES   D+      
Sbjct: 301 FFDITKDANALDKDLEASGFTRKEQTDLDKFIEGGVEKSEDS-DEDEES---DD------ 360

Query: 361 EQQLHMSDEETFNCDESADCQRNEDSDRESDG-EGRDESDDTE 403
           E+Q   S+EE  N +E    Q  +   + SDG E   E D+TE
Sbjct: 361 EEQTCESNEEG-NLNEIKSLQLQDKEQKSSDGVEAEVELDNTE 382

BLAST of CmoCh06G007690 vs. TAIR 10
Match: AT5G37350.1 (Serine/threonine-protein kinase Rio1 )

HSP 1 Score: 63.2 bits (152), Expect = 5.9e-10
Identity = 93/364 (25.55%), Postives = 157/364 (43.13%), Query Frame = 0

Query: 86  LVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAMKLHRLGRISF--RSVKSKRDYLRHR 145
           ++N GVF  V   I  GKE++++   K DG+ LA+K+++   + F  R    + DY R R
Sbjct: 157 MLNRGVFNDVNGCISTGKEANVYHATKSDGSELAIKVYKTSVLVFKDRDRYVQGDY-RFR 216

Query: 146 SSY---NWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCNRHCVVMSLV--EGYPLVQVK 205
             Y   N   + +  A KE   +K L   G   P  +    H +VM  +  +G+   ++K
Sbjct: 217 YGYCRHNPRKMVKTWAEKEQRNLKRLHAAGIRCPAVILLRLHVLVMEFIGRDGWAAPRLK 276

Query: 206 -----LLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKVTMIDFPQMVSVSH 265
                L +  +   E II + V   +  L+H D +E+NI+  +   + +ID  Q V + H
Sbjct: 277 DAALSLDKLRECYLELIIQMRVLYQKCKLVHGDLSEYNILYFEGH-LYIIDVSQSVDLDH 336

Query: 266 RNAQMYFDREVECIFKFFRKR----------FNMSFHETVDDENDVEIDNDEGARPCFSS 325
             A  +   + + +  FF+K           F+     T+ DEN V+   +E  R     
Sbjct: 337 PLALNFLREDCDHVSDFFKKHGVAVMTIRELFDFIVDPTISDEN-VDSYLEEVQR---KV 396

Query: 326 ISKGAGSLDKELAASGFTR-----------KDQEDIEKFIEGGTEGDADSGDEEAESFK- 385
           I +G  S++ E+A S F +             + D+ K   G      D+GD   ++   
Sbjct: 397 IERGEISVEDEIADSVFMKSYIPKSLDAVNNPEADVAKITSG-----QDTGDMLYQTITG 456

Query: 386 LDEMDTNVVEQQLHMSDEETFNCDESADCQRNEDSDRESDGEGRDESDDTELNRRLNKSS 416
           L +    V EQQ+ ++ EE             E+ + E  GE  +E  + EL     K++
Sbjct: 457 LKDALPKVDEQQIEVNAEE------------EEEEEEEGSGEESEEESEKELGPEDKKAA 497

BLAST of CmoCh06G007690 vs. TAIR 10
Match: AT2G24990.1 (Serine/threonine-protein kinase Rio1 )

HSP 1 Score: 62.8 bits (151), Expect = 7.7e-10
Identity = 89/356 (25.00%), Postives = 155/356 (43.54%), Query Frame = 0

Query: 86  LVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAMKLHRLGRISF--RSVKSKRDYLRHR 145
           ++N GVF  V   I  GKE++++   K DG+ LA+K+++   + F  R    + DY R R
Sbjct: 158 MLNRGVFNDVNGCISTGKEANVYHATKSDGSELAIKVYKTSVLVFKDRDRYVQGDY-RFR 217

Query: 146 SSY---NWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCNRHCVVMSLV--EGYPLVQVK 205
             Y   N   + +  A KE   +K L   G   P  +    H +VM  +  +G+   ++K
Sbjct: 218 YGYCRHNPRKMVKTWAEKEQRNLKRLHAAGIRCPAVILLRLHVLVMEFIGRDGWAAPRLK 277

Query: 206 -----LLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKVTMIDFPQMVSVSH 265
                L +  +   E II + V   +  L+H D +E+NI+  +   + +ID  Q V + H
Sbjct: 278 DAALSLDKLRECYLELIIQMRVLYQKCKLVHGDLSEYNILYFEGH-LYIIDVSQSVDLDH 337

Query: 266 RNAQMYFDREVECIFKFFRKR----------FNMSFHETVDDENDVEIDNDEGARPCFSS 325
             A  +   + + +  FF+K           F+      + DEN V+   +E  R     
Sbjct: 338 PLALNFLREDCDHVSDFFKKHGVAVMTIRELFDFIVDPAITDEN-VDSYLEEVQR---KV 397

Query: 326 ISKGAGSLDKELAASGFTR-----------KDQEDIEKFIEGGTEGDADSGDEEAESFK- 385
           I +G  S++ E+A S F +             + D+ K   G      D+GD   ++   
Sbjct: 398 IERGEISVEDEIADSVFMKSYIPKSLDAVNNPEADVAKITSG-----QDTGDMLYQTITG 457

Query: 386 LDEMDTNVVEQQLHMSDEETFNCDESADCQRNEDSDRESDGEGRDESDDTELNRRL 408
           L +    + EQ++ ++DEE           + E+ + E DGE  + S++ E    L
Sbjct: 458 LKDALPKIEEQKIEVNDEE-----------KEEEGEEEEDGESEEGSEEEESEEEL 491

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54T057.5e-10349.88Serine/threonine-protein kinase rio2 OS=Dictyostelium discoideum OX=44689 GN=rio... [more]
Q9CQS51.2e-9249.00Serine/threonine-protein kinase RIO2 OS=Mus musculus OX=10090 GN=Riok2 PE=1 SV=1[more]
Q9BVS43.0e-9146.65Serine/threonine-protein kinase RIO2 OS=Homo sapiens OX=9606 GN=RIOK2 PE=1 SV=2[more]
Q9P7W53.0e-8344.82Serine/threonine-protein kinase rio2 OS=Schizosaccharomyces pombe (strain 972 / ... [more]
Q95Q347.1e-7741.52Serine/threonine-protein kinase RIO2 OS=Caenorhabditis elegans OX=6239 GN=riok-2... [more]
Match NameE-valueIdentityDescription
A0A6J1FRL22.3e-235100.00Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DEY72.2e-19883.18Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1DH162.9e-19883.45Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1L5Z71.9e-19784.47Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1L8N72.4e-19784.28Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G51270.18.7e-16374.94protein serine/threonine kinases;ATP binding;catalytics [more]
AT3G51270.23.5e-15673.45protein serine/threonine kinases;ATP binding;catalytics [more]
AT5G37350.15.9e-1025.55Serine/threonine-protein kinase Rio1 [more]
AT2G24990.17.7e-1025.00Serine/threonine-protein kinase Rio1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000687RIO kinaseSMARTSM00090rio_2coord: 64..290
e-value: 2.7E-52
score: 189.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 192..338
e-value: 2.2E-42
score: 146.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 93..191
e-value: 1.1E-32
score: 114.2
NoneNo IPR availablePFAMPF01163RIO1coord: 106..280
e-value: 5.6E-50
score: 169.6
NoneNo IPR availablePIRSRPIRSR038147-2PIRSR038147-2coord: 85..404
e-value: 2.9E-9
score: 34.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..418
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 368..389
NoneNo IPR availablePANTHERPTHR45852:SF2BNAA01G19540D PROTEINcoord: 1..409
NoneNo IPR availablePANTHERPTHR45852SER/THR-PROTEIN KINASE RIO2coord: 1..409
IPR015285RIO2 kinase winged helix domain, N-terminalPFAMPF09202Rio2_Ncoord: 8..88
e-value: 6.8E-32
score: 109.5
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 4..90
e-value: 5.5E-34
score: 117.8
IPR030484Serine/threonine-protein kinase Rio2CDDcd05144RIO2_Ccoord: 94..274
e-value: 2.43244E-117
score: 337.94
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 95..270
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 5..88

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G007690.1CmoCh06G007690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030490 maturation of SSU-rRNA
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
cellular_component GO:0030688 preribosome, small subunit precursor
molecular_function GO:0005524 ATP binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity