CmoCh06G007360 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G007360
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptioncytochrome P450 87A3-like
LocationCmo_Chr06: 3799712 .. 3803409 (+)
RNA-Seq ExpressionCmoCh06G007360
SyntenyCmoCh06G007360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGCACATCCAAGCACCACTTACCCACAATACTTCCATTTCAGTTTGCAGCAACCAAAATGTGGGTTCTTTTGATAGGAACTATTGTTGTTTTGAGCTTTACACACTGGCTTTACCGTTGGAGAAACCCCAAATGTAATGGGAAACTCCCACCAGGTTCAATGGGGTTCCCTCTGCTTGGTGAAACCTTCCAGTTCTTCGCTCCCAATACCTCCTCTGATGTCCCTCCTTTCATCAGAAACAGAGTGGATAGGTACGGACCAATCTTCAGAACAAGTTTGGTGGGAAGACCGCTGATTGTCTCAACCGACCCCGACTTCAATCACTTCATCTTTCAACAAGAGGGTCACTTGTTCCAGAGCTGGTATCCATCTACCTTCACCCAGATATTTGGAAGGCAAAATGTTGGTTCATTGCACGGTTTCATGTATAAGTTCCTGAAGAACATGGTTCTTCATCTTGTTGGCCCTGAAAATCTGAGGAAGATGATATCTGAAGTGGAGGCTGTAGCTACCACAAGATTGACACAATGGTCATCTCTTGAATCGGTTGAATTGAAGGATGAGACTGCAAGTGTAATTACAATGAAAAGTTTAGAACCAAACCTGTTTGTTCTCCATGTTTGGTTTGATACTGATTTTTGTTGTTCTTGCAGATGATTTTTAATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTGAAGAGAATTTGAGAGACAACTTCGTTGCATTCATACAGGGCTTGATTTCCTTCCCTCTAAATGTTTTTGGAACAGCTTATAACAAATGTTTACAGGTAAACCAATTATACTACAGTCATTCTAACTATAAGCTCTCGTTGGTTGGAGAGGGGAACAAACCTCTCCCTAATAGACGATTTTAAAATTATGAGGTTGACGGCGATTTGTAATGAGCTAAAGCGGACAATATCTGTTAGTAGTAGACTTGGGGCTGTTACAAATAGTATCAGAGCTAGACATTGGGTGGTGTGCCAGTGAGGATGTTGGGCCCCAAGGGTACGGATTGTGAGTTCCCACGTCGGTTGGAGAGAGGAACAAACCTCTCCCTAGTAGACACGTTTTAAAACCGTGAGGTTGATGACGATACGTAACAAGGTGTTAGTGGTGGATTTGGGTTGTTACAAATGGTATCAGAGCTAGACATCAGGCGGTGTGCCAGTTAGGATGCTGGGCCCCAAGAGGGGTGGATTGTGAGGTCCCACGTCGGTTGGAGAGAGGAGGAACAAACCTCTCCCTAGTAGACGCGTTTTAAAAATGTGAGACGACGATACGTAACGGGCCAAAACAGATAATATTTGTTAATGGTAGACTTGGGCTGTTACAAATGGTATTAGAGCAAGACATCAGGTGGTATGCCAGCGAGAACGCTGAGCCTCAGGGGGGTGGATTGTGAGGTCCCACATCGGTTGGAGAGAGGAACAACCCTCTCTCTAGTAGACACGTTTTAAAACCATGAGGTTGACGACGATACGTAATGGGGCAAAGCGGACAATATCTATTAGCAGTGGACTTTGGCTATTAAAAATGGTATCAGAGTCAGACATCGAGCAGTGTGCCAGCGAGGATGCTGGGCCCTAAGAGGGGTGGATTGTGAGGTCCCACATCAATTGGAGAGGGGAACAAACCTCTCCCTAGTATACGGATTTTAAAACCGTAAGGTTAACGACGATACATAACGAGTCAAAACAGACAATATCTACTAGTAGTATATTTAGGCACCTCCTATAGAAACTGAGCGTGTTCTTCCCTAATTCAACAATTCAACAGGGCAGGAAAAGAGCGATGAGGATGCTGAAAAACATGCTACAAGAAAGGCGTGCAAATCCAAGGAAAGAGCAAACAGATTTTTTTGATTATGTTCTTGAAGAACTGGCGAAGGATGGAACTCTTCTCACCGAGGAGATTGCTTTGGACCTCATGTTTGTGCTGCTTTTTGCGAGCTTCGAAACTACTTCACTTGCTATTACTGCAGCCATTAAATTCCTCCTCAACAATCCTCATGTGCTGGAGGAGCTGACGGTCTGTTTAATGTTGTTTCAACTTTAAACAACAATATCCTCATGTTAAATCCATTATTTGCATCTTTATCAGTGTGAACATGAGGGGATATTGAAGGCAAGGGAGAATCCAGATTGTGGGCTTACATGGGATGAATACAAATCCATGACATTCACATTTCAGGTTACTTTAATTATTTCATCTCTTGAAGGCCTCTTTGTTTCTCATAACTAAACTTCTTCAATTGTCAGTTCATCAATGAAACTGTGAGACTGGCAAATATAGTTCCGGGAATCTTTCGAAAAGCACTGAGAGACATCCAGTTTAAGGGTATGTATGTAGTGTATGTATGTATATTGAGCTCATAGATTATTTTTCTTGATAACATCATCACAAATGGCGGCTACACATTTGTTGGAGCAGGATATACCATTCCAGCTGGGTGGTCAGTTATGGTCTGCCCTCCTGCCGTGCACTTGAATCCTGAAAAATATGTTGATCCCCTCGCTTTTAATCCATGGCGGTGGGAGGTATGTGGTCTTATTTCACTTTTTGGAGCTTGTTACTATTTGAGATCCCATGTCGATTGGAGAGGGGAACGAAACATTCATTTTAAGAAGGTAGAAACCTCTAGGAGATGTGTTTTAAAATCGTGAGGTTGATGGTGATACGTAACAAGCCAAAGCGGACAATATGTACTAGCGGTGGACTTGGGCTGTTACAAATCGTATTAGAGGCAAACACCGGACAATGTGCCAACAAGGACGCTTGAGACCTCAAGGGAGGTGGATTGTGAGATCCCAGAGAGGGGAACAAAACATTCCTTATAAGGATGTGGAAATTGCTCCCTAGCAAACACGTTATAAAACCGTAAGGCTGACGACGATACGTAACGAGTCAAAGCAGAAAATATCTGCTAGCCAATGGACTTGGACTGTTACAAATGATATTTAGAGCCAGACATTGGACAGTGTGTCAATGAGGACGCTGGACAACCAAGGAGGGTGGATTGTGAGATCCCTCGTTAGTTGAAGAGGGGAACAAAGTATTCTTTATAAGGATATGGAAATCTCTCCCTAATAGACGTGTTTTAAAATCGTTAGACTAACGACGATACATAATGAATCAAAACGGACAATATCTACTGGCGGTGAGCTTAAGCTGGTACATCATAACTTTAAATTTGAAGTTATGGTCGACAGAAATCCGAATTGAATGGAGCATCTAAACATTTCATGGCATTTGGTGGGGGCATGAGGTTATGTGTTGGTACAGATTTTACCAAACTTCAAATGGCTGTGTTTCTCCACTGCCTGGTTACAAAGTACACGTAAGTTGCAAGAATGAGATGCTAACTCTCTCTACACCTAAATCTCGTGTTCGACAATCATTTAGTTCTCGATTCGTAGATTCTTTTTTGGTTCTATTTATAAGTTTTTTGTTTTGTTACGTACCATTTACAAATGTTTTTCAAAATTCAAGACAAGTTTTGAAAACTAATAAATCGAAAGAGAAACTAATGCTCGTACATTTGGGACTGTCAGGTTCAAAGCAATCAAGGGAGGCAATATCGTCCGAACGCCCGGTTTACAGTTTCCCGACGGTTTACACGTTCAAATCATGGAGAAATGACAAAAATAAAAGCCTTGATGAAGAAGATGAACAGAAGTAG

mRNA sequence

GAGCACATCCAAGCACCACTTACCCACAATACTTCCATTTCAGTTTGCAGCAACCAAAATGTGGGTTCTTTTGATAGGAACTATTGTTGTTTTGAGCTTTACACACTGGCTTTACCGTTGGAGAAACCCCAAATGTAATGGGAAACTCCCACCAGGTTCAATGGGGTTCCCTCTGCTTGGTGAAACCTTCCAGTTCTTCGCTCCCAATACCTCCTCTGATGTCCCTCCTTTCATCAGAAACAGAGTGGATAGGTACGGACCAATCTTCAGAACAAGTTTGGTGGGAAGACCGCTGATTGTCTCAACCGACCCCGACTTCAATCACTTCATCTTTCAACAAGAGGGTCACTTGTTCCAGAGCTGGTATCCATCTACCTTCACCCAGATATTTGGAAGGCAAAATGTTGGTTCATTGCACGGTTTCATGTATAAGTTCCTGAAGAACATGGTTCTTCATCTTGTTGGCCCTGAAAATCTGAGGAAGATGATATCTGAAGTGGAGGCTGTAGCTACCACAAGATTGACACAATGGTCATCTCTTGAATCGGTTGAATTGAAGGATGAGACTGCAAGTATGATTTTTAATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTGAAGAGAATTTGAGAGACAACTTCGTTGCATTCATACAGGGCTTGATTTCCTTCCCTCTAAATGTTTTTGGAACAGCTTATAACAAATGTTTACAGGGCAGGAAAAGAGCGATGAGGATGCTGAAAAACATGCTACAAGAAAGGCGTGCAAATCCAAGGAAAGAGCAAACAGATTTTTTTGATTATGTTCTTGAAGAACTGGCGAAGGATGGAACTCTTCTCACCGAGGAGATTGCTTTGGACCTCATGTTTGTGCTGCTTTTTGCGAGCTTCGAAACTACTTCACTTGCTATTACTGCAGCCATTAAATTCCTCCTCAACAATCCTCATGTGCTGGAGGAGCTGACGTGTGAACATGAGGGGATATTGAAGGCAAGGGAGAATCCAGATTGTGGGCTTACATGGGATGAATACAAATCCATGACATTCACATTTCAGTTCATCAATGAAACTGTGAGACTGGCAAATATAGTTCCGGGAATCTTTCGAAAAGCACTGAGAGACATCCAGTTTAAGGGATATACCATTCCAGCTGGGTGGTCAGTTATGGTCTGCCCTCCTGCCGTGCACTTGAATCCTGAAAAATATGTTGATCCCCTCGCTTTTAATCCATGGCGGTGGGAGATTTTACCAAACTTCAAATGGCTGTGTTTCTCCACTGCCTGGTTACAAAGTACACGTTCAAAGCAATCAAGGGAGGCAATATCGTCCGAACGCCCGGTTTACAGTTTCCCGACGGTTTACACGTTCAAATCATGGAGAAATGACAAAAATAAAAGCCTTGATGAAGAAGATGAACAGAAGTAG

Coding sequence (CDS)

ATGTGGGTTCTTTTGATAGGAACTATTGTTGTTTTGAGCTTTACACACTGGCTTTACCGTTGGAGAAACCCCAAATGTAATGGGAAACTCCCACCAGGTTCAATGGGGTTCCCTCTGCTTGGTGAAACCTTCCAGTTCTTCGCTCCCAATACCTCCTCTGATGTCCCTCCTTTCATCAGAAACAGAGTGGATAGGTACGGACCAATCTTCAGAACAAGTTTGGTGGGAAGACCGCTGATTGTCTCAACCGACCCCGACTTCAATCACTTCATCTTTCAACAAGAGGGTCACTTGTTCCAGAGCTGGTATCCATCTACCTTCACCCAGATATTTGGAAGGCAAAATGTTGGTTCATTGCACGGTTTCATGTATAAGTTCCTGAAGAACATGGTTCTTCATCTTGTTGGCCCTGAAAATCTGAGGAAGATGATATCTGAAGTGGAGGCTGTAGCTACCACAAGATTGACACAATGGTCATCTCTTGAATCGGTTGAATTGAAGGATGAGACTGCAAGTATGATTTTTAATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTGAAGAGAATTTGAGAGACAACTTCGTTGCATTCATACAGGGCTTGATTTCCTTCCCTCTAAATGTTTTTGGAACAGCTTATAACAAATGTTTACAGGGCAGGAAAAGAGCGATGAGGATGCTGAAAAACATGCTACAAGAAAGGCGTGCAAATCCAAGGAAAGAGCAAACAGATTTTTTTGATTATGTTCTTGAAGAACTGGCGAAGGATGGAACTCTTCTCACCGAGGAGATTGCTTTGGACCTCATGTTTGTGCTGCTTTTTGCGAGCTTCGAAACTACTTCACTTGCTATTACTGCAGCCATTAAATTCCTCCTCAACAATCCTCATGTGCTGGAGGAGCTGACGTGTGAACATGAGGGGATATTGAAGGCAAGGGAGAATCCAGATTGTGGGCTTACATGGGATGAATACAAATCCATGACATTCACATTTCAGTTCATCAATGAAACTGTGAGACTGGCAAATATAGTTCCGGGAATCTTTCGAAAAGCACTGAGAGACATCCAGTTTAAGGGATATACCATTCCAGCTGGGTGGTCAGTTATGGTCTGCCCTCCTGCCGTGCACTTGAATCCTGAAAAATATGTTGATCCCCTCGCTTTTAATCCATGGCGGTGGGAGATTTTACCAAACTTCAAATGGCTGTGTTTCTCCACTGCCTGGTTACAAAGTACACGTTCAAAGCAATCAAGGGAGGCAATATCGTCCGAACGCCCGGTTTACAGTTTCCCGACGGTTTACACGTTCAAATCATGGAGAAATGACAAAAATAAAAGCCTTGATGAAGAAGATGAACAGAAGTAG

Protein sequence

MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLHGFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAKKLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRANPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQFKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEILPNFKWLCFSTAWLQSTRSKQSREAISSERPVYSFPTVYTFKSWRNDKNKSLDEEDEQK
Homology
BLAST of CmoCh06G007360 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 523.5 bits (1347), Expect = 2.5e-147
Identity = 245/401 (61.10%), Postives = 315/401 (78.55%), Query Frame = 0

Query: 3   VLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNR 62
           +L      V++   W YRW +P+ NG+LPPGS+G P++GET QFFAPN + D+ PF++ R
Sbjct: 44  LLCAALAAVVALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKER 103

Query: 63  VDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLHGF 122
           + RYG IF+TS+VGRP++VS DP+ N+++FQQEG LF+SWYP TFT+IFGR NVGSLHGF
Sbjct: 104 IKRYGSIFKTSVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVGSLHGF 163

Query: 123 MYKFLKNMVLHLVGPENLRK-MISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAKK 182
           MYK+LK +VL L G ENL+  +++E +A     L  W+S  SVELK+  ++MIF+LTAKK
Sbjct: 164 MYKYLKTLVLRLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAKK 223

Query: 183 LISYD-SENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 242
           LI YD S+ S+ NLR NF AFI GLISFPLN+ GTAY++C++GRK AM++L+ M++ER A
Sbjct: 224 LIGYDPSKPSQVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGMMKERMA 283

Query: 243 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 302
            P +   DFFD+V++EL ++  LLTE IALDLMFVLLFASFETT+LA+T  +K L  NP 
Sbjct: 284 EPERPCEDFFDHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVKLLTENPK 343

Query: 303 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 362
           V++ L  EHE I++ R++P+ G+TW EYKSMTFT Q I E VRLANIVPGIFRKAL+D++
Sbjct: 344 VVDALREEHEAIIRNRKDPNSGVTWAEYKSMTFTSQVIMEIVRLANIVPGIFRKALQDVE 403

Query: 363 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEILP 402
            KGYTIPAGW +MVCPPAVHLNPE Y DPLAFNPWRW+  P
Sbjct: 404 IKGYTIPAGWGIMVCPPAVHLNPEIYEDPLAFNPWRWQGKP 444

BLAST of CmoCh06G007360 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 5.4e-94
Identity = 167/404 (41.34%), Postives = 253/404 (62.62%), Query Frame = 0

Query: 1   MWVLLIG--TIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPF 60
           MW +++G  T+ V  + HW+ +WR+ K NG LPPG+MG PL+GET Q   P+ S DV PF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IRNRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVG- 120
           I+ +V+RYGPIF+T L GRP++VS D +FN++I  QEG   + WY  T ++ FG      
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 SLHGFMYKFLKNMVLHLVGPENLR-KMISEVEAVATTRLTQWSSLESVELKDETASMIFN 180
              G ++K+++++ L+  G E LR + +  +EA +   L  WS+  SVE+K+ +A M+F 
Sbjct: 121 KALGLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVFR 180

Query: 181 LTAKKLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQ 240
            +  K+   D++    N+   F   + G +S PLN  GT Y+KCL+  K   + L+ ++ 
Sbjct: 181 TSVNKMFGEDAKKLSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVVD 240

Query: 241 ERRANPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLL 300
           +R AN   +  DF    L++   +   ++EE  + L+F + FASFE+ S  +T  +K L 
Sbjct: 241 DRLANVGPDVEDFLGQALKDKESE-KFISEEFIIQLLFSISFASFESISTTLTLILKLLD 300

Query: 301 NNPHVLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKAL 360
            +P V++EL  EHE I KAR +PD  +TW+EYKSMTFT Q INET+RL ++ P + RK +
Sbjct: 301 EHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLQVINETLRLGSVTPALLRKTV 360

Query: 361 RDIQFKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEIL 401
           +D+Q KGY IP GW++M+   + H +P+ Y DP  FNPWRW+ L
Sbjct: 361 KDLQVKGYIIPEGWTIMLVTASRHRDPKVYKDPHIFNPWRWKDL 403

BLAST of CmoCh06G007360 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 313.2 bits (801), Expect = 5.0e-84
Identity = 164/401 (40.90%), Postives = 243/401 (60.60%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           +WV+ +  +V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   ++ PF++
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120
            R+ +YGP+FRT++ G   +V T+PD    +F+QE   F   YP  F + FG++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMYKFLKNMVLHLVGPENL-RKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTA 180
           G ++K +K + L  +G E L +KMI E++ V    L   ++  S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSEENLRDNFVAFIQGLISFPLNVFG-TAYNKCLQGRKRAMRMLKNMLQER 240
            K+IS     ++  L DN +A        PL +    +  K    R+ A++++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNN 300
           +A+ R+   DF D ++EE  K+  +  EE A++L+F +L  + E+TS   + AIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTCEHEGILKARENPDCGLTWDEYK-SMTFTFQFINETVRLANIVPGIFRKALR 360
              L EL  EH  IL+ R     G++W+EY+  MTFT   INET+R+AN+ P ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIQFKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           D++ KGYTIPAGW V V PPAVH N   Y +PL FNPWRWE
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWE 405

BLAST of CmoCh06G007360 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 271.2 bits (692), Expect = 2.2e-71
Identity = 160/423 (37.83%), Postives = 237/423 (56.03%), Query Frame = 0

Query: 3   VLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNR 62
           +LL+ +++ L     L + RN K    LPPG  G+P LGET  +  P T++ +  F++  
Sbjct: 12  LLLLPSLLSLLLFLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQH 71

Query: 63  VDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLHGF 122
           V +YG I+R++L G P IVS D   N FI Q EG LF+  YP +   I G+ ++  L G 
Sbjct: 72  VSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGD 131

Query: 123 MYKFLKNMVLHLVGPENLRK-MISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAKK 182
           M++ ++++ L+ +    LR  ++ +VE      L  W        +DE     FNL AK 
Sbjct: 132 MHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKH 191

Query: 183 LISYD-SENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 242
           ++S D  E   E L+  +V F++G++S PLN+ GTAY+K LQ R   ++ ++  ++ER+ 
Sbjct: 192 IMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKL 251

Query: 243 ------------------------NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVL 302
                                   + RK++TD  D +L  + K   L TE+I LDL+  L
Sbjct: 252 DIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTD--DDLLGWVLKHSNLSTEQI-LDLILSL 311

Query: 303 LFASFETTSLAITAAIKFLLNNPHVLEELTCEHEGILKA-RENPDCGLTWDEYKSMTFTF 362
           LFA  ET+S+AI  AI FL   P  +EEL  EH  I +A +E  +  L WD+YK M FT 
Sbjct: 312 LFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQ 371

Query: 363 QFINETVRLANIVPGIFRKALRDIQFKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPW 399
             INET+RL N+V  + RKAL+D+++KGY IP+GW V+    AVHL+  +Y  P  FNPW
Sbjct: 372 CVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPW 431

BLAST of CmoCh06G007360 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 4.9e-71
Identity = 147/396 (37.12%), Postives = 220/396 (55.56%), Query Frame = 0

Query: 4   LLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNRV 63
           LL+ + +   F   L R R  +    LPPGS+G PL+GETFQ      + +  PFI  RV
Sbjct: 7   LLLLSSIAAGFLLLLRRTRYRRMG--LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERV 66

Query: 64  DRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLHGFM 123
            RYG +F T L G P I S DP+ N F+ Q EG LF+  YP++   + G+ ++  + G +
Sbjct: 67  ARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSL 126

Query: 124 YKFLKNMVLHLVGPENLR-KMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAKKL 183
           +K + ++ +       ++  ++ +++ +    L  WSS   V L +E   + F LT K+L
Sbjct: 127 HKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVKQL 186

Query: 184 ISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRANP 243
           +S+D     E+LR  ++  I+G  S PL +F T Y K +Q R++    L  ++ +RR   
Sbjct: 187 MSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEE 246

Query: 244 RKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVL 303
            +      D +   LA D     EEI +D +  LL A +ETTS  +T A+KFL   P  L
Sbjct: 247 EEGAERKKDMLAALLAADDGFSDEEI-VDFLVALLVAGYETTSTIMTLAVKFLTETPLAL 306

Query: 304 EELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQFK 363
            +L  EHE I +A ++    L W +YKSM FT   +NET+R+ANI+ G+FR+A+ D++ K
Sbjct: 307 AQLKEEHEKI-RAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIK 366

Query: 364 GYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           GY IP GW V     AVHL+P  + D   FNPWRW+
Sbjct: 367 GYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQ 396

BLAST of CmoCh06G007360 vs. ExPASy TrEMBL
Match: A0A6J1G055 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449479 PE=4 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 2.7e-234
Identity = 399/402 (99.25%), Postives = 400/402 (99.50%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120

Query: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180
           GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360
           VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEILPN 403
           FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE+  N
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEVQSN 402

BLAST of CmoCh06G007360 vs. ExPASy TrEMBL
Match: A0A6J1G031 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449479 PE=3 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.8e-233
Identity = 398/398 (100.00%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120

Query: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180
           GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360
           VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 398

BLAST of CmoCh06G007360 vs. ExPASy TrEMBL
Match: A0A6J1FUR3 (cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111448609 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.6e-226
Identity = 385/398 (96.73%), Postives = 392/398 (98.49%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFT+I GR+NVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120

Query: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180
           GFM+KF+KNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKEQTDFFD+VLEELAKDGTLLTEEIALDL+FVLLFASFETTSLAIT AIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLIFVLLFASFETTSLAITLAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360
           VLEELTCEHEGILKARENPDCGLTW EYKSMTFTFQFINETVRLANI PGIFRKALRDI 
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           FKGYTIPAGWSVMVCPPAVHLNPE YVDPLAFNPWRW+
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 398

BLAST of CmoCh06G007360 vs. ExPASy TrEMBL
Match: A0A6J1KXA8 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498396 PE=4 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.7e-224
Identity = 385/417 (92.33%), Postives = 395/417 (94.72%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLL+G IVVLSFTH LYRWRNPKCNGKLPPGSMGFPLLGET QFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLMGAIVVLSFTHCLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEG+LFQSWYPSTFT+IFGRQNVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGYLFQSWYPSTFTEIFGRQNVGSLH 120

Query: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180
           GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRL QWS LESVELKDETASMIF+LTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLRQWSCLESVELKDETASMIFDLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENS ENLR+NFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSGENLRENFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKE  DFFD+VLEELAKDGTLLTEEIALDLMFVLLF+SFETTS+AITAAIKFLLNNPH
Sbjct: 241 NPRKEHMDFFDFVLEELAKDGTLLTEEIALDLMFVLLFSSFETTSIAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360
           VLEELTCEHEGILKARENPDCGLTW EYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEILPNFKWLCFSTAWLQSTR 418
           FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE+  N        AW   +R
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEVQSNQGRQYRPNAWFTVSR 417

BLAST of CmoCh06G007360 vs. ExPASy TrEMBL
Match: A0A6J1KZ20 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498396 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.9e-223
Identity = 381/398 (95.73%), Postives = 389/398 (97.74%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLL+G IVVLSFTH LYRWRNPKCNGKLPPGSMGFPLLGET QFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLMGAIVVLSFTHCLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEG+LFQSWYPSTFT+IFGRQNVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGYLFQSWYPSTFTEIFGRQNVGSLH 120

Query: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180
           GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRL QWS LESVELKDETASMIF+LTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLRQWSCLESVELKDETASMIFDLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENS ENLR+NFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSGENLRENFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKE  DFFD+VLEELAKDGTLLTEEIALDLMFVLLF+SFETTS+AITAAIKFLLNNPH
Sbjct: 241 NPRKEHMDFFDFVLEELAKDGTLLTEEIALDLMFVLLFSSFETTSIAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360
           VLEELTCEHEGILKARENPDCGLTW EYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 398

BLAST of CmoCh06G007360 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 610.9 bits (1574), Expect = 8.3e-175
Identity = 291/399 (72.93%), Postives = 341/399 (85.46%), Query Frame = 0

Query: 1   MWVLLIG-TIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFI 60
           MW LLI  +++++S THW+Y WRNPKC GKLPPGSMGFPLLGE+ QFF PN +SD+PPFI
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  RNRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSL 120
           + RV +YGPIF+T+LVGRP+IVSTD D ++F+F QEG  FQSWYP TFT IFG++NVGSL
Sbjct: 61  KERVKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSL 120

Query: 121 HGFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTA 180
           HGFMYK+LKNMVL L G + L+KM+ +VE  A  RL  WS+ +SVELKD TASMIF+LTA
Sbjct: 121 HGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASMIFDLTA 180

Query: 181 KKLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERR 240
           KKLIS+D + S ENLR NFVAFIQGLISFP ++ GTAY+KCLQGR +AM+ML+NMLQERR
Sbjct: 181 KKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR 240

Query: 241 ANPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNP 300
            NPRK  +DFFDYV+EE+ K+GT+LTEEIALDLMFVLLFASFETTSLA+T AIKFL ++P
Sbjct: 241 ENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDP 300

Query: 301 HVLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDI 360
            VL+ LT EHE IL+ RE+ D GLTW+EYKSMT+TFQFINET RLANIVP IFRKALRDI
Sbjct: 301 EVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDI 360

Query: 361 QFKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           +FK YTIPAGW+VMVCPPAVHLNPE Y DPL FNP RWE
Sbjct: 361 KFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWE 399

BLAST of CmoCh06G007360 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 605.5 bits (1560), Expect = 3.5e-173
Identity = 292/405 (72.10%), Postives = 341/405 (84.20%), Query Frame = 0

Query: 1   MWVLLIG-TIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFI 60
           MW LLI  +++++S THW+Y WRNPKC GKLPPGSMGFPLLGE+ QFF PN +SD+PPFI
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  RNRVD------RYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGR 120
           + RV       RYGPIF+T+LVGRP+IVSTD D ++F+F QEG  FQSWYP TFT IFG+
Sbjct: 61  KERVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGK 120

Query: 121 QNVGSLHGFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASM 180
           +NVGSLHGFMYK+LKNMVL L G + L+KM+ +VE  A  RL  WS+ +SVELKD TASM
Sbjct: 121 KNVGSLHGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASM 180

Query: 181 IFNLTAKKLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKN 240
           IF+LTAKKLIS+D + S ENLR NFVAFIQGLISFP ++ GTAY+KCLQGR +AM+ML+N
Sbjct: 181 IFDLTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRN 240

Query: 241 MLQERRANPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIK 300
           MLQERR NPRK  +DFFDYV+EE+ K+GT+LTEEIALDLMFVLLFASFETTSLA+T AIK
Sbjct: 241 MLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIK 300

Query: 301 FLLNNPHVLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFR 360
           FL ++P VL+ LT EHE IL+ RE+ D GLTW+EYKSMT+TFQFINET RLANIVP IFR
Sbjct: 301 FLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFR 360

Query: 361 KALRDIQFKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           KALRDI+FK YTIPAGW+VMVCPPAVHLNPE Y DPL FNP RWE
Sbjct: 361 KALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWE 405

BLAST of CmoCh06G007360 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 328.6 bits (841), Expect = 8.2e-90
Identity = 165/398 (41.46%), Postives = 249/398 (62.56%), Query Frame = 0

Query: 4   LLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNRV 63
           +L+  +VV+  +HWLYRW NPKC GKLPPGSMGFP++GET  FF P     +P F++ R+
Sbjct: 9   MLMVALVVVRISHWLYRWSNPKCPGKLPPGSMGFPIIGETLDFFKPCGVEGIPTFVKKRM 68

Query: 64  DRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLHGFM 123
            RYGP+FRT++ G   +VSTDPD  H IF+QE   F+  YP  F ++FG+ N+     F+
Sbjct: 69  IRYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFLKEVFI 128

Query: 124 YKFLKNMVLHLVGPENLRK-MISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAKKL 183
           +K+L+ + + ++G E L++ M+  ++      +   +S  S  ++ E  +++      KL
Sbjct: 129 HKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVAYMTPKL 188

Query: 184 ISYDSENSEENLRDNFVAF-IQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRAN 243
           IS     ++  L DN  AF +    SF       A  K L+ R+ A++++K++L  R+  
Sbjct: 189 ISNLKPETQSKLIDNLNAFNLDWFKSFLRLSTWKAVTKALKSREEAIQVMKDVLMMRK-E 248

Query: 244 PRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHV 303
            R++Q DF + +LEEL KDG+   +  A++L+F+L FA  E TS     A+KF+  +P V
Sbjct: 249 TREKQEDFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALAVKFISKDPKV 308

Query: 304 LEELTCEHEGILKARENPDCGLTWDEYK-SMTFTFQFINETVRLANIVPGIFRKALRDIQ 363
           L EL  EH+ I+  R++ + G++W+EY+ +MTFT    NE +RLAN  P +FRKA++D++
Sbjct: 309 LAELKREHKAIVDNRKDKEAGVSWEEYRHNMTFTNMVSNEVLRLANTTPLLFRKAVQDVE 368

Query: 364 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
            KGYTIPAGW V V P AVH +P  Y +P  FNPWRWE
Sbjct: 369 IKGYTIPAGWIVAVAPSAVHFDPAIYENPFEFNPWRWE 405

BLAST of CmoCh06G007360 vs. TAIR 10
Match: AT5G48000.2 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 313.2 bits (801), Expect = 3.6e-85
Identity = 164/401 (40.90%), Postives = 243/401 (60.60%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           +WV+ +  +V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   ++ PF++
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120
            R+ +YGP+FRT++ G   +V T+PD    +F+QE   F   YP  F + FG++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMYKFLKNMVLHLVGPENL-RKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTA 180
           G ++K +K + L  +G E L +KMI E++ V    L   ++  S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSEENLRDNFVAFIQGLISFPLNVFG-TAYNKCLQGRKRAMRMLKNMLQER 240
            K+IS     ++  L DN +A        PL +    +  K    R+ A++++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNN 300
           +A+ R+   DF D ++EE  K+  +  EE A++L+F +L  + E+TS   + AIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTCEHEGILKARENPDCGLTWDEYK-SMTFTFQFINETVRLANIVPGIFRKALR 360
              L EL  EH  IL+ R     G++W+EY+  MTFT   INET+R+AN+ P ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIQFKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           D++ KGYTIPAGW V V PPAVH N   Y +PL FNPWRWE
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWE 405

BLAST of CmoCh06G007360 vs. TAIR 10
Match: AT5G48000.3 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 313.2 bits (801), Expect = 3.6e-85
Identity = 164/401 (40.90%), Postives = 243/401 (60.60%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           +WV+ +  +V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   ++ PF++
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120
            R+ +YGP+FRT++ G   +V T+PD    +F+QE   F   YP  F + FG++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMYKFLKNMVLHLVGPENL-RKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTA 180
           G ++K +K + L  +G E L +KMI E++ V    L   ++  S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSEENLRDNFVAFIQGLISFPLNVFG-TAYNKCLQGRKRAMRMLKNMLQER 240
            K+IS     ++  L DN +A        PL +    +  K    R+ A++++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNN 300
           +A+ R+   DF D ++EE  K+  +  EE A++L+F +L  + E+TS   + AIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTCEHEGILKARENPDCGLTWDEYK-SMTFTFQFINETVRLANIVPGIFRKALR 360
              L EL  EH  IL+ R     G++W+EY+  MTFT   INET+R+AN+ P ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIQFKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 399
           D++ KGYTIPAGW V V PPAVH N   Y +PL FNPWRWE
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWE 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XU382.5e-14761.10Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV... [more]
K7NBR25.4e-9441.34Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=... [more]
Q8L7D55.0e-8440.90Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3[more]
O649892.2e-7137.83Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
Q425694.9e-7137.12Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G0552.7e-23499.25cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114494... [more]
A0A6J1G0311.8e-233100.00cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114494... [more]
A0A6J1FUR33.6e-22696.73cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111448609 PE=3 SV=... [more]
A0A6J1KXA85.7e-22492.33cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498396... [more]
A0A6J1KZ204.9e-22395.73cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498396... [more]
Match NameE-valueIdentityDescription
AT1G12740.18.3e-17572.93cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT1G12740.23.5e-17372.10cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT1G78490.18.2e-9041.46cytochrome P450, family 708, subfamily A, polypeptide 3 [more]
AT5G48000.23.6e-8540.90cytochrome P450, family 708, subfamily A, polypeptide 2 [more]
AT5G48000.33.6e-8540.90cytochrome P450, family 708, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 380..398
score: 37.25
coord: 364..378
score: 29.23
coord: 331..347
score: 27.15
coord: 57..80
score: 26.76
IPR001128Cytochrome P450PFAMPF00067p450coord: 31..398
e-value: 8.8E-47
score: 159.9
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 22..410
e-value: 1.2E-90
score: 306.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..398
NoneNo IPR availablePANTHERPTHR24286:SF11CYTOCHROME P450, FAMILY 87, SUBFAMILY A, POLYPEPTIDE 2coord: 8..403
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 8..403

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G007360.1CmoCh06G007360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen