CmoCh06G006940 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh06G006940
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	caffeic acid 3-O-methyltransferase-like
Location	Cmo_Chr06: 3529402 .. 3529759 (+)
RNA-Seq Expression	CmoCh06G006940
Synteny	CmoCh06G006940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGAGTGGTTCCGACGGTGCCGGAGACGACAGCTGCAGAGAAAGCCATCAGCCAAGGCGATTTGCTTATGATGACTCAAAACCCGGGAGGAAAGGAGCGAAGTCGCGATGAGTTTAAAGCCCTCGCAACCAAAGCTGGGTTTAAACACATCAAATTGGTCTGTTTCCTATGCAATTTTTGGATCATAGAGTTCTTGAAATGAAGTTATATGTCTACTTCTTCACGCTTGTATTGCCTTTTAAGTGCTTGTCTATTGGTACCGTTTTCACTGTATTGTTTAGAATGTCGGGAAAAACAATACTCGAGAATCAAAGCATTTTCAAATTAAATTTTCTTTATGTTACCCGTATTAA

mRNA sequence

ATGGAGGGAGTGGTTCCGACGGTGCCGGAGACGACAGCTGCAGAGAAAGCCATCAGCCAAGGCGATTTGCTTATGATGACTCAAAACCCGGGAGGAAAGGAGCGAAGTCGCGATGAGTTTAAAGCCCTCGCAACCAAAGCTGGGTTTAAACACATCAAATTGAATGTCGGGAAAAACAATACTCGAGAATCAAAGCATTTTCAAATTAAATTTTCTTTATGTTACCCGTATTAA

Coding sequence (CDS)

ATGGAGGGAGTGGTTCCGACGGTGCCGGAGACGACAGCTGCAGAGAAAGCCATCAGCCAAGGCGATTTGCTTATGATGACTCAAAACCCGGGAGGAAAGGAGCGAAGTCGCGATGAGTTTAAAGCCCTCGCAACCAAAGCTGGGTTTAAACACATCAAATTGAATGTCGGGAAAAACAATACTCGAGAATCAAAGCATTTTCAAATTAAATTTTCTTTATGTTACCCGTATTAA

Protein sequence

MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKLNVGKNNTRESKHFQIKFSLCYPY

Homology

[Close]

BLAST of CmoCh06G006940 vs. ExPASy Swiss-Prot
Match: Q8GU25 (Caffeic acid 3-O-methyltransferase OS=Rosa chinensis OX=74649 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 9.2e-07
Identity = 25/60 (41.67%), Postives = 39/60 (65.00%), Query Frame = 0


Query: 2   EGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKLNVGKNNT 61
           E ++P  P+T+ A K +   D++M+  NPGGKER+  EF+ALA  +GF+ I++     NT
Sbjct: 297 ECILPVAPDTSLATKGVVHIDVVMLAHNPGGKERTEQEFEALAKGSGFQGIRVACNAFNT 356

[Close]

BLAST of CmoCh06G006940 vs. ExPASy Swiss-Prot
Match: P28002 (Caffeic acid 3-O-methyltransferase OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.7e-06
Identity = 24/60 (40.00%), Postives = 39/60 (65.00%), Query Frame = 0


Query: 2   EGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKLNVGKNNT 61
           E ++P  P+++ A K +   D++M+  NPGGKER++ EF+ LA  AGF+  K++    NT
Sbjct: 297 ECILPVAPDSSLATKGVVHIDVIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNT 356

[Close]

BLAST of CmoCh06G006940 vs. ExPASy Swiss-Prot
Match: Q41086 (Caffeic acid 3-O-methyltransferase 2 OS=Populus tremuloides OX=3693 GN=OMT2 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.7e-06
Identity = 25/61 (40.98%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 1   MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKLNVGKNN 60
           +E ++P  P+T+ A K +   D++M+  NPGGKER+  EF++LA  AGFK  ++     N
Sbjct: 295 VECILPVAPDTSLATKGVMHVDVIMLAHNPGGKERTDREFESLARGAGFKGFEVMCCAFN 354

Query: 61  T 62
           T
Sbjct: 355 T 355

[Close]

BLAST of CmoCh06G006940 vs. ExPASy Swiss-Prot
Match: Q8LL87 (Caffeic acid 3-O-methyltransferase OS=Coffea canephora OX=49390 GN=COMT PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 0


Query: 2   EGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKLNVGKNNT 61
           E V+P  P+T+ A + +   D++M+  NPGGKER+  EF+ALA  AGFK  +      NT
Sbjct: 284 ECVLPEAPDTSLATQNVVHVDVVMLAHNPGGKERTEKEFEALAKGAGFKEFRKVCSAVNT 343

[Close]

BLAST of CmoCh06G006940 vs. ExPASy Swiss-Prot
Match: Q43239 (Caffeic acid 3-O-methyltransferase OS=Zinnia violacea OX=34245 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 24/49 (48.98%), Postives = 34/49 (69.39%), Query Frame = 0


Query: 2   EGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFK 51
           E ++P  P+TT A + +   D++M+  NPGGKER+  EF+ALA  AGFK
Sbjct: 288 ECILPEAPDTTPATQNVIHIDVIMLAHNPGGKERTEKEFEALAKGAGFK 336

[Close]

BLAST of CmoCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1FG58 (caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443875 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.0e-20
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 0


Query: 1   MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKL 55
           MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKL
Sbjct: 302 MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKL 355

[Close]

BLAST of CmoCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1FBC5 (caffeic acid 3-O-methyltransferase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111443875 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.0e-20
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 0


Query: 1   MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKL 55
           MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKL
Sbjct: 302 MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKL 355

[Close]

BLAST of CmoCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1FB75 (caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443843 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 5.8e-20
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 1   MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIK 54
           MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIK
Sbjct: 302 MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIK 354

[Close]

BLAST of CmoCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1FAC2 (caffeic acid 3-O-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443843 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 5.8e-20
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 1   MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIK 54
           MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIK
Sbjct: 303 MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIK 355

[Close]

BLAST of CmoCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1FGP5 (caffeic acid 3-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111443858 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.5e-15
Identity = 43/53 (81.13%), Postives = 49/53 (92.45%), Query Frame = 0


Query: 1   MEGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIK 54
           MEGV+PTVPET+ A K I QGD+LMMTQNPGGKER+RDEF+ALATKAGFKH+K
Sbjct: 302 MEGVLPTVPETSDAAKGIIQGDVLMMTQNPGGKERTRDEFEALATKAGFKHVK 354

[Close]

BLAST of CmoCh06G006940 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 51.6 bits (122), Expect = 3.3e-07
Identity = 23/53 (43.40%), Postives = 36/53 (67.92%), Query Frame = 0


Query: 2   EGVVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKL 55
           E ++P  P+++ + K +   D +M+  NPGGKER+  EF+ALA  +GFK IK+
Sbjct: 295 ECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKV 347

[Close]

BLAST of CmoCh06G006940 vs. TAIR 10
Match: AT1G33030.1 (O-methyltransferase family protein )

HSP 1 Score: 47.8 bits (112), Expect = 4.7e-06
Identity = 21/51 (41.18%), Postives = 34/51 (66.67%), Query Frame = 0


Query: 4   VVPTVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKL 55
           V+P  P  T  ++++ Q +L MM  NP GKER++ EF+ LA  AGF ++++
Sbjct: 286 VIPEFPGDTLLDRSLFQFELFMMNMNPSGKERTKKEFEILARLAGFSNVQV 336

[Close]

BLAST of CmoCh06G006940 vs. TAIR 10
Match: AT1G21100.1 (O-methyltransferase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 6.8e-05
Identity = 21/32 (65.62%), Postives = 24/32 (75.00%), Query Frame = 0


Query: 22  DLLMMTQNPGGKERSRDEFKALATKAGFKHIK 54
           D+LM TQ  GGKERSR EF+ALA  +GF H K
Sbjct: 327 DMLMFTQCSGGKERSRAEFEALAAASGFTHCK 358

[Close]

BLAST of CmoCh06G006940 vs. TAIR 10
Match: AT1G21130.1 (O-methyltransferase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 6.8e-05
Identity = 21/32 (65.62%), Postives = 24/32 (75.00%), Query Frame = 0


Query: 22  DLLMMTQNPGGKERSRDEFKALATKAGFKHIK 54
           D+LM TQ  GGKERSR EF+ALA  +GF H K
Sbjct: 327 DMLMFTQCSGGKERSRAEFEALAAASGFTHCK 358

[Close]

BLAST of CmoCh06G006940 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 43.5 bits (101), Expect = 8.9e-05
Identity = 27/58 (46.55%), Postives = 35/58 (60.34%), Query Frame = 0


Query: 1   MEGVVP-TVPETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKLNVG 58
           +E +VP  V ET  A K     DL MM+   GGKER++ EF+ LA +AGFK  K+  G
Sbjct: 294 IEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKIIYG 351

The following BLAST results are available for this feature:

BLAST of CmoCh06G006940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GU25	9.2e-07	41.67	Caffeic acid 3-O-methyltransferase OS=Rosa chinensis OX=74649 GN=COMT1 PE=2 SV=1	[more]
P28002	2.7e-06	40.00	Caffeic acid 3-O-methyltransferase OS=Medicago sativa OX=3879 PE=1 SV=1	[more]
Q41086	2.7e-06	40.98	Caffeic acid 3-O-methyltransferase 2 OS=Populus tremuloides OX=3693 GN=OMT2 PE=3...	[more]
Q8LL87	3.5e-06	43.33	Caffeic acid 3-O-methyltransferase OS=Coffea canephora OX=49390 GN=COMT PE=2 SV=...	[more]
Q43239	3.5e-06	48.98	Caffeic acid 3-O-methyltransferase OS=Zinnia violacea OX=34245 PE=2 SV=1	[more]

BLAST of CmoCh06G006940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FG58	2.0e-20	100.00	caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1FBC5	2.0e-20	100.00	caffeic acid 3-O-methyltransferase-like isoform X3 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1FB75	5.8e-20	100.00	caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1FAC2	5.8e-20	100.00	caffeic acid 3-O-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1FGP5	2.5e-15	81.13	caffeic acid 3-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of CmoCh06G006940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54160.1	3.3e-07	43.40	O-methyltransferase 1	[more]
AT1G33030.1	4.7e-06	41.18	O-methyltransferase family protein	[more]
AT1G21100.1	6.8e-05	65.63	O-methyltransferase family protein	[more]
AT1G21130.1	6.8e-05	65.63	O-methyltransferase family protein	[more]
AT1G51990.1	8.9e-05	46.55	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..54 e-value: 7.5E-13 score: 50.5
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 4..50 e-value: 1.9E-5 score: 24.1
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 3..54

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G006940.1	CmoCh06G006940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity