CmoCh06G002330 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G002330
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRibosomal RNA large subunit methyltransferase E
LocationCmo_Chr06: 1244008 .. 1247064 (+)
RNA-Seq ExpressionCmoCh06G002330
SyntenyCmoCh06G002330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTATTTCAATTTTGACCATGAATGTGGAAACCTCTTGTTGTCATTGGAGGAATTTGAAGCTCCAATTGGGTTGTTGCTGTAAGGCTTGGTGACCTGCTGTAACTTTCTTGATCCTTCAGTCATGGGCAAAGCTTCAAGAGATAAGAGGGTCTTGACTACAATTCAATTCGGCTATTTGTCCTTTTGGTTCATTACCTTTTTCCACGTTTTATTATTTACTTCGTCTCGTTTGCAGAATATATACTATAGTAAAGCAAAAGAAGAAGGCTGGCGTGCTCGAAGTGCGTTCAAACTTCTGAACATAGATGAAGAATTTAACATATTTGAAGGTTTGTTCTTGTATTGGGTTCCTCTTTCTTAAAATATTTTTACCTTTCTGCCAGGATGTCAATCACTGAGATGGTCCAGATGAATGGTAATTGGTACTAAATATTCCTTTCCAAGAATACTAATTTCCTTTGTTTTTTAGGAGTTCTATGTCCTGGAGATGTCATTATTCCCTCGTGAACAATGCCGCAATATTTTGGATTAACCTTCCATAACAGAATAGTTTTCCAGAATCCTCTAGAAATCTGTGTGATCTATTTAGTTTTGCAATCTACCTGTGTTATCTTGTTCATGAACTTTCAATTGCAGGAGTCAAGCGAGTGGTGAATTTATGCGCAGCCCCTGGTAGCTGGAGCTAGGTAATCTCTTTTGGGTGTCTTCTTGTTGCAATCTGTTTATCAAACTAGTGGACAATGCTTTATGGGGGAAATACAATTTTTCATATAAAATACAACTAATTCTTACAAGAATTCTGCAATATGAATCTCTCTTCTCATCTAGTTTCTTTTTTTCTTAGTCAGTCAAGTTGAAAGGTTTGCTGTGATCTCCTCTGTTCCTTGAGATTATTGCTTTGACGTGTCGATTATTTTTTACATGTTTAGCATTGGTAAAACTTGTCAAGTATGATAACGAATTACGCGAGGACCATAATCAAAGCTAGGTTTCTGTTGTTTTACATTTTCGAGTTTTTTTTTTTTTTTTTTGTTGCAGCTGAAGTTGCTTTTCCCGTAGTGACTTTTGCAAAACCGAAAGGCAGTCGCAATCCCAGTATAGGTAACCTTTTGGCTTCACTTTTTTTTATTGGTAACTCTATAGGTCTATCTTTCGGTTATAATTGAACTTATTTCCTGAAAATGATACCCTTCTAAGTGAAAATTGTCATTATAGCGTCTGGTTCATAGGATTCTTACGCATGGATTTCAGATCCCATTGAACTTTAGATTGTTATCGTTTAATACTAGGTGGGACTCGTGTCCACCCAACACCAAATTGTGGGGTTTGCTTATGTTCGTGGTGGCATCTGTAATATGAAAAAAAAAACATGTAAAAGAGGACAAGATAGTCAAAAGGCAGCTCACATATTTTCTAAAACTTCGATGGTTATGTTTATGAACATTAAAAGGTTTTGAATTACAACCGTGAATTGTTCTATTTGATTCTAGGTAGTCGGTCGTTTAATACCATTGCTCAAATGTTGAGGTGTCAGGCTGATTAGACTATAACTAGTTACAACTTACAAGTGATTATTTGGCATCATTTAACTTTTAAACAAAGTAGTGACTATGATTTAACTTTATGTTCATGTTGAAGCTCATACGAGGACTGGGTGTGAATGTTGAGGCATTTGTAGTTTGTGAGAATTATTCGCCTCCTGACGGTTTCAACCCATAAGATCCAACCCCTGCTAGAAAAGGTGGGAAGCCCATCAGGAGGAGATGACTTAGGTAAACTCGATGTACGACCTTGATTGGGAGTTCACATATCGAAGGACCTGATACAACATGTTCTTGATCTTTAAAGATTAATATGTTTTACAGCTGGCTATGACCATGTCTTCTTGTCTTTAAACTTTATATAATTAGTGTCTCTGATTTCAATGTGATCAATTCCCTGGTATTTTTGGTTACACAAGAGGAAGTAATAGCATTGCTATTTTGCTGCATTGTGTCCCTCTCAGATTAAAGTATACATCCCAATAAAGTATCCCCTCAGCGGATATGATTCCGATCGTTCATACCCACTGCCCAGAGGTGCCGAGAGAACTTTGATCCTGTGCGACCTCCTATTGCCCCTCCTTACAACAGGGCTCTTGAAATGAAGAAAACCTTCCAGTGAAGGAATTCGAGAACTAAACCTTCCTTGGATTCCGGATTGTGCTATTCAATGTTGGATAACCTGCAAAGTTGATTTGGATGGACGGGTAATTATTAAGAAACAGCAATCATTTCTCCCTCCCCCCTCTCAGTCTCTGTCCTATTTTTGTAAAAAAAAAAAAAAAAAAAAAAATGCTGTGGATCTTGATTTTGATTATTTTAATCGAAATACATAAAGATTAAGAAGATGTATTATGATAAGCACGAGATTGTCCCGCACGAGTCTTACTCATCCTTCATAATTTCCATGGACATGAACCCCACCCCCATGCATACCACAAACAAAAACAAGAAAGGAAAATCATTTTGAGCATTACACATTTTTTAAGGGCATTCTACTGCATGTCGGTCTGCATGTTTCTATGTTTTTCATATGCAAATGTTTGGACAAACTTGGGTTTTACAGGTGGCCCCCCTGATTGTGAATCAACCAACCATCCTTGCTAATGGGAATCTGAGCTATTCAAAGGCGTGGCCCTCCAAATTCACAGGTCTAACTCATCCCTTTCTAACTGTTGCCTTCTAAAAATTTATGACCCGACATATCACGCATAGAGATTCTTGTTGTCTATCCCATTCCCTCCTTTTGGAGATGCCATAATTTAGGCGTTTAATGGTGGACATCATTGAACAAAAATAACTCAGTGAAATAAACTTATATGTTCTTTTTTTTTTTACCTCATCGAGTGATATATTGTAGATATCCAATTTATGTAATTTTATAATGAACAGATATCTGCTCGAGTTCAAGGCATTTAGACAAGGAAAGTTTCGAGCTTCGAAGATTGAAACTCGATTATCATCTGCTACAGCATCATCTTACAGAGACGGGCACGCAAAAGAATGTCTAA

mRNA sequence

ATGCTCTATTTCAATTTTGACCATGAATGTGGAAACCTCTTGTTGTCATTGGAGGAATTTGAAGCTCCAATTGGGTTGTTGCTGTAAGGCTTGGTGACCTGCTGTAACTTTCTTGATCCTTCAGTCATGGGCAAAGCTTCAAGAGATAAGAGGAATATATACTATAGTAAAGCAAAAGAAGAAGGCTGGCGTGCTCGAAGTGCGTTCAAACTTCTGAACATAGATGAAGAATTTAACATATTTGAAGGAGTCAAGCGAGTGGTGAATTTATGCGCAGCCCCTGCTGAAGTTGCTTTTCCCGTAGTGACTTTTGCAAAACCGAAAGGCAGTCGCAATCCCAGTATAGCTCATACGAGGACTGGTGAAGGAATTCGAGAACTAAACCTTCCTTGGATTCCGGATTGTGCTATTCAATGTTGGATAACCTGCAAAGTTGATTTGGATGGACGGGTGGCCCCCCTGATTGTGAATCAACCAACCATCCTTGCTAATGGGAATCTGAGCTATTCAAAGGCGTGGCCCTCCAAATTCACAGATATCTGCTCGAGTTCAAGGCATTTAGACAAGGAAAGTTTCGAGCTTCGAAGATTGAAACTCGATTATCATCTGCTACAGCATCATCTTACAGAGACGGGCACGCAAAAGAATGTCTAA

Coding sequence (CDS)

ATGGGCAAAGCTTCAAGAGATAAGAGGAATATATACTATAGTAAAGCAAAAGAAGAAGGCTGGCGTGCTCGAAGTGCGTTCAAACTTCTGAACATAGATGAAGAATTTAACATATTTGAAGGAGTCAAGCGAGTGGTGAATTTATGCGCAGCCCCTGCTGAAGTTGCTTTTCCCGTAGTGACTTTTGCAAAACCGAAAGGCAGTCGCAATCCCAGTATAGCTCATACGAGGACTGGTGAAGGAATTCGAGAACTAAACCTTCCTTGGATTCCGGATTGTGCTATTCAATGTTGGATAACCTGCAAAGTTGATTTGGATGGACGGGTGGCCCCCCTGATTGTGAATCAACCAACCATCCTTGCTAATGGGAATCTGAGCTATTCAAAGGCGTGGCCCTCCAAATTCACAGATATCTGCTCGAGTTCAAGGCATTTAGACAAGGAAAGTTTCGAGCTTCGAAGATTGAAACTCGATTATCATCTGCTACAGCATCATCTTACAGAGACGGGCACGCAAAAGAATGTCTAA

Protein sequence

MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAPAEVAFPVVTFAKPKGSRNPSIAHTRTGEGIRELNLPWIPDCAIQCWITCKVDLDGRVAPLIVNQPTILANGNLSYSKAWPSKFTDICSSSRHLDKESFELRRLKLDYHLLQHHLTETGTQKNV
Homology
[Close]
BLAST of CmoCh06G002330 vs. ExPASy Swiss-Prot
Match: Q54VA8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjA PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.4e-18
Identity = 43/52 (82.69%), Postives = 49/52 (94.23%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGK+S+DKR+IYY KAKEEGWRARSAFKL+ IDEE+ IFEGVKRVV+LCAAP
Sbjct: 1  MGKSSKDKRDIYYRKAKEEGWRARSAFKLIQIDEEYQIFEGVKRVVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.1e-15
Identity = 37/52 (71.15%), Postives = 47/52 (90.38%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MG++S+DKR+ YY  AKE+GWRARSAFKLL ++E+FN+FEG KRVV+LCAAP
Sbjct: 1  MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. ExPASy Swiss-Prot
Match: Q9VEP1 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase 1 OS=Drosophila melanogaster OX=7227 GN=CG5220 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 9.4e-15
Identity = 36/52 (69.23%), Postives = 45/52 (86.54%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGK S+DKR+IYY +AK+EGWRARSAFKLL++DE + I  GV+R V+LCAAP
Sbjct: 1  MGKTSKDKRDIYYRQAKDEGWRARSAFKLLHVDEAYGILNGVQRAVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 1.6e-14
Identity = 35/52 (67.31%), Postives = 44/52 (84.62%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MG+ S+DKR++YY  AKE GWRARSAFKLL +D+EF +F+GV R V+LCAAP
Sbjct: 1  MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 80.1 bits (196), Expect = 2.7e-14
Identity = 37/52 (71.15%), Postives = 43/52 (82.69%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGK SRDKR+IYY  AKE  WRARSAFKL+ ID+EF I +GV+R V+LCAAP
Sbjct: 1  MGKTSRDKRDIYYRLAKENKWRARSAFKLMQIDDEFQILKGVRRAVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. ExPASy TrEMBL
Match: A0A6J1GES5 (putative tRNA (Cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111453504 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 3.5e-20
Identity = 52/52 (100.00%), Postives = 52/52 (100.00%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP
Sbjct: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. ExPASy TrEMBL
Match: A0A067EPX4 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Citrus sinensis OX=2711 GN=CISIN_1g021155mg PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.5e-18
Identity = 49/59 (83.05%), Postives = 53/59 (89.83%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAPAEVAFPV 60
          MGKASRDKR+IYY KAKEEGWRARSAFKLL IDEEFNIFEGVKRVV+LCAAP   + P+
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQPM 59

[Close]
BLAST of CmoCh06G002330 vs. ExPASy TrEMBL
Match: A0A1U7WEB4 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Nicotiana sylvestris OX=4096 GN=LOC104222937 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 9.4e-18
Identity = 48/52 (92.31%), Postives = 50/52 (96.15%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGKASRDKR+IYY KAKEEGWRARSAFKLL IDEEFNIFEGVKRVV+LCAAP
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. ExPASy TrEMBL
Match: A0A6I9T3P2 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Sesamum indicum OX=4182 GN=LOC105161552 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 9.4e-18
Identity = 48/52 (92.31%), Postives = 50/52 (96.15%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGKASRDKR+IYY KAKEEGWRARSAFKLL IDEEFNIFEGVKRVV+LCAAP
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. ExPASy TrEMBL
Match: A0A2P5YEP2 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Gossypium barbadense OX=3634 GN=ES319_A09G173400v1 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 9.4e-18
Identity = 48/52 (92.31%), Postives = 50/52 (96.15%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGKASRDKR+IYY KAKEEGWRARSAFKLL IDEEFNIFEGVKRVV+LCAAP
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 1.8e-21
Identity = 48/52 (92.31%), Postives = 50/52 (96.15%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGKASRDKR+IYY KAKEEGWRARSAFKLL IDEEFNIFEGVKRVV+LCAAP
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 1.8e-21
Identity = 48/52 (92.31%), Postives = 50/52 (96.15%), Query Frame = 0 

Query: 1  MGKASRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGKASRDKR+IYY KAKEEGWRARSAFKLL IDEEFNIFEGVKRVV+LCAAP
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAP 52

[Close]
BLAST of CmoCh06G002330 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 46.6 bits (109), Expect = 2.4e-05
Identity = 21/53 (39.62%), Postives = 36/53 (67.92%), Query Frame = 0 

Query: 1  MGKA-SRDKRNIYYSKAKEEGWRARSAFKLLNIDEEFNIFEGVKRVVNLCAAP 53
          MGK   + + + YY  AKE G+R+R+++KLL +D ++++      V++LCAAP
Sbjct: 1  MGKVKGKHRLDKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAP 53

The following BLAST results are available for this feature:
BLAST of CmoCh06G002330 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q54VA82.4e-1882.69Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyosteli... [more]
O360151.1e-1571.15Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch... [more]
Q9VEP19.4e-1569.23Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase 1 OS=Drosophil... [more]
Q9UET61.6e-1467.31Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien... [more]
Q220312.7e-1471.15Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi... [more]
BLAST of CmoCh06G002330 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GES53.5e-20100.00putative tRNA (Cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m... [more]
A0A067EPX45.5e-1883.05Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Citrus sine... [more]
A0A1U7WEB49.4e-1892.31Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Nicotiana s... [more]
A0A6I9T3P29.4e-1892.31Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Sesamum ind... [more]
A0A2P5YEP29.4e-1892.31Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Gossypium b... [more]
BLAST of CmoCh06G002330 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G01230.21.8e-2192.31S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G01230.11.8e-2192.31S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G25730.12.4e-0539.62FtsJ-like methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002877Ribosomal RNA methyltransferase FtsJ domainPFAMPF01728FtsJcoord: 21..53
e-value: 6.1E-9
score: 36.3
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 18..56
e-value: 4.7E-10
score: 41.5
NoneNo IPR availablePANTHERPTHR10920:SF25TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATEDcoord: 1..53
NoneNo IPR availablePANTHERPTHR10920RIBOSOMAL RNA METHYLTRANSFERASEcoord: 1..53

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G002330.1CmoCh06G002330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002181 cytoplasmic translation
biological_process GO:0002128 tRNA nucleoside ribose methylation
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008175 tRNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity