CmoCh05G013730 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G013730
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Description6-phosphogluconate dehydrogenase family protein
LocationCmo_Chr05: 10642562 .. 10643611 (+)
RNA-Seq ExpressionCmoCh05G013730
SyntenyCmoCh05G013730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCACTGCAAATCTCTCTTCGATCCCTATACACTCAATCCCACAAACTAAACCCCACCCTATCCCTTCCCTTCCTCCCTTACCTCCGCCGCACAATGGCCACTTCCGGCGACATCGTCAGCCCCACAACCACTCGCGTCGGCTGGATCGGCACCGGCGTTATGGGCTGCTCCATGTGCTCTCACATCATCAAAGCCGGCTTCAAACTCACCGTCTTTAATCGCACAATCTCGAAAGCCCAACCCCTTCTCGATTTGGGCGCCAATTTGGCCTCTTCCCCTCTCGCCGTCGCCGCCCAATCCGACGTCGTTTTCTCCATTGTCGGTTACCCCTCCGACGTCCGTTCCGTCCTCCTCGATCCCACCTCCGGCGCCCTAGCCGGCCTCCGCCCCGGCGGCGTCCTCGTAGATATGACCACTTCCGAACCCTCACTCGCTTCTGAAATCGCCGCGGCCACTGCCGCCAAGGGCTGCAAGGCAGTTGACGCTCCGGTTTCGGGCGGCGATCGCGGCGCAAAGAACGCTTCTCTGGCGATCTTTGCCGGCGGTAACGAAGGCGAAGTCCGGCGATTGAACCCATTATTCTCCCTGTTGGGGAAAGTGAATTACATGGGGGAGAGTGGGAAAGGGCAATTTGCGAAGCTGGCGAATCAGATCACCATAGCTTCAACGATGGTGGGGCTAGTGGAAGGGATGATTTACGCTCACAAAGCAGGGCTTGATGTGAGTTTGTTCTTGAACGCCATATCCACAGGGGCAGCAGGATCAAAATCGCTGGATTTGTATGGGAGTAGGATTTTGCAGAGGGATTTTGAACCTGGGTTTTTCGTGAACCATTTCGTGAAGGATTTAGGAATTTGCTTGAAGGAATGTCAGAATATGGGGCTCGCCCTTCCTGGACTGGCATTGGCTCAGCAGCTTTATGTGTCGCTCAAGGCTCATGGGGAGGGGAATTTGGGCACTCAGGCCCTGATTTTATCCCTGGAACGGCTGAATAATGTTTCACTCCAATCTGCCGCCCGCGGTGGCAGTGGCAGCGGTTCTTCTTAA

mRNA sequence

ATGCCACTGCAAATCTCTCTTCGATCCCTATACACTCAATCCCACAAACTAAACCCCACCCTATCCCTTCCCTTCCTCCCTTACCTCCGCCGCACAATGGCCACTTCCGGCGACATCGTCAGCCCCACAACCACTCGCGTCGGCTGGATCGGCACCGGCGTTATGGGCTGCTCCATGTGCTCTCACATCATCAAAGCCGGCTTCAAACTCACCGTCTTTAATCGCACAATCTCGAAAGCCCAACCCCTTCTCGATTTGGGCGCCAATTTGGCCTCTTCCCCTCTCGCCGTCGCCGCCCAATCCGACGTCGTTTTCTCCATTGTCGGTTACCCCTCCGACGTCCGTTCCGTCCTCCTCGATCCCACCTCCGGCGCCCTAGCCGGCCTCCGCCCCGGCGGCGTCCTCGTAGATATGACCACTTCCGAACCCTCACTCGCTTCTGAAATCGCCGCGGCCACTGCCGCCAAGGGCTGCAAGGCAGTTGACGCTCCGGTTTCGGGCGGCGATCGCGGCGCAAAGAACGCTTCTCTGGCGATCTTTGCCGGCGGTAACGAAGGCGAAGTCCGGCGATTGAACCCATTATTCTCCCTGTTGGGGAAAGTGAATTACATGGGGGAGAGTGGGAAAGGGCAATTTGCGAAGCTGGCGAATCAGATCACCATAGCTTCAACGATGGTGGGGCTAGTGGAAGGGATGATTTACGCTCACAAAGCAGGGCTTGATGTGAGTTTGTTCTTGAACGCCATATCCACAGGGGCAGCAGGATCAAAATCGCTGGATTTGTATGGGAGTAGGATTTTGCAGAGGGATTTTGAACCTGGGTTTTTCGTGAACCATTTCGTGAAGGATTTAGGAATTTGCTTGAAGGAATGTCAGAATATGGGGCTCGCCCTTCCTGGACTGGCATTGGCTCAGCAGCTTTATGTGTCGCTCAAGGCTCATGGGGAGGGGAATTTGGGCACTCAGGCCCTGATTTTATCCCTGGAACGGCTGAATAATGTTTCACTCCAATCTGCCGCCCGCGGTGGCAGTGGCAGCGGTTCTTCTTAA

Coding sequence (CDS)

ATGCCACTGCAAATCTCTCTTCGATCCCTATACACTCAATCCCACAAACTAAACCCCACCCTATCCCTTCCCTTCCTCCCTTACCTCCGCCGCACAATGGCCACTTCCGGCGACATCGTCAGCCCCACAACCACTCGCGTCGGCTGGATCGGCACCGGCGTTATGGGCTGCTCCATGTGCTCTCACATCATCAAAGCCGGCTTCAAACTCACCGTCTTTAATCGCACAATCTCGAAAGCCCAACCCCTTCTCGATTTGGGCGCCAATTTGGCCTCTTCCCCTCTCGCCGTCGCCGCCCAATCCGACGTCGTTTTCTCCATTGTCGGTTACCCCTCCGACGTCCGTTCCGTCCTCCTCGATCCCACCTCCGGCGCCCTAGCCGGCCTCCGCCCCGGCGGCGTCCTCGTAGATATGACCACTTCCGAACCCTCACTCGCTTCTGAAATCGCCGCGGCCACTGCCGCCAAGGGCTGCAAGGCAGTTGACGCTCCGGTTTCGGGCGGCGATCGCGGCGCAAAGAACGCTTCTCTGGCGATCTTTGCCGGCGGTAACGAAGGCGAAGTCCGGCGATTGAACCCATTATTCTCCCTGTTGGGGAAAGTGAATTACATGGGGGAGAGTGGGAAAGGGCAATTTGCGAAGCTGGCGAATCAGATCACCATAGCTTCAACGATGGTGGGGCTAGTGGAAGGGATGATTTACGCTCACAAAGCAGGGCTTGATGTGAGTTTGTTCTTGAACGCCATATCCACAGGGGCAGCAGGATCAAAATCGCTGGATTTGTATGGGAGTAGGATTTTGCAGAGGGATTTTGAACCTGGGTTTTTCGTGAACCATTTCGTGAAGGATTTAGGAATTTGCTTGAAGGAATGTCAGAATATGGGGCTCGCCCTTCCTGGACTGGCATTGGCTCAGCAGCTTTATGTGTCGCTCAAGGCTCATGGGGAGGGGAATTTGGGCACTCAGGCCCTGATTTTATCCCTGGAACGGCTGAATAATGTTTCACTCCAATCTGCCGCCCGCGGTGGCAGTGGCAGCGGTTCTTCTTAA

Protein sequence

MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS
Homology
BLAST of CmoCh05G013730 vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.8e-135
Identity = 244/315 (77.46%), Postives = 282/315 (89.52%), Query Frame = 0

Query: 28  YLRRTMAT---SGDIVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLL 87
           +LRR+MA+   S DI++P+ T++GWIGTGVMG SMC H+IKAG+ +TVFNRTISKAQ L+
Sbjct: 18  FLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLI 77

Query: 88  DLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPS 147
           D+GAN+A SP +VA QSDVVF+IVGYPSDVR VLLDP SGAL+GLR GGVLVDMTTSEPS
Sbjct: 78  DMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPS 137

Query: 148 LASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYM 207
           LA EIA A + K C ++DAPVSGGD GAKN  L+IFAGG+E  V+RL+PLFSL+GKVN+M
Sbjct: 138 LAEEIAKAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFM 197

Query: 208 GESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGS 267
           G SGKGQFAKLANQITIASTM+GLVEG+IYAHKAGLDV  FL AISTGAAGSKS+DLYG 
Sbjct: 198 GTSGKGQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGD 257

Query: 268 RILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQAL 327
           RIL+RDF+PGF+VNHFVKDLGICL ECQ MGLALPGLALAQQLY+SLKAHGEG+LGTQAL
Sbjct: 258 RILKRDFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQAL 317

Query: 328 ILSLERLNNVSLQSA 340
           +L+LERLNNVS+QS+
Sbjct: 318 LLALERLNNVSVQSS 332

BLAST of CmoCh05G013730 vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.9e-71
Identity = 144/297 (48.48%), Postives = 198/297 (66.67%), Query Frame = 0

Query: 39  IVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVA 98
           ++ P+ TR+GWIG G+MG +M SHII AG+ +TV+ R + K + L   GA +A+SP  +A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL PGGV VDMT+S+P LA EI A    + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 159 KAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQ 218
            AVDAPVSGGD GA+  +L IFAGG+   V  L+P+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 219 ITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVN 278
           I  AS +VGL EG+++A KAGLD   +L A+  GAAGS  + L+G  I++RD+    F  
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +       A+PG AL++QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

BLAST of CmoCh05G013730 vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.5e-67
Identity = 137/297 (46.13%), Postives = 192/297 (64.65%), Query Frame = 0

Query: 39  IVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVA 98
           ++ P+ TR+GWIG G+MG +M SHI+ AG+ +TV+ R + K + L   G   A+SP  + 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL+PGGV VDMT+S+P LA EI A    + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 159 KAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQ 218
            AVDAPVSGGD GA+   L IFAGG+   V  L P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 219 ITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVN 278
           I + S M+GL EG+++A KAGLD   +L A+  GAAGS  + L+G  +  RD++   F  
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +      +A+PG AL +QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

BLAST of CmoCh05G013730 vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.0e-64
Identity = 133/279 (47.67%), Postives = 184/279 (65.95%), Query Frame = 0

Query: 47  VGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFS 106
           +G+IG GVMG SM SHI+  G  + V+ RT  KA+ +L  GA    +   ++ ++DV+ +
Sbjct: 5   IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIIT 64

Query: 107 IVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVS 166
           +VGYPSDV  V    ++G +   + G  L+DMTTS+PSLA +IA A   K   A+DAPVS
Sbjct: 65  MVGYPSDVEEVYFG-SNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVS 124

Query: 167 GGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGK-VNYMGESGKGQFAKLANQITIASTM 226
           GGD GA+N +LAI  GG +       P+FSL+G+ + Y G +G GQ  K+ NQI IA+ M
Sbjct: 125 GGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAAGM 184

Query: 227 VGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLG 286
           +G+ E M YA K+GL+    L +I+TGAAGS SL     R+LQ +FEPGF+V HF+KD+G
Sbjct: 185 IGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKDMG 244

Query: 287 ICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQAL 325
           I L+E + MG  +PGL+LA+ LY  L A GE N GTQ++
Sbjct: 245 IALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSI 282

BLAST of CmoCh05G013730 vs. ExPASy Swiss-Prot
Match: O33730 (Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=Sfri_1503 PE=3 SV=2)

HSP 1 Score: 169.9 bits (429), Expect = 5.2e-41
Identity = 107/287 (37.28%), Postives = 153/287 (53.31%), Query Frame = 0

Query: 46  RVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKA-QPLLDLGANLASSPLAVAAQSDVV 105
           +V +IG GVMG  M  H++K G  +TV+NRT +KA Q +   G   A +P   A   D+V
Sbjct: 3   KVAFIGLGVMGFPMAGHLVKQGHDVTVYNRTGAKATQWVEQYGGKKADTPKDAAQGQDIV 62

Query: 106 FSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAP 165
           F+ VG   D+R V+L    G + G+  G +LVD TT+   +A EIAA         +DAP
Sbjct: 63  FTCVGNDDDLRQVVLG-EHGIVHGMHAGAILVDHTTASADVAREIAAYIEPLNIAFLDAP 122

Query: 166 VSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGK-VNYMGESGKGQFAKLANQITIAS 225
           VSGG  GA+N +L +  GG++     + P+ S   +    +G  G GQ  K+ NQI IA 
Sbjct: 123 VSGGQAGAENGALTVMMGGDQAHFDTVKPVISAYSRCAELLGPVGAGQLTKMVNQICIAG 182

Query: 226 TMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKD 285
            + GL EG+ +A  AGLD    +  IS GAA S  ++     + Q  ++ GF ++   KD
Sbjct: 183 VVQGLAEGLHFAKSAGLDGLKVIEVISKGAAQSWQMENRYKTMWQGQYDFGFAIDWMRKD 242

Query: 286 LGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLER 331
           LGI L E +  G  LP  AL  Q Y  ++A       T +L+  LE+
Sbjct: 243 LGIALDEARRNGSHLPVAALVDQFYSEVQAMKGNRWDTSSLLARLEK 288

BLAST of CmoCh05G013730 vs. ExPASy TrEMBL
Match: A0A6J1G2N8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111450245 PE=4 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 3.9e-188
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS 350
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS 349

BLAST of CmoCh05G013730 vs. ExPASy TrEMBL
Match: A0A6J1KJW1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111493847 PE=4 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.4e-184
Identity = 342/348 (98.28%), Postives = 345/348 (99.14%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQISLRSLYTQSHKL PTLSL FLP+LRRTMATSGDIV+PTTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLAPTLSLSFLPHLRRTMATSGDIVNPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGFKLTVFNRTISKAQPL+DLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLVDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAA AAKGCKAVDAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGS 349
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGS 348

BLAST of CmoCh05G013730 vs. ExPASy TrEMBL
Match: A0A1S3C8W8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498120 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 9.6e-171
Identity = 317/346 (91.62%), Postives = 330/346 (95.38%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQ +LRSLYTQS KL PTLSLPFL +LRRTM+TSGDIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQFTLRSLYTQSQKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAG+KLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAA  AKGC A+DAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+EGEVRRL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGDEGEVRRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGS 347
           LALAQQLYVSLKA+GEGNLGTQALILS+ERLNNVSL       +GS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSVERLNNVSLDDGGSRANGS 346

BLAST of CmoCh05G013730 vs. ExPASy TrEMBL
Match: A0A0A0LIQ8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000840 PE=4 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.4e-169
Identity = 316/349 (90.54%), Postives = 333/349 (95.42%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MP+Q SLRSLYTQSHKL PTLSLPFL +LRRTM+TSGDIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPIQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAG+KLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           P+SGALAGLRPGGVL+DMTTSEPSLASEIAA+  AKGC A+DAPVSGGDRGAKNA+LAIF
Sbjct: 121 PSSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+E EV+RL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS 350
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSL+       GSGSS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLE-------GSGSS 342

BLAST of CmoCh05G013730 vs. ExPASy TrEMBL
Match: A0A6J1DMH9 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021913 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 6.6e-164
Identity = 308/344 (89.53%), Postives = 328/344 (95.35%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQISLRSL+  +H L  TLS+  +  LRRTMATSGDI+SP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLH--AHSL--TLSVQLVSLLRRTMATSGDILSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SH+I+AGFKLTVFNRTISKAQPLLDLGA+LASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGG+LVDMTTSEPSLASEIAAA AAKGC A+DAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+EG+VRRLNPLF+LLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFE GFFVNHFVKDLGICLKECQNMG+ALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVNHFVKDLGICLKECQNMGIALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGS 345
           LALAQQLY+SLKAHGEGNLGTQALILSLERLN+VSLQS+A   S
Sbjct: 301 LALAQQLYLSLKAHGEGNLGTQALILSLERLNDVSLQSSAAASS 340

BLAST of CmoCh05G013730 vs. NCBI nr
Match: XP_022946035.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 667.2 bits (1720), Expect = 8.0e-188
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS 350
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS 349

BLAST of CmoCh05G013730 vs. NCBI nr
Match: XP_023545872.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.9 bits (1696), Expect = 4.8e-185
Identity = 344/349 (98.57%), Postives = 346/349 (99.14%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTM TSGDIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMTTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAA AAKGCKAVDAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGNEGEV+RLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVQRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS 350
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAA GGSGSGSS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAAGGGSGSGSS 349

BLAST of CmoCh05G013730 vs. NCBI nr
Match: XP_022999508.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 653.7 bits (1685), Expect = 9.1e-184
Identity = 342/348 (98.28%), Postives = 345/348 (99.14%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQISLRSLYTQSHKL PTLSL FLP+LRRTMATSGDIV+PTTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLAPTLSLSFLPHLRRTMATSGDIVNPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGFKLTVFNRTISKAQPL+DLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLVDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAA AAKGCKAVDAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGS 349
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGS 348

BLAST of CmoCh05G013730 vs. NCBI nr
Match: XP_008458836.1 (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis melo])

HSP 1 Score: 609.4 bits (1570), Expect = 2.0e-170
Identity = 317/346 (91.62%), Postives = 330/346 (95.38%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQ +LRSLYTQS KL PTLSLPFL +LRRTM+TSGDIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQFTLRSLYTQSQKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAG+KLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAA  AKGC A+DAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+EGEVRRL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGDEGEVRRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGS 347
           LALAQQLYVSLKA+GEGNLGTQALILS+ERLNNVSL       +GS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSVERLNNVSLDDGGSRANGS 346

BLAST of CmoCh05G013730 vs. NCBI nr
Match: XP_038889572.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 609.0 bits (1569), Expect = 2.6e-170
Identity = 322/350 (92.00%), Postives = 334/350 (95.43%), Query Frame = 0

Query: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60
           MPLQISLRSLYTQS++L  TLSLPFL +LR TM+TS DIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSNQLTHTLSLPFLSFLRCTMSTSRDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSD+VFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDIVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAA  AKGC A+DAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+E EVRRL PLFSLLGKVNYMGE+GKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGDESEVRRLKPLFSLLGKVNYMGETGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSG-SGSS 350
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSL+SA  G  G SGSS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLESAPGGDGGRSGSS 350

BLAST of CmoCh05G013730 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 3.4e-136
Identity = 244/315 (77.46%), Postives = 282/315 (89.52%), Query Frame = 0

Query: 28  YLRRTMAT---SGDIVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLL 87
           +LRR+MA+   S DI++P+ T++GWIGTGVMG SMC H+IKAG+ +TVFNRTISKAQ L+
Sbjct: 18  FLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLI 77

Query: 88  DLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPS 147
           D+GAN+A SP +VA QSDVVF+IVGYPSDVR VLLDP SGAL+GLR GGVLVDMTTSEPS
Sbjct: 78  DMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPS 137

Query: 148 LASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYM 207
           LA EIA A + K C ++DAPVSGGD GAKN  L+IFAGG+E  V+RL+PLFSL+GKVN+M
Sbjct: 138 LAEEIAKAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFM 197

Query: 208 GESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGS 267
           G SGKGQFAKLANQITIASTM+GLVEG+IYAHKAGLDV  FL AISTGAAGSKS+DLYG 
Sbjct: 198 GTSGKGQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGD 257

Query: 268 RILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQAL 327
           RIL+RDF+PGF+VNHFVKDLGICL ECQ MGLALPGLALAQQLY+SLKAHGEG+LGTQAL
Sbjct: 258 RILKRDFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQAL 317

Query: 328 ILSLERLNNVSLQSA 340
           +L+LERLNNVS+QS+
Sbjct: 318 LLALERLNNVSVQSS 332

BLAST of CmoCh05G013730 vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 270.4 bits (690), Expect = 2.0e-72
Identity = 144/297 (48.48%), Postives = 198/297 (66.67%), Query Frame = 0

Query: 39  IVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVA 98
           ++ P+ TR+GWIG G+MG +M SHII AG+ +TV+ R + K + L   GA +A+SP  +A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL PGGV VDMT+S+P LA EI A    + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 159 KAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQ 218
            AVDAPVSGGD GA+  +L IFAGG+   V  L+P+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 219 ITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVN 278
           I  AS +VGL EG+++A KAGLD   +L A+  GAAGS  + L+G  I++RD+    F  
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +       A+PG AL++QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

BLAST of CmoCh05G013730 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.8e-68
Identity = 137/297 (46.13%), Postives = 192/297 (64.65%), Query Frame = 0

Query: 39  IVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVA 98
           ++ P+ TR+GWIG G+MG +M SHI+ AG+ +TV+ R + K + L   G   A+SP  + 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL+PGGV VDMT+S+P LA EI A    + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 159 KAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQ 218
            AVDAPVSGGD GA+   L IFAGG+   V  L P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 219 ITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVN 278
           I + S M+GL EG+++A KAGLD   +L A+  GAAGS  + L+G  +  RD++   F  
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +      +A+PG AL +QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

BLAST of CmoCh05G013730 vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 139.0 bits (349), Expect = 7.0e-33
Identity = 91/312 (29.17%), Postives = 154/312 (49.36%), Query Frame = 0

Query: 19  PTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTIS 78
           P + L +  Y      T        T  +G++G G+MG  M  ++IKAG  +TV+NRT S
Sbjct: 41  PQICLAYRVYSSLQSTTPSTRDELGTVSIGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKS 100

Query: 79  KAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDM 138
           K  PL+ LGA   SSP  V A  D+ F+++  P     V     +GA+ G+  G   VD+
Sbjct: 101 KCDPLVGLGAKYKSSPEEVTATCDLTFAMLADPESAIDVACG-KNGAIFGISSGKGYVDV 160

Query: 139 TTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLL 198
           +T + + +  I+      G   ++APVSG  + A++  L     G++    +  P   ++
Sbjct: 161 STVDVASSILISKQIKDTGALFLEAPVSGSKKPAEDGQLIFLTAGDKPLYEKAAPFLDIM 220

Query: 199 GKVN-YMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSK 258
           GK   Y+GE G G   KL   + + S M    EG++ + K GLD ++ +  +S GA  + 
Sbjct: 221 GKSKFYLGEVGNGAAMKLVVNMIMGSMMASFAEGILLSQKVGLDPNVLVEVVSQGAINAP 280

Query: 259 SLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEG 318
              L G  +++  +   F + H  KD+ + L   +++  + P  A A +LY   K++G  
Sbjct: 281 MYSLKGPSMIKSVYPTAFPLKHQQKDMRLALGLAESVSQSTPIAAAANELYKVAKSYGLS 340

Query: 319 NLGTQALILSLE 330
           +    A+I +L+
Sbjct: 341 DEDFSAVIEALK 351

BLAST of CmoCh05G013730 vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 132.5 bits (332), Expect = 6.6e-31
Identity = 85/280 (30.36%), Postives = 144/280 (51.43%), Query Frame = 0

Query: 47  VGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFS 106
           VG++G G+MG +M  +++K GFK+TV+NRT+SK   L++ GA++  SP  V  +     +
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 107 IVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVS 166
           ++  P    SV+ D   G L  +  G   +DM+T +   + +I  A   KG + V+ PVS
Sbjct: 63  MLSDPCAALSVVFD-KGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVS 122

Query: 167 GGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVN-YMGESGKGQFAKLANQITIASTM 226
           G  + A++  L I A G++       P F +LGK + Y+G+ G G   KL   + + S M
Sbjct: 123 GSKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMM 182

Query: 227 VGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLG 286
               EG++ A K+GL     L+ +  GA  +      G  + +  + P F + H  KD+ 
Sbjct: 183 NAFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMR 242

Query: 287 ICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALI 326
           + L       +++P  A A + +   ++ G G+L   A+I
Sbjct: 243 LALALGDENAVSMPVAAAANEAFKKARSLGLGDLDFSAVI 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZE14.8e-13577.46Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis... [more]
Q949M82.9e-7148.48Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis... [more]
Q9C9912.5e-6746.13Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis... [more]
O349482.0e-6447.67Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 ... [more]
O337305.2e-4137.28Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCI... [more]
Match NameE-valueIdentityDescription
A0A6J1G2N83.9e-188100.00probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita m... [more]
A0A6J1KJW14.4e-18498.28probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita m... [more]
A0A1S3C8W89.6e-17191.62probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis mel... [more]
A0A0A0LIQ81.4e-16990.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000840 PE=4 SV=1[more]
A0A6J1DMH96.6e-16489.53probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Momordica c... [more]
Match NameE-valueIdentityDescription
XP_022946035.18.0e-188100.00probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita mos... [more]
XP_023545872.14.8e-18598.57probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pep... [more]
XP_022999508.19.1e-18498.28probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita max... [more]
XP_008458836.12.0e-17091.62PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cu... [more]
XP_038889572.12.6e-17092.00probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Benincasa his... [more]
Match NameE-valueIdentityDescription
AT4G29120.13.4e-13677.466-phosphogluconate dehydrogenase family protein [more]
AT1G71180.12.0e-7248.486-phosphogluconate dehydrogenase family protein [more]
AT1G71170.11.8e-6846.136-phosphogluconate dehydrogenase family protein [more]
AT1G17650.17.0e-3329.17glyoxylate reductase 2 [more]
AT3G25530.16.6e-3130.36glyoxylate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0291543-hydroxyisobutyrate dehydrogenase, NAD-binding domainPFAMPF14833NAD_binding_11coord: 208..325
e-value: 1.6E-32
score: 112.3
IPR0061156-phosphogluconate dehydrogenase, NADP-bindingPFAMPF03446NAD_binding_2coord: 46..202
e-value: 2.4E-41
score: 141.6
NoneNo IPR availableGENE3D3.40.50.720coord: 40..205
e-value: 1.7E-45
score: 156.8
NoneNo IPR availablePANTHERPTHR430603-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATEDcoord: 39..334
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 206..335
e-value: 2.2E-37
score: 130.2
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 45..203
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 206..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G013730.1CmoCh05G013730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051287 NAD binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0016491 oxidoreductase activity