CmoCh05G012620 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G012620
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionCBL-interacting serine/threonine-protein kinase 6
LocationCmo_Chr05: 9803375 .. 9804673 (+)
RNA-Seq ExpressionCmoCh05G012620
SyntenyCmoCh05G012620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAATAAAGGCAGAGATGGTGAACACTCGAGTTTACTCGACGGGAAGTACGAATTCGGCCGTCTTTTAGGGCACGGGACCTTCGCTAAGGTTTACCATGCCCGGAATCTGCAAACGGGGAAGAGCGTTGCGATGAAAGTTGTTGGGAAGGAGAAGATCATCAAAATCGGCATGATGGAGCAGATCAAGAGAGAAATCTCTGTGATGAAAAGGGTGAAGCATCCGAATATTGTGGAGCTTCATGAGGTGATGGCGAGTAAATCGAAGATCTATTTTGCGATGGAGCTTGTTAGAGGCGGTGAACTGTTCTCCAAAATCGTGAAAGGTCGGTTGCGAGAGGATGTGGCTAGGGTTTACTTTCAGCAATTGATCTCTGCAATCGATTTCTGCCATAGCCGTGGTGTTTACCACCGCGATCTGAAACCGGAAAATCTGCTACTCGATCAGGACGGAAACCTTAAGGTCACCGATTTTGGCCTCACCGCCTTCTCGGAGCATTTGAAGCAAGACGGACTGTTGCACACGACATGCGGAACTCCGGCGTACGTTGCGCCGGAGGTTATGGGAAAGCAGGGATACGACGGGGCTAAGGCCGATCTCTGGTCCTGTGGAGTCATCCTGTACGTTCTTCTTGCCGGATACCTTCCATTCCAGGACGACAATATGGTGGCGATGTACCGGAAGATTTACAGAGGCGATTTCAGGTGTCCACCGTGGTTCTCTCCCGAAGCTCGCCGCCTTGTCACGAAGCTTCTAGATCCGAATCCGGTGACCAGAGTCGGTATCGCGAAGATCACATCGTCGTTATGGTTCAAGAAATCGGTACCGAAGAGTGTGATTACGAAGGAGCATGAAGAAATGGAGGAGAATTTGGCGAAATCGAATCAACCGGCGGAAACGTTGAATGCATTCCACATAATATCTCTATCGGAAGGGTTTGATTTGTCGCCGTTGTTCGAAGAGAAGAAGAGGGAGGAGAAAGAGGAGATTAGATTCTTGACTTCATCTCCGGCGAGCAGTGTGATATCGAAGCTTGAGGAAGTGGCGAAGGCAGTGAAGTTCGATGTGAGGAAGAGCGAGTCGTGGGTTCGATTGCAGGGGCAAGAGAAGGGGAGAAAGGGGAAACTAGCGATTGCGGTGGATATCTTTGCGATGACGCCGTCGTTTGTGGTGGTGGAGGTGAAGAAGGACAACGGAGACACTTTAGAATACAATCAATTCTGCAGGGAGGAGCTCCGGCCGGCATTGAAGGACATTGTTTGGACTTCTCCGACTGGGAGCTCGACCCCAGCTTGA

mRNA sequence

ATGGCGAATAAAGGCAGAGATGGTGAACACTCGAGTTTACTCGACGGGAAGTACGAATTCGGCCGTCTTTTAGGGCACGGGACCTTCGCTAAGGTTTACCATGCCCGGAATCTGCAAACGGGGAAGAGCGTTGCGATGAAAGTTGTTGGGAAGGAGAAGATCATCAAAATCGGCATGATGGAGCAGATCAAGAGAGAAATCTCTGTGATGAAAAGGGTGAAGCATCCGAATATTGTGGAGCTTCATGAGGTGATGGCGAGTAAATCGAAGATCTATTTTGCGATGGAGCTTGTTAGAGGCGGTGAACTGTTCTCCAAAATCGTGAAAGGTCGGTTGCGAGAGGATGTGGCTAGGGTTTACTTTCAGCAATTGATCTCTGCAATCGATTTCTGCCATAGCCGTGGTGTTTACCACCGCGATCTGAAACCGGAAAATCTGCTACTCGATCAGGACGGAAACCTTAAGGTCACCGATTTTGGCCTCACCGCCTTCTCGGAGCATTTGAAGCAAGACGGACTGTTGCACACGACATGCGGAACTCCGGCGTACGTTGCGCCGGAGGTTATGGGAAAGCAGGGATACGACGGGGCTAAGGCCGATCTCTGGTCCTGTGGAGTCATCCTGTACGTTCTTCTTGCCGGATACCTTCCATTCCAGGACGACAATATGGTGGCGATGTACCGGAAGATTTACAGAGGCGATTTCAGGTGTCCACCGTGGTTCTCTCCCGAAGCTCGCCGCCTTGTCACGAAGCTTCTAGATCCGAATCCGGTGACCAGAGTCGGTATCGCGAAGATCACATCGTCGTTATGGTTCAAGAAATCGGTACCGAAGAGTGTGATTACGAAGGAGCATGAAGAAATGGAGGAGAATTTGGCGAAATCGAATCAACCGGCGGAAACGTTGAATGCATTCCACATAATATCTCTATCGGAAGGGTTTGATTTGTCGCCGTTGTTCGAAGAGAAGAAGAGGGAGGAGAAAGAGGAGATTAGATTCTTGACTTCATCTCCGGCGAGCAGTGTGATATCGAAGCTTGAGGAAGTGGCGAAGGCAGTGAAGTTCGATGTGAGGAAGAGCGAGTCGTGGGTTCGATTGCAGGGGCAAGAGAAGGGGAGAAAGGGGAAACTAGCGATTGCGGTGGATATCTTTGCGATGACGCCGTCGTTTGTGGTGGTGGAGGTGAAGAAGGACAACGGAGACACTTTAGAATACAATCAATTCTGCAGGGAGGAGCTCCGGCCGGCATTGAAGGACATTGTTTGGACTTCTCCGACTGGGAGCTCGACCCCAGCTTGA

Coding sequence (CDS)

ATGGCGAATAAAGGCAGAGATGGTGAACACTCGAGTTTACTCGACGGGAAGTACGAATTCGGCCGTCTTTTAGGGCACGGGACCTTCGCTAAGGTTTACCATGCCCGGAATCTGCAAACGGGGAAGAGCGTTGCGATGAAAGTTGTTGGGAAGGAGAAGATCATCAAAATCGGCATGATGGAGCAGATCAAGAGAGAAATCTCTGTGATGAAAAGGGTGAAGCATCCGAATATTGTGGAGCTTCATGAGGTGATGGCGAGTAAATCGAAGATCTATTTTGCGATGGAGCTTGTTAGAGGCGGTGAACTGTTCTCCAAAATCGTGAAAGGTCGGTTGCGAGAGGATGTGGCTAGGGTTTACTTTCAGCAATTGATCTCTGCAATCGATTTCTGCCATAGCCGTGGTGTTTACCACCGCGATCTGAAACCGGAAAATCTGCTACTCGATCAGGACGGAAACCTTAAGGTCACCGATTTTGGCCTCACCGCCTTCTCGGAGCATTTGAAGCAAGACGGACTGTTGCACACGACATGCGGAACTCCGGCGTACGTTGCGCCGGAGGTTATGGGAAAGCAGGGATACGACGGGGCTAAGGCCGATCTCTGGTCCTGTGGAGTCATCCTGTACGTTCTTCTTGCCGGATACCTTCCATTCCAGGACGACAATATGGTGGCGATGTACCGGAAGATTTACAGAGGCGATTTCAGGTGTCCACCGTGGTTCTCTCCCGAAGCTCGCCGCCTTGTCACGAAGCTTCTAGATCCGAATCCGGTGACCAGAGTCGGTATCGCGAAGATCACATCGTCGTTATGGTTCAAGAAATCGGTACCGAAGAGTGTGATTACGAAGGAGCATGAAGAAATGGAGGAGAATTTGGCGAAATCGAATCAACCGGCGGAAACGTTGAATGCATTCCACATAATATCTCTATCGGAAGGGTTTGATTTGTCGCCGTTGTTCGAAGAGAAGAAGAGGGAGGAGAAAGAGGAGATTAGATTCTTGACTTCATCTCCGGCGAGCAGTGTGATATCGAAGCTTGAGGAAGTGGCGAAGGCAGTGAAGTTCGATGTGAGGAAGAGCGAGTCGTGGGTTCGATTGCAGGGGCAAGAGAAGGGGAGAAAGGGGAAACTAGCGATTGCGGTGGATATCTTTGCGATGACGCCGTCGTTTGTGGTGGTGGAGGTGAAGAAGGACAACGGAGACACTTTAGAATACAATCAATTCTGCAGGGAGGAGCTCCGGCCGGCATTGAAGGACATTGTTTGGACTTCTCCGACTGGGAGCTCGACCCCAGCTTGA

Protein sequence

MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKSESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDIVWTSPTGSSTPA
Homology
BLAST of CmoCh05G012620 vs. ExPASy Swiss-Prot
Match: O65554 (CBL-interacting serine/threonine-protein kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CIPK6 PE=1 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 2.4e-189
Identity = 328/430 (76.28%), Postives = 370/430 (86.05%), Query Frame = 0

Query: 3   NKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQ 62
           N    G  + LL G+YE GRLLGHGTFAKVYHARN+QTGKSVAMKVVGKEK++K+GM++Q
Sbjct: 9   NGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQ 68

Query: 63  IKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQ 122
           IKREISVM+ VKHPNIVELHEVMASKSKIYFAMELVRGGELF+K+ KGRLREDVARVYFQ
Sbjct: 69  IKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQ 128

Query: 123 QLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPA 182
           QLISA+DFCHSRGVYHRDLKPENLLLD++GNLKVTDFGL+AF+EHLKQDGLLHTTCGTPA
Sbjct: 129 QLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPA 188

Query: 183 YVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFS 242
           YVAPEV+ K+GYDGAKADLWSCGVIL+VLLAGYLPFQDDN+V MYRKIYRGDF+CP W S
Sbjct: 189 YVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLS 248

Query: 243 PEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKS-------VITKEHEEMEENLAKS 302
            +ARRLVTKLLDPNP TR+ I K+  S WFKK   +S        IT   E+++  + KS
Sbjct: 249 SDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKS 308

Query: 303 NQPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAK-AVK 362
            +  ETLNAFHII+LSEGFDLSPLFEEKK+EEK E+RF TS PASSVIS LEE A+   K
Sbjct: 309 KEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNK 368

Query: 363 FDVRKSESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELR 422
           FDVRKSES VR++G++ GRKGKLA+  +IFA+ PSFVVVEVKKD+GDTLEYN FC   LR
Sbjct: 369 FDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALR 428

Query: 423 PALKDIVWTS 425
           PALKDI WTS
Sbjct: 429 PALKDIFWTS 438

BLAST of CmoCh05G012620 vs. ExPASy Swiss-Prot
Match: Q9LWM4 (CBL-interacting protein kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK5 PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 5.7e-154
Identity = 263/431 (61.02%), Postives = 338/431 (78.42%), Query Frame = 0

Query: 11  SSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQIKREISVM 70
           +S+L  +YE GR+LG GTFAKVYHARNL + +SVA+KV+ KEK++++GM++QIKREIS+M
Sbjct: 5   ASILMNRYELGRMLGQGTFAKVYHARNLASNQSVAIKVIDKEKVLRVGMIDQIKREISIM 64

Query: 71  KRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQQLISAIDF 130
           + V+HPNIV+LHEVMASKSKIYFAME VRGGELFS++ +GRL+ED AR YFQQLI A+DF
Sbjct: 65  RLVRHPNIVQLHEVMASKSKIYFAMEYVRGGELFSRVARGRLKEDAARKYFQQLIGAVDF 124

Query: 131 CHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVMG 190
           CHSRGVYHRDLKPENLL+D++GNLKV+DFGL+AF E  KQDGLLHTTCGTPAYVAPE++ 
Sbjct: 125 CHSRGVYHRDLKPENLLVDENGNLKVSDFGLSAFKECQKQDGLLHTTCGTPAYVAPEIIN 184

Query: 191 KQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFSPEARRLVT 250
           K+GYDGAKAD+WSCGVIL+VLLAGYLPF D N++ MYRKI +GD + P WF+ + RRL++
Sbjct: 185 KRGYDGAKADIWSCGVILFVLLAGYLPFHDSNLMEMYRKISKGDVKFPQWFTTDVRRLLS 244

Query: 251 KLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEM--------------EENLAKSN 310
           +LLDPNP  R+ + K+    WFKK    +V+  +  E               ++N  K+ 
Sbjct: 245 RLLDPNPNIRITVEKLVEHPWFKKGYKPAVMLSQPNESNNLKDVHTAFSADHKDNEGKAK 304

Query: 311 QPAE-----TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAK 370
           +PA      +LNAF IISLS+GFDLS LFE  K E+K + RF+T  PAS+++SKLE++A+
Sbjct: 305 EPASSLKPVSLNAFDIISLSKGFDLSGLFENDK-EQKADSRFMTQKPASAIVSKLEQIAE 364

Query: 371 AVKFDVRKSESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCRE 423
              F V+K +  V+LQG ++GRKG+LAI  +IF +TPSF VVEVKK  GDTLEY +FC +
Sbjct: 365 TESFKVKKQDGLVKLQGSKEGRKGQLAIDAEIFEVTPSFFVVEVKKSAGDTLEYEKFCNK 424

BLAST of CmoCh05G012620 vs. ExPASy Swiss-Prot
Match: Q6Z9F4 (CBL-interacting protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK6 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 9.4e-149
Identity = 269/438 (61.42%), Postives = 335/438 (76.48%), Query Frame = 0

Query: 1   MANKGRDGE----HSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIK 60
           MA    +GE      ++L G+YE GR+LGHG F +V+ AR+L+TG+SVA+KVV KEK+++
Sbjct: 2   MAAAAEEGEGKKGGGTVLQGRYEMGRVLGHGNFGRVHVARDLRTGRSVAVKVVAKEKVVR 61

Query: 61  IGMMEQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVK-GRLRED 120
            GMMEQIKREI+VMKRV HPNIVELHEVMA++SKIY A+ELVRGGELF +IV+ GR+RED
Sbjct: 62  AGMMEQIKREIAVMKRVSHPNIVELHEVMATRSKIYLALELVRGGELFGRIVRLGRVRED 121

Query: 121 VARVYFQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLH 180
            AR YF+QL+SA+DFCHSRGVYHRDLKPENLLLD+ GNLKV DFGL+A ++H + DGLLH
Sbjct: 122 AARHYFRQLVSAVDFCHSRGVYHRDLKPENLLLDEAGNLKVVDFGLSALADHARADGLLH 181

Query: 181 TTCGTPAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDF 240
           T CGTP Y APEV+  +GYDGAKADLWSCGVILYVLLAG LPF DDN+V +YRK  RGD+
Sbjct: 182 TLCGTPGYAAPEVLRDKGYDGAKADLWSCGVILYVLLAGSLPFPDDNIVTLYRKAQRGDY 241

Query: 241 RCPPWFSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKS 300
           RCP W S +ARRL+ +LLDPNP TR+ +A++  + WFKK+     ++ E      + A +
Sbjct: 242 RCPAWLSTDARRLIPRLLDPNPTTRISVAQLVETPWFKKTSISRPVSIELPPAFADPAPA 301

Query: 301 NQPA-----ETLNAFHIISLSEGFDLSPLFEEKKREEKEE--IRFLTSSPASSVISKLEE 360
            + A     ETLNAFH+ISLSEGFDLSPLFE    + + +  + F T  PAS VIS+LE 
Sbjct: 302 KEEAEKDEPETLNAFHLISLSEGFDLSPLFEGDSAKGRRDGGMLFATREPASGVISRLEG 361

Query: 361 VAKAVKFDVRKSES---WVRLQGQEK-GRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLE 420
           VA      +R ++S    VRL+G E+ G KG+LA+A DIF++ PS +VV+VKKD GDTLE
Sbjct: 362 VAARGGGRMRVTKSGARGVRLEGAERGGAKGRLAVAADIFSVAPSVLVVDVKKDGGDTLE 421

Query: 421 YNQFCREELRPALKDIVW 423
           Y  FC EELRPAL+DIVW
Sbjct: 422 YRSFCSEELRPALQDIVW 439

BLAST of CmoCh05G012620 vs. ExPASy Swiss-Prot
Match: Q5W736 (CBL-interacting protein kinase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK18 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 7.9e-148
Identity = 259/437 (59.27%), Postives = 334/437 (76.43%), Query Frame = 0

Query: 9   EHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQIKREIS 68
           ++ ++L  +YE G+LLG GTFAKVYH RN+ T +SVA+KV+ K+KI K+G+M+QIKREIS
Sbjct: 6   KNGNILLRRYEIGKLLGQGTFAKVYHGRNIVTSQSVAIKVIDKDKIFKVGLMDQIKREIS 65

Query: 69  VMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQQLISAI 128
           VMK V+HPNIV+L+EVMA+KSKIYF +E V+GGELF+K+ KGRL+ED AR YFQQL+SA+
Sbjct: 66  VMKLVRHPNIVQLYEVMATKSKIYFVLEYVKGGELFNKVAKGRLKEDAARKYFQQLVSAV 125

Query: 129 DFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEV 188
           DFCHSRGVYHRDLKPENLL+D++GNLK+TDFGL+A +E  +QDGLLHTTCGTPAYVAPEV
Sbjct: 126 DFCHSRGVYHRDLKPENLLVDENGNLKITDFGLSALAESRRQDGLLHTTCGTPAYVAPEV 185

Query: 189 MGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFSPEARRL 248
           + ++GYDG K D WSCGVIL+VL+AGYLPFQD N++ MYRKI + +F+CP WFS + R+L
Sbjct: 186 ISRKGYDGVKVDTWSCGVILFVLMAGYLPFQDSNLMEMYRKIGKAEFKCPAWFSSDVRKL 245

Query: 249 VTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLA-------------KS 308
           V+++LDPNP +R+ I KI  + WFKK +   +I K  E  E   A              S
Sbjct: 246 VSRILDPNPRSRMPITKIMETYWFKKGLDSKLILKNVETNEPVTALADVNVVFSSMGSSS 305

Query: 309 NQPAE---------TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKL 368
           ++  E          LNAF IISLSEGFDLS LFEE   ++K+E RF +S  AS++ISKL
Sbjct: 306 SKKTEEKQDAGKLTNLNAFDIISLSEGFDLSGLFEE--TDKKKEARFTSSQSASAIISKL 365

Query: 369 EEVAKAVKFDVRKSESWV-RLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEY 423
           E+VA   K  V+K E  V +++G  +GRKG LAI  +IF +TPSF +VE+KK+NGDTLEY
Sbjct: 366 EDVASCSKLTVKKKEGGVLKMEGASEGRKGVLAIDAEIFEVTPSFHLVEIKKNNGDTLEY 425

BLAST of CmoCh05G012620 vs. ExPASy Swiss-Prot
Match: Q9FJ54 (CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CIPK20 PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 1.8e-144
Identity = 250/414 (60.39%), Postives = 327/414 (78.99%), Query Frame = 0

Query: 17  KYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQIKREISVMKRVKHP 76
           KYE GRLLG GTFAKVYHARN++TG+SVA+KV+ K+K+ K+G+++QIKREISVM+ V+HP
Sbjct: 11  KYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRLVRHP 70

Query: 77  NIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQQLISAIDFCHSRGV 136
           ++V LHEVMASK+KIYFAME V+GGELF K+ KG+L+E++AR YFQQLI AID+CHSRGV
Sbjct: 71  HVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIGAIDYCHSRGV 130

Query: 137 YHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVMGKQGYDG 196
           YHRDLKPENLLLD++G+LK++DFGL+A  E  +QDGLLHTTCGTPAYVAPEV+GK+GYDG
Sbjct: 131 YHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGKKGYDG 190

Query: 197 AKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFSPEARRLVTKLLDPN 256
           AKAD+WSCGV+LYVLLAG+LPF + N+V MYRKI +G+F+CP WF PE ++L++++LDPN
Sbjct: 191 AKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSRILDPN 250

Query: 257 PVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE------TLNAFHII-S 316
           P +R+ I KI  + WF+K   K    K  E  + +   S+  A       + NAF +I S
Sbjct: 251 PNSRIKIEKIMENSWFQKGFKKIETPKSPESHQIDSLISDVHAAFSVKPMSYNAFDLISS 310

Query: 317 LSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVA-KAVKFDVRKSESWVRLQG 376
           LS+GFDLS LFE   +EE+ E +F T   A  ++SK EE+A  + +F++ KS+  V+++ 
Sbjct: 311 LSQGFDLSGLFE---KEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVGVKMED 370

Query: 377 QEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDIVW 423
           + +GRKG LAI V+IF +T SF +VE KK  GDT+EY QFC  ELRP+LKDIVW
Sbjct: 371 KREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVW 421

BLAST of CmoCh05G012620 vs. ExPASy TrEMBL
Match: A0A6J1G4G9 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111450662 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.7e-247
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM
Sbjct: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY
Sbjct: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW
Sbjct: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE 300
           FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE
Sbjct: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE 300

Query: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS 360
           TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS
Sbjct: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS 360

Query: 361 ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420
           ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI
Sbjct: 361 ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420

Query: 421 VWTSPTGSSTPA 433
           VWTSPTGSSTPA
Sbjct: 421 VWTSPTGSSTPA 432

BLAST of CmoCh05G012620 vs. ExPASy TrEMBL
Match: A0A6J1KAP0 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111493756 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.1e-244
Identity = 426/432 (98.61%), Postives = 428/432 (99.07%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM
Sbjct: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMK VKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW
Sbjct: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE 300
           FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPK+VITKE EEMEENLAKSNQPAE
Sbjct: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKTVITKEQEEMEENLAKSNQPAE 300

Query: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS 360
           TLNAFHIISLSEGFDLSPLFEEKKREEKEE+RF TSSPASSVISKLEEVAKAVKFDVRKS
Sbjct: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVAKAVKFDVRKS 360

Query: 361 ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420
           ESWVRLQGQEKGRKGKLAIA DIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI
Sbjct: 361 ESWVRLQGQEKGRKGKLAIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420

Query: 421 VWTSPTGSSTPA 433
           VWTSPTGSSTPA
Sbjct: 421 VWTSPTGSSTPA 432

BLAST of CmoCh05G012620 vs. ExPASy TrEMBL
Match: A0A6J1HPX1 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111465554 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 9.5e-229
Identity = 396/433 (91.45%), Postives = 417/433 (96.30%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MA+KGRDG +SSLLDGKYE GRLLGHGTFAKVYHARNL+TGK+VAMKVVGKEKIIK+GM 
Sbjct: 1   MADKGRDGGYSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKTVAMKVVGKEKIIKVGMT 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMK VKHPNIVELHEVMASKSKIYFAME VRGGELF+KI KGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFTKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLD+DGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEV+GKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMY+KIYRGDF+CPPW
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYKKIYRGDFKCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEE-MEENLAKSNQPA 300
           FS EARRLVTKLLDPNPVTR+GI KITSS WFKKSVPK++ITKE +E +EEN  KS QPA
Sbjct: 241 FSSEARRLVTKLLDPNPVTRIGITKITSSSWFKKSVPKAIITKEEQERIEENFTKSKQPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRK 360
           ETLNAFHIISLSEGFDLSPLFEEKKRE+KEE+RF TSSPASSVISKLEEVAKAVKFDV+K
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREDKEEMRFATSSPASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKD 420
           SESWVRLQGQEKGRKGKLAIA DIFA+TPSFVVVEVKKDNGDTLEYNQFCR+ELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLAIAADIFALTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGSSTPA 433
           IVWTSPTG+STPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of CmoCh05G012620 vs. ExPASy TrEMBL
Match: A0A6J1FDM5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111444420 PE=3 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 1.6e-228
Identity = 396/433 (91.45%), Postives = 416/433 (96.07%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MA+KGRDG HSSLLDGKYE GRLLGHGTFAKVYHARNL+TGK+VAMKVVGKEKII++GM 
Sbjct: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKTVAMKVVGKEKIIRVGMT 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMK VKHPNIVELHEVMASKSKIYFAME VRGGELF+KI KGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFTKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLD+DGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEV+GKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMY+KIYRGDF+CPPW
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYKKIYRGDFKCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEE-MEENLAKSNQPA 300
           FS EARRLVTKLLDPNPVTR+GI KITSS WFKKSVPKS+ITKE +E +EEN  KS QPA
Sbjct: 241 FSSEARRLVTKLLDPNPVTRIGITKITSSSWFKKSVPKSIITKEEQEGIEENFTKSKQPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRK 360
           ETLNAFHIISLSEGFDLSPLFEEKKRE+KEE+RF TSSPASSVISKLEEVAKAVKFDV+K
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREDKEEMRFATSSPASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKD 420
           SESWVRLQGQEKGRKGKLAIA DIFA+TPSFVVVEVKKDNGDTLEYNQFCR+ELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLAIAADIFALTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGSSTPA 433
           IVWTSPT +STPA
Sbjct: 421 IVWTSPTENSTPA 433

BLAST of CmoCh05G012620 vs. ExPASy TrEMBL
Match: A0A0A0LL73 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_2G009490 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.2e-226
Identity = 395/433 (91.22%), Postives = 410/433 (94.69%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MA+KGRDG HSSLLDGKYE GRLLGHGTFAKVYHARNL+TGKSVAMKVVGKEKIIK+GMM
Sbjct: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMM 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMK VKHPNIVELHEVMASKSKIYFAME VRGGELFSKI KGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFSKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLD+DGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEV+GK GYDGAKADLWSCGVILYVLLAG+LPFQDDNMVAMYRKIYRGDF+CP W
Sbjct: 181 PAYVAPEVIGKHGYDGAKADLWSCGVILYVLLAGFLPFQDDNMVAMYRKIYRGDFKCPSW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITK-EHEEMEENLAKSNQPA 300
           FSPEARRL+TKLLDPNPVTR GI KITSS WFKKSVPKS+ITK E E+ EEN  KS  PA
Sbjct: 241 FSPEARRLITKLLDPNPVTRAGITKITSSSWFKKSVPKSLITKDEQEQTEENFTKSKPPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRK 360
           ETLNAFHIISLSEGFDLSPLFEEKKREEKEE+RF TSS ASSVISKLEEV KAVKFDV+K
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSTASSVISKLEEVGKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKD 420
           SE+WVRLQGQE GRKGKL IA DIFAMTPSFVVVEVKKDNGDTLEYNQFCR+ELRPALKD
Sbjct: 361 SETWVRLQGQEMGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGSSTPA 433
           IVWTSPTG+STPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of CmoCh05G012620 vs. NCBI nr
Match: XP_022946664.1 (CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita moschata])

HSP 1 Score: 864.0 bits (2231), Expect = 5.5e-247
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM
Sbjct: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY
Sbjct: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW
Sbjct: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE 300
           FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE
Sbjct: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE 300

Query: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS 360
           TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS
Sbjct: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS 360

Query: 361 ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420
           ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI
Sbjct: 361 ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420

Query: 421 VWTSPTGSSTPA 433
           VWTSPTGSSTPA
Sbjct: 421 VWTSPTGSSTPA 432

BLAST of CmoCh05G012620 vs. NCBI nr
Match: KAG6599345.1 (CBL-interacting serine/threonine-protein kinase 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030335.1 CBL-interacting serine/threonine-protein kinase 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 858.2 bits (2216), Expect = 3.0e-245
Identity = 429/432 (99.31%), Postives = 430/432 (99.54%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM
Sbjct: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY
Sbjct: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW
Sbjct: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE 300
           FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKE EEMEENLAKSNQPAE
Sbjct: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEQEEMEENLAKSNQPAE 300

Query: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS 360
           TLNAFHIISLSEGFDLSPLFEEKKREEKEE+RFLTSSPASSVISKLEEVAKAVKFDVRKS
Sbjct: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFLTSSPASSVISKLEEVAKAVKFDVRKS 360

Query: 361 ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420
           ESWVRLQGQEKGRKGKLAIA DIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI
Sbjct: 361 ESWVRLQGQEKGRKGKLAIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420

Query: 421 VWTSPTGSSTPA 433
           VWTSPTGSSTPA
Sbjct: 421 VWTSPTGSSTPA 432

BLAST of CmoCh05G012620 vs. NCBI nr
Match: XP_022999362.1 (CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita maxima])

HSP 1 Score: 852.8 bits (2202), Expect = 1.3e-243
Identity = 426/432 (98.61%), Postives = 428/432 (99.07%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM
Sbjct: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMK VKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW
Sbjct: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE 300
           FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPK+VITKE EEMEENLAKSNQPAE
Sbjct: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKTVITKEQEEMEENLAKSNQPAE 300

Query: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS 360
           TLNAFHIISLSEGFDLSPLFEEKKREEKEE+RF TSSPASSVISKLEEVAKAVKFDVRKS
Sbjct: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVAKAVKFDVRKS 360

Query: 361 ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420
           ESWVRLQGQEKGRKGKLAIA DIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI
Sbjct: 361 ESWVRLQGQEKGRKGKLAIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420

Query: 421 VWTSPTGSSTPA 433
           VWTSPTGSSTPA
Sbjct: 421 VWTSPTGSSTPA 432

BLAST of CmoCh05G012620 vs. NCBI nr
Match: XP_023546991.1 (CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 849.4 bits (2193), Expect = 1.4e-242
Identity = 424/432 (98.15%), Postives = 428/432 (99.07%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGK+VAMKVVGKEKIIKIGMM
Sbjct: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKNVAMKVVGKEKIIKIGMM 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMK VKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEV+GKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE 300
           FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKS+PKSVITKE EEMEENLAKSNQPAE
Sbjct: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSIPKSVITKEQEEMEENLAKSNQPAE 300

Query: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRKS 360
           TLNAFHIISLSEGFDLSPLFEEKKREEKEE+RFLTSSPASSVISKLEEVAKAVKFDVRKS
Sbjct: 301 TLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFLTSSPASSVISKLEEVAKAVKFDVRKS 360

Query: 361 ESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420
           ESWVRLQGQEKGRKGKLAIA DIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI
Sbjct: 361 ESWVRLQGQEKGRKGKLAIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDI 420

Query: 421 VWTSPTGSSTPA 433
           VWTSPTGSST A
Sbjct: 421 VWTSPTGSSTRA 432

BLAST of CmoCh05G012620 vs. NCBI nr
Match: XP_022965758.1 (CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita maxima])

HSP 1 Score: 802.4 bits (2071), Expect = 2.0e-228
Identity = 396/433 (91.45%), Postives = 417/433 (96.30%), Query Frame = 0

Query: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60
           MA+KGRDG +SSLLDGKYE GRLLGHGTFAKVYHARNL+TGK+VAMKVVGKEKIIK+GM 
Sbjct: 1   MADKGRDGGYSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKTVAMKVVGKEKIIKVGMT 60

Query: 61  EQIKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120
           EQIKREISVMK VKHPNIVELHEVMASKSKIYFAME VRGGELF+KI KGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFTKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLD+DGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240
           PAYVAPEV+GKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMY+KIYRGDF+CPPW
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYKKIYRGDFKCPPW 240

Query: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKSVITKEHEE-MEENLAKSNQPA 300
           FS EARRLVTKLLDPNPVTR+GI KITSS WFKKSVPK++ITKE +E +EEN  KS QPA
Sbjct: 241 FSSEARRLVTKLLDPNPVTRIGITKITSSSWFKKSVPKAIITKEEQERIEENFTKSKQPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAKAVKFDVRK 360
           ETLNAFHIISLSEGFDLSPLFEEKKRE+KEE+RF TSSPASSVISKLEEVAKAVKFDV+K
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREDKEEMRFATSSPASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKD 420
           SESWVRLQGQEKGRKGKLAIA DIFA+TPSFVVVEVKKDNGDTLEYNQFCR+ELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLAIAADIFALTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGSSTPA 433
           IVWTSPTG+STPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of CmoCh05G012620 vs. TAIR 10
Match: AT4G30960.1 (SOS3-interacting protein 3 )

HSP 1 Score: 662.9 bits (1709), Expect = 1.7e-190
Identity = 328/430 (76.28%), Postives = 370/430 (86.05%), Query Frame = 0

Query: 3   NKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQ 62
           N    G  + LL G+YE GRLLGHGTFAKVYHARN+QTGKSVAMKVVGKEK++K+GM++Q
Sbjct: 9   NGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQ 68

Query: 63  IKREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQ 122
           IKREISVM+ VKHPNIVELHEVMASKSKIYFAMELVRGGELF+K+ KGRLREDVARVYFQ
Sbjct: 69  IKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQ 128

Query: 123 QLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPA 182
           QLISA+DFCHSRGVYHRDLKPENLLLD++GNLKVTDFGL+AF+EHLKQDGLLHTTCGTPA
Sbjct: 129 QLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPA 188

Query: 183 YVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFS 242
           YVAPEV+ K+GYDGAKADLWSCGVIL+VLLAGYLPFQDDN+V MYRKIYRGDF+CP W S
Sbjct: 189 YVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLS 248

Query: 243 PEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKS-------VITKEHEEMEENLAKS 302
            +ARRLVTKLLDPNP TR+ I K+  S WFKK   +S        IT   E+++  + KS
Sbjct: 249 SDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKS 308

Query: 303 NQPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVAK-AVK 362
            +  ETLNAFHII+LSEGFDLSPLFEEKK+EEK E+RF TS PASSVIS LEE A+   K
Sbjct: 309 KEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNK 368

Query: 363 FDVRKSESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELR 422
           FDVRKSES VR++G++ GRKGKLA+  +IFA+ PSFVVVEVKKD+GDTLEYN FC   LR
Sbjct: 369 FDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALR 428

Query: 423 PALKDIVWTS 425
           PALKDI WTS
Sbjct: 429 PALKDIFWTS 438

BLAST of CmoCh05G012620 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 513.8 bits (1322), Expect = 1.3e-145
Identity = 250/414 (60.39%), Postives = 327/414 (78.99%), Query Frame = 0

Query: 17  KYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQIKREISVMKRVKHP 76
           KYE GRLLG GTFAKVYHARN++TG+SVA+KV+ K+K+ K+G+++QIKREISVM+ V+HP
Sbjct: 11  KYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRLVRHP 70

Query: 77  NIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQQLISAIDFCHSRGV 136
           ++V LHEVMASK+KIYFAME V+GGELF K+ KG+L+E++AR YFQQLI AID+CHSRGV
Sbjct: 71  HVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIGAIDYCHSRGV 130

Query: 137 YHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVMGKQGYDG 196
           YHRDLKPENLLLD++G+LK++DFGL+A  E  +QDGLLHTTCGTPAYVAPEV+GK+GYDG
Sbjct: 131 YHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGKKGYDG 190

Query: 197 AKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFSPEARRLVTKLLDPN 256
           AKAD+WSCGV+LYVLLAG+LPF + N+V MYRKI +G+F+CP WF PE ++L++++LDPN
Sbjct: 191 AKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSRILDPN 250

Query: 257 PVTRVGIAKITSSLWFKKSVPKSVITKEHEEMEENLAKSNQPAE------TLNAFHII-S 316
           P +R+ I KI  + WF+K   K    K  E  + +   S+  A       + NAF +I S
Sbjct: 251 PNSRIKIEKIMENSWFQKGFKKIETPKSPESHQIDSLISDVHAAFSVKPMSYNAFDLISS 310

Query: 317 LSEGFDLSPLFEEKKREEKEEIRFLTSSPASSVISKLEEVA-KAVKFDVRKSESWVRLQG 376
           LS+GFDLS LFE   +EE+ E +F T   A  ++SK EE+A  + +F++ KS+  V+++ 
Sbjct: 311 LSQGFDLSGLFE---KEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVGVKMED 370

Query: 377 QEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKDIVW 423
           + +GRKG LAI V+IF +T SF +VE KK  GDT+EY QFC  ELRP+LKDIVW
Sbjct: 371 KREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVW 421

BLAST of CmoCh05G012620 vs. TAIR 10
Match: AT4G18700.1 (CBL-interacting protein kinase 12 )

HSP 1 Score: 495.0 bits (1273), Expect = 6.3e-140
Identity = 246/440 (55.91%), Postives = 321/440 (72.95%), Query Frame = 0

Query: 12  SLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQIKREISVMK 71
           +L+ G+YE G+LLGHGTFAKVY ARN++T +SVA+KV+ KEK++K G++  IKREIS+++
Sbjct: 20  ALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVLKGGLIAHIKREISILR 79

Query: 72  RVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQQLISAIDFC 131
           RV+HPNIV+L EVMA+K+KIYF ME VRGGELF+K+ KGRL+E+VAR YFQQLISA+ FC
Sbjct: 80  RVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEEVARKYFQQLISAVTFC 139

Query: 132 HSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVMGK 191
           H+RGVYHRDLKPENLLLD++GNLKV+DFGL+A S+ ++QDGL HT CGTPAYVAPEV+ +
Sbjct: 140 HARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLAR 199

Query: 192 QGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFSPEARRLVTK 251
           +GYD AK D+WSCGVIL+VL+AGYLPF D N++AMY+KIYRG+FRCP WFS E  RL++K
Sbjct: 200 KGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTELTRLLSK 259

Query: 252 LLDPNPVTRVGIAKITSSLWFKKSV---------PKSVITKEHEEMEENLAKSNQPA--- 311
           LL+ NP  R    +I  + WFKK            K     + +E+E +  +S++ +   
Sbjct: 260 LLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVVDDDELESDSVESDRDSAAS 319

Query: 312 -------------------ETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPAS 371
                               +LNAF IIS S+GFDLS LF+    ++ E  RF++ +P S
Sbjct: 320 ESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFD----DDGEGSRFVSGAPVS 379

Query: 372 SVISKLEEVAKAVKFDVRKSESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNG 421
            +ISKLEE+AK V F VRK +  V L+G  +G KG L IA +IF +TPS VVVEVKK  G
Sbjct: 380 KIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFELTPSLVVVEVKKKGG 439

BLAST of CmoCh05G012620 vs. TAIR 10
Match: AT1G29230.1 (CBL-interacting protein kinase 18 )

HSP 1 Score: 491.5 bits (1264), Expect = 6.9e-139
Identity = 247/440 (56.14%), Postives = 317/440 (72.05%), Query Frame = 0

Query: 11  SSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQIKREISVM 70
           +++L GKYE G+LLGHGTFAKVY A+N+++G  VA+KV+ KEKI+K G++  IKREIS++
Sbjct: 67  NNILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISIL 126

Query: 71  KRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQQLISAIDF 130
           +RV+HP IV L EVMA+KSKIYF ME V GGELF+ + KGRL E+ AR YFQQLIS++ F
Sbjct: 127 RRVRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSF 186

Query: 131 CHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVMG 190
           CH RGVYHRDLKPENLLLD  GNLKV+DFGL+A +E L+QDGL HT CGTPAY+APEV+ 
Sbjct: 187 CHGRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLT 246

Query: 191 KQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFSPEARRLVT 250
           ++GYD AKAD+WSCGVIL+VL+AG++PF D N++ MY+KIY+G+FRCP WFS +  RL+T
Sbjct: 247 RKGYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLT 306

Query: 251 KLLDPNPVTRVGIAKITSSLWFKKS-------VPKSVITKEHEEMEENLAKSNQPA---- 310
           +LLD NP TR+ I +I  + WFKK        +    + +E E+ EE  + S + +    
Sbjct: 307 RLLDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSE 366

Query: 311 ------------------ETLNAFHIISLSEGFDLSPLFEEKKREEKEEIRFLTSSPASS 370
                              +LNAF IIS S GFDLS LFEE   E  E  RF++ +P S 
Sbjct: 367 SDAEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEE---EGGEGTRFVSGAPVSK 426

Query: 371 VISKLEEVAKAVKFDVRKSESWVRLQGQEKGRKGKLAIAVDIFAMTPSFVVVEVKKDNGD 422
           +ISKLEE+AK V F VRK E  +RL+G  +G KG L IA +IF +TPS VVVEVKK  GD
Sbjct: 427 IISKLEEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGD 486

BLAST of CmoCh05G012620 vs. TAIR 10
Match: AT5G45810.1 (CBL-interacting protein kinase 19 )

HSP 1 Score: 489.2 bits (1258), Expect = 3.4e-138
Identity = 246/451 (54.55%), Postives = 323/451 (71.62%), Query Frame = 0

Query: 4   KGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMMEQI 63
           K     H +L+ GKYE GRLLGHGTFAKVY ARN Q+G+SVA+KV+ KEK++K G++  I
Sbjct: 14  KQDQSNHQALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIAHI 73

Query: 64  KREISVMKRVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVYFQQ 123
           KREIS+++RV+HPNIV+L EVMA+KSKIYF ME V+GGELF+K+ KGRL+E++AR YFQQ
Sbjct: 74  KREISILRRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYFQQ 133

Query: 124 LISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAY 183
           LISA+ FCH RGVYHRDLKPENLLLD++GNLKV+DFGL+A S+ ++QDGL HT CGTPAY
Sbjct: 134 LISAVSFCHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAY 193

Query: 184 VAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPWFSP 243
           VAPEV+ ++GYDGAK D+WSCGVIL+VL+AG+LPF D N++AMY+KIYRGDFRCP WF  
Sbjct: 194 VAPEVLARKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWFPV 253

Query: 244 EARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVP--KSVITKEH-----------EEMEE 303
           E  RL+ ++L+  P  R  +  I  + WFKK     K  +  +H           E +E 
Sbjct: 254 EINRLLIRMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESIES 313

Query: 304 NLAKSNQPAE--------------------TLNAFHIISLSEGFDLSPLFEEKKREEKEE 363
              +S+  +E                    +LNAF +IS S GFDLS LFE    ++ E 
Sbjct: 314 VSGRSSTVSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFE----DDGEG 373

Query: 364 IRFLTSSPASSVISKLEEVAKAVKFDVRKSESWVRLQGQEKG-RKGKLAIAVDIFAMTPS 421
            RF++ +P   +ISKLEE+A+ V F VRK +  V L+G  +G  KG L+IA +IF +TP+
Sbjct: 374 SRFVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPA 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O655542.4e-18976.28CBL-interacting serine/threonine-protein kinase 6 OS=Arabidopsis thaliana OX=370... [more]
Q9LWM45.7e-15461.02CBL-interacting protein kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q6Z9F49.4e-14961.42CBL-interacting protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q5W7367.9e-14859.27CBL-interacting protein kinase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q9FJ541.8e-14460.39CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A6J1G4G92.7e-247100.00Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KAP06.1e-24498.61Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1HPX19.5e-22991.45Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FDM51.6e-22891.45Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0LL731.2e-22691.22Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_2... [more]
Match NameE-valueIdentityDescription
XP_022946664.15.5e-247100.00CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita moschata][more]
KAG6599345.13.0e-24599.31CBL-interacting serine/threonine-protein kinase 6, partial [Cucurbita argyrosper... [more]
XP_022999362.11.3e-24398.61CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita maxima][more]
XP_023546991.11.4e-24298.15CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita pepo subsp. pe... [more]
XP_022965758.12.0e-22891.45CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G30960.11.7e-19076.28SOS3-interacting protein 3 [more]
AT5G45820.11.3e-14560.39CBL-interacting protein kinase 20 [more]
AT4G18700.16.3e-14055.91CBL-interacting protein kinase 12 [more]
AT1G29230.16.9e-13956.14CBL-interacting protein kinase 18 [more]
AT5G45810.13.4e-13854.55CBL-interacting protein kinase 19 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 18..272
e-value: 7.9E-101
score: 351.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 18..272
e-value: 6.2E-71
score: 238.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 18..272
score: 50.548145
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 100..299
e-value: 4.3E-57
score: 194.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 15..99
e-value: 2.8E-28
score: 99.9
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 300..423
e-value: 8.6E-47
score: 160.3
NoneNo IPR availablePIRSRPIRSR037568-1PIRSR037568-1coord: 17..272
e-value: 4.6E-40
score: 134.8
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 17..211
e-value: 3.1E-11
score: 40.7
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 22..221
e-value: 3.6E-9
score: 34.1
NoneNo IPR availablePIRSRPIRSR000550-2PIRSR000550-2coord: 16..266
e-value: 1.8E-51
score: 173.2
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 17..265
e-value: 2.9E-56
score: 189.0
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 10..425
NoneNo IPR availablePANTHERPTHR43895:SF53NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 10..425
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 303..418
e-value: 1.35551E-46
score: 154.652
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 17..271
e-value: 8.93731E-170
score: 474.2
IPR004041NAF domainPFAMPF03822NAFcoord: 300..359
e-value: 1.8E-22
score: 79.2
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 136..148
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 24..51
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 297..321
score: 12.275091
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 15..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G012620.1CmoCh05G012620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity