CmoCh05G012420 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh05G012420
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Ribonuclease T(2)
Location	Cmo_Chr05: 9669277 .. 9669997 (+)
RNA-Seq Expression	CmoCh05G012420
Synteny	CmoCh05G012420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTATGATTATGTTGTTGTTGCTGTTCAACTTGATGCTAACGGCAAGCTATGAGTTCGAATACTTTCAAATGGTCCAACAATGGGCACCAACTTTTTGCAGTGGGATTGGTGTTACTTGTAAAGTTCAACCACCTCAAAACAAGTTTACTATTCATGGGTTATGGCCAAGCAACTTCTCGAATGCAAAGCTCATCTGTTCGGGAGGAGATTTACAACAAAATAAGGTTTTTATCCTCTTTAATATATATATTTTTTTCATAATTAGTTCAAATATTCGTGAAAAATAATGGTAGTTTAAAAATGTAGCTTCCTCATCGACTTTCAACCAAACTTAAAAAACATTGGCCAAACTTGAAATGTGGTAATGACTTCGATTTTTGGAGATACGAATGGAGAAAACATGGTAGGTGTTCAAATCCCCCGCTCAACCCTAATCAATATTTACATACAACTATACGCATGTACTTGAATTATGATCTCCTGGGCATGTTGAATGCGGCTGGACTCAGTCCGAGCACAACGTTCCGTCAATATACTTACATGGAAAACGTACTTAAGAATGCAATAGGAGCGGTCCCAAGCCTTCGTTGCAATGTCAACAGCCAATCAAATGAGGACCAATTGTATGAAATTGTTATTTGTTTTGAAGAAAATAGTACAAATTTGATAGATTGTACATATGCCCCAGGAAATTGTCCAGATTGGTTTGTTTGGTAG

mRNA sequence

ATGTCTATGATTATGTTGTTGTTGCTGTTCAACTTGATGCTAACGGCAAGCTATGAGTTCGAATACTTTCAAATGGTCCAACAATGGGCACCAACTTTTTGCAGTGGGATTGGTGTTACTTGTAAAGTTCAACCACCTCAAAACAAGTTTACTATTCATGGGTTATGGCCAAGCAACTTCTCGAATGCAAAGCTCATCTGTTCGGGAGGAGATTTACAACAAAATAAGCTTCCTCATCGACTTTCAACCAAACTTAAAAAACATTGGCCAAACTTGAAATGTGGTAATGACTTCGATTTTTGGAGATACGAATGGAGAAAACATGGTAGGTGTTCAAATCCCCCGCTCAACCCTAATCAATATTTACATACAACTATACGCATGTACTTGAATTATGATCTCCTGGGCATGTTGAATGCGGCTGGACTCAGTCCGAGCACAACGTTCCGTCAATATACTTACATGGAAAACGTACTTAAGAATGCAATAGGAGCGGTCCCAAGCCTTCGTTGCAATGTCAACAGCCAATCAAATGAGGACCAATTGTATGAAATTGTTATTTGTTTTGAAGAAAATAGTACAAATTTGATAGATTGTACATATGCCCCAGGAAATTGTCCAGATTGGTTTGTTTGGTAG

Coding sequence (CDS)

ATGTCTATGATTATGTTGTTGTTGCTGTTCAACTTGATGCTAACGGCAAGCTATGAGTTCGAATACTTTCAAATGGTCCAACAATGGGCACCAACTTTTTGCAGTGGGATTGGTGTTACTTGTAAAGTTCAACCACCTCAAAACAAGTTTACTATTCATGGGTTATGGCCAAGCAACTTCTCGAATGCAAAGCTCATCTGTTCGGGAGGAGATTTACAACAAAATAAGCTTCCTCATCGACTTTCAACCAAACTTAAAAAACATTGGCCAAACTTGAAATGTGGTAATGACTTCGATTTTTGGAGATACGAATGGAGAAAACATGGTAGGTGTTCAAATCCCCCGCTCAACCCTAATCAATATTTACATACAACTATACGCATGTACTTGAATTATGATCTCCTGGGCATGTTGAATGCGGCTGGACTCAGTCCGAGCACAACGTTCCGTCAATATACTTACATGGAAAACGTACTTAAGAATGCAATAGGAGCGGTCCCAAGCCTTCGTTGCAATGTCAACAGCCAATCAAATGAGGACCAATTGTATGAAATTGTTATTTGTTTTGAAGAAAATAGTACAAATTTGATAGATTGTACATATGCCCCAGGAAATTGTCCAGATTGGTTTGTTTGGTAG

Protein sequence

MSMIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNEDQLYEIVICFEENSTNLIDCTYAPGNCPDWFVW

Homology

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BLAST of CmoCh05G012420 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.5e-27
Identity = 62/182 (34.07%), Postives = 84/182 (46.15%), Query Frame = 0


Query: 20  FEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSNAKLIC-SGGDLQQNKLP 79
           F+ F  VQQW P  CS              FTIHGLWP     +   C  G      K+ 
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 80  HRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYLHTTIRMYLNYDLLGML 139
           H L ++L   WPN+   N+  FW +EW KHG CS    N   Y    + M  NYD++G L
Sbjct: 61  H-LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 140 NAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNEDQLYEIVICFEENSTNLID 199
                 P+   +    ++  LK   G  P LRC  + Q+    L ++V CF ++ + LID
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLID 180

Query: 200 CT 201
           CT
Sbjct: 181 CT 181

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BLAST of CmoCh05G012420 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.2e-25
Identity = 67/216 (31.02%), Postives = 106/216 (49.07%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTA---SYEFEYFQMVQQWAPTFCSGIGVTC--KVQPPQNKFTIHGL 60
           + +I LL++   + +A   S +F++F  VQQW  ++C      C      P   F IHGL
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGL 67

Query: 61  WPSNFSNAKL--ICSGGDLQQNKLPHRLSTKLKKHWPNLKC--GNDFDFWRYEWRKHGRC 120
           WP N+ +      C       +     L T +KK WP L C  G+   FW +EW KHG C
Sbjct: 68  WP-NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 127

Query: 121 SNPPLNPNQYLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRC 180
           S   ++ ++Y  T + +    +LLG L  AG++P         + + +K +IG  P + C
Sbjct: 128 SESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVEC 187

Query: 181 NVNSQSNEDQLYEIVICFEENSTNLIDCTYAP-GNC 207
           N +   N  QLY++ +C + + + LI+C   P G C
Sbjct: 188 NRDGSGN-SQLYQVYLCVDRSGSGLIECPVFPHGKC 221

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BLAST of CmoCh05G012420 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.5e-24
Identity = 64/214 (29.91%), Postives = 102/214 (47.66%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTASY--EFEYFQMVQQWAPTFCSGIGVTCKVQ--PPQNKFTIHGLW 60
           M   + +L    +   S+  +F++F  V QW   +C      C  Q   P   F IHGLW
Sbjct: 1   MKFFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLW 60

Query: 61  PSNFSNA-KLICSGGDLQQNKLPHRLSTKLKKHWPNLKC-GND-FDFWRYEWRKHGRCSN 120
           P+  +      C+      +     L + L++ WP L C  ND   FW +EW KHG C+ 
Sbjct: 61  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 120

Query: 121 PPLNPNQYLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNV 180
             L+ + Y    +++    +LL  L  AG+ P   F +   +EN +K  +G  P + CN 
Sbjct: 121 SELDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNK 180

Query: 181 NSQSNEDQLYEIVICFEENSTNLIDCTYAP-GNC 207
           +S S+  QLY+I +C + +++  I+C   P G C
Sbjct: 181 DS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRC 213

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BLAST of CmoCh05G012420 vs. ExPASy Swiss-Prot
Match: P83618 (Ribonuclease-like storage protein OS=Panax ginseng OX=4054 PE=1 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 5.6e-22
Identity = 55/186 (29.57%), Postives = 90/186 (48.39%), Query Frame = 0


Query: 16  ASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSNA-KLICSGGDLQQ 75
           + Y +  F +  QW   FC  +   C  +   N FTIHGL+P N      L C G     
Sbjct: 25  SDYPWAMFALRLQWPAGFCE-VNNACDTKSLLNTFTIHGLYPYNAKGTPALYCDGTAFDV 84

Query: 76  NKLPHRLSTKLKKHWPNLKCG-NDFDFWRYEWRKHGRCSNPPLNPNQYLHTTIRMYLNYD 135
           N +   L+ ++   WP+ +    D  FW +EW+KHGRCS   L    Y  T +     +D
Sbjct: 85  NSVSDFLA-EMHLAWPSHETNTEDIQFWEHEWKKHGRCSEALLKQTDYFRTALAFRKAFD 144

Query: 136 LLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNEDQLYEIVICFEENS 195
           ++G+LN  G+ P+    +   ++  +K  + AVP +    N +++E  L +I +C  + +
Sbjct: 145 IVGLLNQEGIYPNNDLYRPKMIKEAIKKHLNAVPEIDFTKN-ENSEYVLTDINVCVNQQA 204

Query: 196 TNLIDC 200
           T  +DC
Sbjct: 205 TRFVDC 207

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BLAST of CmoCh05G012420 vs. ExPASy Swiss-Prot
Match: Q40381 (Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-21
Identity = 70/214 (32.71%), Postives = 101/214 (47.20%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTASY-EFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSN 60
           ++ ++ +LLF  +L+  Y  FEY Q+V QW   FC      CK  P  N FTIHGLWP N
Sbjct: 6   LTSVLFVLLF--VLSPIYGAFEYMQLVLQWPTAFCH--TTPCKRIP--NNFTIHGLWPDN 65

Query: 61  FSNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLK-----CGNDFDFWRYEWRKHGRCSNP 120
            S     C+  +  +N         L K WP+L      C    +FWR+E+ KHG+C + 
Sbjct: 66  VSTTLNYCAAKENFKNIEDDTKKDDLYKRWPDLTTAETYCKQHQNFWRHEYNKHGKCCSE 125

Query: 121 PLNPNQYLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTY--MENVLKNAIGAVPSLRCN 180
             N  QY    + +   +DLL  L   G+ P    + YT   + + +K      P+L C 
Sbjct: 126 SYNREQYFDLAMALKDKFDLLSSLRNHGIIPGRGMK-YTVQKINSTIKKITQGYPNLSCT 185

Query: 181 VNSQSNEDQLYEIVICFEENSTNLIDCTYAPGNC 207
                   +L EI ICF+    N+I+C + P  C
Sbjct: 186 KGIM----ELVEIGICFDSMVKNVINCPH-PKTC 207

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BLAST of CmoCh05G012420 vs. ExPASy TrEMBL
Match: A0A6J1G4E7 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111450689 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 2.6e-126
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNF 60
           MSMIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNF
Sbjct: 1   MSMIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNF 60

Query: 61  SNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ 120
           SNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ
Sbjct: 61  SNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ 120

Query: 121 YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED 180
           YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED
Sbjct: 121 YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED 180

Query: 181 QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW 213
           QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW
Sbjct: 181 QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW 212

[Close]

BLAST of CmoCh05G012420 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 4.3e-49
Identity = 100/206 (48.54%), Postives = 134/206 (65.05%), Query Frame = 0


Query: 12  LMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSNAKLICSGGD 71
           L+L  + +F+YFQMVQQW P  CS + V C VQPP   FTIHGLWPSN+SNA+L+C+   
Sbjct: 18  LILCVNAQFDYFQMVQQWPPAKCSSVKVRCHVQPP-TMFTIHGLWPSNYSNARLVCAPQP 77

Query: 72  LQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYLHTTIRMYLN 131
               ++   L  +L  +WP++  G +  FW++EW +HG CSNPP N  QY   T+ +  N
Sbjct: 78  FNPTQIA-ALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYFQITLNIRTN 137

Query: 132 --YDLLGMLNAAGLSPS-TTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNE-DQLYEIVI 191
             YDLL +LNAAGL PS T  R+Y  +E  ++ A G  P LRCN+N+QS E  QL EI++
Sbjct: 138 HSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQLLEIIL 197

Query: 192 CFEENSTNLIDCT-YAPGNCPDWFVW 213
           CF+++   LIDCT +A   CP  FVW
Sbjct: 198 CFDKDGITLIDCTEFAGITCPPQFVW 221

[Close]

BLAST of CmoCh05G012420 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 8.1e-48
Identity = 102/214 (47.66%), Postives = 133/214 (62.15%), Query Frame = 0


Query: 6   LLLLFNLMLTAS---YEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSN 65
           ++ +F  +L  S    +F+YFQMVQQW P  CS  GV C   PP   FTIHGLWPSN+SN
Sbjct: 7   IVSIFKFVLLTSCVKAQFDYFQMVQQWPPATCS--GVRCYANPPA-MFTIHGLWPSNYSN 66

Query: 66  AKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYL 125
             L+C+G      ++   L  +L  +WP++  GN+  FW++EW  HG CS+PP N  QY 
Sbjct: 67  VPLVCAGQPFNPAQIA-TLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYF 126

Query: 126 HTT--IRMYLNYDLLGMLNAAGLSP-STTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNE 185
            TT  IR   NYDLL +LN AGL P +T  RQY  +E  ++ A G  P LRCN N+QS +
Sbjct: 127 QTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNKNAQSKK 186

Query: 186 DQLYEIVICFEENSTNLIDCTYAPG-NCPDWFVW 213
            QL+EI++CF++N   LIDCT   G  CP  FVW
Sbjct: 187 KQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVW 216

[Close]

BLAST of CmoCh05G012420 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 3.1e-47
Identity = 101/214 (47.20%), Postives = 133/214 (62.15%), Query Frame = 0


Query: 6   LLLLFNLMLT---ASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSN 65
           ++ +FNL+L       +F+YFQMVQQW P  CS  GV C   PP   FTIHGLWPS +SN
Sbjct: 8   IVSIFNLVLLTIGVKAQFDYFQMVQQWPPATCS--GVRCYANPPA-MFTIHGLWPSTYSN 67

Query: 66  AKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYL 125
              +C+G    + ++   L  +L  +WP++  GN+  FW++EW  HG CS+PP N  QY 
Sbjct: 68  VPPVCAGQPFDRAQIA-TLEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQYF 127

Query: 126 HTT--IRMYLNYDLLGMLNAAGLSP-STTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNE 185
            TT  IR   NYDLL +LN AGL P +TT RQY  +E  ++ A G  P LRCN N+QS  
Sbjct: 128 QTTLNIRTNHNYDLLAILNTAGLGPTATTTRQYQAIEGAIQAATGKKPGLRCNKNAQSKR 187

Query: 186 DQLYEIVICFEENSTNLIDCTYAPG-NCPDWFVW 213
            QL+EI++CF++N   LI+CT   G  CP  FVW
Sbjct: 188 KQLFEIILCFDKNGVTLINCTQFAGITCPSQFVW 217

[Close]

BLAST of CmoCh05G012420 vs. ExPASy TrEMBL
Match: A0A6J1HV84 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111466502 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 4.6e-43
Identity = 94/211 (44.55%), Postives = 124/211 (58.77%), Query Frame = 0


Query: 7   LLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSNAKLI 66
           L L  L+ + + +FE FQ+VQQW P  C+   V C   P   +FTIHGLWP+N++   L+
Sbjct: 13  LSLSTLIFSVNAQFELFQLVQQWPPATCT--NVHCGQLPTPTRFTIHGLWPANYTKPYLV 72

Query: 67  CSGGDLQQNKLP--HRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYLHT 126
           CSGG      +     L   L  +WPNLK G+   FWR EW KHG CS    N  QY  T
Sbjct: 73  CSGGTKFSETIQGFSALKPALVYYWPNLKKGSHKSFWRKEWDKHGACSENVFNQVQYFQT 132

Query: 127 TIRMYLN--YDLLGMLNAAGLSPS-TTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNEDQ 186
            + +  N  YDLL +LNAA L P+   FRQY  +EN +K A G  P LRCN N  + + Q
Sbjct: 133 ALNIRTNPKYDLLAILNAANLGPTGNIFRQYHVIENAIKAATGKKPGLRCNTNQNTKQTQ 192

Query: 187 LYEIVICFEENSTNLIDCTYAPGNCPDWFVW 213
           L+EIV+CF+++   +IDC +A  NCP  FV+
Sbjct: 193 LHEIVLCFDKDGATIIDCPFA--NCPKQFVF 219

[Close]

BLAST of CmoCh05G012420 vs. NCBI nr
Match: XP_022946697.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 461.1 bits (1185), Expect = 5.3e-126
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNF 60
           MSMIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNF
Sbjct: 1   MSMIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNF 60

Query: 61  SNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ 120
           SNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ
Sbjct: 61  SNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ 120

Query: 121 YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED 180
           YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED
Sbjct: 121 YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED 180

Query: 181 QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW 213
           QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW
Sbjct: 181 QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW 212

[Close]

BLAST of CmoCh05G012420 vs. NCBI nr
Match: XP_023547237.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 456.8 bits (1174), Expect = 9.9e-125
Identity = 210/212 (99.06%), Postives = 211/212 (99.53%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNF 60
           MSMIMLLLLFNLMLTASYEFEYFQMVQQW PTFCSGIGVTCKVQPPQNKFTIHGLWPSNF
Sbjct: 1   MSMIMLLLLFNLMLTASYEFEYFQMVQQWPPTFCSGIGVTCKVQPPQNKFTIHGLWPSNF 60

Query: 61  SNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ 120
           SNAKLICSGGDLQQNKLPHRLSTKLKK+WPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ
Sbjct: 61  SNAKLICSGGDLQQNKLPHRLSTKLKKYWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQ 120

Query: 121 YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED 180
           YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED
Sbjct: 121 YLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNED 180

Query: 181 QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW 213
           QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW
Sbjct: 181 QLYEIVICFEENSTNLIDCTYAPGNCPDWFVW 212

[Close]

BLAST of CmoCh05G012420 vs. NCBI nr
Match: XP_038906187.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 217.6 bits (553), Expect = 1.0e-52
Identity = 104/214 (48.60%), Postives = 142/214 (66.36%), Query Frame = 0


Query: 3   MIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSN 62
           +I +  L  L+ +   +++YFQMVQQW P  CSG+GV C+ QPP + FTIHGLWPSN+S 
Sbjct: 7   VISIFSLALLVFSVKAQYDYFQMVQQWPPATCSGVGVRCRAQPP-SMFTIHGLWPSNYST 66

Query: 63  AKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYL 122
           A L+C+G      ++   +  +L  +WP++  GN+  FW++EW  HG CS+PP N  QY 
Sbjct: 67  AHLVCTGLPFDPTQIA-AIRPQLNTYWPDVIRGNNQRFWQHEWDAHGTCSDPPFNQRQYF 126

Query: 123 HTT--IRMYLNYDLLGMLNAAGLSPSTTF-RQYTYMENVLKNAIGAVPSLRCNVNSQSNE 182
            TT  IRM  NYDLL +LNAAGL P+ T  RQY  +EN ++ A G +P LRCN N+Q+  
Sbjct: 127 QTTLNIRMNHNYDLLAILNAAGLGPTATMVRQYQAIENAIQAATGKIPGLRCNTNAQTGS 186

Query: 183 DQLYEIVICFEENSTNLIDCT-YAPGNCPDWFVW 213
            QL+EI++CF++N   LIDCT +A   CP  FVW
Sbjct: 187 RQLFEIILCFDKNGVTLIDCTPFAGITCPLQFVW 218

[Close]

BLAST of CmoCh05G012420 vs. NCBI nr
Match: XP_022934593.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 204.5 bits (519), Expect = 8.9e-49
Identity = 100/206 (48.54%), Postives = 134/206 (65.05%), Query Frame = 0


Query: 12  LMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSNAKLICSGGD 71
           L+L  + +F+YFQMVQQW P  CS + V C VQPP   FTIHGLWPSN+SNA+L+C+   
Sbjct: 18  LILCVNAQFDYFQMVQQWPPAKCSSVKVRCHVQPP-TMFTIHGLWPSNYSNARLVCAPQP 77

Query: 72  LQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYLHTTIRMYLN 131
               ++   L  +L  +WP++  G +  FW++EW +HG CSNPP N  QY   T+ +  N
Sbjct: 78  FNPTQIA-ALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYFQITLNIRTN 137

Query: 132 --YDLLGMLNAAGLSPS-TTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNE-DQLYEIVI 191
             YDLL +LNAAGL PS T  R+Y  +E  ++ A G  P LRCN+N+QS E  QL EI++
Sbjct: 138 HSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQLLEIIL 197

Query: 192 CFEENSTNLIDCT-YAPGNCPDWFVW 213
           CF+++   LIDCT +A   CP  FVW
Sbjct: 198 CFDKDGITLIDCTEFAGITCPPQFVW 221

[Close]

BLAST of CmoCh05G012420 vs. NCBI nr
Match: XP_023514499.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 201.4 bits (511), Expect = 7.5e-48
Identity = 103/214 (48.13%), Postives = 131/214 (61.21%), Query Frame = 0


Query: 3   MIMLLLLFNLMLTASYEFEYFQMVQQWAPTFCSGIGVTCKVQPPQNKFTIHGLWPSNFSN 62
           ++ +  L  L L    +F+YFQMVQQW P  CS  GV C   PP   FTIHGLWPSN+SN
Sbjct: 7   IVSIFNLALLTLCVKAQFDYFQMVQQWPPATCS--GVRCYANPPA-MFTIHGLWPSNYSN 66

Query: 63  AKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGNDFDFWRYEWRKHGRCSNPPLNPNQYL 122
             LIC+G       +P     +L  +WP++  GN+  FW++EW  HG CS+PP N  QY 
Sbjct: 67  VPLICTGQPFNPTLVP-----QLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYF 126

Query: 123 HTT--IRMYLNYDLLGMLNAAGLSPS-TTFRQYTYMENVLKNAIGAVPSLRCNVNSQSNE 182
            TT  IR   NYDLL +LN AGL P+ T  RQY  +E  ++ A G  P LRCN N+QS +
Sbjct: 127 QTTLNIRTNHNYDLLAILNTAGLGPTGTNTRQYGAIEGAIQAATGKKPGLRCNKNAQSKK 186

Query: 183 DQLYEIVICFEENSTNLIDCTYAPG-NCPDWFVW 213
            QL+EI++CF++N   LIDCT   G  CP  FVW
Sbjct: 187 KQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVW 212

[Close]

BLAST of CmoCh05G012420 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.6e-27
Identity = 65/215 (30.23%), Postives = 109/215 (50.70%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTA--SYEFEYFQMVQQWAPTFCSGIGVTC--KVQPPQNKFTIHGLW 60
           +++++ LL+F  +  +    +F++F  V QW   +C      C      P   F IHGLW
Sbjct: 7   VNVLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLW 66

Query: 61  PS-NFSNAKLICSGGDLQQNKLPHRLSTKLKKHWPNLKCGND--FDFWRYEWRKHGRCSN 120
           P+ N  +    C             L + LKK+WP L C ++  F+FW +EW KHG CS 
Sbjct: 67  PNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSE 126

Query: 121 PPLNPNQYLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNV 180
             ++ ++Y    +++    +LL +L  +G++P   F     + N +K+ IG  P + CN 
Sbjct: 127 SVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNK 186

Query: 181 NSQSNEDQLYEIVICFEENSTNLIDCTYAP-GNCP 208
           + + N  QL++I IC + + T  I+C   P G+CP
Sbjct: 187 DPERNA-QLHQIYICVDTSGTEFIECPVLPRGSCP 220

[Close]

BLAST of CmoCh05G012420 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 116.7 bits (291), Expect = 2.3e-26
Identity = 67/216 (31.02%), Postives = 106/216 (49.07%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTA---SYEFEYFQMVQQWAPTFCSGIGVTC--KVQPPQNKFTIHGL 60
           + +I LL++   + +A   S +F++F  VQQW  ++C      C      P   F IHGL
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGL 67

Query: 61  WPSNFSNAKL--ICSGGDLQQNKLPHRLSTKLKKHWPNLKC--GNDFDFWRYEWRKHGRC 120
           WP N+ +      C       +     L T +KK WP L C  G+   FW +EW KHG C
Sbjct: 68  WP-NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 127

Query: 121 SNPPLNPNQYLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRC 180
           S   ++ ++Y  T + +    +LLG L  AG++P         + + +K +IG  P + C
Sbjct: 128 SESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVEC 187

Query: 181 NVNSQSNEDQLYEIVICFEENSTNLIDCTYAP-GNC 207
           N +   N  QLY++ +C + + + LI+C   P G C
Sbjct: 188 NRDGSGN-SQLYQVYLCVDRSGSGLIECPVFPHGKC 221

[Close]

BLAST of CmoCh05G012420 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 113.2 bits (282), Expect = 2.5e-25
Identity = 64/214 (29.91%), Postives = 102/214 (47.66%), Query Frame = 0


Query: 1   MSMIMLLLLFNLMLTASY--EFEYFQMVQQWAPTFCSGIGVTCKVQ--PPQNKFTIHGLW 60
           M   + +L    +   S+  +F++F  V QW   +C      C  Q   P   F IHGLW
Sbjct: 1   MKFFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLW 60

Query: 61  PSNFSNA-KLICSGGDLQQNKLPHRLSTKLKKHWPNLKC-GND-FDFWRYEWRKHGRCSN 120
           P+  +      C+      +     L + L++ WP L C  ND   FW +EW KHG C+ 
Sbjct: 61  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 120

Query: 121 PPLNPNQYLHTTIRMYLNYDLLGMLNAAGLSPSTTFRQYTYMENVLKNAIGAVPSLRCNV 180
             L+ + Y    +++    +LL  L  AG+ P   F +   +EN +K  +G  P + CN 
Sbjct: 121 SELDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNK 180

Query: 181 NSQSNEDQLYEIVICFEENSTNLIDCTYAP-GNC 207
           +S S+  QLY+I +C + +++  I+C   P G C
Sbjct: 181 DS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRC 213

The following BLAST results are available for this feature:

BLAST of CmoCh05G012420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	4.5e-27	34.07	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	3.2e-25	31.02	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	3.5e-24	29.91	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P83618	5.6e-22	29.57	Ribonuclease-like storage protein OS=Panax ginseng OX=4054 PE=1 SV=2	[more]
Q40381	1.6e-21	32.71	Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1	[more]

BLAST of CmoCh05G012420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4E7	2.6e-126	100.00	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111450689 PE=3 SV=1	[more]
A0A6J1F837	4.3e-49	48.54	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1	[more]
A0A6J1EXD5	8.1e-48	47.66	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]
A0A6J1IAR7	3.1e-47	47.20	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1	[more]
A0A6J1HV84	4.6e-43	44.55	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111466502 PE=3 SV=1	[more]

BLAST of CmoCh05G012420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022946697.1	5.3e-126	100.00	ribonuclease MC-like [Cucurbita moschata]	[more]
XP_023547237.1	9.9e-125	99.06	ribonuclease MC-like [Cucurbita pepo subsp. pepo]	[more]
XP_038906187.1	1.0e-52	48.60	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022934593.1	8.9e-49	48.54	ribonuclease MC-like [Cucurbita moschata]	[more]
XP_023514499.1	7.5e-48	48.13	ribonuclease MC-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmoCh05G012420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	1.6e-27	30.23	Ribonuclease T2 family protein	[more]
AT2G02990.1	2.3e-26	31.02	ribonuclease 1	[more]
AT1G26820.1	2.5e-25	29.91	ribonuclease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 20..199 e-value: 2.7E-40 score: 138.5
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 5..199
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 13..208 e-value: 1.5E-52 score: 180.6
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 20..211
None	No IPR available	PANTHER	PTHR11240:SF59	RIBONUCLEASE T2 FAMILY PROTEIN	coord: 5..199
None	No IPR available	CDD	cd00374	RNase_T2	coord: 20..208 e-value: 5.68601E-56 score: 174.567
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 100..111
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 50..57

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh05G012420.1	CmoCh05G012420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding