CmoCh05G011210 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G011210
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase At5g03980-like
LocationCmo_Chr05: 8924104 .. 8925226 (+)
RNA-Seq ExpressionCmoCh05G011210
SyntenyCmoCh05G011210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATCACTAATTCTTAACGAATTTTCAGCTTTAGACGCCCGACATCCATTGTTGAATCCTTATCTAAACAAAAATGCTTCCAAGAATCATGGAGTTAACTTCGCAGTGGCTGGTGCCACGGCTTTGCCTTTTGAGCTTCTTTTAACGAAGAATCTCTCTACTTTTTCAACCAATTCTTCTCTCGACCATCAACTTAGTTGGATGTTGTCTCATTTTAGCACAATTTGCCAAAATCAAATAGGTATATTATTTGGACTTTCACCTTCCTTTTTCTCATTACATATGTTTATATTTCTTTACTGTGGTAGAATGTCGCAACACGTTAAGGAATGCTTTGTTCATCGTGGGGGAGATTGGCGGGAATGATTATAATTTTGCGTTGTTAATGGGAAGAACTTTTCAAGAGATAAAAGATATGGTTCCTGATGTTGTTCAAACTATAAAGAGGGTTGTTGAGGTAATTGTGTTTTGATTTTGATTTTTGTTAGTCGTATTATGAATTGATCGTTTTTTTTTTTTTTTTTTGTTGGATGGAGTTCACAGAAAGTGATAAGTCACGGTGCTACTCGAGTTATCATTCCTGGAAACATTCCAATTGGATGTTTTCCTCTCTTTCTCACTATTTTTCAAACCAACGATACAACTGCTTATGATGAATTTCGATGTTTAAAAGATCTAAATGCTTTGGCAATTTATCACAATCATCAAATCAAGCGAGTCATTAGAGAATTGAGAAAGGAGAATCCTCATACTATTATTTTGTACGGTGATTATTATAATGCATTTCGATGGATTCTTCGTCGTGCTTCCAATCTTGGTACGTCAATTCAAAACTTTGAAGTTATTTTGCATTATTCTTAATACACAAAAATTACATATGAGATCTCAATGTTCATATTTGTGAGCAGGATTTGATGAAGATTCTTTGCAAAAATCATGTTGTGGAATTGGAGGCGACTACAACTTTGACCTGAAGAACATATGTGGACCTGAAGTACCTGTTTGTCCAAACCCTAATGACCATATTAGTTGGGATGGAGTCCACTTGACACAAAATGCTTATAAGTTCATGGCACATTGGCTCATCGACGACATTGTCCCAAAGTTGAGATGTATTGTTTAA

mRNA sequence

CGATCACTAATTCTTAACGAATTTTCAGCTTTAGACGCCCGACATCCATTGTTGAATCCTTATCTAAACAAAAATGCTTCCAAGAATCATGGAGTTAACTTCGCAGTGGCTGGTGCCACGGCTTTGCCTTTTGAGCTTCTTTTAACGAAGAATCTCTCTACTTTTTCAACCAATTCTTCTCTCGACCATCAACTTAGTTGGATGTTGTCTCATTTTAGCACAATTTGCCAAAATCAAATAGAATGTCGCAACACGTTAAGGAATGCTTTGTTCATCGTGGGGGAGATTGGCGGGAATGATTATAATTTTGCGTTGTTAATGGGAAGAACTTTTCAAGAGATAAAAGATATGGTTCCTGATGTTGTTCAAACTATAAAGAGGGTTGTTGAGAAAGTGATAAGTCACGGTGCTACTCGAGTTATCATTCCTGGAAACATTCCAATTGGATGTTTTCCTCTCTTTCTCACTATTTTTCAAACCAACGATACAACTGCTTATGATGAATTTCGATGTTTAAAAGATCTAAATGCTTTGGCAATTTATCACAATCATCAAATCAAGCGAGTCATTAGAGAATTGAGAAAGGAGAATCCTCATACTATTATTTTGTACGGTGATTATTATAATGCATTTCGATGGATTCTTCGTCGTGCTTCCAATCTTGGATTTGATGAAGATTCTTTGCAAAAATCATGTTGTGGAATTGGAGGCGACTACAACTTTGACCTGAAGAACATATGTGGACCTGAAGTACCTGTTTGTCCAAACCCTAATGACCATATTAGTTGGGATGGAGTCCACTTGACACAAAATGCTTATAAGTTCATGGCACATTGGCTCATCGACGACATTGTCCCAAAGTTGAGATGTATTGTTTAA

Coding sequence (CDS)

CGATCACTAATTCTTAACGAATTTTCAGCTTTAGACGCCCGACATCCATTGTTGAATCCTTATCTAAACAAAAATGCTTCCAAGAATCATGGAGTTAACTTCGCAGTGGCTGGTGCCACGGCTTTGCCTTTTGAGCTTCTTTTAACGAAGAATCTCTCTACTTTTTCAACCAATTCTTCTCTCGACCATCAACTTAGTTGGATGTTGTCTCATTTTAGCACAATTTGCCAAAATCAAATAGAATGTCGCAACACGTTAAGGAATGCTTTGTTCATCGTGGGGGAGATTGGCGGGAATGATTATAATTTTGCGTTGTTAATGGGAAGAACTTTTCAAGAGATAAAAGATATGGTTCCTGATGTTGTTCAAACTATAAAGAGGGTTGTTGAGAAAGTGATAAGTCACGGTGCTACTCGAGTTATCATTCCTGGAAACATTCCAATTGGATGTTTTCCTCTCTTTCTCACTATTTTTCAAACCAACGATACAACTGCTTATGATGAATTTCGATGTTTAAAAGATCTAAATGCTTTGGCAATTTATCACAATCATCAAATCAAGCGAGTCATTAGAGAATTGAGAAAGGAGAATCCTCATACTATTATTTTGTACGGTGATTATTATAATGCATTTCGATGGATTCTTCGTCGTGCTTCCAATCTTGGATTTGATGAAGATTCTTTGCAAAAATCATGTTGTGGAATTGGAGGCGACTACAACTTTGACCTGAAGAACATATGTGGACCTGAAGTACCTGTTTGTCCAAACCCTAATGACCATATTAGTTGGGATGGAGTCCACTTGACACAAAATGCTTATAAGTTCATGGCACATTGGCTCATCGACGACATTGTCCCAAAGTTGAGATGTATTGTTTAA

Protein sequence

RSLILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLDHQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVVQTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYHNHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFDLKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV
Homology
BLAST of CmoCh05G011210 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.3e-66
Identity = 128/291 (43.99%), Postives = 169/291 (58.08%), Query Frame = 0

Query: 4   ILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLDH 63
           ++ +F A     PLLNPYL +N S  HGVNFAVAGATAL    L  + +     +S L  
Sbjct: 77  LIIDFIATALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHSHLSA 136

Query: 64  QLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVVQ 123
           QL+W  ++  +IC    EC N L+NALFI+G IG ND N+A    RT +EI+  VP + +
Sbjct: 137 QLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVPFITE 196

Query: 124 TIKRVVEKVISHGATRVIIPGNIPIGCFPL---FLTIFQTNDTTAYDEFRCLKDLNALAI 183
            +     ++I  G +RVI+PG  PIGC      FL  F   D    D+  CL  LN L+I
Sbjct: 197 AVANATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDK---DDLGCLSSLNNLSI 256

Query: 184 YHNHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYN 243
           Y N   +R +  L  E P  +I+Y DYYNA+R++ R    LG +  SL K CCGIGG YN
Sbjct: 257 YFNSLFQRALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYN 316

Query: 244 FDLKNICGPE-VPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRC 291
           +D    CG   VPVCPNP  +I WDG H TQ AY+ +A ++I  I+  L+C
Sbjct: 317 YDPDRECGSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKC 363

BLAST of CmoCh05G011210 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 5.7e-65
Identity = 120/260 (46.15%), Postives = 163/260 (62.69%), Query Frame = 0

Query: 32  VNFAVAGATALPFELLLTKNLSTFSTNSSLDHQLSWMLSHFSTICQNQIECRNTLRNALF 91
           VNF V+G+TAL       +NL   +TN+ L  QL+W   H  + C       + L+++LF
Sbjct: 66  VNFGVSGSTALNSSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCHG--SSSDCLKHSLF 125

Query: 92  IVGEIGGNDYNFALLMGRTFQEIKDMVPDVVQTIKRVVEKVISHGATRVIIPGNIPIGCF 151
           +VGEIGGNDYN+    G+  +EI+  +P VV  I     +VI  GA  V++PGN P+GCF
Sbjct: 126 MVGEIGGNDYNYGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCF 185

Query: 152 PLFLTIFQTNDTTAYDEFRCLKDLNALAIYHNHQIKRVIRELRKENPHTIILYGDYYNAF 211
           P++LT F   DT  YD+  CL  LN  A+ HN+Q++  I  LRKE P   I+YGDYYNAF
Sbjct: 186 PIYLTSFPVKDTKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAF 245

Query: 212 RWILRRASNLGFDEDSLQKSCCGIGGDYNFDLKNICGP-EVPVCPNPNDHISWDGVHLTQ 271
           +++LR      FD+    KSCCG GG YN+D K   G   VPVC NP+  ISWDGVHLTQ
Sbjct: 246 QYVLRSER---FDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQ 305

Query: 272 NAYKFMAHWLIDDIVPKLRC 291
            AY+FM+ +L + I+ +++C
Sbjct: 306 KAYRFMSKFLNNQILSQIKC 320

BLAST of CmoCh05G011210 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.5e-54
Identity = 107/281 (38.08%), Postives = 167/281 (59.43%), Query Frame = 0

Query: 4   ILNEFSALDARHPLLNP-YLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 63
           ++ +F A     PL+ P Y ++NA+   GVNFAV GATAL    L  + +    TN SL 
Sbjct: 82  LIIDFIAEFLGFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLA 141

Query: 64  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 123
            QLS        +C +  +CR+ + N+L ++GEIGGNDYN+A  +G+  +EIK++VP V+
Sbjct: 142 VQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVI 201

Query: 124 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFR-CLKDLNALAIY 183
           +TI   + ++I  G    ++PG  P+GC   +L+++QT++   YD    CLK LN  + Y
Sbjct: 202 ETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEY 261

Query: 184 HNHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNF 243
           H+ Q++  +  L+K  PH  I+Y DYYN    + +  +  GF    L  +CC +GG +NF
Sbjct: 262 HDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPL-PACCALGGPFNF 321

Query: 244 DLKNICGPEVP-VCPNPNDHISWDGVHLTQNAYKFMAHWLI 282
            L    G +VP  C +P+ ++SWDGVH+T+ AY+ MA  ++
Sbjct: 322 TLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMAEGIL 361

BLAST of CmoCh05G011210 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 209.1 bits (531), Expect = 6.5e-53
Identity = 105/281 (37.37%), Postives = 166/281 (59.07%), Query Frame = 0

Query: 4   ILNEFSALDARHPLLNPYLN-KNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 63
           ++ +F A     PL+ P+   +NA+   GVNFAVAGATAL    L  + + +  TN SL 
Sbjct: 84  LIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLS 143

Query: 64  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 123
            QL         +C +  +CR+ + NAL ++GEIGGNDYNFAL   +  +E++++VP V+
Sbjct: 144 VQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVI 203

Query: 124 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFR-CLKDLNALAIY 183
            TI   + +++  G    ++PGN PIG    +LT+++T++   YD    CLK LN  + Y
Sbjct: 204 ATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEY 263

Query: 184 HNHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNF 243
           +N Q++  +  LRK  PH  I+Y DYYNA   + +  +  GF    L  +CCG+GG YNF
Sbjct: 264 YNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNF 323

Query: 244 DLKNICGP-EVPVCPNPNDHISWDGVHLTQNAYKFMAHWLI 282
           +    CG   V  C +P+ ++++DG+H+T+ AY+ ++  L+
Sbjct: 324 NFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of CmoCh05G011210 vs. ExPASy Swiss-Prot
Match: Q3E7I6 (GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-52
Identity = 105/271 (38.75%), Postives = 160/271 (59.04%), Query Frame = 0

Query: 16  PLLNPYL-NKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLDHQLSWMLSHFST 75
           P + PY  ++N S N G+NFAV GATAL    L+ + + +  TN SL  QL+        
Sbjct: 97  PYVPPYFGSQNVSFNQGINFAVYGATALDRAFLVKQGIKSDFTNISLSVQLNTFKQILPN 156

Query: 76  ICQNQI-ECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVVQTIKRVVEKVI 135
           +C +   +CR  L ++L ++GEIGGNDYN+    G++  EIK++VP +++ I   +  +I
Sbjct: 157 LCASSTRDCREMLGDSLILMGEIGGNDYNYPFFEGKSINEIKELVPLIIKAISSAIVDLI 216

Query: 136 SHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFR-CLKDLNALAIYHNHQIKRVIRE 195
             G    ++PGN PIGC   +LT+FQT  T  +D F  C+  LN    +HN Q+K  +++
Sbjct: 217 DLGGKTFLVPGNFPIGCSTAYLTLFQT-ATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQ 276

Query: 196 LRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFDLKNICGPE-V 255
           L+K  PH  I+Y DYYN+   + +  +  GF    L  +CCG+GG YNF +   CG   V
Sbjct: 277 LQKLYPHVNIIYADYYNSLYGLFQEPAKYGFKNRPL-AACCGVGGQYNFTIGKECGENGV 336

Query: 256 PVCPNPNDHISWDGVHLTQNAYKFMAHWLID 283
             C NP+++++WDG HLT+  Y+ MA  L++
Sbjct: 337 SYCQNPSEYVNWDGYHLTEATYQKMAQGLLN 365

BLAST of CmoCh05G011210 vs. ExPASy TrEMBL
Match: A0A6J1G566 (acetylajmalan esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111450819 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.1e-167
Identity = 284/290 (97.93%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L++ ++ ALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD
Sbjct: 80  LLMIDYFALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 139

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV
Sbjct: 140 HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 199

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH
Sbjct: 200 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 259

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD
Sbjct: 260 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 319

Query: 243 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 293
           LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV
Sbjct: 320 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 369

BLAST of CmoCh05G011210 vs. ExPASy TrEMBL
Match: A0A6J1KIA1 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493496 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 4.4e-161
Identity = 274/290 (94.48%), Postives = 282/290 (97.24%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L++ ++ ALDAR PLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLS+FSTNSSLD
Sbjct: 80  LLMIDYFALDARLPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSSFSTNSSLD 139

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQLSWMLSHFSTIC+NQIECRN LRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV
Sbjct: 140 HQLSWMLSHFSTICRNQIECRNKLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 199

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIKRVVEKVISHGATRVI+PGNIPIGCFPLFLTIFQTNDTTAYDE RCLKDLNALAIYH
Sbjct: 200 QTIKRVVEKVISHGATRVIVPGNIPIGCFPLFLTIFQTNDTTAYDEHRCLKDLNALAIYH 259

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           NHQIKRVI+ELR ENP TIILYGDYYNAFRWIL RASNLGFDEDSLQKSCCGIGGDYNFD
Sbjct: 260 NHQIKRVIKELRNENPRTIILYGDYYNAFRWILCRASNLGFDEDSLQKSCCGIGGDYNFD 319

Query: 243 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 293
           LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV
Sbjct: 320 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 369

BLAST of CmoCh05G011210 vs. ExPASy TrEMBL
Match: A0A6J1DCG0 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111018810 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 4.2e-119
Identity = 204/290 (70.34%), Postives = 239/290 (82.41%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L+  ++ ALDA  PL+NPYLN++A  NHGVNFAVAG+TAL FE LL   + +  TNSSLD
Sbjct: 48  LLXLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKIFSPVTNSSLD 107

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQL WM SHF++IC N+ +C   LR+ALF+VGEIGGNDYN+AL  G+T QE+KDMVPD+V
Sbjct: 108 HQLGWMHSHFNSICLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTMQEVKDMVPDIV 167

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIK  VEKVIS+GATRVIIPGN PIGC P++LT FQTNDTTAYDE  CLK LN+LA YH
Sbjct: 168 QTIKNAVEKVISYGATRVIIPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLASYH 227

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           N +IK+VI  LR ENPHT+I+YGDYYNA  WILR A  LGFDE SLQKSCCGIGG+YNFD
Sbjct: 228 NDRIKQVIEVLRMENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFD 287

Query: 243 LKNICG-PEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCI 292
           L  ICG P VPVCPNP++HISWDG+HLT+ AYKFMA+W+I+DI PKL CI
Sbjct: 288 LMKICGLPGVPVCPNPHEHISWDGIHLTEKAYKFMAYWIINDIFPKLHCI 337

BLAST of CmoCh05G011210 vs. ExPASy TrEMBL
Match: A0A6J1DQL3 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111023320 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 4.2e-119
Identity = 204/290 (70.34%), Postives = 238/290 (82.07%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L++ ++ ALDA  PL+NPYLN++A  NHGVNFAVAG+TAL FE LL   + +  TNSSLD
Sbjct: 81  LLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKILSPVTNSSLD 140

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQL WM SHF +IC N+ +C   LR+ALF+VGEIGGNDYN+AL  G+T QE+KDMVPDVV
Sbjct: 141 HQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVV 200

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIK  VEKVIS+GATRVI+PGN PIGC P++LT FQTNDTTAYDE  CLK LN+LA YH
Sbjct: 201 QTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLASYH 260

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           N QIK+ I  L++ENPHT+I+YGDYYNA  WILR A  LGFDE SLQKSCCGIGG+YNF+
Sbjct: 261 NDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFN 320

Query: 243 LKNICG-PEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCI 292
           L  ICG P VPVCPNP++HISWDG+HLTQ AYKFMA+WLI DI PKL CI
Sbjct: 321 LMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLIHDIFPKLHCI 370

BLAST of CmoCh05G011210 vs. ExPASy TrEMBL
Match: A0A5A7VJA5 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00480 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.3e-117
Identity = 201/292 (68.84%), Postives = 238/292 (81.51%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTK-NLSTFSTNSSL 62
           L++ +F ALDA   L++PYLNK+ S +HGVNFAVAG+TALP + L T   + +  TNSSL
Sbjct: 48  LLMLDFFALDAGLSLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSSL 107

Query: 63  DHQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDV 122
           DHQL WM SHF++IC NQ +C   LR+ALF+VGEIGGNDYN+AL  G+T +E KDMVPDV
Sbjct: 108 DHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPDV 167

Query: 123 VQTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIY 182
           VQTIK  VEKVIS+GATRV++PGN PIGCFP++LT F TNDT+AYDE  CLKDLN LA Y
Sbjct: 168 VQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLATY 227

Query: 183 HNHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNF 242
           HN+QIK+ I  L+KENPHT+I+YGDYYNAF WI+R A  LGFDE+SLQKSCCGIGG+Y F
Sbjct: 228 HNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGEYRF 287

Query: 243 DLKNICGPE-VPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 293
           +L  +CG E V VCPNPN+HISWDGVHLTQ+ YKFM HWLI DI PKL CIV
Sbjct: 288 NLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

BLAST of CmoCh05G011210 vs. NCBI nr
Match: XP_022946879.1 (acetylajmalan esterase-like [Cucurbita moschata])

HSP 1 Score: 599.0 bits (1543), Expect = 2.2e-167
Identity = 284/290 (97.93%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L++ ++ ALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD
Sbjct: 80  LLMIDYFALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 139

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV
Sbjct: 140 HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 199

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH
Sbjct: 200 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 259

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD
Sbjct: 260 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 319

Query: 243 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 293
           LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV
Sbjct: 320 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 369

BLAST of CmoCh05G011210 vs. NCBI nr
Match: XP_023546451.1 (acetylajmalan esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.6 bits (1503), Expect = 9.7e-163
Identity = 278/290 (95.86%), Postives = 284/290 (97.93%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L++ ++ ALDAR PLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLST STNSSLD
Sbjct: 80  LLMIDYFALDARLPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTSSTNSSLD 139

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQLSWMLSHFSTI QNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQE+KDMVPDVV
Sbjct: 140 HQLSWMLSHFSTIYQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEVKDMVPDVV 199

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIKRVVE+VISHGATRVIIPGNIPIGCFPLFLTIFQTND TAYDEFRCLKDLNALAIYH
Sbjct: 200 QTIKRVVERVISHGATRVIIPGNIPIGCFPLFLTIFQTNDITAYDEFRCLKDLNALAIYH 259

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD
Sbjct: 260 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 319

Query: 243 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 293
           LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV
Sbjct: 320 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 369

BLAST of CmoCh05G011210 vs. NCBI nr
Match: XP_022998968.1 (acetylajmalan esterase-like [Cucurbita maxima])

HSP 1 Score: 577.0 bits (1486), Expect = 9.1e-161
Identity = 274/290 (94.48%), Postives = 282/290 (97.24%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L++ ++ ALDAR PLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLS+FSTNSSLD
Sbjct: 80  LLMIDYFALDARLPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSSFSTNSSLD 139

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQLSWMLSHFSTIC+NQIECRN LRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV
Sbjct: 140 HQLSWMLSHFSTICRNQIECRNKLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 199

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIKRVVEKVISHGATRVI+PGNIPIGCFPLFLTIFQTNDTTAYDE RCLKDLNALAIYH
Sbjct: 200 QTIKRVVEKVISHGATRVIVPGNIPIGCFPLFLTIFQTNDTTAYDEHRCLKDLNALAIYH 259

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           NHQIKRVI+ELR ENP TIILYGDYYNAFRWIL RASNLGFDEDSLQKSCCGIGGDYNFD
Sbjct: 260 NHQIKRVIKELRNENPRTIILYGDYYNAFRWILCRASNLGFDEDSLQKSCCGIGGDYNFD 319

Query: 243 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 293
           LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV
Sbjct: 320 LKNICGPEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCIV 369

BLAST of CmoCh05G011210 vs. NCBI nr
Match: XP_022156428.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 437.6 bits (1124), Expect = 8.6e-119
Identity = 204/290 (70.34%), Postives = 238/290 (82.07%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L++ ++ ALDA  PL+NPYLN++A  NHGVNFAVAG+TAL FE LL   + +  TNSSLD
Sbjct: 81  LLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKILSPVTNSSLD 140

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQL WM SHF +IC N+ +C   LR+ALF+VGEIGGNDYN+AL  G+T QE+KDMVPDVV
Sbjct: 141 HQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVV 200

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIK  VEKVIS+GATRVI+PGN PIGC P++LT FQTNDTTAYDE  CLK LN+LA YH
Sbjct: 201 QTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLASYH 260

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           N QIK+ I  L++ENPHT+I+YGDYYNA  WILR A  LGFDE SLQKSCCGIGG+YNF+
Sbjct: 261 NDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFN 320

Query: 243 LKNICG-PEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCI 292
           L  ICG P VPVCPNP++HISWDG+HLTQ AYKFMA+WLI DI PKL CI
Sbjct: 321 LMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLIHDIFPKLHCI 370

BLAST of CmoCh05G011210 vs. NCBI nr
Match: XP_022150751.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 437.6 bits (1124), Expect = 8.6e-119
Identity = 204/290 (70.34%), Postives = 239/290 (82.41%), Query Frame = 0

Query: 3   LILNEFSALDARHPLLNPYLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 62
           L+  ++ ALDA  PL+NPYLN++A  NHGVNFAVAG+TAL FE LL   + +  TNSSLD
Sbjct: 48  LLXLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKIFSPVTNSSLD 107

Query: 63  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 122
           HQL WM SHF++IC N+ +C   LR+ALF+VGEIGGNDYN+AL  G+T QE+KDMVPD+V
Sbjct: 108 HQLGWMHSHFNSICLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTMQEVKDMVPDIV 167

Query: 123 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFRCLKDLNALAIYH 182
           QTIK  VEKVIS+GATRVIIPGN PIGC P++LT FQTNDTTAYDE  CLK LN+LA YH
Sbjct: 168 QTIKNAVEKVISYGATRVIIPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLASYH 227

Query: 183 NHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFD 242
           N +IK+VI  LR ENPHT+I+YGDYYNA  WILR A  LGFDE SLQKSCCGIGG+YNFD
Sbjct: 228 NDRIKQVIEVLRMENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFD 287

Query: 243 LKNICG-PEVPVCPNPNDHISWDGVHLTQNAYKFMAHWLIDDIVPKLRCI 292
           L  ICG P VPVCPNP++HISWDG+HLT+ AYKFMA+W+I+DI PKL CI
Sbjct: 288 LMKICGLPGVPVCPNPHEHISWDGIHLTEKAYKFMAYWIINDIFPKLHCI 337

BLAST of CmoCh05G011210 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.0e-66
Identity = 120/260 (46.15%), Postives = 163/260 (62.69%), Query Frame = 0

Query: 32  VNFAVAGATALPFELLLTKNLSTFSTNSSLDHQLSWMLSHFSTICQNQIECRNTLRNALF 91
           VNF V+G+TAL       +NL   +TN+ L  QL+W   H  + C       + L+++LF
Sbjct: 66  VNFGVSGSTALNSSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCHG--SSSDCLKHSLF 125

Query: 92  IVGEIGGNDYNFALLMGRTFQEIKDMVPDVVQTIKRVVEKVISHGATRVIIPGNIPIGCF 151
           +VGEIGGNDYN+    G+  +EI+  +P VV  I     +VI  GA  V++PGN P+GCF
Sbjct: 126 MVGEIGGNDYNYGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCF 185

Query: 152 PLFLTIFQTNDTTAYDEFRCLKDLNALAIYHNHQIKRVIRELRKENPHTIILYGDYYNAF 211
           P++LT F   DT  YD+  CL  LN  A+ HN+Q++  I  LRKE P   I+YGDYYNAF
Sbjct: 186 PIYLTSFPVKDTKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAF 245

Query: 212 RWILRRASNLGFDEDSLQKSCCGIGGDYNFDLKNICGP-EVPVCPNPNDHISWDGVHLTQ 271
           +++LR      FD+    KSCCG GG YN+D K   G   VPVC NP+  ISWDGVHLTQ
Sbjct: 246 QYVLRSER---FDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQ 305

Query: 272 NAYKFMAHWLIDDIVPKLRC 291
            AY+FM+ +L + I+ +++C
Sbjct: 306 KAYRFMSKFLNNQILSQIKC 320

BLAST of CmoCh05G011210 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.2e-55
Identity = 107/281 (38.08%), Postives = 167/281 (59.43%), Query Frame = 0

Query: 4   ILNEFSALDARHPLLNP-YLNKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 63
           ++ +F A     PL+ P Y ++NA+   GVNFAV GATAL    L  + +    TN SL 
Sbjct: 82  LIIDFIAEFLGFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLA 141

Query: 64  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 123
            QLS        +C +  +CR+ + N+L ++GEIGGNDYN+A  +G+  +EIK++VP V+
Sbjct: 142 VQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVI 201

Query: 124 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFR-CLKDLNALAIY 183
           +TI   + ++I  G    ++PG  P+GC   +L+++QT++   YD    CLK LN  + Y
Sbjct: 202 ETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEY 261

Query: 184 HNHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNF 243
           H+ Q++  +  L+K  PH  I+Y DYYN    + +  +  GF    L  +CC +GG +NF
Sbjct: 262 HDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPL-PACCALGGPFNF 321

Query: 244 DLKNICGPEVP-VCPNPNDHISWDGVHLTQNAYKFMAHWLI 282
            L    G +VP  C +P+ ++SWDGVH+T+ AY+ MA  ++
Sbjct: 322 TLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMAEGIL 361

BLAST of CmoCh05G011210 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 209.1 bits (531), Expect = 4.6e-54
Identity = 105/281 (37.37%), Postives = 166/281 (59.07%), Query Frame = 0

Query: 4   ILNEFSALDARHPLLNPYLN-KNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 63
           ++ +F A     PL+ P+   +NA+   GVNFAVAGATAL    L  + + +  TN SL 
Sbjct: 84  LIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLS 143

Query: 64  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 123
            QL         +C +  +CR+ + NAL ++GEIGGNDYNFAL   +  +E++++VP V+
Sbjct: 144 VQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVI 203

Query: 124 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFR-CLKDLNALAIY 183
            TI   + +++  G    ++PGN PIG    +LT+++T++   YD    CLK LN  + Y
Sbjct: 204 ATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEY 263

Query: 184 HNHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNF 243
           +N Q++  +  LRK  PH  I+Y DYYNA   + +  +  GF    L  +CCG+GG YNF
Sbjct: 264 YNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNF 323

Query: 244 DLKNICGP-EVPVCPNPNDHISWDGVHLTQNAYKFMAHWLI 282
           +    CG   V  C +P+ ++++DG+H+T+ AY+ ++  L+
Sbjct: 324 NFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of CmoCh05G011210 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 208.4 bits (529), Expect = 7.9e-54
Identity = 105/271 (38.75%), Postives = 160/271 (59.04%), Query Frame = 0

Query: 16  PLLNPYL-NKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLDHQLSWMLSHFST 75
           P + PY  ++N S N G+NFAV GATAL    L+ + + +  TN SL  QL+        
Sbjct: 97  PYVPPYFGSQNVSFNQGINFAVYGATALDRAFLVKQGIKSDFTNISLSVQLNTFKQILPN 156

Query: 76  ICQNQI-ECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVVQTIKRVVEKVI 135
           +C +   +CR  L ++L ++GEIGGNDYN+    G++  EIK++VP +++ I   +  +I
Sbjct: 157 LCASSTRDCREMLGDSLILMGEIGGNDYNYPFFEGKSINEIKELVPLIIKAISSAIVDLI 216

Query: 136 SHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYDEFR-CLKDLNALAIYHNHQIKRVIRE 195
             G    ++PGN PIGC   +LT+FQT  T  +D F  C+  LN    +HN Q+K  +++
Sbjct: 217 DLGGKTFLVPGNFPIGCSTAYLTLFQT-ATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQ 276

Query: 196 LRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNFDLKNICGPE-V 255
           L+K  PH  I+Y DYYN+   + +  +  GF    L  +CCG+GG YNF +   CG   V
Sbjct: 277 LQKLYPHVNIIYADYYNSLYGLFQEPAKYGFKNRPL-AACCGVGGQYNFTIGKECGENGV 336

Query: 256 PVCPNPNDHISWDGVHLTQNAYKFMAHWLID 283
             C NP+++++WDG HLT+  Y+ MA  L++
Sbjct: 337 SYCQNPSEYVNWDGYHLTEATYQKMAQGLLN 365

BLAST of CmoCh05G011210 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 208.4 bits (529), Expect = 7.9e-54
Identity = 108/282 (38.30%), Postives = 164/282 (58.16%), Query Frame = 0

Query: 4   ILNEFSALDARHPLLNPYL-NKNASKNHGVNFAVAGATALPFELLLTKNLSTFSTNSSLD 63
           I+ +F A     P + PY  +KN + + GVNFAVAGATAL    L  + +    TN SL 
Sbjct: 79  IIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQP-HTNVSLG 138

Query: 64  HQLSWMLSHFSTICQNQIECRNTLRNALFIVGEIGGNDYNFALLMGRTFQEIKDMVPDVV 123
            QL         +C +  +CR+ + NAL ++GEIGGNDYNF     +  +E++++VP V+
Sbjct: 139 VQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVI 198

Query: 124 QTIKRVVEKVISHGATRVIIPGNIPIGCFPLFLTIFQTNDTTAYD-EFRCLKDLNALAIY 183
            +I   + ++I  G    ++PG  PIGC  ++LT+++T++   YD    CLK LN    Y
Sbjct: 199 ASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEY 258

Query: 184 HNHQIKRVIRELRKENPHTIILYGDYYNAFRWILRRASNLGFDEDSLQKSCCGIGGDYNF 243
           H+ ++K  +  LRK  PH  I+Y DYYN+   I +  +  GF E     +CCGIGG YNF
Sbjct: 259 HSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIGGPYNF 318

Query: 244 DLKNICGP-EVPVCPNPNDHISWDGVHLTQNAYKFMAHWLID 283
           +    CG   V  C +P+ ++ WDGVH+T+ AYK++A  +++
Sbjct: 319 NFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIADGILN 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3MKY22.3e-6643.99Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1[more]
Q9LZB25.7e-6546.15GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2... [more]
Q9FXJ14.5e-5438.08GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3... [more]
Q8RXT96.5e-5337.37GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2... [more]
Q3E7I61.1e-5238.75GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1G5661.1e-16797.93acetylajmalan esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111450819 PE=3 S... [more]
A0A6J1KIA14.4e-16194.48acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493496 PE=3 SV=... [more]
A0A6J1DCG04.2e-11970.34acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111018810 PE=3 ... [more]
A0A6J1DQL34.2e-11970.34acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111023320 PE=3 ... [more]
A0A5A7VJA51.3e-11768.84Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
XP_022946879.12.2e-16797.93acetylajmalan esterase-like [Cucurbita moschata][more]
XP_023546451.19.7e-16395.86acetylajmalan esterase-like [Cucurbita pepo subsp. pepo][more]
XP_022998968.19.1e-16194.48acetylajmalan esterase-like [Cucurbita maxima][more]
XP_022156428.18.6e-11970.34acetylajmalan esterase-like [Momordica charantia][more]
XP_022150751.18.6e-11970.34acetylajmalan esterase-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G03980.14.0e-6646.15SGNH hydrolase-type esterase superfamily protein [more]
AT1G28570.13.2e-5538.08SGNH hydrolase-type esterase superfamily protein [more]
AT1G28590.14.6e-5437.37GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G28650.17.9e-5438.75GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G28600.17.9e-5438.30GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 20..280
e-value: 9.3E-22
score: 78.6
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 3..286
e-value: 3.1E-53
score: 183.3
NoneNo IPR availablePANTHERPTHR22835:SF581ACETYLAJMALAN ESTERASE-LIKEcoord: 3..290
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 3..290
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 32..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G011210.1CmoCh05G011210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds