CmoCh05G010850 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G010850
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionnicotianamine synthase-like
LocationCmo_Chr05: 8710688 .. 8712005 (-)
RNA-Seq ExpressionCmoCh05G010850
SyntenyCmoCh05G010850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGCCCTCTGTTGCGAGAAGTGTGCGCCCTGTACGACCAAATCTCGAGCCTTGCTAGCCTCAAGCCTTCCAAAAATGTCGACACTCTCTTCACCCAACTAGTCCTCACGTGCTCCCCGCCCGCCCCTGGCCTTGATATCGCCTCTTTGCCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATCCGTCTATGCGCCACCGCTGAGGCGCTTCTTGAAGTTCATTTCTCTTCCATTTTGGCTTCCTTTGAGAATCCCATTCATAACTTGTCTATCTTTCCTTATTACTCTAATTACTTGAAGCTCTCTCTTCTCGAGTTCGATATTCTTACCTCTCATTGTCGTCGGGTGCCCTCTAGCGTTGCGTTCGTTGGCTCTGGCCCCCTCCCGCTTTCCTCCATTGTCATGGCGTCCACTCATCTTAAGGGTAACTCAAACGCTAGCTAGCTGGCTAACTAGTATTACATAACAATATGAGATTATGTCACAAGACGTGAGATTCTCAAACGCCTCCCTCGAGATAATCCCAGTTTATTTTTTACCGAATACCGGATTCAGGTTCATCGGCTTAGATACCCTATTAGATTAATATCCTGTTCATCTTATTAAGATCGTGAGAGATTTTCAATTTTTATTTTTAATAAGATCTTCGAGAGCTTGCTGGGTTGAGGTGTGATGTGATAGATTATGGTAGATTTGTTATCTAGCTCTAATACCATGTTAAGAATAATTCATGAACTTTTTCGTCTTATATTTCTCAATAGGTACTATTTTCCACAACTTTGACATCGACCCAGTTGCAAACTCCATGGCGGGGCAGTTAGTTTGCTCGGACTCAGATTTATCAAAACGGATGGTGTTTCACACGACAGACGTAATGGAGGTGACGGAGGCGTTTGGAGAGTACGAGGTGGTGTTTTTAGCAGCATTGGTAGGGTTGGAGGAGGAAGAGAAAGGGAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGAGCTTATTTGATGCTAAGGAGTGCACACGGAGCTAGAGCATTTCTCTACCCTGTTGTTGACGTACGTGACGTCGAAGCATCTGGGTTCGAGATTTTGTCCGTGTTTCACCCTACTGATGAAGTTATAAACTCCGTTGTTATTGCAAGAAAGGTGTCTAACCTAGTTCCTGACAACGATAATTATAACGACAACGACACAGATGATGAAAAACGAGCTGTTTTAGTTAACACCCCAATAATATTGGCCGAGAAGTGCTCGGGGTTTAAAGACTTCGGTCCCATGATCGAGGAGCTTGCCATAGAAGAACAACTTTCTTGA

mRNA sequence

ATGGAAGGCCCTCTGTTGCGAGAAGTGTGCGCCCTGTACGACCAAATCTCGAGCCTTGCTAGCCTCAAGCCTTCCAAAAATGTCGACACTCTCTTCACCCAACTAGTCCTCACGTGCTCCCCGCCCGCCCCTGGCCTTGATATCGCCTCTTTGCCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATCCGTCTATGCGCCACCGCTGAGGCGCTTCTTGAAGTTCATTTCTCTTCCATTTTGGCTTCCTTTGAGAATCCCATTCATAACTTGTCTATCTTTCCTTATTACTCTAATTACTTGAAGCTCTCTCTTCTCGAGTTCGATATTCTTACCTCTCATTGTCGTCGGGTGCCCTCTAGCGTTGCGTTCGTTGGCTCTGGCCCCCTCCCGCTTTCCTCCATTGTCATGGCGTCCACTCATCTTAAGGGTACTATTTTCCACAACTTTGACATCGACCCAGTTGCAAACTCCATGGCGGGGCAGTTAGTTTGCTCGGACTCAGATTTATCAAAACGGATGGTGTTTCACACGACAGACGTAATGGAGGTGACGGAGGCGTTTGGAGAGTACGAGGTGGTGTTTTTAGCAGCATTGGTAGGGTTGGAGGAGGAAGAGAAAGGGAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGAGCTTATTTGATGCTAAGGAGTGCACACGGAGCTAGAGCATTTCTCTACCCTGTTGTTGACGTACGTGACGTCGAAGCATCTGGGTTCGAGATTTTGTCCGTGTTTCACCCTACTGATGAAGTTATAAACTCCGTTGTTATTGCAAGAAAGGTGTCTAACCTAGTTCCTGACAACGATAATTATAACGACAACGACACAGATGATGAAAAACGAGCTGTTTTAGTTAACACCCCAATAATATTGGCCGAGAAGTGCTCGGGGTTTAAAGACTTCGGTCCCATGATCGAGGAGCTTGCCATAGAAGAACAACTTTCTTGA

Coding sequence (CDS)

ATGGAAGGCCCTCTGTTGCGAGAAGTGTGCGCCCTGTACGACCAAATCTCGAGCCTTGCTAGCCTCAAGCCTTCCAAAAATGTCGACACTCTCTTCACCCAACTAGTCCTCACGTGCTCCCCGCCCGCCCCTGGCCTTGATATCGCCTCTTTGCCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATCCGTCTATGCGCCACCGCTGAGGCGCTTCTTGAAGTTCATTTCTCTTCCATTTTGGCTTCCTTTGAGAATCCCATTCATAACTTGTCTATCTTTCCTTATTACTCTAATTACTTGAAGCTCTCTCTTCTCGAGTTCGATATTCTTACCTCTCATTGTCGTCGGGTGCCCTCTAGCGTTGCGTTCGTTGGCTCTGGCCCCCTCCCGCTTTCCTCCATTGTCATGGCGTCCACTCATCTTAAGGGTACTATTTTCCACAACTTTGACATCGACCCAGTTGCAAACTCCATGGCGGGGCAGTTAGTTTGCTCGGACTCAGATTTATCAAAACGGATGGTGTTTCACACGACAGACGTAATGGAGGTGACGGAGGCGTTTGGAGAGTACGAGGTGGTGTTTTTAGCAGCATTGGTAGGGTTGGAGGAGGAAGAGAAAGGGAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGAGCTTATTTGATGCTAAGGAGTGCACACGGAGCTAGAGCATTTCTCTACCCTGTTGTTGACGTACGTGACGTCGAAGCATCTGGGTTCGAGATTTTGTCCGTGTTTCACCCTACTGATGAAGTTATAAACTCCGTTGTTATTGCAAGAAAGGTGTCTAACCTAGTTCCTGACAACGATAATTATAACGACAACGACACAGATGATGAAAAACGAGCTGTTTTAGTTAACACCCCAATAATATTGGCCGAGAAGTGCTCGGGGTTTAAAGACTTCGGTCCCATGATCGAGGAGCTTGCCATAGAAGAACAACTTTCTTGA

Protein sequence

MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPIILAEKCSGFKDFGPMIEELAIEEQLS
Homology
[Close]
BLAST of CmoCh05G010850 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.2e-91
Identity = 166/269 (61.71%), Postives = 216/269 (80.30%), Query Frame = 0 

Query: 4   PLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLI 63
           P++ +VC LY+QIS L +L PSK+V+ LFT LV TC PP P +D++ L Q +Q +R+HLI
Sbjct: 8   PVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP-IDVSKLCQKIQEIRSHLI 67

Query: 64  RLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSSVA 123
           +LC  AE LLE HFS IL+S+ENP+ +L IFPY+ NY+KLSLLE++ILT +   +P  +A
Sbjct: 68  KLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIA 127

Query: 124 FVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVME 183
           F+GSGPLPL+S+V+A+ HLK T FHN+DID  AN MA  LV +D D+S RM FHT DVM+
Sbjct: 128 FIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMD 187

Query: 184 VTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVR 243
           VT A  +Y+VVFLAALVG+++E+K +V+ HL K+M+PGA LMLRSAHGARAFLYPV+D R
Sbjct: 188 VTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPR 247

Query: 244 DVEASGFEILSVFHPTDEVINSVVIARKV 273
           D+   GFE+LSV+HPTDEVINSV+IARK+
Sbjct: 248 DLR--GFEVLSVYHPTDEVINSVIIARKL 273

[Close]
BLAST of CmoCh05G010850 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.7e-91
Identity = 175/311 (56.27%), Postives = 239/311 (76.85%), Query Frame = 0 

Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           L+ ++C LY++IS L +LKP ++VDTLF QLV TC PP P +D+  + + +Q MR++LI+
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 65  LCATAEALLEVHFSSILASFE-NPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR-VPSSV 124
           +C  AE  LE HFSSIL SFE NP+H+L++FPYY+NYLKLS LEFD+L  +    VP +V
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFVPRTV 128

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVM 184
           AF+GSGPLPL+S+V+AS+HLK +IFHNFDIDP AN +A +LV SD DLS+RM FHT D+M
Sbjct: 129 AFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIM 188

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
           +VTE+   ++VVFLAALVG++++EK +V+ HL KHM+PGA LMLRSAHG RAFLYP+V+ 
Sbjct: 189 DVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP 248

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARKV----SNLVPDN-DNYNDNDTDDEKRAVLVN 304
            D+E  GFE+LSV+HPTDEVINS+VI+RK+    + +V D+ D  +D   +  K  V++N
Sbjct: 249 CDLE--GFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASDLACNCSKIHVIMN 308

Query: 305 TPIILAEKCSG 309
               + E+ +G
Sbjct: 309 KKKSIIEEFAG 317

[Close]
BLAST of CmoCh05G010850 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.8e-88
Identity = 165/269 (61.34%), Postives = 214/269 (79.55%), Query Frame = 0 

Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           L++ +C LY++IS L SLKPS++V+ LF QLV TC PP P +D+  +   +Q +R +LI+
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNLIK 67

Query: 65  LCATAEALLEVHFSSILASF-ENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR-VPSSV 124
           +C  AE  LE HFSSIL S+ +NP+H+L+IFPYY+NYLKL  LEFD+L  +    VP SV
Sbjct: 68  ICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFVPKSV 127

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVM 184
           AF+GSGPLPL+SIV+AS HLK TIFHNFDIDP ANS+A  LV SD D+S+RM FHT D+M
Sbjct: 128 AFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIM 187

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
           +VTE+   ++VVFLAALVG+ +EEK +V+ HL KHMAPGA LMLRSAHG RAFLYP+V+ 
Sbjct: 188 DVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEP 247

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARK 272
            D++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 248 CDLQ--GFEVLSIYHPTDDVINSVVISKK 274

[Close]
BLAST of CmoCh05G010850 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.0e-87
Identity = 161/271 (59.41%), Postives = 208/271 (76.75%), Query Frame = 0 

Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           +++++  LYDQIS L SLKPSKNVDTLF QLV TC P    +D+ ++ + ++ MRA+LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 65  LCATAEALLEVHFSSILASF---ENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSS 124
           LC  AE  LE HFS+IL S    +NP+ +L IFPYYSNYLKL  LEFD+L+ H   VP+ 
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 125 VAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDV 184
           +AFVGSGP+PL+SIV+A  HL  T FHNFDID  AN++A  LV  D DLSKRM+FHTTDV
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 185 MEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVD 244
           +  TE   +Y+VVFLAALVG+++E K + + HL KHMAPGA LMLRSAH  RAFLYP+VD
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 245 VRDVEASGFEILSVFHPTDEVINSVVIARKV 273
             D++  GF++L+++HPTD+V+NSVVIARK+
Sbjct: 248 SSDLK--GFQLLTIYHPTDDVVNSVVIARKL 276

[Close]
BLAST of CmoCh05G010850 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.0e-87
Identity = 161/270 (59.63%), Postives = 211/270 (78.15%), Query Frame = 0 

Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASL-PQPLQAMRAHLI 64
           +++++  LY+QIS+L SLKPSKNVDTLF QLV TC P    +D+  +  + ++ MR+HLI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 65  RLCATAEALLEVHFSSILASFE-NPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSSV 124
           +LC  AE  LE HFS+IL SFE NP+++L IFPYY+NYLKL  LEFD+L+ H   VP+ V
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVM 184
           AF+GSGP+PL+SIV+A  HL  T FHNFDID  AN++A  LV  DSDLSKRM+FHTTDV+
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
              E   +Y+VVFLAALVG+++E K + + HL KHMAPGA +MLRSAHG RAFLYP+VD 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARKV 273
            D++  GFE+L+++HP+D+V+NSVVIARK+
Sbjct: 248 CDLK--GFEVLTIYHPSDDVVNSVVIARKL 275

[Close]
BLAST of CmoCh05G010850 vs. ExPASy TrEMBL
Match: A0A6J1G3L4 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 1.4e-179
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0 

Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180
           SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD
Sbjct: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 327
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]
BLAST of CmoCh05G010850 vs. ExPASy TrEMBL
Match: A0A6J1KIA7 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.2e-169
Identity = 308/326 (94.48%), Postives = 317/326 (97.24%), Query Frame = 0 

Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 31  MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 90

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDIL SHCRRVPS
Sbjct: 91  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILISHCRRVPS 150

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180
           S+AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDP ANSMA +L+ SDSDLSKRMVFHT D
Sbjct: 151 SIAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANSMARRLIRSDSDLSKRMVFHTAD 210

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVT+AFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 211 VMEVTKAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 270

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300
           DV D+EASGFEILSVFHPTDEVINSVVIARKVS+LV DN+NYNDN+TDDEKR VLVNTPI
Sbjct: 271 DVSDIEASGFEILSVFHPTDEVINSVVIARKVSDLVLDNNNYNDNNTDDEKRGVLVNTPI 330

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 327
           ILAEKCSGFKDFGPMI+EL IEEQLS
Sbjct: 331 ILAEKCSGFKDFGPMIDELGIEEQLS 356

[Close]
BLAST of CmoCh05G010850 vs. ExPASy TrEMBL
Match: A0A0A0LGI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.2e-127
Identity = 250/322 (77.64%), Postives = 275/322 (85.40%), Query Frame = 0 

Query: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCS---PPAPGLDIASLPQPLQAM 61
           E  LL +VCALY+QISSL+SLKP KNVDTLFTQLVLTCS   PP  G DIASL QPL+AM
Sbjct: 8   EALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRAM 67

Query: 62  RAHLIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR 121
           RAHLI+LCA AEALLE+HFSS+LA SF +PI NLSIFPYYSNYLKLSLLEFDIL SH RR
Sbjct: 68  RAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRR 127

Query: 122 VPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFH 181
           +P  VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+FH
Sbjct: 128 IPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 187

Query: 182 TTDVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLY 241
           T DVMEVT+   +YEVVFLAALVGL EEEKGRVL+HLGKHMA G+YLMLRSAHGARAFLY
Sbjct: 188 TKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 247

Query: 242 PVVDVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVN 301
           PVVD+  VEASGFEILSVFHPTDEVINSVVIARK   ++ D  +  D D +DEK+ +LVN
Sbjct: 248 PVVDICTVEASGFEILSVFHPTDEVINSVVIARK--KVMFDQSSDEDED-EDEKQGILVN 307

Query: 302 T-PIILAEKCSGFKDFGPMIEE 319
           T  I+LAEKCSGF  F PMIEE
Sbjct: 308 TSSIVLAEKCSGFNGFTPMIEE 326

[Close]
BLAST of CmoCh05G010850 vs. ExPASy TrEMBL
Match: A0A5D3BBH0 (Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001110 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 4.6e-127
Identity = 251/326 (76.99%), Postives = 278/326 (85.28%), Query Frame = 0 

Query: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCS---PPAP-GLDIASLPQPLQA 61
           E  LL++VCALY+QISSL+SLKP KNVDTLFTQLVLTCS   P AP G D+ASL QPLQA
Sbjct: 12  EALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQA 71

Query: 62  MRAHLIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCR 121
           MRAHLI+LCA AEALLE+HFSS+LA SF +PI NLSIFPYYSNYLKLSLLEFDIL SH R
Sbjct: 72  MRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSR 131

Query: 122 RVPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVF 181
           R+P+ VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+F
Sbjct: 132 RLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIF 191

Query: 182 HTTDVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFL 241
           HT DVMEVT+   +YEVVFLAALVGLEEEEKGRVL+HLGKHMA G+YLMLRSAHGARAFL
Sbjct: 192 HTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFL 251

Query: 242 YPVVDVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTD---DEKRA 301
           YPVVD+  VEASGFEILSVFHPTDEVINSVVIARK   +V D  +  D D D   +EK  
Sbjct: 252 YPVVDICTVEASGFEILSVFHPTDEVINSVVIARK--KVVFDQSSVEDEDEDEEEEEKPG 311

Query: 302 VLVNT-PIILAEKCSGFKDFGPMIEE 319
           +LV+T  I+LAEKCSGF  F P+IEE
Sbjct: 312 ILVDTSSIVLAEKCSGFNGFTPIIEE 335

[Close]
BLAST of CmoCh05G010850 vs. ExPASy TrEMBL
Match: A0A1S3BXI8 (probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 2.0e-117
Identity = 227/275 (82.55%), Postives = 247/275 (89.82%), Query Frame = 0 

Query: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCS---PPAP-GLDIASLPQPLQA 61
           E  LL++VCALY+QISSL+SLKP KNVDTLFTQLVLTCS   P AP G D+ASL QPLQA
Sbjct: 12  EALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQA 71

Query: 62  MRAHLIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCR 121
           MRAHLI+LCA AEALLE+HFSS+LA SF +PI NLSIFPYYSNYLKLSLLEFDIL SH R
Sbjct: 72  MRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSR 131

Query: 122 RVPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVF 181
           R+P+ VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+F
Sbjct: 132 RLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIF 191

Query: 182 HTTDVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFL 241
           HT DVMEVT+   +YEVVFLAALVGLEEEEKGRVL+HLGKHMA G+YLMLRSAHGARAFL
Sbjct: 192 HTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFL 251

Query: 242 YPVVDVRDVEASGFEILSVFHPTDEVINSVVIARK 272
           YPVVD+  VEASGFEILSVFHPTDEVINSVVIARK
Sbjct: 252 YPVVDICTVEASGFEILSVFHPTDEVINSVVIARK 286

[Close]
BLAST of CmoCh05G010850 vs. NCBI nr
Match: XP_022946411.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 638.6 bits (1646), Expect = 2.8e-179
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0 

Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180
           SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD
Sbjct: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 327
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]
BLAST of CmoCh05G010850 vs. NCBI nr
Match: KAG6599185.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 629.4 bits (1622), Expect = 1.7e-176
Identity = 320/326 (98.16%), Postives = 324/326 (99.39%), Query Frame = 0 

Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180
           SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAG+LVCSDSDLSKRMVFHT D
Sbjct: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKVS+L+PDNDNYN+NDTDDEKR VLVNTPI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 327
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]
BLAST of CmoCh05G010850 vs. NCBI nr
Match: KAG7030119.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629.0 bits (1621), Expect = 2.2e-176
Identity = 319/326 (97.85%), Postives = 324/326 (99.39%), Query Frame = 0 

Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180
           S+AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAG+LVCSDSDLSKRMVFHT D
Sbjct: 121 SIAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKVS+L+PDNDNYN+NDTDDEKR VLVNTPI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 327
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]
BLAST of CmoCh05G010850 vs. NCBI nr
Match: XP_023545298.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629.0 bits (1621), Expect = 2.2e-176
Identity = 320/326 (98.16%), Postives = 323/326 (99.08%), Query Frame = 0 

Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180
           SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDP AN+MAGQLVCSDSDLSKRMVFHT D
Sbjct: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANTMAGQLVCSDSDLSKRMVFHTAD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKV++LVPDNDNYNDNDTDDEKRAVLVN PI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVTDLVPDNDNYNDNDTDDEKRAVLVNAPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 327
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]
BLAST of CmoCh05G010850 vs. NCBI nr
Match: XP_022998973.1 (nicotianamine synthase-like, partial [Cucurbita maxima])

HSP 1 Score: 604.7 bits (1558), Expect = 4.5e-169
Identity = 308/326 (94.48%), Postives = 317/326 (97.24%), Query Frame = 0 

Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 31  MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 90

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDIL SHCRRVPS
Sbjct: 91  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILISHCRRVPS 150

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180
           S+AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDP ANSMA +L+ SDSDLSKRMVFHT D
Sbjct: 151 SIAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANSMARRLIRSDSDLSKRMVFHTAD 210

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVT+AFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 211 VMEVTKAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 270

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300
           DV D+EASGFEILSVFHPTDEVINSVVIARKVS+LV DN+NYNDN+TDDEKR VLVNTPI
Sbjct: 271 DVSDIEASGFEILSVFHPTDEVINSVVIARKVSDLVLDNNNYNDNNTDDEKRGVLVNTPI 330

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 327
           ILAEKCSGFKDFGPMI+EL IEEQLS
Sbjct: 331 ILAEKCSGFKDFGPMIDELGIEEQLS 356

[Close]
BLAST of CmoCh05G010850 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 335.5 bits (859), Expect = 4.8e-92
Identity = 175/311 (56.27%), Postives = 239/311 (76.85%), Query Frame = 0 

Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           L+ ++C LY++IS L +LKP ++VDTLF QLV TC PP P +D+  + + +Q MR++LI+
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 65  LCATAEALLEVHFSSILASFE-NPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR-VPSSV 124
           +C  AE  LE HFSSIL SFE NP+H+L++FPYY+NYLKLS LEFD+L  +    VP +V
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFVPRTV 128

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVM 184
           AF+GSGPLPL+S+V+AS+HLK +IFHNFDIDP AN +A +LV SD DLS+RM FHT D+M
Sbjct: 129 AFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIM 188

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
           +VTE+   ++VVFLAALVG++++EK +V+ HL KHM+PGA LMLRSAHG RAFLYP+V+ 
Sbjct: 189 DVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP 248

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARKV----SNLVPDN-DNYNDNDTDDEKRAVLVN 304
            D+E  GFE+LSV+HPTDEVINS+VI+RK+    + +V D+ D  +D   +  K  V++N
Sbjct: 249 CDLE--GFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASDLACNCSKIHVIMN 308

Query: 305 TPIILAEKCSG 309
               + E+ +G
Sbjct: 309 KKKSIIEEFAG 317

[Close]
BLAST of CmoCh05G010850 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 327.4 bits (838), Expect = 1.3e-89
Identity = 165/269 (61.34%), Postives = 214/269 (79.55%), Query Frame = 0 

Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           L++ +C LY++IS L SLKPS++V+ LF QLV TC PP P +D+  +   +Q +R +LI+
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNLIK 67

Query: 65  LCATAEALLEVHFSSILASF-ENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR-VPSSV 124
           +C  AE  LE HFSSIL S+ +NP+H+L+IFPYY+NYLKL  LEFD+L  +    VP SV
Sbjct: 68  ICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFVPKSV 127

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVM 184
           AF+GSGPLPL+SIV+AS HLK TIFHNFDIDP ANS+A  LV SD D+S+RM FHT D+M
Sbjct: 128 AFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIM 187

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
           +VTE+   ++VVFLAALVG+ +EEK +V+ HL KHMAPGA LMLRSAHG RAFLYP+V+ 
Sbjct: 188 DVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEP 247

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARK 272
            D++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 248 CDLQ--GFEVLSIYHPTDDVINSVVISKK 274

[Close]
BLAST of CmoCh05G010850 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-88
Identity = 161/271 (59.41%), Postives = 208/271 (76.75%), Query Frame = 0 

Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           +++++  LYDQIS L SLKPSKNVDTLF QLV TC P    +D+ ++ + ++ MRA+LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 65  LCATAEALLEVHFSSILASF---ENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSS 124
           LC  AE  LE HFS+IL S    +NP+ +L IFPYYSNYLKL  LEFD+L+ H   VP+ 
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 125 VAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDV 184
           +AFVGSGP+PL+SIV+A  HL  T FHNFDID  AN++A  LV  D DLSKRM+FHTTDV
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 185 MEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVD 244
           +  TE   +Y+VVFLAALVG+++E K + + HL KHMAPGA LMLRSAH  RAFLYP+VD
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 245 VRDVEASGFEILSVFHPTDEVINSVVIARKV 273
             D++  GF++L+++HPTD+V+NSVVIARK+
Sbjct: 248 SSDLK--GFQLLTIYHPTDDVVNSVVIARKL 276

[Close]
BLAST of CmoCh05G010850 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-88
Identity = 161/270 (59.63%), Postives = 211/270 (78.15%), Query Frame = 0 

Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASL-PQPLQAMRAHLI 64
           +++++  LY+QIS+L SLKPSKNVDTLF QLV TC P    +D+  +  + ++ MR+HLI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 65  RLCATAEALLEVHFSSILASFE-NPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSSV 124
           +LC  AE  LE HFS+IL SFE NP+++L IFPYY+NYLKL  LEFD+L+ H   VP+ V
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVM 184
           AF+GSGP+PL+SIV+A  HL  T FHNFDID  AN++A  LV  DSDLSKRM+FHTTDV+
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
              E   +Y+VVFLAALVG+++E K + + HL KHMAPGA +MLRSAHG RAFLYP+VD 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARKV 273
            D++  GFE+L+++HP+D+V+NSVVIARK+
Sbjct: 248 CDLK--GFEVLTIYHPSDDVVNSVVIARKL 275

[Close]
BLAST of CmoCh05G010850 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 60.1 bits (144), Expect = 3.9e-09
Identity = 31/59 (52.54%), Postives = 43/59 (72.88%), Query Frame = 0 

Query: 218 MAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVFHP--TDEVINSVVIARKVSN 275
           M PGA LMLRSA G R+FLY  VDV   +  GFE+L ++HP  +D  +NSV++ARK+++
Sbjct: 1   MDPGALLMLRSARGLRSFLY--VDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARKLTD 57

The following BLAST results are available for this feature:
BLAST of CmoCh05G010850 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9XGI75.2e-9161.71Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1[more]
Q9C7X56.7e-9156.27Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S... [more]
O804831.8e-8861.34Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1[more]
Q9FF792.0e-8759.41Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1[more]
Q9FKT92.0e-8759.63Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1[more]
BLAST of CmoCh05G010850 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G3L41.4e-179100.00nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 S... [more]
A0A6J1KIA72.2e-16994.48nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=... [more]
A0A0A0LGI41.2e-12777.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1[more]
A0A5D3BBH04.6e-12776.99Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BXI82.0e-11782.55probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
BLAST of CmoCh05G010850 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022946411.12.8e-179100.00nicotianamine synthase-like [Cucurbita moschata][more]
KAG6599185.11.7e-17698.16Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7030119.12.2e-17697.85Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023545298.12.2e-17698.16nicotianamine synthase-like [Cucurbita pepo subsp. pepo][more]
XP_022998973.14.5e-16994.48nicotianamine synthase-like, partial [Cucurbita maxima][more]
BLAST of CmoCh05G010850 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G56430.14.8e-9256.27nicotianamine synthase 4 [more]
AT1G09240.11.3e-8961.34nicotianamine synthase 3 [more]
AT5G04950.11.4e-8859.41nicotianamine synthase 1 [more]
AT5G56080.11.4e-8859.63nicotianamine synthase 2 [more]
AT4G26483.13.9e-0952.54nicotianamine synthases [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 12..273
e-value: 4.4E-61
score: 208.3
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 5..274
e-value: 5.0E-117
score: 390.2
IPR004298Nicotianamine synthasePANTHERPTHR32266NICOTIANAMINE SYNTHASE 3coord: 4..276
IPR004298Nicotianamine synthasePROSITEPS51142NAScoord: 1..276
score: 55.744511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G010850.1CmoCh05G010850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
molecular_function GO:0030410 nicotianamine synthase activity