CmoCh05G009630 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G009630
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.6-like
LocationCmo_Chr05: 7740686 .. 7744184 (+)
RNA-Seq ExpressionCmoCh05G009630
SyntenyCmoCh05G009630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCTCAGCCACCCAACAAAGACCCAAATTTTGAGTCAAATCGCCTCCATCCATTCACAAAAATGGTGGGTTTCTCCAAATTTCCTTCTTTTCAACCTTTGATCCTTCTATAATGTTTCTTGGATGGTTTGTTATAGGAAGTTGTTAAGGAGGAGGGGGACGGCGGCGTATGGGAAGGCTACGTCGACTGGCGGAGGAGGCCGGCCGTGAGAGGGCGGCACGGTGGCATGAGGGCGGCTGGGTTTGTGTTAGGTTAGTGAACTCTTCGAATCTCCTTTTTGTTTTTGTCATACATGTCAATTAACACTGAGTTGTCAATTAACACTGAGTTATATTAGGAGTGGAAGTACTTGAGAATTTAGCGTTTCTAGCTAATGCAAGCAACTTGGTGATGTTTTTGAGGAAGTACATGCAATTTTCACCGACGGAATCCGCTAACCATGTCACCAATTTCATGGGCACTGCCTTCCTTCTCGCCCTTCTTGGCGGCTTCCTTTCCGATGCCTTTTTCACCACTTACTACGTCTTCTTATTCTCCTCTCTTCTCGAGTTTCTGGTTCGTACTCTTACTCTCCTCTCAATATTAAGTTGTATTTAAATGTGTGTAATTTTTTTTTATTTAAAAGTTAAATTTTATAAAGAAAGTGAGAAAAATAATAATTTTTGCTTTGTTTTTATTGAGTTGGAAAATGAAATGAATTGAATGAAAATAATGTCCCCACAGCCAAAATAACGTATGTACCCGTTTCTTTCCAAGATAATGACACACATCCCAACAAACCCATCTTTATAATAATTAAAAAAAAAACAAATTTCATAATTTAAGACCTAATACGACCACCGTCGTCCTCACGATACGTTCAAAATTCGAAAATAGGTCAACCCAATTAAGGTAATTGTTAAAAAAAAGGTCGTAGGTTCAAATTTTCTTTCACCGACTTATAAGGCGGAGGGAAATTAATTAAAATTTTTATGATTACGATGAATAATAAAAAAAAAATATAGTATTCCCTCAGCTTTTACAAATTGCATGACGTATAATTTGGATGGAGGAAAATGATAAATAAATAAATAAATAATTTGGTGTGAAAAGACATGAGGGGAGAGTGTGTTGTTGACGACATAAAAGGGTTGTTTTTTTTTTGGAATTGGGTGTAAAAGGGGTTGGTGATTCTCACGGTGGAAGCGAAGGCGGCAACGGCGGCATCGGCGGCGGTGGTGTTCGTGGGGCTATACCTGGTGGCGGTGGGGGTGGGGGGGATAAAGGGGTCGTTGCCGACGCATGGGGCGGAGCAGTTCGAGGAGGGGACGGCGGAGGGGAGGAGGGGGAGGTCGAGTTTCTTCAACTACTTCGTGTTTAGTTTGTCATGTGGGGCGGTTGTGGCGGTGACGTTGGTGGTGTGGATGGAAGACAACTTGGGGTGGGAGTGGGGGTTTGGAATCTCAACCATCGCTATTTTGGTGTCGATTCCTTTGTTCCTTGCTGGTTCCTGTTTTTATCGTAATAAGATCCCCGCTGGTGCTCCTCTTACCACCATTCTCAAGGTACTTCTCTTTCATTAAACTTTCTTTTTTTTTTTTTTTTCATTTTCATCTCTTTTTTTTTTTTATCTAAATTTTTGTTTATAAAAATGTACCCACCATGAAAGTCCCACACGTCCGCTAATTTACGGAATGATCGTGGTTTTATAACCAAGGAATACTCGCTTTTTGGGGAAGTCCAAGTAAAGCCACGAGAGCTTATGCTCAAAGTGGACAATATCGTACACAATGCTAATTTACGGAATGATCGTGGTTTTATAACCAAGGAATACTCGCTTTTTGGGGAAGTCCAAGTAAAGCCACGAGAGCTTATGCTCAAAGTGGACAATATCGTACCATTGTGAGAGTTGTATTCATCTCACATCTATGAATTTAAAGTAATGGGTTCTCATCGAACGGTAAAATTTTATAAAAAATGATAAAAAAAACTTTTAATATTCTTAATTTTTTTTAAAAACTTTTAAAATTATGTCTATATGCTATTAAAGGTATTTTTGGAATTTCTTAAAAGATTCCAAAGTTATTTTTTAAATTGAAGGGTATTATTAAAACTTTACAGAAATGAAAAAGGTTGATTTTGATGATCGGGTATCCGTATTCTTTTTTAAAATTTAAGACTAACATTTGTACTCTTAAATTTTTTTATTAAAGTTTTTATGTTATTAACAATTTCTTAAAAAATTTGAAAACTTAAGAGTATTATTACAAATTTATAAAAATGATTTTGATGATCCGATATTCATATTATTTTTTAAAATTTTGGAGTAACGCACCAACGTTAAGACTCTTAAATTTCTTTAAAGTTTTTATGTTATTAACAAAAGATCACGAGGGAATTATTGAAACAGAAAGATTTTTATTTTTTAAAATTTAAAAGTATTATTGCATTCAATGTTGATAAAGAAATGTTTGGGTTGGATGATTCAGGTATTGGTAGCAGCAACGTTGAACGGTAGAAGCAGAAACAAAACAGCATCAAACGCAGTAGCAAGCATGACAAGAAGCCCATCCACCACAACCCCAAAGCCAACCAAGGAGCCAACACACACCACACCACCACCAACCCAAACCCTCAAGTTCCTAAACAAAGCAATACAAACCCCACCCTTCCACCCATCACTAAGCTGCACAACCCAACAGCTAGAAGACGTCAAAGTAGTCTTAAAACTCCTCCCAATCTTCGCATGCACCATAATCCTCAACTCCTGCCTCGCTCAACTCTCCACCTTCTCCGTCGAACAAGCCTCCACTATGAACACCAAAATCGGATCCATCAAACTCCCACCCGCCTCTCTCCCTATCTTCCCTATACTCTTCATCATACTCCTCGCCCCGCTCTACAACCATCTCATTATCCCCTTCGCTCGCACTCTCACTCGAACCGAATCCGGTATCACTCACCTTCAACGCATTGGCGTCGGCCTACTTCTCTCAATCGTCGCCATGGCCATCGCTGCCCTCATCGAGGTCAAGCGCAAGGCTATTGCGGACGCACACCCCGCGGCCCACCCGCTCCCCATCACATTCCTTTGGATTGGCTTCCAGTACTTGTTTTTGGGGTCTGCTGATTTGTTCACCTTGGCGGGGTTGTTGGAGTTTTTCTTTACCGAGGCTCCTTCGACCATGAGGTCTTTGGCTACGGCTCTCTCTTGGGCTTCATTGGCGGTTGGCTACTATCTAAGCTCCGTTATTGTGTCCCTTGTCAACCGTGTCACTGCCAAGTCTTCCCACTCGCCTTGGCTGTCTGCCCAAGACATCAATCACTATGATCTTCATAAGTTCTACTGGCTCATGTGTGCGCTTAGTGCATTGAATTTCTTGCATTTCCTTTTTTGGGCCATCAAGTATAAATATCGGTCCACCCTAGCGAGCAAGTGATATGCTACGATGATATGTTATATTATTGTATCTTTCTCGATACTTAAGCGTTCAATATATTATATACAGATATAAACGCG

mRNA sequence

AGCTCAGCCACCCAACAAAGACCCAAATTTTGAGTCAAATCGCCTCCATCCATTCACAAAAATGGAAGTTGTTAAGGAGGAGGGGGACGGCGGCGTATGGGAAGGCTACGTCGACTGGCGGAGGAGGCCGGCCGTGAGAGGGCGGCACGGTGGCATGAGGGCGGCTGGGTTTGTGTTAGGAGTGGAAGTACTTGAGAATTTAGCGTTTCTAGCTAATGCAAGCAACTTGGTGATGTTTTTGAGGAAGTACATGCAATTTTCACCGACGGAATCCGCTAACCATGTCACCAATTTCATGGGCACTGCCTTCCTTCTCGCCCTTCTTGGCGGCTTCCTTTCCGATGCCTTTTTCACCACTTACTACGTCTTCTTATTCTCCTCTCTTCTCGAGTTTCTGGGGTTGGTGATTCTCACGGTGGAAGCGAAGGCGGCAACGGCGGCATCGGCGGCGGTGGTGTTCGTGGGGCTATACCTGGTGGCGGTGGGGGTGGGGGGGATAAAGGGGTCGTTGCCGACGCATGGGGCGGAGCAGTTCGAGGAGGGGACGGCGGAGGGGAGGAGGGGGAGGTCGAGTTTCTTCAACTACTTCGTGTTTAGTTTGTCATGTGGGGCGGTTGTGGCGGTGACGTTGGTGGTGTGGATGGAAGACAACTTGGGGTGGGAGTGGGGGTTTGGAATCTCAACCATCGCTATTTTGGTGTCGATTCCTTTGTTCCTTGCTGGTTCCTGTTTTTATCGTAATAAGATCCCCGCTGGTGCTCCTCTTACCACCATTCTCAAGGTATTGGTAGCAGCAACGTTGAACGGTAGAAGCAGAAACAAAACAGCATCAAACGCAGTAGCAAGCATGACAAGAAGCCCATCCACCACAACCCCAAAGCCAACCAAGGAGCCAACACACACCACACCACCACCAACCCAAACCCTCAAGTTCCTAAACAAAGCAATACAAACCCCACCCTTCCACCCATCACTAAGCTGCACAACCCAACAGCTAGAAGACGTCAAAGTAGTCTTAAAACTCCTCCCAATCTTCGCATGCACCATAATCCTCAACTCCTGCCTCGCTCAACTCTCCACCTTCTCCGTCGAACAAGCCTCCACTATGAACACCAAAATCGGATCCATCAAACTCCCACCCGCCTCTCTCCCTATCTTCCCTATACTCTTCATCATACTCCTCGCCCCGCTCTACAACCATCTCATTATCCCCTTCGCTCGCACTCTCACTCGAACCGAATCCGGTATCACTCACCTTCAACGCATTGGCGTCGGCCTACTTCTCTCAATCGTCGCCATGGCCATCGCTGCCCTCATCGAGGTCAAGCGCAAGGCTATTGCGGACGCACACCCCGCGGCCCACCCGCTCCCCATCACATTCCTTTGGATTGGCTTCCAGTACTTGTTTTTGGGGTCTGCTGATTTGTTCACCTTGGCGGGGTTGTTGGAGTTTTTCTTTACCGAGGCTCCTTCGACCATGAGGTCTTTGGCTACGGCTCTCTCTTGGGCTTCATTGGCGGTTGGCTACTATCTAAGCTCCGTTATTGTGTCCCTTGTCAACCGTGTCACTGCCAAGTCTTCCCACTCGCCTTGGCTGTCTGCCCAAGACATCAATCACTATGATCTTCATAAGTTCTACTGGCTCATGTGTGCGCTTAGTGCATTGAATTTCTTGCATTTCCTTTTTTGGGCCATCAAGTATAAATATCGGTCCACCCTAGCGAGCAAGTGATATGCTACGATGATATGTTATATTATTGTATCTTTCTCGATACTTAAGCGTTCAATATATTATATACAGATATAAACGCG

Coding sequence (CDS)

ATGGAAGTTGTTAAGGAGGAGGGGGACGGCGGCGTATGGGAAGGCTACGTCGACTGGCGGAGGAGGCCGGCCGTGAGAGGGCGGCACGGTGGCATGAGGGCGGCTGGGTTTGTGTTAGGAGTGGAAGTACTTGAGAATTTAGCGTTTCTAGCTAATGCAAGCAACTTGGTGATGTTTTTGAGGAAGTACATGCAATTTTCACCGACGGAATCCGCTAACCATGTCACCAATTTCATGGGCACTGCCTTCCTTCTCGCCCTTCTTGGCGGCTTCCTTTCCGATGCCTTTTTCACCACTTACTACGTCTTCTTATTCTCCTCTCTTCTCGAGTTTCTGGGGTTGGTGATTCTCACGGTGGAAGCGAAGGCGGCAACGGCGGCATCGGCGGCGGTGGTGTTCGTGGGGCTATACCTGGTGGCGGTGGGGGTGGGGGGGATAAAGGGGTCGTTGCCGACGCATGGGGCGGAGCAGTTCGAGGAGGGGACGGCGGAGGGGAGGAGGGGGAGGTCGAGTTTCTTCAACTACTTCGTGTTTAGTTTGTCATGTGGGGCGGTTGTGGCGGTGACGTTGGTGGTGTGGATGGAAGACAACTTGGGGTGGGAGTGGGGGTTTGGAATCTCAACCATCGCTATTTTGGTGTCGATTCCTTTGTTCCTTGCTGGTTCCTGTTTTTATCGTAATAAGATCCCCGCTGGTGCTCCTCTTACCACCATTCTCAAGGTATTGGTAGCAGCAACGTTGAACGGTAGAAGCAGAAACAAAACAGCATCAAACGCAGTAGCAAGCATGACAAGAAGCCCATCCACCACAACCCCAAAGCCAACCAAGGAGCCAACACACACCACACCACCACCAACCCAAACCCTCAAGTTCCTAAACAAAGCAATACAAACCCCACCCTTCCACCCATCACTAAGCTGCACAACCCAACAGCTAGAAGACGTCAAAGTAGTCTTAAAACTCCTCCCAATCTTCGCATGCACCATAATCCTCAACTCCTGCCTCGCTCAACTCTCCACCTTCTCCGTCGAACAAGCCTCCACTATGAACACCAAAATCGGATCCATCAAACTCCCACCCGCCTCTCTCCCTATCTTCCCTATACTCTTCATCATACTCCTCGCCCCGCTCTACAACCATCTCATTATCCCCTTCGCTCGCACTCTCACTCGAACCGAATCCGGTATCACTCACCTTCAACGCATTGGCGTCGGCCTACTTCTCTCAATCGTCGCCATGGCCATCGCTGCCCTCATCGAGGTCAAGCGCAAGGCTATTGCGGACGCACACCCCGCGGCCCACCCGCTCCCCATCACATTCCTTTGGATTGGCTTCCAGTACTTGTTTTTGGGGTCTGCTGATTTGTTCACCTTGGCGGGGTTGTTGGAGTTTTTCTTTACCGAGGCTCCTTCGACCATGAGGTCTTTGGCTACGGCTCTCTCTTGGGCTTCATTGGCGGTTGGCTACTATCTAAGCTCCGTTATTGTGTCCCTTGTCAACCGTGTCACTGCCAAGTCTTCCCACTCGCCTTGGCTGTCTGCCCAAGACATCAATCACTATGATCTTCATAAGTTCTACTGGCTCATGTGTGCGCTTAGTGCATTGAATTTCTTGCATTTCCTTTTTTGGGCCATCAAGTATAAATATCGGTCCACCCTAGCGAGCAAGTGA

Protein sequence

MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK
Homology
BLAST of CmoCh05G009630 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 6.1e-201
Identity = 377/575 (65.57%), Postives = 458/575 (79.65%), Query Frame = 0

Query: 13  WEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKYMQFSPTESA 72
           WEGY DWR R AV+GRHGGM AA FVL VE+LENLA+LANASNLV++LR+YM  SP++SA
Sbjct: 10  WEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSA 69

Query: 73  NHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEAKA--------- 132
           N VTNFMGTAFLLALLGGFLSDAFF+T+ +FL S+ +EFLGL+ILT++A+          
Sbjct: 70  NDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCD 129

Query: 133 ------ATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFV 192
                  + + AA++FVGLYLVA+GVGGIKGSL +HGAEQF+E T +GR+ RS+FFNYFV
Sbjct: 130 SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFV 189

Query: 193 FSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTT 252
           F L+CGA+VAVT VVW+EDN GWEWGFG+STIAI VSI +FL+GS FYRNKIP G+PLTT
Sbjct: 190 FCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTT 249

Query: 253 ILKVLVAATLNGRSRNKTASNAVASMTRSPST--------------TTPKPTKEPTHTTP 312
           ILKVL+AA++   S + ++SNAVASM+ SPS                  KP +E     P
Sbjct: 250 ILKVLLAASVKCCS-SGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQE--EALP 309

Query: 313 PP---TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLST 372
           P    T +LK LN A    P H  L CT QQ+EDVK+VLK+LPIFACTI+LN CLAQLST
Sbjct: 310 PRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLST 369

Query: 373 FSVEQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQ 432
           FSV+QA++MNTKIGS+K+PPASLPIFP++FI++LAP+Y+HLIIPFAR  T+TE+G+THLQ
Sbjct: 370 FSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQ 429

Query: 433 RIGVGLLLSIVAMAIAALIEVKRKAIADAH---PAAHPLPITFLWIGFQYLFLGSADLFT 492
           RIGVGL+LSI+AMA+AAL+E+KRK +A       +   LP+TFLWI  QYLFLGSADLFT
Sbjct: 430 RIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFT 489

Query: 493 LAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDI 552
           LAGLLE+FFTEAPS+MRSLAT+LSWASLA+GYYLSSVIVS+VN +T  S ++PWL  + I
Sbjct: 490 LAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSI 549

BLAST of CmoCh05G009630 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 5.2e-184
Identity = 350/570 (61.40%), Postives = 436/570 (76.49%), Query Frame = 0

Query: 4   VKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKY 63
           V+  GD   WEGY DWR + A+RGRHGGM AA FVL VE+LENLAFLANASNLV++L+ +
Sbjct: 3   VEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNF 62

Query: 64  MQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEAKA 123
           M  S   S++ VT FM TAFLLALLGGFL+DAFF+T+ +FL S+ +EFLGL++LT++A+ 
Sbjct: 63  MHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARR 122

Query: 124 AT------------------AASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEG 183
            +                   + AA +FVGLYLV++G+GGIKGSLP+HGAEQF+EGT +G
Sbjct: 123 PSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKG 182

Query: 184 RRGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFY 243
           R+ RS+FFNY+VF LSCGA+VAVT VVW+EDN GWEWGFG+STI+I +SI +FL GS FY
Sbjct: 183 RKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFY 242

Query: 244 RNKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPP 303
           +NKIP G+PLTTI KVL+AA++   S +KT+SN   S        +    K P+ +    
Sbjct: 243 KNKIPRGSPLTTIFKVLLAASIVSCS-SKTSSNHFTSR----EVQSEHEEKTPSQSL--- 302

Query: 304 TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQ 363
           T +L  LNKAI+    H  L CT QQ+EDVK+VLK+LPIF CTI+LN CLAQLST+SV Q
Sbjct: 303 TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQ 362

Query: 364 ASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVG 423
           A+TMN KI +  +P ASLP+FP++F+++LAP Y+HLIIPFAR +T++E GITHLQRIGVG
Sbjct: 363 AATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVG 422

Query: 424 LLLSIVAMAIAALIEVKRKAI---ADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLL 483
           L+LSIVAMA+AAL+E+KRK +   A    +   LPITFLWI  QYLFLGSADLFTLAGLL
Sbjct: 423 LVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLL 482

Query: 484 EFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDL 543
           EFFFTEAPS+MRSLAT+LSWASLA+GYYLSSV+V +VNRVT  +  SPWL  + +N   L
Sbjct: 483 EFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRNRL 542

Query: 544 HKFYWLMCALSALNFLHFLFWAIKYKYRST 553
             FYWLMC LS +NFLH+LFWA +YKY ST
Sbjct: 543 DLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of CmoCh05G009630 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 634.4 bits (1635), Expect = 1.2e-180
Identity = 340/561 (60.61%), Postives = 430/561 (76.65%), Query Frame = 0

Query: 2   EVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLR 61
           E + E      W GYVDWR RPA+RGRHGGM AA FVL VEVLENLAFLANASNLV++L 
Sbjct: 30  EQMDEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLS 89

Query: 62  KYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEA 121
             M FSP+ +AN VT FMGTAF LALLGGFL+DAFFTT++++L S+ +EFLGL++LTV+A
Sbjct: 90  TKMGFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQA 149

Query: 122 KAATAA--SAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFS 181
              +    S   +FVGLYLVA+GVGGIKGSLP HGAEQF+E T+ GRR RS FFNYF+FS
Sbjct: 150 HEHSTEPWSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFS 209

Query: 182 LSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTIL 241
           LSCGA++AVT+VVW+EDN GW +GFG+ST AIL+S+P+FLAGS  YR K+P+G+P+TT+ 
Sbjct: 210 LSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSPITTLF 269

Query: 242 KVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTP 301
           KVL AA      + +T+   V   TR+    +   TK+  +          FL + ++  
Sbjct: 270 KVLTAALYAKYKKRRTSRIVVTCHTRNDCDDS--VTKQ--NCDGDDGFLGSFLGEVVRER 329

Query: 302 PFHP-SLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKL 361
              P  L CT +Q++DVK+V+K+LPIF  TI+LN CLAQLSTFSV+QASTMNTK+GS  +
Sbjct: 330 ESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFSVQQASTMNTKLGSFTV 389

Query: 362 PPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAAL 421
           PPA+LP+FP++F+++LAP YNHL++P AR  T+TE+GITHLQRIG GL+LSIVAMA+AAL
Sbjct: 390 PPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAAL 449

Query: 422 IEVKRKAIA--------DAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAP 481
           +E KRK +          +  ++ PLPITFLW+  QY+FLGSADLFTLAG++EFFFTEAP
Sbjct: 450 VETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEAP 509

Query: 482 STMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMC 541
           STMRSLAT+LSWASLA+GYY SSV+VS VN VT  + H+PWL  +++N Y L +FYWLMC
Sbjct: 510 STMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGENLNQYHLERFYWLMC 569

Query: 542 ALSALNFLHFLFWAIKYKYRS 552
            LS +NFLH+LFWA +Y YRS
Sbjct: 570 VLSGINFLHYLFWASRYVYRS 586

BLAST of CmoCh05G009630 vs. ExPASy Swiss-Prot
Match: Q9LSF0 (Protein NRT1/ PTR FAMILY 4.1 OS=Arabidopsis thaliana OX=3702 GN=NPF4.1 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 2.6e-95
Identity = 224/547 (40.95%), Postives = 338/547 (61.79%), Query Frame = 0

Query: 14  EGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKYMQFSPTESAN 73
           E + DWR + A+ G+HGG++AA     VE +EN+ FLA ++N +M+  K M +S  ++A 
Sbjct: 7   EKFEDWRGKEAISGKHGGIKAAFIACVVETMENMVFLACSTNFMMYFTKSMNYSTPKAAT 66

Query: 74  HVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEA----------KA 133
            VTNF+GT+FLL + GGF++D+F T +  F+    +E LGL++LT++A          K 
Sbjct: 67  MVTNFVGTSFLLTIFGGFVADSFLTRFAAFVLFGSIELLGLIMLTLQAHITKLQPQGGKK 126

Query: 134 ATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSLSCG 193
            +   + V+F GLY +A+GVGG+KGSLP HG +Q   GT   +R  S FFN++ FS+  G
Sbjct: 127 PSTPQSTVLFTGLYAIAIGVGGVKGSLPAHGGDQI--GT-RNQRLISGFFNWYFFSVCLG 186

Query: 194 AVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILKVLV 253
             +AVTL+VW+E+N+GW   F IST  +  +I +F+AG   YR K PAG+PLT I+ V V
Sbjct: 187 GFLAVTLMVWIEENIGWSSSFTISTAVLASAIFVFVAGCPMYRFKRPAGSPLTRIVNVFV 246

Query: 254 AATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPPFHP 313
           +A    R+RN+  ++A   +T++ ++T     K   H         KFLNKA      + 
Sbjct: 247 SA---ARNRNRFVTDAEV-VTQNHNST----DKSIHH------NKFKFLNKA----KLNN 306

Query: 314 SLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIG-SIKLPPAS 373
            +S T  Q+E+ +  L LLPIF  TII+N C+AQ+ TFSV+Q    N K+  S ++P AS
Sbjct: 307 KISAT--QVEETRTFLALLPIFGSTIIMNCCVAQMGTFSVQQGMVTNRKLSRSFEIPVAS 366

Query: 374 LPIFPILFIILLAPLYNHLIIPFARTLTRTE-SGITHLQRIGVGLLLSIVAMAIAALIEV 433
           L   P+L ++    LY    +   R L+ +E S   +L+RIG GL L+ ++MA+AA++EV
Sbjct: 367 LNAIPLLCMLSSLALYE---LFGKRILSNSERSSSFNLKRIGYGLALTSISMAVAAIVEV 426

Query: 434 KRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL 493
           KRK     H A H  + I+  W+  Q++ L  +D+ T+ G+LEFFF E+P++MRS++TAL
Sbjct: 427 KRK-----HEAVHNNIKISVFWLELQFVMLSLSDMLTVGGMLEFFFRESPASMRSMSTAL 486

Query: 494 SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF 548
            W S A+G++LSSV+V +VN +T       WL   D+N   L  FY ++C L+ LN  ++
Sbjct: 487 GWCSTAMGFFLSSVLVEVVNGITG------WL-RDDLNESRLELFYLVLCVLNTLNLFNY 515

BLAST of CmoCh05G009630 vs. ExPASy Swiss-Prot
Match: Q9LSE8 (Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.0e-94
Identity = 214/549 (38.98%), Postives = 328/549 (59.74%), Query Frame = 0

Query: 14  EGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKYMQFSPTESAN 73
           E + DW+ + A+ G+HGG+RAA  V  V ++EN+ F+AN  N V +    M ++P  +AN
Sbjct: 7   EKFEDWKGKEAIPGKHGGIRAASIVCVVVMMENIVFIANGFNFVKYFMGSMHYTPATAAN 66

Query: 74  HVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEA----------KA 133
            VTNFMGT+FLL L GGF++D+F T +  F+    +E +GL++LT +A          K 
Sbjct: 67  MVTNFMGTSFLLTLFGGFIADSFVTHFTTFIVFCCIELMGLILLTFQAHNPKLLPEKDKT 126

Query: 134 ATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSLSCG 193
            +   +A++F GLY +A+G GG+K SLP+HG +Q +       R  S FF++  FS+  G
Sbjct: 127 PSTLQSAILFTGLYAMAIGTGGLKASLPSHGGDQIDR---RNPRLISRFFDWLYFSICSG 186

Query: 194 AVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILKVLV 253
            ++AVT+V+W+E+  GW W F IS   +  ++ +F  G  FYR K P G+PL  I  V++
Sbjct: 187 CLLAVTVVLWIEEKKGWIWSFNISVGILATALCIFTVGLPFYRFKRPNGSPLKKIAIVII 246

Query: 254 AATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPPFHP 313
           +A     +RN+  S+    M R   +      K  +H        LK+++KA      + 
Sbjct: 247 SA-----ARNRNKSDLDEEMMRGLISI----YKNNSH------NKLKWIDKA----TLNK 306

Query: 314 SLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKI-GSIKLPPAS 373
           ++S T  ++E+ +  L LLPIF  TI+++ C+AQLSTFS +Q   MN K+  S ++P  S
Sbjct: 307 NISET--EVEETRTFLGLLPIFGSTIVMSCCVAQLSTFSAQQGMLMNKKLFHSFEIPVPS 366

Query: 374 LPIFPILFIILLAPLYNHLIIPFARTLT---RTESGITHLQRIGVGLLLSIVAMAIAALI 433
           L   P++F++L  PLY      F + ++      S   +L+RIG+GL LS V+MA++A++
Sbjct: 367 LTAIPLIFMLLSIPLYEF----FGKKISSGNNNRSSSFNLKRIGLGLALSSVSMAVSAIV 426

Query: 434 EVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLAT 493
           E KRK     H   H    I+ LW+ FQYL L  +D+ TL G+LEFF+ EAPS M+S++T
Sbjct: 427 EAKRK-----HEVVHNNFRISVLWLVFQYLMLSVSDMLTLGGMLEFFYREAPSNMKSIST 486

Query: 494 ALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFL 548
           AL W S A+G++LS+ +V + N VT +  H  WL  +D+N   L  FY L+C L+ LN L
Sbjct: 487 ALGWCSTALGFFLSTTLVEVTNAVTGRLGHQ-WLGGEDLNKTRLELFYVLLCVLNTLNLL 521

BLAST of CmoCh05G009630 vs. ExPASy TrEMBL
Match: A0A6J1G2M6 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111450266 PE=3 SV=1)

HSP 1 Score: 1057.4 bits (2733), Expect = 2.1e-305
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60
           MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL
Sbjct: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60

Query: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120
           RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE
Sbjct: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120

Query: 121 AKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSL 180
           AKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSL
Sbjct: 121 AKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSL 180

Query: 181 SCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILK 240
           SCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILK
Sbjct: 181 SCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILK 240

Query: 241 VLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP 300
           VLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP
Sbjct: 241 VLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP 300

Query: 301 FHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP 360
           FHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP
Sbjct: 301 FHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP 360

Query: 361 ASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE 420
           ASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE
Sbjct: 361 ASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE 420

Query: 421 VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL 480
           VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL
Sbjct: 421 VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL 480

Query: 481 SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF 540
           SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF
Sbjct: 481 SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF 540

Query: 541 LFWAIKYKYRSTLASK 557
           LFWAIKYKYRSTLASK
Sbjct: 541 LFWAIKYKYRSTLASK 556

BLAST of CmoCh05G009630 vs. ExPASy TrEMBL
Match: A0A6J1KG66 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111493955 PE=3 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 4.0e-288
Identity = 524/557 (94.08%), Postives = 539/557 (96.77%), Query Frame = 0

Query: 1   MEVVKEEGD-GGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMF 60
           MEVVKEEGD GGVWEGYVDWRRRPAVRGRHGGMRAA FVLGVEVLENLAFLANASNLVMF
Sbjct: 1   MEVVKEEGDGGGVWEGYVDWRRRPAVRGRHGGMRAAVFVLGVEVLENLAFLANASNLVMF 60

Query: 61  LRKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTV 120
           LRKYMQFSP +SANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTV
Sbjct: 61  LRKYMQFSPAKSANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTV 120

Query: 121 EAKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFS 180
           EAKAA+AASAA VFVGLYLVAVGVGGIKGSL  HGAEQFEEGTAEGRRGRSSFFNYFVFS
Sbjct: 121 EAKAASAASAAAVFVGLYLVAVGVGGIKGSLAAHGAEQFEEGTAEGRRGRSSFFNYFVFS 180

Query: 181 LSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTIL 240
           LSCGA+V VTLVVW+EDNLGWEWGFGISTIAILVSIPLFL+GSCFYRNKIP  APLTTIL
Sbjct: 181 LSCGALVGVTLVVWIEDNLGWEWGFGISTIAILVSIPLFLSGSCFYRNKIPTAAPLTTIL 240

Query: 241 KVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTP 300
           KVLVAATLNGR RNKTA+NAVASMTRSPSTTTPKPTKEPTHTT PPTQTLKFLNKAIQTP
Sbjct: 241 KVLVAATLNGRRRNKTATNAVASMTRSPSTTTPKPTKEPTHTT-PPTQTLKFLNKAIQTP 300

Query: 301 PFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLP 360
           PFHPSLSCTTQQLEDVK+VLKLLPIFACTIILNSCLAQLSTFSVEQASTMNT+IGSIK P
Sbjct: 301 PFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTQIGSIKFP 360

Query: 361 PASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALI 420
           PASLPIFP++FII+LAPLYNHLI+PFARTLTRTESGITHLQRIGVGLLLSIVAM IAALI
Sbjct: 361 PASLPIFPVIFIIILAPLYNHLIVPFARTLTRTESGITHLQRIGVGLLLSIVAMVIAALI 420

Query: 421 EVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATA 480
           EVKRKA+ADAHPAAHPLPITFLWI FQYLFLGSADLFTLAGLLEFFFTEAP TMRSLATA
Sbjct: 421 EVKRKAVADAHPAAHPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPPTMRSLATA 480

Query: 481 LSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLH 540
           LSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLS QDINHYDLHKFYWLMCALSALNFLH
Sbjct: 481 LSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLH 540

Query: 541 FLFWAIKYKYRSTLASK 557
           FLFWA+KYKYRST+  K
Sbjct: 541 FLFWALKYKYRSTIERK 556

BLAST of CmoCh05G009630 vs. ExPASy TrEMBL
Match: A0A5D3BLL0 (Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00950 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 3.5e-244
Identity = 447/574 (77.87%), Postives = 504/574 (87.80%), Query Frame = 0

Query: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60
           ME VKEE DG +WEGYVDWR+RPAV+GRHGGM AAGFVLGVEVLENLAFLANASNLVM+L
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120
           RKYM FSP +SANHVT FMGTAFLLALLGGFLSDAFFTTYYVFLFSS LEFLGLV+LTV+
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQ 120

Query: 121 AKAATAASA-------------AVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRR 180
           AK +++  A             AV+F+GLYLVA+GVGGIKGSLP HGAEQF+E T +GR+
Sbjct: 121 AKVSSSCEAGTKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGRK 180

Query: 181 GRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRN 240
            RSSFFNYFVFSLSCGA+VAVT VVW+EDNLGW+WGFGISTI+  +SIPLF AGS FYRN
Sbjct: 181 RRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYRN 240

Query: 241 KIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTT-TPKP--TKEPTHT-TP 300
           KIP G+PLTTILKVLVAATLN RS  K A+NAVAS+ RSPSTT TPKP   K+ T + T 
Sbjct: 241 KIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDLTESNTT 300

Query: 301 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSV 360
            PTQ+LKFLNKA+Q PPFHPS+ CTTQQLEDVK+V+K+LPIFACTIILNSCLAQLSTFS+
Sbjct: 301 TPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFSI 360

Query: 361 EQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 420
           EQA+TMNTKI S+K+PPASLP+FP+LFII+LAPLY+HLIIPFAR+LTRTE+GITHLQRIG
Sbjct: 361 EQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGITHLQRIG 420

Query: 421 VGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGLL 480
           VGLLLS++AMA+AAL+E KRKA+ADA+P    PLPITFLWI FQYLFLGSADLFTLAGLL
Sbjct: 421 VGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLFTLAGLL 480

Query: 481 EFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDL 540
           EFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VN V+AK+ H PWLS  +INHY L
Sbjct: 481 EFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYRL 540

Query: 541 HKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 557
             FYW+MC LS  NFLH+LFWA+KYKYRST  +K
Sbjct: 541 SNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of CmoCh05G009630 vs. ExPASy TrEMBL
Match: A0A1S3C3A1 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 3.5e-244
Identity = 447/574 (77.87%), Postives = 504/574 (87.80%), Query Frame = 0

Query: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60
           ME VKEE DG +WEGYVDWR+RPAV+GRHGGM AAGFVLGVEVLENLAFLANASNLVM+L
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120
           RKYM FSP +SANHVT FMGTAFLLALLGGFLSDAFFTTYYVFLFSS LEFLGLV+LTV+
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQ 120

Query: 121 AKAATAASA-------------AVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRR 180
           AK +++  A             AV+F+GLYLVA+GVGGIKGSLP HGAEQF+E T +GR+
Sbjct: 121 AKVSSSCEAGTKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGRK 180

Query: 181 GRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRN 240
            RSSFFNYFVFSLSCGA+VAVT VVW+EDNLGW+WGFGISTI+  +SIPLF AGS FYRN
Sbjct: 181 RRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYRN 240

Query: 241 KIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTT-TPKP--TKEPTHT-TP 300
           KIP G+PLTTILKVLVAATLN RS  K A+NAVAS+ RSPSTT TPKP   K+ T + T 
Sbjct: 241 KIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDLTESNTT 300

Query: 301 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSV 360
            PTQ+LKFLNKA+Q PPFHPS+ CTTQQLEDVK+V+K+LPIFACTIILNSCLAQLSTFS+
Sbjct: 301 TPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFSI 360

Query: 361 EQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 420
           EQA+TMNTKI S+K+PPASLP+FP+LFII+LAPLY+HLIIPFAR+LTRTE+GITHLQRIG
Sbjct: 361 EQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGITHLQRIG 420

Query: 421 VGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGLL 480
           VGLLLS++AMA+AAL+E KRKA+ADA+P    PLPITFLWI FQYLFLGSADLFTLAGLL
Sbjct: 421 VGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLFTLAGLL 480

Query: 481 EFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDL 540
           EFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VN V+AK+ H PWLS  +INHY L
Sbjct: 481 EFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYRL 540

Query: 541 HKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 557
             FYW+MC LS  NFLH+LFWA+KYKYRST  +K
Sbjct: 541 SNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of CmoCh05G009630 vs. ExPASy TrEMBL
Match: A0A1P8SN46 (NRT1.2-like nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.2C PE=2 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 4.3e-242
Identity = 443/575 (77.04%), Postives = 502/575 (87.30%), Query Frame = 0

Query: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60
           ME VKEE DG +WEGYVDWR+RPA +GRHGGM AAGFVLGVEVLENLAFLANASNLVM+L
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAAKGRHGGMIAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120
           RKYM FSP +SANHVT FMGTAFLLALLGGFLSDA FTTYYVF+FSS +EFLGLV+LTV+
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAIFTTYYVFIFSSFIEFLGLVMLTVQ 120

Query: 121 AKAATAA--------------SAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGR 180
           AK +++                 AV+F+GLYLVA+GVGGIKGSLP HGAEQF+E T +GR
Sbjct: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180

Query: 181 RGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYR 240
           + RSSFFNYFVFSLSCGA++AVT VVW+EDNLGW+WGFGISTI+I +SIPLF AGS FYR
Sbjct: 181 KRRSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240

Query: 241 NKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTT-TPKP--TKEPT-HTT 300
           NKIP G+PLTTILKVLVAATLN RS  K A+NAVAS+ RSPSTT TPK    K+ T +  
Sbjct: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLTENNV 300

Query: 301 PPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFS 360
             PTQ+LKFLNKA+Q PPFHPSL CTTQQLEDVK+V+K+LPIFACTIILNSCLAQLSTFS
Sbjct: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360

Query: 361 VEQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRI 420
           +EQA+TMNTKI S+K+PPASLP+FP+LFII+LAPLY+HLIIPFAR+LTRTE+GITHLQRI
Sbjct: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420

Query: 421 GVGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGL 480
           GVGLLLS++AMA+AAL+E KRKA+ADA+P  H PLPITFLWI FQYLFLGSADLFTLAGL
Sbjct: 421 GVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480

Query: 481 LEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYD 540
           LEFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VN V+AK+ H PWLS  +INHY 
Sbjct: 481 LEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYR 540

Query: 541 LHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 557
           L  FYW+MC LS LNFLH+LFWA+KYKYRST  +K
Sbjct: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575

BLAST of CmoCh05G009630 vs. NCBI nr
Match: XP_022946066.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata])

HSP 1 Score: 1057.4 bits (2733), Expect = 4.4e-305
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60
           MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL
Sbjct: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60

Query: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120
           RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE
Sbjct: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120

Query: 121 AKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSL 180
           AKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSL
Sbjct: 121 AKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSL 180

Query: 181 SCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILK 240
           SCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILK
Sbjct: 181 SCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILK 240

Query: 241 VLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP 300
           VLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP
Sbjct: 241 VLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP 300

Query: 301 FHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP 360
           FHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP
Sbjct: 301 FHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP 360

Query: 361 ASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE 420
           ASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE
Sbjct: 361 ASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE 420

Query: 421 VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL 480
           VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL
Sbjct: 421 VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL 480

Query: 481 SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF 540
           SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF
Sbjct: 481 SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF 540

Query: 541 LFWAIKYKYRSTLASK 557
           LFWAIKYKYRSTLASK
Sbjct: 541 LFWAIKYKYRSTLASK 556

BLAST of CmoCh05G009630 vs. NCBI nr
Match: XP_023546035.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.8e-298
Identity = 543/556 (97.66%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60
           MEVVKEEGD GVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL
Sbjct: 1   MEVVKEEGDPGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60

Query: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120
           RKYMQFSPT+SANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE
Sbjct: 61  RKYMQFSPTKSANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120

Query: 121 AKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSL 180
           AKAATAASAAVVFVGLYLVAVGVGGIKGSLP HGAEQ EEGTAEGR+GRSSFFNYFVFSL
Sbjct: 121 AKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSFFNYFVFSL 180

Query: 181 SCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILK 240
           SCGA+VAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPA APLTTILK
Sbjct: 181 SCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAAAPLTTILK 240

Query: 241 VLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP 300
           VLVAA LNGR RNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP
Sbjct: 241 VLVAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPP 300

Query: 301 FHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP 360
           FHPSLSCTTQQLEDVK+VLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP
Sbjct: 301 FHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPP 360

Query: 361 ASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE 420
           ASLPIFP+LFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE
Sbjct: 361 ASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIE 420

Query: 421 VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL 480
           VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL
Sbjct: 421 VKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL 480

Query: 481 SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF 540
           SWASLAVGYYLSSVIVSLVN VTAKSSHSPWLS QDINHYDLHKFYWLMCALSALNFLHF
Sbjct: 481 SWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHF 540

Query: 541 LFWAIKYKYRSTLASK 557
           LFWAIKYKYRSTLASK
Sbjct: 541 LFWAIKYKYRSTLASK 556

BLAST of CmoCh05G009630 vs. NCBI nr
Match: XP_022999670.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima])

HSP 1 Score: 1000.0 bits (2584), Expect = 8.3e-288
Identity = 524/557 (94.08%), Postives = 539/557 (96.77%), Query Frame = 0

Query: 1   MEVVKEEGD-GGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMF 60
           MEVVKEEGD GGVWEGYVDWRRRPAVRGRHGGMRAA FVLGVEVLENLAFLANASNLVMF
Sbjct: 1   MEVVKEEGDGGGVWEGYVDWRRRPAVRGRHGGMRAAVFVLGVEVLENLAFLANASNLVMF 60

Query: 61  LRKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTV 120
           LRKYMQFSP +SANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTV
Sbjct: 61  LRKYMQFSPAKSANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTV 120

Query: 121 EAKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFS 180
           EAKAA+AASAA VFVGLYLVAVGVGGIKGSL  HGAEQFEEGTAEGRRGRSSFFNYFVFS
Sbjct: 121 EAKAASAASAAAVFVGLYLVAVGVGGIKGSLAAHGAEQFEEGTAEGRRGRSSFFNYFVFS 180

Query: 181 LSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTIL 240
           LSCGA+V VTLVVW+EDNLGWEWGFGISTIAILVSIPLFL+GSCFYRNKIP  APLTTIL
Sbjct: 181 LSCGALVGVTLVVWIEDNLGWEWGFGISTIAILVSIPLFLSGSCFYRNKIPTAAPLTTIL 240

Query: 241 KVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTP 300
           KVLVAATLNGR RNKTA+NAVASMTRSPSTTTPKPTKEPTHTT PPTQTLKFLNKAIQTP
Sbjct: 241 KVLVAATLNGRRRNKTATNAVASMTRSPSTTTPKPTKEPTHTT-PPTQTLKFLNKAIQTP 300

Query: 301 PFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLP 360
           PFHPSLSCTTQQLEDVK+VLKLLPIFACTIILNSCLAQLSTFSVEQASTMNT+IGSIK P
Sbjct: 301 PFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTQIGSIKFP 360

Query: 361 PASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALI 420
           PASLPIFP++FII+LAPLYNHLI+PFARTLTRTESGITHLQRIGVGLLLSIVAM IAALI
Sbjct: 361 PASLPIFPVIFIIILAPLYNHLIVPFARTLTRTESGITHLQRIGVGLLLSIVAMVIAALI 420

Query: 421 EVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATA 480
           EVKRKA+ADAHPAAHPLPITFLWI FQYLFLGSADLFTLAGLLEFFFTEAP TMRSLATA
Sbjct: 421 EVKRKAVADAHPAAHPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPPTMRSLATA 480

Query: 481 LSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLH 540
           LSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLS QDINHYDLHKFYWLMCALSALNFLH
Sbjct: 481 LSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLH 540

Query: 541 FLFWAIKYKYRSTLASK 557
           FLFWA+KYKYRST+  K
Sbjct: 541 FLFWALKYKYRSTIERK 556

BLAST of CmoCh05G009630 vs. NCBI nr
Match: XP_038886228.1 (protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida])

HSP 1 Score: 879.4 bits (2271), Expect = 1.6e-251
Identity = 459/573 (80.10%), Postives = 511/573 (89.18%), Query Frame = 0

Query: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60
           ME+VKEE DG +WEGYVDWR+R AVRGRHGGM AAGFVLGVEVLENLAFLANASNLVM+L
Sbjct: 1   MEIVKEESDGSIWEGYVDWRKRAAVRGRHGGMMAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120
           RKYM+FSP +SANHVT FMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLV+LTV+
Sbjct: 61  RKYMEFSPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVMLTVQ 120

Query: 121 AKAATAA--------------SAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGR 180
           AK +++                AAV+F+GLYLVA+GVGGIKGSLP HGAEQF+EG+ +GR
Sbjct: 121 AKVSSSCEEGNSKGCKEVGGREAAVLFIGLYLVALGVGGIKGSLPAHGAEQFDEGSPQGR 180

Query: 181 RGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYR 240
           + RSSFFNYFVFSLSCGA++AVT VVW+EDNLGW+WGFGISTI+I +SIPLFLAGS FYR
Sbjct: 181 KRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAGSPFYR 240

Query: 241 NKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKP--TKEPT-HTTP 300
           NKIP G+PLTTILKVLVAA LN  S NK A+NAVAS+ RSPST+T K    KE T + T 
Sbjct: 241 NKIPTGSPLTTILKVLVAAALNRGSSNKPATNAVASLARSPSTSTAKQLINKELTQNNTT 300

Query: 301 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSV 360
            PTQ+LKFLNKA+Q PPFHPSL CTTQQLEDVK+VLK+LPIFACTIILNSCLAQLSTFS+
Sbjct: 301 NPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQLSTFSI 360

Query: 361 EQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 420
           EQASTMNTKI S+K+PPASLPIFP+LFIILLAPLY+HLIIPFARTLTRTE+GITHLQRIG
Sbjct: 361 EQASTMNTKISSLKVPPASLPIFPVLFIILLAPLYDHLIIPFARTLTRTEAGITHLQRIG 420

Query: 421 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 480
           VGLLLS++AMA+AAL+E KRKA+A AHP AHPLPITFLWI FQYLFLGSADLFTLAGLLE
Sbjct: 421 VGLLLSVLAMAVAALVETKRKAVAGAHPDAHPLPITFLWIAFQYLFLGSADLFTLAGLLE 480

Query: 481 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLH 540
           FFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VNRVTAK+SH+PWLS  +INHY L 
Sbjct: 481 FFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNINHYRLS 540

Query: 541 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 557
            FYWLMCALS  NFLH+LFWA+KYKYRST  +K
Sbjct: 541 NFYWLMCALSGFNFLHYLFWALKYKYRSTQGNK 573

BLAST of CmoCh05G009630 vs. NCBI nr
Match: XP_008456458.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa] >TYK00047.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 854.0 bits (2205), Expect = 7.3e-244
Identity = 447/574 (77.87%), Postives = 504/574 (87.80%), Query Frame = 0

Query: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60
           ME VKEE DG +WEGYVDWR+RPAV+GRHGGM AAGFVLGVEVLENLAFLANASNLVM+L
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120
           RKYM FSP +SANHVT FMGTAFLLALLGGFLSDAFFTTYYVFLFSS LEFLGLV+LTV+
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQ 120

Query: 121 AKAATAASA-------------AVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRR 180
           AK +++  A             AV+F+GLYLVA+GVGGIKGSLP HGAEQF+E T +GR+
Sbjct: 121 AKVSSSCEAGTKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGRK 180

Query: 181 GRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRN 240
            RSSFFNYFVFSLSCGA+VAVT VVW+EDNLGW+WGFGISTI+  +SIPLF AGS FYRN
Sbjct: 181 RRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYRN 240

Query: 241 KIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTT-TPKP--TKEPTHT-TP 300
           KIP G+PLTTILKVLVAATLN RS  K A+NAVAS+ RSPSTT TPKP   K+ T + T 
Sbjct: 241 KIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDLTESNTT 300

Query: 301 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSV 360
            PTQ+LKFLNKA+Q PPFHPS+ CTTQQLEDVK+V+K+LPIFACTIILNSCLAQLSTFS+
Sbjct: 301 TPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFSI 360

Query: 361 EQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 420
           EQA+TMNTKI S+K+PPASLP+FP+LFII+LAPLY+HLIIPFAR+LTRTE+GITHLQRIG
Sbjct: 361 EQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGITHLQRIG 420

Query: 421 VGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGLL 480
           VGLLLS++AMA+AAL+E KRKA+ADA+P    PLPITFLWI FQYLFLGSADLFTLAGLL
Sbjct: 421 VGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLFTLAGLL 480

Query: 481 EFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDL 540
           EFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VN V+AK+ H PWLS  +INHY L
Sbjct: 481 EFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYRL 540

Query: 541 HKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 557
             FYW+MC LS  NFLH+LFWA+KYKYRST  +K
Sbjct: 541 SNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of CmoCh05G009630 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 701.8 bits (1810), Expect = 4.3e-202
Identity = 377/575 (65.57%), Postives = 458/575 (79.65%), Query Frame = 0

Query: 13  WEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKYMQFSPTESA 72
           WEGY DWR R AV+GRHGGM AA FVL VE+LENLA+LANASNLV++LR+YM  SP++SA
Sbjct: 10  WEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSA 69

Query: 73  NHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEAKA--------- 132
           N VTNFMGTAFLLALLGGFLSDAFF+T+ +FL S+ +EFLGL+ILT++A+          
Sbjct: 70  NDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCD 129

Query: 133 ------ATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFV 192
                  + + AA++FVGLYLVA+GVGGIKGSL +HGAEQF+E T +GR+ RS+FFNYFV
Sbjct: 130 SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFV 189

Query: 193 FSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTT 252
           F L+CGA+VAVT VVW+EDN GWEWGFG+STIAI VSI +FL+GS FYRNKIP G+PLTT
Sbjct: 190 FCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTT 249

Query: 253 ILKVLVAATLNGRSRNKTASNAVASMTRSPST--------------TTPKPTKEPTHTTP 312
           ILKVL+AA++   S + ++SNAVASM+ SPS                  KP +E     P
Sbjct: 250 ILKVLLAASVKCCS-SGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQE--EALP 309

Query: 313 PP---TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLST 372
           P    T +LK LN A    P H  L CT QQ+EDVK+VLK+LPIFACTI+LN CLAQLST
Sbjct: 310 PRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLST 369

Query: 373 FSVEQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQ 432
           FSV+QA++MNTKIGS+K+PPASLPIFP++FI++LAP+Y+HLIIPFAR  T+TE+G+THLQ
Sbjct: 370 FSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQ 429

Query: 433 RIGVGLLLSIVAMAIAALIEVKRKAIADAH---PAAHPLPITFLWIGFQYLFLGSADLFT 492
           RIGVGL+LSI+AMA+AAL+E+KRK +A       +   LP+TFLWI  QYLFLGSADLFT
Sbjct: 430 RIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFT 489

Query: 493 LAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDI 552
           LAGLLE+FFTEAPS+MRSLAT+LSWASLA+GYYLSSVIVS+VN +T  S ++PWL  + I
Sbjct: 490 LAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSI 549

BLAST of CmoCh05G009630 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 645.6 bits (1664), Expect = 3.7e-185
Identity = 350/570 (61.40%), Postives = 436/570 (76.49%), Query Frame = 0

Query: 4   VKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKY 63
           V+  GD   WEGY DWR + A+RGRHGGM AA FVL VE+LENLAFLANASNLV++L+ +
Sbjct: 3   VEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNF 62

Query: 64  MQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEAKA 123
           M  S   S++ VT FM TAFLLALLGGFL+DAFF+T+ +FL S+ +EFLGL++LT++A+ 
Sbjct: 63  MHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARR 122

Query: 124 AT------------------AASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEG 183
            +                   + AA +FVGLYLV++G+GGIKGSLP+HGAEQF+EGT +G
Sbjct: 123 PSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKG 182

Query: 184 RRGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFY 243
           R+ RS+FFNY+VF LSCGA+VAVT VVW+EDN GWEWGFG+STI+I +SI +FL GS FY
Sbjct: 183 RKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFY 242

Query: 244 RNKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPP 303
           +NKIP G+PLTTI KVL+AA++   S +KT+SN   S        +    K P+ +    
Sbjct: 243 KNKIPRGSPLTTIFKVLLAASIVSCS-SKTSSNHFTSR----EVQSEHEEKTPSQSL--- 302

Query: 304 TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQ 363
           T +L  LNKAI+    H  L CT QQ+EDVK+VLK+LPIF CTI+LN CLAQLST+SV Q
Sbjct: 303 TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQ 362

Query: 364 ASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVG 423
           A+TMN KI +  +P ASLP+FP++F+++LAP Y+HLIIPFAR +T++E GITHLQRIGVG
Sbjct: 363 AATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVG 422

Query: 424 LLLSIVAMAIAALIEVKRKAI---ADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLL 483
           L+LSIVAMA+AAL+E+KRK +   A    +   LPITFLWI  QYLFLGSADLFTLAGLL
Sbjct: 423 LVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLL 482

Query: 484 EFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDL 543
           EFFFTEAPS+MRSLAT+LSWASLA+GYYLSSV+V +VNRVT  +  SPWL  + +N   L
Sbjct: 483 EFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRNRL 542

Query: 544 HKFYWLMCALSALNFLHFLFWAIKYKYRST 553
             FYWLMC LS +NFLH+LFWA +YKY ST
Sbjct: 543 DLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of CmoCh05G009630 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 644.0 bits (1660), Expect = 1.1e-184
Identity = 349/566 (61.66%), Postives = 434/566 (76.68%), Query Frame = 0

Query: 8   GDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKYMQFS 67
           GD   WEGY DWR + A+RGRHGGM AA FVL VE+LENLAFLANASNLV++L+ +M  S
Sbjct: 3   GDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMS 62

Query: 68  PTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEAKAAT-- 127
              S++ VT FM TAFLLALLGGFL+DAFF+T+ +FL S+ +EFLGL++LT++A+  +  
Sbjct: 63  LARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLM 122

Query: 128 ----------------AASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGR 187
                            + AA +FVGLYLV++G+GGIKGSLP+HGAEQF+EGT +GR+ R
Sbjct: 123 PPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQR 182

Query: 188 SSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKI 247
           S+FFNY+VF LSCGA+VAVT VVW+EDN GWEWGFG+STI+I +SI +FL GS FY+NKI
Sbjct: 183 STFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKI 242

Query: 248 PAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTL 307
           P G+PLTTI KVL+AA++   S +KT+SN   S        +    K P+ +    T +L
Sbjct: 243 PRGSPLTTIFKVLLAASIVSCS-SKTSSNHFTSR----EVQSEHEEKTPSQSL---TNSL 302

Query: 308 KFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTM 367
             LNKAI+    H  L CT QQ+EDVK+VLK+LPIF CTI+LN CLAQLST+SV QA+TM
Sbjct: 303 TCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATM 362

Query: 368 NTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLS 427
           N KI +  +P ASLP+FP++F+++LAP Y+HLIIPFAR +T++E GITHLQRIGVGL+LS
Sbjct: 363 NRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLS 422

Query: 428 IVAMAIAALIEVKRKAI---ADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFF 487
           IVAMA+AAL+E+KRK +   A    +   LPITFLWI  QYLFLGSADLFTLAGLLEFFF
Sbjct: 423 IVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFF 482

Query: 488 TEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFY 547
           TEAPS+MRSLAT+LSWASLA+GYYLSSV+V +VNRVT  +  SPWL  + +N   L  FY
Sbjct: 483 TEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRNRLDLFY 542

Query: 548 WLMCALSALNFLHFLFWAIKYKYRST 553
           WLMC LS +NFLH+LFWA +YKY ST
Sbjct: 543 WLMCVLSVVNFLHYLFWAKRYKYIST 559

BLAST of CmoCh05G009630 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 634.4 bits (1635), Expect = 8.5e-182
Identity = 340/561 (60.61%), Postives = 430/561 (76.65%), Query Frame = 0

Query: 2   EVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLR 61
           E + E      W GYVDWR RPA+RGRHGGM AA FVL VEVLENLAFLANASNLV++L 
Sbjct: 30  EQMDEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLS 89

Query: 62  KYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEA 121
             M FSP+ +AN VT FMGTAF LALLGGFL+DAFFTT++++L S+ +EFLGL++LTV+A
Sbjct: 90  TKMGFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQA 149

Query: 122 KAATAA--SAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFS 181
              +    S   +FVGLYLVA+GVGGIKGSLP HGAEQF+E T+ GRR RS FFNYF+FS
Sbjct: 150 HEHSTEPWSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFS 209

Query: 182 LSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTIL 241
           LSCGA++AVT+VVW+EDN GW +GFG+ST AIL+S+P+FLAGS  YR K+P+G+P+TT+ 
Sbjct: 210 LSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSPITTLF 269

Query: 242 KVLVAATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTP 301
           KVL AA      + +T+   V   TR+    +   TK+  +          FL + ++  
Sbjct: 270 KVLTAALYAKYKKRRTSRIVVTCHTRNDCDDS--VTKQ--NCDGDDGFLGSFLGEVVRER 329

Query: 302 PFHP-SLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKL 361
              P  L CT +Q++DVK+V+K+LPIF  TI+LN CLAQLSTFSV+QASTMNTK+GS  +
Sbjct: 330 ESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFSVQQASTMNTKLGSFTV 389

Query: 362 PPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAAL 421
           PPA+LP+FP++F+++LAP YNHL++P AR  T+TE+GITHLQRIG GL+LSIVAMA+AAL
Sbjct: 390 PPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAAL 449

Query: 422 IEVKRKAIA--------DAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAP 481
           +E KRK +          +  ++ PLPITFLW+  QY+FLGSADLFTLAG++EFFFTEAP
Sbjct: 450 VETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEAP 509

Query: 482 STMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMC 541
           STMRSLAT+LSWASLA+GYY SSV+VS VN VT  + H+PWL  +++N Y L +FYWLMC
Sbjct: 510 STMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGENLNQYHLERFYWLMC 569

Query: 542 ALSALNFLHFLFWAIKYKYRS 552
            LS +NFLH+LFWA +Y YRS
Sbjct: 570 VLSGINFLHYLFWASRYVYRS 586

BLAST of CmoCh05G009630 vs. TAIR 10
Match: AT3G25260.1 (Major facilitator superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.9e-96
Identity = 224/547 (40.95%), Postives = 338/547 (61.79%), Query Frame = 0

Query: 14  EGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFLRKYMQFSPTESAN 73
           E + DWR + A+ G+HGG++AA     VE +EN+ FLA ++N +M+  K M +S  ++A 
Sbjct: 7   EKFEDWRGKEAISGKHGGIKAAFIACVVETMENMVFLACSTNFMMYFTKSMNYSTPKAAT 66

Query: 74  HVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVEA----------KA 133
            VTNF+GT+FLL + GGF++D+F T +  F+    +E LGL++LT++A          K 
Sbjct: 67  MVTNFVGTSFLLTIFGGFVADSFLTRFAAFVLFGSIELLGLIMLTLQAHITKLQPQGGKK 126

Query: 134 ATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSFFNYFVFSLSCG 193
            +   + V+F GLY +A+GVGG+KGSLP HG +Q   GT   +R  S FFN++ FS+  G
Sbjct: 127 PSTPQSTVLFTGLYAIAIGVGGVKGSLPAHGGDQI--GT-RNQRLISGFFNWYFFSVCLG 186

Query: 194 AVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAGAPLTTILKVLV 253
             +AVTL+VW+E+N+GW   F IST  +  +I +F+AG   YR K PAG+PLT I+ V V
Sbjct: 187 GFLAVTLMVWIEENIGWSSSFTISTAVLASAIFVFVAGCPMYRFKRPAGSPLTRIVNVFV 246

Query: 254 AATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPPFHP 313
           +A    R+RN+  ++A   +T++ ++T     K   H         KFLNKA      + 
Sbjct: 247 SA---ARNRNRFVTDAEV-VTQNHNST----DKSIHH------NKFKFLNKA----KLNN 306

Query: 314 SLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIG-SIKLPPAS 373
            +S T  Q+E+ +  L LLPIF  TII+N C+AQ+ TFSV+Q    N K+  S ++P AS
Sbjct: 307 KISAT--QVEETRTFLALLPIFGSTIIMNCCVAQMGTFSVQQGMVTNRKLSRSFEIPVAS 366

Query: 374 LPIFPILFIILLAPLYNHLIIPFARTLTRTE-SGITHLQRIGVGLLLSIVAMAIAALIEV 433
           L   P+L ++    LY    +   R L+ +E S   +L+RIG GL L+ ++MA+AA++EV
Sbjct: 367 LNAIPLLCMLSSLALYE---LFGKRILSNSERSSSFNLKRIGYGLALTSISMAVAAIVEV 426

Query: 434 KRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATAL 493
           KRK     H A H  + I+  W+  Q++ L  +D+ T+ G+LEFFF E+P++MRS++TAL
Sbjct: 427 KRK-----HEAVHNNIKISVFWLELQFVMLSLSDMLTVGGMLEFFFRESPASMRSMSTAL 486

Query: 494 SWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLHKFYWLMCALSALNFLHF 548
            W S A+G++LSSV+V +VN +T       WL   D+N   L  FY ++C L+ LN  ++
Sbjct: 487 GWCSTAMGFFLSSVLVEVVNGITG------WL-RDDLNESRLELFYLVLCVLNTLNLFNY 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1576.1e-20165.57Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE45.2e-18461.40Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9FM201.2e-18060.61Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Q9LSF02.6e-9540.95Protein NRT1/ PTR FAMILY 4.1 OS=Arabidopsis thaliana OX=3702 GN=NPF4.1 PE=2 SV=1[more]
Q9LSE81.0e-9438.98Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G2M62.1e-305100.00protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111450266 ... [more]
A0A6J1KG664.0e-28894.08protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111493955 PE... [more]
A0A5D3BLL03.5e-24477.87Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C3A13.5e-24477.87protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 S... [more]
A0A1P8SN464.3e-24277.04NRT1.2-like nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.2C PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022946066.14.4e-305100.00protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata][more]
XP_023546035.11.8e-29897.66protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo][more]
XP_022999670.18.3e-28894.08protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima][more]
XP_038886228.11.6e-25180.10protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida][more]
XP_008456458.17.3e-24477.87PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protei... [more]
Match NameE-valueIdentityDescription
AT1G69850.14.3e-20265.57nitrate transporter 1:2 [more]
AT1G27040.13.7e-18561.40Major facilitator superfamily protein [more]
AT1G27040.21.1e-18461.66Major facilitator superfamily protein [more]
AT5G62730.18.5e-18260.61Major facilitator superfamily protein [more]
AT3G25260.11.9e-9640.95Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 4..554
e-value: 2.5E-156
score: 522.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 34..550
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 102..511
e-value: 6.3E-97
score: 324.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 15..553
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 254..287
NoneNo IPR availablePANTHERPTHR11654:SF490PROTEIN NRT1/ PTR FAMILY 4.6-LIKEcoord: 15..553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G009630.1CmoCh05G009630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity