CmoCh05G009450 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh05G009450
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Terpene cyclase/mutase family member
Location	Cmo_Chr05: 7560661 .. 7561224 (-)
RNA-Seq Expression	CmoCh05G009450
Synteny	CmoCh05G009450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCATGGAATGGGTAAAATTAGATATGCAGAATGCACGTCATCATCGATAGAGGCACTTATTCTCTTTAAGAAGCTATTTCCAAATTACAGAAAGAAAGAGATAGAATTTTTTTTAAGTAAAGCAGTAAAGTATTTGGAAGAAACGCAGAAAGAGGATGGATCATGGTATGGAAATTGGGGAATTTGTCACATTTACGCAACATACTTTGCTATAAAAGGATTAGTGGCAACTGGAAACACTTACCAAAATTCCTCAACCCTAAGAAGAGGTGTGGAGTTTCTTCTTAAAATTGAATGTCCAGACGGTGGGTGGGGAGAGAGTCACATTTCATGTACACAAACGAAATACATTCCTCTTCTACGGAATGCTTCCAATCTTGTTCAAACTTCCTTTGCTTTAATAGCTTTGATCCATTCTCAGCAGGTTTGTTTTACTCTTTTACTTCAATTATTATAAGTACGTAAAGCTTTTTTTATATATATAATAAAGAAATGGCGTAAGTATGTAGGAAAAGAGAGACCCAACTCCTCTTCACCGTGCGGCAAAACTATTGATTAA

mRNA sequence

ATGAGCCATGGAATGGGTAAAATTAGATATGCAGAATGCACGTCATCATCGATAGAGGCACTTATTCTCTTTAAGAAGCTATTTCCAAATTACAGAAAGAAAGAGATAGAATTTTTTTTAAGTAAAGCAGTAAAGTATTTGGAAGAAACGCAGAAAGAGGATGGATCATGGTATGGAAATTGGGGAATTTGTCACATTTACGCAACATACTTTGCTATAAAAGGATTAGTGGCAACTGGAAACACTTACCAAAATTCCTCAACCCTAAGAAGAGGTGTGGAGTTTCTTCTTAAAATTGAATGTCCAGACGGTGGGTGGGGAGAGAGTCACATTTCATGTACACAAACGAAATACATTCCTCTTCTACGGAATGCTTCCAATCTTGTTCAAACTTCCTTTGCTTTAATAGCTTTGATCCATTCTCAGCAGAAATGGCGTAAGTATGTAGGAAAAGAGAGACCCAACTCCTCTTCACCGTGCGGCAAAACTATTGATTAA

Coding sequence (CDS)

ATGAGCCATGGAATGGGTAAAATTAGATATGCAGAATGCACGTCATCATCGATAGAGGCACTTATTCTCTTTAAGAAGCTATTTCCAAATTACAGAAAGAAAGAGATAGAATTTTTTTTAAGTAAAGCAGTAAAGTATTTGGAAGAAACGCAGAAAGAGGATGGATCATGGTATGGAAATTGGGGAATTTGTCACATTTACGCAACATACTTTGCTATAAAAGGATTAGTGGCAACTGGAAACACTTACCAAAATTCCTCAACCCTAAGAAGAGGTGTGGAGTTTCTTCTTAAAATTGAATGTCCAGACGGTGGGTGGGGAGAGAGTCACATTTCATGTACACAAACGAAATACATTCCTCTTCTACGGAATGCTTCCAATCTTGTTCAAACTTCCTTTGCTTTAATAGCTTTGATCCATTCTCAGCAGAAATGGCGTAAGTATGTAGGAAAAGAGAGACCCAACTCCTCTTCACCGTGCGGCAAAACTATTGATTAA

Protein sequence

MSHGMGKIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLVQTSFALIALIHSQQKWRKYVGKERPNSSSPCGKTID

Homology

[Close]

BLAST of CmoCh05G009450 vs. ExPASy Swiss-Prot
Match: O82140 (Beta-amyrin synthase 1 OS=Panax ginseng OX=4054 GN=OSCPNY1 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 7.4e-46
Identity = 81/137 (59.12%), Postives = 106/137 (77.37%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSS+I+AL+LFKKL+P +RKKEI+ F++ AV+YLE+TQ  DGSWYGNWG+C  Y +
Sbjct: 562 YVECTSSAIQALVLFKKLYPGHRKKEIDNFITNAVRYLEDTQMPDGSWYGNWGVCFTYGS 621

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           +FA+ GL A G TY N + +R+ VEFLLK +  DGGWGES++SC +  Y+PL  N SNLV
Sbjct: 622 WFALGGLAAAGKTYYNCAAVRKAVEFLLKSQMDDGGWGESYLSCPKKVYVPLEGNRSNLV 681

Query: 130 QTSFALIALIHSQQKWR 147
            T +AL+ LIHS+Q  R
Sbjct: 682 HTGWALMGLIHSEQAER 698

[Close]

BLAST of CmoCh05G009450 vs. ExPASy Swiss-Prot
Match: Q9LRH7 (Mixed-amyrin synthase OS=Pisum sativum OX=3888 GN=OSCPSM PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 3.7e-45
Identity = 80/137 (58.39%), Postives = 107/137 (78.10%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSS+I+AL+LFKKL+P +RKKE+E F++ AV++LE  Q  DGSWYGNWGIC  Y +
Sbjct: 561 YVECTSSAIQALVLFKKLYPEHRKKEVENFIANAVRFLEYKQTSDGSWYGNWGICFTYGS 620

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           +FA+ GLVA G TY N + +R+GVEFLL  +  DGGWGESH+S ++  Y+PL R+ SN+V
Sbjct: 621 WFALNGLVAAGKTYDNCAAIRKGVEFLLTTQREDGGWGESHLSSSKKIYVPLERSQSNIV 680

Query: 130 QTSFALIALIHSQQKWR 147
           QTS+A++ LIH+ Q  R
Sbjct: 681 QTSWAIMGLIHAGQMER 697

[Close]

BLAST of CmoCh05G009450 vs. ExPASy Swiss-Prot
Match: E2IUA8 (Friedelin synthase OS=Kalanchoe daigremontiana OX=23013 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.1e-44
Identity = 77/134 (57.46%), Postives = 103/134 (76.87%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSS+++AL+LFKKL+P +R+KE+E F++   KYLE+ Q  DGSWYGNWG+C  Y  
Sbjct: 561 YVECTSSAVQALVLFKKLYPLHRRKEVERFITNGAKYLEDIQMPDGSWYGNWGVCFTYGA 620

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           +FA++GL A G TY N + +R+GV+FLL I+  DGGWGES+ SC   KY+PL  N SNLV
Sbjct: 621 WFALEGLSAAGKTYNNCAAVRKGVDFLLNIQLEDGGWGESYQSCPDKKYVPLEDNRSNLV 680

Query: 130 QTSFALIALIHSQQ 144
           QTS+AL+ LI++ Q
Sbjct: 681 QTSWALMGLIYAGQ 694

[Close]

BLAST of CmoCh05G009450 vs. ExPASy Swiss-Prot
Match: E2IUA9 (Lupeol synthase OS=Kalanchoe daigremontiana OX=23013 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.1e-44
Identity = 77/134 (57.46%), Postives = 103/134 (76.87%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSS+++AL+LFKKL+P +R+KE+E F++   KYLE+ Q  DGSWYGNWG+C  Y  
Sbjct: 561 YVECTSSAVQALVLFKKLYPLHRRKEVERFITNGAKYLEDIQMPDGSWYGNWGVCFTYGA 620

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           +FA++GL A G TY N + +R+GV+FLL I+  DGGWGES+ SC   KY+PL  N SNLV
Sbjct: 621 WFALEGLSAAGKTYNNCAAVRKGVDFLLNIQLEDGGWGESYQSCPDKKYVPLEDNRSNLV 680

Query: 130 QTSFALIALIHSQQ 144
           QTS+AL+ LI++ Q
Sbjct: 681 QTSWALMGLIYAGQ 694

[Close]

BLAST of CmoCh05G009450 vs. ExPASy Swiss-Prot
Match: O82146 (Beta-amyrin synthase 2 OS=Panax ginseng OX=4054 GN=OSCPNY2 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 5.3e-44
Identity = 78/137 (56.93%), Postives = 103/137 (75.18%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSS+I+A+++FKKL+P +RKKEIE  ++ AV+YLE+ Q  DGSWYGNWG+C  Y T
Sbjct: 560 YVECTSSAIQAMVMFKKLYPGHRKKEIEVSITNAVQYLEDIQMPDGSWYGNWGVCFTYGT 619

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           +FA+ GL A G TY N  TL + V+FL+K +  DGGWGES++SC   +Y PL  N SNLV
Sbjct: 620 WFAMGGLTAAGKTYNNCQTLHKAVDFLIKSQRSDGGWGESYLSCPNKEYTPLEGNRSNLV 679

Query: 130 QTSFALIALIHSQQKWR 147
            TS+A++ LIHS+Q  R
Sbjct: 680 HTSWAMMGLIHSRQAER 696

[Close]

BLAST of CmoCh05G009450 vs. ExPASy TrEMBL
Match: A0A6J1G4P4 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450753 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.3e-64
Identity = 117/137 (85.40%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSSSI+AL+LF KLFPN+RKKEIE FLSKA+KYLEETQKEDGSW+GNWG+CHIYAT
Sbjct: 496 YVECTSSSIQALVLFTKLFPNHRKKEIETFLSKAIKYLEETQKEDGSWFGNWGVCHIYAT 555

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           YFAIKGLVATGNTY NSST+R+GVEFLLKI+CPDGGWGESH+SC Q KYIPL RN+SNLV
Sbjct: 556 YFAIKGLVATGNTYHNSSTIRKGVEFLLKIQCPDGGWGESHVSCMQKKYIPLPRNSSNLV 615

Query: 130 QTSFALIALIHSQQKWR 147
           QTSFAL+ALIHSQQK R
Sbjct: 616 QTSFALMALIHSQQKKR 632

[Close]

BLAST of CmoCh05G009450 vs. ExPASy TrEMBL
Match: A0A6J1G4L5 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450753 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.3e-64
Identity = 117/137 (85.40%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSSSI+AL+LF KLFPN+RKKEIE FLSKA+KYLEETQKEDGSW+GNWG+CHIYAT
Sbjct: 525 YVECTSSSIQALVLFTKLFPNHRKKEIETFLSKAIKYLEETQKEDGSWFGNWGVCHIYAT 584

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           YFAIKGLVATGNTY NSST+R+GVEFLLKI+CPDGGWGESH+SC Q KYIPL RN+SNLV
Sbjct: 585 YFAIKGLVATGNTYHNSSTIRKGVEFLLKIQCPDGGWGESHVSCMQKKYIPLPRNSSNLV 644

Query: 130 QTSFALIALIHSQQKWR 147
           QTSFAL+ALIHSQQK R
Sbjct: 645 QTSFALMALIHSQQKKR 661

[Close]

BLAST of CmoCh05G009450 vs. ExPASy TrEMBL
Match: A0A6J1G4T2 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450753 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.3e-64
Identity = 117/137 (85.40%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSSSI+AL+LF KLFPN+RKKEIE FLSKA+KYLEETQKEDGSW+GNWG+CHIYAT
Sbjct: 565 YVECTSSSIQALVLFTKLFPNHRKKEIETFLSKAIKYLEETQKEDGSWFGNWGVCHIYAT 624

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           YFAIKGLVATGNTY NSST+R+GVEFLLKI+CPDGGWGESH+SC Q KYIPL RN+SNLV
Sbjct: 625 YFAIKGLVATGNTYHNSSTIRKGVEFLLKIQCPDGGWGESHVSCMQKKYIPLPRNSSNLV 684

Query: 130 QTSFALIALIHSQQKWR 147
           QTSFAL+ALIHSQQK R
Sbjct: 685 QTSFALMALIHSQQKKR 701

[Close]

BLAST of CmoCh05G009450 vs. ExPASy TrEMBL
Match: A0A6J1G3U8 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 6.3e-64
Identity = 116/138 (84.06%), Postives = 129/138 (93.48%), Query Frame = 0


Query: 7   KIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHI 66
           ++ Y ECTSSS++AL+LF KLFPN+RKKEIE FLSKAV Y+EETQKEDGSWYGNWGICHI
Sbjct: 562 ELEYVECTSSSVQALVLFTKLFPNHRKKEIETFLSKAVIYMEETQKEDGSWYGNWGICHI 621

Query: 67  YATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNAS 126
           YATYFAIKGLVATGNTY NSST+R+GV+FLLKI+CPDGGWGESHISCTQ KYIPL  N+S
Sbjct: 622 YATYFAIKGLVATGNTYHNSSTIRKGVDFLLKIQCPDGGWGESHISCTQKKYIPLPENSS 681

Query: 127 NLVQTSFALIALIHSQQK 145
           NLVQTSFAL+ALIHSQQK
Sbjct: 682 NLVQTSFALMALIHSQQK 699

[Close]

BLAST of CmoCh05G009450 vs. ExPASy TrEMBL
Match: A0A6J1G3X4 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 8.2e-64
Identity = 116/140 (82.86%), Postives = 130/140 (92.86%), Query Frame = 0


Query: 7   KIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHI 66
           ++ Y ECTSSS++AL+LF KLFPN+RKKEIE FLSKAV Y+EETQKEDGSWYGNWGICHI
Sbjct: 562 ELEYVECTSSSVQALVLFTKLFPNHRKKEIETFLSKAVIYMEETQKEDGSWYGNWGICHI 621

Query: 67  YATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNAS 126
           YATYFAIKGLVATGNTY NSST+R+GV+FLLKI+CPDGGWGESHISCTQ KYIPL  N+S
Sbjct: 622 YATYFAIKGLVATGNTYHNSSTIRKGVDFLLKIQCPDGGWGESHISCTQKKYIPLPENSS 681

Query: 127 NLVQTSFALIALIHSQQKWR 147
           NLVQTSFAL+ALIHSQQ+ R
Sbjct: 682 NLVQTSFALMALIHSQQEKR 701

[Close]

BLAST of CmoCh05G009450 vs. NCBI nr
Match: XP_023520470.1 (LOW QUALITY PROTEIN: beta-Amyrin Synthase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256.5 bits (654), Expect = 1.5e-64
Identity = 118/140 (84.29%), Postives = 131/140 (93.57%), Query Frame = 0


Query: 7   KIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHI 66
           ++ Y ECTSSS++AL+LF KLFPN+RKKEIE FLSKAVKY+EETQKEDGSWYGNWGICHI
Sbjct: 497 ELEYVECTSSSVQALVLFTKLFPNHRKKEIETFLSKAVKYMEETQKEDGSWYGNWGICHI 556

Query: 67  YATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNAS 126
           YATYFAIKGLVATGNTY NSST+R+GVEFLLKI+CPDGGWGESHISCTQ KYIPL  N+S
Sbjct: 557 YATYFAIKGLVATGNTYHNSSTIRKGVEFLLKIQCPDGGWGESHISCTQKKYIPLPGNSS 616

Query: 127 NLVQTSFALIALIHSQQKWR 147
           NLVQTSFAL+ALIHSQQ+ R
Sbjct: 617 NLVQTSFALMALIHSQQEKR 636

[Close]

BLAST of CmoCh05G009450 vs. NCBI nr
Match: XP_022946792.1 (lupeol synthase-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 255.8 bits (652), Expect = 2.6e-64
Identity = 117/137 (85.40%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSSSI+AL+LF KLFPN+RKKEIE FLSKA+KYLEETQKEDGSW+GNWG+CHIYAT
Sbjct: 496 YVECTSSSIQALVLFTKLFPNHRKKEIETFLSKAIKYLEETQKEDGSWFGNWGVCHIYAT 555

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           YFAIKGLVATGNTY NSST+R+GVEFLLKI+CPDGGWGESH+SC Q KYIPL RN+SNLV
Sbjct: 556 YFAIKGLVATGNTYHNSSTIRKGVEFLLKIQCPDGGWGESHVSCMQKKYIPLPRNSSNLV 615

Query: 130 QTSFALIALIHSQQKWR 147
           QTSFAL+ALIHSQQK R
Sbjct: 616 QTSFALMALIHSQQKKR 632

[Close]

BLAST of CmoCh05G009450 vs. NCBI nr
Match: XP_022946788.1 (beta-amyrin synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 255.8 bits (652), Expect = 2.6e-64
Identity = 117/137 (85.40%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSSSI+AL+LF KLFPN+RKKEIE FLSKA+KYLEETQKEDGSW+GNWG+CHIYAT
Sbjct: 565 YVECTSSSIQALVLFTKLFPNHRKKEIETFLSKAIKYLEETQKEDGSWFGNWGVCHIYAT 624

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           YFAIKGLVATGNTY NSST+R+GVEFLLKI+CPDGGWGESH+SC Q KYIPL RN+SNLV
Sbjct: 625 YFAIKGLVATGNTYHNSSTIRKGVEFLLKIQCPDGGWGESHVSCMQKKYIPLPRNSSNLV 684

Query: 130 QTSFALIALIHSQQKWR 147
           QTSFAL+ALIHSQQK R
Sbjct: 685 QTSFALMALIHSQQKKR 701

[Close]

BLAST of CmoCh05G009450 vs. NCBI nr
Match: XP_022946791.1 (beta-Amyrin Synthase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 255.8 bits (652), Expect = 2.6e-64
Identity = 117/137 (85.40%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 10  YAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYAT 69
           Y ECTSSSI+AL+LF KLFPN+RKKEIE FLSKA+KYLEETQKEDGSW+GNWG+CHIYAT
Sbjct: 525 YVECTSSSIQALVLFTKLFPNHRKKEIETFLSKAIKYLEETQKEDGSWFGNWGVCHIYAT 584

Query: 70  YFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNLV 129
           YFAIKGLVATGNTY NSST+R+GVEFLLKI+CPDGGWGESH+SC Q KYIPL RN+SNLV
Sbjct: 585 YFAIKGLVATGNTYHNSSTIRKGVEFLLKIQCPDGGWGESHVSCMQKKYIPLPRNSSNLV 644

Query: 130 QTSFALIALIHSQQKWR 147
           QTSFAL+ALIHSQQK R
Sbjct: 645 QTSFALMALIHSQQKKR 661

[Close]

BLAST of CmoCh05G009450 vs. NCBI nr
Match: XP_022946464.1 (beta-amyrin synthase-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 253.4 bits (646), Expect = 1.3e-63
Identity = 116/138 (84.06%), Postives = 129/138 (93.48%), Query Frame = 0


Query: 7   KIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHI 66
           ++ Y ECTSSS++AL+LF KLFPN+RKKEIE FLSKAV Y+EETQKEDGSWYGNWGICHI
Sbjct: 562 ELEYVECTSSSVQALVLFTKLFPNHRKKEIETFLSKAVIYMEETQKEDGSWYGNWGICHI 621

Query: 67  YATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNAS 126
           YATYFAIKGLVATGNTY NSST+R+GV+FLLKI+CPDGGWGESHISCTQ KYIPL  N+S
Sbjct: 622 YATYFAIKGLVATGNTYHNSSTIRKGVDFLLKIQCPDGGWGESHISCTQKKYIPLPENSS 681

Query: 127 NLVQTSFALIALIHSQQK 145
           NLVQTSFAL+ALIHSQQK
Sbjct: 682 NLVQTSFALMALIHSQQK 699

[Close]

BLAST of CmoCh05G009450 vs. TAIR 10
Match: AT1G78960.1 (lupeol synthase 2 )

HSP 1 Score: 174.9 bits (442), Expect = 5.5e-44
Identity = 78/142 (54.93%), Postives = 107/142 (75.35%), Query Frame = 0


Query: 5   MGKIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGIC 64
           M +  Y ECTS+ I+AL+LFK+L+P++R KEI   + K V+++E  Q  DGSW+GNWGIC
Sbjct: 557 MAEREYVECTSAVIQALVLFKQLYPDHRTKEIIKSIEKGVQFIESKQTPDGSWHGNWGIC 616

Query: 65  HIYATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRN 124
            IYAT+FA+ GL A G TY++   +R+GV+FLL I+  DGGWGESH+SC + +YIPL  N
Sbjct: 617 FIYATWFALSGLAAAGKTYKSCLAVRKGVDFLLAIQEEDGGWGESHLSCPEQRYIPLEGN 676

Query: 125 ASNLVQTSFALIALIHSQQKWR 147
            SNLVQT++A++ LIH+ Q  R
Sbjct: 677 RSNLVQTAWAMMGLIHAGQAER 698

[Close]

BLAST of CmoCh05G009450 vs. TAIR 10
Match: AT1G78970.2 (lupeol synthase 1 )

HSP 1 Score: 173.7 bits (439), Expect = 1.2e-43
Identity = 79/153 (51.63%), Postives = 110/153 (71.90%), Query Frame = 0


Query: 1   MSHGMGKIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGN 60
           M++ M +  + ECTSS I+AL LF+KL+P++RKKEI   + KAV+++++ Q  DGSWYGN
Sbjct: 550 MANTMVEREFVECTSSVIQALDLFRKLYPDHRKKEINRSIEKAVQFIQDNQTPDGSWYGN 609

Query: 61  WGICHIYATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIP 120
           WG+C IYAT+FA+ GL A G TY +   +R GV FLL  +  DGGWGES++SC++ +YIP
Sbjct: 610 WGVCFIYATWFALGGLAAAGETYNDCLAMRNGVHFLLTTQRDDGGWGESYLSCSEQRYIP 669

Query: 121 LLRNASNLVQTSFALIALIHSQQKWRKYVGKER 154
                SNLVQTS+A++ALIH+ Q  R  +   R
Sbjct: 670 SEGERSNLVQTSWAMMALIHTGQAERDLIPLHR 702

[Close]

BLAST of CmoCh05G009450 vs. TAIR 10
Match: AT1G78970.1 (lupeol synthase 1 )

HSP 1 Score: 173.7 bits (439), Expect = 1.2e-43
Identity = 79/153 (51.63%), Postives = 110/153 (71.90%), Query Frame = 0


Query: 1   MSHGMGKIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGN 60
           M++ M +  + ECTSS I+AL LF+KL+P++RKKEI   + KAV+++++ Q  DGSWYGN
Sbjct: 550 MANTMVEREFVECTSSVIQALDLFRKLYPDHRKKEINRSIEKAVQFIQDNQTPDGSWYGN 609

Query: 61  WGICHIYATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIP 120
           WG+C IYAT+FA+ GL A G TY +   +R GV FLL  +  DGGWGES++SC++ +YIP
Sbjct: 610 WGVCFIYATWFALGGLAAAGETYNDCLAMRNGVHFLLTTQRDDGGWGESYLSCSEQRYIP 669

Query: 121 LLRNASNLVQTSFALIALIHSQQKWRKYVGKER 154
                SNLVQTS+A++ALIH+ Q  R  +   R
Sbjct: 670 SEGERSNLVQTSWAMMALIHTGQAERDLIPLHR 702

[Close]

BLAST of CmoCh05G009450 vs. TAIR 10
Match: AT5G48010.1 (thalianol synthase 1 )

HSP 1 Score: 168.3 bits (425), Expect = 5.1e-42
Identity = 74/138 (53.62%), Postives = 96/138 (69.57%), Query Frame = 0


Query: 9   RYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNWGICHIYA 68
           RY ECT S+I AL  F K FP Y+  E++ F++KA KY+E+ Q  DGSWYGNWG+C IY 
Sbjct: 558 RYVECTGSAIAALTQFNKQFPGYKNVEVKRFITKAAKYIEDMQTVDGSWYGNWGVCFIYG 617

Query: 69  TYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPLLRNASNL 128
           T+FA++GLVA G TY N   +R+ V FLL  + P+GGWGES +SC   KY PL  N++N+
Sbjct: 618 TFFAVRGLVAAGKTYSNCEAIRKAVRFLLDTQNPEGGWGESFLSCPSKKYTPLKGNSTNV 677

Query: 129 VQTSFALIALIHSQQKWR 147
           VQT+ AL+ LI   Q  R
Sbjct: 678 VQTAQALMVLIMGDQMER 695

[Close]

BLAST of CmoCh05G009450 vs. TAIR 10
Match: AT1G66960.1 (Terpenoid cyclases family protein )

HSP 1 Score: 167.9 bits (424), Expect = 6.7e-42
Identity = 74/148 (50.00%), Postives = 107/148 (72.30%), Query Frame = 0


Query: 2   SHGMGKIRYAECTSSSIEALILFKKLFPNYRKKEIEFFLSKAVKYLEETQKEDGSWYGNW 61
           ++ M +  Y ECTS+ ++AL++F +L+P++R KEI   + KAV+++E  Q  DGSWYG+W
Sbjct: 554 ANAMTEREYTECTSAVLQALVIFNQLYPDHRTKEITKSIEKAVQFIESKQLRDGSWYGSW 613

Query: 62  GICHIYATYFAIKGLVATGNTYQNSSTLRRGVEFLLKIECPDGGWGESHISCTQTKYIPL 121
           GIC  Y T+FA+ GL A G TY N  ++R GV FLL I+  DGGWGES++SC + +YIPL
Sbjct: 614 GICFTYGTWFALCGLAAIGKTYNNCLSMRDGVHFLLNIQNEDGGWGESYMSCPEQRYIPL 673

Query: 122 LRNASNLVQTSFALIALIHSQQKWRKYV 150
             N SN+VQT++A++ALIH+ Q  R  +
Sbjct: 674 EGNRSNVVQTAWAMMALIHAGQAKRDLI 701

The following BLAST results are available for this feature:

BLAST of CmoCh05G009450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82140	7.4e-46	59.12	Beta-amyrin synthase 1 OS=Panax ginseng OX=4054 GN=OSCPNY1 PE=1 SV=1	[more]
Q9LRH7	3.7e-45	58.39	Mixed-amyrin synthase OS=Pisum sativum OX=3888 GN=OSCPSM PE=1 SV=1	[more]
E2IUA8	4.1e-44	57.46	Friedelin synthase OS=Kalanchoe daigremontiana OX=23013 PE=1 SV=1	[more]
E2IUA9	4.1e-44	57.46	Lupeol synthase OS=Kalanchoe daigremontiana OX=23013 PE=1 SV=1	[more]
O82146	5.3e-44	56.93	Beta-amyrin synthase 2 OS=Panax ginseng OX=4054 GN=OSCPNY2 PE=2 SV=1	[more]

BLAST of CmoCh05G009450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4P4	1.3e-64	85.40	Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507...	[more]
A0A6J1G4L5	1.3e-64	85.40	Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507...	[more]
A0A6J1G4T2	1.3e-64	85.40	Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507...	[more]
A0A6J1G3U8	6.3e-64	84.06	Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505...	[more]
A0A6J1G3X4	8.2e-64	82.86	Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505...	[more]

BLAST of CmoCh05G009450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520470.1	1.5e-64	84.29	LOW QUALITY PROTEIN: beta-Amyrin Synthase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022946792.1	2.6e-64	85.40	lupeol synthase-like isoform X3 [Cucurbita moschata]	[more]
XP_022946788.1	2.6e-64	85.40	beta-amyrin synthase-like isoform X1 [Cucurbita moschata]	[more]
XP_022946791.1	2.6e-64	85.40	beta-Amyrin Synthase 1-like isoform X2 [Cucurbita moschata]	[more]
XP_022946464.1	1.3e-63	84.06	beta-amyrin synthase-like isoform X4 [Cucurbita moschata]	[more]

BLAST of CmoCh05G009450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78960.1	5.5e-44	54.93	lupeol synthase 2	[more]
AT1G78970.2	1.2e-43	51.63	lupeol synthase 1	[more]
AT1G78970.1	1.2e-43	51.63	lupeol synthase 1	[more]
AT5G48010.1	5.1e-42	53.62	thalianol synthase 1	[more]
AT1G66960.1	6.7e-42	50.00	Terpenoid cyclases family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032696	Squalene cyclase, C-terminal	PFAM	PF13243	SQHop_cyclase_C	coord: 29..144 e-value: 5.9E-24 score: 84.7
None	No IPR available	GENE3D	1.50.10.20		coord: 2..158 e-value: 1.1E-45 score: 157.9
None	No IPR available	PANTHER	PTHR11764:SF52	AMYRIN SYNTHASE LUP2-RELATED	coord: 5..147
IPR018333	Squalene cyclase	PANTHER	PTHR11764	TERPENE CYCLASE/MUTASE FAMILY MEMBER	coord: 5..147
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 4..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh05G009450.1	CmoCh05G009450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016104	triterpenoid biosynthetic process
cellular_component	GO:0005811	lipid droplet
molecular_function	GO:0042300	beta-amyrin synthase activity
molecular_function	GO:0000250	lanosterol synthase activity
molecular_function	GO:0016866	intramolecular transferase activity