CmoCh05G009430 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G009430
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionTerpene cyclase/mutase family member
LocationCmo_Chr05: 7506445 .. 7510097 (-)
RNA-Seq ExpressionCmoCh05G009430
SyntenyCmoCh05G009430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAGGCTTAAGTTGGGAGAAGGAGCAAATGATCCTTACTTATTCAGCTCCAATAACTTTATGGGAAGACAAACGTGGGATTTCGAGCCCGATGAAGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCCCGCCAAAATTATTACCATAATCGTTTCAAAGTTCCATGTAGCAGTGACCTTTTTTGGAAATTTCAGGTGGCTTTAAATCATTTCAAATATTTATTTATTTTAAGTTTCTAACTTTAATGATCCTATTGTACTTTCCTAAATTTAAATCTAATTTATTTAGAAATGGAATTATAATAAGTTATTGAATATTATGAGAATGCAGTTTCTAAGAGAGCGAAATTTCAAGCAAACAATTCCAATAGTGAAAGTGGAGGAAGCAATAATTAATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAAGTTCTTTGCAGCCTTACAAAGCCACCATGGCCATTGGCCTGCTGAAAACTCTGGCCCTATGTTTTACTTCCCTCCACTGGTACTCTTCCTTTTTCTATTCATTTTATCTAATATAATTCTATCACAATATTACCCTTTTCTTTTCAGGTATTTTCGTTGTATATTACGGGACATCTCGGCATTATATTCTCAGAAGAGCACCGAAAGGAAATCCTTCGCTATGCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGGGAAGAACCTGACAAGGATGAGTGTTTTAGAGCTCGAAAATGGATTCTAGATCATGGAGGTGCTATTTATATACCGTCCTGGGGAAAGATTTGGCTATCGGTTAGTACTGTATATATACGTCCATACTATTTTATTTGAAACAGTGTAACTCAAATTTAACGAACTAAACTTATAATTTAACTTTGTAGATTTTGGGAGTTTACGAGTGGGAGGGAGCAAACCCTATGCCTCCAGAGTTTTGGCTATTAGGAAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCGAGATTGACACTTCTTCCTATGTCGTATTTATTTGGGAAGCGATTCGTTGGACCTCTCACCCCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAATCCAACACGCCATTATTGTGCAAAGGTACATTTCTAACCCATTCAATCCTCCCTACAACAATGCACGAGTAAAGAAAATCAATATCGATCCTTTCAAAAGGTTTCAGATTTGTAGAGGTAGAAATTGATCATTTCTATTTCATTTACCGTATGAGACTTTGATTACTTTGATTGAATTGGACATGATGATGTAGGAAGACAAATGCTTTGAACGTTCTTTATTTCAGAAGCTTGCATGGGATGCTCTTCAATACTTTGGAGAACCCATTCTTAATAGTTGGGCTTTTAAAACAATAAGAAATAGAGCCCTTCAAATAGCTAAACGTCATATTGATTATGAAGATCATAACAGTCATTACATTACAATCGGATGCATTGAAAAGGTTAATTTCATACTTCTTTTTTCTTTTAGGTCATAATTATAGATAATAATATTATTATTAATAAATATAATTGAGCTAATATGGTGGAGGGGTATTGAACATGCAGCCATTGTTCACACTTGCTTGTTGGGTCGATGATCCTAATGGGGAAGCTTACAAGAAACATGTTGCTAGAATCAAAGATTACTTATGGATTGCTGAAGATGGAATGAAGATGCAAGTAAGTATTACCCATTATTAAATACTTCGCTAATTATAAACAAATAAATATAATCTTTGCCTTAAAATATCAATTACAATCCAACCTTTAAATGGATGATTTATGAAACTTTGTTATAAATATACATAGCAAATAATAAGAGAAATATATAAAGGACTAACATATATGTATACGAGCAGAGTTTTGGTAGTCAATCATGGGACGTTGCTTTTGCCATTCAAGCAATTCTTGCAACAAATCTCCACCATGAATTCTCGGAGACACTTAAGAAAGGGCATGACTTCATTAAACAATCACAGGTACTAAAATTCTTTAATTTCAATAAATATTTGTTTATATTATTTTAACGTATATTCATATAAAGGTTAGAGAGAATCCTTCGAGTGATTTTCGAAGTATGTATCGTCACATATCAAAAGGAAGTTGGACATTCTCCGATCAAGATCATGGATGGCAAGTTTGTGATGATACTGCAGAAAATTTATTGGTATTTTATTTTATTTTCATACTTTCCATAAAAAGATATTAAAATTTAAATCAATTTACTAATCTCACATATATATATAAATCAAATGATGTGTCAATGTTGTTTGATATTTTTGACCATGCCTTCCAACATAGTAGGAGATCCAATGGAACCACAATGGTTTTTTGAAGCCGTCAATATCATACTATCCTTTCAAGTAAGGATTGTAATTTCGAATTTGATTTATAATTATCTTTTTCTTGTCAACTTAATGATAATTGGTGAATTTTGTTGTGAAATAAATAGGCAAAAAATGGTGGAGTCTCAGCTTGGGAGCCTACCGGAGGTGTACCATCGTGGTTTGAGGTACCTAAGGCCTTATTTGATAATTATTTTCTCAAACAATCATTAATAAAATTAAAGAGTGGAGACAGCTCATTAATATCATTAATTTGTTTTATCTTATACTTTTTTTAGCGACTGAATCCAGTAGAGTTCTTCGAATACACCATATTAGAGCTTGAGTAAGTAGACAATCTAAGAGTACTATATGCACGTAAATTGCATTGGACTGATTATTATTTGTTTTGTTTTCCTTGTGATAAATTATGGGATGGGTAAAATTAGATACGTAGAATGCACGTTAGCGTCAATCCAGGCACTTATTCTATTTAAGAAGCTATTTCCAAGTCATAGAAAGAAAGAGATAGAAACGTTTTTAAGTAAAGCAGTAAAGTATTTGGAAGAAACGCAGATAGAGGATGGATCATGGTATGGAAATTGGGGAATTTGTCACATTTATGCAACATACTTCGCTATAAAAGGATTAGTGGCATCTGGAAACACTTACCAAAATTCCTCAACCCTACAAAGAGGTGTGGAGTTTCTTCTTAAAATTCAATGTCCAGACGGTGGGTGGGGAGAGAGTCACATTTCATGTAGAGAAAAGAAATACATTCCTCTTCCGGGGAATTCTTCCAATCTTGTTCAAACTTCCTTTGCTTTAATAGCTTTGATCCTTTCTCAACAGGTATGTTTTACTCTTTTACTTCAATTATTATAAGTACGTAAAGCTTTTATATATATAATAAAGAAATGGCGTATGTATGTAGGAAAAGAGAGACCCAACTCCTCTTCACCGTGGAGCAAAGCTATTGATTAATTCTCAACTGGAGAATGGTGATTACCCTCAACAGGTAGGTTACTGTAATAGGTGTGTTTTGGTTAAACAATAACTTTTGTTAAATTAATAATTCATTATATTAAAAAAGAAAATTATTTATATGGCAGGAGATAAGTGGAGTATTTATGAACACATGCATGCTACACTATGGACTATACAAGAACGTATTCCCATTGTGGGCACTTGCAGAGTATTGCAATAAGGTTTCATTGCCATAG

mRNA sequence

ATGTGGAGGCTTAAGTTGGGAGAAGGAGCAAATGATCCTTACTTATTCAGCTCCAATAACTTTATGGGAAGACAAACGTGGGATTTCGAGCCCGATGAAGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCCCGCCAAAATTATTACCATAATCGTTTCAAAGTTCCATGTAGCAGTGACCTTTTTTGGAAATTTCAGAAATGGAATTATAATAAGTTATTGAATATTATGAGAATGCAGTTTCTAAGAGAGCGAAATTTCAAGCAAACAATTCCAATAGTGAAAGTGGAGGAAGCAATAATTAATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAAGTTCTTTGCAGCCTTACAAAGCCACCATGGCCATTGGCCTGCTGAAAACTCTGGCCCTATGTTTTACTTCCCTCCACTGGTATTTTCGTTGTATATTACGGGACATCTCGGCATTATATTCTCAGAAGAGCACCGAAAGGAAATCCTTCGCTATGCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGGGAAGAACCTGACAAGGATGAGTGTTTTAGAGCTCGAAAATGGATTCTAGATCATGGAGGTGCTATTTATATACCGTCCTGGGGAAAGATTTGGCTATCGGTTAGTACTATTTTGGGAGTTTACGAGTGGGAGGGAGCAAACCCTATGCCTCCAGAGTTTTGGCTATTAGGAAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCGAGATTGACACTTCTTCCTATGTCGTATTTATTTGGGAAGCGATTCGTTGGACCTCTCACCCCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAATCCAACACGCCATTATTGTGCAAAGGAAGACAAATGCTTTGAACGTTCTTTATTTCAGAAGCTTGCATGGGATGCTCTTCAATACTTTGGAGAACCCATTCTTAATAGTTGGGCTTTTAAAACAATAAGAAATAGAGCCCTTCAAATAGCTAAACGTCATATTGATTATGAAGATCATAACAGTCATTACATTACAATCGGATGCATTGAAAAGGTTAATTTCATACTTCTTTTTTCTTTTAGTCAATCATGGGACGTTGCTTTTGCCATTCAAGCAATTCTTGCAACAAATCTCCACCATGAATTCTCGGAGACACTTAAGAAAGGGCATGACTTCATTAAACAATCACAGGTTAGAGAGAATCCTTCGAGTGATTTTCGAAGTATGTATCGTCACATATCAAAAGGAAGTTGGACATTCTCCGATCAAGATCATGGATGGCAAGTTTGTGATGATACTGCAGAAAATTTATTGGCAAAAAATGGTGGAGTCTCAGCTTGGGAGCCTACCGGAGGTGTACCATCGTGGTTTGAGCGACTGAATCCAGTAGAGTTCTTCGAATACACCATATTAGAGCTTGAATACGTAGAATGCACGTTAGCGTCAATCCAGGCACTTATTCTATTTAAGAAGCTATTTCCAAGTCATAGAAAGAAAGAGATAGAAACGTTTTTAAGTAAAGCAGTAAAGTATTTGGAAGAAACGCAGATAGAGGATGGATCATGGTATGGAAATTGGGGAATTTGTCACATTTATGCAACATACTTCGCTATAAAAGGATTAGTGGCATCTGGAAACACTTACCAAAATTCCTCAACCCTACAAAGAGGTGTGGAGTTTCTTCTTAAAATTCAATGTCCAGACGGTGGGTGGGGAGAGAGTCACATTTCATGTAGAGAAAAGAAATACATTCCTCTTCCGGGGAATTCTTCCAATCTTGTTCAAACTTCCTTTGCTTTAATAGCTTTGATCCTTTCTCAACAGGAAAAGAGAGACCCAACTCCTCTTCACCGTGGAGCAAAGCTATTGATTAATTCTCAACTGGAGAATGGTGATTACCCTCAACAGGTAGGTTACTGTAATAGGTGTGTTTTGGAGATAAGTGGAGTATTTATGAACACATGCATGCTACACTATGGACTATACAAGAACGTATTCCCATTGTGGGCACTTGCAGAGTATTGCAATAAGGTTTCATTGCCATAG

Coding sequence (CDS)

ATGTGGAGGCTTAAGTTGGGAGAAGGAGCAAATGATCCTTACTTATTCAGCTCCAATAACTTTATGGGAAGACAAACGTGGGATTTCGAGCCCGATGAAGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCCCGCCAAAATTATTACCATAATCGTTTCAAAGTTCCATGTAGCAGTGACCTTTTTTGGAAATTTCAGAAATGGAATTATAATAAGTTATTGAATATTATGAGAATGCAGTTTCTAAGAGAGCGAAATTTCAAGCAAACAATTCCAATAGTGAAAGTGGAGGAAGCAATAATTAATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAAGTTCTTTGCAGCCTTACAAAGCCACCATGGCCATTGGCCTGCTGAAAACTCTGGCCCTATGTTTTACTTCCCTCCACTGGTATTTTCGTTGTATATTACGGGACATCTCGGCATTATATTCTCAGAAGAGCACCGAAAGGAAATCCTTCGCTATGCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGGGAAGAACCTGACAAGGATGAGTGTTTTAGAGCTCGAAAATGGATTCTAGATCATGGAGGTGCTATTTATATACCGTCCTGGGGAAAGATTTGGCTATCGGTTAGTACTATTTTGGGAGTTTACGAGTGGGAGGGAGCAAACCCTATGCCTCCAGAGTTTTGGCTATTAGGAAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCGAGATTGACACTTCTTCCTATGTCGTATTTATTTGGGAAGCGATTCGTTGGACCTCTCACCCCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAATCCAACACGCCATTATTGTGCAAAGGAAGACAAATGCTTTGAACGTTCTTTATTTCAGAAGCTTGCATGGGATGCTCTTCAATACTTTGGAGAACCCATTCTTAATAGTTGGGCTTTTAAAACAATAAGAAATAGAGCCCTTCAAATAGCTAAACGTCATATTGATTATGAAGATCATAACAGTCATTACATTACAATCGGATGCATTGAAAAGGTTAATTTCATACTTCTTTTTTCTTTTAGTCAATCATGGGACGTTGCTTTTGCCATTCAAGCAATTCTTGCAACAAATCTCCACCATGAATTCTCGGAGACACTTAAGAAAGGGCATGACTTCATTAAACAATCACAGGTTAGAGAGAATCCTTCGAGTGATTTTCGAAGTATGTATCGTCACATATCAAAAGGAAGTTGGACATTCTCCGATCAAGATCATGGATGGCAAGTTTGTGATGATACTGCAGAAAATTTATTGGCAAAAAATGGTGGAGTCTCAGCTTGGGAGCCTACCGGAGGTGTACCATCGTGGTTTGAGCGACTGAATCCAGTAGAGTTCTTCGAATACACCATATTAGAGCTTGAATACGTAGAATGCACGTTAGCGTCAATCCAGGCACTTATTCTATTTAAGAAGCTATTTCCAAGTCATAGAAAGAAAGAGATAGAAACGTTTTTAAGTAAAGCAGTAAAGTATTTGGAAGAAACGCAGATAGAGGATGGATCATGGTATGGAAATTGGGGAATTTGTCACATTTATGCAACATACTTCGCTATAAAAGGATTAGTGGCATCTGGAAACACTTACCAAAATTCCTCAACCCTACAAAGAGGTGTGGAGTTTCTTCTTAAAATTCAATGTCCAGACGGTGGGTGGGGAGAGAGTCACATTTCATGTAGAGAAAAGAAATACATTCCTCTTCCGGGGAATTCTTCCAATCTTGTTCAAACTTCCTTTGCTTTAATAGCTTTGATCCTTTCTCAACAGGAAAAGAGAGACCCAACTCCTCTTCACCGTGGAGCAAAGCTATTGATTAATTCTCAACTGGAGAATGGTGATTACCCTCAACAGGTAGGTTACTGTAATAGGTGTGTTTTGGAGATAAGTGGAGTATTTATGAACACATGCATGCTACACTATGGACTATACAAGAACGTATTCCCATTGTGGGCACTTGCAGAGTATTGCAATAAGGTTTCATTGCCATAG

Protein sequence

MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSSDLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEAIINKETASIALRRATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFILLFSFSQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSMYRHISKGSWTFSDQDHGWQVCDDTAENLLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSHRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQRGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPLHRGAKLLINSQLENGDYPQQVGYCNRCVLEISGVFMNTCMLHYGLYKNVFPLWALAEYCNKVSLP
Homology
BLAST of CmoCh05G009430 vs. ExPASy Swiss-Prot
Match: O82140 (Beta-amyrin synthase 1 OS=Panax ginseng OX=4054 GN=OSCPNY1 PE=1 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 2.2e-259
Identity = 438/786 (55.73%), Postives = 533/786 (67.81%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFMGRQTWDFEPD-EGTPEERAEVEEARQNYYHNRFKVPC 60
           MW+LK+ EG  NDPYL+S+NNF+GRQTW+F+PD   +P E  EVE+ R+ ++ NR++V  
Sbjct: 1   MWKLKIAEGNKNDPYLYSTNNFVGRQTWEFDPDYVASPGELEEVEQVRRQFWDNRYQVKP 60

Query: 61  SSDLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKV-EEAIINKETASIALRRATKF 120
           S DL W              RMQFLRE+NF+QTIP VKV ++  +  E A+  LRRA  F
Sbjct: 61  SGDLLW--------------RMQFLREKNFRQTIPQVKVGDDEAVTYEAATTTLRRAVHF 120

Query: 121 FAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGG 180
           F+ALQ+  GHWPAENSGP+F+ PPLV  +YITGHL  +F  EHRKEILRY YCHQNEDGG
Sbjct: 121 FSALQASDGHWPAENSGPLFFLPPLVMCVYITGHLDTVFPAEHRKEILRYIYCHQNEDGG 180

Query: 181 WGLHIEGQSCMLCTVFNYIQLRLLGEEPD---KDECFRARKWILDHGGAIYIPSWGKIWL 240
           WGLHIEG S M CT  +YI +R+LGE PD    + C R RKWILDHG    IPSWGK WL
Sbjct: 181 WGLHIEGHSTMFCTTLSYICMRILGEGPDGGVNNACARGRKWILDHGSVTAIPSWGKTWL 240

Query: 241 SVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTP 300
           S   ILGVYEW G+NPMPPEFW+L   LP  P  ++CY R+  +PMSYL+GKRFVGP+TP
Sbjct: 241 S---ILGVYEWIGSNPMPPEFWILPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITP 300

Query: 301 LILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFK 360
           LILQLR+E+Y QPYN I W  TR  CAKED  +   L Q L WD+L    EP+L  W F 
Sbjct: 301 LILQLREELYGQPYNEINWRKTRRVCAKEDIYYPHPLIQDLLWDSLYVLTEPLLTRWPFN 360

Query: 361 TIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------ 420
            +R +ALQ   +HI YED NS YITIGC+EKV  +L                        
Sbjct: 361 KLREKALQTTMKHIHYEDENSRYITIGCVEKVLCMLVCWVEDPNGDYFRKHLARIPDYIW 420

Query: 421 -------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRS 480
                  + SF SQ WD  F+IQA+L ++L HE   TL KGHDFIK+SQV++NPS DF+S
Sbjct: 421 VAEDGMKMQSFGSQEWDTGFSIQALLDSDLTHEIGPTLMKGHDFIKKSQVKDNPSGDFKS 480

Query: 481 MYRHISKGSWTFSDQDHGWQVCDDTAE--------------------------------- 540
           MYRHISKGSWTFSDQDHGWQV D TAE                                 
Sbjct: 481 MYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLIFSTMPEEIVGKKIKPERLYDSVNVLL 540

Query: 541 NLLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPS 600
           +L  KNGG+SAWEP  G   W E LNP EFF   ++E EYVECT ++IQAL+LFKKL+P 
Sbjct: 541 SLQRKNGGLSAWEP-AGAQEWLELLNPTEFFADIVIEHEYVECTSSAIQALVLFKKLYPG 600

Query: 601 HRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQ 660
           HRKKEI+ F++ AV+YLE+TQ+ DGSWYGNWG+C  Y ++FA+ GL A+G TY N + ++
Sbjct: 601 HRKKEIDNFITNAVRYLEDTQMPDGSWYGNWGVCFTYGSWFALGGLAAAGKTYYNCAAVR 660

Query: 661 RGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTP 716
           + VEFLLK Q  DGGWGES++SC +K Y+PL GN SNLV T +AL+ LI S+Q +RDPTP
Sbjct: 661 KAVEFLLKSQMDDGGWGESYLSCPKKVYVPLEGNRSNLVHTGWALMGLIHSEQAERDPTP 720

BLAST of CmoCh05G009430 vs. ExPASy Swiss-Prot
Match: Q8W3Z1 (Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 1.8e-258
Identity = 429/785 (54.65%), Postives = 537/785 (68.41%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLK+ +G +DPY++S+NNF+GRQTW+F+P  G+P+ERAEVEEAR+N+Y NR++V  S 
Sbjct: 1   MWRLKIADGGSDPYIYSTNNFVGRQTWEFDPQAGSPQERAEVEEARRNFYDNRYQVKPSG 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEA-IINKETASIALRRATKFFA 120
           DL W              RMQFL+E+NFKQTIP VKVE+   I  E ++ ALRRA  F++
Sbjct: 61  DLLW--------------RMQFLKEKNFKQTIPPVKVEDGEEITYEKSTAALRRAVHFYS 120

Query: 121 ALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWG 180
           ALQ+  GHWPAEN+GP+F+ PPLV  +YITGHL  +F  EH+KEILRY Y HQNEDGGWG
Sbjct: 121 ALQASDGHWPAENAGPLFFLPPLVMCMYITGHLNTVFPAEHQKEILRYIYYHQNEDGGWG 180

Query: 181 LHIEGQSCMLCTVFNYIQLRLLGEEPD---KDECFRARKWILDHGGAIYIPSWGKIWLSV 240
           LHIEG S M CT  +YI +R+LGE PD    + C RARKWILDHGG  ++PSWGK WLS 
Sbjct: 181 LHIEGHSTMFCTALSYICMRILGEGPDGGQDNACARARKWILDHGGVTHMPSWGKTWLS- 240

Query: 241 STILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLI 300
             ILG++EW G+NPMPPEFW+L   LP  P  ++CY R+  +PMSYL+GKRFVGP+TPLI
Sbjct: 241 --ILGIFEWIGSNPMPPEFWILPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLI 300

Query: 301 LQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAF-KT 360
           LQLR+E+YTQPY+ + W   RH CAKED  +   L Q L WD+L  F EP+L  W F K 
Sbjct: 301 LQLREELYTQPYHQVNWKKVRHLCAKEDIYYPHPLIQDLLWDSLYIFTEPLLTRWPFNKL 360

Query: 361 IRNRALQIAKRHIDYEDHNSHYITIGCIEKV----------------------------- 420
           +R +ALQ+  +HI YED NS YITIGC+EKV                             
Sbjct: 361 VREKALQVTMKHIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKKHIARIPDYIWV 420

Query: 421 --NFILLFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSM 480
             + I + SF SQ WD  FAIQA+LA+NL  E   TL +GHDFIK+SQV++NPS DF SM
Sbjct: 421 AEDGIKMQSFGSQEWDTGFAIQALLASNLTDEIGPTLARGHDFIKKSQVKDNPSGDFESM 480

Query: 481 YRHISKGSWTFSDQDHGWQVCDDTAE---------------------------------N 540
           +RHISKGSWTFSDQDHGWQV D TAE                                 +
Sbjct: 481 HRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSIMPPEIVGEKMEPEQLYDSVNVLLS 540

Query: 541 LLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSH 600
           L +KNGG++AWEP  G   W E LN  EFF   ++E EY+ECT +++Q L+LFKKL+P H
Sbjct: 541 LQSKNGGLAAWEP-AGAQEWLELLNSTEFFADIVIEHEYIECTASAMQTLVLFKKLYPGH 600

Query: 601 RKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQR 660
           RKKEIE F+  A ++L+  Q+ DGSWYGNWG+C  Y T+FA+ GL A G TY N   ++R
Sbjct: 601 RKKEIENFIKNAAQFLQVIQMPDGSWYGNWGVCFTYGTWFALGGLAAVGKTYNNCLAVRR 660

Query: 661 GVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPL 716
            V+FLL+ Q  +GGWGES++SC +K+Y+PL GN SNLV T++A++ LI + Q +RDPTPL
Sbjct: 661 AVDFLLRAQRDNGGWGESYLSCPKKEYVPLEGNKSNLVHTAWAMMGLIHAGQAERDPTPL 720

BLAST of CmoCh05G009430 vs. ExPASy Swiss-Prot
Match: E7DN63 (Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 1.8e-258
Identity = 436/784 (55.61%), Postives = 533/784 (67.98%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MW+LK+ EG N PYL+S+NN++GRQTW+F+P+ GT EERA++EEARQ +++NR+KV  SS
Sbjct: 1   MWKLKIAEGQNGPYLYSTNNYVGRQTWEFDPNGGTIEERAKIEEARQQFWNNRYKVKPSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEA-IINKETASIALRRATKFFA 120
           DL W              R+QFL E+NFKQ IP VKVEE   I+ E A+IAL RA  FF+
Sbjct: 61  DLLW--------------RIQFLGEKNFKQKIPAVKVEEGEEISHEVATIALHRAVNFFS 120

Query: 121 ALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWG 180
           ALQ+  GHWPAEN+GP+F+ PPLV  +YITGHL  +F  EHRKEILRY YCHQNEDGGWG
Sbjct: 121 ALQATDGHWPAENAGPLFFLPPLVMCMYITGHLNTVFPAEHRKEILRYIYCHQNEDGGWG 180

Query: 181 LHIEGQSCMLCTVFNYIQLRLLGEEPD---KDECFRARKWILDHGGAIYIPSWGKIWLSV 240
           LHIEG S M CT  +YI +R+LGE PD    + C RARKWILDHG    IPSWGK WLS 
Sbjct: 181 LHIEGHSTMFCTALSYICMRILGEGPDGGVNNACARARKWILDHGSVTAIPSWGKTWLS- 240

Query: 241 STILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLI 300
             ILGV+EW G NPMPPEFW+L   LP  P  ++CY R+  +PMSYL+GKRFVGP+TPLI
Sbjct: 241 --ILGVFEWIGTNPMPPEFWILPSFLPVHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLI 300

Query: 301 LQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTI 360
           LQLR+E+Y +PY+ I W   RH CAKED  +   L Q L WD+L    EP+L  W F  +
Sbjct: 301 LQLREELYDRPYDEINWKKVRHVCAKEDLYYPHPLVQDLMWDSLYICTEPLLTRWPFNKL 360

Query: 361 RNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL-------------------------- 420
           RN+AL++  +HI YED NS YITIGC+EKV  +L                          
Sbjct: 361 RNKALEVTMKHIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKKHLARIPDYLWVA 420

Query: 421 -----LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSMY 480
                + SF SQ WD  FAIQA+LA+ ++ E ++TL+KGHDFIKQSQV  NPS DF+ MY
Sbjct: 421 EDGMKMQSFGSQEWDTGFAIQALLASEMNDEIADTLRKGHDFIKQSQVTNNPSGDFKGMY 480

Query: 481 RHISKGSWTFSDQDHGWQVCDDTAE---------------------------------NL 540
           RHISKGSWTFSDQDHGWQV D TAE                                 +L
Sbjct: 481 RHISKGSWTFSDQDHGWQVSDCTAEALKCCLLLSTMPRELVGQAMEPGRLYDSVNVVLSL 540

Query: 541 LAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSHR 600
            +KNGG++AWEP  G   + E LNP EFF   ++E EYVECT +SIQAL+LFKKL+P HR
Sbjct: 541 QSKNGGLAAWEP-AGASEYLELLNPTEFFADIVIEHEYVECTASSIQALVLFKKLYPGHR 600

Query: 601 KKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQRG 660
            KEI  F+  AVKYLE+ Q+ DGSWYGNWG+C  Y ++FA+ GLVA+G +Y NS+ +++G
Sbjct: 601 TKEINIFIDNAVKYLEDVQMPDGSWYGNWGVCFTYGSWFALGGLVAAGKSYNNSAAVRKG 660

Query: 661 VEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPLH 716
           VEFLL+ Q  DGGWGES+ SC +K Y  L  N SNLVQT++AL+ LI S Q  RDP PLH
Sbjct: 661 VEFLLRTQRSDGGWGESYRSCPDKVYRELETNDSNLVQTAWALMGLIHSGQADRDPKPLH 720

BLAST of CmoCh05G009430 vs. ExPASy Swiss-Prot
Match: A8CDT2 (Beta-amyrin synthase OS=Bruguiera gymnorhiza OX=39984 GN=BAS PE=1 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 3.1e-258
Identity = 433/785 (55.16%), Postives = 532/785 (67.77%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWR+K+ EG  DPYL+S+NN++GRQTW+F+PD GTPEERAEVEEARQN+Y NR++V    
Sbjct: 1   MWRIKIAEGGKDPYLYSTNNYVGRQTWEFDPDAGTPEERAEVEEARQNFYKNRYQVKPCG 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEAI-INKETASIALRRATKFFA 120
           DL W              R+QFL E+NF+QTIP V++EE   I  E A+ ALRR  +FF+
Sbjct: 61  DLLW--------------RLQFLGEKNFEQTIPQVRIEEGEGITYEKATRALRRTVQFFS 120

Query: 121 ALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWG 180
           ALQ+  GHWPAE +GP+F+ PPLV  +YITGHL  +F  EHRKEILRY Y HQNEDGGWG
Sbjct: 121 ALQASDGHWPAEIAGPLFFLPPLVMCVYITGHLDAVFPAEHRKEILRYIYYHQNEDGGWG 180

Query: 181 LHIEGQSCMLCTVFNYIQLRLLGEEPD---KDECFRARKWILDHGGAIYIPSWGKIWLSV 240
           LHIEG S M CT  NYI +R++GE P+    D C RARKWI DHG    IPSWGK WLS 
Sbjct: 181 LHIEGHSTMFCTALNYICMRIIGEGPNGGQDDACARARKWIHDHGSVTNIPSWGKTWLS- 240

Query: 241 STILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLI 300
             ILGVY+W G+NPMPPEFW+L   LP  P  ++CY R+  +PMSYL+GKRFVGP+TPLI
Sbjct: 241 --ILGVYDWSGSNPMPPEFWMLPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLI 300

Query: 301 LQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAF-KT 360
            QLR+E++TQPY+ I W  TRH CA ED  +     Q L WD L  F EP+L  W   + 
Sbjct: 301 QQLREELFTQPYDQINWKKTRHQCAPEDLYYPHPFVQDLIWDCLYIFTEPLLTRWPLNEI 360

Query: 361 IRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------- 420
           IR +AL++  +HI YED +S YITIGC+EKV  +L                         
Sbjct: 361 IRKKALEVTMKHIHYEDESSRYITIGCVEKVLCMLACWVEDPNGDYFKKHLARIPDYIWV 420

Query: 421 ------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSM 480
                 + SF SQ WD  FAIQA+LATNL  E  + L++GHDFIK+SQVR+NPS DF+SM
Sbjct: 421 AEDGMKMQSFGSQEWDTGFAIQALLATNLTDEIGDVLRRGHDFIKKSQVRDNPSGDFKSM 480

Query: 481 YRHISKGSWTFSDQDHGWQVCDDTAE---------------------------------N 540
           YRHISKGSWTFSDQDHGWQV D TAE                                 +
Sbjct: 481 YRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMPPEIVGEHMVPERLYDSVNVLLS 540

Query: 541 LLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSH 600
           L +KNGG+SAWEP  G   W E LNP EFF   ++E EYVECT ++I AL+LFKKL+P H
Sbjct: 541 LQSKNGGLSAWEP-AGAQEWLELLNPTEFFADIVIEHEYVECTSSAIHALVLFKKLYPGH 600

Query: 601 RKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQR 660
           RKKEI+ F+  AV+YLE  Q  DG WYGNWG+C  Y T+FA+ GL A+G TY N   +++
Sbjct: 601 RKKEIDNFIVNAVRYLESIQTSDGGWYGNWGVCFTYGTWFALGGLAAAGKTYNNCLAMRK 660

Query: 661 GVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPL 716
            V+FLL+IQ  +GGWGES++SC EK+Y+PL GN SNLV T++AL+ALI + Q  RDPTPL
Sbjct: 661 AVDFLLRIQRDNGGWGESYLSCPEKRYVPLEGNRSNLVHTAWALMALIHAGQMDRDPTPL 720

BLAST of CmoCh05G009430 vs. ExPASy Swiss-Prot
Match: O82146 (Beta-amyrin synthase 2 OS=Panax ginseng OX=4054 GN=OSCPNY2 PE=2 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 5.3e-258
Identity = 435/784 (55.48%), Postives = 531/784 (67.73%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRL   +G NDPYL+S+NNF+GRQTW+F+PD GTP ERAEVEEAR ++++NR++V  SS
Sbjct: 1   MWRLMTAKGGNDPYLYSTNNFIGRQTWEFDPDYGTPAERAEVEEARLHFWNNRYQVKPSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEA-IINKETASIALRRATKFFA 120
           D+ W              RMQFL+E+NFKQ IP VKVE+   I  E A+  LRRA  +F+
Sbjct: 61  DVLW--------------RMQFLKEKNFKQIIPQVKVEDGEEITYEAATTTLRRAVHYFS 120

Query: 121 ALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWG 180
           ALQ+  GHWPAEN+GP+F+ PPLV  LYITGHL  +F  EHR EILRY YCHQN+DGGWG
Sbjct: 121 ALQADDGHWPAENAGPLFFLPPLVMCLYITGHLNTVFPAEHRIEILRYIYCHQNDDGGWG 180

Query: 181 LHIEGQSCMLCTVFNYIQLRLLGEEPDKDE---CFRARKWILDHGGAIYIPSWGKIWLSV 240
           LHIEG S M CT  +YI +R+LGE  D  E   C RARKWILDHG    IPSWGK WLS 
Sbjct: 181 LHIEGHSTMFCTALSYICMRILGEGRDGGENNACARARKWILDHGSVTAIPSWGKTWLS- 240

Query: 241 STILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLI 300
             ILG+++W G+NPMPPEFW+L   LP  P  ++CY R+  +PMSYL+GKRFVGP+TPLI
Sbjct: 241 --ILGLFDWSGSNPMPPEFWILPPFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLI 300

Query: 301 LQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTI 360
           LQLR+E+Y Q Y+ I W   RH CAKED  +   L Q L WD+L  F EP L  W F  +
Sbjct: 301 LQLREELYAQAYDEINWRKVRHNCAKEDLYYPHPLIQDLMWDSLYIFTEPFLTRWPFNKL 360

Query: 361 RNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL-------------------------- 420
           R +ALQ   +HI YED NS YITIGC+EKV  +L                          
Sbjct: 361 REKALQTTMKHIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKQHLARIPDYIWVA 420

Query: 421 -----LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSMY 480
                + SF SQ WD  FAIQA+LA++L  E   TL KGHDFIK+SQV+ENPS DF+SM+
Sbjct: 421 EDGMKMQSFGSQEWDTGFAIQALLASDLIDEIRPTLMKGHDFIKKSQVKENPSGDFKSMH 480

Query: 481 RHISKGSWTFSDQDHGWQVCDDTAE---------------------------------NL 540
           RHISKGSWTFSDQDHGWQV D TAE                                 +L
Sbjct: 481 RHISKGSWTFSDQDHGWQVSDCTAEALKCCLLFSRMPTEIVGDKMEDNQLFDAVNMLLSL 540

Query: 541 LAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSHR 600
            +KNGG++AWEP G    W E LNP EFFE  ++E EYVECT ++IQA+++FKKL+P HR
Sbjct: 541 QSKNGGLAAWEPAGS-SEWLELLNPTEFFEDIVIEHEYVECTSSAIQAMVMFKKLYPGHR 600

Query: 601 KKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQRG 660
           KKEIE  ++ AV+YLE+ Q+ DGSWYGNWG+C  Y T+FA+ GL A+G TY N  TL + 
Sbjct: 601 KKEIEVSITNAVQYLEDIQMPDGSWYGNWGVCFTYGTWFAMGGLTAAGKTYNNCQTLHKA 660

Query: 661 VEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPLH 716
           V+FL+K Q  DGGWGES++SC  K+Y PL GN SNLV TS+A++ LI S+Q +RDPTPLH
Sbjct: 661 VDFLIKSQRSDGGWGESYLSCPNKEYTPLEGNRSNLVHTSWAMMGLIHSRQAERDPTPLH 720

BLAST of CmoCh05G009430 vs. ExPASy TrEMBL
Match: A0A6J1G3T6 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 1273.1 bits (3293), Expect = 0.0e+00
Identity = 624/788 (79.19%), Postives = 656/788 (83.25%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLKLGEGANDPYLFSSNNF+GRQTWDFE DEGTP++RAEVE+ARQN+Y NRFKV CSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEE--------AIINKETASIALR 120
           + FWKF              Q LRERNFKQTIP+VKVEE        A+I KET +IALR
Sbjct: 61  NFFWKF--------------QVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALR 120

Query: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180
           RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ
Sbjct: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180

Query: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240
           NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI
Sbjct: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240

Query: 241 WLSVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPL 300
           WLS   ILGVYEWEGANPMPPEFWLLGKLLPFIP++LFCYSRLTLLPMSYLFGKRFVG L
Sbjct: 241 WLS---ILGVYEWEGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTL 300

Query: 301 TPLILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA 360
           TPLILQLRQEIYTQPYNHIKW+PTRHYCAKEDKCFERSLFQKL WDA+QYFGEPILNS A
Sbjct: 301 TPLILQLRQEIYTQPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSA 360

Query: 361 FKTIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL---------------------- 420
           FKTIRNRALQ+AK HIDYEDHNSHYITIGC+EK  F L                      
Sbjct: 361 FKTIRNRALQVAKCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDY 420

Query: 421 ---------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDF 480
                    + SF SQSWDVAFAIQAILATNLHHEFSETLKKGHDF+KQSQVRENP  DF
Sbjct: 421 LWIAEDGMKMQSFGSQSWDVAFAIQAILATNLHHEFSETLKKGHDFVKQSQVRENPCGDF 480

Query: 481 RSMYRHISKGSWTFSDQDHGWQVCDDTAENLL---------------------------- 540
           RSMYRHISKGSWTFSDQDHGWQ+ D T ENLL                            
Sbjct: 481 RSMYRHISKGSWTFSDQDHGWQLSDCTGENLLCCLKFSTMPSNIVGDPMEPQWFFEAVNV 540

Query: 541 -----AKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLF 600
                AKNGGV AWEPTG VPSWFE LNPVEF EYT+LELEYVECT +S+QAL+LF KLF
Sbjct: 541 LLSLQAKNGGVPAWEPTGVVPSWFELLNPVEFLEYTVLELEYVECTSSSVQALVLFTKLF 600

Query: 601 PSHRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSST 660
           P+HRKKEIETFLSKAV Y+EETQ EDGSWYGNWGICHIYATYFAIKGLVA+GNTY NSST
Sbjct: 601 PNHRKKEIETFLSKAVIYMEETQKEDGSWYGNWGICHIYATYFAIKGLVATGNTYHNSST 660

Query: 661 LQRGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDP 716
           +++GV+FLLKIQCPDGGWGESHISC +KKYIPLP NSSNLVQTSFAL+ALI SQQEKRDP
Sbjct: 661 IRKGVDFLLKIQCPDGGWGESHISCTQKKYIPLPENSSNLVQTSFALMALIHSQQEKRDP 720

BLAST of CmoCh05G009430 vs. ExPASy TrEMBL
Match: A0A6J1G4X9 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450754 PE=3 SV=1)

HSP 1 Score: 1272.7 bits (3292), Expect = 0.0e+00
Identity = 617/780 (79.10%), Postives = 655/780 (83.97%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRL +GEGA+DPYLFSSNNF+GRQTWDFEPD+GTPEERAEVEEARQNYYHNRFKVPC+S
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEAIINKETASIALRRATKFFAA 120
           DLFW+F              QFLRERNFKQTIPIV+VE+ +I+KETASIALRRAT FFAA
Sbjct: 61  DLFWRF--------------QFLRERNFKQTIPIVRVED-VIDKETASIALRRATNFFAA 120

Query: 121 LQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGL 180
           LQS HGHWPAEN+GPMFYFPPLVFSLYITGHL IIFSE HRKEILRY+YCHQNEDGGWGL
Sbjct: 121 LQSPHGHWPAENAGPMFYFPPLVFSLYITGHLRIIFSEHHRKEILRYSYCHQNEDGGWGL 180

Query: 181 HIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSVSTIL 240
           HI GQSCM CTVFNYIQLRLLGEEPDKDECFRARKWILDHGGA+YIPSWGKIWLS   IL
Sbjct: 181 HIVGQSCMFCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGALYIPSWGKIWLS---IL 240

Query: 241 GVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLR 300
           GVYEWEG NPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYL+GKRFVGPLTPLILQLR
Sbjct: 241 GVYEWEGTNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLR 300

Query: 301 QEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRA 360
           QEIYTQPYNHIKW+PTRHYCAKEDKCFER   QKLAWDALQYFGEPILNSWAFKTIRNRA
Sbjct: 301 QEIYTQPYNHIKWSPTRHYCAKEDKCFERLYXQKLAWDALQYFGEPILNSWAFKTIRNRA 360

Query: 361 LQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------------ 420
           LQIAKRHIDYEDHNSHYITIGCIEK  F L                              
Sbjct: 361 LQIAKRHIDYEDHNSHYITIGCIEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGM 420

Query: 421 -LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSMYRHIS 480
            + S+ SQSWDVAF+IQA+LATNLHHEFSETLKKGHDFIKQSQVRENPS DF+SMYR+IS
Sbjct: 421 KMQSYGSQSWDVAFSIQAVLATNLHHEFSETLKKGHDFIKQSQVRENPSGDFQSMYRYIS 480

Query: 481 KGSWTFSDQDHGWQVCDDTAENLL---------------------------------AKN 540
           KGSWTFSD+DHGWQ+ D TAENLL                                 AKN
Sbjct: 481 KGSWTFSDRDHGWQISDSTAENLLCCLIFSTMPSDIVGDPMELQWFFEAVNIILSLQAKN 540

Query: 541 GGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSHRKKEI 600
           GGVSAWEPTGGVPSWFE LNPVEF EYTILELEYVECT ++IQAL+LF+KLFP+HRKKEI
Sbjct: 541 GGVSAWEPTGGVPSWFELLNPVEFLEYTILELEYVECTSSAIQALVLFRKLFPNHRKKEI 600

Query: 601 ETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQRGVEFL 660
           +TFLSK VKYLEETQ EDGSW+G WGIC+ Y+TYFA+KGLV +GNTY NSSTL+RGVEFL
Sbjct: 601 KTFLSKGVKYLEETQKEDGSWHGYWGICYTYSTYFAVKGLVTTGNTYNNSSTLRRGVEFL 660

Query: 661 LKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPLHRGAK 716
           LKIQCPDGGWGESHISC +KKYIPLPGNSSNLVQTSFAL+ALI SQQEKRDPTPLHR AK
Sbjct: 661 LKIQCPDGGWGESHISCMQKKYIPLPGNSSNLVQTSFALMALIHSQQEKRDPTPLHRAAK 720

BLAST of CmoCh05G009430 vs. ExPASy TrEMBL
Match: A0A6J1G3X4 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 618/788 (78.43%), Postives = 653/788 (82.87%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLKLGEGANDPYLFSSNNF+GRQTWDFE DEGTP++RAEVE+ARQN+Y NRFKV CSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEE--------AIINKETASIALR 120
           + FWKF              Q LRERNFKQTIP+VKVEE        A+I KET +IALR
Sbjct: 61  NFFWKF--------------QVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALR 120

Query: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180
           RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ
Sbjct: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180

Query: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240
           NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI
Sbjct: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240

Query: 241 WLSVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPL 300
           WLS   ILGVYEWEGANPMPPEFWLLGKLLPFIP++LFCYSRLTLLPMSYLFGKRFVG L
Sbjct: 241 WLS---ILGVYEWEGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTL 300

Query: 301 TPLILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA 360
           TPLILQLRQEIYTQPYNHIKW+PTRHYCAKEDKCFERSLFQKL WDA+QYFGEPILNS A
Sbjct: 301 TPLILQLRQEIYTQPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSA 360

Query: 361 FKTIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL---------------------- 420
           FKTIRNRALQ+AK HIDYEDHNSHYITIGC+EK  F L                      
Sbjct: 361 FKTIRNRALQVAKCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDY 420

Query: 421 ---------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDF 480
                    + SF SQSWDVAFAIQAILATNLHHEFSETLKKGHDF+KQSQVRENP  DF
Sbjct: 421 LWIAEDGMKMQSFGSQSWDVAFAIQAILATNLHHEFSETLKKGHDFVKQSQVRENPCGDF 480

Query: 481 RSMYRHISKGSWTFSDQDHGWQVCDDTAENLL---------------------------- 540
           RSMYRHISKGSWTFSDQDHGWQ+ D T ENLL                            
Sbjct: 481 RSMYRHISKGSWTFSDQDHGWQLSDCTGENLLCCLKFSTMPSNIVGDPMEPQWFFEAVNV 540

Query: 541 -----AKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLF 600
                AKNGGV AWEPTG VPSWFE LNPVEF EYT+LELEYVECT +S+QAL+LF KLF
Sbjct: 541 LLSLQAKNGGVPAWEPTGVVPSWFELLNPVEFLEYTVLELEYVECTSSSVQALVLFTKLF 600

Query: 601 PSHRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSST 660
           P+HRKKEIETFLSKAV Y+EETQ EDGSWYGNWGICHIYATYFAIKGLVA+GNTY NSST
Sbjct: 601 PNHRKKEIETFLSKAVIYMEETQKEDGSWYGNWGICHIYATYFAIKGLVATGNTYHNSST 660

Query: 661 LQRGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDP 716
           +++GV+FLLKIQCPDGGWGESHISC +KKYIPLP NSSNLVQTSFAL+ALI SQQEKRDP
Sbjct: 661 IRKGVDFLLKIQCPDGGWGESHISCTQKKYIPLPENSSNLVQTSFALMALIHSQQEKRDP 720

BLAST of CmoCh05G009430 vs. ExPASy TrEMBL
Match: A0A6J1G4T2 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450753 PE=3 SV=1)

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 617/787 (78.40%), Postives = 653/787 (82.97%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLKLGEG NDPYLFSSNNFMGRQTWDFEPD+GTP+ERAEVEEARQNYY NRFKV CSS
Sbjct: 1   MWRLKLGEGGNDPYLFSSNNFMGRQTWDFEPDDGTPQERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEE--------AIINKETASIALR 120
           DLFWKF              QFLRERNFKQTIPI++VE+        AII KET +IALR
Sbjct: 61  DLFWKF--------------QFLRERNFKQTIPIMRVEDVVDEDKKVAIIKKETVTIALR 120

Query: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180
           RAT FFAALQS HGHWPAEN+GPMFYFPPLVFSLYITGHL I+FSEEHRKEILRYAYCHQ
Sbjct: 121 RATNFFAALQSPHGHWPAENAGPMFYFPPLVFSLYITGHLHIVFSEEHRKEILRYAYCHQ 180

Query: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240
           NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI
Sbjct: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240

Query: 241 WLSVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPL 300
           WLS   ILGVYEWEGANPMPPEFWLLGKLLPFIPR LFC+SRLTLLPM+YLFGKRFVG L
Sbjct: 241 WLS---ILGVYEWEGANPMPPEFWLLGKLLPFIPRNLFCHSRLTLLPMTYLFGKRFVGSL 300

Query: 301 TPLILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA 360
           TPLILQLRQEIYTQPYNHIKW+PTRHYCAKEDKCFERSLFQKLAWDALQY GEPILNSWA
Sbjct: 301 TPLILQLRQEIYTQPYNHIKWSPTRHYCAKEDKCFERSLFQKLAWDALQYIGEPILNSWA 360

Query: 361 FKTIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL---------------------- 420
           FKTIRNRALQIAK HIDYEDH+SHYITIGC+EK    L                      
Sbjct: 361 FKTIRNRALQIAKCHIDYEDHHSHYITIGCVEKPLLTLACWVDDPNGEAYRKHVARIKDY 420

Query: 421 ---------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDF 480
                    + S+ SQSWDVAFAIQA+LATNL+HE SETLKKGH FIKQSQVRENPS DF
Sbjct: 421 LWMGEDGMKMQSYGSQSWDVAFAIQAMLATNLYHENSETLKKGHTFIKQSQVRENPSGDF 480

Query: 481 RSMYRHISKGSWTFSDQDHGWQVCDDTAENLL---------------------------- 540
           R+MYRHISKGSWTFSD+DHGWQ+ D TAENLL                            
Sbjct: 481 RNMYRHISKGSWTFSDRDHGWQISDCTAENLLCCLRFSTMPSNIVGDPMEPQWFFEAVNF 540

Query: 541 -----AKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLF 600
                AKNGGVSAWEP G VPSWFE LNPVEF EYT+LE EYVECT +SIQAL+LF KLF
Sbjct: 541 ILSLQAKNGGVSAWEPNGAVPSWFELLNPVEFLEYTVLEREYVECTSSSIQALVLFTKLF 600

Query: 601 PSHRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSST 660
           P+HRKKEIETFLSKA+KYLEETQ EDGSW+GNWG+CHIYATYFAIKGLVA+GNTY NSST
Sbjct: 601 PNHRKKEIETFLSKAIKYLEETQKEDGSWFGNWGVCHIYATYFAIKGLVATGNTYHNSST 660

Query: 661 LQRGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDP 715
           +++GVEFLLKIQCPDGGWGESH+SC +KKYIPLP NSSNLVQTSFAL+ALI SQQ+KRDP
Sbjct: 661 IRKGVEFLLKIQCPDGGWGESHVSCMQKKYIPLPRNSSNLVQTSFALMALIHSQQKKRDP 720

BLAST of CmoCh05G009430 vs. ExPASy TrEMBL
Match: A0A6J1KCM3 (Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493703 PE=3 SV=1)

HSP 1 Score: 1262.3 bits (3265), Expect = 0.0e+00
Identity = 618/787 (78.53%), Postives = 654/787 (83.10%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLKLGEGANDPYLFSSNNF+GRQTWDFEP EGTPEERAEVEEARQNYYHNRFKVPCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPHEGTPEERAEVEEARQNYYHNRFKVPCSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEE--------AIINKETASIALR 120
           DLFWKF              QFLR+RNFKQTIPI KVEE        AIINKETAS ALR
Sbjct: 61  DLFWKF--------------QFLRDRNFKQTIPIAKVEEGIDGDEKVAIINKETASNALR 120

Query: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180
           RAT FFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLG+IFS+ HR EILRY+YCHQ
Sbjct: 121 RATLFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGVIFSQHHRNEILRYSYCHQ 180

Query: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240
           NEDGGWGLHI+GQSCMLCTVFNY+QLRLLGE+PDKDECFRARKWILDHGGAIYIPSWGKI
Sbjct: 181 NEDGGWGLHIQGQSCMLCTVFNYLQLRLLGEQPDKDECFRARKWILDHGGAIYIPSWGKI 240

Query: 241 WLSVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPL 300
           WLS   ILGVY+WEGANPMPPEFWLLGKLLPFIP++LFCYSR+TLLPMSYLFGKRFVGPL
Sbjct: 241 WLS---ILGVYQWEGANPMPPEFWLLGKLLPFIPQSLFCYSRMTLLPMSYLFGKRFVGPL 300

Query: 301 TPLILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA 360
           TPLILQLRQEIYTQ YN+IKW+PTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA
Sbjct: 301 TPLILQLRQEIYTQSYNNIKWSPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA 360

Query: 361 FKTIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL---------------------- 420
           FKTIRNRALQ+AK HIDYEDHNSHYITIG  EK  F L                      
Sbjct: 361 FKTIRNRALQVAKYHIDYEDHNSHYITIGGGEKPLFTLACWVDDPNGEAYKKHLARIKDY 420

Query: 421 ---------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDF 480
                    + SF SQSWDVAFAIQAILATNLHHEFSETLK+GHDFIKQSQV+ENPS DF
Sbjct: 421 LWVGEDGMKMQSFGSQSWDVAFAIQAILATNLHHEFSETLKRGHDFIKQSQVKENPSGDF 480

Query: 481 RSMYRHISKGSWTFSDQDHGWQVCDDTAENLL---------------------------- 540
           RSMYRHISKGSWTFS++DHGWQV D TAENLL                            
Sbjct: 481 RSMYRHISKGSWTFSNRDHGWQVSDCTAENLLCCLRFSTMPSNIVGDPMEPQWFFEAVNV 540

Query: 541 -----AKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLF 600
                AKNGGV AWEPTG VPSWFE LNPVEF EYT+LELEYVECT +SIQAL+LF KLF
Sbjct: 541 ILSLQAKNGGVPAWEPTGVVPSWFELLNPVEFLEYTVLELEYVECTSSSIQALVLFTKLF 600

Query: 601 PSHRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSST 660
           P+HRKKEIETFLSKA KY+EETQ EDGSWYGNWGICHIYATYFAIKGLVA+GNTY NSST
Sbjct: 601 PNHRKKEIETFLSKAEKYIEETQKEDGSWYGNWGICHIYATYFAIKGLVATGNTYHNSST 660

Query: 661 LQRGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDP 715
           ++RGVEFLLKIQC DGGWGESH+SC +KKYIPLP NSSNLVQTSFAL+ALI S QEKRDP
Sbjct: 661 IRRGVEFLLKIQCSDGGWGESHLSCTQKKYIPLPRNSSNLVQTSFALMALIHSHQEKRDP 720

BLAST of CmoCh05G009430 vs. NCBI nr
Match: XP_022946462.1 (beta-amyrin synthase-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 1273.1 bits (3293), Expect = 0.0e+00
Identity = 624/788 (79.19%), Postives = 656/788 (83.25%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLKLGEGANDPYLFSSNNF+GRQTWDFE DEGTP++RAEVE+ARQN+Y NRFKV CSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEE--------AIINKETASIALR 120
           + FWKF              Q LRERNFKQTIP+VKVEE        A+I KET +IALR
Sbjct: 61  NFFWKF--------------QVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALR 120

Query: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180
           RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ
Sbjct: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180

Query: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240
           NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI
Sbjct: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240

Query: 241 WLSVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPL 300
           WLS   ILGVYEWEGANPMPPEFWLLGKLLPFIP++LFCYSRLTLLPMSYLFGKRFVG L
Sbjct: 241 WLS---ILGVYEWEGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTL 300

Query: 301 TPLILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA 360
           TPLILQLRQEIYTQPYNHIKW+PTRHYCAKEDKCFERSLFQKL WDA+QYFGEPILNS A
Sbjct: 301 TPLILQLRQEIYTQPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSA 360

Query: 361 FKTIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL---------------------- 420
           FKTIRNRALQ+AK HIDYEDHNSHYITIGC+EK  F L                      
Sbjct: 361 FKTIRNRALQVAKCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDY 420

Query: 421 ---------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDF 480
                    + SF SQSWDVAFAIQAILATNLHHEFSETLKKGHDF+KQSQVRENP  DF
Sbjct: 421 LWIAEDGMKMQSFGSQSWDVAFAIQAILATNLHHEFSETLKKGHDFVKQSQVRENPCGDF 480

Query: 481 RSMYRHISKGSWTFSDQDHGWQVCDDTAENLL---------------------------- 540
           RSMYRHISKGSWTFSDQDHGWQ+ D T ENLL                            
Sbjct: 481 RSMYRHISKGSWTFSDQDHGWQLSDCTGENLLCCLKFSTMPSNIVGDPMEPQWFFEAVNV 540

Query: 541 -----AKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLF 600
                AKNGGV AWEPTG VPSWFE LNPVEF EYT+LELEYVECT +S+QAL+LF KLF
Sbjct: 541 LLSLQAKNGGVPAWEPTGVVPSWFELLNPVEFLEYTVLELEYVECTSSSVQALVLFTKLF 600

Query: 601 PSHRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSST 660
           P+HRKKEIETFLSKAV Y+EETQ EDGSWYGNWGICHIYATYFAIKGLVA+GNTY NSST
Sbjct: 601 PNHRKKEIETFLSKAVIYMEETQKEDGSWYGNWGICHIYATYFAIKGLVATGNTYHNSST 660

Query: 661 LQRGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDP 716
           +++GV+FLLKIQCPDGGWGESHISC +KKYIPLP NSSNLVQTSFAL+ALI SQQEKRDP
Sbjct: 661 IRKGVDFLLKIQCPDGGWGESHISCTQKKYIPLPENSSNLVQTSFALMALIHSQQEKRDP 720

BLAST of CmoCh05G009430 vs. NCBI nr
Match: XP_022946794.1 (LOW QUALITY PROTEIN: beta-amyrin synthase-like [Cucurbita moschata])

HSP 1 Score: 1272.7 bits (3292), Expect = 0.0e+00
Identity = 617/780 (79.10%), Postives = 655/780 (83.97%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRL +GEGA+DPYLFSSNNF+GRQTWDFEPD+GTPEERAEVEEARQNYYHNRFKVPC+S
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEAIINKETASIALRRATKFFAA 120
           DLFW+F              QFLRERNFKQTIPIV+VE+ +I+KETASIALRRAT FFAA
Sbjct: 61  DLFWRF--------------QFLRERNFKQTIPIVRVED-VIDKETASIALRRATNFFAA 120

Query: 121 LQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGL 180
           LQS HGHWPAEN+GPMFYFPPLVFSLYITGHL IIFSE HRKEILRY+YCHQNEDGGWGL
Sbjct: 121 LQSPHGHWPAENAGPMFYFPPLVFSLYITGHLRIIFSEHHRKEILRYSYCHQNEDGGWGL 180

Query: 181 HIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSVSTIL 240
           HI GQSCM CTVFNYIQLRLLGEEPDKDECFRARKWILDHGGA+YIPSWGKIWLS   IL
Sbjct: 181 HIVGQSCMFCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGALYIPSWGKIWLS---IL 240

Query: 241 GVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLR 300
           GVYEWEG NPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYL+GKRFVGPLTPLILQLR
Sbjct: 241 GVYEWEGTNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLR 300

Query: 301 QEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRA 360
           QEIYTQPYNHIKW+PTRHYCAKEDKCFER   QKLAWDALQYFGEPILNSWAFKTIRNRA
Sbjct: 301 QEIYTQPYNHIKWSPTRHYCAKEDKCFERLYXQKLAWDALQYFGEPILNSWAFKTIRNRA 360

Query: 361 LQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------------ 420
           LQIAKRHIDYEDHNSHYITIGCIEK  F L                              
Sbjct: 361 LQIAKRHIDYEDHNSHYITIGCIEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGM 420

Query: 421 -LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSMYRHIS 480
            + S+ SQSWDVAF+IQA+LATNLHHEFSETLKKGHDFIKQSQVRENPS DF+SMYR+IS
Sbjct: 421 KMQSYGSQSWDVAFSIQAVLATNLHHEFSETLKKGHDFIKQSQVRENPSGDFQSMYRYIS 480

Query: 481 KGSWTFSDQDHGWQVCDDTAENLL---------------------------------AKN 540
           KGSWTFSD+DHGWQ+ D TAENLL                                 AKN
Sbjct: 481 KGSWTFSDRDHGWQISDSTAENLLCCLIFSTMPSDIVGDPMELQWFFEAVNIILSLQAKN 540

Query: 541 GGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSHRKKEI 600
           GGVSAWEPTGGVPSWFE LNPVEF EYTILELEYVECT ++IQAL+LF+KLFP+HRKKEI
Sbjct: 541 GGVSAWEPTGGVPSWFELLNPVEFLEYTILELEYVECTSSAIQALVLFRKLFPNHRKKEI 600

Query: 601 ETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQRGVEFL 660
           +TFLSK VKYLEETQ EDGSW+G WGIC+ Y+TYFA+KGLV +GNTY NSSTL+RGVEFL
Sbjct: 601 KTFLSKGVKYLEETQKEDGSWHGYWGICYTYSTYFAVKGLVTTGNTYNNSSTLRRGVEFL 660

Query: 661 LKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPLHRGAK 716
           LKIQCPDGGWGESHISC +KKYIPLPGNSSNLVQTSFAL+ALI SQQEKRDPTPLHR AK
Sbjct: 661 LKIQCPDGGWGESHISCMQKKYIPLPGNSSNLVQTSFALMALIHSQQEKRDPTPLHRAAK 720

BLAST of CmoCh05G009430 vs. NCBI nr
Match: XP_023545920.1 (LOW QUALITY PROTEIN: beta-Amyrin Synthase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1269.6 bits (3284), Expect = 0.0e+00
Identity = 621/780 (79.62%), Postives = 652/780 (83.59%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLKLGEGANDPYLFSSNNF+GRQTWDFEPDEGTPEERA+VEEARQ+YYHNRF+V C+S
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPDEGTPEERAQVEEARQSYYHNRFRVQCNS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEAIINKETASIALRRATKFFAA 120
           DLFWKF              QFLRERNFKQTIPIVKVEEAIINKET SIALRRATKFFAA
Sbjct: 61  DLFWKF--------------QFLRERNFKQTIPIVKVEEAIINKETTSIALRRATKFFAA 120

Query: 121 LQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGL 180
           LQS HGHWPAEN+GPMFYFPPLVFSLYITGHL I+FSEEHRKEILRYAYCHQNEDGGWGL
Sbjct: 121 LQSPHGHWPAENAGPMFYFPPLVFSLYITGHLHIVFSEEHRKEILRYAYCHQNEDGGWGL 180

Query: 181 HIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSVSTIL 240
           HI GQSCMLCTVFNYIQLRLLGEE DKDEC RARKWILDHGGA+YIPSWGKIWLS   IL
Sbjct: 181 HIVGQSCMLCTVFNYIQLRLLGEELDKDECCRARKWILDHGGALYIPSWGKIWLS---IL 240

Query: 241 GVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLR 300
           GVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLR
Sbjct: 241 GVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLR 300

Query: 301 QEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRA 360
           QEIYTQPYN+IKW+PTRH+CAKEDKCFERSLFQKL WDA+QYFGEPILNSWAFKTIRNRA
Sbjct: 301 QEIYTQPYNNIKWSPTRHFCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNRA 360

Query: 361 LQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------------ 420
           LQ+AK H+DYEDHNSHYITIGC+EK  F L                              
Sbjct: 361 LQVAKCHMDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWIAEDGM 420

Query: 421 -LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSMYRHIS 480
            + SF SQSWDVA AI A+LATNL  EFSETLKKGHDFIKQSQVRENP  DFRSMYRHIS
Sbjct: 421 KMQSFGSQSWDVALAIXALLATNLRDEFSETLKKGHDFIKQSQVRENPCGDFRSMYRHIS 480

Query: 481 KGSWTFSDQDHGWQVCDDTAENLL---------------------------------AKN 540
           KGSWTFSD+DHGWQV D TAENLL                                 AKN
Sbjct: 481 KGSWTFSDRDHGWQVSDCTAENLLCCLIFSTMPSNIVGDPMEPQCFFEAVDIILSLQAKN 540

Query: 541 GGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSHRKKEI 600
           GGVSAWEPTG VPSWFE LNPVEFFEYT+LELEYVECT +SIQAL+LF KLFPSHRKKEI
Sbjct: 541 GGVSAWEPTGVVPSWFELLNPVEFFEYTVLELEYVECTSSSIQALVLFTKLFPSHRKKEI 600

Query: 601 ETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQRGVEFL 660
           E FLSKAVKYLEETQ EDGSWYGNWG+ HIYATYFAIKGL A+GNTY NSSTL+R VEFL
Sbjct: 601 EIFLSKAVKYLEETQKEDGSWYGNWGVFHIYATYFAIKGLTATGNTYNNSSTLRRAVEFL 660

Query: 661 LKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPLHRGAK 716
           LKIQCPDGGWGESHISC +KKY+PLP NSSNLVQTSFAL+ALI SQQEKRDPTPLH  AK
Sbjct: 661 LKIQCPDGGWGESHISCTQKKYLPLPRNSSNLVQTSFALMALIHSQQEKRDPTPLHGAAK 720

BLAST of CmoCh05G009430 vs. NCBI nr
Match: XP_022946460.1 (beta-amyrin synthase-like isoform X1 [Cucurbita moschata] >XP_022946461.1 beta-amyrin synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 618/788 (78.43%), Postives = 653/788 (82.87%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLKLGEGANDPYLFSSNNF+GRQTWDFE DEGTP++RAEVE+ARQN+Y NRFKV CSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEE--------AIINKETASIALR 120
           + FWKF              Q LRERNFKQTIP+VKVEE        A+I KET +IALR
Sbjct: 61  NFFWKF--------------QVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALR 120

Query: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180
           RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ
Sbjct: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180

Query: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240
           NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI
Sbjct: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240

Query: 241 WLSVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPL 300
           WLS   ILGVYEWEGANPMPPEFWLLGKLLPFIP++LFCYSRLTLLPMSYLFGKRFVG L
Sbjct: 241 WLS---ILGVYEWEGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTL 300

Query: 301 TPLILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA 360
           TPLILQLRQEIYTQPYNHIKW+PTRHYCAKEDKCFERSLFQKL WDA+QYFGEPILNS A
Sbjct: 301 TPLILQLRQEIYTQPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSA 360

Query: 361 FKTIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL---------------------- 420
           FKTIRNRALQ+AK HIDYEDHNSHYITIGC+EK  F L                      
Sbjct: 361 FKTIRNRALQVAKCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDY 420

Query: 421 ---------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDF 480
                    + SF SQSWDVAFAIQAILATNLHHEFSETLKKGHDF+KQSQVRENP  DF
Sbjct: 421 LWIAEDGMKMQSFGSQSWDVAFAIQAILATNLHHEFSETLKKGHDFVKQSQVRENPCGDF 480

Query: 481 RSMYRHISKGSWTFSDQDHGWQVCDDTAENLL---------------------------- 540
           RSMYRHISKGSWTFSDQDHGWQ+ D T ENLL                            
Sbjct: 481 RSMYRHISKGSWTFSDQDHGWQLSDCTGENLLCCLKFSTMPSNIVGDPMEPQWFFEAVNV 540

Query: 541 -----AKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLF 600
                AKNGGV AWEPTG VPSWFE LNPVEF EYT+LELEYVECT +S+QAL+LF KLF
Sbjct: 541 LLSLQAKNGGVPAWEPTGVVPSWFELLNPVEFLEYTVLELEYVECTSSSVQALVLFTKLF 600

Query: 601 PSHRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSST 660
           P+HRKKEIETFLSKAV Y+EETQ EDGSWYGNWGICHIYATYFAIKGLVA+GNTY NSST
Sbjct: 601 PNHRKKEIETFLSKAVIYMEETQKEDGSWYGNWGICHIYATYFAIKGLVATGNTYHNSST 660

Query: 661 LQRGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDP 716
           +++GV+FLLKIQCPDGGWGESHISC +KKYIPLP NSSNLVQTSFAL+ALI SQQEKRDP
Sbjct: 661 IRKGVDFLLKIQCPDGGWGESHISCTQKKYIPLPENSSNLVQTSFALMALIHSQQEKRDP 720

BLAST of CmoCh05G009430 vs. NCBI nr
Match: XP_022946788.1 (beta-amyrin synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 617/787 (78.40%), Postives = 653/787 (82.97%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60
           MWRLKLGEG NDPYLFSSNNFMGRQTWDFEPD+GTP+ERAEVEEARQNYY NRFKV CSS
Sbjct: 1   MWRLKLGEGGNDPYLFSSNNFMGRQTWDFEPDDGTPQERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  DLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEE--------AIINKETASIALR 120
           DLFWKF              QFLRERNFKQTIPI++VE+        AII KET +IALR
Sbjct: 61  DLFWKF--------------QFLRERNFKQTIPIMRVEDVVDEDKKVAIIKKETVTIALR 120

Query: 121 RATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQ 180
           RAT FFAALQS HGHWPAEN+GPMFYFPPLVFSLYITGHL I+FSEEHRKEILRYAYCHQ
Sbjct: 121 RATNFFAALQSPHGHWPAENAGPMFYFPPLVFSLYITGHLHIVFSEEHRKEILRYAYCHQ 180

Query: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240
           NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI
Sbjct: 181 NEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKI 240

Query: 241 WLSVSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPL 300
           WLS   ILGVYEWEGANPMPPEFWLLGKLLPFIPR LFC+SRLTLLPM+YLFGKRFVG L
Sbjct: 241 WLS---ILGVYEWEGANPMPPEFWLLGKLLPFIPRNLFCHSRLTLLPMTYLFGKRFVGSL 300

Query: 301 TPLILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWA 360
           TPLILQLRQEIYTQPYNHIKW+PTRHYCAKEDKCFERSLFQKLAWDALQY GEPILNSWA
Sbjct: 301 TPLILQLRQEIYTQPYNHIKWSPTRHYCAKEDKCFERSLFQKLAWDALQYIGEPILNSWA 360

Query: 361 FKTIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL---------------------- 420
           FKTIRNRALQIAK HIDYEDH+SHYITIGC+EK    L                      
Sbjct: 361 FKTIRNRALQIAKCHIDYEDHHSHYITIGCVEKPLLTLACWVDDPNGEAYRKHVARIKDY 420

Query: 421 ---------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDF 480
                    + S+ SQSWDVAFAIQA+LATNL+HE SETLKKGH FIKQSQVRENPS DF
Sbjct: 421 LWMGEDGMKMQSYGSQSWDVAFAIQAMLATNLYHENSETLKKGHTFIKQSQVRENPSGDF 480

Query: 481 RSMYRHISKGSWTFSDQDHGWQVCDDTAENLL---------------------------- 540
           R+MYRHISKGSWTFSD+DHGWQ+ D TAENLL                            
Sbjct: 481 RNMYRHISKGSWTFSDRDHGWQISDCTAENLLCCLRFSTMPSNIVGDPMEPQWFFEAVNF 540

Query: 541 -----AKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLF 600
                AKNGGVSAWEP G VPSWFE LNPVEF EYT+LE EYVECT +SIQAL+LF KLF
Sbjct: 541 ILSLQAKNGGVSAWEPNGAVPSWFELLNPVEFLEYTVLEREYVECTSSSIQALVLFTKLF 600

Query: 601 PSHRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSST 660
           P+HRKKEIETFLSKA+KYLEETQ EDGSW+GNWG+CHIYATYFAIKGLVA+GNTY NSST
Sbjct: 601 PNHRKKEIETFLSKAIKYLEETQKEDGSWFGNWGVCHIYATYFAIKGLVATGNTYHNSST 660

Query: 661 LQRGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDP 715
           +++GVEFLLKIQCPDGGWGESH+SC +KKYIPLP NSSNLVQTSFAL+ALI SQQ+KRDP
Sbjct: 661 IRKGVEFLLKIQCPDGGWGESHVSCMQKKYIPLPRNSSNLVQTSFALMALIHSQQKKRDP 720

BLAST of CmoCh05G009430 vs. TAIR 10
Match: AT1G78960.1 (lupeol synthase 2 )

HSP 1 Score: 862.4 bits (2227), Expect = 2.5e-250
Identity = 431/779 (55.33%), Postives = 521/779 (66.88%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCS 60
           MW+LK+GEG   DPYLFSSNNF+GRQTW+F+P  GTPEERA VE+AR+NY  NR +V   
Sbjct: 1   MWKLKIGEGNGEDPYLFSSNNFVGRQTWEFDPKAGTPEERAAVEDARRNYLDNRPRVKGC 60

Query: 61  SDLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEAI-INKETASIALRRATKFF 120
           SDL W              RMQFL+E  F+Q IP VK+++   I  + A+ ALRRA  F+
Sbjct: 61  SDLLW--------------RMQFLKEAKFEQVIPPVKIDDGEGITYKNATDALRRAVSFY 120

Query: 121 AALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGW 180
           +ALQS  GHWPAE +G +F+ PPLVF  YITGHL  IF  EHRKE+LR+ YCHQNEDGGW
Sbjct: 121 SALQSSDGHWPAEITGTLFFLPPLVFCFYITGHLEKIFDAEHRKEMLRHIYCHQNEDGGW 180

Query: 181 GLHIEGQSCMLCTVFNYIQLRLLGEEPD---KDECFRARKWILDHGGAIYIPSWGKIWLS 240
           GLHIEG+S M CTV NYI LR+LGE P+    + C RAR+WILDHGG  YIPSWGKIWLS
Sbjct: 181 GLHIEGKSVMFCTVLNYICLRMLGEGPNGGRNNACKRARQWILDHGGVTYIPSWGKIWLS 240

Query: 241 VSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPL 300
              ILG+Y+W G NPMPPE WLL    P       CY+R+  +PMSYL+GKRFVGPLTPL
Sbjct: 241 ---ILGIYDWSGTNPMPPEIWLLPSFFPIHLGKTLCYTRMVYMPMSYLYGKRFVGPLTPL 300

Query: 301 ILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAF-K 360
           I+ LR+E++ QPY  I WN  R  CAKED  +   L Q L WD L  F EPIL +W   K
Sbjct: 301 IMLLRKELHLQPYEEINWNKARRLCAKEDMIYPHPLVQDLLWDTLHNFVEPILTNWPLKK 360

Query: 361 TIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------ 420
            +R +AL++A  HI YED NSHYITIGC+EKV  +L                        
Sbjct: 361 LVREKALRVAMEHIHYEDENSHYITIGCVEKVLCMLACWIENPNGDHFKKHLARIPDFMW 420

Query: 421 -------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRS 480
                  + SF SQ WD  FAIQA+LA +L  E  + L+KGH FIK+SQVRENPS DF+S
Sbjct: 421 VAEDGLKMQSFGSQLWDTVFAIQALLACDLSDETDDVLRKGHSFIKKSQVRENPSGDFKS 480

Query: 481 MYRHISKGSWTFSDQDHGWQVCDDTAE--------------------------------- 540
           MYRHISKG+WT SD+DHGWQV D TAE                                 
Sbjct: 481 MYRHISKGAWTLSDRDHGWQVSDCTAEALKCCMLLSMMPAEVVGQKIDPEQLYDSVNLLL 540

Query: 541 NLLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPS 600
           +L  + GG++AWEP      W E LNP +FF   + E EYVECT A IQAL+LFK+L+P 
Sbjct: 541 SLQGEKGGLTAWEPV-RAQEWLELLNPTDFFTCVMAEREYVECTSAVIQALVLFKQLYPD 600

Query: 601 HRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQ 660
           HR KEI   + K V+++E  Q  DGSW+GNWGIC IYAT+FA+ GL A+G TY++   ++
Sbjct: 601 HRTKEIIKSIEKGVQFIESKQTPDGSWHGNWGICFIYATWFALSGLAAAGKTYKSCLAVR 660

Query: 661 RGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTP 709
           +GV+FLL IQ  DGGWGESH+SC E++YIPL GN SNLVQT++A++ LI + Q +RDPTP
Sbjct: 661 KGVDFLLAIQEEDGGWGESHLSCPEQRYIPLEGNRSNLVQTAWAMMGLIHAGQAERDPTP 720

BLAST of CmoCh05G009430 vs. TAIR 10
Match: AT1G78950.1 (Terpenoid cyclases family protein )

HSP 1 Score: 859.8 bits (2220), Expect = 1.6e-249
Identity = 426/786 (54.20%), Postives = 523/786 (66.54%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCS 60
           MWRLK+GEG  +DPYLF++NNF GRQTW+F+PD G+PEER  V EAR+ +Y NRF V  S
Sbjct: 1   MWRLKIGEGNGDDPYLFTTNNFAGRQTWEFDPDGGSPEERHSVVEARRIFYDNRFHVKAS 60

Query: 61  SDLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEA-IINKETASIALRRATKFF 120
           SDL W              RMQFLRE+ F+Q I  VKVE++  +  ETA+ ALRR   FF
Sbjct: 61  SDLLW--------------RMQFLREKKFEQRIAPVKVEDSEKVTFETATSALRRGIHFF 120

Query: 121 AALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGW 180
           +ALQ+  GHWPAEN+GP+F+ PPLVF LYITGHL  +F+ EHRKEILRY YCHQ EDGGW
Sbjct: 121 SALQASDGHWPAENAGPLFFLPPLVFCLYITGHLDEVFTSEHRKEILRYIYCHQKEDGGW 180

Query: 181 GLHIEGQSCMLCTVFNYIQLRLLGEEPD---KDECFRARKWILDHGGAIYIPSWGKIWLS 240
           GLHIEG S M CT  NYI +R+LGE PD    + C RAR+WIL HGG  YIPSWGK WLS
Sbjct: 181 GLHIEGHSTMFCTTLNYICMRILGESPDGGHDNACGRAREWILSHGGVTYIPSWGKTWLS 240

Query: 241 VSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPL 300
              ILGV++W G+NPMPPEFW+L    P  P  ++ Y R+  LPMSYL+GKRFVGP+T L
Sbjct: 241 ---ILGVFDWSGSNPMPPEFWILPSFFPVHPAKMWSYCRMVYLPMSYLYGKRFVGPITSL 300

Query: 301 ILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAF-K 360
           ILQLR+E+Y QPY  I W   RH CAKED  + R L Q+L WD+L  F EP L  W F K
Sbjct: 301 ILQLRKELYLQPYEEINWMKVRHLCAKEDTYYPRPLVQELVWDSLYIFAEPFLARWPFNK 360

Query: 361 TIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------ 420
            +R +ALQ+A +HI YED NS YITIGC+EKV  +L                        
Sbjct: 361 LLREKALQLAMKHIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKKHLSRISDYLW 420

Query: 421 -------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRS 480
                  + SF SQ WD  FA+QA+LA+NL  E S+ L++GH+FIK SQV ENPS D++S
Sbjct: 421 MAEDGMKMQSFGSQLWDTGFAMQALLASNLSSEISDVLRRGHEFIKNSQVGENPSGDYKS 480

Query: 481 MYRHISKGSWTFSDQDHGWQVCDDTAE--------------------------------- 540
           MYRHISKG+WTFSD+DHGWQV D TA                                  
Sbjct: 481 MYRHISKGAWTFSDRDHGWQVSDCTAHGLKCCLLFSMLAPDIVGPKQDPERLHDSVNILL 540

Query: 541 NLLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPS 600
           +L +KNGG++AWEP  G P W E LNP E F   ++E EY ECT ++IQAL LFK+L+P 
Sbjct: 541 SLQSKNGGMTAWEP-AGAPKWLELLNPTEMFSDIVIEHEYSECTSSAIQALSLFKQLYPD 600

Query: 601 HRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQ 660
           HR  EI  F+ KA +YLE  Q  DGSWYGNWGIC  Y T+FA+ GL A+G T+ +   ++
Sbjct: 601 HRTTEITAFIKKAAEYLENMQTRDGSWYGNWGICFTYGTWFALAGLAAAGKTFNDCEAIR 660

Query: 661 RGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTP 716
           +GV+FLL  Q  +GGWGES++SC +K YI   G  SN+VQT++AL+ LI S Q +RDP P
Sbjct: 661 KGVQFLLAAQKDNGGWGESYLSCSKKIYIAQVGEISNVVQTAWALMGLIHSGQAERDPIP 720

BLAST of CmoCh05G009430 vs. TAIR 10
Match: AT1G78955.1 (camelliol C synthase 1 )

HSP 1 Score: 855.9 bits (2210), Expect = 2.3e-248
Identity = 420/786 (53.44%), Postives = 527/786 (67.05%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCS 60
           MW+LK+  G   +PYLFS+NNF+GRQTW+F+PD GT EE A VEEAR+ +Y +RF+V  S
Sbjct: 1   MWKLKIANGNKEEPYLFSTNNFLGRQTWEFDPDAGTVEELAAVEEARRKFYDDRFRVKAS 60

Query: 61  SDLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVKVEEA-IINKETASIALRRATKFF 120
           SDL W              RMQFL+E+ F+Q IP  KVE+A  I  E A+ ALR+   F 
Sbjct: 61  SDLIW--------------RMQFLKEKKFEQVIPPAKVEDANNITSEIATNALRKGVNFL 120

Query: 121 AALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGW 180
           +ALQ+  GHWPAEN+GP+F+ PPLVF LY+TGHL  IF+++HR+E+LRY YCHQNEDGGW
Sbjct: 121 SALQASDGHWPAENAGPLFFLPPLVFCLYVTGHLHEIFTQDHRREVLRYIYCHQNEDGGW 180

Query: 181 GLHIEGQSCMLCTVFNYIQLRLLGEEPD---KDECFRARKWILDHGGAIYIPSWGKIWLS 240
           GLHIEG S M CT  NYI +R+LGE P+    + C RAR WILDHGGA YIPSWGK WLS
Sbjct: 181 GLHIEGNSTMFCTTLNYICMRILGEGPNGGPGNACKRARDWILDHGGATYIPSWGKTWLS 240

Query: 241 VSTILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPL 300
              ILGV++W G+NPMPPEFW+L   LP  P  ++CY RL  +PMSYL+GKRFVGP++PL
Sbjct: 241 ---ILGVFDWSGSNPMPPEFWILPSFLPIHPAKMWCYCRLVYMPMSYLYGKRFVGPISPL 300

Query: 301 ILQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAF-K 360
           ILQLR+EIY QPY  I WN  RH CAKED        Q + W+ L  F EP L  W F K
Sbjct: 301 ILQLREEIYLQPYAKINWNRARHLCAKEDAYCPHPQIQDVIWNCLYIFTEPFLACWPFNK 360

Query: 361 TIRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------ 420
            +R +AL +A +HI YED NS YITIGC+EK   +L                        
Sbjct: 361 LLREKALGVAMKHIHYEDENSRYITIGCVEKALCMLACWVEDPNGIHFKKHLLRISDYLW 420

Query: 421 -------LFSF-SQSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRS 480
                  + SF SQ WD  FA+QA++A+NL +E  + L++G+DF+K SQVRENPS DF +
Sbjct: 421 IAEDGMKMQSFGSQLWDSGFALQALVASNLVNEIPDVLRRGYDFLKNSQVRENPSGDFTN 480

Query: 481 MYRHISKGSWTFSDQDHGWQVCDDTAE--------------------------------- 540
           MYRHISKGSWTFSD+DHGWQ  D TAE                                 
Sbjct: 481 MYRHISKGSWTFSDRDHGWQASDCTAESFKCCLLLSMIPPDIVGPKMDPEQLYEAVTILL 540

Query: 541 NLLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPS 600
           +L +KNGGV+AWEP  G   W E LNP E F   ++E EY ECT ++IQALILFK+L+P+
Sbjct: 541 SLQSKNGGVTAWEPARG-QEWLELLNPTEVFADIVVEHEYNECTSSAIQALILFKQLYPN 600

Query: 601 HRKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQ 660
           HR +EI T + KAV+Y+E  Q+ DGSWYG+WG+C  Y+T+F + GL A+G TY N   ++
Sbjct: 601 HRTEEINTSIKKAVQYIESIQMLDGSWYGSWGVCFTYSTWFGLGGLAAAGKTYNNCLAMR 660

Query: 661 RGVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTP 716
           +GV FLL  Q  +GGWGES++SC +K+YIP  G  SNLVQTS+A++ L+ + Q +RDP+P
Sbjct: 661 KGVHFLLTTQKDNGGWGESYLSCPKKRYIPSEGERSNLVQTSWAMMGLLHAGQAERDPSP 720

BLAST of CmoCh05G009430 vs. TAIR 10
Match: AT1G78970.2 (lupeol synthase 1 )

HSP 1 Score: 840.9 bits (2171), Expect = 7.7e-244
Identity = 422/778 (54.24%), Postives = 516/778 (66.32%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCS 60
           MW+LK+G+G   DP+LFSSNNF+GRQTW F+   G+PEERA VEEAR+ +  NRF+V   
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 61  SDLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVK---VEEAIINKETASIALRRATK 120
           SDL W              RMQFLRE+ F+Q IP +K   +EE  I  ET + ALRR  +
Sbjct: 61  SDLLW--------------RMQFLREKKFEQGIPQLKATNIEE--ITYETTTNALRRGVR 120

Query: 121 FFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDG 180
           +F ALQ+  GHWP E +GP+F+ PPL+F LYITGHL  +F  EHRKE+LR+ YCHQNEDG
Sbjct: 121 YFTALQASDGHWPGEITGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQNEDG 180

Query: 181 GWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSV 240
           GWGLHIE +S M CTV NYI LR+LGE P++D C RAR+WILD GG I+IPSWGK WLS 
Sbjct: 181 GWGLHIESKSVMFCTVLNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLS- 240

Query: 241 STILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLI 300
             ILGVY+W G NP PPE  +L   LP  P  + CYSR+  +PMSYL+GKRFVGP+TPLI
Sbjct: 241 --ILGVYDWSGTNPTPPELLMLPSFLPIHPGKILCYSRMVSIPMSYLYGKRFVGPITPLI 300

Query: 301 LQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAF-KT 360
           L LR+E+Y +PY  I W  +R   AKED  +   L Q L  D LQ F EP+L  W   K 
Sbjct: 301 LLLREELYLEPYEEINWKKSRRLYAKEDMYYAHPLVQDLLSDTLQNFVEPLLTRWPLNKL 360

Query: 361 IRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------- 420
           +R +ALQ+  +HI YED NSHYITIGC+EKV  +L                         
Sbjct: 361 VREKALQLTMKHIHYEDENSHYITIGCVEKVLCMLACWVENPNGDYFKKHLARIPDYMWV 420

Query: 421 ------LFSFS-QSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSM 480
                 + SF  Q WD  FAIQA+LA+NL  E  + LK+GH++IK SQVRENPS DFRSM
Sbjct: 421 AEDGMKMQSFGCQLWDTGFAIQALLASNLPDETDDALKRGHNYIKASQVRENPSGDFRSM 480

Query: 481 YRHISKGSWTFSDQDHGWQVCDDTAE---------------------------------N 540
           YRHISKG+WTFSD+DHGWQV D TAE                                 +
Sbjct: 481 YRHISKGAWTFSDRDHGWQVSDCTAEALKCCLLLSMMSADIVGQKIDDEQLYDSVNLLLS 540

Query: 541 LLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSH 600
           L + NGGV+AWEP+     W E LNP EF   T++E E+VECT + IQAL LF+KL+P H
Sbjct: 541 LQSGNGGVNAWEPSRAY-KWLELLNPTEFMANTMVEREFVECTSSVIQALDLFRKLYPDH 600

Query: 601 RKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQR 660
           RKKEI   + KAV+++++ Q  DGSWYGNWG+C IYAT+FA+ GL A+G TY +   ++ 
Sbjct: 601 RKKEINRSIEKAVQFIQDNQTPDGSWYGNWGVCFIYATWFALGGLAAAGETYNDCLAMRN 660

Query: 661 GVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPL 709
           GV FLL  Q  DGGWGES++SC E++YIP  G  SNLVQTS+A++ALI + Q +RD  PL
Sbjct: 661 GVHFLLTTQRDDGGWGESYLSCSEQRYIPSEGERSNLVQTSWAMMALIHTGQAERDLIPL 720

BLAST of CmoCh05G009430 vs. TAIR 10
Match: AT1G78970.1 (lupeol synthase 1 )

HSP 1 Score: 840.9 bits (2171), Expect = 7.7e-244
Identity = 422/778 (54.24%), Postives = 516/778 (66.32%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCS 60
           MW+LK+G+G   DP+LFSSNNF+GRQTW F+   G+PEERA VEEAR+ +  NRF+V   
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 61  SDLFWKFQKWNYNKLLNIMRMQFLRERNFKQTIPIVK---VEEAIINKETASIALRRATK 120
           SDL W              RMQFLRE+ F+Q IP +K   +EE  I  ET + ALRR  +
Sbjct: 61  SDLLW--------------RMQFLREKKFEQGIPQLKATNIEE--ITYETTTNALRRGVR 120

Query: 121 FFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDG 180
           +F ALQ+  GHWP E +GP+F+ PPL+F LYITGHL  +F  EHRKE+LR+ YCHQNEDG
Sbjct: 121 YFTALQASDGHWPGEITGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQNEDG 180

Query: 181 GWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSV 240
           GWGLHIE +S M CTV NYI LR+LGE P++D C RAR+WILD GG I+IPSWGK WLS 
Sbjct: 181 GWGLHIESKSVMFCTVLNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLS- 240

Query: 241 STILGVYEWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLI 300
             ILGVY+W G NP PPE  +L   LP  P  + CYSR+  +PMSYL+GKRFVGP+TPLI
Sbjct: 241 --ILGVYDWSGTNPTPPELLMLPSFLPIHPGKILCYSRMVSIPMSYLYGKRFVGPITPLI 300

Query: 301 LQLRQEIYTQPYNHIKWNPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAF-KT 360
           L LR+E+Y +PY  I W  +R   AKED  +   L Q L  D LQ F EP+L  W   K 
Sbjct: 301 LLLREELYLEPYEEINWKKSRRLYAKEDMYYAHPLVQDLLSDTLQNFVEPLLTRWPLNKL 360

Query: 361 IRNRALQIAKRHIDYEDHNSHYITIGCIEKVNFIL------------------------- 420
           +R +ALQ+  +HI YED NSHYITIGC+EKV  +L                         
Sbjct: 361 VREKALQLTMKHIHYEDENSHYITIGCVEKVLCMLACWVENPNGDYFKKHLARIPDYMWV 420

Query: 421 ------LFSFS-QSWDVAFAIQAILATNLHHEFSETLKKGHDFIKQSQVRENPSSDFRSM 480
                 + SF  Q WD  FAIQA+LA+NL  E  + LK+GH++IK SQVRENPS DFRSM
Sbjct: 421 AEDGMKMQSFGCQLWDTGFAIQALLASNLPDETDDALKRGHNYIKASQVRENPSGDFRSM 480

Query: 481 YRHISKGSWTFSDQDHGWQVCDDTAE---------------------------------N 540
           YRHISKG+WTFSD+DHGWQV D TAE                                 +
Sbjct: 481 YRHISKGAWTFSDRDHGWQVSDCTAEALKCCLLLSMMSADIVGQKIDDEQLYDSVNLLLS 540

Query: 541 LLAKNGGVSAWEPTGGVPSWFERLNPVEFFEYTILELEYVECTLASIQALILFKKLFPSH 600
           L + NGGV+AWEP+     W E LNP EF   T++E E+VECT + IQAL LF+KL+P H
Sbjct: 541 LQSGNGGVNAWEPSRAY-KWLELLNPTEFMANTMVEREFVECTSSVIQALDLFRKLYPDH 600

Query: 601 RKKEIETFLSKAVKYLEETQIEDGSWYGNWGICHIYATYFAIKGLVASGNTYQNSSTLQR 660
           RKKEI   + KAV+++++ Q  DGSWYGNWG+C IYAT+FA+ GL A+G TY +   ++ 
Sbjct: 601 RKKEINRSIEKAVQFIQDNQTPDGSWYGNWGVCFIYATWFALGGLAAAGETYNDCLAMRN 660

Query: 661 GVEFLLKIQCPDGGWGESHISCREKKYIPLPGNSSNLVQTSFALIALILSQQEKRDPTPL 709
           GV FLL  Q  DGGWGES++SC E++YIP  G  SNLVQTS+A++ALI + Q +RD  PL
Sbjct: 661 GVHFLLTTQRDDGGWGESYLSCSEQRYIPSEGERSNLVQTSWAMMALIHTGQAERDLIPL 720

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O821402.2e-25955.73Beta-amyrin synthase 1 OS=Panax ginseng OX=4054 GN=OSCPNY1 PE=1 SV=1[more]
Q8W3Z11.8e-25854.65Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1[more]
E7DN631.8e-25855.61Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1[more]
A8CDT23.1e-25855.16Beta-amyrin synthase OS=Bruguiera gymnorhiza OX=39984 GN=BAS PE=1 SV=1[more]
O821465.3e-25855.48Beta-amyrin synthase 2 OS=Panax ginseng OX=4054 GN=OSCPNY2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G3T60.0e+0079.19Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505... [more]
A0A6J1G4X90.0e+0079.10Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507... [more]
A0A6J1G3X40.0e+0078.43Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505... [more]
A0A6J1G4T20.0e+0078.40Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507... [more]
A0A6J1KCM30.0e+0078.53Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493703... [more]
Match NameE-valueIdentityDescription
XP_022946462.10.0e+0079.19beta-amyrin synthase-like isoform X3 [Cucurbita moschata][more]
XP_022946794.10.0e+0079.10LOW QUALITY PROTEIN: beta-amyrin synthase-like [Cucurbita moschata][more]
XP_023545920.10.0e+0079.62LOW QUALITY PROTEIN: beta-Amyrin Synthase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022946460.10.0e+0078.43beta-amyrin synthase-like isoform X1 [Cucurbita moschata] >XP_022946461.1 beta-a... [more]
XP_022946788.10.0e+0078.40beta-amyrin synthase-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G78960.12.5e-25055.33lupeol synthase 2 [more]
AT1G78950.11.6e-24954.20Terpenoid cyclases family protein [more]
AT1G78955.12.3e-24853.44camelliol C synthase 1 [more]
AT1G78970.27.7e-24454.24lupeol synthase 1 [more]
AT1G78970.17.7e-24454.24lupeol synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032696Squalene cyclase, C-terminalPFAMPF13243SQHop_cyclase_Ccoord: 529..709
e-value: 1.4E-32
score: 113.0
NoneNo IPR availableGENE3D1.50.10.20coord: 384..714
e-value: 1.3E-104
score: 351.9
NoneNo IPR availableGENE3D1.50.10.20coord: 79..230
e-value: 1.4E-9
score: 39.5
NoneNo IPR availablePANTHERPTHR11764:SF58LUPEOL SYNTHASE 1coord: 1..390
coord: 395..471
NoneNo IPR availablePANTHERPTHR11764:SF58LUPEOL SYNTHASE 1coord: 472..713
IPR032697Squalene cyclase, N-terminalPFAMPF13249SQHop_cyclase_Ncoord: 112..369
e-value: 1.5E-35
score: 122.7
IPR018333Squalene cyclasePANTHERPTHR11764TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 472..713
IPR018333Squalene cyclasePANTHERPTHR11764TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 1..390
coord: 395..471
IPR018333Squalene cyclaseCDDcd02892SQCY_1coord: 111..708
e-value: 0.0
score: 724.757
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 364..712
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 134..390

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G009430.1CmoCh05G009430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016104 triterpenoid biosynthetic process
cellular_component GO:0005811 lipid droplet
molecular_function GO:0042300 beta-amyrin synthase activity
molecular_function GO:0000250 lanosterol synthase activity
molecular_function GO:0016866 intramolecular transferase activity