CmoCh05G008530 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G008530
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionexopolygalacturonase-like
LocationCmo_Chr05: 5436446 .. 5437796 (+)
RNA-Seq ExpressionCmoCh05G008530
SyntenyCmoCh05G008530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTAATACCACTGTTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACGACATTTGGTGCCAAACCTAATAATGATGTTACTCTAAGTTGATAAACCAAAATTTCATTTTGTACAAAATATTTTTCTCAAATGAAATGGTTGAATTCACCGGCTTTAGCAGATGCATGGAAGGAAGCATGTATTTCGGCTACTCCAAGTAAAGTATTGGTTCTAAAAGGAACTTATCAATTAGGAGCATCCACACTCAAAGGTCCATGTAAAGGTCCTATTGAGCTTCTAGTTGAAGGAACATTACAAGCTTCTCCTACCGGAAGGAGATGGGTTGCTTGTTATGGAATACTTCGATCATTTGACATTGTCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTTGTTGGGAAAAGAATGATTGCCACATGAAGAAAGAATGCAAAAAGCTTCCCATGGTAAATGCTCGTACTACTTTAATTATAAAAAGAACTCGTAATTTGGGTTAGCATTATATGCTTTAACATGGGAGTGTTTTTTCTTTGTTTGTTTAAAATGGGCAGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGACGGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGACAATAGTCCCAACACCGATGGAATTTACTTGGGCGATTCAGAAGACGTTTACATTCTCAATACCGTAATCGCAACGGGAGATGATTGTATATCTGTAGGATACACTAACAGAAAAATAACCATTAGTGATGTTACTTGCGGACAGGGACATGACATTAGCATAGGCAGGTTGGGCAAGTATAAAACTGAAAAAGAACTGGTAGGAGTTACCGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGTCAGATTCGGCCGTCTCATACCCAACCTCTAACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTGTGTATTAAAATTGAACCTCCTTGGTTTCCAACTTATTATCTTTTGAACAAGTATTAATTAATGTTTACTTTGAACATCCAGGTTCCATCAGCAATTAAGATTAGCAAAGTTAGTTTCAAGAACTTCAGGGGCACATCTTCAACTCGGTTGCAGTCAAGCTTGTTTGCAGTAAAAGTAATCCATGTGAGGATGTGGAATTTCCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGTCAATTGTGA

mRNA sequence

ATGGCATTAATACCACTGTTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACGACATTTGGTGCCAAACCTAATAATGATGAACATTACAAGCTTCTCCTACCGGAAGGAGATGGGTTGCTTGTTATGGAATACTTCGATCATTTGACATTGTCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTTGTTGGGAAAAGAATGATTGCCACATGAAGAAAGAATGCAAAAAGCTTCCCATGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGACGGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGACAATAGTCCCAACACCGATGGAATTTACTTGGGCGATTCAGAAGACGTTTACATTCTCAATACCGTAATCGCAACGGGAGATGATTGTATATCTGTAGGATACACTAACAGAAAAATAACCATTAGTGATGTTACTTGCGGACAGGGACATGACATTAGCATAGGCAGGTTGGGCAAGTATAAAACTGAAAAAGAACTGGTAGGAGTTACCGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGTCAGATTCGGCCGTCTCATACCCAACCTCTAACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTTCCATCAGCAATTAAGATTAGCAAAGTTAGTTTCAAGAACTTCAGGGGCACATCTTCAACTCGGTTGCAGTCAAGCTTGTTTGCAGTAAAAGTAATCCATGTGAGGATGTGGAATTTCCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGTCAATTGTGA

Coding sequence (CDS)

ATGGCATTAATACCACTGTTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACGACATTTGGTGCCAAACCTAATAATGATGAACATTACAAGCTTCTCCTACCGGAAGGAGATGGGTTGCTTGTTATGGAATACTTCGATCATTTGACATTGTCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTTGTTGGGAAAAGAATGATTGCCACATGAAGAAAGAATGCAAAAAGCTTCCCATGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGACGGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGACAATAGTCCCAACACCGATGGAATTTACTTGGGCGATTCAGAAGACGTTTACATTCTCAATACCGTAATCGCAACGGGAGATGATTGTATATCTGTAGGATACACTAACAGAAAAATAACCATTAGTGATGTTACTTGCGGACAGGGACATGACATTAGCATAGGCAGGTTGGGCAAGTATAAAACTGAAAAAGAACTGGTAGGAGTTACCGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGTCAGATTCGGCCGTCTCATACCCAACCTCTAACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTTCCATCAGCAATTAAGATTAGCAAAGTTAGTTTCAAGAACTTCAGGGGCACATCTTCAACTCGGTTGCAGTCAAGCTTGTTTGCAGTAAAAGTAATCCATGTGAGGATGTGGAATTTCCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGTCAATTGTGA

Protein sequence

MALIPLLLLLVSNATADATSGVFDITTFGAKPNNDEHYKLLLPEGDGLLVMEYFDHLTLSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSSTRLQSSLFAVKVIHVRMWNFLILTSLIVVKKGQL
Homology
BLAST of CmoCh05G008530 vs. ExPASy Swiss-Prot
Match: P35337 (Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-77
Identity = 130/239 (54.39%), Postives = 177/239 (74.06%), Query Frame = 0

Query: 46  DGLLVMEYFDHLTLSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDIT 105
           D  +V E  +   L+G G FDG+G A W+ N+CH   ECKKLP++++F+F+ N+ +KD+T
Sbjct: 96  DRWVVFEKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVRFDFVDNAEIKDVT 155

Query: 106 SLDSKNFHINVLTGKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCISV 165
           SLD+KNFH NV++GKN+TFD++KI AP  SPNTDGI+LG  E V ILNT IATGDDCISV
Sbjct: 156 SLDAKNFHFNVISGKNMTFDNIKIIAPAESPNTDGIHLGRCEGVKILNTKIATGDDCISV 215

Query: 166 GYTNRKITISDVTCGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSA 225
           G   + + I  V CG GH IS+G LG+Y  E+++  +TVK C L GT+NG+RIK+W  +A
Sbjct: 216 GDGMKNLLIEKVVCGPGHGISVGSLGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAA 275

Query: 226 VSYPTSNMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSSTR 285
            +   + +HFEDI ++ V+NPI+IDQEYCPWNQCN+  PS IK+  ++F+N RGTS  +
Sbjct: 276 CTTTAAGIHFEDIILNKVSNPILIDQEYCPWNQCNKNKPSTIKLVDITFRNIRGTSENK 334

BLAST of CmoCh05G008530 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.9e-74
Identity = 151/323 (46.75%), Postives = 192/323 (59.44%), Query Frame = 0

Query: 18  ATSGVFDITTFGAKPNND----------------EHYKLLLPEG---------------- 77
           A SGV DI+ FG KPN+D                   K+++P G                
Sbjct: 20  AQSGVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAP 79

Query: 78  -----DGLLVME----------------YFDHLTLSGTGVFDGQGKACWEK----NDCHM 137
                DG +                   Y DHLTLSG GVFDGQG   ++K    +    
Sbjct: 80  IELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSG 139

Query: 138 KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKITAPDNSPNTDG 197
           K    K+ MN  FNF+ NSIV+ +TS DSKNFH+ V   KN+TFD   ITAP +SPNTDG
Sbjct: 140 KNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDG 199

Query: 198 IYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRLGKYKTEKELV 257
           I++G S DV ILNT I TGDDC+S+G  +++IT+  V CG GH +S+G LGK+ TE+ + 
Sbjct: 200 IHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVE 259

Query: 258 GVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIIDQEYCPWNQCN 284
           G+TVK C L  T NGVRIK+W D+  +   S++HFEDI M NV NP+IIDQEY PWNQC+
Sbjct: 260 GITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCS 319

BLAST of CmoCh05G008530 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.3e-74
Identity = 131/242 (54.13%), Postives = 179/242 (73.97%), Query Frame = 0

Query: 44  EGDGLLVMEYFDHLTLSGTGVFDGQGKACWEKNDCHMKKE-CKKLPMNLKFNFITNSIVK 103
           +G G + +   D LT+ G GVFDGQGK+ W +NDCH     CK L MNL+   +TNSI++
Sbjct: 62  KGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILR 121

Query: 104 DITSLDSKNFHINVLTGKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDC 163
           D+T+LDSKNFH+NV+  KNLTF+  KI+A + S NTDGI++G S+ V I+NT I TGDDC
Sbjct: 122 DVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDC 181

Query: 164 ISVGYTNRKITISDVTCGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWS 223
           IS+G  ++ I I+++TCG GH IS+G LG+YK E+ +VG+ VK C + G+ NGVRIK+W 
Sbjct: 182 ISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWP 241

Query: 224 DSAVSYPTSNMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSS 283
            S      S MHF+DI M++V  PI+IDQ YCP+NQC  +VPS++K+SK+SFKN +GTS+
Sbjct: 242 KSEPG-EASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTST 301

Query: 284 TR 285
           T+
Sbjct: 302 TK 302

BLAST of CmoCh05G008530 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-71
Identity = 126/242 (52.07%), Postives = 174/242 (71.90%), Query Frame = 0

Query: 44  EGDGLLVMEYFDHLTLSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKD 103
           + DG +     D LT+SGTG  DGQG+  W KN+C     CK   MNL+F+F+ +++V+D
Sbjct: 84  KSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNLRFDFLKHAMVRD 143

Query: 104 ITSLDSKNFHINVLTGKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCI 163
           ITSL+SK FHINVL  +++TF H+ +TAP  S NTDGI++G S+ V I NT IATGDDCI
Sbjct: 144 ITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCI 203

Query: 164 SVGYTNRKITISDVTCGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSD 223
           S+G  ++ +TI+ V CG GH ISIG LG+Y  EKE+ G+TVK C   GT NGVR+K+W +
Sbjct: 204 SIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPN 263

Query: 224 SAVSYPTSNMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSST 283
           S     T ++ F+D+ M+NV NP+I+DQEYCP+ QC+R+ PS IK+S ++F N RGTS+ 
Sbjct: 264 SPPGAAT-DLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTG 323

Query: 284 RL 286
           ++
Sbjct: 324 KV 324

BLAST of CmoCh05G008530 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.9e-67
Identity = 115/228 (50.44%), Postives = 163/228 (71.49%), Query Frame = 0

Query: 55  DHLTLSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHI 114
           ++  + G G+FDGQG   +EKN C  ++   KLP+N++F+F+TN++++DITS DSK FHI
Sbjct: 116 ENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHI 175

Query: 115 NVLTGKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITI 174
           NV   KN+T + LKI APD SPNTDGI++G SE V I+ + I TGDDCIS+G   + + I
Sbjct: 176 NVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVI 235

Query: 175 SDVTCGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMH 234
            ++TCG GH ISIG LGK++ E+ + G+ +  C +  T+NG RIK+W         S +H
Sbjct: 236 KEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEH-GGAVSEIH 295

Query: 235 FEDIEMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSS 283
           FEDI M+NV++PI+IDQ+YCPWN+C +   S +K+S +SFKN RGTS+
Sbjct: 296 FEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSA 342

BLAST of CmoCh05G008530 vs. ExPASy TrEMBL
Match: A0A6J1I9Y3 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472545 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.7e-139
Identity = 262/343 (76.38%), Postives = 271/343 (79.01%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNND----------------EHYKLLLP- 60
           MALIPLLLLLVSNAT DATSGVFDITTFGAKPN D                   K+L+P 
Sbjct: 8   MALIPLLLLLVSNATVDATSGVFDITTFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTLSGTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLSGTGVFDGQGK 127

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP++SPNTDGI+LGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCG GH ISIG L
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

BLAST of CmoCh05G008530 vs. ExPASy TrEMBL
Match: A0A6J1IE44 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111474737 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.7e-139
Identity = 262/343 (76.38%), Postives = 271/343 (79.01%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNND----------------EHYKLLLP- 60
           MALIPLLLLLVSNAT DATSGVFDITTFGAKPN D                   K+L+P 
Sbjct: 8   MALIPLLLLLVSNATVDATSGVFDITTFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTLSGTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLSGTGVFDGQGK 127

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP++SPNTDGI+LGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCG GH ISIG L
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

BLAST of CmoCh05G008530 vs. ExPASy TrEMBL
Match: A0A6J1G454 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111450616 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.5e-138
Identity = 260/343 (75.80%), Postives = 270/343 (78.72%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNNDEHY----------------KLLLP- 60
           MAL+PLLLLLVSNAT DATSGVFDITTFGAKPN D                   K+L+P 
Sbjct: 8   MALMPLLLLLVSNATVDATSGVFDITTFGAKPNADATQALVDAWKEACASATPSKVLVPK 67

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTL+GTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP NSPNTDGI+LGDSEDVYILNT IATGDDC+SVGYTNRKITISDVTCG GH ISIG L
Sbjct: 188 APHNSPNTDGIHLGDSEDVYILNTAIATGDDCVSVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

BLAST of CmoCh05G008530 vs. ExPASy TrEMBL
Match: E3VSV7 (Polygalacturonase OS=Cucurbita pepo OX=3663 GN=PG1 PE=2 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.7e-138
Identity = 261/343 (76.09%), Postives = 269/343 (78.43%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNNDEHY----------------KLLLP- 60
           MALIPLLLLLVSNAT  ATSGVFDITTFGAKPN D                   K+L+P 
Sbjct: 18  MALIPLLLLLVSNATVGATSGVFDITTFGAKPNADATQALADAWKEACASATPSKVLVPK 77

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTLSGTGVFDGQGK
Sbjct: 78  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYIDHLTLSGTGVFDGQGK 137

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 138 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 197

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP NSPNTDGI+LGDSEDVYILNT IATGDDCISVGYTNRKITISDVTCG GH ISIG L
Sbjct: 198 APHNSPNTDGIHLGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 257

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEM+NVANPIIID
Sbjct: 258 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIIID 317

BLAST of CmoCh05G008530 vs. ExPASy TrEMBL
Match: A0A6J1ENZ5 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111436229 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 9.7e-138
Identity = 260/343 (75.80%), Postives = 270/343 (78.72%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNND----------------EHYKLLLP- 60
           MALIPLLLLLVSNATADATSGVFDIT FGAKPN D                   K+L+P 
Sbjct: 8   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTL+GTGVFDGQGK
Sbjct: 68  GTYQLGASRLKGPCKSPIELLVEGTLQASPSDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP NSPNTDGI++GDSEDVYILNT IATGDDCISVGYTNRKITISDVTCG GH ISIG L
Sbjct: 188 APHNSPNTDGIHMGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEM+NVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIIID 307

BLAST of CmoCh05G008530 vs. NCBI nr
Match: XP_022972848.1 (exopolygalacturonase-like [Cucurbita maxima] >XP_022973915.1 exopolygalacturonase-like [Cucurbita maxima] >XP_022973916.1 exopolygalacturonase-like [Cucurbita maxima] >XP_022973932.1 exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 503.1 bits (1294), Expect = 1.8e-138
Identity = 262/343 (76.38%), Postives = 271/343 (79.01%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNND----------------EHYKLLLP- 60
           MALIPLLLLLVSNAT DATSGVFDITTFGAKPN D                   K+L+P 
Sbjct: 8   MALIPLLLLLVSNATVDATSGVFDITTFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTLSGTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLSGTGVFDGQGK 127

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP++SPNTDGI+LGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCG GH ISIG L
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

BLAST of CmoCh05G008530 vs. NCBI nr
Match: XP_022975421.1 (exopolygalacturonase-like, partial [Cucurbita maxima])

HSP 1 Score: 503.1 bits (1294), Expect = 1.8e-138
Identity = 262/343 (76.38%), Postives = 271/343 (79.01%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNND----------------EHYKLLLP- 60
           MALIPLLLLLVSNAT DATSGVFDITTFGAKPN D                   K+L+P 
Sbjct: 8   MALIPLLLLLVSNATVDATSGVFDITTFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTLSGTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLSGTGVFDGQGK 127

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP++SPNTDGI+LGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCG GH ISIG L
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

BLAST of CmoCh05G008530 vs. NCBI nr
Match: XP_022946596.1 (exopolygalacturonase-like [Cucurbita moschata] >XP_022946597.1 exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 501.5 bits (1290), Expect = 5.2e-138
Identity = 260/343 (75.80%), Postives = 270/343 (78.72%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNNDEHY----------------KLLLP- 60
           MAL+PLLLLLVSNAT DATSGVFDITTFGAKPN D                   K+L+P 
Sbjct: 8   MALMPLLLLLVSNATVDATSGVFDITTFGAKPNADATQALVDAWKEACASATPSKVLVPK 67

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTL+GTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP NSPNTDGI+LGDSEDVYILNT IATGDDC+SVGYTNRKITISDVTCG GH ISIG L
Sbjct: 188 APHNSPNTDGIHLGDSEDVYILNTAIATGDDCVSVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

BLAST of CmoCh05G008530 vs. NCBI nr
Match: ADP09681.1 (polygalacturonase [Cucurbita pepo])

HSP 1 Score: 500.4 bits (1287), Expect = 1.2e-137
Identity = 261/343 (76.09%), Postives = 269/343 (78.43%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNNDEHY----------------KLLLP- 60
           MALIPLLLLLVSNAT  ATSGVFDITTFGAKPN D                   K+L+P 
Sbjct: 18  MALIPLLLLLVSNATVGATSGVFDITTFGAKPNADATQALADAWKEACASATPSKVLVPK 77

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTLSGTGVFDGQGK
Sbjct: 78  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYIDHLTLSGTGVFDGQGK 137

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 138 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 197

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP NSPNTDGI+LGDSEDVYILNT IATGDDCISVGYTNRKITISDVTCG GH ISIG L
Sbjct: 198 APHNSPNTDGIHLGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 257

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEM+NVANPIIID
Sbjct: 258 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIIID 317

BLAST of CmoCh05G008530 vs. NCBI nr
Match: XP_022929731.1 (exopolygalacturonase-like [Cucurbita moschata] >XP_022929733.1 exopolygalacturonase-like [Cucurbita moschata] >XP_022929734.1 exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 499.6 bits (1285), Expect = 2.0e-137
Identity = 260/343 (75.80%), Postives = 270/343 (78.72%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITTFGAKPNND----------------EHYKLLLP- 60
           MALIPLLLLLVSNATADATSGVFDIT FGAKPN D                   K+L+P 
Sbjct: 8   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  ---------------------------------EGDGLLVMEYFDHLTLSGTGVFDGQGK 120
                                            EGDGLLVMEY DHLTL+GTGVFDGQGK
Sbjct: 68  GTYQLGASRLKGPCKSPIELLVEGTLQASPSDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 ACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLTGKNLTFDHLKIT 180
           A WEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVL GKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGQGHDISIGRL 240
           AP NSPNTDGI++GDSEDVYILNT IATGDDCISVGYTNRKITISDVTCG GH ISIG L
Sbjct: 188 APHNSPNTDGIHMGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDIEMDNVANPIIID 294
           GKYKTEKE+VGVTVKKCKLIGTTNGVRIKSW DSAVSYP S+MHFEDIEM+NVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIIID 307

BLAST of CmoCh05G008530 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.5e-77
Identity = 127/226 (56.19%), Postives = 174/226 (76.99%), Query Frame = 0

Query: 59  LSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLT 118
           L+G G+FDG+G A W  N+CH   ECKKLP++++F+F+ N+ ++DI+S+D+KNFHINVL 
Sbjct: 110 LNGGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLG 169

Query: 119 GKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVT 178
            KN+TFD++K+ AP  SPNTDGI+LG SE V ILN+ IATGDDCISVG   + + + +V 
Sbjct: 170 AKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVM 229

Query: 179 CGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDI 238
           CG GH IS+G LG+Y  E+++ G+TV  C L GT NG+RIK+W  +A +   S +HFE+I
Sbjct: 230 CGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENI 289

Query: 239 EMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSSTR 285
            ++NV+NPI+IDQEYCPWNQCN++ PS IK+  +SFKN RGTS  +
Sbjct: 290 ILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNK 335

BLAST of CmoCh05G008530 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 3.9e-75
Identity = 127/236 (53.81%), Postives = 174/236 (73.73%), Query Frame = 0

Query: 49  LVMEYFDHLTLSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLD 108
           +V    D   L+G G FDG+G A W  N+CH   ECKKLP++++F+FI NS ++DI+S+D
Sbjct: 99  VVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDISSID 158

Query: 109 SKNFHINVLTGKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYT 168
           +KNFHINVL  KN+T +++KI AP++SPNTDGI+LG S+ V ILN+ I+TGDDCISVG  
Sbjct: 159 AKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGDG 218

Query: 169 NRKITISDVTCGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSY 228
            + + +  VTCG GH IS+G LG+Y  E+++ G+ V  C L  T NG+RIK+W  +A S 
Sbjct: 219 MKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACST 278

Query: 229 PTSNMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSSTR 285
             S++HFEDI + +V+NPI+IDQEYCPWNQCN++  S IK+  +SFKN RGTS  +
Sbjct: 279 TASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNK 334

BLAST of CmoCh05G008530 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 4.4e-74
Identity = 123/236 (52.12%), Postives = 174/236 (73.73%), Query Frame = 0

Query: 49  LVMEYFDHLTLSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLD 108
           +V +  D   L+G G FDG+G A W  N+CH   ECKKLP++++F+F+TN+ ++DI+S+D
Sbjct: 100 VVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVTNAEIRDISSID 159

Query: 109 SKNFHINVLTGKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYT 168
           +KNFHINV+  KN+TFD++KI AP  SPNTDGI+LG S  V I+N+ I+TGDDC+SVG  
Sbjct: 160 AKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSVGDG 219

Query: 169 NRKITISDVTCGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSY 228
              + + +V CG GH IS+G LG+Y  E+++ G+ V  C L  T NG+RIK+W  +A S 
Sbjct: 220 MVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAACST 279

Query: 229 PTSNMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSSTR 285
             SN+HFE+I + NV+NPI+IDQEYCPWNQCN++  S+IK++ +SF+  RGTS  +
Sbjct: 280 TASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSGNK 335

BLAST of CmoCh05G008530 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.1e-72
Identity = 118/226 (52.21%), Postives = 169/226 (74.78%), Query Frame = 0

Query: 59  LSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLT 118
           L+G GVFDG+G A W  N+CH    CKKLP++++F+F+T++ ++ ITSLD+K+FHINV+ 
Sbjct: 109 LNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTDAKIRGITSLDAKHFHINVIG 168

Query: 119 GKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVT 178
            KN+TF+ +KI AP  SPNTDGI++G S+ + I+N+ I+TGDDC+SVG   + + +  VT
Sbjct: 169 AKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDDCVSVGDGMKNLLVERVT 228

Query: 179 CGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDSAVSYPTSNMHFEDI 238
           CG GH ISIG LG+Y  E+ + G+ +  C L  T NG+RIK+W  +A +   S++HFE+I
Sbjct: 229 CGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTWPSAACTTTASDIHFENI 288

Query: 239 EMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSSTR 285
            + NV+NPI+IDQEYCPWNQCN++ PS IK++ +SFK  RGTS  +
Sbjct: 289 LLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTSGNK 334

BLAST of CmoCh05G008530 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 3.7e-65
Identity = 116/239 (48.54%), Postives = 164/239 (68.62%), Query Frame = 0

Query: 45  GDGLLVMEYFDHLTLSGTGVFDGQGKACWEKNDCHMKKECKKLPMNLKFNFITNSIVKDI 104
           G G +   + + LTL+G G FDGQG   W  N+C     CK LP +LKF  +  ++V+ I
Sbjct: 111 GGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNCTSDSNCKLLPTSLKFVGMNRTVVRRI 170

Query: 105 TSLDSKNFHINVLTGKNLTFDHLKITAPDNSPNTDGIYLGDSEDVYILNTVIATGDDCIS 164
           +S++SK FHI ++  ++     L ITAP +SPNTDGI++  S +VY   + IATGDDC+S
Sbjct: 171 SSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIERSSNVYFSRSHIATGDDCVS 230

Query: 165 VGYTNRKITISDVTCGQGHDISIGRLGKYKTEKELVGVTVKKCKLIGTTNGVRIKSWSDS 224
           +G  N +ITI+ + CG GH IS+G LG+Y  EK++ G+ VK CK+ GTTNG+RIK+W++S
Sbjct: 231 IGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVVKDCKISGTTNGIRIKTWANS 290

Query: 225 AVSYPTSNMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNFRGTSST 284
                 +NM FE+I M+NV NPIIIDQ YCP++ C   VPS +++S++ FKN RGTSS+
Sbjct: 291 PGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVPSKVELSEIYFKNIRGTSSS 349

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P353371.4e-7754.39Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1[more]
Q403121.9e-7446.75Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
P245484.3e-7454.13Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q6H9K01.5e-7152.07Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Q397663.9e-6750.44Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I9Y38.7e-13976.38exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472545 PE=3 SV=1[more]
A0A6J1IE448.7e-13976.38exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111474737 PE=3 SV=1[more]
A0A6J1G4542.5e-13875.80exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111450616 PE=3 SV=... [more]
E3VSV75.7e-13876.09Polygalacturonase OS=Cucurbita pepo OX=3663 GN=PG1 PE=2 SV=1[more]
A0A6J1ENZ59.7e-13875.80exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111436229 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_022972848.11.8e-13876.38exopolygalacturonase-like [Cucurbita maxima] >XP_022973915.1 exopolygalacturonas... [more]
XP_022975421.11.8e-13876.38exopolygalacturonase-like, partial [Cucurbita maxima][more]
XP_022946596.15.2e-13875.80exopolygalacturonase-like [Cucurbita moschata] >XP_022946597.1 exopolygalacturon... [more]
ADP09681.11.2e-13776.09polygalacturonase [Cucurbita pepo][more]
XP_022929731.12.0e-13775.80exopolygalacturonase-like [Cucurbita moschata] >XP_022929733.1 exopolygalacturon... [more]
Match NameE-valueIdentityDescription
AT3G07840.12.5e-7756.19Pectin lyase-like superfamily protein [more]
AT3G07820.13.9e-7553.81Pectin lyase-like superfamily protein [more]
AT3G07830.14.4e-7452.12Pectin lyase-like superfamily protein [more]
AT5G48140.14.1e-7252.21Pectin lyase-like superfamily protein [more]
AT4G18180.13.7e-6548.54Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 146..167
e-value: 860.0
score: 7.7
coord: 199..220
e-value: 110.0
score: 11.7
coord: 230..251
e-value: 4900.0
score: 2.3
coord: 119..145
e-value: 250.0
score: 10.5
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 48..286
e-value: 3.1E-76
score: 256.5
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 11..304
e-value: 3.6E-74
score: 251.6
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 50..286
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 50..286
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 11..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G008530.1CmoCh05G008530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity