CmoCh05G008250 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G008250
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionexopolygalacturonase-like
LocationCmo_Chr05: 5020921 .. 5022309 (+)
RNA-Seq ExpressionCmoCh05G008250
SyntenyCmoCh05G008250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGATATCAAAGTTTATGGCATTAATACCACTACTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACAAAATTTGGTGCCAAACCTAATGCTGATGTTACTCAAGTACGTGTACATTTGTTTAAGTTGATAAACCAAAATTTCATTTTGTACAAAATATTTCTCTCAAATGAAATGGTTGAATTCACAGGCTTTAGCAGATGCATGGAAGGAAGCATGTGCGTCGGCTACTCCAAGTAAAGTATTAGTTCCAAAAGGAACTTATCAATTAGGAGAATCCAGGCTCAAAGGTCCATGTAAAAGTCCTATTGAGCTTCTAGTTGAAGGAACATTACAAGCTTCTCCTACCGACACGGAAGGAGATGGGTTGCTTGTTATGGAATACGTCGATCATTTGACATTGGCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTGGTTGGGAAAAGAATGATTGCCACGTGAAGAAAGAATGCAAAAAGCTTCCCATGGTAAATGCTCATACTACTTTAATTATAAAAAGGACCCATACTTTGGGTTAGCATTGTATGTTTTAACACGGAGTGGTTTTTTTTTGTTTGTTTAAAATGAGCAGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGGCCGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGAGCATAGTCCCAACACCGATGGAATTCACTTGGGTGATTCAGAAGACGTTTACATTCTCAATACTGTAATCGCAACGGGAGATGATTGTATATCTGTAGGATACACTAACAGAAAAATAACCATAGTGATGTCACTTGCGGACCGGGACATGGCATTAGCATAGGCAGCTTGGGCAAGTATAAAACTGAAAAAGAAGTGGTAGGAGTTACAGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGCCAGATTCTGTCGTCTCATACCCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTATGTATTAAAATCGTCGAACCTCGTTGGTTTCCAACTTATTATCTTTTGAACAAGTATTAATTCATGTTTGTTTTGAACATCCAGGTTCCATCAACAATTAAGATTAGCAAAGTTAGTTTCAAGAATATCAGGGGCACATCTTCTACTCCCGTTGCAGTCAAACTTGTTTGCAGTAAAAGTCATCCATGTGAGGATGTGGAAATTGCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGTCAATT

mRNA sequence

ATTGATATCAAAGTTTATGGCATTAATACCACTACTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACAAAATTTGGTGCCAAACCTAATGCTGATGTTACTCAAGCTTTAGCAGATGCATGGAAGGAAGCATGTGCGTCGGCTACTCCAAGTAAAGTATTAGTTCCAAAAGGAACTTATCAATTAGGAGAATCCAGGCTCAAAGGTCCATGTAAAAGTCCTATTGAGCTTCTAGTTGAAGGAACATTACAAGCTTCTCCTACCGACACGGAAGGAGATGGGTTGCTTGTTATGGAATACGTCGATCATTTGACATTGGCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTGGTTGGGAAAAGAATGATTGCCACGTGAAGAAAGAATGCAAAAAGCTTCCCATGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGGCCGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGAGCATAGTCCCAACACCGATGGAATTCACTTGGGTGATTCAGAAGACGTTTACATTCTCAATACTGTAATCGCAACGGGAGATGATTGTATATCTAAAAATAACCATAGTGATGTCACTTGCGGACCGGGACATGGCATTAGCATAGGCAGCTTGGGCAAGTATAAAACTGAAAAAGAAGTGGTAGGAGTTACAGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGCCAGATTCTGTCGTCTCATACCCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTTCCATCAACAATTAAGATTAGCAAAGTTAGTTTCAAGAATATCAGGGGCACATCTTCTACTCCCGTTGCAGTCAAACTTGTTTGCAGTAAAAGTCATCCATGTGAGGATGTGGAAATTGCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGTCAATT

Coding sequence (CDS)

ATGGCATTAATACCACTACTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACAAAATTTGGTGCCAAACCTAATGCTGATGTTACTCAAGCTTTAGCAGATGCATGGAAGGAAGCATGTGCGTCGGCTACTCCAAGTAAAGTATTAGTTCCAAAAGGAACTTATCAATTAGGAGAATCCAGGCTCAAAGGTCCATGTAAAAGTCCTATTGAGCTTCTAGTTGAAGGAACATTACAAGCTTCTCCTACCGACACGGAAGGAGATGGGTTGCTTGTTATGGAATACGTCGATCATTTGACATTGGCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTGGTTGGGAAAAGAATGATTGCCACGTGAAGAAAGAATGCAAAAAGCTTCCCATGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGGCCGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGAGCATAGTCCCAACACCGATGGAATTCACTTGGGTGATTCAGAAGACGTTTACATTCTCAATACTGTAATCGCAACGGGAGATGATTGTATATCTAAAAATAACCATAGTGATGTCACTTGCGGACCGGGACATGGCATTAGCATAGGCAGCTTGGGCAAGTATAAAACTGAAAAAGAAGTGGTAGGAGTTACAGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGCCAGATTCTGTCGTCTCATACCCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTTCCATCAACAATTAAGATTAGCAAAGTTAGTTTCAAGAATATCAGGGGCACATCTTCTACTCCCGTTGCAGTCAAACTTGTTTGCAGTAAAAGTCATCCATGTGAGGATGTGGAAATTGCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGTCAATT

Protein sequence

MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSEDVYILNTVIATGDDCISKNNHSDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEGSI
Homology
BLAST of CmoCh05G008250 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 5.5e-110
Identity = 199/333 (59.76%), Postives = 252/333 (75.68%), Query Frame = 0

Query: 35  DVTQALADAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSPIELLVEGTLQ--ASPTD 94
           D TQAL  AWKEACASA+PS +LVPKG + +G   L+GPCKS I L ++GTL+  A P+ 
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 95  TEGDGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKE-CKKLPMNLKFNFITNSIV 154
            +G G + +  +D LT+ G GVFDGQGK+ W +NDCH     CK L MNL+   +TNSI+
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 155 KDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSEDVYILNTVIATGDD 214
           +D+T+LDSKNFH+NV+  KNLTF+  KI+A E S NTDGIH+G S+ V I+NT I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 215 CI-----SKN-NHSDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSW 274
           CI     SKN N +++TCGPGHGIS+GSLG+YK E+ VVG+ VK C + G+ NGVRIK+W
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 275 PDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTIKISKVSFKNIRGTS 334
           P S     AS+MHF+DI M++V  PI+IDQ YCP+NQC  +VPS++K+SK+SFKNI+GTS
Sbjct: 241 PKSEPG-EASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 335 STPVAVKLVCSKSHPCEDVEIADIDLTYSGKEG 359
           +T  AVKLVCSKS PC  VE+ADIDLTYSGK G
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGG 332

BLAST of CmoCh05G008250 vs. ExPASy Swiss-Prot
Match: P35337 (Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 7.2e-110
Identity = 188/365 (51.51%), Postives = 262/365 (71.78%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           + +  +L+L +   +A+A     ++   G  PN+D+T A+  A+  AC +  PS+VL+PK
Sbjct: 5   LGIYTILVLCLLGYSANA-----EVFTAGGPPNSDITAAVLKAFTSACQAPAPSQVLIPK 64

Query: 61  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQG 120
           G ++LGE+ + GPCKSPIE  ++G ++     T+G D  +V E ++   L G G FDG+G
Sbjct: 65  GDFKLGETVMTGPCKSPIEFTLQGNVKTDGGSTQGKDRWVVFEKINGFKLNGGGTFDGEG 124

Query: 121 KAGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKI 180
            A W+ N+CH   ECKKLP++++F+F+ N+ +KD+TSLD+KNFH NV++GKN+TFD++KI
Sbjct: 125 NAAWKANNCHKTFECKKLPISVRFDFVDNAEIKDVTSLDAKNFHFNVISGKNMTFDNIKI 184

Query: 181 TAPEHSPNTDGIHLGDSEDVYILNTVIATGDDCISKNNH------SDVTCGPGHGISIGS 240
            AP  SPNTDGIHLG  E V ILNT IATGDDCIS  +         V CGPGHGIS+GS
Sbjct: 185 IAPAESPNTDGIHLGRCEGVKILNTKIATGDDCISVGDGMKNLLIEKVVCGPGHGISVGS 244

Query: 241 LGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIII 300
           LG+Y  E++V  +TVK C L GT+NG+RIK+WP +  +  A+ +HFEDI ++ V+NPI+I
Sbjct: 245 LGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAACTTTAAGIHFEDIILNKVSNPILI 304

Query: 301 DQEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTY 359
           DQEYCPWNQCN+  PSTIK+  ++F+NIRGTS    AVKL+CSK HPCE+VEI DI++ Y
Sbjct: 305 DQEYCPWNQCNKNKPSTIKLVDITFRNIRGTSENKDAVKLLCSKGHPCENVEIGDINIEY 364

BLAST of CmoCh05G008250 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.3e-108
Identity = 197/351 (56.13%), Postives = 248/351 (70.66%), Query Frame = 0

Query: 18  ATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSP 77
           A SGV DI+KFG KPN+D+ QAL  AW EACAS T +K+++P GTYQL    LKGPCK+P
Sbjct: 20  AQSGVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAP 79

Query: 78  IELLVEGTLQ--ASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQGKAGWEK----NDCHV 137
           IEL V+GT+Q  A P+  +G +      Y+DHLTL+G GVFDGQG   ++K    +    
Sbjct: 80  IELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSG 139

Query: 138 KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDG 197
           K    K+ MN  FNF+ NSIV+ +TS DSKNFH+ V   KN+TFD   ITAP  SPNTDG
Sbjct: 140 KNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDG 199

Query: 198 IHLGDSEDVYILNTVIATGDDCISKNNHS------DVTCGPGHGISIGSLGKYKTEKEVV 257
           IH+G S DV ILNT I TGDDC+S  + S       V CGPGHG+S+GSLGK+ TE+ V 
Sbjct: 200 IHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVE 259

Query: 258 GVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCN 317
           G+TVK C L  T NGVRIK+WPD+  +   SD+HFEDI M NV NP+IIDQEY PWNQC+
Sbjct: 260 GITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCS 319

Query: 318 RKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSG 356
           +K PS IK+SK+SFKN++GTS T   V L+CS + PC+ VE+ ++DL ++G
Sbjct: 320 KKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNG 370

BLAST of CmoCh05G008250 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.2e-104
Identity = 189/362 (52.21%), Postives = 256/362 (70.72%), Query Frame = 0

Query: 7   LLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLG 66
           L+LL      ++ +GVFDITK+GA  NAD+++AL +A+KEAC S +PS +++PKGT+ + 
Sbjct: 11  LVLLFLAHFGESQTGVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMN 70

Query: 67  ESRLKGPCKSPIELLVEGTLQA--SPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGKAGWE 126
           + +L+GPCKSP+EL ++ TL+A   P+  +    L +  +D  T++G G+ DGQ  A WE
Sbjct: 71  QVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAWE 130

Query: 127 KNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEH 186
              C   K+C KLP NL FN +TNS +KDIT+LDSK+FH+NV   KNLTF    ++AP +
Sbjct: 131 ---CKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPAN 190

Query: 187 SPNTDGIHLGDSEDVYILNTVIATGDDCISKNNHSD------VTCGPGHGISIGSLGKYK 246
           SPNTDGIH+  S  V I ++  +TGDDCIS  + ++      VTCGPGHGIS+GSLG   
Sbjct: 191 SPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNP 250

Query: 247 TEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYP--ASDMHFEDIEMDNVANPIIIDQE 306
            EK VVGV V+ C    T NGVRIK+WP    S+P   +D+HFEDI + NV+NP++IDQ 
Sbjct: 251 DEKPVVGVFVRNCTFTNTDNGVRIKTWP---ASHPGVVNDVHFEDIIVQNVSNPVVIDQV 310

Query: 307 YCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGK 359
           YCP+N+CN+ +PS +KISKVSF+NI+GTS T  AV L+ SK  PCE +E+ DID+TYSGK
Sbjct: 311 YCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGK 366

BLAST of CmoCh05G008250 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.5e-102
Identity = 184/367 (50.14%), Postives = 259/367 (70.57%), Query Frame = 0

Query: 2   ALIPLLLLLVSNATADATSGVFD-ITKFGAKPN--ADVTQALADAWKEACASATPSKVLV 61
           ++  LLLL +S   +   S  FD + KFGAK +   D+++   DAWKEACAS TPS V++
Sbjct: 10  SMFVLLLLFIS--ASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVI 69

Query: 62  PKGTYQLGESRLKGPCKSPIELLVEGTLQ--ASPTDTEGDGLLVMEYVDHLTLAGTGVFD 121
           PKGTY L +  L+GPCK+PIE+ V+GT+Q  A P+  +    +    V++  + G G+FD
Sbjct: 70  PKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFD 129

Query: 122 GQGKAGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDH 181
           GQG   +EKN C  ++   KLP+N++F+F+TN++++DITS DSK FHINV A KN+T + 
Sbjct: 130 GQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLER 189

Query: 182 LKITAPEHSPNTDGIHLGDSEDVYILNTVIATGDDCISKNNHS------DVTCGPGHGIS 241
           LKI AP+ SPNTDGIH+G SE V I+ + I TGDDCIS  + +      ++TCGPGHGIS
Sbjct: 190 LKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGIS 249

Query: 242 IGSLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANP 301
           IGSLGK++ E+ V G+ +  C +  T+NG RIK+WP        S++HFEDI M+NV++P
Sbjct: 250 IGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGAVSEIHFEDITMNNVSSP 309

Query: 302 IIIDQEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADID 358
           I+IDQ+YCPWN+C +   S +K+S +SFKNIRGTS+ P A+K +CS S PC++VE+ADID
Sbjct: 310 ILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADID 369

BLAST of CmoCh05G008250 vs. ExPASy TrEMBL
Match: A0A6J1EYY1 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439949 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.1e-201
Identity = 355/366 (96.99%), Postives = 355/366 (96.99%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK
Sbjct: 8   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQL ESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK
Sbjct: 68  GTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCIS------KNNHSDVTCGPGHGISIGSL 240
           APEHSPNTDGIHLGDSEDVYILNTVIATGDDCIS      K   SDVTCGPGHGISIGSL
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 360
           QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 367

BLAST of CmoCh05G008250 vs. ExPASy TrEMBL
Match: A0A6J1I9Y3 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472545 PE=3 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 7.7e-200
Identity = 351/366 (95.90%), Postives = 353/366 (96.45%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MALIPLLLLLVSNAT DATSGVFDIT FGAKPNADVTQALADAWKEACASATPSKVLVPK
Sbjct: 8   MALIPLLLLLVSNATVDATSGVFDITTFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTL+GTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLSGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCIS------KNNHSDVTCGPGHGISIGSL 240
           APEHSPNTDGIHLGDSEDVYILNTVIATGDDCIS      K   SDVTCGPGHGISIGSL
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDS VSYPASDMHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 360
           QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 367

BLAST of CmoCh05G008250 vs. ExPASy TrEMBL
Match: A0A6J1IE44 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111474737 PE=3 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 7.7e-200
Identity = 351/366 (95.90%), Postives = 353/366 (96.45%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MALIPLLLLLVSNAT DATSGVFDIT FGAKPNADVTQALADAWKEACASATPSKVLVPK
Sbjct: 8   MALIPLLLLLVSNATVDATSGVFDITTFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTL+GTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLSGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCIS------KNNHSDVTCGPGHGISIGSL 240
           APEHSPNTDGIHLGDSEDVYILNTVIATGDDCIS      K   SDVTCGPGHGISIGSL
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDS VSYPASDMHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 360
           QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 367

BLAST of CmoCh05G008250 vs. ExPASy TrEMBL
Match: A0A6J1G454 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111450616 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.5e-195
Identity = 343/366 (93.72%), Postives = 348/366 (95.08%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MAL+PLLLLLVSNAT DATSGVFDIT FGAKPNAD TQAL DAWKEACASATPSKVLVPK
Sbjct: 8   MALMPLLLLLVSNATVDATSGVFDITTFGAKPNADATQALVDAWKEACASATPSKVLVPK 67

Query: 61  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCIS------KNNHSDVTCGPGHGISIGSL 240
           AP +SPNTDGIHLGDSEDVYILNT IATGDDC+S      K   SDVTCGPGHGISIGSL
Sbjct: 188 APHNSPNTDGIHLGDSEDVYILNTAIATGDDCVSVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDS VSYPASDMHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 360
           QEYCPWNQCNRKVPS IKISKVSFKNIRGTSSTPVAVKLVCSKS+PCEDVEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSAIKISKVSFKNIRGTSSTPVAVKLVCSKSNPCEDVEIADIDLTYS 367

BLAST of CmoCh05G008250 vs. ExPASy TrEMBL
Match: A0A6J1ENZ5 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111436229 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.4e-195
Identity = 343/364 (94.23%), Postives = 349/364 (95.88%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK
Sbjct: 8   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQLG SRLKGPCKSPIELLVEGTLQASP+DTEGDGLLVMEYVDHLTLAGTGVFDGQGK
Sbjct: 68  GTYQLGASRLKGPCKSPIELLVEGTLQASPSDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCIS------KNNHSDVTCGPGHGISIGSL 240
           AP +SPNTDGIH+GDSEDVYILNT IATGDDCIS      K   SDVTCGPGHGISIGSL
Sbjct: 188 APHNSPNTDGIHMGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDS VSYPASDMHFEDIEM+NVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 359
           QEYCPWNQCNRKVPS IKISKVSFKNIRGTSSTPVAVKLVCSKS+PCE VEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSAIKISKVSFKNIRGTSSTPVAVKLVCSKSNPCEGVEIADIDLTYS 367

BLAST of CmoCh05G008250 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-110
Identity = 187/342 (54.68%), Postives = 255/342 (74.56%), Query Frame = 0

Query: 24  DITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSPIELLVE 83
           ++   G+   +D+TQAL  A+  AC S++PSKV++PKG ++LGE  ++GPCK+PIE+ ++
Sbjct: 23  EVFTIGSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQ 82

Query: 84  GTLQASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKECKKLPMNLK 143
           GT++A     +G +  +V   +D   L G G FDG+G A W  N+CH   ECKKLP++++
Sbjct: 83  GTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIR 142

Query: 144 FNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSEDVYIL 203
           F+FI NS ++DI+S+D+KNFHINVL  KN+T +++KI APE SPNTDGIHLG S+ V IL
Sbjct: 143 FDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKIL 202

Query: 204 NTVIATGDDCIS-----KNNHSD-VTCGPGHGISIGSLGKYKTEKEVVGVTVKKCKLIGT 263
           N+ I+TGDDCIS     KN H + VTCGPGHGIS+GSLG+Y  E++V G+ V  C L  T
Sbjct: 203 NSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQET 262

Query: 264 TNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTIKISKV 323
            NG+RIK+WP +  S  ASD+HFEDI + +V+NPI+IDQEYCPWNQCN++  STIK+  +
Sbjct: 263 DNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNI 322

Query: 324 SFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEG 359
           SFKNIRGTS    AVKL+CSK +PC++VEI DID+ Y+G +G
Sbjct: 323 SFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADG 364

BLAST of CmoCh05G008250 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 396.0 bits (1016), Expect = 3.3e-110
Identity = 191/362 (52.76%), Postives = 263/362 (72.65%), Query Frame = 0

Query: 4   IPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTY 63
           I  +L ++S     A + VF++   G  P +D+TQAL  A+  AC + T SKV++ KG +
Sbjct: 7   ISAVLFVISLLGFSANAEVFNV---GGSPGSDITQALLKAFTSACQAPTASKVVITKGEF 66

Query: 64  QLGESRLKGPCKSPIELLVEGTLQASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQGKAG 123
           +LGE  + GPCK+P+E+ ++GTL+A     +G +  +V   ++   L G G+FDG+G A 
Sbjct: 67  KLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVFLRINGFKLNGGGIFDGEGNAA 126

Query: 124 WEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAP 183
           W  N+CH   ECKKLP++++F+F+ N+ ++DI+S+D+KNFHINVL  KN+TFD++K+ AP
Sbjct: 127 WRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAP 186

Query: 184 EHSPNTDGIHLGDSEDVYILNTVIATGDDCIS-----KNNH-SDVTCGPGHGISIGSLGK 243
             SPNTDGIHLG SE V ILN+ IATGDDCIS     KN H  +V CGPGHGIS+GSLG+
Sbjct: 187 AESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGR 246

Query: 244 YKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQE 303
           Y  E++V G+TV  C L GT NG+RIK+WP +  +  AS +HFE+I ++NV+NPI+IDQE
Sbjct: 247 YVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSNPILIDQE 306

Query: 304 YCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGK 359
           YCPWNQCN++ PSTIK+  +SFKNIRGTS    AVKL+CSK+HPC +VEI +I+L Y G 
Sbjct: 307 YCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGA 365

BLAST of CmoCh05G008250 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 3.2e-105
Identity = 174/347 (50.14%), Postives = 250/347 (72.05%), Query Frame = 0

Query: 19  TSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSPI 78
           ++   ++ +  + P +D+TQAL  A+  AC S TP KV++PKG ++LGE  + GPCKSP+
Sbjct: 19  SANAHEVLRLTSSPGSDITQALLRAFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPV 78

Query: 79  ELLVEGTLQASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKECKKL 138
           E+ + GT+ A      G +  +V + +D   L G G FDG+G A W  N+CH   ECKKL
Sbjct: 79  EITLLGTVLADGNSIHGKEKWVVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKL 138

Query: 139 PMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSE 198
           P++++F+F+TN+ ++DI+S+D+KNFHINV+  KN+TFD++KI AP  SPNTDGIHLG S 
Sbjct: 139 PISIRFDFVTNAEIRDISSIDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSV 198

Query: 199 DVYILNTVIATGDDCISKNNH------SDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKC 258
            V I+N+ I+TGDDC+S  +        +V CGPGHGIS+GSLG+Y  E++V G+ V  C
Sbjct: 199 GVSIINSRISTGDDCVSVGDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINC 258

Query: 259 KLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTI 318
            L  T NG+RIK+WP +  S  AS++HFE+I + NV+NPI+IDQEYCPWNQCN++  S+I
Sbjct: 259 TLQETDNGLRIKTWPSAACSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSI 318

Query: 319 KISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEG 359
           K++ +SF+ IRGTS    AVKL+CSK +PCE+V++ DI++ Y+G +G
Sbjct: 319 KLANISFRRIRGTSGNKDAVKLLCSKGYPCENVQVGDINIQYTGADG 365

BLAST of CmoCh05G008250 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 1.5e-102
Identity = 171/347 (49.28%), Postives = 245/347 (70.61%), Query Frame = 0

Query: 18  ATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSP 77
           A + +F+I    + P +D+T AL  A+ EAC   T S V++PKG Y+LGE  + GPCK+P
Sbjct: 21  ANAKIFNI---NSPPGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAP 80

Query: 78  IELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKECKKL 137
           I + + GT++A       +  +    ++   L G GVFDG+G A W  N+CH    CKKL
Sbjct: 81  IRIALLGTVKADGNANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKL 140

Query: 138 PMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSE 197
           P++++F+F+T++ ++ ITSLD+K+FHINV+  KN+TF+ +KI AP  SPNTDGIH+G S+
Sbjct: 141 PISIRFDFVTDAKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSD 200

Query: 198 DVYILNTVIATGDDCISKNNH------SDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKC 257
            + I+N+ I+TGDDC+S  +         VTCGPGHGISIGSLG+Y  E+ V G+ +  C
Sbjct: 201 GIKIINSFISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINC 260

Query: 258 KLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTI 317
            L  T NG+RIK+WP +  +  ASD+HFE+I + NV+NPI+IDQEYCPWNQCN++ PSTI
Sbjct: 261 TLQETDNGLRIKTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTI 320

Query: 318 KISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEG 359
           K++ +SFK IRGTS    AVKL+CSK +PC++VE+ D+++ Y+G +G
Sbjct: 321 KLANISFKKIRGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADG 364

BLAST of CmoCh05G008250 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 7.4e-86
Identity = 166/347 (47.84%), Postives = 221/347 (63.69%), Query Frame = 0

Query: 24  DITKFGAKPN--ADVTQALADAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSPIELL 83
           D+   GAK +   D + A A AWKEACA+   S + VPKG Y +     KGPCK P+ L 
Sbjct: 72  DVKASGAKGDGKTDDSAAFAAAWKEACAAG--STITVPKGEYLVESLEFKGPCKGPVTLE 131

Query: 84  VEGTLQAS---PTDTEGDGLLVMEYVDHLTLAGT-GVFDGQGKAGWEKNDCHVKKECKKL 143
           + G  +A     T     G +  E +   TL G   +FDGQG   W+ NDC    +C  L
Sbjct: 132 LNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSL 191

Query: 144 PMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSE 203
           P+N++F  +TNS +  ITS +SK FH+N+L  KN+T   + I AP  S NTDGIH+G S 
Sbjct: 192 PINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSN 251

Query: 204 DVYILNTVIATGDDCISKNNHSD------VTCGPGHGISIGSLGKYKTEKEVVGVTVKKC 263
            V ++   I TGDDC+S  + ++      V CGPGHGISIGSLG+Y  E+ V GVTV+KC
Sbjct: 252 GVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKC 311

Query: 264 KLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTI 323
            +  T NGVRIK+WP S     AS++ FEDI MDNV+ P++IDQEYCP+  C   VPS +
Sbjct: 312 LIKNTDNGVRIKTWPGSPPGI-ASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKV 371

Query: 324 KISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEG 359
           K+S V+ KNI+GTS+T VAVKL+CSK  PC ++ ++DI+L ++GKEG
Sbjct: 372 KLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEG 415

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P245485.5e-11059.76Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
P353377.2e-11051.51Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1[more]
Q403121.3e-10856.13Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Q059671.2e-10452.21Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
Q397862.5e-10250.14Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EYY13.1e-20196.99exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439949 PE=3 SV=... [more]
A0A6J1I9Y37.7e-20095.90exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472545 PE=3 SV=1[more]
A0A6J1IE447.7e-20095.90exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111474737 PE=3 SV=1[more]
A0A6J1G4541.5e-19593.72exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111450616 PE=3 SV=... [more]
A0A6J1ENZ54.4e-19594.23exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111436229 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G07820.11.5e-11054.68Pectin lyase-like superfamily protein [more]
AT3G07840.13.3e-11052.76Pectin lyase-like superfamily protein [more]
AT3G07830.13.2e-10550.14Pectin lyase-like superfamily protein [more]
AT5G48140.11.5e-10249.28Pectin lyase-like superfamily protein [more]
AT3G14040.17.4e-8647.84Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 196..233
e-value: 1300.0
score: 6.5
coord: 169..195
e-value: 970.0
score: 7.3
coord: 243..264
e-value: 19.0
score: 14.2
coord: 274..295
e-value: 6200.0
score: 1.6
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 48..356
e-value: 6.2E-98
score: 327.8
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 220..233
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 10..360
e-value: 2.3E-99
score: 335.2
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 9..356
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 9..356
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 11..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G008250.1CmoCh05G008250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity