CmoCh05G008230 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G008230
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionexopolygalacturonase-like
LocationCmo_Chr05: 4984748 .. 4986437 (+)
RNA-Seq ExpressionCmoCh05G008230
SyntenyCmoCh05G008230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGATATCAAAGTTTATGGCATTAATACCACTACTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACAAAATTTGGTGCCAAACCTAATGCTGATGTTACTCAAGTACGTGTACATTTGTTTAAGTTGATAAACCAAAATTTCATTTTGTACAAAATATTTCTCTCAAATGAAATGGTTGAATTCACAGGCTTTAGCAGATGCATGGAAGGAAGCATGTGCGTCGGCTACTCCAAGTAAAGTATTAGTTCCAAAAGGAACTTATCAATTAGAAGAATCCAGGCTCAAAGGTCCATGTAAAAGTCCTATTGAGCTTCTAGTTGAAGGAACATTACAAGCTTCTCCTACCGACACGGAAGGAGATGGGTTGCTTGTTATGGAATACGTCGATCATTTGACATTGGCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTGGTTGGGAAAAGAATGATTGCCACGTGAAGAAAGAATGCAAAAAGCTTCCCATGGTAAATGCTCATACTACTTTAATTATAAAAAGGACCCATACTCTGGGTTAGCATTGTATGTTTTAACACGGGAGTGGTTTTTTTTTGTTTGTTTAAAATGAGCAGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGGCCGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGAGCATAGTCCCAACACCGATGGAATTCACTTGGGTGATTCAGAAGACGTTTACATTCTCAATACTGTAATCGCAACGGGAGATGATTGTATATCTGTAGGATACACTAACAGAAAAATAACCATTAGTGATGTCACTTGCGGACCGGGACATGGCATTAGCATAGGCAGCTTGGGCAAGTATAAAACTGAAAAAGAAGTGGTAGGAGTTACAGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGCCAGATTCTGTCGTCTCATACCCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTATGTATTAAAATCGTCGAACCTCGTTGGTTTCCAACTTATTATCTTTTGAACAAGTATTAATTCATGTTTGTTTTGAACATCCAGGTTCCATCAACAATTAAGATTAGCAAAGTTAGTTTCAAGAATATCAGGGGCACATCTTCTACTCCCGTTGCAGTCAAACTTGTTTGCAGTAAAAGTCATCCATGTGAGGATGTGGAAATTGCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGCCAATTGTGTCCGAGTGTGCAAATGTGAAACCGACCGTTTCTGGAAAGCAAAACCCTCCCATCTGTGCTAAACCAGCGCCACCTGAGACCACTCCGTCAACTGATTGAATAATCTTTTAACGCCGACAAATGTTTCAAGCCATTCTTCCTATCATTTTTATTTTAGCTTTCAAAATTCGGTTTACAACATGCCACGATGTTGGGCCTGTTTTCAATAATTGTATTTTTTACATTTAGGAGAAACAATACTTAACGATTCTATTTTTCTAGTGTAATACATAATTTACATTCCTTTGAAGGAGGAG

mRNA sequence

ATTGATATCAAAGTTTATGGCATTAATACCACTACTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACAAAATTTGGTGCCAAACCTAATGCTGATGTTACTCAAGCTTTAGCAGATGCATGGAAGGAAGCATGTGCGTCGGCTACTCCAAGTAAAGTATTAGTTCCAAAAGGAACTTATCAATTAGAAGAATCCAGGCTCAAAGGTCCATGTAAAAGTCCTATTGAGCTTCTAGTTGAAGGAACATTACAAGCTTCTCCTACCGACACGGAAGGAGATGGGTTGCTTGTTATGGAATACGTCGATCATTTGACATTGGCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTGGTTGGGAAAAGAATGATTGCCACGTGAAGAAAGAATGCAAAAAGCTTCCCATGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGGCCGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGAGCATAGTCCCAACACCGATGGAATTCACTTGGGTGATTCAGAAGACGTTTACATTCTCAATACTGTAATCGCAACGGGAGATGATTGTATATCTGTAGGATACACTAACAGAAAAATAACCATTAGTGATGTCACTTGCGGACCGGGACATGGCATTAGCATAGGCAGCTTGGGCAAGTATAAAACTGAAAAAGAAGTGGTAGGAGTTACAGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGCCAGATTCTGTCGTCTCATACCCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTTCCATCAACAATTAAGATTAGCAAAGTTAGTTTCAAGAATATCAGGGGCACATCTTCTACTCCCGTTGCAGTCAAACTTGTTTGCAGTAAAAGTCATCCATGTGAGGATGTGGAAATTGCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGCCAATTGTGTCCGAGTGTGCAAATGTGAAACCGACCGTTTCTGGAAAGCAAAACCCTCCCATCTGTGCTAAACCAGCGCCACCTGAGACCACTCCGTCAACTGATTGAATAATCTTTTAACGCCGACAAATGTTTCAAGCCATTCTTCCTATCATTTTTATTTTAGCTTTCAAAATTCGGTTTACAACATGCCACGATGTTGGGCCTGTTTTCAATAATTGTATTTTTTACATTTAGGAGAAACAATACTTAACGATTCTATTTTTCTAGTGTAATACATAATTTACATTCCTTTGAAGGAGGAG

Coding sequence (CDS)

ATGGCATTAATACCACTACTGCTATTGTTGGTGTCTAATGCTACCGCTGATGCTACTAGCGGTGTTTTTGATATTACAAAATTTGGTGCCAAACCTAATGCTGATGTTACTCAAGCTTTAGCAGATGCATGGAAGGAAGCATGTGCGTCGGCTACTCCAAGTAAAGTATTAGTTCCAAAAGGAACTTATCAATTAGAAGAATCCAGGCTCAAAGGTCCATGTAAAAGTCCTATTGAGCTTCTAGTTGAAGGAACATTACAAGCTTCTCCTACCGACACGGAAGGAGATGGGTTGCTTGTTATGGAATACGTCGATCATTTGACATTGGCTGGTACTGGAGTTTTCGATGGCCAAGGAAAAGCTGGTTGGGAAAAGAATGATTGCCACGTGAAGAAAGAATGCAAAAAGCTTCCCATGAATTTGAAGTTCAATTTCATCACAAATTCGATAGTGAAGGACATAACTTCATTGGATAGCAAGAATTTTCATATCAATGTTTTGGCCGGCAAGAATCTTACCTTCGATCACCTGAAAATTACTGCACCAGAGCATAGTCCCAACACCGATGGAATTCACTTGGGTGATTCAGAAGACGTTTACATTCTCAATACTGTAATCGCAACGGGAGATGATTGTATATCTGTAGGATACACTAACAGAAAAATAACCATTAGTGATGTCACTTGCGGACCGGGACATGGCATTAGCATAGGCAGCTTGGGCAAGTATAAAACTGAAAAAGAAGTGGTAGGAGTTACAGTGAAGAAATGCAAATTAATCGGTACTACAAATGGTGTCAGGATCAAATCATGGCCAGATTCTGTCGTCTCATACCCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGATAATGTTGCTAACCCTATCATCATAGATCAAGAATATTGTCCATGGAATCAATGCAACCGAAAGGTTCCATCAACAATTAAGATTAGCAAAGTTAGTTTCAAGAATATCAGGGGCACATCTTCTACTCCCGTTGCAGTCAAACTTGTTTGCAGTAAAAGTCATCCATGTGAGGATGTGGAAATTGCTGATATTGACCTCACTTATAGTGGTAAAGAAGGGCCAATTGTGTCCGAGTGTGCAAATGTGAAACCGACCGTTTCTGGAAAGCAAAACCCTCCCATCTGTGCTAAACCAGCGCCACCTGAGACCACTCCGTCAACTGATTGA

Protein sequence

MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD
Homology
BLAST of CmoCh05G008230 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.0e-120
Identity = 212/359 (59.05%), Postives = 271/359 (75.49%), Query Frame = 0

Query: 35  DVTQALADAWKEACASATPSKVLVPKGTYQLEESRLKGPCKSPIELLVEGTLQ--ASPTD 94
           D TQAL  AWKEACASA+PS +LVPKG + +    L+GPCKS I L ++GTL+  A P+ 
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 95  TEGDGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKE-CKKLPMNLKFNFITNSIV 154
            +G G + +  +D LT+ G GVFDGQGK+ W +NDCH     CK L MNL+   +TNSI+
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 155 KDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSEDVYILNTVIATGDD 214
           +D+T+LDSKNFH+NV+  KNLTF+  KI+A E S NTDGIH+G S+ V I+NT I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 215 CISVGYTNRKITISDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSW 274
           CIS+G  ++ I I+++TCGPGHGIS+GSLG+YK E+ VVG+ VK C + G+ NGVRIK+W
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 275 PDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTIKISKVSFKNIRGTS 334
           P S     AS+MHF+DI M++V  PI+IDQ YCP+NQC  +VPS++K+SK+SFKNI+GTS
Sbjct: 241 PKSEPG-EASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 335 STPVAVKLVCSKSHPCEDVEIADIDLTYSGKEGPIVSECANVKPTVSGKQNPPICAKPA 391
           +T  AVKLVCSKS PC  VE+ADIDLTYSGK GP  S C N+KPT+ GKQ P IC+  A
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICSGSA 358

BLAST of CmoCh05G008230 vs. ExPASy Swiss-Prot
Match: P35337 (Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 3.2e-119
Identity = 202/390 (51.79%), Postives = 278/390 (71.28%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           + +  +L+L +   +A+A     ++   G  PN+D+T A+  A+  AC +  PS+VL+PK
Sbjct: 5   LGIYTILVLCLLGYSANA-----EVFTAGGPPNSDITAAVLKAFTSACQAPAPSQVLIPK 64

Query: 61  GTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQG 120
           G ++L E+ + GPCKSPIE  ++G ++     T+G D  +V E ++   L G G FDG+G
Sbjct: 65  GDFKLGETVMTGPCKSPIEFTLQGNVKTDGGSTQGKDRWVVFEKINGFKLNGGGTFDGEG 124

Query: 121 KAGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKI 180
            A W+ N+CH   ECKKLP++++F+F+ N+ +KD+TSLD+KNFH NV++GKN+TFD++KI
Sbjct: 125 NAAWKANNCHKTFECKKLPISVRFDFVDNAEIKDVTSLDAKNFHFNVISGKNMTFDNIKI 184

Query: 181 TAPEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGS 240
            AP  SPNTDGIHLG  E V ILNT IATGDDCISVG   + + I  V CGPGHGIS+GS
Sbjct: 185 IAPAESPNTDGIHLGRCEGVKILNTKIATGDDCISVGDGMKNLLIEKVVCGPGHGISVGS 244

Query: 241 LGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIII 300
           LG+Y  E++V  +TVK C L GT+NG+RIK+WP +  +  A+ +HFEDI ++ V+NPI+I
Sbjct: 245 LGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAACTTTAAGIHFEDIILNKVSNPILI 304

Query: 301 DQEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTY 360
           DQEYCPWNQCN+  PSTIK+  ++F+NIRGTS    AVKL+CSK HPCE+VEI DI++ Y
Sbjct: 305 DQEYCPWNQCNKNKPSTIKLVDITFRNIRGTSENKDAVKLLCSKGHPCENVEIGDINIEY 364

Query: 361 SGKEGPIVSECANVKPTVSGKQNPPICAKP 390
           +G +GP   EC NV P + G QNP  C  P
Sbjct: 365 TGPDGPPTFECTNVTPKLVGAQNPKACVGP 389

BLAST of CmoCh05G008230 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.0e-117
Identity = 214/389 (55.01%), Postives = 275/389 (70.69%), Query Frame = 0

Query: 18  ATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLEESRLKGPCKSP 77
           A SGV DI+KFG KPN+D+ QAL  AW EACAS T +K+++P GTYQL    LKGPCK+P
Sbjct: 20  AQSGVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAP 79

Query: 78  IELLVEGTLQ--ASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQGKAGWEK----NDCHV 137
           IEL V+GT+Q  A P+  +G +      Y+DHLTL+G GVFDGQG   ++K    +    
Sbjct: 80  IELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSG 139

Query: 138 KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDG 197
           K    K+ MN  FNF+ NSIV+ +TS DSKNFH+ V   KN+TFD   ITAP  SPNTDG
Sbjct: 140 KNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDG 199

Query: 198 IHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGKYKTEKEVV 257
           IH+G S DV ILNT I TGDDC+S+G  +++IT+  V CGPGHG+S+GSLGK+ TE+ V 
Sbjct: 200 IHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVE 259

Query: 258 GVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCN 317
           G+TVK C L  T NGVRIK+WPD+  +   SD+HFEDI M NV NP+IIDQEY PWNQC+
Sbjct: 260 GITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCS 319

Query: 318 RKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEGPIVSEC 377
           +K PS IK+SK+SFKN++GTS T   V L+CS + PC+ VE+ ++DL ++G   P  ++C
Sbjct: 320 KKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNG--APTTAKC 379

Query: 378 ANVKPTVSGKQNPPICAKP-APPETTPST 399
            NVKP V+G    P+C  P AP  +T +T
Sbjct: 380 TNVKPLVTG--TAPVCQAPGAPAASTTAT 404

BLAST of CmoCh05G008230 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 3.9e-117
Identity = 206/388 (53.09%), Postives = 277/388 (71.39%), Query Frame = 0

Query: 7   LLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLE 66
           L+LL      ++ +GVFDITK+GA  NAD+++AL +A+KEAC S +PS +++PKGT+ + 
Sbjct: 11  LVLLFLAHFGESQTGVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMN 70

Query: 67  ESRLKGPCKSPIELLVEGTLQA--SPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGKAGWE 126
           + +L+GPCKSP+EL ++ TL+A   P+  +    L +  +D  T++G G+ DGQ  A WE
Sbjct: 71  QVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAWE 130

Query: 127 KNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEH 186
              C   K+C KLP NL FN +TNS +KDIT+LDSK+FH+NV   KNLTF    ++AP +
Sbjct: 131 ---CKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPAN 190

Query: 187 SPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGKYK 246
           SPNTDGIH+  S  V I ++  +TGDDCISVG    ++ I+ VTCGPGHGIS+GSLG   
Sbjct: 191 SPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNP 250

Query: 247 TEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYP--ASDMHFEDIEMDNVANPIIIDQE 306
            EK VVGV V+ C    T NGVRIK+WP    S+P   +D+HFEDI + NV+NP++IDQ 
Sbjct: 251 DEKPVVGVFVRNCTFTNTDNGVRIKTWP---ASHPGVVNDVHFEDIIVQNVSNPVVIDQV 310

Query: 307 YCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGK 366
           YCP+N+CN+ +PS +KISKVSF+NI+GTS T  AV L+ SK  PCE +E+ DID+TYSGK
Sbjct: 311 YCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGK 370

Query: 367 EGPIVSECANVKPTVSGKQNPPICAKPA 391
           EGP  S C N+KP++ GKQNPP+C   A
Sbjct: 371 EGPAKSSCENIKPSLKGKQNPPVCTASA 392

BLAST of CmoCh05G008230 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 4.5e-113
Identity = 204/401 (50.87%), Postives = 282/401 (70.32%), Query Frame = 0

Query: 2   ALIPLLLLLVSNATADATSGVFD-ITKFGAKPN--ADVTQALADAWKEACASATPSKVLV 61
           ++  LLLL +S   +   S  FD + KFGAK +   D+++   DAWKEACAS TPS V++
Sbjct: 10  SMFVLLLLFIS--ASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVI 69

Query: 62  PKGTYQLEESRLKGPCKSPIELLVEGTLQ--ASPTDTEGDGLLVMEYVDHLTLAGTGVFD 121
           PKGTY L +  L+GPCK+PIE+ V+GT+Q  A P+  +    +    V++  + G G+FD
Sbjct: 70  PKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFD 129

Query: 122 GQGKAGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDH 181
           GQG   +EKN C  ++   KLP+N++F+F+TN++++DITS DSK FHINV A KN+T + 
Sbjct: 130 GQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLER 189

Query: 182 LKITAPEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGIS 241
           LKI AP+ SPNTDGIH+G SE V I+ + I TGDDCIS+G   + + I ++TCGPGHGIS
Sbjct: 190 LKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGIS 249

Query: 242 IGSLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANP 301
           IGSLGK++ E+ V G+ +  C +  T+NG RIK+WP        S++HFEDI M+NV++P
Sbjct: 250 IGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGAVSEIHFEDITMNNVSSP 309

Query: 302 IIIDQEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADID 361
           I+IDQ+YCPWN+C +   S +K+S +SFKNIRGTS+ P A+K +CS S PC++VE+ADID
Sbjct: 310 ILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADID 369

Query: 362 LTYSGKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPS 398
           + ++G E P  S+C NVKP  SGK NP  C+ P P   TPS
Sbjct: 370 IKHNGAE-PATSQCLNVKPITSGKLNPIPCSGPVP--KTPS 404

BLAST of CmoCh05G008230 vs. ExPASy TrEMBL
Match: A0A6J1EYY1 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439949 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 2.7e-230
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK
Sbjct: 8   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  GTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK
Sbjct: 68  GTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 240
           APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 360
           QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 367

Query: 361 GKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD 400
           GKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD
Sbjct: 368 GKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD 406

BLAST of CmoCh05G008230 vs. ExPASy TrEMBL
Match: A0A6J1I9Y3 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472545 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 4.8e-227
Identity = 392/399 (98.25%), Postives = 395/399 (99.00%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MALIPLLLLLVSNAT DATSGVFDIT FGAKPNADVTQALADAWKEACASATPSKVLVPK
Sbjct: 8   MALIPLLLLLVSNATVDATSGVFDITTFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  GTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQL ESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTL+GTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLSGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 240
           APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL
Sbjct: 188 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDS VSYPASDMHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 360
           QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 367

Query: 361 GKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD 400
           GKEGPIVSECANVKPT+SGKQNPPICAKPAPPETTPSTD
Sbjct: 368 GKEGPIVSECANVKPTISGKQNPPICAKPAPPETTPSTD 406

BLAST of CmoCh05G008230 vs. ExPASy TrEMBL
Match: A0A6J1G454 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111450616 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 9.4e-223
Identity = 384/399 (96.24%), Postives = 390/399 (97.74%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MAL+PLLLLLVSNAT DATSGVFDIT FGAKPNAD TQAL DAWKEACASATPSKVLVPK
Sbjct: 8   MALMPLLLLLVSNATVDATSGVFDITTFGAKPNADATQALVDAWKEACASATPSKVLVPK 67

Query: 61  GTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQL ESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK
Sbjct: 68  GTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 240
           AP +SPNTDGIHLGDSEDVYILNT IATGDDC+SVGYTNRKITISDVTCGPGHGISIGSL
Sbjct: 188 APHNSPNTDGIHLGDSEDVYILNTAIATGDDCVSVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDS VSYPASDMHFEDIEMDNVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMDNVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 360
           QEYCPWNQCNRKVPS IKISKVSFKNIRGTSSTPVAVKLVCSKS+PCEDVEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSAIKISKVSFKNIRGTSSTPVAVKLVCSKSNPCEDVEIADIDLTYS 367

Query: 361 GKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD 400
           GKEGPIVSECANVKPT+SGKQNPPICAKPAPPETTPSTD
Sbjct: 368 GKEGPIVSECANVKPTISGKQNPPICAKPAPPETTPSTD 406

BLAST of CmoCh05G008230 vs. ExPASy TrEMBL
Match: A0A6J1ENZ5 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111436229 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 4.7e-222
Identity = 384/399 (96.24%), Postives = 391/399 (97.99%), Query Frame = 0

Query: 1   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 60
           MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK
Sbjct: 8   MALIPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPK 67

Query: 61  GTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 120
           GTYQL  SRLKGPCKSPIELLVEGTLQASP+DTEGDGLLVMEYVDHLTLAGTGVFDGQGK
Sbjct: 68  GTYQLGASRLKGPCKSPIELLVEGTLQASPSDTEGDGLLVMEYVDHLTLAGTGVFDGQGK 127

Query: 121 AGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 180
           AGWEKNDCH+KKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT
Sbjct: 128 AGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKIT 187

Query: 181 APEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 240
           AP +SPNTDGIH+GDSEDVYILNT IATGDDCISVGYTNRKITISDVTCGPGHGISIGSL
Sbjct: 188 APHNSPNTDGIHMGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGSL 247

Query: 241 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIID 300
           GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDS VSYPASDMHFEDIEM+NVANPIIID
Sbjct: 248 GKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIIID 307

Query: 301 QEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYS 360
           QEYCPWNQCNRKVPS IKISKVSFKNIRGTSSTPVAVKLVCSKS+PCE VEIADIDLTYS
Sbjct: 308 QEYCPWNQCNRKVPSAIKISKVSFKNIRGTSSTPVAVKLVCSKSNPCEGVEIADIDLTYS 367

Query: 361 GKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD 400
           GKEGP VSECANVKPT+SGKQNPPICAKPAPPETTPSTD
Sbjct: 368 GKEGPAVSECANVKPTISGKQNPPICAKPAPPETTPSTD 406

BLAST of CmoCh05G008230 vs. ExPASy TrEMBL
Match: A0A6J1I8V2 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472559 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.0e-221
Identity = 385/401 (96.01%), Postives = 392/401 (97.76%), Query Frame = 0

Query: 1   MALIP--LLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLV 60
           MALIP  LLLLLVS+ATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLV
Sbjct: 8   MALIPLLLLLLLVSDATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLV 67

Query: 61  PKGTYQLEESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQ 120
           PKGTYQL  SRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTL GTGVFDGQ
Sbjct: 68  PKGTYQLGASRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYVDHLTLTGTGVFDGQ 127

Query: 121 GKAGWEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLK 180
           GKAGWEKNDCH+KK CKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLK
Sbjct: 128 GKAGWEKNDCHLKKICKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLK 187

Query: 181 ITAPEHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIG 240
           ITAPE+SPNTDGIHLGDSEDVYILNT IATGDDCISVGYTNRKITISDVTCGPGHGISIG
Sbjct: 188 ITAPENSPNTDGIHLGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIG 247

Query: 241 SLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPII 300
           SLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDS VSYPASDMHFEDIEM+NVANPII
Sbjct: 248 SLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPII 307

Query: 301 IDQEYCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLT 360
           IDQEYCPWNQCNRKVPSTIKISKVSFKNI+GTSSTPVAVKLVCSKSHPCEDVEIADIDLT
Sbjct: 308 IDQEYCPWNQCNRKVPSTIKISKVSFKNIKGTSSTPVAVKLVCSKSHPCEDVEIADIDLT 367

Query: 361 YSGKEGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD 400
           YSGK+GPIVSECANVKP +SGKQNPPICAKPAPPETTPSTD
Sbjct: 368 YSGKQGPIVSECANVKPKISGKQNPPICAKPAPPETTPSTD 408

BLAST of CmoCh05G008230 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 425.2 bits (1092), Expect = 5.6e-119
Identity = 196/368 (53.26%), Postives = 270/368 (73.37%), Query Frame = 0

Query: 24  DITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLEESRLKGPCKSPIELLVE 83
           ++   G+   +D+TQAL  A+  AC S++PSKV++PKG ++L E  ++GPCK+PIE+ ++
Sbjct: 23  EVFTIGSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQ 82

Query: 84  GTLQASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKECKKLPMNLK 143
           GT++A     +G +  +V   +D   L G G FDG+G A W  N+CH   ECKKLP++++
Sbjct: 83  GTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIR 142

Query: 144 FNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSEDVYIL 203
           F+FI NS ++DI+S+D+KNFHINVL  KN+T +++KI APE SPNTDGIHLG S+ V IL
Sbjct: 143 FDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKIL 202

Query: 204 NTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKCKLIGT 263
           N+ I+TGDDCISVG   + + +  VTCGPGHGIS+GSLG+Y  E++V G+ V  C L  T
Sbjct: 203 NSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQET 262

Query: 264 TNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTIKISKV 323
            NG+RIK+WP +  S  ASD+HFEDI + +V+NPI+IDQEYCPWNQCN++  STIK+  +
Sbjct: 263 DNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNI 322

Query: 324 SFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEGPIVSECANVKPTVSGKQN 383
           SFKNIRGTS    AVKL+CSK +PC++VEI DID+ Y+G +GP    C+NV P + G Q+
Sbjct: 323 SFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQS 382

Query: 384 PPICAKPA 391
           P  C+ PA
Sbjct: 383 PKACSAPA 390

BLAST of CmoCh05G008230 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 421.0 bits (1081), Expect = 1.1e-117
Identity = 202/397 (50.88%), Postives = 281/397 (70.78%), Query Frame = 0

Query: 4   IPLLLLLVSNATADATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTY 63
           I  +L ++S     A + VF++   G  P +D+TQAL  A+  AC + T SKV++ KG +
Sbjct: 7   ISAVLFVISLLGFSANAEVFNV---GGSPGSDITQALLKAFTSACQAPTASKVVITKGEF 66

Query: 64  QLEESRLKGPCKSPIELLVEGTLQASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQGKAG 123
           +L E  + GPCK+P+E+ ++GTL+A     +G +  +V   ++   L G G+FDG+G A 
Sbjct: 67  KLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVFLRINGFKLNGGGIFDGEGNAA 126

Query: 124 WEKNDCHVKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAP 183
           W  N+CH   ECKKLP++++F+F+ N+ ++DI+S+D+KNFHINVL  KN+TFD++K+ AP
Sbjct: 127 WRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAP 186

Query: 184 EHSPNTDGIHLGDSEDVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGK 243
             SPNTDGIHLG SE V ILN+ IATGDDCISVG   + + + +V CGPGHGIS+GSLG+
Sbjct: 187 AESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGR 246

Query: 244 YKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQE 303
           Y  E++V G+TV  C L GT NG+RIK+WP +  +  AS +HFE+I ++NV+NPI+IDQE
Sbjct: 247 YVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSNPILIDQE 306

Query: 304 YCPWNQCNRKVPSTIKISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGK 363
           YCPWNQCN++ PSTIK+  +SFKNIRGTS    AVKL+CSK+HPC +VEI +I+L Y G 
Sbjct: 307 YCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGA 366

Query: 364 EGPIVSECANVKPTVSGKQNPPICAKPAPPETTPSTD 400
           +GP    C+NV P + G QNP  C+    P+T    D
Sbjct: 367 DGPPTFMCSNVSPKLVGTQNPKACSAGPTPKTAAKGD 400

BLAST of CmoCh05G008230 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 402.5 bits (1033), Expect = 3.9e-112
Identity = 188/379 (49.60%), Postives = 268/379 (70.71%), Query Frame = 0

Query: 19  TSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLEESRLKGPCKSPI 78
           ++   ++ +  + P +D+TQAL  A+  AC S TP KV++PKG ++L E  + GPCKSP+
Sbjct: 19  SANAHEVLRLTSSPGSDITQALLRAFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPV 78

Query: 79  ELLVEGTLQASPTDTEG-DGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKECKKL 138
           E+ + GT+ A      G +  +V + +D   L G G FDG+G A W  N+CH   ECKKL
Sbjct: 79  EITLLGTVLADGNSIHGKEKWVVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKL 138

Query: 139 PMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSE 198
           P++++F+F+TN+ ++DI+S+D+KNFHINV+  KN+TFD++KI AP  SPNTDGIHLG S 
Sbjct: 139 PISIRFDFVTNAEIRDISSIDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSV 198

Query: 199 DVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKC 258
            V I+N+ I+TGDDC+SVG     + + +V CGPGHGIS+GSLG+Y  E++V G+ V  C
Sbjct: 199 GVSIINSRISTGDDCVSVGDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINC 258

Query: 259 KLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTI 318
            L  T NG+RIK+WP +  S  AS++HFE+I + NV+NPI+IDQEYCPWNQCN++  S+I
Sbjct: 259 TLQETDNGLRIKTWPSAACSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSI 318

Query: 319 KISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEGPIVSECANVKPTV 378
           K++ +SF+ IRGTS    AVKL+CSK +PCE+V++ DI++ Y+G +GP    C+NV+P +
Sbjct: 319 KLANISFRRIRGTSGNKDAVKLLCSKGYPCENVQVGDINIQYTGADGPATFMCSNVRPKL 378

Query: 379 SGKQNPPICAKPAPPETTP 397
            G Q P  C  P P  T P
Sbjct: 379 VGTQFPKACNTP-PVLTQP 396

BLAST of CmoCh05G008230 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 399.1 bits (1024), Expect = 4.3e-111
Identity = 184/378 (48.68%), Postives = 265/378 (70.11%), Query Frame = 0

Query: 18  ATSGVFDITKFGAKPNADVTQALADAWKEACASATPSKVLVPKGTYQLEESRLKGPCKSP 77
           A + +F+I    + P +D+T AL  A+ EAC   T S V++PKG Y+L E  + GPCK+P
Sbjct: 21  ANAKIFNI---NSPPGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAP 80

Query: 78  IELLVEGTLQASPTDTEGDGLLVMEYVDHLTLAGTGVFDGQGKAGWEKNDCHVKKECKKL 137
           I + + GT++A       +  +    ++   L G GVFDG+G A W  N+CH    CKKL
Sbjct: 81  IRIALLGTVKADGNANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKL 140

Query: 138 PMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSE 197
           P++++F+F+T++ ++ ITSLD+K+FHINV+  KN+TF+ +KI AP  SPNTDGIH+G S+
Sbjct: 141 PISIRFDFVTDAKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSD 200

Query: 198 DVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKC 257
            + I+N+ I+TGDDC+SVG   + + +  VTCGPGHGISIGSLG+Y  E+ V G+ +  C
Sbjct: 201 GIKIINSFISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINC 260

Query: 258 KLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTI 317
            L  T NG+RIK+WP +  +  ASD+HFE+I + NV+NPI+IDQEYCPWNQCN++ PSTI
Sbjct: 261 TLQETDNGLRIKTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTI 320

Query: 318 KISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEGPIVSECANVKPTV 377
           K++ +SFK IRGTS    AVKL+CSK +PC++VE+ D+++ Y+G +GP   +C+NV P +
Sbjct: 321 KLANISFKKIRGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKL 380

Query: 378 SGKQNPPICAKPA--PPE 394
            G Q P  C+ P   PP+
Sbjct: 381 VGTQIPKACSSPVTKPPK 395

BLAST of CmoCh05G008230 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 354.4 bits (908), Expect = 1.2e-97
Identity = 182/375 (48.53%), Postives = 242/375 (64.53%), Query Frame = 0

Query: 24  DITKFGAKPN--ADVTQALADAWKEACASATPSKVLVPKGTYQLEESRLKGPCKSPIELL 83
           D+   GAK +   D + A A AWKEACA+   S + VPKG Y +E    KGPCK P+ L 
Sbjct: 72  DVKASGAKGDGKTDDSAAFAAAWKEACAAG--STITVPKGEYLVESLEFKGPCKGPVTLE 131

Query: 84  VEGTLQAS---PTDTEGDGLLVMEYVDHLTLAGT-GVFDGQGKAGWEKNDCHVKKECKKL 143
           + G  +A     T     G +  E +   TL G   +FDGQG   W+ NDC    +C  L
Sbjct: 132 LNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSL 191

Query: 144 PMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPEHSPNTDGIHLGDSE 203
           P+N++F  +TNS +  ITS +SK FH+N+L  KN+T   + I AP  S NTDGIH+G S 
Sbjct: 192 PINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSN 251

Query: 204 DVYILNTVIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKC 263
            V ++   I TGDDC+S+G     + + +V CGPGHGISIGSLG+Y  E+ V GVTV+KC
Sbjct: 252 GVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKC 311

Query: 264 KLIGTTNGVRIKSWPDSVVSYPASDMHFEDIEMDNVANPIIIDQEYCPWNQCNRKVPSTI 323
            +  T NGVRIK+WP S     AS++ FEDI MDNV+ P++IDQEYCP+  C   VPS +
Sbjct: 312 LIKNTDNGVRIKTWPGSPPGI-ASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKV 371

Query: 324 KISKVSFKNIRGTSSTPVAVKLVCSKSHPCEDVEIADIDLTYSGKEGPIVSECANVKPTV 383
           K+S V+ KNI+GTS+T VAVKL+CSK  PC ++ ++DI+L ++GKEGP VS C+N+KP +
Sbjct: 372 KLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPIL 431

Query: 384 SGKQNPPICAKPAPP 393
           SGK  P  C + A P
Sbjct: 432 SGKLVPAACTEVAKP 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P245481.0e-12059.05Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
P353373.2e-11951.79Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1[more]
Q403121.0e-11755.01Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Q059673.9e-11753.09Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
Q397864.5e-11350.87Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EYY12.7e-230100.00exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439949 PE=3 SV=... [more]
A0A6J1I9Y34.8e-22798.25exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472545 PE=3 SV=1[more]
A0A6J1G4549.4e-22396.24exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111450616 PE=3 SV=... [more]
A0A6J1ENZ54.7e-22296.24exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111436229 PE=3 SV=... [more]
A0A6J1I8V23.0e-22196.01exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472559 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G07820.15.6e-11953.26Pectin lyase-like superfamily protein [more]
AT3G07840.11.1e-11750.88Pectin lyase-like superfamily protein [more]
AT3G07830.13.9e-11249.60Pectin lyase-like superfamily protein [more]
AT5G48140.14.3e-11148.68Pectin lyase-like superfamily protein [more]
AT3G14040.11.2e-9748.53Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 280..301
e-value: 6200.0
score: 1.6
coord: 249..270
e-value: 19.0
score: 14.2
coord: 219..239
e-value: 4000.0
score: 2.9
coord: 196..217
e-value: 860.0
score: 7.7
coord: 169..195
e-value: 970.0
score: 7.3
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 48..376
e-value: 1.1E-103
score: 346.7
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 226..239
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 10..395
e-value: 8.2E-106
score: 356.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 376..399
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 10..390
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 10..390
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 11..388

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G008230.1CmoCh05G008230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity