CmoCh05G006810 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh05G006810
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	Cmo_Chr05: 3455838 .. 3456416 (+)
RNA-Seq Expression	CmoCh05G006810
Synteny	CmoCh05G006810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAATACCAACTCAAAATGAAGGCTTTAGCTGATAAGCTAATAAGCTTAATCTTCAAGTTTCTTGGTATCTCCGATGAAGAGATGGTGAAGAAGTTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCATGCCCTGAACCAAGCAAAGTCATTGGCCTCGCGGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAGAGAGGGGTTACAGATCTTGAACGAGAAAGATGGCTGGCTTCCGCTGGCTCCGAGGAGCGATGCTCTCATTATTAACGTCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTCATAGCGTTCCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCAGGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACTCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCATCATCAAGGCTAAAGCCTCGGGGAAGGGAATTGCTGCCCTAACAATATGA

mRNA sequence

ATGGAAGAATACCAACTCAAAATGAAGGCTTTAGCTGATAAGCTAATAAGCTTAATCTTCAAGTTTCTTGGTATCTCCGATGAAGAGATGGTGAAGAAGTTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCATGCCCTGAACCAAGCAAAGTCATTGGCCTCGCGGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAGAGAGGGGTTACAGATCTTGAACGAGAAAGATGGCTGGCTTCCGCTGGCTCCGAGGAGCGATGCTCTCATTATTAACGTCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTCATAGCGTTCCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCAGGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACTCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCATCATCAAGGCTAAAGCCTCGGGGAAGGGAATTGCTGCCCTAACAATATGA

Coding sequence (CDS)

ATGGAAGAATACCAACTCAAAATGAAGGCTTTAGCTGATAAGCTAATAAGCTTAATCTTCAAGTTTCTTGGTATCTCCGATGAAGAGATGGTGAAGAAGTTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCATGCCCTGAACCAAGCAAAGTCATTGGCCTCGCGGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAGAGAGGGGTTACAGATCTTGAACGAGAAAGATGGCTGGCTTCCGCTGGCTCCGAGGAGCGATGCTCTCATTATTAACGTCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTCATAGCGTTCCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCAGGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACTCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCATCATCAAGGCTAAAGCCTCGGGGAAGGGAATTGCTGCCCTAACAATATGA

Protein sequence

MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKASGKGIAALTI

Homology

[Close]

BLAST of CmoCh05G006810 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 136.3 bits (342), Expect = 3.5e-31
Identity = 70/190 (36.84%), Postives = 114/190 (60.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           +EEY+  MK LA KL+ L    LG+S+E++  + + +         AL+LN +P CPEP 
Sbjct: 167 VEEYEEHMKKLASKLMWLALNSLGVSEEDI--EWASLSSDLNWAQAALQLNHYPVCPEPD 226

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           + +GLAAHTD++L T+L+Q    GLQ+  +  GW+ + P   +L++NVGD   I+SNG F
Sbjct: 227 RAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLF 286

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            SV HR  + +T +  +S+A+ + P   + ++P  K L   +++P+Y+ V  KEY   KA
Sbjct: 287 KSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK-LVSPVESPLYQSVTWKEYLRTKA 346

Query: 181 KASGKGIAAL 191
               K ++ +
Sbjct: 347 THFNKALSMI 352

[Close]

BLAST of CmoCh05G006810 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.3e-30
Identity = 71/195 (36.41%), Postives = 115/195 (58.97%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGIS--DEEMVKKLSYIDPATGKPHLALRLNSFPPCPE 60
           ++EY  +M+ LA +L+  I   LG++  D E   KL       G+   A+RLN +P CPE
Sbjct: 160 IQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRG--AIRLNHYPVCPE 219

Query: 61  PSKVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNG 120
           P + +GLAAHTD+++ T+LHQ+   GLQ+  E+ GW+ + P    L++N+GD   I+SNG
Sbjct: 220 PERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNG 279

Query: 121 RFHSVPHRVMIRETEKTTMSMAYFF-HPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFI 180
           +  SV HR  +  T ++ +S+AY +  P G + +AP  K L+   +  +Y+ +  KEY  
Sbjct: 280 KIPSVVHRAKVNHT-RSRISIAYLWGGPAGDVQIAPISK-LTGPAEPSLYRSITWKEYLQ 339

Query: 181 IKAKASGKGIAALTI 193
           IK +   K + A+ +
Sbjct: 340 IKYEVFDKAMDAIRV 350

[Close]

BLAST of CmoCh05G006810 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.1e-30
Identity = 70/190 (36.84%), Postives = 109/190 (57.37%), Query Frame = 0


Query: 3   EYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKV 62
           +Y   MK LA  L+ L+   LGI+ E++  K +       K   AL+LNS+P CP+P   
Sbjct: 168 QYDETMKKLAGTLMCLMLDSLGITKEDI--KWAGSKAQFEKACAALQLNSYPSCPDPDHA 227

Query: 63  IGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRFHS 122
           +GLA HTD++  T+L Q    GLQ+  E  GW+ + P    L++NVGD   I+SNG + S
Sbjct: 228 MGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPS 287

Query: 123 VPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKA 182
           V HRV++  T +   S+AY + PP ++ + P+ K +  T + P+Y+ V   EY   KAK 
Sbjct: 288 VLHRVLVNRT-RQRFSVAYLYGPPSNVEICPHAKLIGPT-KPPLYRSVTWNEYLGTKAKH 347

Query: 183 SGKGIAALTI 193
             K ++++ +
Sbjct: 348 FNKALSSVRL 353

[Close]

BLAST of CmoCh05G006810 vs. ExPASy Swiss-Prot
Match: Q3I409 (Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum OX=4565 GN=GA3ox2-3 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.7e-30
Identity = 74/196 (37.76%), Postives = 112/196 (57.14%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMV-----KKLSYIDPATGKPHLALRLNSFPP 60
           MEE+  +M+ALADKL+ L    LG++ E++      +K++    AT      + LN +P 
Sbjct: 163 MEEFHREMRALADKLLELFLVALGLTGEQVAAVESEQKIAETMTAT------MHLNWYPK 222

Query: 61  CPEPSKVIGLAAHTDTSLFTMLHQARREGLQILNE-KDGWLPLAPRSDALIINVGDFLQI 120
           CP+P + +GL AHTD+  FT + Q+   GLQ+     D W+ +     A+++NVGD  QI
Sbjct: 223 CPDPKRALGLIAHTDSGFFTFVLQSLVPGLQLFRHGPDRWVTVPAVPGAMVVNVGDLFQI 282

Query: 121 ISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKE 180
           ++NGRFHSV HR ++   E   +S+ YF  PP H+ VAP  + L+ T     Y+ V   E
Sbjct: 283 LTNGRFHSVYHRAVVNR-ESDRISLGYFLGPPAHVKVAPLREALAGT--PAAYRAVTWPE 342

Query: 181 YFIIKAKASGKGIAAL 191
           Y  ++ KA   G +AL
Sbjct: 343 YMGVRKKAFTTGASAL 349

[Close]

BLAST of CmoCh05G006810 vs. ExPASy Swiss-Prot
Match: Q3I410 (Gibberellin 3-beta-dioxygenase 2-2 OS=Triticum aestivum OX=4565 GN=GA3ox2-2 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.5e-29
Identity = 73/196 (37.24%), Postives = 112/196 (57.14%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMV-----KKLSYIDPATGKPHLALRLNSFPP 60
           MEE+  +M+ALADKL+ L    LG++ E++      +K++    AT      + LN +P 
Sbjct: 163 MEEFHREMRALADKLLELFLVALGLTGEQVAAVESEQKIAETMTAT------MHLNWYPK 222

Query: 61  CPEPSKVIGLAAHTDTSLFTMLHQARREGLQILNE-KDGWLPLAPRSDALIINVGDFLQI 120
           CP+P + +GL AHTD+  FT + Q+   GLQ+     D W+ +     A+++NVGD  QI
Sbjct: 223 CPDPKRALGLIAHTDSGFFTFVLQSLVPGLQLFRHGPDRWVTVPAVPGAMVVNVGDLFQI 282

Query: 121 ISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKE 180
           ++NGRFHSV HR ++   +   +S+ YF  PP H+ VAP  + L+ T     Y+ V   E
Sbjct: 283 LTNGRFHSVYHRAVVNR-DSDRISLGYFLGPPAHVKVAPLREALAGT--PAAYRAVTWPE 342

Query: 181 YFIIKAKASGKGIAAL 191
           Y  ++ KA   G +AL
Sbjct: 343 YMGVRKKAFTTGASAL 349

[Close]

BLAST of CmoCh05G006810 vs. ExPASy TrEMBL
Match: A0A6J1ESR0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436163 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.3e-105
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF
Sbjct: 215 KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. ExPASy TrEMBL
Match: A0A6J1ESQ5 (gibberellin 3-beta-dioxygenase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436160 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.9e-105
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIIN+GDFLQIISNGRF
Sbjct: 215 KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINIGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. ExPASy TrEMBL
Match: A0A6J1ENP5 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436161 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.9e-105
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIIN+GDFLQIISNGRF
Sbjct: 215 KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINIGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. ExPASy TrEMBL
Match: A0A6J1EPC3 (gibberellin 3-beta-dioxygenase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436160 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.9e-105
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIIN+GDFLQIISNGRF
Sbjct: 215 KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINIGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. ExPASy TrEMBL
Match: A0A6J1ENA3 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436162 PE=4 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.9e-105
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIIN+GDFLQIISNGRF
Sbjct: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINIGDFLQIISNGRF 120

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 181 KASGKGIAALTI 192

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: XP_022929638.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 391.3 bits (1004), Expect = 4.6e-105
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF
Sbjct: 215 KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: XP_022929634.1 (gibberellin 3-beta-dioxygenase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 391.0 bits (1003), Expect = 6.1e-105
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIIN+GDFLQIISNGRF
Sbjct: 215 KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINIGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: XP_022929637.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 391.0 bits (1003), Expect = 6.1e-105
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIIN+GDFLQIISNGRF
Sbjct: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINIGDFLQIISNGRF 120

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 181 KASGKGIAALTI 192

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: XP_022929636.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 391.0 bits (1003), Expect = 6.1e-105
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIIN+GDFLQIISNGRF
Sbjct: 215 KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINIGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: XP_022929633.1 (gibberellin 3-beta-dioxygenase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 391.0 bits (1003), Expect = 6.1e-105
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIIN+GDFLQIISNGRF
Sbjct: 215 KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINIGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-32
Identity = 70/190 (36.84%), Postives = 114/190 (60.00%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           +EEY+  MK LA KL+ L    LG+S+E++  + + +         AL+LN +P CPEP 
Sbjct: 167 VEEYEEHMKKLASKLMWLALNSLGVSEEDI--EWASLSSDLNWAQAALQLNHYPVCPEPD 226

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           + +GLAAHTD++L T+L+Q    GLQ+  +  GW+ + P   +L++NVGD   I+SNG F
Sbjct: 227 RAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLF 286

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            SV HR  + +T +  +S+A+ + P   + ++P  K L   +++P+Y+ V  KEY   KA
Sbjct: 287 KSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK-LVSPVESPLYQSVTWKEYLRTKA 346

Query: 181 KASGKGIAAL 191
               K ++ +
Sbjct: 347 THFNKALSMI 352

[Close]

BLAST of CmoCh05G006810 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 133.7 bits (335), Expect = 1.6e-31
Identity = 71/195 (36.41%), Postives = 115/195 (58.97%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGIS--DEEMVKKLSYIDPATGKPHLALRLNSFPPCPE 60
           ++EY  +M+ LA +L+  I   LG++  D E   KL       G+   A+RLN +P CPE
Sbjct: 160 IQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRG--AIRLNHYPVCPE 219

Query: 61  PSKVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNG 120
           P + +GLAAHTD+++ T+LHQ+   GLQ+  E+ GW+ + P    L++N+GD   I+SNG
Sbjct: 220 PERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNG 279

Query: 121 RFHSVPHRVMIRETEKTTMSMAYFF-HPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFI 180
           +  SV HR  +  T ++ +S+AY +  P G + +AP  K L+   +  +Y+ +  KEY  
Sbjct: 280 KIPSVVHRAKVNHT-RSRISIAYLWGGPAGDVQIAPISK-LTGPAEPSLYRSITWKEYLQ 339

Query: 181 IKAKASGKGIAALTI 193
           IK +   K + A+ +
Sbjct: 340 IKYEVFDKAMDAIRV 350

[Close]

BLAST of CmoCh05G006810 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-29
Identity = 67/182 (36.81%), Postives = 105/182 (57.69%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATG--KPHLALRLNSFPPCPE 60
           +EEY+  M+ LA KL+      LG+ +    K + +  P +        ++LN +P CPE
Sbjct: 160 IEEYEEHMQKLAAKLMWFALGSLGVEE----KDIQWAGPNSDFQGTQAVIQLNHYPKCPE 219

Query: 61  PSKVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNG 120
           P + +GLAAHTD++L T+L+Q    GLQ+  +  GW+   P   +L++NVGD L I++NG
Sbjct: 220 PDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNG 279

Query: 121 RFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFII 180
            F SV HR  +    ++  SMAY + PP  + ++P  K L + LQ+P+Y  +  K+Y   
Sbjct: 280 IFPSVLHRARVNHV-RSRFSMAYLWGPPSDIMISPLPK-LVDPLQSPLYPSLTWKQYLAT 335

[Close]

BLAST of CmoCh05G006810 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 117.5 bits (293), Expect = 1.2e-26
Identity = 71/195 (36.41%), Postives = 106/195 (54.36%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDP-----ATGKPHLALRLNSFPP 60
           MEEYQ  M  L+ +LIS++   LG++ E+    L ++ P      T      L+LNS+P 
Sbjct: 158 MEEYQKAMDDLSHRLISMLMGSLGLTHED----LGWLVPDKTGSGTDSIQSFLQLNSYPV 217

Query: 61  CPEPSKVIGLAAHTDTSLFTMLHQARREGLQI---LNEKDGWLPLAPRSDALIINVGDFL 120
           CP+P   +GLA HTD+SL T+L+Q    GL+I     E   W+ + P   +L++ +GD  
Sbjct: 218 CPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLS 277

Query: 121 QIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNV 180
            IISNG+F S  HR ++ +T    +S AYF  PP +L + P     S+    PIY+ +  
Sbjct: 278 HIISNGQFRSTMHRAVVNKTHH-RVSAAYFAGPPKNLQIGPL---TSDKNHPPIYRRLIW 337

Query: 181 KEYFIIKAKASGKGI 188
           +EY   KA    K +
Sbjct: 338 EEYLAAKATHFNKAL 344

[Close]

BLAST of CmoCh05G006810 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 5.1e-25
Identity = 66/189 (34.92%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           +E Y  ++K++A  L + +   L I  EEM      +         ++++N +PPCP+P 
Sbjct: 171 LETYSSEVKSIAKILFAKMASVLEIKHEEMEDLFDDV-------WQSIKINYYPPCPQPD 230

Query: 61  KVIGLAAHTDTSLFTMLHQARR-EGLQILNEKDG-WLPLAPRSDALIINVGDFLQIISNG 120
           +V+GL  H+D +  T+L Q  + EGLQI  +KDG W+ + P  DAL++NVG+ L+II+NG
Sbjct: 231 QVMGLTQHSDAAGLTILLQVNQVEGLQI--KKDGKWVVVKPLRDALVVNVGEILEIITNG 290

Query: 121 RFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYF-- 180
           R+ S+ HR ++  +EK  +S+A  FH PG   +    K L +  +  ++K ++ +EYF  
Sbjct: 291 RYRSIEHRAVV-NSEKERLSVA-MFHSPGKETIIRPAKSLVDRQKQCLFKSMSTQEYFDA 348

Query: 181 IIKAKASGK 186
               K +GK
Sbjct: 351 FFTQKLNGK 348

The following BLAST results are available for this feature:

BLAST of CmoCh05G006810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39103	3.5e-31	36.84	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9C971	2.3e-30	36.41	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
O24648	5.1e-30	36.84	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q3I409	6.7e-30	37.76	Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum OX=4565 GN=GA3ox2-3 PE=2...	[more]
Q3I410	1.5e-29	37.24	Gibberellin 3-beta-dioxygenase 2-2 OS=Triticum aestivum OX=4565 GN=GA3ox2-2 PE=2...	[more]

BLAST of CmoCh05G006810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ESR0	2.3e-105	100.00	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436...	[more]
A0A6J1ESQ5	2.9e-105	99.48	gibberellin 3-beta-dioxygenase 1-like isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1ENP5	2.9e-105	99.48	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436...	[more]
A0A6J1EPC3	2.9e-105	99.48	gibberellin 3-beta-dioxygenase 1-like isoform X2 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1ENA3	2.9e-105	99.48	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436...	[more]

BLAST of CmoCh05G006810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022929638.1	4.6e-105	100.00	gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata]	[more]
XP_022929634.1	6.1e-105	99.48	gibberellin 3-beta-dioxygenase 1-like isoform X2 [Cucurbita moschata]	[more]
XP_022929637.1	6.1e-105	99.48	gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata]	[more]
XP_022929636.1	6.1e-105	99.48	gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata]	[more]
XP_022929633.1	6.1e-105	99.48	gibberellin 3-beta-dioxygenase 1-like isoform X1 [Cucurbita moschata]	[more]

BLAST of CmoCh05G006810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15550.1	2.5e-32	36.84	gibberellin 3-oxidase 1	[more]
AT1G80330.1	1.6e-31	36.41	gibberellin 3-oxidase 4	[more]
AT1G80340.1	1.2e-29	36.81	gibberellin 3-oxidase 2	[more]
AT4G21690.1	1.2e-26	36.41	gibberellin 3-oxidase 3	[more]
AT1G78550.1	5.1e-25	34.92	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 46..145 e-value: 4.1E-24 score: 85.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..177 e-value: 1.8E-45 score: 157.9
None	No IPR available	PANTHER	PTHR47991:SF77	GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKE	coord: 1..183
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 1..183
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..183
IPR001261	ArgE/DapE/ACY1/CPG2/YscS, conserved site	PROSITE	PS00758	ARGE_DAPE_CPG2_1	coord: 63..72
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 43..145 score: 10.181151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh05G006810.1	CmoCh05G006810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding