CmoCh05G003500 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G003500
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMajor facilitator superfamily protein
LocationCmo_Chr05: 1571318 .. 1573729 (-)
RNA-Seq ExpressionCmoCh05G003500
SyntenyCmoCh05G003500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAACTTCGTCAATGGGCGATGGATCTCTACGAAGCTCCAAAAATGGCGAAACGACCAAATTCCGATCCATCCGACAGCAACAAACTTCTCTCAATCATGGCACAGCCATTCCGGTGGTTTAAAATGCTCTCATCGGAGCTAAACCCGAGTTTCATCGCCGGCGTGGTGCTCGTCTACGGCTTAAGCCACGGCTTCTCCGGCTCCTATTTCAAAGTCGTCTCCGATTACTATTGGAAAGACGTGCAGAAGCTACAACCGTCGGTGGTTCAGATCTACATCGGGATTTACACGATTCCATGGGTTATGAAGCCGATTTGGGGGCTATTGACGGACGCGTTTCCGGTGAGAGGGTACCTACGGAGGCCGTATTTCGTGATTGCGGGGGTGGTTGGGACGGCGTCGGCGGTGGCGGTGGCGATGAAGGAAGGATTGGGGATTTTGGGAGCTCTAGGGCTTTTGATTGGGATTTCGGCGGCGATGGCGATTGCGGATGTGACGATCGATGCTTGCATTGTGCGGAGTAGCATTGAAGTGCGGTGGTTGGCGCAGGATTTGCAGAGCCTCTGTGGGGCTTGCTCTTCCATTGGCCATTTGATTGGGTATTCCACCAGTGGCGTTTTTGTTCATCTCCTCGGCGCTCAGGTCTGTAAAAACTAAAAAATAATATGAAAATACAAGAAAATAAAAATGTGAAATGACGGTTTTCTCCTTGGTCCTTATGACTTTTTATTTTGCAGTTTAGTCCTTGAATTAGTTACTAATTACTAAGAAGCTAATATGCAGGAAGCACTTGGGATCCTGGCAATTCCCCCAGCCATGATAATCGTGTTGGGCTTCTTCATACATGAGCCACGCTCCTCCACCCTCCAATCTTATGGGAAGCCCAAGGTCTTTCTCTCCCTAAAATACCCTCAACTTTTTATTTATTTATTTAATATATATATATATATATATATATATATATATATTAGAGTTATCTTGTTTTTCAAAACTATATTTAAAATAAAAACGACTGAATTTTTGTACACGCGCCTTTGAAACGACCCAATTAAGATTTTGATTTTAAGTACGTATGTACACTTTTATAGGCTATATTTCTTCGAATTTTGGGTTGAAATCATGAAATTTGAACATTCACGCAACAATATTGAAAAGTATCGATTGTTACTCAATTTTTCACGCGTGTTTCAAATGACCAAACTAGGGTTTCTAAATAAAAGTTTCGATTCTAAGTCCGTACCTACATTGTTTTCGCTTTTTTTCTTCGAAATTTGAGTTGAAATACCCGAATATGAACATTCCTCACAATAGTGTTTTAAAATATCGATGGTTTTTTTTACGCGCCTATTTAGAACGACCTAATTGAGATTTTGATTTTAAGCATGTATGTACACTGTTGTAGATTATATTTCTTCCAAATTTGAGTTTATTAAAATTTAGAACGAAATTCTATGTCTCATTTTTTATTTAAAAATTATAATGTATTTTTATGTAATTTTGAAATATTTATAAGAATTTAAAATAATGTAAAATAAATTTAATTCATAATATATATGCAGGGAGAGTCTTTGAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCGCACGTGTGGAAGCCTGCGCTTTTCATGTTCCTCTGTCTCTCTCTCAGTATAAGTACTCACGAGGGCCAATTCTATTGGTACACGGACAAGAAAGCCGGCCCAGCCTTCTCTCAGGTCGTCGACATGTCGTATTGAGTACAAACGTCATGTCGATTTCTTTAACAATCTCTTGCTTTATACTCGCAGGAATCGGTGGGGCTCATTTACGCAGTCGGCTCAGCGGCGTCTCTAATCGGAGTACTAATCTACCACAAAACGCTCAGAGACGTAAAATTCCGACGAATCCTCTTCTTCGCTCAGCTTTTCTACGGCGTCGCCGGACTTCTCGATGTCGTTTTCATCCTCCGGTGGAATTTAGTCCTAAAAATTCCGGATGAGTTATTCGTCGTTTTGGAAGAGTGCGTTTCTCGAATCGTGTCGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTCTGTTCCGTAGGGATCGAAGGAACGTTCTTCGCATTATTGGCGTGTATTGATAGCCTTGGCATGCTGTGCTCGAAATGGAGCGGCGGATTGGTGCTCCATGCTTTTGGAGTCACGAGGAGTGATTTTAGGAACCTTTGGTTGGTGGTTTTGCTTCGGAGTCTGCTGAGATTTGTGGTTCTGGCGTTCGTTTTTCTTGTTCCTGATGCAAGTCAGTCGGACATTTTGGTTCCGTCCGATCAGACGGTGAGTAGAAAAAGTTCGACGACTGTGGAGGAAGATGGCGATAGCATTCCTCTCGTCTCCATGAAAGGAGAGGGCCGAGGTTAG

mRNA sequence

TTAACTTCGTCAATGGGCGATGGATCTCTACGAAGCTCCAAAAATGGCGAAACGACCAAATTCCGATCCATCCGACAGCAACAAACTTCTCTCAATCATGGCACAGCCATTCCGGTGGTTTAAAATGCTCTCATCGGAGCTAAACCCGAGTTTCATCGCCGGCGTGGTGCTCGTCTACGGCTTAAGCCACGGCTTCTCCGGCTCCTATTTCAAAGTCGTCTCCGATTACTATTGGAAAGACGTGCAGAAGCTACAACCGTCGGTGGTTCAGATCTACATCGGGATTTACACGATTCCATGGGTTATGAAGCCGATTTGGGGGCTATTGACGGACGCGTTTCCGGTGAGAGGGTACCTACGGAGGCCGTATTTCGTGATTGCGGGGGTGGTTGGGACGGCGTCGGCGGTGGCGGTGGCGATGAAGGAAGGATTGGGGATTTTGGGAGCTCTAGGGCTTTTGATTGGGATTTCGGCGGCGATGGCGATTGCGGATGTGACGATCGATGCTTGCATTGTGCGGAGTAGCATTGAAGTGCGGTGGTTGGCGCAGGATTTGCAGAGCCTCTGTGGGGCTTGCTCTTCCATTGGCCATTTGATTGGGTATTCCACCAGTGGCGTTTTTGTTCATCTCCTCGGCGCTCAGGAAGCACTTGGGATCCTGGCAATTCCCCCAGCCATGATAATCGTGTTGGGCTTCTTCATACATGAGCCACGCTCCTCCACCCTCCAATCTTATGGGAAGCCCAAGGGAGAGTCTTTGAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCGCACGTGTGGAAGCCTGCGCTTTTCATGTTCCTCTGTCTCTCTCTCAGTATAAGTACTCACGAGGGCCAATTCTATTGGTACACGGACAAGAAAGCCGGCCCAGCCTTCTCTCAGGAATCGGTGGGGCTCATTTACGCAGTCGGCTCAGCGGCGTCTCTAATCGGAGTACTAATCTACCACAAAACGCTCAGAGACGTAAAATTCCGACGAATCCTCTTCTTCGCTCAGCTTTTCTACGGCGTCGCCGGACTTCTCGATGTCGTTTTCATCCTCCGGTGGAATTTAGTCCTAAAAATTCCGGATGAGTTATTCGTCGTTTTGGAAGAGTGCGTTTCTCGAATCGTGTCGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTCTGTTCCGTAGGGATCGAAGGAACGTTCTTCGCATTATTGGCGTGTATTGATAGCCTTGGCATGCTGTGCTCGAAATGGAGCGGCGGATTGGTGCTCCATGCTTTTGGAGTCACGAGGAGTGATTTTAGGAACCTTTGGTTGGTGGTTTTGCTTCGGAGTCTGCTGAGATTTGTGGTTCTGGCGTTCGTTTTTCTTGTTCCTGATGCAAGTCAGTCGGACATTTTGGTTCCGTCCGATCAGACGGTGAGTAGAAAAAGTTCGACGACTGTGGAGGAAGATGGCGATAGCATTCCTCTCGTCTCCATGAAAGGAGAGGGCCGAGGTTAG

Coding sequence (CDS)

ATGGATCTCTACGAAGCTCCAAAAATGGCGAAACGACCAAATTCCGATCCATCCGACAGCAACAAACTTCTCTCAATCATGGCACAGCCATTCCGGTGGTTTAAAATGCTCTCATCGGAGCTAAACCCGAGTTTCATCGCCGGCGTGGTGCTCGTCTACGGCTTAAGCCACGGCTTCTCCGGCTCCTATTTCAAAGTCGTCTCCGATTACTATTGGAAAGACGTGCAGAAGCTACAACCGTCGGTGGTTCAGATCTACATCGGGATTTACACGATTCCATGGGTTATGAAGCCGATTTGGGGGCTATTGACGGACGCGTTTCCGGTGAGAGGGTACCTACGGAGGCCGTATTTCGTGATTGCGGGGGTGGTTGGGACGGCGTCGGCGGTGGCGGTGGCGATGAAGGAAGGATTGGGGATTTTGGGAGCTCTAGGGCTTTTGATTGGGATTTCGGCGGCGATGGCGATTGCGGATGTGACGATCGATGCTTGCATTGTGCGGAGTAGCATTGAAGTGCGGTGGTTGGCGCAGGATTTGCAGAGCCTCTGTGGGGCTTGCTCTTCCATTGGCCATTTGATTGGGTATTCCACCAGTGGCGTTTTTGTTCATCTCCTCGGCGCTCAGGAAGCACTTGGGATCCTGGCAATTCCCCCAGCCATGATAATCGTGTTGGGCTTCTTCATACATGAGCCACGCTCCTCCACCCTCCAATCTTATGGGAAGCCCAAGGGAGAGTCTTTGAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCGCACGTGTGGAAGCCTGCGCTTTTCATGTTCCTCTGTCTCTCTCTCAGTATAAGTACTCACGAGGGCCAATTCTATTGGTACACGGACAAGAAAGCCGGCCCAGCCTTCTCTCAGGAATCGGTGGGGCTCATTTACGCAGTCGGCTCAGCGGCGTCTCTAATCGGAGTACTAATCTACCACAAAACGCTCAGAGACGTAAAATTCCGACGAATCCTCTTCTTCGCTCAGCTTTTCTACGGCGTCGCCGGACTTCTCGATGTCGTTTTCATCCTCCGGTGGAATTTAGTCCTAAAAATTCCGGATGAGTTATTCGTCGTTTTGGAAGAGTGCGTTTCTCGAATCGTGTCGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTCTGTTCCGTAGGGATCGAAGGAACGTTCTTCGCATTATTGGCGTGTATTGATAGCCTTGGCATGCTGTGCTCGAAATGGAGCGGCGGATTGGTGCTCCATGCTTTTGGAGTCACGAGGAGTGATTTTAGGAACCTTTGGTTGGTGGTTTTGCTTCGGAGTCTGCTGAGATTTGTGGTTCTGGCGTTCGTTTTTCTTGTTCCTGATGCAAGTCAGTCGGACATTTTGGTTCCGTCCGATCAGACGGTGAGTAGAAAAAGTTCGACGACTGTGGAGGAAGATGGCGATAGCATTCCTCTCGTCTCCATGAAAGGAGAGGGCCGAGGTTAG

Protein sequence

MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVEEDGDSIPLVSMKGEGRG
Homology
BLAST of CmoCh05G003500 vs. ExPASy Swiss-Prot
Match: Q9LEV7 (Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana OX=3702 GN=At5g10820 PE=2 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.2e-168
Identity = 297/489 (60.74%), Postives = 373/489 (76.28%), Query Frame = 0

Query: 12  RPNSDPSD------SNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFK 71
           +P SD S+       N ++S++ QPF+W +MLSS LN SF+ GVVLVYG++ GFSGS FK
Sbjct: 18  KPISDHSEIKTKARRNAVVSVLIQPFQWLQMLSSRLNLSFVLGVVLVYGVNQGFSGSIFK 77

Query: 72  VVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVG 131
           VV+DYYWKDVQ++QPSVVQ+Y+G+Y IPWVM+PIWGL TD FP++GY R+PYFV++GV+G
Sbjct: 78  VVTDYYWKDVQQVQPSVVQLYMGLYYIPWVMRPIWGLFTDVFPIKGYKRKPYFVVSGVLG 137

Query: 132 TASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGA 191
             SA+A+ +   L    AL  L+G+SAAMAIADV IDACI  +SI +R LA D+QSLC  
Sbjct: 138 LVSAIAIVVLGKLPAALALSCLLGVSAAMAIADVVIDACIATNSINIRSLAPDIQSLCMV 197

Query: 192 CSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGE 251
           CSS G L+GY+TSGVFVH LG Q ALG+LA  PA I++LGFFI+E RSST+ +    K  
Sbjct: 198 CSSAGALVGYATSGVFVHQLGPQGALGVLAFSPATIVILGFFIYEKRSSTVPTQKTKK-- 257

Query: 252 SLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESV 311
               +GVA++  CK +KYP VWKP+L+MF+ L+L+ISTHEG FYWYTD  AGPAFSQE V
Sbjct: 258 DTDGLGVAVKGMCKTVKYPEVWKPSLYMFISLALNISTHEGHFYWYTDPTAGPAFSQEFV 317

Query: 312 GLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPD 371
           G+IYAVG+ AS+ GVLIYHK L+   FR ILFFAQL Y  +G+LD+VFI RWNL L IPD
Sbjct: 318 GIIYAVGALASMFGVLIYHKKLKGYSFRNILFFAQLLYVFSGMLDLVFIKRWNLTLGIPD 377

Query: 372 ELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLV 431
            LFV+ EE  ++++S+IRW PM+VL+TRLC +GIEGTFFA L CIDS G L SKW GG V
Sbjct: 378 SLFVITEESFTKMISKIRWIPMVVLSTRLCPLGIEGTFFAFLMCIDSFGQLASKWGGGFV 437

Query: 432 LHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVE 491
           LHAFGVTR DF NLWLV+L+R++LR V + FVFLVPD+   D LVPSD    ++S    +
Sbjct: 438 LHAFGVTRHDFGNLWLVILIRNILRLVTVCFVFLVPDSDHLDDLVPSDVLPKKQSE---D 497

Query: 492 EDGDSIPLV 495
           +D D I L+
Sbjct: 498 DDDDDIKLL 501

BLAST of CmoCh05G003500 vs. ExPASy Swiss-Prot
Match: Q9SQN2 (Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana OX=3702 GN=At1g79710 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.0e-117
Identity = 216/486 (44.44%), Postives = 316/486 (65.02%), Query Frame = 0

Query: 10  AKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSD 69
           ++R + D      L  ++ +P RW +ML  EL+ SF  GV++VYG+S G      KV + 
Sbjct: 14  SRRNHQDKKKEGVLDLVLKRPLRWLRMLIEELHWSFFFGVIIVYGVSQGLGKGLSKVSTQ 73

Query: 70  YYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASA 129
           YY+KD QK+QPS  QIY+G+  IPW++KP+WGLLTD  PV GY RRPYF++AG +   S 
Sbjct: 74  YYFKDEQKIQPSQAQIYVGLIQIPWIIKPLWGLLTDVVPVLGYRRRPYFILAGFLAMISM 133

Query: 130 VAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSI 189
           + + +   L +  AL  L+  SA +AIADVTIDAC+ + SI    LA D+QSLCG CSSI
Sbjct: 134 MVLWLHTNLHLALALSCLVAGSAGVAIADVTIDACVTQCSISHPTLAADMQSLCGLCSSI 193

Query: 190 GHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQ 249
           G L+G+S SGV VHL+G++   G+L +   +++V+G  + E  S +L    K   +    
Sbjct: 194 GSLVGFSLSGVLVHLVGSKGVYGLLGVTAGLLVVVGMVLKESPSRSLGR--KHVNDKFLD 253

Query: 250 VGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIY 309
            G AI    K  +Y  VW+P LFM L  ++S+  HEG FYWYTD K GP+FS+E+VG I 
Sbjct: 254 AGSAI---WKTFQYGEVWRPCLFMLLSAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGSIM 313

Query: 310 AVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFV 369
           + G+  SL+G+L+Y   L++  FR ++F+A     ++G LD++ +LR NL L +PD  F+
Sbjct: 314 SFGAIGSLVGILLYQNFLKNFPFRNVVFWALSLSVLSGFLDLILVLRINLKLGLPDYFFI 373

Query: 370 VLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAF 429
           V++E VS ++SRI+W P++VL+++LC  G+EGTFFALL  I+ +G L S W GG++LHA 
Sbjct: 374 VVDEFVSHMISRIKWLPLLVLSSKLCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHAL 433

Query: 430 GVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVEEDGD 489
            VTR+ F NLWLV+++RSLLR + +  VFL+P+   +  ++P++   SR S     E  +
Sbjct: 434 KVTRTQFDNLWLVIVIRSLLRVIPIGLVFLIPNVDSNSTILPAEMLTSRISEGVDTEKIE 493

Query: 490 SIPLVS 496
              L+S
Sbjct: 494 MTALIS 494

BLAST of CmoCh05G003500 vs. ExPASy Swiss-Prot
Match: Q5FV41 (Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g25050 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.1e-111
Identity = 204/453 (45.03%), Postives = 300/453 (66.23%), Query Frame = 0

Query: 25  SIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQ 84
           +++  P RW KMLSSEL+ SF+ GVV +YG++ G  GS  +V ++YY KDVQK+QPS  Q
Sbjct: 25  NVVCGPVRWLKMLSSELHWSFVFGVVSLYGINQGLGGSLGRVATEYYMKDVQKVQPSESQ 84

Query: 85  IYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGAL 144
               I  IPW++KP+WG+LTD  P+ G+ RRPYF++AGV+G  S + +++   L +  AL
Sbjct: 85  ALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFILAGVLGVVSLLFISLHSNLHLYLAL 144

Query: 145 GLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHL 204
             +   SAAMAIADVTIDAC   +SI+   LA D+QSLC   SSIG L+G+  SG+ VHL
Sbjct: 145 FWMTISSAAMAIADVTIDACTAYNSIKHPSLASDMQSLCSLSSSIGALLGFFMSGILVHL 204

Query: 205 LGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQVGVAIRDTCKA---- 264
           +G++   G+L  P A++ V+G    EP            G S KQV     D  KA    
Sbjct: 205 VGSKGVFGLLTFPFALVSVVGIVFSEPH---------VPGFSYKQVNQKFTDAGKAMWRT 264

Query: 265 IKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGV 324
           +K   VW+P+L+M++ L+L ++ HEG FYW+TD K GP F+QE+VG I ++GS  S++  
Sbjct: 265 MKCSDVWRPSLYMYISLTLGLNIHEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILAA 324

Query: 325 LIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFVVLEECVSRIVS 384
            +Y   L+D  FR +  + QL + ++G+LD++ + R NL   +PD LF+V++E VS+++ 
Sbjct: 325 TLYQLVLKDHPFRGLCLWTQLLFALSGMLDLILVFRLNLKFGLPDYLFIVVDEIVSQMIG 384

Query: 385 RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAFGVTRSDFRNLW 444
           R++W P++VL ++LC  GIEGTFFALL  ID+ G++ S W GG++LH   VTR++F NLW
Sbjct: 385 RLKWMPLLVLTSKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGIMLHVLKVTRTEFGNLW 444

Query: 445 LVVLLRSLLRFVVLAFVFLVPDASQSDILVPSD 474
           L VL+R+++R + L F+FLVP   Q+   +P +
Sbjct: 445 LAVLVRNVMRLLPLCFLFLVPKGDQNTFKLPDE 468

BLAST of CmoCh05G003500 vs. ExPASy Swiss-Prot
Match: F4KIL8 (Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana OX=3702 GN=At5g25040 PE=2 SV=2)

HSP 1 Score: 373.2 bits (957), Expect = 4.5e-102
Identity = 197/455 (43.30%), Postives = 295/455 (64.84%), Query Frame = 0

Query: 21  NKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQP 80
           + L S++  P RW KML+S+L+ S++  VV   G++ GF  S   V +DYY KDVQK+QP
Sbjct: 21  SNLRSVVCGPVRWLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQP 80

Query: 81  SVVQIYIGIYTIPWVM-KPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLG 140
           S  Q    I  I W++ KP++G+LTD  P+ G+ RRPYF++AGV+G  S + ++++  L 
Sbjct: 81  SQYQALSAITRISWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLH 140

Query: 141 ILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSG 200
           +  AL  +   SAAMAIADVTIDAC   +S +   LA D+QSLC    SIG L+G+  SG
Sbjct: 141 LYLALFWMTISSAAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSG 200

Query: 201 VFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQ-VGVAIRDTC 260
           + VHL+G++   G+L    A++ V+G    EPR           G S KQ    A++   
Sbjct: 201 ILVHLVGSKGVFGLLTFTFALVSVVGVLFSEPR---------VHGFSFKQNFTNAMKAMW 260

Query: 261 KAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLI 320
           + IK   VW+P+L+MF+  +L +   EG FYW+TD K GP F+QE+VG I ++GS  S++
Sbjct: 261 RTIKCSDVWQPSLYMFITRALGLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSIL 320

Query: 321 GVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFVVLEECVSRI 380
           GVL+Y+  L+D  FR++  + QL + ++G+ D++ +LR NL   +PD LF+V++  VS++
Sbjct: 321 GVLLYNLRLKDHPFRKLFLWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKM 380

Query: 381 VSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAFGVTRSDFRN 440
           + R+ W  + VLNT+LC  GIEGTFFALL  ID+ G++ S W GG +LH   VTR++F N
Sbjct: 381 IIRLTWMVIFVLNTKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKVTRTEFGN 440

Query: 441 LWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSD 474
           LWL VL+R+++R + + F+FLVP   Q+   +P++
Sbjct: 441 LWLAVLVRNVMRLLPICFLFLVPQGDQNTFKLPAE 466

BLAST of CmoCh05G003500 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 175.6 bits (444), Expect = 1.4e-42
Identity = 140/456 (30.70%), Postives = 231/456 (50.66%), Query Frame = 0

Query: 35  KMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPW 94
           K    EL+P  +A V +VY    G  G     VS +Y KD   L P+   +  G+ ++PW
Sbjct: 117 KFYGVELSPDNVA-VAMVY-FVQGVLGLARLAVS-FYLKDDLHLDPAETAVITGLSSLPW 176

Query: 95  VMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGALGLLIGISAAM 154
           ++KP++G ++D+ P+ GY RR Y V++G++G  S   +A         A  +L+G S ++
Sbjct: 177 LVKPLYGFISDSVPLFGYRRRSYLVLSGLLGAFSWSLMAGFVDSKYSAAFCILLG-SLSV 236

Query: 155 AIADVTIDACIV-RSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLLGAQEALGI 214
           A +DV +D+ +V R+  E + ++  LQSLC   S+ G ++    SG  V   G +   G+
Sbjct: 237 AFSDVVVDSMVVERARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGV 296

Query: 215 LAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQVGVAIRDTCK--------AIKYPH 274
            A+ P +   +   ++E R     S  K   E++  +      T K        AIK P+
Sbjct: 297 TALLPLITSAVAVLVNEQRVVRPASGQK---ENITLLSPGFLQTSKQNMIQLWGAIKQPN 356

Query: 275 VWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHK 334
           V+ P LF+FL  +   S  +   +++T  K G  F+ E +G +  V S ASL+GV +Y+ 
Sbjct: 357 VFLPTLFIFLWQATPHS--DSAMFYFTTNKLG--FTPEFLGRVKLVTSIASLLGVGLYNG 416

Query: 335 TLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFVVLEECVSRIVSRIRWT 394
            L+ V  R+I     +F    G+  V+ +  +N  L I DE F + +  +  ++++  + 
Sbjct: 417 FLKTVPLRKIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFM 476

Query: 395 PMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAFGVTRSDFRNLWLVVLL 454
           P++VL  RLC  G+E T FA L  I + G +     G  +  AFG+TR  F NL  +++L
Sbjct: 477 PVLVLAARLCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIIL 536

Query: 455 RSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSS 482
            +L   + L  + L+P  S  D L   D  V  KS+
Sbjct: 537 CNLSSLLPLPLLGLLPRDS-PDTLTKDDADVEMKSN 560

BLAST of CmoCh05G003500 vs. ExPASy TrEMBL
Match: A0A6J1HEZ6 (probable folate-biopterin transporter 6 OS=Cucurbita moschata OX=3662 GN=LOC111462436 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 3.9e-282
Identity = 502/502 (100.00%), Postives = 502/502 (100.00%), Query Frame = 0

Query: 1   MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFS 60
           MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFS
Sbjct: 1   MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFS 60

Query: 61  GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI 120
           GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI
Sbjct: 61  GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI 120

Query: 121 AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ 180
           AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ
Sbjct: 121 AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ 180

Query: 181 SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG 240
           SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG
Sbjct: 181 SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG 240

Query: 241 KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF 300
           KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF
Sbjct: 241 KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF 300

Query: 301 SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLV 360
           SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLV
Sbjct: 301 SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLV 360

Query: 361 LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW 420
           LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW
Sbjct: 361 LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW 420

Query: 421 SGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS 480
           SGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS
Sbjct: 421 SGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS 480

Query: 481 STTVEEDGDSIPLVSMKGEGRG 503
           STTVEEDGDSIPLVSMKGEGRG
Sbjct: 481 STTVEEDGDSIPLVSMKGEGRG 502

BLAST of CmoCh05G003500 vs. ExPASy TrEMBL
Match: A0A6J1K7V4 (probable folate-biopterin transporter 6 OS=Cucurbita maxima OX=3661 GN=LOC111492472 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.8e-272
Identity = 485/494 (98.18%), Postives = 488/494 (98.79%), Query Frame = 0

Query: 9   MAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS 68
           MAKRPNSDPSDSNKLLSIMAQPFRW KMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS
Sbjct: 1   MAKRPNSDPSDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS 60

Query: 69  DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS 128
           DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS
Sbjct: 61  DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS 120

Query: 129 AVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSS 188
           AVAVA+KEGLGILGALGLLIGIS AMAIADVT+DACIVRSSIEVRWLAQDLQSLCGACSS
Sbjct: 121 AVAVAVKEGLGILGALGLLIGISTAMAIADVTVDACIVRSSIEVRWLAQDLQSLCGACSS 180

Query: 189 IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK 248
           IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK
Sbjct: 181 IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK 240

Query: 249 QVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI 308
            VGVAIRDTCKAIK PHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI
Sbjct: 241 HVGVAIRDTCKAIKCPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI 300

Query: 309 YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELF 368
           YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNL LKIPDELF
Sbjct: 301 YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELF 360

Query: 369 VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA 428
           VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA
Sbjct: 361 VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA 420

Query: 429 FGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVEEDG 488
           FGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQT+SRKSSTTVEEDG
Sbjct: 421 FGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTMSRKSSTTVEEDG 480

Query: 489 DSIPLVSMKGEGRG 503
           DSIPLVSMKGE RG
Sbjct: 481 DSIPLVSMKGESRG 494

BLAST of CmoCh05G003500 vs. ExPASy TrEMBL
Match: A0A0A0LMJ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G382710 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 8.2e-240
Identity = 419/484 (86.57%), Postives = 456/484 (94.21%), Query Frame = 0

Query: 18  SDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQK 77
           +++NKL SIM QPFRW KMLS+EL+PSFIAGV+L+YGLSHGFSGSYFKVVSDYYWKDVQK
Sbjct: 64  TNNNKLFSIMLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQK 123

Query: 78  LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEG 137
           LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI+GVVG  +AV +A+K G
Sbjct: 124 LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGG 183

Query: 138 LGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYST 197
           LG+L ALGLLIGIS AMAIADVTIDACIVR+SIEV+WLAQDLQSLCGACSSIGHLIGYST
Sbjct: 184 LGVLEALGLLIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYST 243

Query: 198 SGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQVGVAIRDT 257
           SG FVHLLGAQEALGILAIPPA++++LGFFIHE  S TLQ  GK KG S+KQVGVAIRDT
Sbjct: 244 SGFFVHLLGAQEALGILAIPPALVVLLGFFIHETPSPTLQYNGKSKGGSIKQVGVAIRDT 303

Query: 258 CKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL 317
           CKAIKYPHVW+P+LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL
Sbjct: 304 CKAIKYPHVWRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL 363

Query: 318 IGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFVVLEECVSR 377
           IGVLIYHKTLRD+KFRRILFFAQLFYGVAG LDV+F+LRWNL L+IPDELFVV+EECV+R
Sbjct: 364 IGVLIYHKTLRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTR 423

Query: 378 IVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAFGVTRSDFR 437
           IV+RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLG LCSKWSGGLVLHAFGVTRSDFR
Sbjct: 424 IVTRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFR 483

Query: 438 NLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVEEDGDSIPLVSMK 497
           NLWLVVLLR++LRFVV+ FVFLVPDA+Q+DIL+P D T+ RKSS+   EDGD+IPLVSMK
Sbjct: 484 NLWLVVLLRTVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSS---EDGDNIPLVSMK 543

Query: 498 GEGR 502
            EGR
Sbjct: 544 SEGR 544

BLAST of CmoCh05G003500 vs. ExPASy TrEMBL
Match: A0A1S3BD55 (probable folate-biopterin transporter 6 OS=Cucumis melo OX=3656 GN=LOC103488353 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 8.2e-240
Identity = 423/499 (84.77%), Postives = 462/499 (92.59%), Query Frame = 0

Query: 9   MAKRPNSDPS-----DSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSY 68
           MA  PNS+ S     +++KL SIM QPFRW KMLS+EL+PSFIAGV+L+YGLSHGFSGSY
Sbjct: 1   MANEPNSNLSTTTTTNNSKLFSIMLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSY 60

Query: 69  FKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGV 128
           FKVVSDYYWKDVQKLQPSVVQIY GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI+GV
Sbjct: 61  FKVVSDYYWKDVQKLQPSVVQIYTGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGV 120

Query: 129 VGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLC 188
           VG  +AV VA+K GLG+L ALGLLIG+S AMAIADVTIDACIVR+SIEV+WLAQDLQSLC
Sbjct: 121 VGATAAVGVAVKGGLGVLEALGLLIGVSGAMAIADVTIDACIVRNSIEVQWLAQDLQSLC 180

Query: 189 GACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPK 248
           GACSSIGHLIGYS+SG+FVHLLGAQEALGILAIPPA+II+LGFFIHE  S TLQ  GK K
Sbjct: 181 GACSSIGHLIGYSSSGIFVHLLGAQEALGILAIPPALIILLGFFIHETPSPTLQYNGKSK 240

Query: 249 GESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQE 308
           G  + QVGVAIR+TCKA+KYPHVW+P+LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQE
Sbjct: 241 GGCINQVGVAIRETCKAVKYPHVWRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQE 300

Query: 309 SVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKI 368
           SVGLIYAVGSAASLIGVLIYHKTLRD+KFRRILFFAQLFYGVAG LDV+F+LRWNL LKI
Sbjct: 301 SVGLIYAVGSAASLIGVLIYHKTLRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALKI 360

Query: 369 PDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGG 428
           PDELFVV+EECV+RIV+RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLG LCSKWSGG
Sbjct: 361 PDELFVVIEECVTRIVTRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGG 420

Query: 429 LVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS-ST 488
           LVLHAFGVTRSDFRNLWLVVLLR++LRFVV+AFVFLVPDA+QSDIL+P D T+ RKS S 
Sbjct: 421 LVLHAFGVTRSDFRNLWLVVLLRTVLRFVVVAFVFLVPDANQSDILIPGDPTMVRKSNSR 480

Query: 489 TVEEDGDSIPLVSMKGEGR 502
           T +EDGD+IPLVSM+ EGR
Sbjct: 481 TTKEDGDNIPLVSMRSEGR 499

BLAST of CmoCh05G003500 vs. ExPASy TrEMBL
Match: A0A5A7V9P7 (Putative folate-biopterin transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001960 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.2e-236
Identity = 413/476 (86.76%), Postives = 448/476 (94.12%), Query Frame = 0

Query: 27  MAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 86
           M QPFRW KMLS+EL+PSFIAGV+L+YGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY
Sbjct: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60

Query: 87  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGALGL 146
            GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI+GVVG  +AV VA+K GLG+L ALGL
Sbjct: 61  TGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGVAVKGGLGVLEALGL 120

Query: 147 LIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLLG 206
           LIG+S AMAIADVTIDACIVR+SIEV+WLAQDLQSLCGACSSIGHLIGYS+SG+FVHLLG
Sbjct: 121 LIGVSGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSSSGIFVHLLG 180

Query: 207 AQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQVGVAIRDTCKAIKYPHV 266
           AQEALGILAIPPA+II+LGFFIHE  S TLQ  GK KG  + QVGVAIR+TCKA+KYPHV
Sbjct: 181 AQEALGILAIPPALIILLGFFIHETPSPTLQYNGKSKGGCINQVGVAIRETCKAVKYPHV 240

Query: 267 WKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 326
           W+P+LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT
Sbjct: 241 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 300

Query: 327 LRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFVVLEECVSRIVSRIRWTP 386
           LRD+KFRRILFFAQLFYGVAG LDV+F+LRWNL LKIPDELFVV+EECV+RIV+RIRWTP
Sbjct: 301 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALKIPDELFVVIEECVTRIVTRIRWTP 360

Query: 387 MMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 446
           MMVLNTRLCSVGIEGTFFALLACIDSLG LCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR
Sbjct: 361 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 420

Query: 447 SLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS-STTVEEDGDSIPLVSMKGEGR 502
           ++LRFVV+AFVFLVPDA+QSDIL+P D T+ RKS S T +EDGD+IPLVSM+ EGR
Sbjct: 421 TVLRFVVVAFVFLVPDANQSDILIPGDPTMVRKSNSRTTKEDGDNIPLVSMRSEGR 476

BLAST of CmoCh05G003500 vs. NCBI nr
Match: XP_022961764.1 (probable folate-biopterin transporter 6 [Cucurbita moschata])

HSP 1 Score: 979.9 bits (2532), Expect = 8.0e-282
Identity = 502/502 (100.00%), Postives = 502/502 (100.00%), Query Frame = 0

Query: 1   MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFS 60
           MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFS
Sbjct: 1   MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFS 60

Query: 61  GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI 120
           GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI
Sbjct: 61  GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI 120

Query: 121 AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ 180
           AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ
Sbjct: 121 AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ 180

Query: 181 SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG 240
           SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG
Sbjct: 181 SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG 240

Query: 241 KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF 300
           KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF
Sbjct: 241 KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF 300

Query: 301 SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLV 360
           SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLV
Sbjct: 301 SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLV 360

Query: 361 LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW 420
           LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW
Sbjct: 361 LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW 420

Query: 421 SGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS 480
           SGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS
Sbjct: 421 SGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS 480

Query: 481 STTVEEDGDSIPLVSMKGEGRG 503
           STTVEEDGDSIPLVSMKGEGRG
Sbjct: 481 STTVEEDGDSIPLVSMKGEGRG 502

BLAST of CmoCh05G003500 vs. NCBI nr
Match: XP_023545761.1 (probable folate-biopterin transporter 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972.6 bits (2513), Expect = 1.3e-279
Identity = 498/502 (99.20%), Postives = 499/502 (99.40%), Query Frame = 0

Query: 1   MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFS 60
           MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRW KMLSSELNPSFIAGVVLVYGLSHGFS
Sbjct: 1   MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFS 60

Query: 61  GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI 120
           GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI
Sbjct: 61  GSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI 120

Query: 121 AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ 180
           AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ
Sbjct: 121 AGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQ 180

Query: 181 SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG 240
           SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG
Sbjct: 181 SLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYG 240

Query: 241 KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF 300
           KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF
Sbjct: 241 KPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAF 300

Query: 301 SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLV 360
           SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNL 
Sbjct: 301 SQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLA 360

Query: 361 LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW 420
           LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW
Sbjct: 361 LKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKW 420

Query: 421 SGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKS 480
           SGGLVLHA GVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVP+DQTVSRKS
Sbjct: 421 SGGLVLHALGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKS 480

Query: 481 STTVEEDGDSIPLVSMKGEGRG 503
           STTVEEDGDSIPLVSMKGEGRG
Sbjct: 481 STTVEEDGDSIPLVSMKGEGRG 502

BLAST of CmoCh05G003500 vs. NCBI nr
Match: KAG6598542.1 (putative folate-biopterin transporter 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029475.1 putative folate-biopterin transporter 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 954.9 bits (2467), Expect = 2.8e-274
Identity = 490/494 (99.19%), Postives = 490/494 (99.19%), Query Frame = 0

Query: 9   MAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS 68
           MAKRPNSDPSDSNKLLSIMAQPFRW KMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS
Sbjct: 1   MAKRPNSDPSDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS 60

Query: 69  DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS 128
           DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS
Sbjct: 61  DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS 120

Query: 129 AVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSS 188
           AVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSS
Sbjct: 121 AVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSS 180

Query: 189 IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK 248
           IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK
Sbjct: 181 IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK 240

Query: 249 QVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI 308
           QVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI
Sbjct: 241 QVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI 300

Query: 309 YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELF 368
           YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNL LKIPDELF
Sbjct: 301 YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELF 360

Query: 369 VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA 428
           VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA
Sbjct: 361 VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA 420

Query: 429 FGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVEEDG 488
            GVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSST VEEDG
Sbjct: 421 LGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTIVEEDG 480

Query: 489 DSIPLVSMKGEGRG 503
           DSIPLVSMKGEGRG
Sbjct: 481 DSIPLVSMKGEGRG 494

BLAST of CmoCh05G003500 vs. NCBI nr
Match: XP_022997581.1 (probable folate-biopterin transporter 6 [Cucurbita maxima])

HSP 1 Score: 947.2 bits (2447), Expect = 5.7e-272
Identity = 485/494 (98.18%), Postives = 488/494 (98.79%), Query Frame = 0

Query: 9   MAKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS 68
           MAKRPNSDPSDSNKLLSIMAQPFRW KMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS
Sbjct: 1   MAKRPNSDPSDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVS 60

Query: 69  DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS 128
           DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS
Sbjct: 61  DYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTAS 120

Query: 129 AVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSS 188
           AVAVA+KEGLGILGALGLLIGIS AMAIADVT+DACIVRSSIEVRWLAQDLQSLCGACSS
Sbjct: 121 AVAVAVKEGLGILGALGLLIGISTAMAIADVTVDACIVRSSIEVRWLAQDLQSLCGACSS 180

Query: 189 IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK 248
           IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK
Sbjct: 181 IGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLK 240

Query: 249 QVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI 308
            VGVAIRDTCKAIK PHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI
Sbjct: 241 HVGVAIRDTCKAIKCPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLI 300

Query: 309 YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELF 368
           YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNL LKIPDELF
Sbjct: 301 YAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELF 360

Query: 369 VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA 428
           VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA
Sbjct: 361 VVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHA 420

Query: 429 FGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVEEDG 488
           FGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQT+SRKSSTTVEEDG
Sbjct: 421 FGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTMSRKSSTTVEEDG 480

Query: 489 DSIPLVSMKGEGRG 503
           DSIPLVSMKGE RG
Sbjct: 481 DSIPLVSMKGESRG 494

BLAST of CmoCh05G003500 vs. NCBI nr
Match: XP_038886156.1 (probable folate-biopterin transporter 6 [Benincasa hispida])

HSP 1 Score: 875.9 bits (2262), Expect = 1.6e-250
Identity = 446/512 (87.11%), Postives = 477/512 (93.16%), Query Frame = 0

Query: 1   MDLYEAPKMAKRPNSDPSDS--------NKLLSIMAQPFRWFKMLSSELNPSFIAGVVLV 60
           MDL EAP+MAK PNSDPS++        NK LSIM+QPFRW KMLSSELNPSFIAGV+LV
Sbjct: 1   MDLSEAPRMAKEPNSDPSNNNNNNSNNKNKFLSIMSQPFRWLKMLSSELNPSFIAGVILV 60

Query: 61  YGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGY 120
           YGLSHGFS SYFKVVSDYYWKDVQKLQPSVVQIYIGIY IPWVMKPIWGLLTDAFPVRGY
Sbjct: 61  YGLSHGFSSSYFKVVSDYYWKDVQKLQPSVVQIYIGIYAIPWVMKPIWGLLTDAFPVRGY 120

Query: 121 LRRPYFVIAGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEV 180
           LRRPYFVIAGVVG  +AVAVA+K GLG+L ALGLLIGISAAMAIADVTIDACIVR+SIEV
Sbjct: 121 LRRPYFVIAGVVGATAAVAVAVKGGLGVLEALGLLIGISAAMAIADVTIDACIVRNSIEV 180

Query: 181 RWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPR 240
           +WLAQDLQSLCGACSSIGHLIGYS SG FVHLLGAQEALGILAIPPAMII+LGFF+HE  
Sbjct: 181 QWLAQDLQSLCGACSSIGHLIGYSASGFFVHLLGAQEALGILAIPPAMIILLGFFMHETP 240

Query: 241 SSTLQSYGKPKGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYT 300
           S TLQ  GK K  S+KQVGVAIRDTCKAIKYPHVWKP+LFMFLCLSLSISTHEGQFYWYT
Sbjct: 241 SITLQYNGKSKAGSIKQVGVAIRDTCKAIKYPHVWKPSLFMFLCLSLSISTHEGQFYWYT 300

Query: 301 DKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVV 360
           DKKAGPAFSQESVGLIYA+GSAASLIGVLIYHKTLR++KFRRILFFAQ+FYGVAG LDV+
Sbjct: 301 DKKAGPAFSQESVGLIYAIGSAASLIGVLIYHKTLRNLKFRRILFFAQIFYGVAGFLDVI 360

Query: 361 FILRWNLVLKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDS 420
           F+LRWNL LKIPD+LFVVLEECV+RIV+RIRWTPMMVLNTRLCSVGIEGTFFALLACIDS
Sbjct: 361 FVLRWNLALKIPDQLFVVLEECVTRIVTRIRWTPMMVLNTRLCSVGIEGTFFALLACIDS 420

Query: 421 LGMLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPS 480
           LG LCSKWSGGLVLHAFGVTRSDFRNLW+VVLLR++LRFVV+AFVFLVPDA+QSDILVP+
Sbjct: 421 LGTLCSKWSGGLVLHAFGVTRSDFRNLWMVVLLRTVLRFVVVAFVFLVPDANQSDILVPA 480

Query: 481 DQTVSRK--SSTTVEEDGDSIPLVSMKGEGRG 503
           DQT+ RK  SST V+EDGDSIPLVSMKGEGRG
Sbjct: 481 DQTMIRKSFSSTNVKEDGDSIPLVSMKGEGRG 512

BLAST of CmoCh05G003500 vs. TAIR 10
Match: AT5G10820.1 (Major facilitator superfamily protein )

HSP 1 Score: 594.3 bits (1531), Expect = 8.8e-170
Identity = 297/489 (60.74%), Postives = 373/489 (76.28%), Query Frame = 0

Query: 12  RPNSDPSD------SNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFK 71
           +P SD S+       N ++S++ QPF+W +MLSS LN SF+ GVVLVYG++ GFSGS FK
Sbjct: 18  KPISDHSEIKTKARRNAVVSVLIQPFQWLQMLSSRLNLSFVLGVVLVYGVNQGFSGSIFK 77

Query: 72  VVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVG 131
           VV+DYYWKDVQ++QPSVVQ+Y+G+Y IPWVM+PIWGL TD FP++GY R+PYFV++GV+G
Sbjct: 78  VVTDYYWKDVQQVQPSVVQLYMGLYYIPWVMRPIWGLFTDVFPIKGYKRKPYFVVSGVLG 137

Query: 132 TASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGA 191
             SA+A+ +   L    AL  L+G+SAAMAIADV IDACI  +SI +R LA D+QSLC  
Sbjct: 138 LVSAIAIVVLGKLPAALALSCLLGVSAAMAIADVVIDACIATNSINIRSLAPDIQSLCMV 197

Query: 192 CSSIGHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGE 251
           CSS G L+GY+TSGVFVH LG Q ALG+LA  PA I++LGFFI+E RSST+ +    K  
Sbjct: 198 CSSAGALVGYATSGVFVHQLGPQGALGVLAFSPATIVILGFFIYEKRSSTVPTQKTKK-- 257

Query: 252 SLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESV 311
               +GVA++  CK +KYP VWKP+L+MF+ L+L+ISTHEG FYWYTD  AGPAFSQE V
Sbjct: 258 DTDGLGVAVKGMCKTVKYPEVWKPSLYMFISLALNISTHEGHFYWYTDPTAGPAFSQEFV 317

Query: 312 GLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPD 371
           G+IYAVG+ AS+ GVLIYHK L+   FR ILFFAQL Y  +G+LD+VFI RWNL L IPD
Sbjct: 318 GIIYAVGALASMFGVLIYHKKLKGYSFRNILFFAQLLYVFSGMLDLVFIKRWNLTLGIPD 377

Query: 372 ELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLV 431
            LFV+ EE  ++++S+IRW PM+VL+TRLC +GIEGTFFA L CIDS G L SKW GG V
Sbjct: 378 SLFVITEESFTKMISKIRWIPMVVLSTRLCPLGIEGTFFAFLMCIDSFGQLASKWGGGFV 437

Query: 432 LHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVE 491
           LHAFGVTR DF NLWLV+L+R++LR V + FVFLVPD+   D LVPSD    ++S    +
Sbjct: 438 LHAFGVTRHDFGNLWLVILIRNILRLVTVCFVFLVPDSDHLDDLVPSDVLPKKQSE---D 497

Query: 492 EDGDSIPLV 495
           +D D I L+
Sbjct: 498 DDDDDIKLL 501

BLAST of CmoCh05G003500 vs. TAIR 10
Match: AT1G79710.1 (Major facilitator superfamily protein )

HSP 1 Score: 425.2 bits (1092), Expect = 7.1e-119
Identity = 216/486 (44.44%), Postives = 316/486 (65.02%), Query Frame = 0

Query: 10  AKRPNSDPSDSNKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSD 69
           ++R + D      L  ++ +P RW +ML  EL+ SF  GV++VYG+S G      KV + 
Sbjct: 14  SRRNHQDKKKEGVLDLVLKRPLRWLRMLIEELHWSFFFGVIIVYGVSQGLGKGLSKVSTQ 73

Query: 70  YYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASA 129
           YY+KD QK+QPS  QIY+G+  IPW++KP+WGLLTD  PV GY RRPYF++AG +   S 
Sbjct: 74  YYFKDEQKIQPSQAQIYVGLIQIPWIIKPLWGLLTDVVPVLGYRRRPYFILAGFLAMISM 133

Query: 130 VAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSI 189
           + + +   L +  AL  L+  SA +AIADVTIDAC+ + SI    LA D+QSLCG CSSI
Sbjct: 134 MVLWLHTNLHLALALSCLVAGSAGVAIADVTIDACVTQCSISHPTLAADMQSLCGLCSSI 193

Query: 190 GHLIGYSTSGVFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQ 249
           G L+G+S SGV VHL+G++   G+L +   +++V+G  + E  S +L    K   +    
Sbjct: 194 GSLVGFSLSGVLVHLVGSKGVYGLLGVTAGLLVVVGMVLKESPSRSLGR--KHVNDKFLD 253

Query: 250 VGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIY 309
            G AI    K  +Y  VW+P LFM L  ++S+  HEG FYWYTD K GP+FS+E+VG I 
Sbjct: 254 AGSAI---WKTFQYGEVWRPCLFMLLSAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGSIM 313

Query: 310 AVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFV 369
           + G+  SL+G+L+Y   L++  FR ++F+A     ++G LD++ +LR NL L +PD  F+
Sbjct: 314 SFGAIGSLVGILLYQNFLKNFPFRNVVFWALSLSVLSGFLDLILVLRINLKLGLPDYFFI 373

Query: 370 VLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAF 429
           V++E VS ++SRI+W P++VL+++LC  G+EGTFFALL  I+ +G L S W GG++LHA 
Sbjct: 374 VVDEFVSHMISRIKWLPLLVLSSKLCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHAL 433

Query: 430 GVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSDQTVSRKSSTTVEEDGD 489
            VTR+ F NLWLV+++RSLLR + +  VFL+P+   +  ++P++   SR S     E  +
Sbjct: 434 KVTRTQFDNLWLVIVIRSLLRVIPIGLVFLIPNVDSNSTILPAEMLTSRISEGVDTEKIE 493

Query: 490 SIPLVS 496
              L+S
Sbjct: 494 MTALIS 494

BLAST of CmoCh05G003500 vs. TAIR 10
Match: AT5G25050.1 (Major facilitator superfamily protein )

HSP 1 Score: 405.2 bits (1040), Expect = 7.6e-113
Identity = 204/453 (45.03%), Postives = 300/453 (66.23%), Query Frame = 0

Query: 25  SIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQ 84
           +++  P RW KMLSSEL+ SF+ GVV +YG++ G  GS  +V ++YY KDVQK+QPS  Q
Sbjct: 25  NVVCGPVRWLKMLSSELHWSFVFGVVSLYGINQGLGGSLGRVATEYYMKDVQKVQPSESQ 84

Query: 85  IYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGAL 144
               I  IPW++KP+WG+LTD  P+ G+ RRPYF++AGV+G  S + +++   L +  AL
Sbjct: 85  ALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFILAGVLGVVSLLFISLHSNLHLYLAL 144

Query: 145 GLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHL 204
             +   SAAMAIADVTIDAC   +SI+   LA D+QSLC   SSIG L+G+  SG+ VHL
Sbjct: 145 FWMTISSAAMAIADVTIDACTAYNSIKHPSLASDMQSLCSLSSSIGALLGFFMSGILVHL 204

Query: 205 LGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQVGVAIRDTCKA---- 264
           +G++   G+L  P A++ V+G    EP            G S KQV     D  KA    
Sbjct: 205 VGSKGVFGLLTFPFALVSVVGIVFSEPH---------VPGFSYKQVNQKFTDAGKAMWRT 264

Query: 265 IKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGV 324
           +K   VW+P+L+M++ L+L ++ HEG FYW+TD K GP F+QE+VG I ++GS  S++  
Sbjct: 265 MKCSDVWRPSLYMYISLTLGLNIHEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILAA 324

Query: 325 LIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFVVLEECVSRIVS 384
            +Y   L+D  FR +  + QL + ++G+LD++ + R NL   +PD LF+V++E VS+++ 
Sbjct: 325 TLYQLVLKDHPFRGLCLWTQLLFALSGMLDLILVFRLNLKFGLPDYLFIVVDEIVSQMIG 384

Query: 385 RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAFGVTRSDFRNLW 444
           R++W P++VL ++LC  GIEGTFFALL  ID+ G++ S W GG++LH   VTR++F NLW
Sbjct: 385 RLKWMPLLVLTSKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGIMLHVLKVTRTEFGNLW 444

Query: 445 LVVLLRSLLRFVVLAFVFLVPDASQSDILVPSD 474
           L VL+R+++R + L F+FLVP   Q+   +P +
Sbjct: 445 LAVLVRNVMRLLPLCFLFLVPKGDQNTFKLPDE 468

BLAST of CmoCh05G003500 vs. TAIR 10
Match: AT5G25040.2 (Major facilitator superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 4.8e-99
Identity = 197/480 (41.04%), Postives = 295/480 (61.46%), Query Frame = 0

Query: 21  NKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQP 80
           + L S++  P RW KML+S+L+ S++  VV   G++ GF  S   V +DYY KDVQK+QP
Sbjct: 21  SNLRSVVCGPVRWLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQP 80

Query: 81  SVVQIYIGIYTIPWVM-KPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLG 140
           S  Q    I  I W++ KP++G+LTD  P+ G+ RRPYF++AGV+G  S + ++++  L 
Sbjct: 81  SQYQALSAITRISWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLH 140

Query: 141 ILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSG 200
           +  AL  +   SAAMAIADVTIDAC   +S +   LA D+QSLC    SIG L+G+  SG
Sbjct: 141 LYLALFWMTISSAAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSG 200

Query: 201 VFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQ-VGVAIRDTC 260
           + VHL+G++   G+L    A++ V+G    EPR           G S KQ    A++   
Sbjct: 201 ILVHLVGSKGVFGLLTFTFALVSVVGVLFSEPR---------VHGFSFKQNFTNAMKAMW 260

Query: 261 KAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFS----------------- 320
           + IK   VW+P+L+MF+  +L +   EG FYW+TD K GP F+                 
Sbjct: 261 RTIKCSDVWQPSLYMFITRALGLDIKEGLFYWFTDSKDGPLFAQVIPKQVASIFSKSTNM 320

Query: 321 --------QESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVF 380
                   QE+VG I ++GS  S++GVL+Y+  L+D  FR++  + QL + ++G+ D++ 
Sbjct: 321 LKAFLVCFQETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLFLWTQLLFALSGMFDLIL 380

Query: 381 ILRWNLVLKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSL 440
           +LR NL   +PD LF+V++  VS+++ R+ W  + VLNT+LC  GIEGTFFALL  ID+ 
Sbjct: 381 VLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCPHGIEGTFFALLMSIDNA 440

Query: 441 GMLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPSD 474
           G++ S W GG +LH   VTR++F NLWL VL+R+++R + + F+FLVP   Q+   +P++
Sbjct: 441 GLMTSSWLGGKMLHVLKVTRTEFGNLWLAVLVRNVMRLLPICFLFLVPQGDQNTFKLPAE 491

BLAST of CmoCh05G003500 vs. TAIR 10
Match: AT5G25040.3 (Major facilitator superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 7.4e-92
Identity = 179/416 (43.03%), Postives = 265/416 (63.70%), Query Frame = 0

Query: 21  NKLLSIMAQPFRWFKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQP 80
           + L S++  P RW KML+S+L+ S++  VV   G++ GF  S   V +DYY KDVQK+QP
Sbjct: 21  SNLRSVVCGPVRWLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQP 80

Query: 81  SVVQIYIGIYTIPWVM-KPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLG 140
           S  Q    I  I W++ KP++G+LTD  P+ G+ RRPYF++AGV+G  S + ++++  L 
Sbjct: 81  SQYQALSAITRISWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLH 140

Query: 141 ILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSG 200
           +  AL  +   SAAMAIADVTIDAC   +S +   LA D+QSLC    SIG L+G+  SG
Sbjct: 141 LYLALFWMTISSAAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSG 200

Query: 201 VFVHLLGAQEALGILAIPPAMIIVLGFFIHEPRSSTLQSYGKPKGESLKQ-VGVAIRDTC 260
           + VHL+G++   G+L    A++ V+G    EPR           G S KQ    A++   
Sbjct: 201 ILVHLVGSKGVFGLLTFTFALVSVVGVLFSEPR---------VHGFSFKQNFTNAMKAMW 260

Query: 261 KAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLI 320
           + IK   VW+P+L+MF+  +L +   EG FYW+TD K GP F+QE+VG I ++GS  S++
Sbjct: 261 RTIKCSDVWQPSLYMFITRALGLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSIL 320

Query: 321 GVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLVLKIPDELFVVLEECVSRI 380
           GVL+Y+  L+D  FR++  + QL + ++G+ D++ +LR NL   +PD LF+V++  VS++
Sbjct: 321 GVLLYNLRLKDHPFRKLFLWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKM 380

Query: 381 VSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHAFGVTRS 435
           + R+ W  + VLNT+LC  GIEGTFFALL  ID+ G++ S W GG +LH     R+
Sbjct: 381 IIRLTWMVIFVLNTKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKEIRT 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LEV71.2e-16860.74Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana OX=3702 GN=At5g1... [more]
Q9SQN21.0e-11744.44Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana OX=3702 GN=At1g7... [more]
Q5FV411.1e-11145.03Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g2... [more]
F4KIL84.5e-10243.30Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana OX=3702 GN=At5g2... [more]
Q9SKZ51.4e-4230.70Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1HEZ63.9e-282100.00probable folate-biopterin transporter 6 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K7V42.8e-27298.18probable folate-biopterin transporter 6 OS=Cucurbita maxima OX=3661 GN=LOC111492... [more]
A0A0A0LMJ98.2e-24086.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G382710 PE=3 SV=1[more]
A0A1S3BD558.2e-24084.77probable folate-biopterin transporter 6 OS=Cucumis melo OX=3656 GN=LOC103488353 ... [more]
A0A5A7V9P73.2e-23686.76Putative folate-biopterin transporter 6 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
XP_022961764.18.0e-282100.00probable folate-biopterin transporter 6 [Cucurbita moschata][more]
XP_023545761.11.3e-27999.20probable folate-biopterin transporter 6 [Cucurbita pepo subsp. pepo][more]
KAG6598542.12.8e-27499.19putative folate-biopterin transporter 6, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022997581.15.7e-27298.18probable folate-biopterin transporter 6 [Cucurbita maxima][more]
XP_038886156.11.6e-25087.11probable folate-biopterin transporter 6 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G10820.18.8e-17060.74Major facilitator superfamily protein [more]
AT1G79710.17.1e-11944.44Major facilitator superfamily protein [more]
AT5G25050.17.6e-11345.03Major facilitator superfamily protein [more]
AT5G25040.24.8e-9941.04Major facilitator superfamily protein [more]
AT5G25040.37.4e-9243.03Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 44..461
e-value: 1.1E-20
score: 75.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 49..458
IPR039309Biopterin transporter familyPFAMPF03092BT1coord: 65..224
e-value: 3.6E-31
score: 108.0
coord: 266..474
e-value: 3.7E-44
score: 150.9
IPR039309Biopterin transporter familyPANTHERPTHR31585FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTICcoord: 11..496
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 476..502
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR31585:SF44FOLATE-BIOPTERIN TRANSPORTER 6-RELATEDcoord: 11..496
NoneNo IPR availableCDDcd17484MFS_FBTcoord: 48..438
e-value: 2.10469E-123
score: 364.626

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G003500.1CmoCh05G003500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane