CmoCh05G002620 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G002620
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPfkB domain-containing protein
LocationCmo_Chr05: 1123859 .. 1127161 (+)
RNA-Seq ExpressionCmoCh05G002620
SyntenyCmoCh05G002620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTCGGAGTCCTATATAAAGGAGGGCAGAGAAAGCAGGACCTTAAGCCACCAAGATGGGAGCCGAGCCTTTGCTTCACACTGCCAATGACTCTGAAGCTGCCCCTCTTATTCTTGGTCTTCAACCTGCTGCCCTTATCGACCATGTTGCTCGTGTCGATTCCTCTCTTCTTGATCGAATTCCCGGCGACCGCGGTGGCTCAATGCCGGTAAACATCCTTCTTTCTTTCGCCTTTTTACTCTACTCGCTCAATTGTGTTTGTTGTGTTCTGTTTTGGATTTCGTTGTGTTTTTAAGATCAGAATTGTATTGACTAATTTCTGTGTTGTTGATTCATCTTTGATTAAGCGGGTGGTTCTGCTTCTGACGACGTTTTTGTTTCTTCTAATGTTTGAATCTCTCTTTGATTATGTGTTCTGTTCTGTGTGGTTTTGGAACTGTCTTTTTACTGTCCAATACAGTCGGATTTCTTACGGTGCTGTGGATTTTGTTTTTTTTCTACTTTTAGGAGCTGAATTCTTGTTGTTTTTGTGCAATGCTCTGTTTTTGTTTTCTTTAATCGAGCATGTGGGGAGGAGAATTTAGTTTATGTTTCTTTTTTCACCATTTTATTCGTATCCAAGTGCTGAATTTCGTCTGTTTTGAGTTTATTTTCAGGTTGGTATGGAAGAGCTTGAGAATATTCTGAGTGAGGTGAAATCCTATATACGTTCCTCGCCTGATGATCTGACGCCGGTCAAGACAATAGCTGGGGGAAGTGTTGCTAACACAATTCGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATCATTGGCGCCTATGGGGATGATGAGCAAGGAAAATTGTTCGTCAGTAACATGGGCTCCAATGGGGTCAATCTCTCAAGATTGAGGATGATCAAAGGTCCCACAGCTCAGGTCTGCTTCACTCCCTTCATAGTGGGGTGTCCAAATTTGCATTCTGTGTTTATGTGTTTTAAAGTTCTGAGAATGTATTGTAAATTGTCCGGCAGTGTGTCTGCCTGGTTGATTCATTGGGCAACCGCACAATGCGACCCTGTCTCTCAAGTGCAGTCAAAGTTCAGGTATATTACCTTCTCAGTTGGGAGTTCGTAACAGCCAAGCACACCGGTTAGCAAATATTGTCTCTTTGGGCTTTCCTTTCAAGGGCCCTCTATTAAGAAGAGGTTTCCACACCCTTACAAGGAATGCTTCATTCCCCTCTCCAACCAATGTGGGATCTCACAATCAACCCTCCTTGGAGGCCTACCATCTTCGCTAGCACACTGCCCGGTATCTAGCTCTGATACCATTTTTAACAAAGGACAATATTTGCTAGCGGTGTGTTTGGGCTGTTATAGAGTTTGTTATAGAGTTTGTGTTGGTTTGTTGAATAAATTGATCAGTTTAGTACTTTTGTATGTTAAACAGATGAGCTTTGGTTACTAAATATGCTGATTATCTATTTGTTTTGTGATCTTTCAGGGCAATGAACTGAGTAGAGATGACTTTAAGGGCTCTAAGGTCAGGATACTCCCATCTTTATTTTCTTTTAACATAAGAGGTAGAGGCTTTTGGTAGACTTAATTATTTCCATGTCTTTATTGCAGTGGCTTGTAATGAGATATAGTATATTTAGCTTAGAAGTTATTCAAGCAGCTGTAAAGATGGCCAAACAAGAGAGAGTTTTAGTATCCTTGGATTTGGCCAGTTTTGAGGTATAGAAATAGACAACTTTGTTATGCCGTTTTAAAAGAAATGTTGAACAAAGTAGCTCACATCTCTATATTTGGCCAGATGGTTCGGAATTTTCGATTGCCTCTTCTCAAGCTATTGGAGTCCGGGGATATCGACCTTTGCTTTGCCAATGAGGACGAAGCAAAGGAGTTGATAGGGTGATGGTCGTTCCTTTTAAGCTCGTTATTCTTCGAACTCGACTGATTGTTAATTTGATATCTGCACTACTTCAGCTGTTGGATATTTCGGTTCTGTGGCAGAACATTTTCTAGTTGATGGTATTAACATCTGTCATCTTTACAGGGGTGAGAAAGACGCTGATCCTGAAGTTGCACTAGAATTTCTTGCAAAGCACTGTCAATGGGCAGTGGTGACATTAGGTGCCAATGGTTGTATCGCTAAGCATGGAAAAGAGGTTAGTGATTGATTTTTCCTCTTATTGGCAACATGTGACGATTCAAACATTAGAATATCTTCTAGTTTCTTTCTCATGAACACAATGTTTCAAGCTTACGCATGGATCGTTGGTAAAATGAGTCTCTTTTTTGTGGATAACTAGCACTCGTGCTTTGCTTTGGAGTCTCTAGGGAGAGGAGTAATAAAGTGTTTAAAGGTGTGGGGAGTGGTCTTGGTAGAGAGTTTCCAAGCCCACTGCTAGCATATATTGTTCGTTTTGGCCCGTTATGTATCACCGTCAACCTCACGCGTTTATTAGGGAGAGATTTTAATTCTCCTCTCCAACCGAGGTGGGATTTCACAATCCACCCCTTGGGGGCGCAACGTCCTCGTTGGCACACCACTCGGTATCTGGCTTTGATACTATTTGTAACAACTCAAGTCCACCACTAGCAGATATATTATCTGGATGAGTATCGGTTCGCTCTATAACTTTGAAAGACGCGTTTTAAAATCTTGAGGGGAAGCCCATAGGGGAAAGTCCTGAGAAGACAATATCTGCTAGCAGTGAGCTTGAGCGGTTAATGTTTCATCCCATGTTAAGGTTCCATATTCTCCCTTTGGCCTTTTTGGTGTGCAAGGACTTTTGTCGGTTAAGGTTGATCTTACTTGATTGGACCCCTTGTCTTTAGCTTGGCTTCTTTTGGGGGTTTGTCTTTTTTATGTCCTTTGTATTCTTTCATTCATTTCAATAGGACATGGAAGTTTCTCGTTGCATCTAGGAAATGTTTAGTTTGTCCAAAGCAATACACAAATTCAAGAGACCATAGAGTGGAACCAAGTGTTCTGAATTCTGATACAATATTTCCATGTGTGGGTCATTAGTAATGAAGTTACATAACTCCCCTCAACATATCAGGTTGTCCGAATTCCAGCGATAGGGGAGTCAAAGGCGACCGATGCGACCGGAGCAGGCGACTTGTTTGCGAGCGGGTTTCTATACGGATTAGTGAAGGGATTATCTCTTGTGAAATGCTGTCAATTGGGATCATGCAGCGGCGGTTCTGTCATCCGTGCCCTTGGGGGTGAGGTTACCCCAGAAAACAGGCAATGGATGCACAAGCATTTGCAGATCAAAGGGCTTCCGCTCCCTGAGATCCCTCAATGA

mRNA sequence

CCCTCGGAGTCCTATATAAAGGAGGGCAGAGAAAGCAGGACCTTAAGCCACCAAGATGGGAGCCGAGCCTTTGCTTCACACTGCCAATGACTCTGAAGCTGCCCCTCTTATTCTTGGTCTTCAACCTGCTGCCCTTATCGACCATGTTGCTCGTGTCGATTCCTCTCTTCTTGATCGAATTCCCGGCGACCGCGGTGGCTCAATGCCGGTTGGTATGGAAGAGCTTGAGAATATTCTGAGTGAGGTGAAATCCTATATACGTTCCTCGCCTGATGATCTGACGCCGGTCAAGACAATAGCTGGGGGAAGTGTTGCTAACACAATTCGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATCATTGGCGCCTATGGGGATGATGAGCAAGGAAAATTGTTCGTCAGTAACATGGGCTCCAATGGGGTCAATCTCTCAAGATTGAGGATGATCAAAGGTCCCACAGCTCAGTGTGTCTGCCTGGTTGATTCATTGGGCAACCGCACAATGCGACCCTGTCTCTCAAGTGCAGTCAAAGTTCAGGGCAATGAACTGAGTAGAGATGACTTTAAGGGCTCTAAGTGGCTTGTAATGAGATATAGTATATTTAGCTTAGAAGTTATTCAAGCAGCTGTAAAGATGGCCAAACAAGAGAGAGTTTTAGTATCCTTGGATTTGGCCAGTTTTGAGATGGTTCGGAATTTTCGATTGCCTCTTCTCAAGCTATTGGAGTCCGGGGATATCGACCTTTGCTTTGCCAATGAGGACGAAGCAAAGGAGTTGATAGGGGGTGAGAAAGACGCTGATCCTGAAGTTGCACTAGAATTTCTTGCAAAGCACTGTCAATGGGCAGTGGTGACATTAGGTGCCAATGGTTGTATCGCTAAGCATGGAAAAGAGGTTGTCCGAATTCCAGCGATAGGGGAGTCAAAGGCGACCGATGCGACCGGAGCAGGCGACTTGTTTGCGAGCGGGTTTCTATACGGATTAGTGAAGGGATTATCTCTTGTGAAATGCTGTCAATTGGGATCATGCAGCGGCGGTTCTGTCATCCGTGCCCTTGGGGGTGAGGTTACCCCAGAAAACAGGCAATGGATGCACAAGCATTTGCAGATCAAAGGGCTTCCGCTCCCTGAGATCCCTCAATGA

Coding sequence (CDS)

ATGGGAGCCGAGCCTTTGCTTCACACTGCCAATGACTCTGAAGCTGCCCCTCTTATTCTTGGTCTTCAACCTGCTGCCCTTATCGACCATGTTGCTCGTGTCGATTCCTCTCTTCTTGATCGAATTCCCGGCGACCGCGGTGGCTCAATGCCGGTTGGTATGGAAGAGCTTGAGAATATTCTGAGTGAGGTGAAATCCTATATACGTTCCTCGCCTGATGATCTGACGCCGGTCAAGACAATAGCTGGGGGAAGTGTTGCTAACACAATTCGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATCATTGGCGCCTATGGGGATGATGAGCAAGGAAAATTGTTCGTCAGTAACATGGGCTCCAATGGGGTCAATCTCTCAAGATTGAGGATGATCAAAGGTCCCACAGCTCAGTGTGTCTGCCTGGTTGATTCATTGGGCAACCGCACAATGCGACCCTGTCTCTCAAGTGCAGTCAAAGTTCAGGGCAATGAACTGAGTAGAGATGACTTTAAGGGCTCTAAGTGGCTTGTAATGAGATATAGTATATTTAGCTTAGAAGTTATTCAAGCAGCTGTAAAGATGGCCAAACAAGAGAGAGTTTTAGTATCCTTGGATTTGGCCAGTTTTGAGATGGTTCGGAATTTTCGATTGCCTCTTCTCAAGCTATTGGAGTCCGGGGATATCGACCTTTGCTTTGCCAATGAGGACGAAGCAAAGGAGTTGATAGGGGGTGAGAAAGACGCTGATCCTGAAGTTGCACTAGAATTTCTTGCAAAGCACTGTCAATGGGCAGTGGTGACATTAGGTGCCAATGGTTGTATCGCTAAGCATGGAAAAGAGGTTGTCCGAATTCCAGCGATAGGGGAGTCAAAGGCGACCGATGCGACCGGAGCAGGCGACTTGTTTGCGAGCGGGTTTCTATACGGATTAGTGAAGGGATTATCTCTTGTGAAATGCTGTCAATTGGGATCATGCAGCGGCGGTTCTGTCATCCGTGCCCTTGGGGGTGAGGTTACCCCAGAAAACAGGCAATGGATGCACAAGCATTTGCAGATCAAAGGGCTTCCGCTCCCTGAGATCCCTCAATGA

Protein sequence

MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENILSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMGSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVMRYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGAGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLPEIPQ
Homology
BLAST of CmoCh05G002620 vs. ExPASy Swiss-Prot
Match: Q5JDG9 (ADP-dependent ribose-1-phosphate kinase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=TK2029 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.4e-17
Identity = 83/281 (29.54%), Postives = 127/281 (45.20%), Query Frame = 0

Query: 83  GGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMGSNGVNLSRLRMIKGPTAQCVC 142
           GG+ ANTI  L A FG+  G IGA G+D+ G++ +      GV+   + +++ P+   V 
Sbjct: 39  GGAAANTISWL-AHFGLKTGYIGAVGNDDVGEMHIKYFQGIGVDTGGIDVVEEPSGVAVA 98

Query: 143 LVDSLGNRTMRPCLSSAVKVQGNELSRDDFK---GSKWLVMRYSIFSLEVIQAAVKMAKQ 202
           +V     R         VK  G  L R  FK    S+   +  S    E+I+ AV  A Q
Sbjct: 99  MVAGDDKR--------IVKYPGANLRR-RFKPEYASRAKFLHLSSNPPELIEEAVNFASQ 158

Query: 203 ERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEAKELIGGEKDADPEVALEFL 262
             + VSLD+           PL + LES  +D    NEDE +   G     DP +     
Sbjct: 159 RGIKVSLDIG--------EAPLPRELES-KVDYLMMNEDEYRRKYG---SLDPSLC---- 218

Query: 263 AKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGAGDLFASGFLYGLVKGLSLV 322
               +  VVTL   G + + G  V  +  +  +K  D+TGAGD F +G +YG++ G SL+
Sbjct: 219 --RAKNLVVTLNGGGALVREGDNVFEVRGL-SAKVVDSTGAGDSFDAGVIYGVLNGWSLL 278

Query: 323 KCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 361
              +LG       ++ +G        + + +  +  GL LP
Sbjct: 279 DSAKLGMLLAYLTVQKVGARSAIVPLEEVKRIAREVGLDLP 290

BLAST of CmoCh05G002620 vs. ExPASy Swiss-Prot
Match: Q55480 (Uncharacterized sugar kinase slr0537 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0537 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.2e-16
Identity = 81/281 (28.83%), Postives = 131/281 (46.62%), Query Frame = 0

Query: 79  KTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMGSNGV--NLSRLRMIKGP 138
           K  +GGS ANT+  L A  G +       G DE G  ++ ++   G+  N       +G 
Sbjct: 57  KQSSGGSAANTLVSL-AQLGGTGFYACKVGKDEAGAFYLQDLNDCGLDTNPHHETAGEGI 116

Query: 139 TAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVMR-YSIFSLEVIQAAVK 198
           T +C+  V    +RTM   L  +  +   E+     K S++L +  Y + S     A ++
Sbjct: 117 TGKCLVFVTPDADRTMNAFLGISGSLSVTEMDWSALKQSQYLYLEGYLVTSPSAKAACIE 176

Query: 199 ---MAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEAKELIGGEKDADP 258
              +A+Q  V   L L+   M + F+  L ++L SG +DL FANE EA E+ G    +D 
Sbjct: 177 AKAIAEQSGVKTCLSLSDPNMAKFFQDGLKEMLGSG-VDLLFANEAEALEMAG---TSDL 236

Query: 259 EVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGAGDLFASGFLYGL 318
             A+ +     +   +T G  G +   G+ ++ I    + +  D  GAGD++A GFLYGL
Sbjct: 237 NQAIAYCKSIAKNFALTRGGAGSLIFDGENLLTI-GTPKVQPIDTVGAGDMYAGGFLYGL 296

Query: 319 VKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQ 354
             G+   K  QL S +   V+   G  +  E  Q + + +Q
Sbjct: 297 THGMDYEKAGQLASETAAKVVTCYGPRLDTEILQEILQSVQ 331

BLAST of CmoCh05G002620 vs. ExPASy Swiss-Prot
Match: D9TT10 (ATP-dependent 6-phosphofructokinase OS=Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) OX=580327 GN=pfkB PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.6e-11
Identity = 68/270 (25.19%), Postives = 122/270 (45.19%), Query Frame = 0

Query: 79  KTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMGSNGVNLSRLRMIKGPTA 138
           K   GGS AN I   S   G++  I+ + G+D  GK  +  +  + + +  +R +   T+
Sbjct: 42  KKYFGGSPAN-IAINSKMLGINSIIVSSVGNDGLGKFLLKKLQEHHIEIKYVRQVDYSTS 101

Query: 139 QCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSK------WLVMRYSIFSLEVIQA 198
             V +  S  + T      +   ++ ++  +   + +K      W + R    S   ++ 
Sbjct: 102 M-VLVTKSKSSPTPIFYRDADYHIEYSDELKYLIENTKIVHFSSWPISRNP--SRSTVEI 161

Query: 199 AVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLES--GDIDLCFANEDEAKELIGGEKDA 258
            +   K+  VLV  D     M+        + ++S    +D+   +ED+A+ + G  KD 
Sbjct: 162 LIDECKKYDVLVCYDPNYHSMIWERGHDGREYIKSLIAKVDIIKPSEDDAERIFG--KDT 221

Query: 259 DPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGAGDLFASGFLY 318
                 +FL    +  ++TLG +G I  +G+E +R   + + +  D TGAGD F SGF  
Sbjct: 222 PENQLKKFLDLGAKLVILTLGKDGAIVSNGEETIRFNTLAD-EVVDTTGAGDAFWSGFYS 281

Query: 319 GLVKGLSLVKCCQLGSCSGGSVIRALGGEV 341
           GL+KG +L K  +LG       +R +G  V
Sbjct: 282 GLIKGYTLKKSLELGFAVSAYKLRYVGAIV 304

BLAST of CmoCh05G002620 vs. ExPASy Swiss-Prot
Match: C3PAZ0 (5-dehydro-2-deoxygluconokinase OS=Bacillus anthracis (strain A0248) OX=592021 GN=iolC PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.7e-10
Identity = 71/275 (25.82%), Postives = 119/275 (43.27%), Query Frame = 0

Query: 83  GGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMGSNGVNLSRLRM---------- 142
           GGS AN   G +A  G+  G IG   DD+ G+     +  N +N  ++ +          
Sbjct: 44  GGSPANIAIG-AARLGLQTGFIGKVSDDQMGRFITGYLKDNKINTDQIPIDCTGAVTGLA 103

Query: 143 ---IKGPTAQCVCLV--DSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVMRYSIF-- 202
              IK P   C  L+  D++ +  + P           E+S D  K SK L++  +    
Sbjct: 104 FTEIKSP-EDCSILMYRDNVADLNLDP----------TEVSEDYIKQSKALLISGTALAK 163

Query: 203 --SLEVIQAAVKMAKQERVLVSLDL----ASFEMVRNFRLPLLKLLESGDIDLCFANE-D 262
             S E +  A++ A++  V+V  D+     +++      +      E  D+ +    E D
Sbjct: 164 SPSREAVFLALEYARKHDVVVFFDVDYRPYTWQSEAETAVYYNLAAEKSDVIIGTREEFD 223

Query: 263 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDAT 322
             ++L+  EK  D   A  + + H +  V+  G +G IA           I ++K     
Sbjct: 224 MMEKLLNYEKSNDQVTAERWFSHHAKIVVIKHGGDGSIAYTRDGQSHRGGIFKTKVLKTF 283

Query: 323 GAGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVI 334
           GAGD +AS F+YGL++GL + +  +LG  S   VI
Sbjct: 284 GAGDSYASAFIYGLMQGLEIPQAMRLGGASASIVI 306

BLAST of CmoCh05G002620 vs. ExPASy Swiss-Prot
Match: C3LHY4 (5-dehydro-2-deoxygluconokinase OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) OX=568206 GN=iolC PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.7e-10
Identity = 71/275 (25.82%), Postives = 119/275 (43.27%), Query Frame = 0

Query: 83  GGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMGSNGVNLSRLRM---------- 142
           GGS AN   G +A  G+  G IG   DD+ G+     +  N +N  ++ +          
Sbjct: 44  GGSPANIAIG-AARLGLQTGFIGKVSDDQMGRFITGYLKDNKINTDQIPIDCTGAVTGLA 103

Query: 143 ---IKGPTAQCVCLV--DSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVMRYSIF-- 202
              IK P   C  L+  D++ +  + P           E+S D  K SK L++  +    
Sbjct: 104 FTEIKSP-EDCSILMYRDNVADLNLDP----------TEVSEDYIKQSKALLISGTALAK 163

Query: 203 --SLEVIQAAVKMAKQERVLVSLDL----ASFEMVRNFRLPLLKLLESGDIDLCFANE-D 262
             S E +  A++ A++  V+V  D+     +++      +      E  D+ +    E D
Sbjct: 164 SPSREAVFLALEYARKHDVVVFFDVDYRPYTWQSEAETAVYYNLAAEKSDVIIGTREEFD 223

Query: 263 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDAT 322
             ++L+  EK  D   A  + + H +  V+  G +G IA           I ++K     
Sbjct: 224 MMEKLLNYEKSNDQVTAERWFSHHAKIVVIKHGGDGSIAYTRDGQSHRGGIFKTKVLKTF 283

Query: 323 GAGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVI 334
           GAGD +AS F+YGL++GL + +  +LG  S   VI
Sbjct: 284 GAGDSYASAFIYGLMQGLEIPQAMRLGGASASIVI 306

BLAST of CmoCh05G002620 vs. ExPASy TrEMBL
Match: A0A6J1HF87 (uncharacterized protein LOC111462499 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462499 PE=4 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 8.1e-205
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI
Sbjct: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60

Query: 61  LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120
           LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM
Sbjct: 61  LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120

Query: 121 GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM 180
           GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM
Sbjct: 121 GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM 180

Query: 181 RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240
           RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA
Sbjct: 181 RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240

Query: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA 300
           KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA
Sbjct: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA 300

Query: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360
           GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP
Sbjct: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360

Query: 361 EIPQ 365
           EIPQ
Sbjct: 361 EIPQ 364

BLAST of CmoCh05G002620 vs. ExPASy TrEMBL
Match: A0A6J1K5H0 (uncharacterized protein LOC111491853 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491853 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 1.4e-201
Identity = 358/364 (98.35%), Postives = 360/364 (98.90%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI
Sbjct: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60

Query: 61  LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120
           L EVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM
Sbjct: 61  LREVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120

Query: 121 GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM 180
           GSNGVNLSRLRMIKG TAQCVCLVDSLGNRTMRPCLSSAVKV+GNELS DDFKGSKWLVM
Sbjct: 121 GSNGVNLSRLRMIKGSTAQCVCLVDSLGNRTMRPCLSSAVKVKGNELSSDDFKGSKWLVM 180

Query: 181 RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240
           RYSIFSLEVIQAAVKMAKQERV VSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA
Sbjct: 181 RYSIFSLEVIQAAVKMAKQERVFVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240

Query: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA 300
           KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVR+PAIGESKATDATGA
Sbjct: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRVPAIGESKATDATGA 300

Query: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360
           GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP
Sbjct: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360

Query: 361 EIPQ 365
           EIPQ
Sbjct: 361 EIPQ 364

BLAST of CmoCh05G002620 vs. ExPASy TrEMBL
Match: A0A0A0LPB8 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361810 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.6e-189
Identity = 338/365 (92.60%), Postives = 352/365 (96.44%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHT NDSEA+PLILGLQPAALIDHV+RVDSSLLDRIPGDRGGSMPVGMEELENI
Sbjct: 1   MGAEPLLHTVNDSEASPLILGLQPAALIDHVSRVDSSLLDRIPGDRGGSMPVGMEELENI 60

Query: 61  LSEVKSYIRSSPDDL-TPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSN 120
           L EVKSYI SSPDDL TPVKTIAGGSVANTIRGLSAGFG+SCGIIGA GDDEQGKLFVSN
Sbjct: 61  LREVKSYILSSPDDLTTPVKTIAGGSVANTIRGLSAGFGISCGIIGACGDDEQGKLFVSN 120

Query: 121 MGSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLV 180
           M S+GVNLSRLRMIKGPTAQCVCLVD+LGNRTMRP LSSAVKVQGNEL+RDDFKGSKWLV
Sbjct: 121 MSSHGVNLSRLRMIKGPTAQCVCLVDALGNRTMRPSLSSAVKVQGNELTRDDFKGSKWLV 180

Query: 181 MRYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDE 240
           MRYSIF++EVI+AAVKMAKQE+V VSLDLASFEMVR+FRLPLL+LLESGDIDLCFANEDE
Sbjct: 181 MRYSIFNIEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRLPLLQLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATG 300
           AKELI GEKDADPEVALEFLAKHCQWA VTLGANGCIAKHGKE+VR+PAIGESKATDATG
Sbjct: 241 AKELIRGEKDADPEVALEFLAKHCQWAAVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPL 360
           AGDLFASGFLYGLVKGLSL KCCQLGSCSGGSVIRALGGEVTPEN QWMHKHLQIKGLPL
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPL 360

Query: 361 PEIPQ 365
           PEI Q
Sbjct: 361 PEIRQ 365

BLAST of CmoCh05G002620 vs. ExPASy TrEMBL
Match: A0A6J1CTD9 (uncharacterized protein LOC111014039 OS=Momordica charantia OX=3673 GN=LOC111014039 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.8e-188
Identity = 336/366 (91.80%), Postives = 351/366 (95.90%), Query Frame = 0

Query: 1   MGAEPL--LHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELE 60
           MGAEPL  LHT +DSE APL+LGLQPAAL+DH+ARVDSSLLDRIPGDRGGS+PVGMEELE
Sbjct: 1   MGAEPLLQLHTHHDSETAPLVLGLQPAALVDHLARVDSSLLDRIPGDRGGSIPVGMEELE 60

Query: 61  NILSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVS 120
           +IL +VKSYI SSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGA GDDEQGKLFV 
Sbjct: 61  HILRDVKSYILSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGACGDDEQGKLFVC 120

Query: 121 NMGSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWL 180
           NM SNGVNLSRLRM KG TAQCVCLVD+LGNRTMRP LSSAVKVQGNEL+RDDFKGSKWL
Sbjct: 121 NMSSNGVNLSRLRMTKGSTAQCVCLVDALGNRTMRPSLSSAVKVQGNELTRDDFKGSKWL 180

Query: 181 VMRYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANED 240
           VMRYSIF+LEVIQAAVKMAKQE+V VSLDLASFEMVR+FRLPLL+LLESGDIDLCFANED
Sbjct: 181 VMRYSIFNLEVIQAAVKMAKQEKVFVSLDLASFEMVRDFRLPLLQLLESGDIDLCFANED 240

Query: 241 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDAT 300
           EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKE+VR+PAIGESKATDAT
Sbjct: 241 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300

Query: 301 GAGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLP 360
           GAGDLFASGFLYGLVKGLSL KCCQLGSCSGGSVIRALGGEVTPEN QWMHKHLQIKGLP
Sbjct: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLP 360

Query: 361 LPEIPQ 365
           LPEIPQ
Sbjct: 361 LPEIPQ 366

BLAST of CmoCh05G002620 vs. ExPASy TrEMBL
Match: A0A5A7VIC5 (Putative sugar kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005570 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.5e-187
Identity = 336/365 (92.05%), Postives = 351/365 (96.16%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHT NDSEAAPLILGLQPAALIDHV+RVDSSLLDRIPGDRGGSMPVGMEELE+I
Sbjct: 1   MGAEPLLHTVNDSEAAPLILGLQPAALIDHVSRVDSSLLDRIPGDRGGSMPVGMEELESI 60

Query: 61  LSEVKSYIRSSPDDL-TPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSN 120
           L EVKSYI SSPDDL TPVKTIAGGSVANTIRGLSAGFGVSCGIIGA GDDEQGKLFVSN
Sbjct: 61  LREVKSYILSSPDDLTTPVKTIAGGSVANTIRGLSAGFGVSCGIIGACGDDEQGKLFVSN 120

Query: 121 MGSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLV 180
           M S+GVNLSRLRMIKGPTAQCVCLVD+LGNRTMRP LSSAVKVQGNEL+R DFKGSKWLV
Sbjct: 121 MSSHGVNLSRLRMIKGPTAQCVCLVDALGNRTMRPSLSSAVKVQGNELTRHDFKGSKWLV 180

Query: 181 MRYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDE 240
           MRYSIF++EVI+AAVKMAKQE+V VSLDLASFEMVR+F+LPLL LLESG+IDLCFANEDE
Sbjct: 181 MRYSIFNIEVIEAAVKMAKQEKVFVSLDLASFEMVRDFKLPLLHLLESGNIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATG 300
           AKELI GEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGK++VR+PAIGESKATDATG
Sbjct: 241 AKELIRGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKDIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPL 360
           AGDLFASGFLYGLVKGLSL KCCQLGSCSGGSVIRALGGEVTPEN QWMHKHLQIKGLPL
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPL 360

Query: 361 PEIPQ 365
           PEI Q
Sbjct: 361 PEIRQ 365

BLAST of CmoCh05G002620 vs. NCBI nr
Match: XP_022961859.1 (uncharacterized protein LOC111462499 isoform X1 [Cucurbita moschata])

HSP 1 Score: 722.6 bits (1864), Expect = 1.7e-204
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI
Sbjct: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60

Query: 61  LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120
           LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM
Sbjct: 61  LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120

Query: 121 GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM 180
           GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM
Sbjct: 121 GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM 180

Query: 181 RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240
           RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA
Sbjct: 181 RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240

Query: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA 300
           KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA
Sbjct: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA 300

Query: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360
           GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP
Sbjct: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360

Query: 361 EIPQ 365
           EIPQ
Sbjct: 361 EIPQ 364

BLAST of CmoCh05G002620 vs. NCBI nr
Match: KAG6598459.1 (hypothetical protein SDJN03_08237, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 716.8 bits (1849), Expect = 9.2e-203
Identity = 359/364 (98.63%), Postives = 363/364 (99.73%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI
Sbjct: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60

Query: 61  LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120
           L+EVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM
Sbjct: 61  LTEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120

Query: 121 GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM 180
           GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM
Sbjct: 121 GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM 180

Query: 181 RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240
           RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA
Sbjct: 181 RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240

Query: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA 300
           KELIGGEKDADPEVAL FLAKHCQWAVVTLGANGCIAKHGKEVVR+PAIGESKATDATGA
Sbjct: 241 KELIGGEKDADPEVALAFLAKHCQWAVVTLGANGCIAKHGKEVVRVPAIGESKATDATGA 300

Query: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360
           GDLFASGFLYGLVKGLSLV+CCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQ+KGLPLP
Sbjct: 301 GDLFASGFLYGLVKGLSLVECCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQVKGLPLP 360

Query: 361 EIPQ 365
           EIPQ
Sbjct: 361 EIPQ 364

BLAST of CmoCh05G002620 vs. NCBI nr
Match: XP_022996681.1 (uncharacterized protein LOC111491853 isoform X1 [Cucurbita maxima])

HSP 1 Score: 711.8 bits (1836), Expect = 2.9e-201
Identity = 358/364 (98.35%), Postives = 360/364 (98.90%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI
Sbjct: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60

Query: 61  LSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120
           L EVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM
Sbjct: 61  LREVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNM 120

Query: 121 GSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVM 180
           GSNGVNLSRLRMIKG TAQCVCLVDSLGNRTMRPCLSSAVKV+GNELS DDFKGSKWLVM
Sbjct: 121 GSNGVNLSRLRMIKGSTAQCVCLVDSLGNRTMRPCLSSAVKVKGNELSSDDFKGSKWLVM 180

Query: 181 RYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240
           RYSIFSLEVIQAAVKMAKQERV VSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA
Sbjct: 181 RYSIFSLEVIQAAVKMAKQERVFVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEA 240

Query: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGA 300
           KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVR+PAIGESKATDATGA
Sbjct: 241 KELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRVPAIGESKATDATGA 300

Query: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360
           GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP
Sbjct: 301 GDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLP 360

Query: 361 EIPQ 365
           EIPQ
Sbjct: 361 EIPQ 364

BLAST of CmoCh05G002620 vs. NCBI nr
Match: XP_038886551.1 (uncharacterized sugar kinase slr0537 [Benincasa hispida])

HSP 1 Score: 684.9 bits (1766), Expect = 3.9e-193
Identity = 343/365 (93.97%), Postives = 356/365 (97.53%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHTANDSEAAPLILGLQPAALIDHV+RVDSSLL+RIPGDRGGSMPVGMEELENI
Sbjct: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVSRVDSSLLNRIPGDRGGSMPVGMEELENI 60

Query: 61  LSEVKSYIRSSP-DDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSN 120
           L EVKSY  S+P DDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSN
Sbjct: 61  LREVKSYTLSTPDDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSN 120

Query: 121 MGSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLV 180
           M SNGVNLSRLRMIKGPTAQCVCLVD+LGNRTMRPCLSSAVKVQGNEL+RDDFKGSKWLV
Sbjct: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDALGNRTMRPCLSSAVKVQGNELTRDDFKGSKWLV 180

Query: 181 MRYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDE 240
           MRYSIF++EVI+AAVKMAKQE+V VSLDLASFEMVR+FRLPLL+LLESGDIDLCFANEDE
Sbjct: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRLPLLQLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATG 300
           AKELIGGE DADPEVALEFLAKHCQWAVVTLGANGCIAKHGKE+VR+PAIGESKATDATG
Sbjct: 241 AKELIGGETDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPL 360
           AGDLFASGFLYGLVKGLSL KCCQLGSCSGGSVIRALGGEVTPEN QWMHKHLQIKGLPL
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPL 360

Query: 361 PEIPQ 365
           PEIPQ
Sbjct: 361 PEIPQ 365

BLAST of CmoCh05G002620 vs. NCBI nr
Match: XP_004152687.1 (uncharacterized protein LOC101214597 [Cucumis sativus] >KGN62617.1 hypothetical protein Csa_021844 [Cucumis sativus])

HSP 1 Score: 671.8 bits (1732), Expect = 3.4e-189
Identity = 338/365 (92.60%), Postives = 352/365 (96.44%), Query Frame = 0

Query: 1   MGAEPLLHTANDSEAAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENI 60
           MGAEPLLHT NDSEA+PLILGLQPAALIDHV+RVDSSLLDRIPGDRGGSMPVGMEELENI
Sbjct: 1   MGAEPLLHTVNDSEASPLILGLQPAALIDHVSRVDSSLLDRIPGDRGGSMPVGMEELENI 60

Query: 61  LSEVKSYIRSSPDDL-TPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSN 120
           L EVKSYI SSPDDL TPVKTIAGGSVANTIRGLSAGFG+SCGIIGA GDDEQGKLFVSN
Sbjct: 61  LREVKSYILSSPDDLTTPVKTIAGGSVANTIRGLSAGFGISCGIIGACGDDEQGKLFVSN 120

Query: 121 MGSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLV 180
           M S+GVNLSRLRMIKGPTAQCVCLVD+LGNRTMRP LSSAVKVQGNEL+RDDFKGSKWLV
Sbjct: 121 MSSHGVNLSRLRMIKGPTAQCVCLVDALGNRTMRPSLSSAVKVQGNELTRDDFKGSKWLV 180

Query: 181 MRYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDE 240
           MRYSIF++EVI+AAVKMAKQE+V VSLDLASFEMVR+FRLPLL+LLESGDIDLCFANEDE
Sbjct: 181 MRYSIFNIEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRLPLLQLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATG 300
           AKELI GEKDADPEVALEFLAKHCQWA VTLGANGCIAKHGKE+VR+PAIGESKATDATG
Sbjct: 241 AKELIRGEKDADPEVALEFLAKHCQWAAVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPL 360
           AGDLFASGFLYGLVKGLSL KCCQLGSCSGGSVIRALGGEVTPEN QWMHKHLQIKGLPL
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPL 360

Query: 361 PEIPQ 365
           PEI Q
Sbjct: 361 PEIRQ 365

BLAST of CmoCh05G002620 vs. TAIR 10
Match: AT1G19600.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 509.6 bits (1311), Expect = 2.1e-144
Identity = 252/347 (72.62%), Postives = 300/347 (86.46%), Query Frame = 0

Query: 16  APLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENILSEVKSYIRSSPDDL 75
           APL+LGLQPAALID+VA VD SLLD+IPGDRGGS+ V  +ELE++L E+ ++I      +
Sbjct: 12  APLVLGLQPAALIDNVAPVDWSLLDQIPGDRGGSIAVQKDELEHMLKELDAHI-----SV 71

Query: 76  TPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMGSNGVNLSRLRMIKG 135
            P+K +AGGSV NT+RGLS GFGV+ GIIGAYGDDEQG+LFVSNMG +GV++SRLR  KG
Sbjct: 72  APLKKMAGGSVTNTVRGLSVGFGVATGIIGAYGDDEQGQLFVSNMGFSGVSISRLRKKKG 131

Query: 136 PTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVMRYSIFSLEVIQAAVK 195
            TAQCVCLVD  GNRTMRPCLSSAVK+Q +ELS++DF GSKWLV+RY++ +L+VIQAA++
Sbjct: 132 STAQCVCLVDDSGNRTMRPCLSSAVKIQADELSKEDFTGSKWLVLRYAVLNLQVIQAAIR 191

Query: 196 MAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEAKELIGGEKDADPEVA 255
            AKQE + VSLDLASFEMVRN +  L +LLESG+IDLCFANEDEA EL+ GE++A PE A
Sbjct: 192 FAKQEGLSVSLDLASFEMVRNSKSELRQLLESGNIDLCFANEDEAAELLRGEQEAGPEAA 251

Query: 256 LEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGAGDLFASGFLYGLVKG 315
           LEFL +HC+WAVVTLG+ GCIAKH KEVV I AIGE+ ATDATGAGDLFASGFLYGL+KG
Sbjct: 252 LEFLGRHCRWAVVTLGSKGCIAKHDKEVVHISAIGETVATDATGAGDLFASGFLYGLIKG 311

Query: 316 LSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLPEI 363
           LSL +CC++GSCSGGSVIRALGGEVTPEN QWMHK LQ+KGLP+P+I
Sbjct: 312 LSLEECCKVGSCSGGSVIRALGGEVTPENWQWMHKQLQLKGLPVPDI 353

BLAST of CmoCh05G002620 vs. TAIR 10
Match: AT4G27600.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 73.6 bits (179), Expect = 3.8e-13
Identity = 78/328 (23.78%), Postives = 131/328 (39.94%), Query Frame = 0

Query: 7   LHTANDSEAAPL---ILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELENILSE 66
           +H  N S A+P    +LGL   A++D    VD   L ++  ++G    +  EE   +L  
Sbjct: 108 VHANNSSAASPERWDVLGL-GQAMVDFSGVVDDEFLKKLGLEKGTRKLINHEERGKLLQA 167

Query: 67  VKSYIRSSPDDLTPVKTIAGGSVANTIRGLS-------AGFGVSCGIIGAYGDDEQGKLF 126
           +         D    K  AGGS++NT+  L+           ++  + G+ G D  G  +
Sbjct: 168 M---------DGCSYKAAAGGSLSNTLVALARLGSQSICDRPLNVAMAGSIGGDPLGSFY 227

Query: 127 VSNMGSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSK 186
            + +    VN     +  G T   + L      RTM     ++  V  +         + 
Sbjct: 228 GTKLRRANVNFLSAPIKDGTTGTVIVLTTPDAQRTMLAYQGTSSVVNYDSCLASLIAKTN 287

Query: 187 WLVMRYSIFSL----EVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDL 246
             V+   +F L      I  A + A +   LV++  +    +         ++     D+
Sbjct: 288 VFVVEGYLFELPDTIRTITKACEEAHRNGALVAVTASDVSCIERHYDDFWDIV-GNYADI 347

Query: 247 CFANEDEAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGES 306
            FAN DEA+       +  P  A  +++    +  VT G NG       E + IP     
Sbjct: 348 VFANSDEARAFCHFSAEESPISATRYMSHFVPFVSVTDGINGSYIGVKGEAIYIPP-SPC 407

Query: 307 KATDATGAGDLFASGFLYGLVKGLSLVK 321
              D  GAGD +ASG LYG+++G+S +K
Sbjct: 408 VPVDTCGAGDAYASGILYGILRGVSDLK 423

BLAST of CmoCh05G002620 vs. TAIR 10
Match: AT5G03300.1 (adenosine kinase 2 )

HSP 1 Score: 64.3 bits (155), Expect = 2.3e-10
Identity = 74/290 (25.52%), Postives = 127/290 (43.79%), Query Frame = 0

Query: 78  VKTIAGGSVANTIRGLSAGFGV--SCGIIGAYGDDEQGKLFVSNMGSNGVNLSRLRMIKG 137
           V+ IAGG+  N+I+       +  +   +G+ G D+ G+    +  + GVN+        
Sbjct: 60  VEYIAGGATQNSIKVAQWMLQIPGATSYMGSIGKDKYGEAMKKDATAAGVNVHYYEDESA 119

Query: 138 PTAQC-VCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLVMRYSIF--------- 197
           PT  C VC+V   G R++   LS+A   + + L     K   W ++  + F         
Sbjct: 120 PTGTCGVCVVG--GERSLIANLSAANCYKVDHLK----KPENWALVEKAKFYYIAGFFLT 179

Query: 198 -SLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDEAK--- 257
            S E IQ   + A     + +++L++  +   F+    K L    +D  F NE EA+   
Sbjct: 180 VSPESIQLVSEHAAANNKVFTMNLSAPFICEFFKDVQEKFLPY--MDFVFGNETEARTFS 239

Query: 258 ELIGGEKDADPEVALEF------LAKHCQWAVVTLGANGCIAKHGKEVVRIPAI--GESK 317
            + G E +   ++A++          + +  V+T GA+  +     +V + P I   + K
Sbjct: 240 RVHGWETEDVEQIAIKISQLPKATGTYKRTTVITQGADPVVVAEDGKVKKYPVIPLPKEK 299

Query: 318 ATDATGAGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPE 344
             D  GAGD F  GF+  LVK  S+ +C + G C   +V+    G   PE
Sbjct: 300 LVDTNGAGDAFVGGFMSQLVKEKSIEECVKAG-CYASNVVIQRSGCTYPE 340

BLAST of CmoCh05G002620 vs. TAIR 10
Match: AT5G51830.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 3.0e-10
Identity = 78/263 (29.66%), Postives = 113/263 (42.97%), Query Frame = 0

Query: 79  KTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMGSNGVNLSRLRM-IKGPT 138
           K   GG+ AN   G+S   G S   IG  GDDE G++    +  N V+ S +R      T
Sbjct: 50  KKAPGGAPANVAVGVSR-LGGSSAFIGKVGDDEFGRMLADILRLNNVDNSGMRFDHNART 109

Query: 139 AQCVCLVDSLGNRTM----RPCLSSAVKVQGNELSRDDFKGSKWLVMRYSIFSL------ 198
           A     +   G R       P  S+ + +  +EL ++  + +K  +  Y   SL      
Sbjct: 110 ALAFVTLRGDGEREFLFFRHP--SADMLLLESELDKNLIQKAK--IFHYGSISLIEEPCR 169

Query: 199 EVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGD---------IDLCFANED 258
                A+K+AK    L+S D        N RLPL    E+            D+   +ED
Sbjct: 170 STQLVAMKIAKAAGSLLSYD-------PNLRLPLWPSEEAARKEIMSIWNLADVIKISED 229

Query: 259 EAKELIGGEKDADPEVALEFL-AKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDA 318
           E   L GG+   D +V L+ L   + +  VV+ G NGC     +   R+  + + K  D 
Sbjct: 230 EITFLTGGDDPYDDDVVLQKLFHPNLKLLVVSEGPNGCRYYTQEFKGRVGGV-KVKPVDT 289

Query: 319 TGAGDLFASGFLYGLVKGLSLVK 321
           TGAGD F SG L  L   L+L+K
Sbjct: 290 TGAGDAFVSGLLNSLASDLTLLK 299

BLAST of CmoCh05G002620 vs. TAIR 10
Match: AT5G58730.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 50.8 bits (120), Expect = 2.6e-06
Identity = 28/77 (36.36%), Postives = 39/77 (50.65%), Query Frame = 0

Query: 261 KHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGAGDLFASGFLYGLVKGLSLVK 320
           KH    VVT G  GC   H  + + +P    +K  D TGAGD F  G + GLV+GL++  
Sbjct: 210 KHLCCVVVTNGEKGCRIYHKDDEMTVPPF-LAKQVDPTGAGDSFLGGLIVGLVEGLTVPD 269

Query: 321 CCQLGSCSGGSVIRALG 338
              LG+  G   +  +G
Sbjct: 270 AALLGNLFGSITVEHIG 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5JDG91.4e-1729.54ADP-dependent ribose-1-phosphate kinase OS=Thermococcus kodakarensis (strain ATC... [more]
Q554801.2e-1628.83Uncharacterized sugar kinase slr0537 OS=Synechocystis sp. (strain PCC 6803 / Kaz... [more]
D9TT101.6e-1125.19ATP-dependent 6-phosphofructokinase OS=Thermoanaerobacterium thermosaccharolytic... [more]
C3PAZ01.7e-1025.825-dehydro-2-deoxygluconokinase OS=Bacillus anthracis (strain A0248) OX=592021 GN... [more]
C3LHY41.7e-1025.825-dehydro-2-deoxygluconokinase OS=Bacillus anthracis (strain CDC 684 / NRRL 3495... [more]
Match NameE-valueIdentityDescription
A0A6J1HF878.1e-205100.00uncharacterized protein LOC111462499 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1K5H01.4e-20198.35uncharacterized protein LOC111491853 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0LPB81.6e-18992.60PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361810 PE=4 S... [more]
A0A6J1CTD91.8e-18891.80uncharacterized protein LOC111014039 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A5A7VIC51.5e-18792.05Putative sugar kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82... [more]
Match NameE-valueIdentityDescription
XP_022961859.11.7e-204100.00uncharacterized protein LOC111462499 isoform X1 [Cucurbita moschata][more]
KAG6598459.19.2e-20398.63hypothetical protein SDJN03_08237, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022996681.12.9e-20198.35uncharacterized protein LOC111491853 isoform X1 [Cucurbita maxima][more]
XP_038886551.13.9e-19393.97uncharacterized sugar kinase slr0537 [Benincasa hispida][more]
XP_004152687.13.4e-18992.60uncharacterized protein LOC101214597 [Cucumis sativus] >KGN62617.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
AT1G19600.12.1e-14472.62pfkB-like carbohydrate kinase family protein [more]
AT4G27600.13.8e-1323.78pfkB-like carbohydrate kinase family protein [more]
AT5G03300.12.3e-1025.52adenosine kinase 2 [more]
AT5G51830.13.0e-1029.66pfkB-like carbohydrate kinase family protein [more]
AT5G58730.12.6e-0636.36pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 24..343
e-value: 7.8E-74
score: 250.7
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 26..338
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 78..337
e-value: 2.1E-38
score: 132.3
NoneNo IPR availablePANTHERPTHR43320SUGAR KINASEcoord: 2..355
NoneNo IPR availablePANTHERPTHR43320:SF1CARBOHYDRATE KINASE-LIKEcoord: 2..355
NoneNo IPR availableCDDcd01168adenosine_kinasecoord: 17..337
e-value: 2.4987E-96
score: 287.203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G002620.1CmoCh05G002620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity