CmoCh05G001430 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh05G001430 vs. ExPASy Swiss-Prot
Match: O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.1e-173
Identity = 340/773 (43.98%), Postives = 484/773 (62.61%), Query Frame = 0

Query: 4   HMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPL 63
           H+ +F W     +FLA   L Q   YIVH+D + MP  F  HH W+S+T+ S+       
Sbjct: 5   HLLLFSWALSAHLFLA---LAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSI------- 64

Query: 64  RTTTSSSSSSLPS--KLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTT 123
           + +  SS     S  KL++ Y + ++GF+A L+  +L ALK  PG++S+ +D +V   TT
Sbjct: 65  KASVPSSVDRFHSAPKLVYSYDNVLHGFSAVLSKDELAALKKLPGFISAYKDRTVEPHTT 124

Query: 124 HSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGT 183
           H+S+FL L+P+SGL P S  G DVI+  +D+G+WPES SF DDG+ +IP RWKG C+ GT
Sbjct: 125 HTSDFLKLNPSSGLWPASGLGQDVIVAVLDSGIWPESASFQDDGMPEIPKRWKGICKPGT 184

Query: 184 HFNASLCNKKLIG-------------------------------------GSYVKEASFF 243
            FNAS+CN+KLIG                                     G++ K  S F
Sbjct: 185 QFNASMCNRKLIGANYFNKGILANDPTVNITMNSARDTDGHGTHCASITAGNFAKGVSHF 244

Query: 244 GYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYE 303
           GY  GTARGVAPRAR+A+YK  + EG   SD+IAA+DQA++DGVD+IS+S G   +PLYE
Sbjct: 245 GYAPGTARGVAPRARLAVYKFSFNEGTFTSDLIAAMDQAVADGVDMISISYGYRFIPLYE 304

Query: 304 DPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGV 363
           D ++IA+F AM +G+ V+ SAGN+GP  G++++G+PW+L VA+G  DR F GT+TL NG+
Sbjct: 305 DAISIASFGAMMKGVLVSASAGNRGPGIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGL 364

Query: 364 SVLGSSLFPLNSAIALSPLPIVF---MGQCHN---LKKLKRVGFKIVVCEDSDEYSLDLQ 423
            + G SLFP  + +  S  P+++   +  C +   L +++     IV+C+D+ ++S  ++
Sbjct: 365 KIRGWSLFPARAFVRDS--PVIYNKTLSDCSSEELLSQVENPENTIVICDDNGDFSDQMR 424

Query: 424 VDNVESAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHK 483
           +  +  A++   +FIS             P + +N   G  + +Y+K S  P A + F +
Sbjct: 425 I--ITRARLKAAIFISEDPGVFRSATFPNPGVVVNKKEGKQVINYVKNSVTPTATITFQE 484

Query: 484 TIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKF 543
           T + TKPAP VA  S+RGPS S   + KPDI+APG  ILA++P NV AT + +  + S  
Sbjct: 485 TYLDTKPAPVVAASSARGPSRSYLGISKPDILAPGVLILAAYPPNVFATSIGTNILLSTD 544

Query: 544 NVL-SGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKV 603
            +L SGTSMA PHAAG+AA+LK AHP WSP+AIRSAMMTTAD +DNT+  IKD  N NK 
Sbjct: 545 YILESGTSMAAPHAAGIAAMLKAAHPEWSPSAIRSAMMTTADPLDNTRKPIKDSDN-NKA 604

Query: 604 ATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNH-CEKAL 663
           ATPL MG+GHV+PN+A+DPGL+YD   +DY NLLC+LN+T+ Q +TI RS+++H C    
Sbjct: 605 ATPLDMGAGHVDPNRALDPGLVYDATPQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPS 664

Query: 664 LDLNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNK 723
            DLNYPSFI   +    +   T + ++FKR VTN+G+  ATY+AK+   K   ++V P  
Sbjct: 665 ADLNYPSFIALYSI---EGNFTLLEQKFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQI 724

Query: 724 LKFKRKNQNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVV 730
           L FK KN+  S+ L IR ++  + +S  V G + WVE  G H V+SPIV + +
Sbjct: 725 LVFKNKNEKQSYTLTIR-YIGDEGQSRNV-GSITWVEQNGNHSVRSPIVTSPI 757

BLAST of CmoCh05G001430 vs. ExPASy Swiss-Prot
Match: Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.5e-154
Identity = 316/762 (41.47%), Postives = 441/762 (57.87%), Query Frame = 0

Query: 5   MRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLR 64
           M M   + L+  F       +T  YI+HMDL+A P PF+ H SW+S TL+SV+ +  P  
Sbjct: 1   MGMTVVIILVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRKP-- 60

Query: 65  TTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSS 124
                       K+I+ Y  +++GF+A LT S+L  LK+ PGYVS  +D  V+  TT S 
Sbjct: 61  ------------KIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSP 120

Query: 125 NFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFN 184
            F+ L+  SG  P+SNYG+ ++IG +DTG+WP+S SF+DDG+  +PS+WKG CE     +
Sbjct: 121 KFIGLNSTSGTWPVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---S 180

Query: 185 ASLCNKKLIG-----------------------------------------GSYVKEASF 244
           +SLCNKKLIG                                         G++VK AS+
Sbjct: 181 SSLCNKKLIGAKVFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASY 240

Query: 245 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGL------ 304
           F Y QGTA G+AP A +AIYKA W+EG   SDVIAAIDQAI DGV VISLSLGL      
Sbjct: 241 FSYAQGTASGIAPHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDD 300

Query: 305 --DGVPLYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFG 364
             DG  L  DP+A+A+FAA+++G+FV TS GN GP + ++ +GAPW++ V AGT+ R F 
Sbjct: 301 DNDGFGLENDPIAVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQ 360

Query: 365 GTITLTNGVSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSL 424
           GT+T  N VS    SLFP        P+  +  G   N    K +  +IVVC  ++  ++
Sbjct: 361 GTLTFGNRVSFSFPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINI 420

Query: 425 DLQVDNVESAKIAVGVFISN-ISDWDNLIQTSFPSIFLNAYHGNVIKDYI-KRSSNPKAR 484
             ++  + S   A  V I++ + +  + I+  FP  F+ + H   I+ Y     +N  A+
Sbjct: 421 GSKLHQIRSTGAAAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAK 480

Query: 485 VNFHKTIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRP 544
           + F KT+IGTKPAP V  YSSRGP  S P +LKPDI+APG  IL++WP     T   + P
Sbjct: 481 LEFRKTVIGTKPAPEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALP 540

Query: 545 IYSKFNVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGN 604
           ++S FN+L+GTSMA PH AGVAAL+K  HP WSP+AI+SA+MTTA  +DN          
Sbjct: 541 LFSGFNLLTGTSMAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN---------- 600

Query: 605 KNKVATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLC-ALNYTKNQIQTITRST-SNH 664
                 PLA+G+GHV+ NK ++PGLIYD   +D+ N LC     ++  I  ITRS  S+ 
Sbjct: 601 ------PLAVGAGHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDA 660

Query: 665 CEKALLDLNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVT 714
           C+K    LNYPS I    +  S        + FKR +TN+GE   +Y  ++  +KGL V 
Sbjct: 661 CKKPSPYLNYPSIIAYFTSDQSS------PKIFKRTLTNVGEAKRSYIVRVRGLKGLNVV 715

BLAST of CmoCh05G001430 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.7e-131
Identity = 302/797 (37.89%), Postives = 428/797 (53.70%), Query Frame = 0

Query: 5   MRMFFWVSLMVIFLARSTLTQTDN---YIVHMDLAAMPKPFATHHSWYSATLSSVLLDTS 64
           M  FF+   ++   + S+   + N   YIVH+D  A P  F TH  WY+++L+S+     
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----- 60

Query: 65  PLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTT 124
                     +S P  +IH Y    +GF+A LT      L + P  +S + +      TT
Sbjct: 61  ----------TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTT 120

Query: 125 HSSNFLAL--SPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECES 184
            S  FL L  +  +GLL  S++GSD++IG +DTGVWPE  SF+D G+  +P +WKG+C +
Sbjct: 121 RSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIA 180

Query: 185 GTHFNASLCNKKLIG---------------------------------------GSYVKE 244
              F  S CN+KL+G                                       G YV  
Sbjct: 181 SQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFP 240

Query: 245 ASFFGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGV 304
           AS  GY  G A G+AP+AR+A YK  W  G   SD++AA D A++DGVDVISLS+G   V
Sbjct: 241 ASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVV 300

Query: 305 PLYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITL 364
           P Y D +AI  F A++RGIFV+ SAGN GP   TV + APW+  V AGT+DRDF   + L
Sbjct: 301 PYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKL 360

Query: 365 TNGVSVLGSSLFPLNSAIALSPLPIVFMGQ------------CHNLKKLKRVGFKIVVCE 424
            NG  + G S++           P+V+ G                      V  KIV+C+
Sbjct: 361 GNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCD 420

Query: 425 DSDEYSLDLQVDNVESAKIAVGVFISN-ISDWDNLIQTS--FPSIFLNAYHGNVIKDYIK 484
                +       +      +G+ I+N + D + L+      P+  + A  G+ I+ YI 
Sbjct: 421 RG--INSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYIS 480

Query: 485 RSS------NPKARVNFHKTIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILAS 544
            SS      +P A + F  T +G +PAP VA +S+RGP+   P +LKPD++APG  ILA+
Sbjct: 481 ESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAA 540

Query: 545 WPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTAD 604
           WP  +  + V S    ++FN+LSGTSMACPH +G+AALLK AHP WSPAAIRSA++TTA 
Sbjct: 541 WPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAY 600

Query: 605 IVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKN 664
            VDN+   + D    N  ++ +  GSGHV+P KA+DPGL+YD+   DY N LC  NYT+ 
Sbjct: 601 TVDNSGEPMMDESTGN-TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRT 660

Query: 665 QIQTITRSTSNHCEKA-----LLDLNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEK 724
            I TITR  ++ C+ A     + +LNYPSF +       Q G ++MS  F R VTN+G+ 
Sbjct: 661 NIVTITRRQAD-CDGARRAGHVGNLNYPSFSVVF----QQYGESKMSTHFIRTVTNVGDS 720

Query: 725 GATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGHVSIKRE---SDVVFGYLNW 729
            + Y  K+ P +G  VTVEP KL F+R  Q LSF ++++    +K     ++V  G++ W
Sbjct: 721 DSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK-TTEVKLSPGATNVETGHIVW 771

BLAST of CmoCh05G001430 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.9e-131
Identity = 296/778 (38.05%), Postives = 424/778 (54.50%), Query Frame = 0

Query: 8   FFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRTTT 67
           FF +  +      S+ +    YIVHM  + MP  F  H +WY ++L S+           
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI----------- 70

Query: 68  SSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSNFL 127
                S  ++L++ Y++AI+GF+  LT  + D+L   PG +S L +      TT +  FL
Sbjct: 71  -----SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFL 130

Query: 128 ALSPNSG-LLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNAS 187
            L  ++  L P +   SDV++G +DTGVWPES+S++D+G   IPS WKG CE+GT+F AS
Sbjct: 131 GLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 190

Query: 188 LCNKKLIG---------------------------------------GSYVKEASFFGYG 247
           LCN+KLIG                                       GS V+ AS  GY 
Sbjct: 191 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 250

Query: 248 QGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 307
            GTARG+APRARVA+YK  W  G   SD++AAID+AI+D V+V+S+SLG      Y D V
Sbjct: 251 SGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGV 310

Query: 308 AIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVL 367
           AI  FAAMERGI V+ SAGN GP   ++ + APW+  V AGT+DRDF     L NG +  
Sbjct: 311 AIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 370

Query: 368 GSSLFPLNSAIALSPLPIVFMGQCHNLKK----------LKRVGFKIVVCEDSDEYSLDL 427
           G SLF    A+    LP ++ G   N              ++V  KIV+C+     +  +
Sbjct: 371 GVSLFK-GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRG--INARV 430

Query: 428 QVDNVESAKIAVGVFISN-ISDWDNLIQTS--FPSIFLNAYHGNVIKDYIKRSSNPKARV 487
           Q  +V  A   VG+ ++N  ++ + L+  +   P+  +    G++I+ Y+    NP A +
Sbjct: 431 QKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASI 490

Query: 488 NFHKTIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPI 547
           +   T++G KP+P VA +SSRGP+   P +LKPD++APG  ILA+W      T + S   
Sbjct: 491 SILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR 550

Query: 548 YSKFNVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNK 607
             +FN++SGTSM+CPH +G+AALLK  HP WSPAAIRSA+MTTA         + D+   
Sbjct: 551 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT- 610

Query: 608 NKVATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEK 667
            K +TP   G+GHV+P  A +PGLIYD+  EDY   LCALNYT  QI++++R     C+ 
Sbjct: 611 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYT-CDP 670

Query: 668 ----ALLDLNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMT-PMKGLV 727
               ++ DLNYPSF + V+   +         ++ R VT++G  G TY  K+T    G+ 
Sbjct: 671 SKSYSVADLNYPSFAVNVDGVGAY--------KYTRTVTSVGGAG-TYSVKVTSETTGVK 730

BLAST of CmoCh05G001430 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 1.0e-128
Identity = 280/771 (36.32%), Postives = 415/771 (53.83%), Query Frame = 0

Query: 11  VSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRTTTSSS 70
           ++  +  L  +T  +T  YI+ ++ +  P+ F THH WY++ L+S               
Sbjct: 14  ITTFLFLLLHTTAKKT--YIIRVNHSDKPESFLTHHDWYTSQLNS--------------- 73

Query: 71  SSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSL-RDSSVRADTTHSSNFLAL 130
                S L++ Y  + +GF+A L  ++ D+L +S   +  +  D      TT +  FL L
Sbjct: 74  ----ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL 133

Query: 131 SPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCN 190
           +   G+  + +  + VIIG +DTGVWPES SF+D  + +IPS+WKGECESG+ F++ LCN
Sbjct: 134 NSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCN 193

Query: 191 KKLIG----------------------------------------GSYVKEASFFGYGQG 250
           KKLIG                                        GS V+ ASF GY  G
Sbjct: 194 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 253

Query: 251 TARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAI 310
           TARG+A RARVA YK  W  G   SD++AA+D+AI DGVDV+SLSLG    P Y D +AI
Sbjct: 254 TARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAI 313

Query: 311 ATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGS 370
             F+AMERG+FV+ SAGN GP   +V + APWV+ V AGT+DRDF     L NG  + G 
Sbjct: 314 GAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGV 373

Query: 371 SLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGF--------KIVVCEDSDEYSLDLQVDN 430
           SL+     +   PL +V+     +   L   G         KIVVC    +  ++ +V+ 
Sbjct: 374 SLYS-GVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVC----DRGVNARVEK 433

Query: 431 VESAKIAVG---VFISNISDWDNLIQTS--FPSIFLNAYHGNVIKDYIKRSSNPKARVNF 490
               + A G   +  +  +  + L+  S   P+I +    G+++++Y+K  S P A + F
Sbjct: 434 GAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVF 493

Query: 491 HKTIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYS 550
             T++  KP+P VA +SSRGP+   P +LKPD++ PG  ILA W   +  T +      +
Sbjct: 494 KGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRT 553

Query: 551 KFNVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNK 610
           +FN++SGTSM+CPH +G+A LLK AHP WSP+AI+SA+MTTA ++DNT   + D  + N 
Sbjct: 554 QFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NS 613

Query: 611 VATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKAL 670
           ++ P A GSGHV+P KA+ PGL+YD+  E+Y   LC+L+YT + I  I +  S +C K  
Sbjct: 614 LSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKF 673

Query: 671 LD---LNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVE 725
            D   LNYPSF +         G   + R + R VTN+G   + Y+  +     + ++V+
Sbjct: 674 SDPGQLNYPSFSVLF-------GGKRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVK 733

BLAST of CmoCh05G001430 vs. ExPASy TrEMBL
Match: A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)

HSP 1 Score: 1426.4 bits (3691), Expect = 0.0e+00
Identity = 732/769 (95.19%), Postives = 732/769 (95.19%), Query Frame = 0

Query: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
           MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT
Sbjct: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60

Query: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
           SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT
Sbjct: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120

Query: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
           THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180

Query: 181 THFNASLCNKKLIG-------------------------------------GSYVKEASF 240
           THFNASLCNKKLIG                                     GSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240

Query: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
           FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300

Query: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
           EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360

Query: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
           VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE
Sbjct: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420

Query: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
           SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480

Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
           KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540

Query: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
           TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600

Query: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
           GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660

Query: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
           FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN
Sbjct: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720

Query: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL 733
           QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL
Sbjct: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL 769

BLAST of CmoCh05G001430 vs. ExPASy TrEMBL
Match: A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)

HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 695/764 (90.97%), Postives = 709/764 (92.80%), Query Frame = 0

Query: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
           MAAHMRMFF +SLMVI LARSTLT+TDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT
Sbjct: 1   MAAHMRMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60

Query: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
           SPLRTTT SSSSSLPSKLIHCYKHAINGFTA+LTPSQLDALKNSPGYVSS+RDSSVRADT
Sbjct: 61  SPLRTTT-SSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120

Query: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
           THSSNFLAL+PNSGLLPISNYGSDVIIGFVDTGVWPESESFND+ ISKIPSRWKGECESG
Sbjct: 121 THSSNFLALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESG 180

Query: 181 THFNASLCNKKLIG-------------------------------------GSYVKEASF 240
           THFNASLCNKKLIG                                     GSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240

Query: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
           FGYGQGTARGVAPRARVAIYKAIWKEGN VSDVIAAIDQAISDGVDVISLSLGLDGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300

Query: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
           EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITL NG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNG 360

Query: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
           VSVLGSSLF LNSAI LSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNV+
Sbjct: 361 VSVLGSSLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQ 420

Query: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
           SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYI RSSNPKARVNFHKTIIGT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGT 480

Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
           KPAPSVARYSSRGPSESCP VLKPDIMAPGDAILASWPQ VAATDVYSRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSG 540

Query: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
           TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTAD+VDNTQTLIKDLGNKNKVATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAM 600

Query: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
           GSGHVNPNKAIDPGLIYD+GIEDYTNLLCALNYTKNQIQTITRS SN CEK  LDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPS 660

Query: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
           FI+TVNASDS++GR E+SREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEP  LKFKRKN
Sbjct: 661 FIITVNASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKN 720

Query: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA 728
           QNLSFKLKIRGHVS+KRESDVVFGYLNWVEVGGGHRVQSPIVVA
Sbjct: 721 QNLSFKLKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVA 763

BLAST of CmoCh05G001430 vs. ExPASy TrEMBL
Match: A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 559/760 (73.55%), Postives = 632/760 (83.16%), Query Frame = 0

Query: 5   MRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLR 64
           +R++FW SL  IF     LT+T NYIVHM+ AAMPKPFA+ HSWYSAT+SSVL  +S   
Sbjct: 3   VRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSS 62

Query: 65  TTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSS 124
           +++SSSSSS PSKLIH Y HAI+GF ASLTPSQL+ALKNSPGY+SS+ DSSV  DTTHSS
Sbjct: 63  SSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSS 122

Query: 125 NFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFN 184
           +FL LS N GLLPIS YGSDVIIGFVDTG+WP+SESFNDDG+S+IPSRWKGECES THFN
Sbjct: 123 HFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFN 182

Query: 185 ASLCNKKLIG-------------------------------------GSYVKEASFFGYG 244
            S CN KLIG                                     GSYVKEASFFGYG
Sbjct: 183 VSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYG 242

Query: 245 QGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 304
           +GTARGVAPRARVAIYKAIW EGN+VSDV+AAIDQAISDGVDVISLS+G+DG+PLY DPV
Sbjct: 243 RGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPV 302

Query: 305 AIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVL 364
           +IATFAA+ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL+NGVSVL
Sbjct: 303 SIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVL 362

Query: 365 GSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKI 424
           GSSLFPLN+ + LSPLPIVFMG C NLKKLKR+G+KIVVCED+D YSL  QVDNV++AK+
Sbjct: 363 GSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKV 422

Query: 425 AVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAP 484
            +G+FISNI DWDNLIQT FPSIF+N YHGN+IKDYI +SS+PKA VNFHKTI+GTKPAP
Sbjct: 423 TLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAP 482

Query: 485 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMA 544
           +VARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQN+ A DV S PIY+KFNV+SGTSM+
Sbjct: 483 TVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMS 542

Query: 545 CPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGH 604
           CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD+VDNTQT IKD+GN NK ATPLAMGSGH
Sbjct: 543 CPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGH 602

Query: 605 VNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMT 664
           VNPNKA+DP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE   LDLNYPSFI+ 
Sbjct: 603 VNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIII 662

Query: 665 VNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLS 724
            N+SDS+TG+ ++  EFKR +T IGE  ATY AK+  MKG  V V+P  L+FKRKNQ LS
Sbjct: 663 ANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLS 722

Query: 725 FKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA 728
           F+LKI G      ES+VVFGYL+W EVGG H VQSPIVVA
Sbjct: 723 FELKIAGSA---EESNVVFGYLSWAEVGGRHIVQSPIVVA 759

BLAST of CmoCh05G001430 vs. ExPASy TrEMBL
Match: A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 559/760 (73.55%), Postives = 632/760 (83.16%), Query Frame = 0

Query: 5   MRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLR 64
           +R++FW SL  IF     LT+T NYIVHM+ AAMPKPFA+ HSWYSAT+SSVL  +S   
Sbjct: 3   VRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSS 62

Query: 65  TTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSS 124
           +++SSSSSS PSKLIH Y HAI+GF ASLTPSQL+ALKNSPGY+SS+ DSSV  DTTHSS
Sbjct: 63  SSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSS 122

Query: 125 NFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFN 184
           +FL LS N GLLPIS YGSDVIIGFVDTG+WP+SESFNDDG+S+IPSRWKGECES THFN
Sbjct: 123 HFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFN 182

Query: 185 ASLCNKKLIG-------------------------------------GSYVKEASFFGYG 244
            S CN KLIG                                     GSYVKEASFFGYG
Sbjct: 183 VSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYG 242

Query: 245 QGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 304
           +GTARGVAPRARVAIYKAIW EGN+VSDV+AAIDQAISDGVDVISLS+G+DG+PLY DPV
Sbjct: 243 RGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPV 302

Query: 305 AIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVL 364
           +IATFAA+ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL+NGVSVL
Sbjct: 303 SIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVL 362

Query: 365 GSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKI 424
           GSSLFPLN+ + LSPLPIVFMG C NLKKLKR+G+KIVVCED+D YSL  QVDNV++AK+
Sbjct: 363 GSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKV 422

Query: 425 AVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAP 484
            +G+FISNI DWDNLIQT FPSIF+N YHGN+IKDYI +SS+PKA VNFHKTI+GTKPAP
Sbjct: 423 TLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAP 482

Query: 485 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMA 544
           +VARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQN+ A DV S PIY+KFNV+SGTSM+
Sbjct: 483 TVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMS 542

Query: 545 CPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGH 604
           CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD+VDNTQT IKD+GN NK ATPLAMGSGH
Sbjct: 543 CPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGH 602

Query: 605 VNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMT 664
           VNPNKA+DP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE   LDLNYPSFI+ 
Sbjct: 603 VNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIII 662

Query: 665 VNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLS 724
            N+SDS+TG+ ++  EFKR +T IGE  ATY AK+  MKG  V V+P  L+FKRKNQ LS
Sbjct: 663 ANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLS 722

Query: 725 FKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA 728
           F+LKI G      ES+VVFGYL+W EVGG H VQSPIVVA
Sbjct: 723 FELKIAGSA---EESNVVFGYLSWAEVGGRHIVQSPIVVA 759

BLAST of CmoCh05G001430 vs. ExPASy TrEMBL
Match: A0A0A0LKL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354760 PE=3 SV=1)

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 559/759 (73.65%), Postives = 626/759 (82.48%), Query Frame = 0

Query: 6   RMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65
           R++FW SL+ IF     LT+T NYIVHM+ AAMPKPFA+ HSWYSAT+SS+L        
Sbjct: 4   RLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLL-------H 63

Query: 66  TTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSN 125
           ++SSSSSS PSKLIH Y HAI+GF ASLTPSQL+ALKNSPGY+SS+ DSSV  DTTHSS+
Sbjct: 64  SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSH 123

Query: 126 FLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNA 185
           FL LS N GLLPIS YGSDVIIGFVDTG+WP+SESF DDG+S+IPS+WKGECES THFN 
Sbjct: 124 FLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNV 183

Query: 186 SLCNKKLIG-------------------------------------GSYVKEASFFGYGQ 245
           S CN KLIG                                     GSY+KEASFFGYG+
Sbjct: 184 SFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGR 243

Query: 246 GTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVA 305
           GTARGVAPRARVAIYKAIW+EGN+VSDV+AAIDQAISDGVDVISLS+G+DGVPLY+DPVA
Sbjct: 244 GTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVA 303

Query: 306 IATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLG 365
           IATFAA+ERGIFVATSAGN GPQ  TVH+GAPW+LNVAAGTMDRDFGGTITL+NGVSVLG
Sbjct: 304 IATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLG 363

Query: 366 SSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIA 425
           SSLFPLN    LSPLPIVFMG C NLKKL+R G+KIVVCEDSD YSL  QVDNV++A +A
Sbjct: 364 SSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVA 423

Query: 426 VGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPS 485
           +G+FISNI DWDNLIQT FPSIFLN YHGN+IKDYI +SS+PKA V FHKTI+ TKPAP 
Sbjct: 424 LGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPM 483

Query: 486 VARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMAC 545
           VARYSSRGPS+SCPFVLKPDIMAPGD ILASWPQNV A DV S PIYSKFNV+SGTSM+C
Sbjct: 484 VARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSC 543

Query: 546 PHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHV 605
           PHAAGVAALLKGAHP WSPAAIRSAMMTTADI+DNTQT IKD GN NK ATPLAMGSGHV
Sbjct: 544 PHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHV 603

Query: 606 NPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTV 665
           NPNKAIDP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE   LDLNYPSFIM V
Sbjct: 604 NPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIV 663

Query: 666 NASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSF 725
           N+SDS+T + ++S EFKR +T IGE  ATY AK+T MKG  V V+PNKL FKRKNQ LSF
Sbjct: 664 NSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSF 723

Query: 726 KLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA 728
           +LKI G     RES++VFGYL+W EVGGGH +QSPIVV+
Sbjct: 724 ELKIAGSA---RESNIVFGYLSWAEVGGGHIIQSPIVVS 752

BLAST of CmoCh05G001430 vs. NCBI nr
Match: XP_022962414.1 (subtilisin-like protease SBT1.9 [Cucurbita moschata])

HSP 1 Score: 1426.4 bits (3691), Expect = 0.0e+00
Identity = 732/769 (95.19%), Postives = 732/769 (95.19%), Query Frame = 0

Query: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
           MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT
Sbjct: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60

Query: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
           SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT
Sbjct: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120

Query: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
           THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180

Query: 181 THFNASLCNKKLIG-------------------------------------GSYVKEASF 240
           THFNASLCNKKLIG                                     GSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240

Query: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
           FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300

Query: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
           EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360

Query: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
           VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE
Sbjct: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420

Query: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
           SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480

Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
           KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540

Query: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
           TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600

Query: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
           GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660

Query: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
           FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN
Sbjct: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720

Query: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL 733
           QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL
Sbjct: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL 769

BLAST of CmoCh05G001430 vs. NCBI nr
Match: KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1405.6 bits (3637), Expect = 0.0e+00
Identity = 721/769 (93.76%), Postives = 728/769 (94.67%), Query Frame = 0

Query: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
           MAAHMRMFFWVSL+VIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT
Sbjct: 1   MAAHMRMFFWVSLIVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60

Query: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
           SPLRTTTSSSSSS PSKLIHCYKHAINGFTA+LTPSQLDALKNSPGYVSS+RDSSVRADT
Sbjct: 61  SPLRTTTSSSSSS-PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120

Query: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
           THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGI KIPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKGECESG 180

Query: 181 THFNASLCNKKLIG-------------------------------------GSYVKEASF 240
           THFNASLCNKKLIG                                     GSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240

Query: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
           FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300

Query: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
           EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360

Query: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
           VSVLGSSLFPLN AIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE
Sbjct: 361 VSVLGSSLFPLNLAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420

Query: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
           SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480

Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
           KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSK+NVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSG 540

Query: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
           TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600

Query: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
           GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEK LLDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPS 660

Query: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
           FI+TVNASDSQTGRTE+SREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN
Sbjct: 661 FIITVNASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720

Query: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL 733
           QNLSFKLKIRGHVS+KRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL
Sbjct: 721 QNLSFKLKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL 768

BLAST of CmoCh05G001430 vs. NCBI nr
Match: KAG7029306.1 (Subtilisin-like protease SBT1.9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 721/769 (93.76%), Postives = 727/769 (94.54%), Query Frame = 0

Query: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
           MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMP  FATHHSWYSATLSSVLLDT
Sbjct: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPNSFATHHSWYSATLSSVLLDT 60

Query: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
           SPLRTTTSSSSSS PSKLIHCYKHAINGFTA+LTPSQLDALKNSPGYVSS+RDSSVRADT
Sbjct: 61  SPLRTTTSSSSSS-PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120

Query: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
           THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180

Query: 181 THFNASLCNKKLIG-------------------------------------GSYVKEASF 240
           THFNASLCNKKLIG                                     GSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240

Query: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
           FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300

Query: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
           EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360

Query: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
           VSVLGSSLFPLN AIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE
Sbjct: 361 VSVLGSSLFPLNLAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420

Query: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
           SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480

Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
           KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSK+NVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSG 540

Query: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
           TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600

Query: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
           GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEK LLDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPS 660

Query: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
           FI+TVNASDSQTGRTE+SREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN
Sbjct: 661 FIITVNASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720

Query: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL 733
           QNLSFKLKIRGHVS+KRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL
Sbjct: 721 QNLSFKLKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGL 768

BLAST of CmoCh05G001430 vs. NCBI nr
Match: XP_023546329.1 (subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1377.8 bits (3565), Expect = 0.0e+00
Identity = 706/776 (90.98%), Postives = 722/776 (93.04%), Query Frame = 0

Query: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
           MAAHMRMFFW+SLMVIFLARST+T+TDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT
Sbjct: 1   MAAHMRMFFWISLMVIFLARSTVTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60

Query: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
           SPLRTTT SSSSSLPSKLIHCYKHAINGFTA+LTPSQLDALKNSPGYVSS+RDSSVRADT
Sbjct: 61  SPLRTTT-SSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120

Query: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
           THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180

Query: 181 THFNASLCNKKLIG-------------------------------------GSYVKEASF 240
           THFNASLCNKKLIG                                     GSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240

Query: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
           FGYGQGTARGVAPRARVAIYKAIWKEGN VSDVIAAIDQAISDGVDVI+LSLG+D VPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVIALSLGIDRVPLY 300

Query: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
           EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360

Query: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
           VSVLGSSLFPLNSAIALSPLPI FMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNV+
Sbjct: 361 VSVLGSSLFPLNSAIALSPLPIFFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQ 420

Query: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
           SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNV+KDYI RSSNPKARVNFHKTIIGT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVLKDYINRSSNPKARVNFHKTIIGT 480

Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
           KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540

Query: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
           TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600

Query: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
           GSGHVNPNKAIDPGLIYDMGIEDYTNL+CALNYTKNQIQTITRSTSN CEK  LDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLICALNYTKNQIQTITRSTSNDCEKPSLDLNYPS 660

Query: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
           FI+TVNASDS+TGRTE+SREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEP KLKFKRKN
Sbjct: 661 FIITVNASDSETGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKKLKFKRKN 720

Query: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGLSPLRPPS 740
           QNLSFKLKIRGHVS+KRESDVVFGYLNWVEVGGGHRVQSPI   VV  +S  R P+
Sbjct: 721 QNLSFKLKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPI---VVTNMSSSRTPT 772

BLAST of CmoCh05G001430 vs. NCBI nr
Match: XP_022996869.1 (subtilisin-like protease SBT1.9 [Cucurbita maxima])

HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 695/764 (90.97%), Postives = 709/764 (92.80%), Query Frame = 0

Query: 1   MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
           MAAHMRMFF +SLMVI LARSTLT+TDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT
Sbjct: 1   MAAHMRMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60

Query: 61  SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
           SPLRTTT SSSSSLPSKLIHCYKHAINGFTA+LTPSQLDALKNSPGYVSS+RDSSVRADT
Sbjct: 61  SPLRTTT-SSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120

Query: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
           THSSNFLAL+PNSGLLPISNYGSDVIIGFVDTGVWPESESFND+ ISKIPSRWKGECESG
Sbjct: 121 THSSNFLALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESG 180

Query: 181 THFNASLCNKKLIG-------------------------------------GSYVKEASF 240
           THFNASLCNKKLIG                                     GSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240

Query: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
           FGYGQGTARGVAPRARVAIYKAIWKEGN VSDVIAAIDQAISDGVDVISLSLGLDGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300

Query: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
           EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITL NG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNG 360

Query: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
           VSVLGSSLF LNSAI LSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNV+
Sbjct: 361 VSVLGSSLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQ 420

Query: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
           SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYI RSSNPKARVNFHKTIIGT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGT 480

Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
           KPAPSVARYSSRGPSESCP VLKPDIMAPGDAILASWPQ VAATDVYSRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSG 540

Query: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
           TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTAD+VDNTQTLIKDLGNKNKVATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAM 600

Query: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
           GSGHVNPNKAIDPGLIYD+GIEDYTNLLCALNYTKNQIQTITRS SN CEK  LDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPS 660

Query: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
           FI+TVNASDS++GR E+SREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEP  LKFKRKN
Sbjct: 661 FIITVNASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKN 720

Query: 721 QNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA 728
           QNLSFKLKIRGHVS+KRESDVVFGYLNWVEVGGGHRVQSPIVVA
Sbjct: 721 QNLSFKLKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVA 763

BLAST of CmoCh05G001430 vs. TAIR 10
Match: AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 547.0 bits (1408), Expect = 3.9e-155
Identity = 316/762 (41.47%), Postives = 441/762 (57.87%), Query Frame = 0

Query: 5   MRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLR 64
           M M   + L+  F       +T  YI+HMDL+A P PF+ H SW+S TL+SV+ +  P  
Sbjct: 1   MGMTVVIILVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRKP-- 60

Query: 65  TTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSS 124
                       K+I+ Y  +++GF+A LT S+L  LK+ PGYVS  +D  V+  TT S 
Sbjct: 61  ------------KIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSP 120

Query: 125 NFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFN 184
            F+ L+  SG  P+SNYG+ ++IG +DTG+WP+S SF+DDG+  +PS+WKG CE     +
Sbjct: 121 KFIGLNSTSGTWPVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---S 180

Query: 185 ASLCNKKLIG-----------------------------------------GSYVKEASF 244
           +SLCNKKLIG                                         G++VK AS+
Sbjct: 181 SSLCNKKLIGAKVFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASY 240

Query: 245 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGL------ 304
           F Y QGTA G+AP A +AIYKA W+EG   SDVIAAIDQAI DGV VISLSLGL      
Sbjct: 241 FSYAQGTASGIAPHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDD 300

Query: 305 --DGVPLYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFG 364
             DG  L  DP+A+A+FAA+++G+FV TS GN GP + ++ +GAPW++ V AGT+ R F 
Sbjct: 301 DNDGFGLENDPIAVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQ 360

Query: 365 GTITLTNGVSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSL 424
           GT+T  N VS    SLFP        P+  +  G   N    K +  +IVVC  ++  ++
Sbjct: 361 GTLTFGNRVSFSFPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINI 420

Query: 425 DLQVDNVESAKIAVGVFISN-ISDWDNLIQTSFPSIFLNAYHGNVIKDYI-KRSSNPKAR 484
             ++  + S   A  V I++ + +  + I+  FP  F+ + H   I+ Y     +N  A+
Sbjct: 421 GSKLHQIRSTGAAAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAK 480

Query: 485 VNFHKTIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRP 544
           + F KT+IGTKPAP V  YSSRGP  S P +LKPDI+APG  IL++WP     T   + P
Sbjct: 481 LEFRKTVIGTKPAPEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALP 540

Query: 545 IYSKFNVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGN 604
           ++S FN+L+GTSMA PH AGVAAL+K  HP WSP+AI+SA+MTTA  +DN          
Sbjct: 541 LFSGFNLLTGTSMAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN---------- 600

Query: 605 KNKVATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLC-ALNYTKNQIQTITRST-SNH 664
                 PLA+G+GHV+ NK ++PGLIYD   +D+ N LC     ++  I  ITRS  S+ 
Sbjct: 601 ------PLAVGAGHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDA 660

Query: 665 CEKALLDLNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVT 714
           C+K    LNYPS I    +  S        + FKR +TN+GE   +Y  ++  +KGL V 
Sbjct: 661 CKKPSPYLNYPSIIAYFTSDQSS------PKIFKRTLTNVGEAKRSYIVRVRGLKGLNVV 715

BLAST of CmoCh05G001430 vs. TAIR 10
Match: AT3G01400.1 (ARM repeat superfamily protein )

HSP 1 Score: 487.6 bits (1254), Expect = 2.8e-137
Identity = 260/330 (78.79%), Postives = 296/330 (89.70%), Query Frame = 0

Query: 816  LEMGTENPVNFSYMGRSFDDLTNGDSSGAFSDCNSDRSGEFPMASSQSRRLLISCASDNS 875
            +EM    P +F+YMGR F DL+  D S AFSDCNSDRSGEFP ASS+SRRLL+SCAS+NS
Sbjct: 1    MEMENHRPGSFTYMGRKFSDLSLNDDSSAFSDCNSDRSGEFPTASSESRRLLLSCASENS 60

Query: 876  DELIQQLVLDLESC-SIDEQKQAAMEIRLLAKNKPENRLRIAKAGAVRPLISLISCTDPQ 935
            D+LI  LV  L+S  SIDEQKQAAMEIRLL+KNKPENR++IAKAGA++PLISLIS +D Q
Sbjct: 61   DDLINHLVSHLDSSYSIDEQKQAAMEIRLLSKNKPENRIKIAKAGAIKPLISLISSSDLQ 120

Query: 936  LQEYGVTAILNLSLNDENKELIAASGAIKPLVRALMSGTSTAKENAACALLRLSQMEENK 995
            LQEYGVTAILNLSL DENKE IA+SGAIKPLVRAL  GT TAKENAACALLRLSQ+EENK
Sbjct: 121  LQEYGVTAILNLSLCDENKESIASSGAIKPLVRALKMGTPTAKENAACALLRLSQIEENK 180

Query: 996  IAIGRSGAIPLLVNLLGNGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADF 1055
            +AIGRSGAIPLLVNLL  GGFR KKDASTALYSLCS KENKIRAV++GIM+PLVELMADF
Sbjct: 181  VAIGRSGAIPLLVNLLETGGFRAKKDASTALYSLCSAKENKIRAVQSGIMKPLVELMADF 240

Query: 1056 GSNMVDKSAFVLSVLVSMSEARTALVDEGGIPVLVELVEDGTQRQKEIAAVILLQICEES 1115
            GSNMVDKSAFV+S+L+S+ E++ A+V+EGG+PVLVE+VE GTQRQKE+A  ILLQ+CEES
Sbjct: 241  GSNMVDKSAFVMSLLMSVPESKPAIVEEGGVPVLVEIVEVGTQRQKEMAVSILLQLCEES 300

Query: 1116 VLYRTMVAREGAIPPLVALSQSGTNRAKQK 1145
            V+YRTMVAREGAIPPLVALSQ+GT+RAKQK
Sbjct: 301  VVYRTMVAREGAIPPLVALSQAGTSRAKQK 330

BLAST of CmoCh05G001430 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 472.2 bits (1214), Expect = 1.2e-132
Identity = 302/797 (37.89%), Postives = 428/797 (53.70%), Query Frame = 0

Query: 5   MRMFFWVSLMVIFLARSTLTQTDN---YIVHMDLAAMPKPFATHHSWYSATLSSVLLDTS 64
           M  FF+   ++   + S+   + N   YIVH+D  A P  F TH  WY+++L+S+     
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----- 60

Query: 65  PLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTT 124
                     +S P  +IH Y    +GF+A LT      L + P  +S + +      TT
Sbjct: 61  ----------TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTT 120

Query: 125 HSSNFLAL--SPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECES 184
            S  FL L  +  +GLL  S++GSD++IG +DTGVWPE  SF+D G+  +P +WKG+C +
Sbjct: 121 RSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIA 180

Query: 185 GTHFNASLCNKKLIG---------------------------------------GSYVKE 244
              F  S CN+KL+G                                       G YV  
Sbjct: 181 SQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFP 240

Query: 245 ASFFGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGV 304
           AS  GY  G A G+AP+AR+A YK  W  G   SD++AA D A++DGVDVISLS+G   V
Sbjct: 241 ASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVV 300

Query: 305 PLYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITL 364
           P Y D +AI  F A++RGIFV+ SAGN GP   TV + APW+  V AGT+DRDF   + L
Sbjct: 301 PYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKL 360

Query: 365 TNGVSVLGSSLFPLNSAIALSPLPIVFMGQ------------CHNLKKLKRVGFKIVVCE 424
            NG  + G S++           P+V+ G                      V  KIV+C+
Sbjct: 361 GNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCD 420

Query: 425 DSDEYSLDLQVDNVESAKIAVGVFISN-ISDWDNLIQTS--FPSIFLNAYHGNVIKDYIK 484
                +       +      +G+ I+N + D + L+      P+  + A  G+ I+ YI 
Sbjct: 421 RG--INSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYIS 480

Query: 485 RSS------NPKARVNFHKTIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILAS 544
            SS      +P A + F  T +G +PAP VA +S+RGP+   P +LKPD++APG  ILA+
Sbjct: 481 ESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAA 540

Query: 545 WPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTAD 604
           WP  +  + V S    ++FN+LSGTSMACPH +G+AALLK AHP WSPAAIRSA++TTA 
Sbjct: 541 WPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAY 600

Query: 605 IVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKN 664
            VDN+   + D    N  ++ +  GSGHV+P KA+DPGL+YD+   DY N LC  NYT+ 
Sbjct: 601 TVDNSGEPMMDESTGN-TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRT 660

Query: 665 QIQTITRSTSNHCEKA-----LLDLNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEK 724
            I TITR  ++ C+ A     + +LNYPSF +       Q G ++MS  F R VTN+G+ 
Sbjct: 661 NIVTITRRQAD-CDGARRAGHVGNLNYPSFSVVF----QQYGESKMSTHFIRTVTNVGDS 720

Query: 725 GATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGHVSIKRE---SDVVFGYLNW 729
            + Y  K+ P +G  VTVEP KL F+R  Q LSF ++++    +K     ++V  G++ W
Sbjct: 721 DSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK-TTEVKLSPGATNVETGHIVW 771

BLAST of CmoCh05G001430 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 471.5 bits (1212), Expect = 2.1e-132
Identity = 296/778 (38.05%), Postives = 424/778 (54.50%), Query Frame = 0

Query: 8   FFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRTTT 67
           FF +  +      S+ +    YIVHM  + MP  F  H +WY ++L S+           
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI----------- 70

Query: 68  SSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSNFL 127
                S  ++L++ Y++AI+GF+  LT  + D+L   PG +S L +      TT +  FL
Sbjct: 71  -----SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFL 130

Query: 128 ALSPNSG-LLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNAS 187
            L  ++  L P +   SDV++G +DTGVWPES+S++D+G   IPS WKG CE+GT+F AS
Sbjct: 131 GLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 190

Query: 188 LCNKKLIG---------------------------------------GSYVKEASFFGYG 247
           LCN+KLIG                                       GS V+ AS  GY 
Sbjct: 191 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 250

Query: 248 QGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 307
            GTARG+APRARVA+YK  W  G   SD++AAID+AI+D V+V+S+SLG      Y D V
Sbjct: 251 SGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGV 310

Query: 308 AIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVL 367
           AI  FAAMERGI V+ SAGN GP   ++ + APW+  V AGT+DRDF     L NG +  
Sbjct: 311 AIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 370

Query: 368 GSSLFPLNSAIALSPLPIVFMGQCHNLKK----------LKRVGFKIVVCEDSDEYSLDL 427
           G SLF    A+    LP ++ G   N              ++V  KIV+C+     +  +
Sbjct: 371 GVSLFK-GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRG--INARV 430

Query: 428 QVDNVESAKIAVGVFISN-ISDWDNLIQTS--FPSIFLNAYHGNVIKDYIKRSSNPKARV 487
           Q  +V  A   VG+ ++N  ++ + L+  +   P+  +    G++I+ Y+    NP A +
Sbjct: 431 QKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASI 490

Query: 488 NFHKTIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPI 547
           +   T++G KP+P VA +SSRGP+   P +LKPD++APG  ILA+W      T + S   
Sbjct: 491 SILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR 550

Query: 548 YSKFNVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNK 607
             +FN++SGTSM+CPH +G+AALLK  HP WSPAAIRSA+MTTA         + D+   
Sbjct: 551 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT- 610

Query: 608 NKVATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEK 667
            K +TP   G+GHV+P  A +PGLIYD+  EDY   LCALNYT  QI++++R     C+ 
Sbjct: 611 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYT-CDP 670

Query: 668 ----ALLDLNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMT-PMKGLV 727
               ++ DLNYPSF + V+   +         ++ R VT++G  G TY  K+T    G+ 
Sbjct: 671 SKSYSVADLNYPSFAVNVDGVGAY--------KYTRTVTSVGGAG-TYSVKVTSETTGVK 730

BLAST of CmoCh05G001430 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 463.0 bits (1190), Expect = 7.4e-130
Identity = 280/771 (36.32%), Postives = 415/771 (53.83%), Query Frame = 0

Query: 11  VSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRTTTSSS 70
           ++  +  L  +T  +T  YI+ ++ +  P+ F THH WY++ L+S               
Sbjct: 14  ITTFLFLLLHTTAKKT--YIIRVNHSDKPESFLTHHDWYTSQLNS--------------- 73

Query: 71  SSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSL-RDSSVRADTTHSSNFLAL 130
                S L++ Y  + +GF+A L  ++ D+L +S   +  +  D      TT +  FL L
Sbjct: 74  ----ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL 133

Query: 131 SPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCN 190
           +   G+  + +  + VIIG +DTGVWPES SF+D  + +IPS+WKGECESG+ F++ LCN
Sbjct: 134 NSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCN 193

Query: 191 KKLIG----------------------------------------GSYVKEASFFGYGQG 250
           KKLIG                                        GS V+ ASF GY  G
Sbjct: 194 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 253

Query: 251 TARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAI 310
           TARG+A RARVA YK  W  G   SD++AA+D+AI DGVDV+SLSLG    P Y D +AI
Sbjct: 254 TARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAI 313

Query: 311 ATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGS 370
             F+AMERG+FV+ SAGN GP   +V + APWV+ V AGT+DRDF     L NG  + G 
Sbjct: 314 GAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGV 373

Query: 371 SLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGF--------KIVVCEDSDEYSLDLQVDN 430
           SL+     +   PL +V+     +   L   G         KIVVC    +  ++ +V+ 
Sbjct: 374 SLYS-GVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVC----DRGVNARVEK 433

Query: 431 VESAKIAVG---VFISNISDWDNLIQTS--FPSIFLNAYHGNVIKDYIKRSSNPKARVNF 490
               + A G   +  +  +  + L+  S   P+I +    G+++++Y+K  S P A + F
Sbjct: 434 GAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVF 493

Query: 491 HKTIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYS 550
             T++  KP+P VA +SSRGP+   P +LKPD++ PG  ILA W   +  T +      +
Sbjct: 494 KGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRT 553

Query: 551 KFNVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNK 610
           +FN++SGTSM+CPH +G+A LLK AHP WSP+AI+SA+MTTA ++DNT   + D  + N 
Sbjct: 554 QFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NS 613

Query: 611 VATPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKAL 670
           ++ P A GSGHV+P KA+ PGL+YD+  E+Y   LC+L+YT + I  I +  S +C K  
Sbjct: 614 LSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKF 673

Query: 671 LD---LNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVE 725
            D   LNYPSF +         G   + R + R VTN+G   + Y+  +     + ++V+
Sbjct: 674 SDPGQLNYPSFSVLF-------GGKRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVK 733