CmoCh05G001160 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G001160
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionpiRNA biogenesis protein EXD1-like isoform X2
LocationCmo_Chr05: 481471 .. 487715 (+)
RNA-Seq ExpressionCmoCh05G001160
SyntenyCmoCh05G001160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTGAAAGATTCGCTCAAATGGCGAACACTCCTTCTCGCCAGACGTATATTCCTCTGCCTTCGGACTCAGGTCACTCTCTCTTTCCTCTTTCTTGCGTTCTTGGATTGAACTTCCATTTTCATTCATGATCAATTGCGTATTGTATTCTTTGTCTATCTTTCTTCCTCTTAATGAATGCTTCGTTATTGCAAGTTCGTGGTCTATGGATTGACGTGCGATTCTTAGTAGCAAGTTGTTTTCTCTCTTATCGAAGCTTTAATTGCTGTTTGTTTGGCCAATCAGAAATCGTGGAATATACTTGACTGATGTTGGAAACTGAATTCTGATGCAAGAGAATCACAATGATGTAGTTATCAATGGTTAGAGTAGAAGAATTTGTTTGGAAACTTCAAAAGACATGTTGCTCTAAGTATAGGCCTGTGTTTTCTCTATTTCCATCGTTCTTTCGCCTTTTTACGTCACTTGGATTTAGTTCCAGTTGCTGCGGATAGGTTTGGTTTTCTTGATTGCTTTTGCCGCGCACGGTTGCTTGATTGCTTTACCCGCGCATGTTCCATCAAGTTTTTTAGTTAGCATCTTCATTTTCGCTTCGAGACGATGGAATCTTTCTTGTGGTGGATAGTTTGCGCAATGTTTGATATAAAATAGAACTGGAGATGTTAATTCTCCCCTCGTTCTCCAGCTCCTGTCCTCCATACGACCATTTCGCTCCAAGATTTTTATCATCTTCCAATGTTTTGGTTTTCTTGATTGCTTTTGCCGCACACAGTTGCTTGATTGCTTTACCCGCGCATGCTCCATCAAGTTTTTTAGTTAGTATGTTTATTTTCGCTTCGAGACGATGGAATATTTTCTGTGGTTGATAGTTTGCGCACTGTTTGATATAAAATAGAACTGGAGAGGTTAATTCTCCCATCGTTCCCCAGCTCCTGTCCTTCATACGATCATTTCGCTCCAAGATTTTTATCATCTTCCAATGAGTTCCATTTCATGTGGATTTCAGGTGAAAATCAGAACGATCCTGAAGCCAACAAAACCTTGGTTCCTATTCATATTGTTACTCATGCATCTCAACTCCCTAACGAATTTGTTGATCCATCACCTGAAAAGCCTCTGGTAGTTGGCTTTGATTGTGAAGGTGTCAGCCTGTGTCGCCATGGACGTCTTTGTATCATGCAGGTAAATAAAATGCTAAGAACAAATCGCAGTTTTACCATCCTTGTGGAGAGTGAAATTGTATGGCAGTGGCGGATGTGCTAGCTAACCTTCCCAACTGAGGGAGTCAATGGTTCTAACTTATAATTTCATTCTTTTAGTCATCTGGTGATACTGTCAATTCATAATCCAAGCCGCTTTCTTGCAAGAAAACAACTAATATTATAGCTTAAGAAATTTAATTAGCGCTCCTGGATAGTCCTCGACTTGTAGCTATAGTAACTCATCCTTTAAATTACAGGGGTTGATTACTGTCAAAGGCTTGGACATTCAAGAATGTTCAGTTTCTACTTTACTAAAAAACAGTGATTCAAATTAGTTGACATTATCTATAAATCTGTGCTGTTCATTTCTTGGCAGATTGCATTTCCAGATGCTATATATCTGGTTGATGCTGTTCAGGGAGGAGAGGAACTTGTGAAAGTCTGTAAGCCTGCCCTTGAGTCCACATATGTCACGAAAGTTATTCATGATTGTAAACGAGATAGTGAGGTCTCGTCTGAACTTTGTTTCCGTTACAGATTGCCGTCACAGAGACATGACATGTTTTAGCTAATGTTGACTCTATTTTTTTCCCTGTAGGCATTGTACTTTCAGTTTGGTATTAAGTTGAACAACGTTATTGATACACAGGTGATTATCAATTGTGAGAAGCCTGACACTCTTTACTTGTTTACACCCTTTTCTCATGCATTATATTTTATCTTATTTTAGAGTGCTTAGCCACTTAGTGTGCCAATTTCATGTTACTAGAACATAAGAATATTTTTTGTGCTTTTCTTCTTCTATGTTTTCTTGGATAGAAAATGAAACTATCATATCAGGAAGATGGATTCTACTATTCCACTGATCGTTGTTCCATTATTTCTTATTTAAGTTCCTAGAAGTTTTCATGATGTTAATTTTTCACATCTACTTTGCACTTGTACGATTTGAATTTACAGTCTGCCAGTATTTTTATAAATTTATAATTCTACGTACTCATTTTAAAGGTTTATGTAAATTTTCTCTCAATGAACTCTAATCTTAACTTACTATGGATTATTAACTGATTGAAATCTGCTATCTGATAATTTTTAAGGTTGAAAGTCTGCATGTCTTCAAAAAGAAAAAGAGAGAGAGAGAGAGAGAGAGTGGTTATCTTGTGACATAAATGCCAATAAAGAGTATGATTTTCTTGATTTAAAAGAATGATCTGAACAATTCATTTTAAGTTTGAGCTACCTTGTAATTACTTTGGAGCCAAGTGTTTGTATGTGTAAGCTGCAATATGGAGGAAGGCTGAGTTATTTGGACCTTCCTGTTTTAATTTTCAATTGTATTACTTGATTCAGATTGCATATTCACTTATAGAGGAGCAAGAGGGATGGACAAAGACGCCAGATAACTATATCTCCTTTGTTGGTCTTCTTGCAGATTCACGTTATTGTGGTATTATACTAATCCTGGTATCTAGATATTCTCCATTCTTTATCATGGAATTTCATGCATTTTTCTTTTTCTTCTAAACATCTCGGATAAAATTACCCATACTCACTCAAAAATATGAACTGATATTTCTTATGAAACTTCAAAAGCAGCAAAGCTTGATTTATTGCTTGCCATCTTCATGTTAAGAAGCTCCATACTTCCTACTTATTTTGAGGGTCATGTTTCCATGAGCTGCATGGTTGGGGATGGTTAATTAACCAGAACCTCCTGCCTGAAAAGGAAAAAAAAAAGTATAAATTTAAATTTGGAAGCCATCACATGACTAATCCCTAGCGGTACGGTGGAGGGACAATAATTGCACTTGGTTAATTGGATCTGTGTCTCTAAGCGAAATTAATCGAGCTTGTGGGTGCTATAATAACATCAACATAAAGAACTTGATTTTAAATGGAAAGGGAGCTTCATAACACTTCTCGCCCCTTTCCATCTCAAAATATCGAGCTCTTAATTTCATAGACCAGGTACAAGCTTGAGAATTGTGATAAGATTATATTTGGAAACGTTATGTGGATTGCGCATTCATCTCTCGCATACTTTGTTTCTCATTCTTCATTTTGTGTAATGTTACAATTCTGGTACCAAGGAAATCAGAATGACCGACTAGGAGCCTAATTTTAGAGTCTAGAGGCATCGTACATTGATTGTGTACACTTAATTGGCACTTGACTTAGTCTTCGTAAGCATGAGGAGGTTGAGTAAAGTATAACCAAAGGGTTCAGTGCTCATGAGGCTGTGTAAGAGAATTGCATTTCATGCATTCTCCATTCAAAACTGTTGCTATTCGTGGTTGTGAAATGAGAGATTCTTGTTCTCATAAAGATGGCCGTATATTTTTTATTCCTCTTGGTTGCCGTTGTAATACCCTTTGTTTCATTTTTCTTTCTTTCCCTTGTTTGTTGCCTTGAAACGTTTCCTTCCTGGAGAAATCTACGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTGAAAATGTTGGTGGATGTCGCTTGGATCTAAATTGTTTGGCATGTTAATTTAATTATCAGAATTTTCTGGAAGGAATAATCTAGATTCTACGTCAAGTTCTTGTGGTCAGTGGAGAAGTTAACAAGCTTTTGTTATATGAATGCATTTCTTTATTATTTTATAACTTAATGCTTTTTGCTGCTAATGCTTACATTTTTATTCTATATTGAAATAATATGTAATGTAGGTGTATCATATGTGGAGAAGGAAGAGGTCCGCCTCCTACTTAGGCAGGTTAGTTTTTTCATGGTCGTATTGCTGTGGATAGTGTTGTATCTTGGCAAGAAGTTTCTATATATTAAGCATTGTTTTTCGTTATGAGGTATTTTATAGGTCAGATGTGCTTGATTCCTAAATGTAGAATCCTTCTTTTGGAGTAGACAAGTATAAATATAATGTAGAGACATTTCAAGTCCATTGAGTTAACAAAAGGGACATGGAAACTAAGATTATTGGTCTGCAGGTACTGTATAATTATGTCCATAATATGATAAGATTCAAAATGTTATCTCGTCATAAAATTATATGCAGGTATCGTGTAAATGTTCCACAAGTTTATGTATCTTTGCATAAAAACTAAATTCTAAATTTGTATTTGACAGGACCCGAAGTTTTGGACCTATAGACCATTGTCTGAATTGATGGTCCGTGCAGCTGCTGATGATGTACGCTTTCTGCTTTACATCTATCACAAGTTGATGGAGAAATTGAATCATCAATCGCTGTGGTACCTCGCAGTGCGTGGTGCTTTGTACTGCCGGTGTTTCTGCATCAATGATAATGGATATGCTAACTGGCCTCCTCTCCCTTCTGTTCCAGGTTTTCTTTAAGAACACACCTTACCATGTGCAGTCCATATAATGAGAATAATTCCAAATTTAACATTCTAATATGCTAAGTTGATTAAACTATCATTACAAACTTTTCAACATAATATGATTCTGTGGTGTTTCAATATTGTTGGCTTCTTTTATGTTATGATTTCACAATTGATATTGGGTTTAAAATTCAAAGTGGCTATTTAGCAATGTGAGAAATGTTGGCTCACCATCTTTCATTCAATTATCCCAATCTGCTGGAATGTGCAAAACATCAAATATATTAAAATTTATAATGTTGTATTATCTAATTATTATTGACACGTGGTATGTGGTTTCTGTCCTTAGGGTTCTTAGACTACGTTTCACTTCAAATCTTATTTTTTCATTTGATGCGTCTAAAACCTTTTGTTACATGGTCTCAATTGATCACCTTTATTGGTCTTACATGTTTTTTCAGATAACCTTGTAGTAGAGGACAAAGCTCCTGAAGAAGAAATTCTTTCAGTCTTAGACATTCCCCGTGGAATGATGGGTCGCGTAATTGGTAGGAGAGGAGCCTCAATTTTGGCAATAAAGGAATCTTGCAAGTAATTCACTATCGACAAATCCCTTTAGACTTCATTTATATCCTTTAGGCTAAAAAAATACCATAGCCGTTGTTTTTTCTCACCTTGGGTTCTTAAAATGCAGGGCGGAAATTCTCGTTGGCGGCACTAAGGGCCCACCGGACAAGGTAAGACTCTCTTTTCATTCACTTCAAAACAGACCACTATAAAGCCTAAAATAGAATGCTATTCTTTGAACTTTGCTGTGAATTTCCTGGATTCCTCTCTCCATTTGCTTCAAATCCCACTGCCTAGATTAGACCTCAACACCTCCTTTTGAGTTATTTGTTAAAACCCCCTGGAACAAACAAAGGGGACTCGCTCTGCTTGTCTCGAAGTCTCTGAGTATCAGCCAAAATCCTTTTGAATGTTGATGGACATTGAAAGTTGTTCTGTCAGTAAGATGAATGTAATTGTGGTGATGTAGGTTTTCGTCATTGGACCCGTGAGGCAGGTAAGGAAGGCAGAAGCTATGATACGAGGAAGCTTGCTTGAAATGTGATTGAATGAACATGAAAACCGTGTTGTGATGAACATGAACGCAAAATCCTCAATATTAAGAATACAGTTTCAGCTGCTAATACTAGTAGATCCCGTATGCAAGGGATTACTTTACATACTTCACAATCTTTTGTATAGGTACAGTAGCTTTGAGAAGCAAATTTTATGTGAAAAATATTATCAAATCAAAATATGAAAATTGAAATAAAACTTTGTTTTTACCTTCAAACTTAATAAATCTAAAATAAATTCG

mRNA sequence

AAATTGAAAGATTCGCTCAAATGGCGAACACTCCTTCTCGCCAGACGTATATTCCTCTGCCTTCGGACTCAGGTGAAAATCAGAACGATCCTGAAGCCAACAAAACCTTGGTTCCTATTCATATTGTTACTCATGCATCTCAACTCCCTAACGAATTTGTTGATCCATCACCTGAAAAGCCTCTGGTAGTTGGCTTTGATTGTGAAGGTGTCAGCCTGTGTCGCCATGGACGTCTTTGTATCATGCAGATTGCATTTCCAGATGCTATATATCTGGTTGATGCTGTTCAGGGAGGAGAGGAACTTGTGAAAGTCTGTAAGCCTGCCCTTGAGTCCACATATGTCACGAAAGTTATTCATGATTGTAAACGAGATAGTGAGGCATTGTACTTTCAGTTTGGTATTAAGTTGAACAACGTTATTGATACACAGATTGCATATTCACTTATAGAGGAGCAAGAGGGATGGACAAAGACGCCAGATAACTATATCTCCTTTGTTGGTCTTCTTGCAGATTCACGTTATTGTGGTGTATCATATGTGGAGAAGGAAGAGGTCCGCCTCCTACTTAGGCAGGACCCGAAGTTTTGGACCTATAGACCATTGTCTGAATTGATGGTCCGTGCAGCTGCTGATGATGTACGCTTTCTGCTTTACATCTATCACAAGTTGATGGAGAAATTGAATCATCAATCGCTGTGGTACCTCGCAGTGCGTGGTGCTTTGTACTGCCGGTGTTTCTGCATCAATGATAATGGATATGCTAACTGGCCTCCTCTCCCTTCTGTTCCAGATAACCTTGTAGTAGAGGACAAAGCTCCTGAAGAAGAAATTCTTTCAGTCTTAGACATTCCCCGTGGAATGATGGGTCGCGTAATTGGTAGGAGAGGAGCCTCAATTTTGGCAATAAAGGAATCTTGCAAGGCGGAAATTCTCGTTGGCGGCACTAAGGGCCCACCGGACAAGGTTTTCGTCATTGGACCCGTGAGGCAGGTAAGGAAGGCAGAAGCTATGATACGAGGAAGCTTGCTTGAAATGTGATTGAATGAACATGAAAACCGTGTTGTGATGAACATGAACGCAAAATCCTCAATATTAAGAATACAGTTTCAGCTGCTAATACTAGTAGATCCCGTATGCAAGGGATTACTTTACATACTTCACAATCTTTTGTATAGGTACAGTAGCTTTGAGAAGCAAATTTTATGTGAAAAATATTATCAAATCAAAATATGAAAATTGAAATAAAACTTTGTTTTTACCTTCAAACTTAATAAATCTAAAATAAATTCG

Coding sequence (CDS)

ATGGCGAACACTCCTTCTCGCCAGACGTATATTCCTCTGCCTTCGGACTCAGGTGAAAATCAGAACGATCCTGAAGCCAACAAAACCTTGGTTCCTATTCATATTGTTACTCATGCATCTCAACTCCCTAACGAATTTGTTGATCCATCACCTGAAAAGCCTCTGGTAGTTGGCTTTGATTGTGAAGGTGTCAGCCTGTGTCGCCATGGACGTCTTTGTATCATGCAGATTGCATTTCCAGATGCTATATATCTGGTTGATGCTGTTCAGGGAGGAGAGGAACTTGTGAAAGTCTGTAAGCCTGCCCTTGAGTCCACATATGTCACGAAAGTTATTCATGATTGTAAACGAGATAGTGAGGCATTGTACTTTCAGTTTGGTATTAAGTTGAACAACGTTATTGATACACAGATTGCATATTCACTTATAGAGGAGCAAGAGGGATGGACAAAGACGCCAGATAACTATATCTCCTTTGTTGGTCTTCTTGCAGATTCACGTTATTGTGGTGTATCATATGTGGAGAAGGAAGAGGTCCGCCTCCTACTTAGGCAGGACCCGAAGTTTTGGACCTATAGACCATTGTCTGAATTGATGGTCCGTGCAGCTGCTGATGATGTACGCTTTCTGCTTTACATCTATCACAAGTTGATGGAGAAATTGAATCATCAATCGCTGTGGTACCTCGCAGTGCGTGGTGCTTTGTACTGCCGGTGTTTCTGCATCAATGATAATGGATATGCTAACTGGCCTCCTCTCCCTTCTGTTCCAGATAACCTTGTAGTAGAGGACAAAGCTCCTGAAGAAGAAATTCTTTCAGTCTTAGACATTCCCCGTGGAATGATGGGTCGCGTAATTGGTAGGAGAGGAGCCTCAATTTTGGCAATAAAGGAATCTTGCAAGGCGGAAATTCTCGTTGGCGGCACTAAGGGCCCACCGGACAAGGTTTTCGTCATTGGACCCGTGAGGCAGGTAAGGAAGGCAGAAGCTATGATACGAGGAAGCTTGCTTGAAATGTGA

Protein sequence

MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Homology
BLAST of CmoCh05G001160 vs. ExPASy Swiss-Prot
Match: Q8NHP7 (piRNA biogenesis protein EXD1 OS=Homo sapiens OX=9606 GN=EXD1 PE=1 SV=4)

HSP 1 Score: 89.0 bits (219), Expect = 1.1e-16
Identity = 53/172 (30.81%), Postives = 89/172 (51.74%), Query Frame = 0

Query: 52  EKPLVVGFDCEGVSLCRHGRLCIMQIAFPDAIYLVDA-VQGGEELVKVCKPALESTYVTK 111
           +K  V+    EG ++CRHG+LC +Q+A    +YL D  + G        +  LE   + K
Sbjct: 96  KKQNVLSVAAEGANVCRHGKLCWLQVATNCRVYLFDIFLLGSRAFHNGLQMILEDKRILK 155

Query: 112 VIHDCKRDSEALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCG 171
           VIHDC+  S+ L  Q+GI LNNV DTQ+A  L    E     P+   +    L       
Sbjct: 156 VIHDCRWLSDCLSHQYGILLNNVFDTQVADVLQFSMETGGYLPNCITTLQESLIKHLQVA 215

Query: 172 VSYVE-KEEVRLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKL 222
             Y+   E+ + L++++P+ W  RP+S  +++  A +  +LL +   L++++
Sbjct: 216 PKYLSFLEKRQKLIQENPEVWFIRPVSPSLLKILALEATYLLPLRLALLDEM 267

BLAST of CmoCh05G001160 vs. ExPASy Swiss-Prot
Match: Q8CDF7 (piRNA biogenesis protein EXD1 OS=Mus musculus OX=10090 GN=Exd1 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 5.7e-16
Identity = 55/173 (31.79%), Postives = 89/173 (51.45%), Query Frame = 0

Query: 52  EKPLVVGFDCEGVSLCRHGRLCIMQIAFPDAIYLVDA-VQGGEELVKVCKPALESTYVTK 111
           +K  V+    EG ++CRHG+LC +Q+A    +YL D  + G        +  LE   + K
Sbjct: 153 KKQSVLSVAAEGANVCRHGKLCWLQVATNSRVYLFDIFLLGSRAFNNGLQMILEDKRILK 212

Query: 112 VIHDCKRDSEALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFV--GLLADSRY 171
           VIHDC+  S+ L  Q+GI LNNV DTQ+A  L    E     P N IS +   L+   + 
Sbjct: 213 VIHDCRWLSDCLSHQYGIMLNNVFDTQVADVLQFSMETGGFLP-NCISTLQESLIRHLKV 272

Query: 172 CGVSYVEKEEVRLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKL 222
                   EE +  ++++P+ W  RPL   +++  A +  +LL +   L++++
Sbjct: 273 APRYLFFLEERQKRIQENPEIWLTRPLPPSLLKILALETTYLLPLRLVLLDEV 324

BLAST of CmoCh05G001160 vs. ExPASy Swiss-Prot
Match: Q6NRD5 (piRNA biogenesis protein EXD1 OS=Xenopus laevis OX=8355 GN=exd1 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.2e-11
Identity = 47/173 (27.17%), Postives = 86/173 (49.71%), Query Frame = 0

Query: 56  VVGFDCEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVK-VCKPALESTYVTKVIHD 115
           V+     G ++CRHG+L  +Q A    +YL D +  G ++ K   +  LE   + KVIHD
Sbjct: 122 VISIGAVGQNICRHGKLSWLQFATRSRVYLFDVLVLGSKVFKNGLQMVLEDKGILKVIHD 181

Query: 116 CKRDSEALYFQFGIKLNNVIDTQI--AYSLIEEQEGW----TKTPDNYISFVGLLADSRY 175
           C+   + L  Q+GI LNNV DTQ+   Y    E  G+    T+T +  +     +  S+ 
Sbjct: 182 CRWLGDILSHQYGIILNNVFDTQVGDVYLFSMETGGFLPHGTRTLEECLIHHLSMLPSK- 241

Query: 176 CGVSYVEKEEVRLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKL 222
             VS++   +   L ++    W  RP+   +++  + +V +L+ +   +++ +
Sbjct: 242 --VSFLAHRQT--LTKEYHDIWFDRPMDPTLLKLLSLEVTYLMPLRSAMLDAM 289

BLAST of CmoCh05G001160 vs. ExPASy Swiss-Prot
Match: Q0P3U3 (piRNA biogenesis protein EXD1 OS=Danio rerio OX=7955 GN=exd1 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.0e-09
Identity = 46/170 (27.06%), Postives = 77/170 (45.29%), Query Frame = 0

Query: 56  VVGFDCEGVSLCRHGRLCIMQIAFPDAIYLVD-AVQGGEELVKVCKPALESTYVTKVIHD 115
           V+G   +        RLC +Q+A    +YL D  + GG          LE+T++ KV+HD
Sbjct: 136 VIGIGADVYGQSGQERLCWLQVATKKVVYLFDILLLGGPAFKNGLSMILENTHILKVLHD 195

Query: 116 CKRDSEALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYV 175
           C+  +  L  +F ++L NV DTQ+A  L+   E     PD   S   LL    +  ++  
Sbjct: 196 CRCITRCLRTEFRVQLTNVFDTQVAELLLFFNESGGFLPDRPASLPELL--QLHLRLTTA 255

Query: 176 EKEEV---RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKL 222
           E + +   +   R+  + W  RP    ++      V+ LL +   L++ L
Sbjct: 256 EIQPLCSKQQQSRECVQLWYVRPCPPDLLSLMCSSVQHLLSLRLLLLDAL 303

BLAST of CmoCh05G001160 vs. ExPASy Swiss-Prot
Match: P56960 (Exosome component 10 OS=Mus musculus OX=10090 GN=Exosc10 PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 1.3e-07
Identity = 45/159 (28.30%), Postives = 71/159 (44.65%), Query Frame = 0

Query: 70  GRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIK 129
           G  C+MQI+     ++VD ++   ++  +   +L    + KV H    D E L   FG+ 
Sbjct: 324 GLTCLMQISTRTEDFIVDTLELRSDMY-ILNESLTDPAIVKVFHGADSDIEWLQKDFGLY 383

Query: 130 LNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKF 189
           + N+ DT  A  L+        + D+ +          YCGV   ++ ++          
Sbjct: 384 VVNMFDTHQAARLLNLAR---HSLDHLLRL--------YCGVESNKQYQL--------AD 443

Query: 190 WTYRPLSELMVRAAADDVRFLLYIYHK----LMEKLNHQ 225
           W  RPL E M+  A DD  +LLYIY +    L E+ NHQ
Sbjct: 444 WRIRPLPEEMLSYARDDTHYLLYIYDRMRLELWERGNHQ 462

BLAST of CmoCh05G001160 vs. ExPASy TrEMBL
Match: A0A6J1HD17 (piRNA biogenesis protein EXD1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462393 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 5.2e-198
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD 60
           MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD
Sbjct: 1   MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD 60

Query: 61  CEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE 120
           CEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE
Sbjct: 61  CEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE 120

Query: 121 ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR 180
           ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR
Sbjct: 121 ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR 180

Query: 181 LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF 240
           LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF
Sbjct: 181 LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF 240

Query: 241 CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 300
           CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC
Sbjct: 241 CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 300

Query: 301 KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 339

BLAST of CmoCh05G001160 vs. ExPASy TrEMBL
Match: A0A6J1K589 (piRNA biogenesis protein EXD1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492408 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 8.6e-193
Identity = 331/339 (97.64%), Postives = 334/339 (98.53%), Query Frame = 0

Query: 1   MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD 60
           MANTPS QTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD
Sbjct: 1   MANTPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD 60

Query: 61  CEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE 120
           CEGVSLC HG LCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE
Sbjct: 61  CEGVSLCCHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE 120

Query: 121 ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR 180
           ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR
Sbjct: 121 ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR 180

Query: 181 LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF 240
           LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF
Sbjct: 181 LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF 240

Query: 241 CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 300
           CI+DNGYA+WPPLPSVPDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC
Sbjct: 241 CISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 300

Query: 301 KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           KAEILVGG KGPPDKVFVIGPVRQVRKAEAMIRGSLLE+
Sbjct: 301 KAEILVGGAKGPPDKVFVIGPVRQVRKAEAMIRGSLLEI 339

BLAST of CmoCh05G001160 vs. ExPASy TrEMBL
Match: A0A6J1HB37 (piRNA biogenesis protein EXD1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462393 PE=4 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 4.9e-188
Identity = 322/323 (99.69%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 17  SGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQ 76
           +GENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQ
Sbjct: 11  NGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQ 70

Query: 77  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 136
           IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT
Sbjct: 71  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 130

Query: 137 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 196
           QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS
Sbjct: 131 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 190

Query: 197 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSV 256
           ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSV
Sbjct: 191 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSV 250

Query: 257 PDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 316
           PDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV
Sbjct: 251 PDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 310

Query: 317 FVIGPVRQVRKAEAMIRGSLLEM 340
           FVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 311 FVIGPVRQVRKAEAMIRGSLLEM 333

BLAST of CmoCh05G001160 vs. ExPASy TrEMBL
Match: A0A6J1HET0 (exosome complex exonuclease rrp6-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462393 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.4e-187
Identity = 321/323 (99.38%), Postives = 322/323 (99.69%), Query Frame = 0

Query: 17  SGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQ 76
           + ENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQ
Sbjct: 20  ASENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQ 79

Query: 77  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 136
           IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT
Sbjct: 80  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 139

Query: 137 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 196
           QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS
Sbjct: 140 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 199

Query: 197 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSV 256
           ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSV
Sbjct: 200 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSV 259

Query: 257 PDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 316
           PDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV
Sbjct: 260 PDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 319

Query: 317 FVIGPVRQVRKAEAMIRGSLLEM 340
           FVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 320 FVIGPVRQVRKAEAMIRGSLLEM 342

BLAST of CmoCh05G001160 vs. ExPASy TrEMBL
Match: A0A6J1KE47 (piRNA biogenesis protein EXD1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492408 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.1e-183
Identity = 315/323 (97.52%), Postives = 319/323 (98.76%), Query Frame = 0

Query: 17  SGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQ 76
           +GENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLC HG LCIMQ
Sbjct: 11  NGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCCHGSLCIMQ 70

Query: 77  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 136
           IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT
Sbjct: 71  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 130

Query: 137 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 196
           QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS
Sbjct: 131 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 190

Query: 197 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSV 256
           ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCI+DNGYA+WPPLPSV
Sbjct: 191 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSV 250

Query: 257 PDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 316
           PDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGG KGPPDKV
Sbjct: 251 PDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGAKGPPDKV 310

Query: 317 FVIGPVRQVRKAEAMIRGSLLEM 340
           FVIGPVRQVRKAEAMIRGSLLE+
Sbjct: 311 FVIGPVRQVRKAEAMIRGSLLEI 333

BLAST of CmoCh05G001160 vs. NCBI nr
Match: XP_022961700.1 (piRNA biogenesis protein EXD1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 699.9 bits (1805), Expect = 1.1e-197
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD 60
           MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD
Sbjct: 1   MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD 60

Query: 61  CEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE 120
           CEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE
Sbjct: 61  CEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE 120

Query: 121 ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR 180
           ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR
Sbjct: 121 ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR 180

Query: 181 LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF 240
           LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF
Sbjct: 181 LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF 240

Query: 241 CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 300
           CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC
Sbjct: 241 CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 300

Query: 301 KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 339

BLAST of CmoCh05G001160 vs. NCBI nr
Match: KAG6598308.1 (piRNA biogenesis protein EXD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 686.0 bits (1769), Expect = 1.6e-193
Identity = 335/340 (98.53%), Postives = 336/340 (98.82%), Query Frame = 0

Query: 1   MANT-PSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANT PS QTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLCRHG LCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIY KLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYRKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCINDNGYA+WPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCINDNGYADWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340

BLAST of CmoCh05G001160 vs. NCBI nr
Match: KAG7029279.1 (piRNA biogenesis protein EXD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 685.6 bits (1768), Expect = 2.1e-193
Identity = 334/340 (98.24%), Postives = 336/340 (98.82%), Query Frame = 0

Query: 1   MANT-PSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANT PS QTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLCRHG LCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEE+
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEI 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIY KLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYRKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCINDNGYA+WPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCINDNGYADWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340

BLAST of CmoCh05G001160 vs. NCBI nr
Match: XP_023545692.1 (piRNA biogenesis protein EXD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 684.5 bits (1765), Expect = 4.7e-193
Identity = 334/340 (98.24%), Postives = 336/340 (98.82%), Query Frame = 0

Query: 1   MANT-PSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANT PS QTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLCRHG LCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCI+DNGYA+WPPLPSVPDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340

BLAST of CmoCh05G001160 vs. NCBI nr
Match: XP_022997512.1 (piRNA biogenesis protein EXD1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 682.6 bits (1760), Expect = 1.8e-192
Identity = 331/339 (97.64%), Postives = 334/339 (98.53%), Query Frame = 0

Query: 1   MANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD 60
           MANTPS QTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD
Sbjct: 1   MANTPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFD 60

Query: 61  CEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE 120
           CEGVSLC HG LCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE
Sbjct: 61  CEGVSLCCHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSE 120

Query: 121 ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR 180
           ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR
Sbjct: 121 ALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVR 180

Query: 181 LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF 240
           LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF
Sbjct: 181 LLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF 240

Query: 241 CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 300
           CI+DNGYA+WPPLPSVPDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC
Sbjct: 241 CISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 300

Query: 301 KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           KAEILVGG KGPPDKVFVIGPVRQVRKAEAMIRGSLLE+
Sbjct: 301 KAEILVGGAKGPPDKVFVIGPVRQVRKAEAMIRGSLLEI 339

BLAST of CmoCh05G001160 vs. TAIR 10
Match: AT2G25910.1 (3'-5' exonuclease domain-containing protein / K homology domain-containing protein / KH domain-containing protein )

HSP 1 Score: 515.0 bits (1325), Expect = 4.6e-146
Identity = 237/338 (70.12%), Postives = 291/338 (86.09%), Query Frame = 0

Query: 2   ANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDC 61
           +++P++  ++P+P + G      EAN+  VPI+IVT   QLP +F++PSPEK LV+GFDC
Sbjct: 3   SSSPTQLAHVPIPPEPGGRSPTQEANEPPVPIYIVTDPFQLPADFLNPSPEKKLVIGFDC 62

Query: 62  EGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEA 121
           EGV LCRHG+LCIMQIAF +AIYLVD ++GGE ++K CKPALES Y+TKVIHDCKRDSEA
Sbjct: 63  EGVDLCRHGKLCIMQIAFSNAIYLVDVIEGGEVIMKACKPALESNYITKVIHDCKRDSEA 122

Query: 122 LYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRL 181
           LYFQFGI+L+NV+DTQIAYSLIEEQEG  +  D+YISFV LLAD RYCG+SY EKEEVR+
Sbjct: 123 LYFQFGIRLHNVVDTQIAYSLIEEQEGRRRPLDDYISFVSLLADPRYCGISYEEKEEVRV 182

Query: 182 LLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF- 241
           L+RQDPKFWTYRP++ELM+RAAADDVRFLLY+YHK+M KLN +SLW+LAVRGALYCRC  
Sbjct: 183 LMRQDPKFWTYRPMTELMIRAAADDVRFLLYLYHKMMGKLNQRSLWHLAVRGALYCRCLC 242

Query: 242 CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 301
           C+ND  +A+WP +P +PDNL  ED+  EEEILSVLD+P G MGRVIGR+GASILAIKE+C
Sbjct: 243 CMNDADFADWPTVPPIPDNLKSEDQCLEEEILSVLDVPPGKMGRVIGRKGASILAIKEAC 302

Query: 302 KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLE 339
            AEIL+GG KGPPDK+FVIGPVR+VRKAEA++RG +++
Sbjct: 303 NAEILIGGAKGPPDKIFVIGPVREVRKAEAILRGRMID 340

BLAST of CmoCh05G001160 vs. TAIR 10
Match: AT2G25910.2 (3'-5' exonuclease domain-containing protein / K homology domain-containing protein / KH domain-containing protein )

HSP 1 Score: 510.4 bits (1313), Expect = 1.1e-144
Identity = 237/339 (69.91%), Postives = 291/339 (85.84%), Query Frame = 0

Query: 2   ANTPSRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDC 61
           +++P++  ++P+P + G      EAN+  VPI+IVT   QLP +F++PSPEK LV+GFDC
Sbjct: 3   SSSPTQLAHVPIPPEPGGRSPTQEANEPPVPIYIVTDPFQLPADFLNPSPEKKLVIGFDC 62

Query: 62  EGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEA 121
           EGV LCRHG+LCIMQIAF +AIYLVD ++GGE ++K CKPALES Y+TKVIHDCKRDSEA
Sbjct: 63  EGVDLCRHGKLCIMQIAFSNAIYLVDVIEGGEVIMKACKPALESNYITKVIHDCKRDSEA 122

Query: 122 LYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRL 181
           LYFQFGI+L+NV+DTQIAYSLIEEQEG  +  D+YISFV LLAD RYCG+SY EKEEVR+
Sbjct: 123 LYFQFGIRLHNVVDTQIAYSLIEEQEGRRRPLDDYISFVSLLADPRYCGISYEEKEEVRV 182

Query: 182 LLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCF- 241
           L+RQDPKFWTYRP++ELM+RAAADDVRFLLY+YHK+M KLN +SLW+LAVRGALYCRC  
Sbjct: 183 LMRQDPKFWTYRPMTELMIRAAADDVRFLLYLYHKMMGKLNQRSLWHLAVRGALYCRCLC 242

Query: 242 CINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESC 301
           C+ND  +A+WP +P +PDNL  ED+  EEEILSVLD+P G MGRVIGR+GASILAIKE+C
Sbjct: 243 CMNDADFADWPTVPPIPDNLKSEDQCLEEEILSVLDVPPGKMGRVIGRKGASILAIKEAC 302

Query: 302 -KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLE 339
             AEIL+GG KGPPDK+FVIGPVR+VRKAEA++RG +++
Sbjct: 303 NSAEILIGGAKGPPDKIFVIGPVREVRKAEAILRGRMID 341

BLAST of CmoCh05G001160 vs. TAIR 10
Match: AT1G54440.1 (Polynucleotidyl transferase, ribonuclease H fold protein with HRDC domain )

HSP 1 Score: 58.2 bits (139), Expect = 1.5e-08
Identity = 45/153 (29.41%), Postives = 67/153 (43.79%), Query Frame = 0

Query: 70  GRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIK 129
           G  C+MQI+     Y+VD  +  + +    +   +     KVIH   RD   L   FGI 
Sbjct: 151 GLTCLMQISTRTEDYIVDIFKLWDHIGPYLRELFKDPKKKKVIHGADRDIIWLQRDFGIY 210

Query: 130 LNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKF 189
           + N+ DT  A  ++       K   N + F+       YCGV+   KE            
Sbjct: 211 VCNLFDTGQASRVL-------KLERNSLEFL----LKHYCGVA-ANKE-------YQKAD 270

Query: 190 WTYRPLSELMVRAAADDVRFLLYIYHKLMEKLN 223
           W  RPL ++M R A +D  +LLYIY  +  +L+
Sbjct: 271 WRIRPLPDVMKRYAREDTHYLLYIYDVMRMELH 284

BLAST of CmoCh05G001160 vs. TAIR 10
Match: AT1G54440.2 (Polynucleotidyl transferase, ribonuclease H fold protein with HRDC domain )

HSP 1 Score: 58.2 bits (139), Expect = 1.5e-08
Identity = 45/153 (29.41%), Postives = 67/153 (43.79%), Query Frame = 0

Query: 70  GRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIK 129
           G  C+MQI+     Y+VD  +  + +    +   +     KVIH   RD   L   FGI 
Sbjct: 151 GLTCLMQISTRTEDYIVDIFKLWDHIGPYLRELFKDPKKKKVIHGADRDIIWLQRDFGIY 210

Query: 130 LNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKF 189
           + N+ DT  A  ++       K   N + F+       YCGV+   KE            
Sbjct: 211 VCNLFDTGQASRVL-------KLERNSLEFL----LKHYCGVA-ANKE-------YQKAD 270

Query: 190 WTYRPLSELMVRAAADDVRFLLYIYHKLMEKLN 223
           W  RPL ++M R A +D  +LLYIY  +  +L+
Sbjct: 271 WRIRPLPDVMKRYAREDTHYLLYIYDVMRMELH 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8NHP71.1e-1630.81piRNA biogenesis protein EXD1 OS=Homo sapiens OX=9606 GN=EXD1 PE=1 SV=4[more]
Q8CDF75.7e-1631.79piRNA biogenesis protein EXD1 OS=Mus musculus OX=10090 GN=Exd1 PE=1 SV=1[more]
Q6NRD57.2e-1127.17piRNA biogenesis protein EXD1 OS=Xenopus laevis OX=8355 GN=exd1 PE=2 SV=1[more]
Q0P3U33.0e-0927.06piRNA biogenesis protein EXD1 OS=Danio rerio OX=7955 GN=exd1 PE=2 SV=1[more]
P569601.3e-0728.30Exosome component 10 OS=Mus musculus OX=10090 GN=Exosc10 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1HD175.2e-198100.00piRNA biogenesis protein EXD1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1K5898.6e-19397.64piRNA biogenesis protein EXD1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HB374.9e-18899.69piRNA biogenesis protein EXD1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1HET02.4e-18799.38exosome complex exonuclease rrp6-like isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1KE472.1e-18397.52piRNA biogenesis protein EXD1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_022961700.11.1e-197100.00piRNA biogenesis protein EXD1-like isoform X2 [Cucurbita moschata][more]
KAG6598308.11.6e-19398.53piRNA biogenesis protein EXD1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7029279.12.1e-19398.24piRNA biogenesis protein EXD1, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_023545692.14.7e-19398.24piRNA biogenesis protein EXD1-like [Cucurbita pepo subsp. pepo][more]
XP_022997512.11.8e-19297.64piRNA biogenesis protein EXD1-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G25910.14.6e-14670.123'-5' exonuclease domain-containing protein / K homology domain-containing prote... [more]
AT2G25910.21.1e-14469.913'-5' exonuclease domain-containing protein / K homology domain-containing prote... [more]
AT1G54440.11.5e-0829.41Polynucleotidyl transferase, ribonuclease H fold protein with HRDC domain [more]
AT1G54440.21.5e-0829.41Polynucleotidyl transferase, ribonuclease H fold protein with HRDC domain [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004087K Homology domainSMARTSM00322kh_6coord: 269..337
e-value: 9.6E-8
score: 41.7
IPR0025623'-5' exonuclease domainSMARTSM0047435exoneu6coord: 33..222
e-value: 6.2E-25
score: 98.8
IPR0025623'-5' exonuclease domainPFAMPF01612DNA_pol_A_exo1coord: 45..221
e-value: 1.6E-23
score: 83.4
IPR036612K Homology domain, type 1 superfamilyGENE3D3.30.1370.10K Homology domain, type 1coord: 259..336
e-value: 2.8E-10
score: 41.7
IPR036612K Homology domain, type 1 superfamilySUPERFAMILY54791Eukaryotic type KH-domain (KH-domain type I)coord: 269..336
IPR004088K Homology domain, type 1PFAMPF00013KH_1coord: 275..333
e-value: 1.9E-9
score: 37.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 4..235
e-value: 1.5E-41
score: 144.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR46814EGALITARIAN, ISOFORM Bcoord: 2..336
NoneNo IPR availablePANTHERPTHR46814:SF4SUBFAMILY NOT NAMEDcoord: 2..336
NoneNo IPR availablePROSITEPS50084KH_TYPE_1coord: 270..332
score: 11.594319
NoneNo IPR availableCDDcd06148Egl_like_exocoord: 46..241
e-value: 8.61114E-79
score: 237.568
NoneNo IPR availableCDDcd00105KH-Icoord: 275..332
e-value: 3.815E-9
score: 50.2511
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 27..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G001160.1CmoCh05G001160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006139 nucleobase-containing compound metabolic process
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding