CmoCh04G028600 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh04G028600
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	metalloendoproteinase 1-like
Location	Cmo_Chr04: 20373775 .. 20374686 (+)
RNA-Seq Expression	CmoCh04G028600
Synteny	CmoCh04G028600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTGACATCATGTCTCCAACTGATGTTCATACTTGTCGCCGCCATAGCACCCCACGCCATCATGGCGCGCCATCTTCACACCCACAAGTTGTCCTCGTCTATATTTCCCCAACATCTCGTAGGAAGTGGGAAGGGCCACAACATAGAAGGAATCCACAACGTCAGAGCCTTCCTCCAACGTTATGGTTACTTGAGCACAAATGTGGAGACCAAAGGCAACGTCTTTGATGACAACTTGGAGTTTGCCATTAAATCATACCAAAAATTCTTCAACCTCAACGTGAGTGGCATTTTGGATGCGGAGACTTTGGAGCTAATGTCGCAGCCTCGATGTGGAGTTCCCGATGTGTTCATCAACGAGACGAGCGGAGATAGGAACGGGGACGGGGATCACCCCATAGGACTTCATTACGCATTTATCAATGATTTGAAACCGAAATGGGGGGCGTCGAAATACAATCTGAAATACGCATTTGTTGGGAGTTTTCCAAAACAGTACAAGGCTACAGTGGTTGGAGCCATGCACAGTTGGAGTTCAAAAAGTGGATTTAAATTCACAAAAGGTAGTGCGAATAAGGCAGATGTTAAAATAAGCTTTGAAGTAGGAGACCATGGAGATGGGCATCCTTTCAAGGAGGGAAGTGGAGAATTGGGGCATGCTTTTGCGCCTGAAGATGGGAGACTTCACTTCAATGCGCGGGAGGCTTGGGCGGATGGAGCTTATCCTGATAAGTTCGATGTGGGGAGTGTGGCATTGCATGAGCTTGGACATATTCTTGGGCTGGGCCACAGCAGCTTTAGAAACGCTGTCATGTGGCCATCTATTGGTCCTAATTTTGTGATGGGCCTAAACGACGATGATGTTAATGGACTCTTGGCTTTGTATAATGGATTTAGGGAAAAATAG

mRNA sequence

ATGGATTTGACATCATGTCTCCAACTGATGTTCATACTTGTCGCCGCCATAGCACCCCACGCCATCATGGCGCGCCATCTTCACACCCACAAGTTGTCCTCGTCTATATTTCCCCAACATCTCGTAGGAAGTGGGAAGGGCCACAACATAGAAGGAATCCACAACGTCAGAGCCTTCCTCCAACGTTATGGTTACTTGAGCACAAATGTGGAGACCAAAGGCAACGTCTTTGATGACAACTTGGAGTTTGCCATTAAATCATACCAAAAATTCTTCAACCTCAACGTGAGTGGCATTTTGGATGCGGAGACTTTGGAGCTAATGTCGCAGCCTCGATGTGGAGTTCCCGATGTGTTCATCAACGAGACGAGCGGAGATAGGAACGGGGACGGGGATCACCCCATAGGACTTCATTACGCATTTATCAATGATTTGAAACCGAAATGGGGGGCGTCGAAATACAATCTGAAATACGCATTTGTTGGGAGTTTTCCAAAACAGTACAAGGCTACAGTGGTTGGAGCCATGCACAGTTGGAGTTCAAAAAGTGGATTTAAATTCACAAAAGGTAGTGCGAATAAGGCAGATGTTAAAATAAGCTTTGAAGTAGGAGACCATGGAGATGGGCATCCTTTCAAGGAGGGAAGTGGAGAATTGGGGCATGCTTTTGCGCCTGAAGATGGGAGACTTCACTTCAATGCGCGGGAGGCTTGGGCGGATGGAGCTTATCCTGATAAGTTCGATGTGGGGAGTGTGGCATTGCATGAGCTTGGACATATTCTTGGGCTGGGCCACAGCAGCTTTAGAAACGCTGTCATGTGGCCATCTATTGGTCCTAATTTTGTGATGGGCCTAAACGACGATGATGTTAATGGACTCTTGGCTTTGTATAATGGATTTAGGGAAAAATAG

Coding sequence (CDS)

ATGGATTTGACATCATGTCTCCAACTGATGTTCATACTTGTCGCCGCCATAGCACCCCACGCCATCATGGCGCGCCATCTTCACACCCACAAGTTGTCCTCGTCTATATTTCCCCAACATCTCGTAGGAAGTGGGAAGGGCCACAACATAGAAGGAATCCACAACGTCAGAGCCTTCCTCCAACGTTATGGTTACTTGAGCACAAATGTGGAGACCAAAGGCAACGTCTTTGATGACAACTTGGAGTTTGCCATTAAATCATACCAAAAATTCTTCAACCTCAACGTGAGTGGCATTTTGGATGCGGAGACTTTGGAGCTAATGTCGCAGCCTCGATGTGGAGTTCCCGATGTGTTCATCAACGAGACGAGCGGAGATAGGAACGGGGACGGGGATCACCCCATAGGACTTCATTACGCATTTATCAATGATTTGAAACCGAAATGGGGGGCGTCGAAATACAATCTGAAATACGCATTTGTTGGGAGTTTTCCAAAACAGTACAAGGCTACAGTGGTTGGAGCCATGCACAGTTGGAGTTCAAAAAGTGGATTTAAATTCACAAAAGGTAGTGCGAATAAGGCAGATGTTAAAATAAGCTTTGAAGTAGGAGACCATGGAGATGGGCATCCTTTCAAGGAGGGAAGTGGAGAATTGGGGCATGCTTTTGCGCCTGAAGATGGGAGACTTCACTTCAATGCGCGGGAGGCTTGGGCGGATGGAGCTTATCCTGATAAGTTCGATGTGGGGAGTGTGGCATTGCATGAGCTTGGACATATTCTTGGGCTGGGCCACAGCAGCTTTAGAAACGCTGTCATGTGGCCATCTATTGGTCCTAATTTTGTGATGGGCCTAAACGACGATGATGTTAATGGACTCTTGGCTTTGTATAATGGATTTAGGGAAAAATAG

Protein sequence

MDLTSCLQLMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFREK

Homology

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BLAST of CmoCh04G028600 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 176.0 bits (445), Expect = 6.4e-43
Identity = 105/261 (40.23%), Postives = 139/261 (53.26%), Query Frame = 0


Query: 47  GHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLE 106
           G N +G+ NV+ +    GY+        N FDD L  AIK+YQK +NLNV+G  D  TL+
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLK 107

Query: 107 LMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGS--F 166
            +  PRCGVPD+ IN       G     +   Y F  D+ P+W A    L YAF      
Sbjct: 108 QIMTPRCGVPDIIINTNKTTSFG-----MISDYTFFKDM-PRWQAGTTQLTYAFSPEPRL 167

Query: 167 PKQYKATVVGAMHSWSSKSGFKFTK-GSANKADVKISFEVGDHGDGHPFKEGSGELGHAF 226
              +K+ +  A   W+      F +  S   A++KI F   +HGD +PF    G LGHAF
Sbjct: 168 DDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAF 227

Query: 227 APEDGRLHFNAREAWADGAYPDK------FDVGSVALHELGHILGLGHSSFRNAVMWPSI 286
           AP DGR HF+A E W       K      FD+ SVA+HE+GH+LGLGHSS   A+M+PSI
Sbjct: 228 APTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSI 287

Query: 287 GPNF-VMGLNDDDVNGLLALY 298
            P    + L  DD++G+  LY
Sbjct: 288 PPRTRKVNLAQDDIDGIRKLY 301

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BLAST of CmoCh04G028600 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.3e-40
Identity = 119/315 (37.78%), Postives = 157/315 (49.84%), Query Frame = 0


Query: 9   LMFILVAAIAPHAIMARHLHTHKLSSSIFPQ-------------HLVGSGKGHNIEGIHN 68
           L F+L+    P+   AR+    + S++   Q              LV    G ++ G+  
Sbjct: 13  LCFVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSE 72

Query: 69  VRAFLQRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGV 128
           ++ +L R+GY++   E   +VFD  LE AI  YQ+   L ++G LD  T+ LMS PRCGV
Sbjct: 73  LKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGV 132

Query: 129 PDVFINETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQY------K 188
            D     T    N D  H    HY + N  KPKW  ++  L YA   +    Y      K
Sbjct: 133 SD-----THMTINNDFLHTTA-HYTYFNG-KPKW--NRDTLTYAISKTHKLDYLTSEDVK 192

Query: 189 ATVVGAMHSWSSKSGFKFTK-GSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDG 248
                A   WSS     F +      AD+KI F  GDHGDG PF    G L HAFAPE+G
Sbjct: 193 TVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENG 252

Query: 249 RLHFNAREAW-----ADGAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNF-V 298
           RLH +A E W       G+     D+ SVA HE+GH+LGLGHSS  +AVM+PS+ P    
Sbjct: 253 RLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKK 312

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BLAST of CmoCh04G028600 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-39
Identity = 104/281 (37.01%), Postives = 150/281 (53.38%), Query Frame = 0


Query: 40  HLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNV---FDDNLEFAIKSYQKFFNLNV 99
           +  G   G N++G++ ++ + QR+GY+       GN    FDD L+ A++ YQ  FNLNV
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETF--SGNFTDDFDDILKAAVELYQTNFNLNV 105

Query: 100 SGILDAETLELMSQPRCGVPDVFINETS---GDR------NGDGDHPIGLHYAFINDLKP 159
           +G LDA T++ +  PRCG PDV +N TS   G R      N    H   +    +   +P
Sbjct: 106 TGELDALTIQHIVIPRCGNPDV-VNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEP 165

Query: 160 KWGASKYNLKYAFVGSFP--KQYKATVVGAMHSWSSKSGFKFT-KGSANKADVKISFEVG 219
           +W  ++ +L YAF    P  ++ K+    A   WS  +   FT   S + +D+ I F  G
Sbjct: 166 RWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 225

Query: 220 DHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKF-------DVGSVALHEL 279
           DHGDG PF    G L HAF+P  G+ H +A E W      D F       D+ SVA+HE+
Sbjct: 226 DHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEI 285

Query: 280 GHILGLGHSSFRNAVMWPSIGP-NFVMGLNDDDVNGLLALY 298
           GH+LGLGHSS   ++M+P+I      + L +DDV G+  LY
Sbjct: 286 GHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

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BLAST of CmoCh04G028600 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.9e-39
Identity = 101/272 (37.13%), Postives = 147/272 (54.04%), Query Frame = 0


Query: 41  LVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGIL 100
           L G   G NI G+  ++ + +R+GY++T      + FDD L+ AI +YQK FNL V+G L
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-FDDVLQSAINTYQKNFNLKVTGKL 107

Query: 101 DAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAF 160
           D+ TL  + +PRCG PD+   +   + NG         Y+F    KP+W   K +L YAF
Sbjct: 108 DSSTLRQIVKPRCGNPDLI--DGVSEMNGGKILRTTEKYSFFPG-KPRWPKRKRDLTYAF 167

Query: 161 V--GSFPKQYKATVVGAMHSWSSKSGFKFTKG-SANKADVKISFEVGDHGDGHPFKEGSG 220
               +   + K     A   W+  +   FT+  S  +AD+ I F  G+HGDG PF    G
Sbjct: 168 APQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMG 227

Query: 221 ELGHAFAPEDGRLHFNAREAW--ADGAYPDKF-------DVGSVALHELGHILGLGHSSF 280
            L HA +P  G LH +  E W  ++G    +        D+ SVA+HE+GH+LGLGHSS 
Sbjct: 228 TLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSV 287

Query: 281 RNAVMWPSI-GPNFVMGLNDDDVNGLLALYNG 300
            +A+M+P+I G +  + L  DD+ G+  LY G
Sbjct: 288 EDAIMFPAISGGDRKVELAKDDIEGIQHLYGG 315

[Close]

BLAST of CmoCh04G028600 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 8.6e-32
Identity = 94/258 (36.43%), Postives = 131/258 (50.78%), Query Frame = 0


Query: 53  IHNVRAFLQRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPR 112
           I  ++  LQ+YGYL  N E+     D + E A+  YQK   L ++G  D++TL  +  PR
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPR 109

Query: 113 CGVPDVFINETSGDRNGDGDHPIGLHYAFINDLKPKWGAS-KYNLKYAFVGS------FP 172
           CG PD    +T+    G         Y +    +P+W       L YAF          P
Sbjct: 110 CGFPDDVEPKTAPFHTGK-------KYVYFPG-RPRWTRDVPLKLTYAFSQENLTPYLAP 169

Query: 173 KQYKATVVGAMHSWSSKSGFKFTKGSANK-ADVKISFEVGDHGDGHPFKEGSGELGHAFA 232
              +     A   W+S     F +      AD+KI F  GDHGDG PF    G L H F+
Sbjct: 170 TDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFS 229

Query: 233 PEDGRLHFNAREAWADGAYPDK----FDVGSVALHELGHILGLGHSSFRNAVMWPSIGP- 292
           PE+GRLH +  E WA     +K     D+ SVA+HE+GH+LGLGHSS ++A M+P++ P 
Sbjct: 230 PENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPR 289

Query: 293 NFVMGLNDDDVNGLLALY 298
           +  + LN DDV G+ +LY
Sbjct: 290 SKKVNLNMDDVVGVQSLY 295

[Close]

BLAST of CmoCh04G028600 vs. ExPASy TrEMBL
Match: A0A6J1H620 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460796 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.0e-165
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0


Query: 23  MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLE 82
           MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLE
Sbjct: 1   MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLE 60

Query: 83  FAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFI 142
           FAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFI
Sbjct: 61  FAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFI 120

Query: 143 NDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISFE 202
           NDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISFE
Sbjct: 121 NDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISFE 180

Query: 203 VGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHILG 262
           VGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHILG
Sbjct: 181 VGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHILG 240

Query: 263 LGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFREK 304
           LGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFREK
Sbjct: 241 LGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFREK 281

[Close]

BLAST of CmoCh04G028600 vs. ExPASy TrEMBL
Match: A0A6J1H7I1 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.0e-144
Identity = 250/302 (82.78%), Postives = 268/302 (88.74%), Query Frame = 0


Query: 1   MDLTSCLQLMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60
           MDL SCLQ+MFILVAAIAPHAIMARHLH HKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL
Sbjct: 1   MDLRSCLQVMFILVAAIAPHAIMARHLHIHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI 120
           QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI
Sbjct: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI 120

Query: 121 NETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWS 180
           NET GD NGD DHPIGLHY+F NDLKPKW ASKYNLKY F+GSFP+QYK TV+ AM SWS
Sbjct: 121 NETGGDGNGDRDHPIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVIQAMISWS 180

Query: 181 SKSGFKFTKGSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWAD 240
             S F F++GS +  DV+ISFEVGDHGDG PFK+ +G L HAFAPEDGRLHFNA+ AWAD
Sbjct: 181 FNSVFAFSEGSTDNTDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFNAQHAWAD 240

Query: 241 GAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGF 300
           GAYPDKFDVGSVALHELGHILGLGHS  R+AVMWPS+ PN    L+ DDV+G+ ALY+G 
Sbjct: 241 GAYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGISALYDGV 300

Query: 301 RE 303
            E
Sbjct: 301 EE 302

[Close]

BLAST of CmoCh04G028600 vs. ExPASy TrEMBL
Match: A0A6J1H5Y2 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460835 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.3e-136
Identity = 233/280 (83.21%), Postives = 255/280 (91.07%), Query Frame = 0


Query: 23  MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLE 82
           MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGN FDDNLE
Sbjct: 1   MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNAFDDNLE 60

Query: 83  FAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFI 142
           FA+KSYQKFFNLNVSGILDAETL LMSQPRCGVPDVFINETSGDR+ D DH IGL Y F 
Sbjct: 61  FALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDVFINETSGDRDRDWDHSIGLDYTFF 120

Query: 143 NDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISFE 202
           ++ KPKW ASKYNLKYAFVG+FP+QYKATV  A ++WSS +GF FT+GSA+  DVKISFE
Sbjct: 121 HEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVTGFTFTEGSADNTDVKISFE 180

Query: 203 VGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHILG 262
           VGDHGDG+PF+EGSG L HAFAPEDGRLH+NAREAWADGAYPDK+DVG+VALHELGH+LG
Sbjct: 181 VGDHGDGYPFEEGSGVLAHAFAPEDGRLHYNAREAWADGAYPDKYDVGTVALHELGHVLG 240

Query: 263 LGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFRE 303
           LGHS+FR AVMWP   PNFV GL++DDVNG++ALYNGF E
Sbjct: 241 LGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALYNGFEE 280

[Close]

BLAST of CmoCh04G028600 vs. ExPASy TrEMBL
Match: A0A6J1H7Q4 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.6e-132
Identity = 233/302 (77.15%), Postives = 255/302 (84.44%), Query Frame = 0


Query: 1   MDLTSCLQLMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60
           MDL SCLQLMFILVAAIAPHAIMARHLHTHK SSSIFPQHLVGSGKGHN+EGIHNVRAFL
Sbjct: 1   MDLKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVRAFL 60

Query: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI 120
           QRYGYLSTNV+TKGN FDDNLEFA+KSYQKFFNLNVSGILD ETLELMSQPRCGVPDVF 
Sbjct: 61  QRYGYLSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGVPDVF- 120

Query: 121 NETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWS 180
                           + Y F  + KPKW ASKYNLKYAFVG+FP+QYKATV  A ++WS
Sbjct: 121 ---------------RIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWS 180

Query: 181 SKSGFKFTKGSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWAD 240
           S +GF FT+GS +  DVKISFEVGDHGDG+PF EGSG L HAFAPEDGRLHFN R AWAD
Sbjct: 181 SVTGFTFTEGSVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWAD 240

Query: 241 GAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGF 300
           GAYP K+DVG+VALHELGH+LGLGHS+FR AVMWP   PNFV GL++DDVNG++ALYNGF
Sbjct: 241 GAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDDVNGIMALYNGF 286

Query: 301 RE 303
            +
Sbjct: 301 AD 286

[Close]

BLAST of CmoCh04G028600 vs. ExPASy TrEMBL
Match: A0A6J1BVR3 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 5.7e-79
Identity = 162/296 (54.73%), Postives = 199/296 (67.23%), Query Frame = 0


Query: 9   LMFILVAAIAPHAIMARHLHTH---KLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGY 68
           + FIL+AAIA H   AR LH H   K S  IF Q+L+GS KGHNI+GIH+V+ +L+RYGY
Sbjct: 13  ISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGY 72

Query: 69  LSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSG 128
            +    T+ N FDD+LE A+K+YQKFFNLNVSGILD ETL LMSQPRC VPD+F NETS 
Sbjct: 73  FT---HTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETST 132

Query: 129 DRN-GDGDH-PIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKS 188
            +  G  +H  +G HY F     PKW  SK  L+Y++   FP  +K  V  A+  WS+  
Sbjct: 133 RKGWGHEEHLHMGSHYLFF-PRTPKWPESKCRLRYSYYHDFPMAFKDPVNNALELWSAYL 192

Query: 189 GFKFTKGSANK--ADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADG 248
            F F +   +   AD+KISFE+G+HGDGHPF   SG L HAFAP DGR HFN  + W  G
Sbjct: 193 QFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVG 252

Query: 249 AYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALY 298
           A P+  DV SVA+HE+GH+LGLGHS    A+MWP I PN V GL  D+V G+ ALY
Sbjct: 253 ARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIKALY 304

[Close]

BLAST of CmoCh04G028600 vs. NCBI nr
Match: KAG6602459.1 (hypothetical protein SDJN03_07692, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 617.1 bits (1590), Expect = 8.2e-173
Identity = 297/303 (98.02%), Postives = 299/303 (98.68%), Query Frame = 0


Query: 1   MDLTSCLQLMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60
           MDLTSCLQLMFILVAA+APHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL
Sbjct: 1   MDLTSCLQLMFILVAAMAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI 120
           QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELM QPRCGVPDVFI
Sbjct: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMLQPRCGVPDVFI 120

Query: 121 NETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWS 180
           NETSGDRNGDGDH IGLHYAFINDLKPKWG SKYNLKYAFVGSFPKQYKATVVGAMHSWS
Sbjct: 121 NETSGDRNGDGDHLIGLHYAFINDLKPKWGTSKYNLKYAFVGSFPKQYKATVVGAMHSWS 180

Query: 181 SKSGFKFTKGSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWAD 240
           SKSGFKFTKGSANKADVKISFEVGDHGDGHPF+EGSGEL HAFAPEDGRLHFNAREAWAD
Sbjct: 181 SKSGFKFTKGSANKADVKISFEVGDHGDGHPFEEGSGELAHAFAPEDGRLHFNAREAWAD 240

Query: 241 GAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGF 300
           GAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGF
Sbjct: 241 GAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGF 300

Query: 301 REK 304
           REK
Sbjct: 301 REK 303

[Close]

BLAST of CmoCh04G028600 vs. NCBI nr
Match: XP_022959861.1 (metalloendoproteinase 1-like [Cucurbita moschata])

HSP 1 Score: 590.5 bits (1521), Expect = 8.2e-165
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0


Query: 23  MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLE 82
           MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLE
Sbjct: 1   MARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLE 60

Query: 83  FAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFI 142
           FAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFI
Sbjct: 61  FAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFI 120

Query: 143 NDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISFE 202
           NDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISFE
Sbjct: 121 NDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISFE 180

Query: 203 VGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHILG 262
           VGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHILG
Sbjct: 181 VGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHILG 240

Query: 263 LGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFREK 304
           LGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFREK
Sbjct: 241 LGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFREK 281

[Close]

BLAST of CmoCh04G028600 vs. NCBI nr
Match: KAG6602463.1 (hypothetical protein SDJN03_07696, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 529.3 bits (1362), Expect = 2.3e-146
Identity = 251/302 (83.11%), Postives = 274/302 (90.73%), Query Frame = 0


Query: 1   MDLTSCLQLMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60
           MDLTSCLQLMFILV  IAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL
Sbjct: 1   MDLTSCLQLMFILVTVIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI 120
           QRYGYL TNV+TKGN FDDNLEFA+KSYQKFFNLNVSGILDAETL LMSQPRCGVPDVFI
Sbjct: 61  QRYGYLRTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDVFI 120

Query: 121 NETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWS 180
           NETSGD +GD DH IGL Y F ++ KPKW ASKYNLKYAFVG+FP+QYKATV  A ++WS
Sbjct: 121 NETSGDGDGDWDHSIGLDYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWS 180

Query: 181 SKSGFKFTKGSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWAD 240
           S +GF FT+GSA+  DVKISFEVGDHGDG+PF+EGSG L HAFAPEDGRLH+NAREAWAD
Sbjct: 181 SVTGFTFTEGSADNTDVKISFEVGDHGDGYPFEEGSGVLAHAFAPEDGRLHYNAREAWAD 240

Query: 241 GAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGF 300
           GAYPDK+DVG+VALHELGH+LGLGHS+FR AVMWP   PNFV GL++DDVNG++ALYNGF
Sbjct: 241 GAYPDKYDVGTVALHELGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALYNGF 300

Query: 301 RE 303
            E
Sbjct: 301 EE 302

[Close]

BLAST of CmoCh04G028600 vs. NCBI nr
Match: XP_023544665.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.0 bits (1351), Expect = 4.3e-145
Identity = 250/302 (82.78%), Postives = 276/302 (91.39%), Query Frame = 0


Query: 1   MDLTSCLQLMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60
           MDLTSCLQ+MF+LVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL
Sbjct: 1   MDLTSCLQVMFVLVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI 120
           QRYGYLSTNVETKGN FDDNLEFA+KSYQKFFNLNVSGILDAETL LMSQPRCGVPDVFI
Sbjct: 61  QRYGYLSTNVETKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDVFI 120

Query: 121 NETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWS 180
           NETSG+  GD DH IGL Y F ++ KPKW ASKYNLKYAFVG+FP+QYKATV  A ++WS
Sbjct: 121 NETSGE--GDRDHSIGLDYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWS 180

Query: 181 SKSGFKFTKGSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWAD 240
           S +GF FT+GSA+  DVKISFEVGDHGDG+PF++GSG L HAFAPEDGRLH+NA+EAWAD
Sbjct: 181 SVTGFTFTEGSADNTDVKISFEVGDHGDGYPFEDGSGVLAHAFAPEDGRLHYNAQEAWAD 240

Query: 241 GAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGF 300
           GAYPDK+DVG+VALHELGH+LGLGHS+FR AVMWP   PNFV GL++DDVNG++ALYNGF
Sbjct: 241 GAYPDKYDVGTVALHELGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALYNGF 300

Query: 301 RE 303
            E
Sbjct: 301 EE 300

[Close]

BLAST of CmoCh04G028600 vs. NCBI nr
Match: XP_022959850.1 (metalloendoproteinase 1-like [Cucurbita moschata])

HSP 1 Score: 521.2 bits (1341), Expect = 6.1e-144
Identity = 250/302 (82.78%), Postives = 268/302 (88.74%), Query Frame = 0


Query: 1   MDLTSCLQLMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60
           MDL SCLQ+MFILVAAIAPHAIMARHLH HKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL
Sbjct: 1   MDLRSCLQVMFILVAAIAPHAIMARHLHIHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI 120
           QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI
Sbjct: 61  QRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFI 120

Query: 121 NETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWS 180
           NET GD NGD DHPIGLHY+F NDLKPKW ASKYNLKY F+GSFP+QYK TV+ AM SWS
Sbjct: 121 NETGGDGNGDRDHPIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVIQAMISWS 180

Query: 181 SKSGFKFTKGSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWAD 240
             S F F++GS +  DV+ISFEVGDHGDG PFK+ +G L HAFAPEDGRLHFNA+ AWAD
Sbjct: 181 FNSVFAFSEGSTDNTDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFNAQHAWAD 240

Query: 241 GAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGF 300
           GAYPDKFDVGSVALHELGHILGLGHS  R+AVMWPS+ PN    L+ DDV+G+ ALY+G 
Sbjct: 241 GAYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGISALYDGV 300

Query: 301 RE 303
            E
Sbjct: 301 EE 302

[Close]

BLAST of CmoCh04G028600 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 168.3 bits (425), Expect = 9.4e-42
Identity = 119/315 (37.78%), Postives = 157/315 (49.84%), Query Frame = 0


Query: 9   LMFILVAAIAPHAIMARHLHTHKLSSSIFPQ-------------HLVGSGKGHNIEGIHN 68
           L F+L+    P+   AR+    + S++   Q              LV    G ++ G+  
Sbjct: 13  LCFVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSE 72

Query: 69  VRAFLQRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGV 128
           ++ +L R+GY++   E   +VFD  LE AI  YQ+   L ++G LD  T+ LMS PRCGV
Sbjct: 73  LKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGV 132

Query: 129 PDVFINETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAFVGSFPKQY------K 188
            D     T    N D  H    HY + N  KPKW  ++  L YA   +    Y      K
Sbjct: 133 SD-----THMTINNDFLHTTA-HYTYFNG-KPKW--NRDTLTYAISKTHKLDYLTSEDVK 192

Query: 189 ATVVGAMHSWSSKSGFKFTK-GSANKADVKISFEVGDHGDGHPFKEGSGELGHAFAPEDG 248
                A   WSS     F +      AD+KI F  GDHGDG PF    G L HAFAPE+G
Sbjct: 193 TVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENG 252

Query: 249 RLHFNAREAW-----ADGAYPDKFDVGSVALHELGHILGLGHSSFRNAVMWPSIGPNF-V 298
           RLH +A E W       G+     D+ SVA HE+GH+LGLGHSS  +AVM+PS+ P    
Sbjct: 253 RLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKK 312

[Close]

BLAST of CmoCh04G028600 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 164.9 bits (416), Expect = 1.0e-40
Identity = 104/281 (37.01%), Postives = 150/281 (53.38%), Query Frame = 0


Query: 40  HLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNV---FDDNLEFAIKSYQKFFNLNV 99
           +  G   G N++G++ ++ + QR+GY+       GN    FDD L+ A++ YQ  FNLNV
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETF--SGNFTDDFDDILKAAVELYQTNFNLNV 105

Query: 100 SGILDAETLELMSQPRCGVPDVFINETS---GDR------NGDGDHPIGLHYAFINDLKP 159
           +G LDA T++ +  PRCG PDV +N TS   G R      N    H   +    +   +P
Sbjct: 106 TGELDALTIQHIVIPRCGNPDV-VNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEP 165

Query: 160 KWGASKYNLKYAFVGSFP--KQYKATVVGAMHSWSSKSGFKFT-KGSANKADVKISFEVG 219
           +W  ++ +L YAF    P  ++ K+    A   WS  +   FT   S + +D+ I F  G
Sbjct: 166 RWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 225

Query: 220 DHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKF-------DVGSVALHEL 279
           DHGDG PF    G L HAF+P  G+ H +A E W      D F       D+ SVA+HE+
Sbjct: 226 DHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEI 285

Query: 280 GHILGLGHSSFRNAVMWPSIGP-NFVMGLNDDDVNGLLALY 298
           GH+LGLGHSS   ++M+P+I      + L +DDV G+  LY
Sbjct: 286 GHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of CmoCh04G028600 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.4e-40
Identity = 101/272 (37.13%), Postives = 147/272 (54.04%), Query Frame = 0


Query: 41  LVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGIL 100
           L G   G NI G+  ++ + +R+GY++T      + FDD L+ AI +YQK FNL V+G L
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-FDDVLQSAINTYQKNFNLKVTGKL 107

Query: 101 DAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAFINDLKPKWGASKYNLKYAF 160
           D+ TL  + +PRCG PD+   +   + NG         Y+F    KP+W   K +L YAF
Sbjct: 108 DSSTLRQIVKPRCGNPDLI--DGVSEMNGGKILRTTEKYSFFPG-KPRWPKRKRDLTYAF 167

Query: 161 V--GSFPKQYKATVVGAMHSWSSKSGFKFTKG-SANKADVKISFEVGDHGDGHPFKEGSG 220
               +   + K     A   W+  +   FT+  S  +AD+ I F  G+HGDG PF    G
Sbjct: 168 APQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMG 227

Query: 221 ELGHAFAPEDGRLHFNAREAW--ADGAYPDKF-------DVGSVALHELGHILGLGHSSF 280
            L HA +P  G LH +  E W  ++G    +        D+ SVA+HE+GH+LGLGHSS 
Sbjct: 228 TLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSV 287

Query: 281 RNAVMWPSI-GPNFVMGLNDDDVNGLLALYNG 300
            +A+M+P+I G +  + L  DD+ G+  LY G
Sbjct: 288 EDAIMFPAISGGDRKVELAKDDIEGIQHLYGG 315

[Close]

BLAST of CmoCh04G028600 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 151.0 bits (380), Expect = 1.6e-36
Identity = 107/332 (32.23%), Postives = 167/332 (50.30%), Query Frame = 0


Query: 6   CLQLMFILVAAIAPHAIMARHLHTHKLSSSIFPQ--------------HLVGSGKGHNIE 65
           C+ ++F+L  A +P   ++   +T+  SS+I PQ              +  G   G   +
Sbjct: 5   CVFMVFLLFFAPSP---VSAGFYTN--SSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYD 64

Query: 66  GIHNVRAFLQRYGYLSTNVETKGNV---FDDNLEFAIKSYQKFFNLNVSGILDAETLELM 125
           G++ ++ + Q +GY+ T     GN    FDD L+ A++ YQ+ F LNV+G+LD  TL+ +
Sbjct: 65  GLYMLKQYFQHFGYI-TETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHV 124

Query: 126 SQPRCGVPDVFINETSGDRNGDGDHPIGL-----------HYAFINDLKPKWGASKYNLK 185
             PRCG PDV +N TS   +G     +             HY+F    +P+W  ++ +L 
Sbjct: 125 VIPRCGNPDV-VNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG-EPRWPRNRRDLT 184

Query: 186 YAF--VGSFPKQYKATVVGAMHSWSSKSGFKFTKGSA-NKADVKISFEVGDHGDGHPFKE 245
           YAF    +  ++ K+    A   W   +   FT+    + +D+ I F  G+HGDG PF  
Sbjct: 185 YAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDG 244

Query: 246 GSGELGHAFAPEDGRLHFNAREAW------ADG--AYPDKFDVGSVALHELGHILGLGHS 298
               L HAF+P  G  H +  E W       DG  +  +  D+ SVA+HE+GH+LGLGHS
Sbjct: 245 PMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHS 304

[Close]

BLAST of CmoCh04G028600 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 139.0 bits (349), Expect = 6.1e-33
Identity = 94/258 (36.43%), Postives = 131/258 (50.78%), Query Frame = 0


Query: 53  IHNVRAFLQRYGYLSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPR 112
           I  ++  LQ+YGYL  N E+     D + E A+  YQK   L ++G  D++TL  +  PR
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPR 109

Query: 113 CGVPDVFINETSGDRNGDGDHPIGLHYAFINDLKPKWGAS-KYNLKYAFVGS------FP 172
           CG PD    +T+    G         Y +    +P+W       L YAF          P
Sbjct: 110 CGFPDDVEPKTAPFHTGK-------KYVYFPG-RPRWTRDVPLKLTYAFSQENLTPYLAP 169

Query: 173 KQYKATVVGAMHSWSSKSGFKFTKGSANK-ADVKISFEVGDHGDGHPFKEGSGELGHAFA 232
              +     A   W+S     F +      AD+KI F  GDHGDG PF    G L H F+
Sbjct: 170 TDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFS 229

Query: 233 PEDGRLHFNAREAWADGAYPDK----FDVGSVALHELGHILGLGHSSFRNAVMWPSIGP- 292
           PE+GRLH +  E WA     +K     D+ SVA+HE+GH+LGLGHSS ++A M+P++ P 
Sbjct: 230 PENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPR 289

Query: 293 NFVMGLNDDDVNGLLALY 298
           +  + LN DDV G+ +LY
Sbjct: 290 SKKVNLNMDDVVGVQSLY 295

The following BLAST results are available for this feature:

BLAST of CmoCh04G028600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29136	6.4e-43	40.23	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O23507	1.3e-40	37.78	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]
O04529	1.5e-39	37.01	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q9ZUJ5	1.9e-39	37.13	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
Q8GWW6	8.6e-32	36.43	Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1	[more]

BLAST of CmoCh04G028600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H620	4.0e-165	100.00	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460796 PE=3 ...	[more]
A0A6J1H7I1	3.0e-144	82.78	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 ...	[more]
A0A6J1H5Y2	1.3e-136	83.21	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460835 PE=3 ...	[more]
A0A6J1H7Q4	2.6e-132	77.15	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 ...	[more]
A0A6J1BVR3	5.7e-79	54.73	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3...	[more]

BLAST of CmoCh04G028600 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6602459.1	8.2e-173	98.02	hypothetical protein SDJN03_07692, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022959861.1	8.2e-165	100.00	metalloendoproteinase 1-like [Cucurbita moschata]	[more]
KAG6602463.1	2.3e-146	83.11	hypothetical protein SDJN03_07696, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023544665.1	4.3e-145	82.78	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022959850.1	6.1e-144	82.78	metalloendoproteinase 1-like [Cucurbita moschata]	[more]

BLAST of CmoCh04G028600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16640.1	9.4e-42	37.78	Matrixin family protein	[more]
AT1G70170.1	1.0e-40	37.01	matrix metalloproteinase	[more]
AT1G59970.1	1.4e-40	37.13	Matrixin family protein	[more]
AT1G24140.1	1.6e-36	32.23	Matrixin family protein	[more]
AT2G45040.1	6.1e-33	36.43	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 172..187 score: 25.46 coord: 252..277 score: 55.91 coord: 108..121 score: 59.23 coord: 195..223 score: 48.95 coord: 285..298 score: 46.17
IPR021190	Peptidase M10A	PIRSF	PIRSF001191	MMP_stromelysin	coord: 18..303 e-value: 2.9E-52 score: 175.8
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 145..299 e-value: 6.0E-23 score: 92.2
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 51..108 e-value: 8.2E-13 score: 48.3
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 153..297 e-value: 9.7E-34 score: 116.5
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 42..300 e-value: 4.5E-66 score: 224.8
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 25..297
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 25..297
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 148..298
IPR021158	Peptidase M10A, cysteine switch, zinc binding site	PROSITE	PS00546	CYSTEINE_SWITCH	coord: 111..118
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 46..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G028600.1	CmoCh04G028600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity