CmoCh04G027000 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G027000
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionplasma membrane intrinsic protein 2
LocationCmo_Chr04: 19632374 .. 19635733 (+)
RNA-Seq ExpressionCmoCh04G027000
SyntenyCmoCh04G027000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATGGCCGTAAGAGTAAAGATGCAAACGCTATCACTTCTAAGCAACGTTCTTCTAATAAACTGTAAATGCATACACAGGGCAAAGACGTTAAAAGGTTACCGCCATTGATTATTTATTTGGAGCATGAAAGGAATTTGTCGTTACTGTAGTAAGTGGTATGCACAGTGAAATCACTGTTCTGAGTGGGTCAGAGCGACAAAGGTACGGAGCATAGCAATGGCAAGTGGGTGGGCGGTGTGCCCAACTCAGCCGGAGCCGCTGAAACTCACTGCTCCTTTTCTCTTTTCATCCTTCAATTTCCTCTTATTAAATGCAGCTCTTCCCTCCCCAACTTTGCACCATATTCAAATCAAAATCCATCGCCGGAAATTGATTTTCTCTCTCCTAGGGCTTCGATTTTCGATTTCTCTGTCATTATGGCTAAGAATGCAGCGGAACGCGGCTCGTTCTCGGCGAAGGACTACCACGATCCACCACCGGCGCCGCTCATCGATGCCGTGGAGATTACGAAATGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTCTGCTTTTCCTGTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATATCGGCAACAAGAACGTTGATCCTTGCGGTGGCGTCGGGATTCTTGGCATTGCCTGGGCGTTTGGTGGAATGATCTTCGTTCTTGTTTACTGTACCGCTGGAATTTCAGGTTCTTCTTTCTCCGTTTCTTCGGCTTAAAGTTTAAGTATTGAAATTCTTTTTCCAGTTTTTCTTTTTGGTTGAGAGTTCATGATATTATCATGCACTTTGTTTACGTTATTCCATTTGGCGCTTACACATTATTTATTTATTATTATTATTATTATTTTGGTGGAAAAATCATTTTTAAATCAATATTTTATTGGTTTTAGATCCTCATTTTAAAATTTATACCCAGTTGAAAATGTAAACAAAAATATCAAAATTCGTATGTAATAGAAATATTTATAAAAATTTTGAAAAAAAAAATACTGATAGAAATTCCTATAAATATCATGTTTTTATTTTATTTTATTTTTTAAAATTATGCAAATTTGAATAAAAGTGAAACAAAATCCAAATATTCCTTCTCATTTATTAAACTATAGTTTATATTTAGGAAATTATATATTAATTTTATTATTATCTTTTTCCGTTTAAAGAAAAGCTTTTAATATTTCAAGTTTTTTTCCGTGTATTATTGTAACGGTCGTATTTAATCTCCAATATAAATATATAATATTGTGATGTCGTTGTGAAGAGAACAAAACATTCTTTATAAGAGCGTGGAAACTTTCTCCGAGCAAGTGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAACGAAGACAATATTTGCTAGCGGTAGGCTTGGGTCGTTACAAATATTTTAATTATTTAGCCTATGTTCGGGTTGGTCATAATTTTTAATTTTGAGTTTATTTTTTTTAGAAAAATGAAAGAAAAGAAAGTTAGTGTTGACTTGAGGAGGATGTGCAGGTGGGCACATAAATCCAGCAGTGACATTTGGGCTTTTGTTGGCCCGAAAGGTGTCACTTGTCCGGGCCGTGATGTACATGGTGGCCCAATGCTTAGGGGCCATTTGCGGAGTTGGGCTCGTGAAGTCCTTCCAGAAGGCCCACTTCCAGAAGTACGGCGGTGGGGCAAATGGGCTCGCCGACGGCTACACCGTCGGCGTTGGACTGGCGGCGGAGATCGTGGGGACGTTTGTGTTGGTGTATACAGTCTTCTCTGCTACAGATCCCAAACGAAGCGCAAGAGATTCCCACGTTCCTGTATGCATCTTTAATTTTAATCTCATAAATTATATTTAAAAGATTAAAAATATTTATACTTTAATATTTTTTATAATTTTAAATATTAAATTTTATTATTTCTACTTTCAATAAAAAGTTAAAATTAGTTTATTCTTCAATTTTGTAATAAAAAATAAATAATAAATAATCTCTTTTTTTTGGGTATTTTATTTTAATGGTCAGTTAATTTTTTGTTATTGAAAGAATTATAAAGTGGGTTCATGTGTTAGGCACCAACTTTCACCTGTAGATGCCTCTTTCAGAACTTACTTTCTTACGGATAATAATAATAATAAATAAATAAATAAATAAATAAATTGCTGTTATCTTTTTGATCAAATGAAAGTTATGAACTTTATTTTATTTGGCTTCACATGCATACAAATCCTTATCTTCTTTTTTTGTCCTTGAAGGTTTTGGCGCCACTCCCAATTGGATTTGCAGTGTTCATAGTTCACCTGGCCACCATTCCAGTCACTGGCACTGGAATCAACCCTGCAAGGAGCTTGGGATCTGCTGTTATTTTCAACCACCAAAACACTTGGAATAATCATGTTAGTTTCTATCTTTATCTACGGTAAAGTTCTATTAATTTAAACGGAACTTAACTAGTTAAGGTAGGTACTCTAACTAATAGACTAGAAGCTCGAATCCCTCTATTACCACATGTTGTTTATACTCTGACTAATAGACTAGAAGCTTGAGTCCCTCCATTCCCATATGTTTATACCCTGACTAAAGACGAGAAGCTGAGTCCCTCCATTTCCACATATTGTTTCGACTCTGATTAATAGGCTAGAAGTTCAAGTCTCTCCATCCCACATATTATTTCTACCCTGACTAATAGACTAGAAGATCGAGTCCCTCCATTCTCACATATTGTTTATACTCTGACTAATAGACTAGAAGTTCGAGTCCCTCCATTCTCACATGTTGTTTATACTTTGACTAATAGACTACAAGCTCGAGTCCCTTCATTCCTATATGGTGTTGAACTAAAAAAGAAACTTCATCTAGACTTCTTGATTCGATAACAATATCATAAATAATCTAACATTTCAAAAAGAGAAACGTTGATGACTCAAATACTTGGTTTTGGGAACTAATTGGTATGGATATTTGTTTGCAGTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCTTTCTACCACCAATTCATCTTGCGAGCAGGTGCTGTGAAGGCTCTTGGTTCATTTAGAAGCAACCCAAATGTGTGATGATCATAACGCTGCCTTTAATTAGCTCCTGGAGATTTAAGAACCAGATGTTAACACTAGTACTTGCATTTTGAGTTTTTATTTCCCATGTTTGCTTGAGACTTTCATGAATCTACTAGTTCTTTCTTTTGCTATTGGAATGGTTAAAAATAGTTTTTCCTTCTCGGAAATGGCAAAGTTTGATATTGAGTCGGGAGAAAAATGCATAAAAACACAAACTGTATCGCCTATTCCACAAGCTAATCCCAAC

mRNA sequence

ATGGCAATGGCCGTAAGAGTAAAGATGCAAACGCTATCACTTCTAAGCAACGTTCTTCTAATAAACTTAAGTGGTATGCACAGTGAAATCACTGTTCTGAGTGGGTCAGAGCGACAAAGCTCTTCCCTCCCCAACTTTGCACCATATTCAAATCAAAATCCATCGCCGGAAATTGATTTTCTCTCTCCTAGGGCTTCGATTTTCGATTTCTCTGTCATTATGGCTAAGAATGCAGCGGAACGCGGCTCGTTCTCGGCGAAGGACTACCACGATCCACCACCGGCGCCGCTCATCGATGCCGTGGAGATTACGAAATGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTCTGCTTTTCCTGTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATATCGGCAACAAGAACGTTGATCCTTGCGGTGGCGTCGGGATTCTTGGCATTGCCTGGGCGTTTGGTGGAATGATCTTCGTTCTTGTTTACTGTACCGCTGGAATTTCAGGTGGGCACATAAATCCAGCAGTGACATTTGGGCTTTTGTTGGCCCGAAAGGTGTCACTTGTCCGGGCCGTGATGTACATGGTGGCCCAATGCTTAGGGGCCATTTGCGGAGTTGGGCTCGTGAAGTCCTTCCAGAAGGCCCACTTCCAGAAGTACGGCGGTGGGGCAAATGGGCTCGCCGACGGCTACACCGTCGGCGTTGGACTGGCGGCGGAGATCGTGGGGACGTTTGTGTTGGTGTATACAGTCTTCTCTGCTACAGATCCCAAACGAAGCGCAAGAGATTCCCACGTTCCTGTTTTGGCGCCACTCCCAATTGGATTTGCAGTGTTCATAGTTCACCTGGCCACCATTCCAGTCACTGGCACTGGAATCAACCCTGCAAGGAGCTTGGGATCTGCTGTTATTTTCAACCACCAAAACACTTGGAATAATCATTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCTTTCTACCACCAATTCATCTTGCGAGCAGGTGCTGTGAAGGCTCTTGGTTCATTTAGAAGCAACCCAAATGTGTGATGATCATAACGCTGCCTTTAATTAGCTCCTGGAGATTTAAGAACCAGATGTTAACACTAGTACTTGCATTTTGAGTTTTTATTTCCCATGTTTGCTTGAGACTTTCATGAATCTACTAGTTCTTTCTTTTGCTATTGGAATGGTTAAAAATAGTTTTTCCTTCTCGGAAATGGCAAAGTTTGATATTGAGTCGGGAGAAAAATGCATAAAAACACAAACTGTATCGCCTATTCCACAAGCTAATCCCAAC

Coding sequence (CDS)

ATGGCAATGGCCGTAAGAGTAAAGATGCAAACGCTATCACTTCTAAGCAACGTTCTTCTAATAAACTTAAGTGGTATGCACAGTGAAATCACTGTTCTGAGTGGGTCAGAGCGACAAAGCTCTTCCCTCCCCAACTTTGCACCATATTCAAATCAAAATCCATCGCCGGAAATTGATTTTCTCTCTCCTAGGGCTTCGATTTTCGATTTCTCTGTCATTATGGCTAAGAATGCAGCGGAACGCGGCTCGTTCTCGGCGAAGGACTACCACGATCCACCACCGGCGCCGCTCATCGATGCCGTGGAGATTACGAAATGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTCTGCTTTTCCTGTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATATCGGCAACAAGAACGTTGATCCTTGCGGTGGCGTCGGGATTCTTGGCATTGCCTGGGCGTTTGGTGGAATGATCTTCGTTCTTGTTTACTGTACCGCTGGAATTTCAGGTGGGCACATAAATCCAGCAGTGACATTTGGGCTTTTGTTGGCCCGAAAGGTGTCACTTGTCCGGGCCGTGATGTACATGGTGGCCCAATGCTTAGGGGCCATTTGCGGAGTTGGGCTCGTGAAGTCCTTCCAGAAGGCCCACTTCCAGAAGTACGGCGGTGGGGCAAATGGGCTCGCCGACGGCTACACCGTCGGCGTTGGACTGGCGGCGGAGATCGTGGGGACGTTTGTGTTGGTGTATACAGTCTTCTCTGCTACAGATCCCAAACGAAGCGCAAGAGATTCCCACGTTCCTGTTTTGGCGCCACTCCCAATTGGATTTGCAGTGTTCATAGTTCACCTGGCCACCATTCCAGTCACTGGCACTGGAATCAACCCTGCAAGGAGCTTGGGATCTGCTGTTATTTTCAACCACCAAAACACTTGGAATAATCATTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCTTTCTACCACCAATTCATCTTGCGAGCAGGTGCTGTGAAGGCTCTTGGTTCATTTAGAAGCAACCCAAATGTGTGA

Protein sequence

MAMAVRVKMQTLSLLSNVLLINLSGMHSEITVLSGSERQSSSLPNFAPYSNQNPSPEIDFLSPRASIFDFSVIMAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Homology
BLAST of CmoCh04G027000 vs. ExPASy Swiss-Prot
Match: Q9SV31 (Probable aquaporin PIP2-5 OS=Arabidopsis thaliana OX=3702 GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 7.9e-133
Identity = 231/281 (82.21%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 80  ERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDH 139
           ++ SFS KDY DPPP PL DA E+ KWSFYRALIAEFIATLLFLYVT++TVIGYKSQTD 
Sbjct: 8   DKRSFSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDP 67

Query: 140 IGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAV 199
               N D C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLLLARKV+LVRAV
Sbjct: 68  A--LNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVTLVRAV 127

Query: 200 MYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVF 259
           MYMVAQCLGAICGV LVK+FQ A+F +YGGGANGL+DGY++G G+AAEI+GTFVLVYTVF
Sbjct: 128 MYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVYTVF 187

Query: 260 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWN 319
           SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIP+TGTGINPARSLG+A+I+N    W+
Sbjct: 188 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPARSLGAAIIYNKDKAWD 247

Query: 320 NHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           +HWIFWVGPF GAAIAAFYHQF+LRAGA+KALGSFRS P+V
Sbjct: 248 HHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALGSFRSQPHV 286

BLAST of CmoCh04G027000 vs. ExPASy Swiss-Prot
Match: Q8H5N9 (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.4e-129
Identity = 225/276 (81.52%), Postives = 251/276 (90.94%), Query Frame = 0

Query: 82  GSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHIG 141
           G F+AKDY DPPPAPLIDA E+  WS YRA+IAEFIATLLFLY+TV TVIGYK QTD   
Sbjct: 14  GEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASA 73

Query: 142 NKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMY 201
           +     CGGVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKVSLVRA++Y
Sbjct: 74  SGADAACGGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILY 133

Query: 202 MVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSA 261
           +VAQCLGAICGVGLVK+FQ A+F +YGGGAN LA GY+ G GLAAEI+GTFVLVYTVFSA
Sbjct: 134 IVAQCLGAICGVGLVKAFQSAYFNRYGGGANTLAAGYSKGTGLAAEIIGTFVLVYTVFSA 193

Query: 262 TDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWNNH 321
           TDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS+G+AVIFN++  W+NH
Sbjct: 194 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSIGAAVIFNNEKAWHNH 253

Query: 322 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 358
           WIFWVGPF+GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 254 WIFWVGPFVGAAIAAFYHQYILRAGAIKALGSFRSN 289

BLAST of CmoCh04G027000 vs. ExPASy Swiss-Prot
Match: Q84RL7 (Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 458.4 bits (1178), Expect = 7.6e-128
Identity = 225/282 (79.79%), Postives = 250/282 (88.65%), Query Frame = 0

Query: 76  KNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKS 135
           ++ A  G F+AKDY DPPPAPLIDA E+  WS YRA+IAEFIATLLFLY+TV TVIGYK 
Sbjct: 8   ESGAGGGEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKH 67

Query: 136 QTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSL 195
           QTD   +     CGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LARKVSL
Sbjct: 68  QTDASASGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSL 127

Query: 196 VRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLV 255
           VRA++Y+VAQCLGAICGVGLVK+FQ A+F +YGGGAN LA GY+ G GL AEI+GTFVLV
Sbjct: 128 VRALLYIVAQCLGAICGVGLVKAFQSAYFDRYGGGANSLASGYSRGTGLGAEIIGTFVLV 187

Query: 256 YTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQ 315
           YTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIPVTGTGINPARSLG+AVI+N  
Sbjct: 188 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYNKD 247

Query: 316 NTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 358
             W++HWIFWVGP +GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 248 KPWDDHWIFWVGPLVGAAIAAFYHQYILRAGAIKALGSFRSN 289

BLAST of CmoCh04G027000 vs. ExPASy Swiss-Prot
Match: Q9ATM8 (Aquaporin PIP2-2 OS=Zea mays OX=4577 GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 2.9e-127
Identity = 226/286 (79.02%), Postives = 251/286 (87.76%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           + ++ A  G F+AKDY DPPPAPL+DA E+  WS YRA+IAEFIATLLFLYVTV TVIGY
Sbjct: 6   VVQSGAGGGEFAAKDYTDPPPAPLVDAAELGSWSLYRAVIAEFIATLLFLYVTVATVIGY 65

Query: 134 KSQTD-HIGNKNVD-PCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 193
           K QTD        D  CGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LAR
Sbjct: 66  KHQTDASASGAGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLAR 125

Query: 194 KVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGT 253
           KVSLVRA++YMVAQCLGA+CGVGLVK+FQ A+F +YGGGAN LA GY+ G GL AEIVGT
Sbjct: 126 KVSLVRALLYMVAQCLGAVCGVGLVKAFQSAYFDRYGGGANSLASGYSRGAGLGAEIVGT 185

Query: 254 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVI 313
           FVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIPVTGTGINPARSLG+AV+
Sbjct: 186 FVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVV 245

Query: 314 FNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 358
           +N    W++HWIFWVGP +GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 246 YNKDKPWDDHWIFWVGPLLGAAIAAFYHQYILRAGAIKALGSFRSN 291

BLAST of CmoCh04G027000 vs. ExPASy Swiss-Prot
Match: P30302 (Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.6e-125
Identity = 224/287 (78.05%), Postives = 245/287 (85.37%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAK+      F  +DY DPPP P  DA E+TKWS YRA+IAEF+ATLLFLYVTVLTVIGY
Sbjct: 1   MAKDVEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           K Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KIQSDTKAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SL+RAV+YMVAQCLGAICGVG VK+FQ +H+  YGGGAN LADGY  G GLAAEI+GTFV
Sbjct: 121 SLIRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVIFN
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
               W++HWIFWVGPFIGA IAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 KSKPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of CmoCh04G027000 vs. ExPASy TrEMBL
Match: A0A6J1JQ27 (probable aquaporin PIP2-5 OS=Cucurbita maxima OX=3661 GN=LOC111487288 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.1e-160
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of CmoCh04G027000 vs. ExPASy TrEMBL
Match: A0A6J1GSM7 (probable aquaporin PIP2-5 OS=Cucurbita moschata OX=3662 GN=LOC111456599 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.1e-160
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of CmoCh04G027000 vs. ExPASy TrEMBL
Match: A0A6J1BY62 (probable aquaporin PIP2-5 OS=Momordica charantia OX=3673 GN=LOC111006527 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 3.0e-151
Identity = 270/287 (94.08%), Postives = 278/287 (96.86%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAKNAAERGSFS KDYHDPPPAPLIDAVEITKWSFYRALIAEFIAT+LFLY+TVLTVIGY
Sbjct: 1   MAKNAAERGSFSGKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATMLFLYITVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           KSQTDH  NKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTANKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SLVRAVMYMVAQCLGAI GVGLVK+FQKAHFQKYGGGANGLADGYT GVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKYGGGANGLADGYTTGVGLAAEIVGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAV +VHLATIP+TGTGINPARSLGSAVI N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVLMVHLATIPITGTGINPARSLGSAVILN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
            Q TWN+HWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 DQKTWNDHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

BLAST of CmoCh04G027000 vs. ExPASy TrEMBL
Match: A0A5B6ZA79 (Putative aquaporin PIP2-5 OS=Davidia involucrata OX=16924 GN=Din_009434 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.3e-138
Identity = 251/289 (86.85%), Postives = 270/289 (93.43%), Query Frame = 0

Query: 74  MAKN--AAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVI 133
           MAK+   AE+GSFSAKDYHDPPPAPLID  E+TKWSFYRALIAEFIATLLFLY+TVLTVI
Sbjct: 1   MAKDVGVAEQGSFSAKDYHDPPPAPLIDPDELTKWSFYRALIAEFIATLLFLYITVLTVI 60

Query: 134 GYKSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 193
           GYKSQTD    K  DPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LAR
Sbjct: 61  GYKSQTDPA--KQADPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLAR 120

Query: 194 KVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGT 253
           KVSLVRAVMYMVAQCLGAICGVGLVK+FQK ++++YGGGAN LADGY+ GVGLAAEI+GT
Sbjct: 121 KVSLVRAVMYMVAQCLGAICGVGLVKAFQKPYYKRYGGGANELADGYSTGVGLAAEIIGT 180

Query: 254 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVI 313
           FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLG+AVI
Sbjct: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVI 240

Query: 314 FNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           +N    W++HW+FWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 YNQSKAWDDHWMFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

BLAST of CmoCh04G027000 vs. ExPASy TrEMBL
Match: A0A067LAW1 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_20043 PE=3 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 2.2e-138
Identity = 248/287 (86.41%), Postives = 267/287 (93.03%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           M K+ AE+GSFSAKDYHDPPPAPLIDA E+TKWSFYRALIAEFIATLLFLY+TVLTVIGY
Sbjct: 1   MVKDVAEQGSFSAKDYHDPPPAPLIDAEELTKWSFYRALIAEFIATLLFLYITVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           KSQTD    KN D CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KSQTDPA--KNADSCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SLVRAVMYMVAQ LGAICGVGLVK+FQ A++++YGGGAN LADGY+ GVGL AEI+GTFV
Sbjct: 121 SLVRAVMYMVAQSLGAICGVGLVKAFQSAYYKRYGGGANTLADGYSTGVGLGAEIIGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLG+AVI+N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           H   W++ WIFWVGPF+GAAIAAFYHQFILRAGAVKALGSFRSNP V
Sbjct: 241 HDKAWDDQWIFWVGPFLGAAIAAFYHQFILRAGAVKALGSFRSNPTV 285

BLAST of CmoCh04G027000 vs. NCBI nr
Match: XP_022954319.1 (probable aquaporin PIP2-5 [Cucurbita moschata] >XP_022990425.1 probable aquaporin PIP2-5 [Cucurbita maxima] >KAG6602317.1 putative aquaporin PIP2-5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033000.1 putative aquaporin PIP2-5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 575.1 bits (1481), Expect = 4.3e-160
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of CmoCh04G027000 vs. NCBI nr
Match: XP_023540772.1 (probable aquaporin PIP2-5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567.4 bits (1461), Expect = 8.9e-158
Identity = 284/287 (98.95%), Postives = 284/287 (98.95%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           KSQTDH GNKN D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of CmoCh04G027000 vs. NCBI nr
Match: XP_022134214.1 (probable aquaporin PIP2-5 [Momordica charantia])

HSP 1 Score: 544.7 bits (1402), Expect = 6.2e-151
Identity = 270/287 (94.08%), Postives = 278/287 (96.86%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAKNAAERGSFS KDYHDPPPAPLIDAVEITKWSFYRALIAEFIAT+LFLY+TVLTVIGY
Sbjct: 1   MAKNAAERGSFSGKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATMLFLYITVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           KSQTDH  NKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTANKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SLVRAVMYMVAQCLGAI GVGLVK+FQKAHFQKYGGGANGLADGYT GVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKYGGGANGLADGYTTGVGLAAEIVGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAV +VHLATIP+TGTGINPARSLGSAVI N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVLMVHLATIPITGTGINPARSLGSAVILN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
            Q TWN+HWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 DQKTWNDHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

BLAST of CmoCh04G027000 vs. NCBI nr
Match: KDP44363.1 (hypothetical protein JCGZ_20043 [Jatropha curcas])

HSP 1 Score: 501.9 bits (1291), Expect = 4.6e-138
Identity = 248/287 (86.41%), Postives = 267/287 (93.03%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           M K+ AE+GSFSAKDYHDPPPAPLIDA E+TKWSFYRALIAEFIATLLFLY+TVLTVIGY
Sbjct: 1   MVKDVAEQGSFSAKDYHDPPPAPLIDAEELTKWSFYRALIAEFIATLLFLYITVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           KSQTD    KN D CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KSQTDPA--KNADSCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SLVRAVMYMVAQ LGAICGVGLVK+FQ A++++YGGGAN LADGY+ GVGL AEI+GTFV
Sbjct: 121 SLVRAVMYMVAQSLGAICGVGLVKAFQSAYYKRYGGGANTLADGYSTGVGLGAEIIGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLG+AVI+N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           H   W++ WIFWVGPF+GAAIAAFYHQFILRAGAVKALGSFRSNP V
Sbjct: 241 HDKAWDDQWIFWVGPFLGAAIAAFYHQFILRAGAVKALGSFRSNPTV 285

BLAST of CmoCh04G027000 vs. NCBI nr
Match: XP_038886208.1 (probable aquaporin PIP2-5 [Benincasa hispida])

HSP 1 Score: 501.5 bits (1290), Expect = 6.0e-138
Identity = 250/277 (90.25%), Postives = 260/277 (93.86%), Query Frame = 0

Query: 84  FSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHIGNK 143
           F AKDY+DPPP PLID  EI KWSFYRALIAEF+ATLLFLYVTVLTVIGYKS+TD +G K
Sbjct: 33  FMAKDYYDPPPVPLIDGEEIRKWSFYRALIAEFMATLLFLYVTVLTVIGYKSETDGMGKK 92

Query: 144 NVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMYMV 203
           NVD CG VGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLL RKVSLVRAVMYMV
Sbjct: 93  NVDSCGEVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLGRKVSLVRAVMYMV 152

Query: 204 AQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSATD 263
           AQCLGAI GVGLVK+FQKAHFQKYGGGANGL+D YT  VGLAAEIVGTFVLVYTVFSATD
Sbjct: 153 AQCLGAISGVGLVKAFQKAHFQKYGGGANGLSDDYTASVGLAAEIVGTFVLVYTVFSATD 212

Query: 264 PKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWNNHWI 323
           PKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLGSAVIFN Q  WNNHWI
Sbjct: 213 PKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVIFNDQKAWNNHWI 272

Query: 324 FWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           FWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 273 FWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 309

BLAST of CmoCh04G027000 vs. TAIR 10
Match: AT3G54820.1 (plasma membrane intrinsic protein 2;5 )

HSP 1 Score: 474.9 bits (1221), Expect = 5.6e-134
Identity = 231/281 (82.21%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 80  ERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDH 139
           ++ SFS KDY DPPP PL DA E+ KWSFYRALIAEFIATLLFLYVT++TVIGYKSQTD 
Sbjct: 8   DKRSFSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDP 67

Query: 140 IGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAV 199
               N D C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLLLARKV+LVRAV
Sbjct: 68  A--LNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVTLVRAV 127

Query: 200 MYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVF 259
           MYMVAQCLGAICGV LVK+FQ A+F +YGGGANGL+DGY++G G+AAEI+GTFVLVYTVF
Sbjct: 128 MYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVYTVF 187

Query: 260 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWN 319
           SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIP+TGTGINPARSLG+A+I+N    W+
Sbjct: 188 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPARSLGAAIIYNKDKAWD 247

Query: 320 NHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           +HWIFWVGPF GAAIAAFYHQF+LRAGA+KALGSFRS P+V
Sbjct: 248 HHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALGSFRSQPHV 286

BLAST of CmoCh04G027000 vs. TAIR 10
Match: AT2G37180.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 450.7 bits (1158), Expect = 1.1e-126
Identity = 224/287 (78.05%), Postives = 245/287 (85.37%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAK+      F  +DY DPPP P  DA E+TKWS YRA+IAEF+ATLLFLYVTVLTVIGY
Sbjct: 1   MAKDVEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           K Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KIQSDTKAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SL+RAV+YMVAQCLGAICGVG VK+FQ +H+  YGGGAN LADGY  G GLAAEI+GTFV
Sbjct: 121 SLIRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVIFN
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
               W++HWIFWVGPFIGA IAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 KSKPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of CmoCh04G027000 vs. TAIR 10
Match: AT2G37170.1 (plasma membrane intrinsic protein 2 )

HSP 1 Score: 450.3 bits (1157), Expect = 1.5e-126
Identity = 222/287 (77.35%), Postives = 247/287 (86.06%), Query Frame = 0

Query: 74  MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 133
           MAK+      F  +DY DPPP P  DA E+TKWS YRA+IAEF+ATLLFLY+TVLTVIGY
Sbjct: 1   MAKDVEGPEGFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY 60

Query: 134 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 193
           K Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KIQSDTKAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 194 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 253
           SL+RAV+YMVAQCLGAICGVG VK+FQ +++ +YGGGAN LADGY  G GLAAEI+GTFV
Sbjct: 121 SLIRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFV 180

Query: 254 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 313
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVI+N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYN 240

Query: 314 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
               W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 KSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of CmoCh04G027000 vs. TAIR 10
Match: AT3G53420.1 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 446.4 bits (1147), Expect = 2.1e-125
Identity = 222/289 (76.82%), Postives = 249/289 (86.16%), Query Frame = 0

Query: 74  MAKN--AAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVI 133
           MAK+  A     F  +DY DPPPAP ID  E+ KWSFYRA+IAEF+ATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 134 GYKSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 193
           GYK Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LAR
Sbjct: 61  GYKIQSDTDAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLAR 120

Query: 194 KVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGT 253
           KVSL RA++Y++AQCLGAICGVG VK+FQ +++ +YGGGAN LADGY+ G GLAAEI+GT
Sbjct: 121 KVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGT 180

Query: 254 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVI 313
           FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVI
Sbjct: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI 240

Query: 314 FNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           +N    W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 YNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

BLAST of CmoCh04G027000 vs. TAIR 10
Match: AT3G53420.2 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 446.4 bits (1147), Expect = 2.1e-125
Identity = 222/289 (76.82%), Postives = 249/289 (86.16%), Query Frame = 0

Query: 74  MAKN--AAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVI 133
           MAK+  A     F  +DY DPPPAP ID  E+ KWSFYRA+IAEF+ATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 134 GYKSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 193
           GYK Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LAR
Sbjct: 61  GYKIQSDTDAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLAR 120

Query: 194 KVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGT 253
           KVSL RA++Y++AQCLGAICGVG VK+FQ +++ +YGGGAN LADGY+ G GLAAEI+GT
Sbjct: 121 KVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGT 180

Query: 254 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVI 313
           FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVI
Sbjct: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI 240

Query: 314 FNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 361
           +N    W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 YNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SV317.9e-13382.21Probable aquaporin PIP2-5 OS=Arabidopsis thaliana OX=3702 GN=PIP2-5 PE=1 SV=1[more]
Q8H5N91.4e-12981.52Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP2-1 PE=... [more]
Q84RL77.6e-12879.79Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2[more]
Q9ATM82.9e-12779.02Aquaporin PIP2-2 OS=Zea mays OX=4577 GN=PIP2-2 PE=2 SV=1[more]
P303021.6e-12578.05Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JQ272.1e-160100.00probable aquaporin PIP2-5 OS=Cucurbita maxima OX=3661 GN=LOC111487288 PE=3 SV=1[more]
A0A6J1GSM72.1e-160100.00probable aquaporin PIP2-5 OS=Cucurbita moschata OX=3662 GN=LOC111456599 PE=3 SV=... [more]
A0A6J1BY623.0e-15194.08probable aquaporin PIP2-5 OS=Momordica charantia OX=3673 GN=LOC111006527 PE=3 SV... [more]
A0A5B6ZA791.3e-13886.85Putative aquaporin PIP2-5 OS=Davidia involucrata OX=16924 GN=Din_009434 PE=3 SV=... [more]
A0A067LAW12.2e-13886.41Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_20043 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022954319.14.3e-160100.00probable aquaporin PIP2-5 [Cucurbita moschata] >XP_022990425.1 probable aquapori... [more]
XP_023540772.18.9e-15898.95probable aquaporin PIP2-5 [Cucurbita pepo subsp. pepo][more]
XP_022134214.16.2e-15194.08probable aquaporin PIP2-5 [Momordica charantia][more]
KDP44363.14.6e-13886.41hypothetical protein JCGZ_20043 [Jatropha curcas][more]
XP_038886208.16.0e-13890.25probable aquaporin PIP2-5 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G54820.15.6e-13482.21plasma membrane intrinsic protein 2;5 [more]
AT2G37180.11.1e-12678.05Aquaporin-like superfamily protein [more]
AT2G37170.11.5e-12677.35plasma membrane intrinsic protein 2 [more]
AT3G53420.12.1e-12576.82plasma membrane intrinsic protein 2A [more]
AT3G53420.22.1e-12576.82plasma membrane intrinsic protein 2A [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 322..342
score: 50.21
coord: 160..184
score: 62.61
coord: 197..216
score: 50.83
coord: 282..304
score: 56.93
coord: 246..264
score: 53.95
coord: 110..129
score: 45.9
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 114..339
e-value: 1.7E-63
score: 212.4
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 102..339
e-value: 2.8E-82
score: 275.8
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 110..342
e-value: 1.8779E-75
score: 230.603
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 95..360
e-value: 2.3E-87
score: 294.3
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 102..344
IPR034294Aquaporin transporterPANTHERPTHR45687AQUAPORIN OR AQUAGLYCEROPORIN RELATEDcoord: 78..357
NoneNo IPR availablePANTHERPTHR45687:SF66AQUAPORIN PIP2-1coord: 78..357
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 178..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G027000.1CmoCh04G027000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity