CmoCh04G026670 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G026670
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectate lyase
LocationCmo_Chr04: 19398147 .. 19401954 (+)
RNA-Seq ExpressionCmoCh04G026670
SyntenyCmoCh04G026670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAAAGTAAAGAATAACATGAAAGCGGAGGAACATTAAACAGACTGTCTCTCTCACCGGAGCAGAGGATTTTTAATGCACAGACAGAAACAGAACCAGAAGAAAGAAAAAGAAAGAACAAAGGAAGAAGGAAGAATGGGTTCAACAATTGGAGAAGAAAGCCCACGTTCCCCCCTTGTCTCTCTGGATTATACCCTTTTAGAATAATCTTTTCAAAGAAAGAGAATCTAAAGTGTTCTTTCGCCATTTCCCATTGTTCCCAAGTTGTTTTGTTAGCAGATTCATGTATAGTTTAGTTATTAAGCAGTGTTCTTGTTCTTGTTCATGTTCATTTCAAGAAGAAGGTACCTGAAATAGAGTTCTTCTTGTTCTTGTTCTGCTTCTTCATTTCAACAAAATCTACAAGCAGACATGTTTGCTGCCATTGCCATTCTGTTGTTCAGCTGGTTTTTGAGCTCCTCTCTGCCTTCTATGGCAGCTTTCAGTTTCAATCTCACTGACCCACATCTTCTTCCAGATCCTGAAACTGTTGCTTATGAAGTTCAGAGGTAAACAAGCAAGTTTCCACTGATTCAGGCTTTGTCCAGAACATGCCTCTAATGGGGTTCTGCAGTTGTTCTTGTTTAGGCATTTTAACAAACACCTTGTGTGCATTGTTGTTCTGCTCAAAGGTGCAGTCCCCTGCTTCAATGGCTTCCTTCCCACTAATTTGGTGATATCTATGTTTCTTGCAGGAGTGTGAATGATTCATTTTCAAGAAGGGAAATGCTGTCGATTCATTCTAAAGGTCAATCGAATTCCTGTCAAACTGGAAACCCTATCGATGATTGTTGGCGCTGCGGTTCGAACTGGCAAGACAATCGCCAACGGCTTGCAGATTGCAGCATTGGATTTGGCCGAAACGCCATGGGAGGTAAAGGAGGTCAGATCTACATAGTTACAGATTCCTCCGACTCCGACCCTGTAAACCCTCGCCCTGGAACTCTCCGCTACGCTGTGGTTCAAGATGAACCTCTCTGGATCGTCTTTGCTGCAGACATGACTATCAAACTCAAGTACGAACTCATGATGAACAGTTACAAAACCCTAGATGGCCGCGGCGCAAATGTCCACATAACCGGCGGCGGTTGCATCACACTTCAGTACATCTCGAATGTAATCATCCACAACATCAACATTCATCACTGTATCCCTACCGGTCACACTAACGTCCGATCAAGCCCTACGCACGTCGGATACAGAGGAAAGTCCGACGGCGATGGAATCTCAATCTTCAGCTCGCGCAACATCTGGATCGATCATTGCTCCTTATCGTACTGTACCGACGGCTTAATCGACGCCATAATGGGATCGACCGGAATCACGATCTCGAACAACTACTTCTCGCACCACGACGAGGTGATGCTGCTGGGGCATGAGGACGGCTATACGCCAGACTCGGGGATGCAGGTGACGATTGCGTTTAATTTGTTCGGCGAGAAGCTGGTTCAGCGGATGCCGCGGTGCCGGCGGGGTTATATACACGTGGTCAACAACGAGTTCCGATCGTGGGAGATGTACGCCATCGGCGGTAGCGGTGGTCCGACGATCAACAGCCAAGGGAATCGGTATATTGCGCCGGGGAATCCCAATGCGAAGGAGGTATGTTAACGTTATTACTCTTTTCTTCTCTCCACTCGTTGTACAGTCCACGGCGCGTATTCTGAACGCGCCGCTGAGGGAAATTATTTATAGGGAAGTTTTCGGGGCTTTAAGGTAATATCACTAATTCCATTTGGTTTTTAGTTTTTTTTCTAACTTTTTCAAAATTTTAATTTTAGCAATTTCTGATAACGTAGGGGCAAAACTGAAAATTTTAAAATATACAAGGACCAAATGTAAAATATGTAAAGTCAATGATTTGACGGGACAGTCCTCAGCCACTGGCTTCCTGTTTTGGACGACCTGTGTATAGTCAAAGAAGTGAAACGGTGTCGTTTTAATGCAGGTGACGAAGCGCGTGGACACAAACGCGGGGGATTGGTCGGAGTGGAACTGGAGGACGGAGGGGGACATTATGGTAAATGGTGCATTTTTTGTGCCGTCCGGCGAAGGACTGAGCAATATGTACGTTAAAGCTTCGAGTGTGGCGCCCAAATCCGCCGCTCTTGTCGACCAACTCACTCTCAACGCCGGCGTCTTCGGCGGGACCAGGTACTTTTTCTTTCTAAATGACCAAATTGCCCTTCATTTTTTCTTTTTTTTTTTCTTTTTGATTAAAATTCATACTCTCTATAGTTGAAAATTTCAATTGGGTCGTGATAGTTGAGGCCTAGTTTTTCATTCGAGTTTAGAGTTTCAATATGAAATGACCAAAATACCCTTATTTTTTTAACCTCAAGATTTGGCGTAGTTAATGGAGAGACAATTTATGCTCGGAAAGTAGCTTTGATCTATCTCGAGGATAAATACGTTCGAGATTCTAGAGATCGGGGAGTAAATGTTAAAAACGTGGTCCTTATAATTTTAAATTTTAGAATTTTATAATTGGATATAACCTCATAAATAACTTATCTAGACCTTTATTATAAAAAAAAAAAAAAAACAAAAACAAATAATTTTAACTAAATTACAAATTTATGGATAAATTGAATGGTTAAATGAGATATAGTATGTGGGACTTTGGAGTGAGAATCTAGGGCTGACACAACAGTCGAAAAACACAGCTCACTTGTTTTCTTGTTCTTGTCTTTTGTCTGTTCTGTCCACCTCTCCATTGGGCAAGCAAGCAATACCTTTTGCTTTTCCAAACACACCAAAATCTCCTTCAAGTAGGATTGAGAGAGATTGGGTTAAATTTTCTGGTTAAAATAATTGTTACCAATATGCTAGATTACAGTATAAACAAGTGTTTAGTAAATGTAGCGATCTCGATATATGATCGTTTATTAATTAATCGAGATACTTACAACGTTCAACTCTATTCGGTAATATGAGCTCGATTAGAAGGGTAAAGTTGTCCCCTGTGGATTAGAAAAGAAAGGAAAAAGTTAGCCTTTTAAGTTGTAAGCATAAATAAAGGGTAAAAGGGAAGAGGGTTATAGTGCATGTGTGGGGAAAAGAAGCATTAGCAGTTGGGGCAAGTGGGGGTTCTTCCATTCATACCATAATCTGGGGTTTGCCCATTTCTCTTTAAGAGTTGGCCAATTAGGCTTTATTCTATCTAACTCAAACTTCACCAACCAAATCATAGAGCTAGGAATTGAGTGATTATTTGATGTTCATCTTGATGGATACTCAAGCATTGTTCCATTTTGAATGATGTAGGGAGAGTAGACAGAGCAGCTCGTACCCTGGAGACGAGAGCTTTTCCAATTCCGGGTCCAGTGGCAGCGATGGCGGTGGTGACTACTTCGGGATGATATTCGGCGGGGGCGGTGTGAACAGTGCACTGCCACCATCTCTTTCAGTTGTTAACATGGTCGTTCTAACTCTGCTTACTATTTTAGTTCTGTACACAAACTCCATCTATGATGCTTTATTATCATTGACAGCATTGTAGTACTTGGAAAAGTTCAGAAAATGTTATATAAACTACAAAAGGAAAAGAAAAAAAAGATGCCTCTTTCTATATCTTATATGTACGTGTTTACTATAATTAAAATAAGGCATGGTATGGCAAAGAAGCAAAAAGGAGGTGAATTTCAAACGTACCAGTAAAACATCAACGTTGTCGAAACATGGGTTGTCTGTCTGTCGGTGGAAATATTTGACAAAATGGAATCTTTGGAACCGCAGCATGTGGAAATGTGGATGATGTCTCCCTCCC

mRNA sequence

ATAAAAGTAAAGAATAACATGAAAGCGGAGGAACATTAAACAGACTGTCTCTCTCACCGGAGCAGAGGATTTTTAATGCACAGACAGAAACAGAACCAGAAGAAAGAAAAAGAAAGAACAAAGGAAGAAGGAAGAATGGGTTCAACAATTGGAGAAGAAAGCCCACGTTCCCCCCTTGTCTCTCTGGATTATACCCTTTTAGAATAATCTTTTCAAAGAAAGAGAATCTAAAGTGTTCTTTCGCCATTTCCCATTGTTCCCAAGTTGTTTTGTTAGCAGATTCATGTATAGTTTAGTTATTAAGCAGTGTTCTTGTTCTTGTTCATGTTCATTTCAAGAAGAAGGTACCTGAAATAGAGTTCTTCTTGTTCTTGTTCTGCTTCTTCATTTCAACAAAATCTACAAGCAGACATGTTTGCTGCCATTGCCATTCTGTTGTTCAGCTGGTTTTTGAGCTCCTCTCTGCCTTCTATGGCAGCTTTCAGTTTCAATCTCACTGACCCACATCTTCTTCCAGATCCTGAAACTGTTGCTTATGAAGTTCAGAGGCTTTGTCCAGAACATGCCTCTAATGGGGTTCTGCAGTTGTTCTTGTTTAGGCATTTTAACAAACACCTTGTGTGCATTGTTGTTCTGCTCAAAGGTGCAGTCCCCTGCTTCAATGGCTTCCTTCCCACTAATTTGGTGATATCTATGAGTGTGAATGATTCATTTTCAAGAAGGGAAATGCTGTCGATTCATTCTAAAGGTCAATCGAATTCCTGTCAAACTGGAAACCCTATCGATGATTGTTGGCGCTGCGGTTCGAACTGGCAAGACAATCGCCAACGGCTTGCAGATTGCAGCATTGGATTTGGCCGAAACGCCATGGGAGGTAAAGGAGGTCAGATCTACATAGTTACAGATTCCTCCGACTCCGACCCTGTAAACCCTCGCCCTGGAACTCTCCGCTACGCTGTGGTTCAAGATGAACCTCTCTGGATCGTCTTTGCTGCAGACATGACTATCAAACTCAAGTACGAACTCATGATGAACAGTTACAAAACCCTAGATGGCCGCGGCGCAAATGTCCACATAACCGGCGGCGGTTGCATCACACTTCAGTACATCTCGAATGTAATCATCCACAACATCAACATTCATCACTGTATCCCTACCGGTCACACTAACGTCCGATCAAGCCCTACGCACGTCGGATACAGAGGAAAGTCCGACGGCGATGGAATCTCAATCTTCAGCTCGCGCAACATCTGGATCGATCATTGCTCCTTATCGTACTGTACCGACGGCTTAATCGACGCCATAATGGGATCGACCGGAATCACGATCTCGAACAACTACTTCTCGCACCACGACGAGGTGATGCTGCTGGGGCATGAGGACGGCTATACGCCAGACTCGGGGATGCAGGTGACGATTGCGTTTAATTTGTTCGGCGAGAAGCTGGTTCAGCGGATGCCGCGGTGCCGGCGGGGTTATATACACGTGGTCAACAACGAGTTCCGATCGTGGGAGATGTACGCCATCGGCGGTAGCGGTGGTCCGACGATCAACAGCCAAGGGAATCGGTATATTGCGCCGGGGAATCCCAATGCGAAGGAGTCCACGGCGCGTATTCTGAACGCGCCGCTGAGGGAAATTATTTATAGGGAAGTTTTCGGGGCTTTAAGTTTTTTTTCTAACTTTTTCAAAATTTTAATTTTAGCAATTTCTGATAACGTAGGGGCAAAACTGAAAATTTTAAAATATACAAGGACCAAATGTAAAATATGTAAAGTCAATGATTTGACGGGACAGTCCTCAGCCACTGGCTTCCTGTTTTGGACGACCTGTGTGACGAAGCGCGTGGACACAAACGCGGGGGATTGGTCGGAGTGGAACTGGAGGACGGAGGGGGACATTATGGTAAATGGTGCATTTTTTGTGCCGTCCGGCGAAGGACTGAGCAATATGTACGTTAAAGCTTCGAGTGTGGCGCCCAAATCCGCCGCTCTTGTCGACCAACTCACTCTCAACGCCGGCGTCTTCGGCGGGACCAGGGAGAGTAGACAGAGCAGCTCGTACCCTGGAGACGAGAGCTTTTCCAATTCCGGGTCCAGTGGCAGCGATGGCGGTGGTGACTACTTCGGGATGATATTCGGCGGGGGCGGTGTGAACAGTGCACTGCCACCATCTCTTTCAGTTGTTAACATGGTCGTTCTAACTCTGCTTACTATTTTAGTTCTGTACACAAACTCCATCTATGATGCTTTATTATCATTGACAGCATTGTAGTACTTGGAAAAGTTCAGAAAATGTTATATAAACTACAAAAGGAAAAGAAAAAAAAGATGCCTCTTTCTATATCTTATATGTACGTGTTTACTATAATTAAAATAAGGCATGGTATGGCAAAGAAGCAAAAAGGAGGTGAATTTCAAACGTACCAGTAAAACATCAACGTTGTCGAAACATGGGTTGTCTGTCTGTCGGTGGAAATATTTGACAAAATGGAATCTTTGGAACCGCAGCATGTGGAAATGTGGATGATGTCTCCCTCCC

Coding sequence (CDS)

ATGTTTGCTGCCATTGCCATTCTGTTGTTCAGCTGGTTTTTGAGCTCCTCTCTGCCTTCTATGGCAGCTTTCAGTTTCAATCTCACTGACCCACATCTTCTTCCAGATCCTGAAACTGTTGCTTATGAAGTTCAGAGGCTTTGTCCAGAACATGCCTCTAATGGGGTTCTGCAGTTGTTCTTGTTTAGGCATTTTAACAAACACCTTGTGTGCATTGTTGTTCTGCTCAAAGGTGCAGTCCCCTGCTTCAATGGCTTCCTTCCCACTAATTTGGTGATATCTATGAGTGTGAATGATTCATTTTCAAGAAGGGAAATGCTGTCGATTCATTCTAAAGGTCAATCGAATTCCTGTCAAACTGGAAACCCTATCGATGATTGTTGGCGCTGCGGTTCGAACTGGCAAGACAATCGCCAACGGCTTGCAGATTGCAGCATTGGATTTGGCCGAAACGCCATGGGAGGTAAAGGAGGTCAGATCTACATAGTTACAGATTCCTCCGACTCCGACCCTGTAAACCCTCGCCCTGGAACTCTCCGCTACGCTGTGGTTCAAGATGAACCTCTCTGGATCGTCTTTGCTGCAGACATGACTATCAAACTCAAGTACGAACTCATGATGAACAGTTACAAAACCCTAGATGGCCGCGGCGCAAATGTCCACATAACCGGCGGCGGTTGCATCACACTTCAGTACATCTCGAATGTAATCATCCACAACATCAACATTCATCACTGTATCCCTACCGGTCACACTAACGTCCGATCAAGCCCTACGCACGTCGGATACAGAGGAAAGTCCGACGGCGATGGAATCTCAATCTTCAGCTCGCGCAACATCTGGATCGATCATTGCTCCTTATCGTACTGTACCGACGGCTTAATCGACGCCATAATGGGATCGACCGGAATCACGATCTCGAACAACTACTTCTCGCACCACGACGAGGTGATGCTGCTGGGGCATGAGGACGGCTATACGCCAGACTCGGGGATGCAGGTGACGATTGCGTTTAATTTGTTCGGCGAGAAGCTGGTTCAGCGGATGCCGCGGTGCCGGCGGGGTTATATACACGTGGTCAACAACGAGTTCCGATCGTGGGAGATGTACGCCATCGGCGGTAGCGGTGGTCCGACGATCAACAGCCAAGGGAATCGGTATATTGCGCCGGGGAATCCCAATGCGAAGGAGTCCACGGCGCGTATTCTGAACGCGCCGCTGAGGGAAATTATTTATAGGGAAGTTTTCGGGGCTTTAAGTTTTTTTTCTAACTTTTTCAAAATTTTAATTTTAGCAATTTCTGATAACGTAGGGGCAAAACTGAAAATTTTAAAATATACAAGGACCAAATGTAAAATATGTAAAGTCAATGATTTGACGGGACAGTCCTCAGCCACTGGCTTCCTGTTTTGGACGACCTGTGTGACGAAGCGCGTGGACACAAACGCGGGGGATTGGTCGGAGTGGAACTGGAGGACGGAGGGGGACATTATGGTAAATGGTGCATTTTTTGTGCCGTCCGGCGAAGGACTGAGCAATATGTACGTTAAAGCTTCGAGTGTGGCGCCCAAATCCGCCGCTCTTGTCGACCAACTCACTCTCAACGCCGGCGTCTTCGGCGGGACCAGGGAGAGTAGACAGAGCAGCTCGTACCCTGGAGACGAGAGCTTTTCCAATTCCGGGTCCAGTGGCAGCGATGGCGGTGGTGACTACTTCGGGATGATATTCGGCGGGGGCGGTGTGAACAGTGCACTGCCACCATCTCTTTCAGTTGTTAACATGGTCGTTCTAACTCTGCTTACTATTTTAGTTCTGTACACAAACTCCATCTATGATGCTTTATTATCATTGACAGCATTGTAG

Protein sequence

MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQLFLFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGALSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKRVDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLLTILVLYTNSIYDALLSLTAL
Homology
BLAST of CmoCh04G026670 vs. ExPASy Swiss-Prot
Match: Q9SCP2 (Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2)

HSP 1 Score: 550.8 bits (1418), Expect = 1.9e-155
Identity = 289/517 (55.90%), Postives = 339/517 (65.57%), Query Frame = 0

Query: 93  ISMSVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNA 152
           +   VN S +RR+ L    +  S  C TGNPIDDCW+C  NW +NRQ LADC IGFG+ A
Sbjct: 45  VQWKVNASITRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYA 104

Query: 153 MGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKT 212
           +GGKGGQ Y VTDSSD D VNP+PGTLRY V+Q+EPLWIVF ++M IKLK EL+ NSYKT
Sbjct: 105 LGGKGGQFYFVTDSSDDDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKT 164

Query: 213 LDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGI 272
           LDGRGANVHI GGGCITLQY+SN+IIHNI+IHHC  +G+TNVRSSPTH G+R KSDGDGI
Sbjct: 165 LDGRGANVHIVGGGCITLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGI 224

Query: 273 SIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGM 332
           SIF S++IWIDHCSLS C DGLIDA+MGSTGITISNN+FSHH+EVMLLGH D Y PDSGM
Sbjct: 225 SIFGSKDIWIDHCSLSRCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGM 284

Query: 333 QVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGN 392
           QVTIAFN FGEKL+QRMPRCRRGYIHVVNN+F  WEMYAIGGSG PTINSQGNRY AP N
Sbjct: 285 QVTIAFNHFGEKLIQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTN 344

Query: 393 PNAKESTARILNAPLREIIYREVFGALSFFSNFFKILILAISDNVGAKLKILKYTRTKCK 452
           P AKE                                                       
Sbjct: 345 PFAKE------------------------------------------------------- 404

Query: 453 ICKVNDLTGQSSATGFLFWTTCVTKRVDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLS 512
                                 VTKRV+T  GDW  WNWR+EGDI+VNGAFFV SGEG  
Sbjct: 405 ----------------------VTKRVETPDGDWKGWNWRSEGDILVNGAFFVASGEGAE 464

Query: 513 NMYVKASSVAPKSAALVDQLTLNAGVFGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYF 572
             Y KA SV PKSA+ + Q+T ++GV G    +     +    +  + G+SG D   DY 
Sbjct: 465 MRYEKAYSVEPKSASFITQITFHSGVLGVGGRNNNLGMW---TTTGSEGTSGLDSYNDYT 477

Query: 573 GMIFGGGGVNSALPPSLSVVNMVVLTLLTILVLYTNS 610
             + G G  N     S SV+ + +L+ ++ LV++T+S
Sbjct: 525 DEMSGAGSTNRL---SFSVL-VFLLSSISYLVVFTSS 477

BLAST of CmoCh04G026670 vs. ExPASy Swiss-Prot
Match: Q93Z04 (Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 4.3e-147
Identity = 283/546 (51.83%), Postives = 345/546 (63.19%), Query Frame = 0

Query: 69  LVCIVVLLKGAVPCFNGFLP--------TNLVISMSVNDSFSRREM------LSIHSKGQ 128
           L+C+   L  A    N  LP          L +  SVN+S +RR++       S  S   
Sbjct: 12  LLCLFFTLLSATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSS 71

Query: 129 SNSCQTGNPIDDCWRCG-SNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVN 188
           S+SC+TGNPIDDCWRC  ++W  NRQRLADCSIGFG   +GGK G+IY+VTDSSD++P N
Sbjct: 72  SSSCRTGNPIDDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTN 131

Query: 189 PRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYI 248
           P PGTLRY V+Q+EPLWIVF+++M I+LK EL++NSYKTLDGRG+ VHITG GC+TLQY+
Sbjct: 132 PTPGTLRYGVIQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYV 191

Query: 249 SNVIIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDG 308
            ++IIHN++I+ C P+              RG+SDGDGISIF S+ IW+DHCS+S+CTDG
Sbjct: 192 QHIIIHNLHIYDCKPSAGFE---------KRGRSDGDGISIFGSQKIWVDHCSMSHCTDG 251

Query: 309 LIDAIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCR 368
           LIDA+MGST ITISNNYF+HHDEVMLLGH+D Y PD+GMQVTIAFN FG+ LVQRMPRCR
Sbjct: 252 LIDAVMGSTAITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCR 311

Query: 369 RGYIHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYR 428
           RGYIHVVNN+F  W+MYAIGGSG PTINSQGNRY AP +P+AKE                
Sbjct: 312 RGYIHVVNNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKE---------------- 371

Query: 429 EVFGALSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTT 488
                                                                       
Sbjct: 372 ------------------------------------------------------------ 431

Query: 489 CVTKRVDT-NAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQL 548
            VTKRVD+ + G+WS WNWRTEGD+M NGAFFV SGEG+S+MY KASSV PK+A+LVDQL
Sbjct: 432 -VTKRVDSKDDGEWSNWNWRTEGDLMENGAFFVASGEGMSSMYSKASSVDPKAASLVDQL 471

Query: 549 TLNAGVFGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGG---GVNSALPPSL 596
           T NAGVFGG R+ +  S         + G  GS GG    GM   GG   G +S+     
Sbjct: 492 TRNAGVFGGPRDDQGQSGNSYSPYGGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDS 471

BLAST of CmoCh04G026670 vs. ExPASy Swiss-Prot
Match: Q9SVQ6 (Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2)

HSP 1 Score: 478.0 bits (1229), Expect = 1.6e-133
Identity = 238/446 (53.36%), Postives = 287/446 (64.35%), Query Frame = 0

Query: 92  VISMSVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRN 151
           ++ MS+ +S  RR++      G  +SC TGNPIDDCWRC   W   R+RLADC+IGFGRN
Sbjct: 66  MVDMSIRNSTYRRKL------GFFSSCSTGNPIDDCWRCDKKWHRRRKRLADCAIGFGRN 125

Query: 152 AMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYK 211
           A+GG+ G+ YIVTD SD DPV P+PGTLRYAV+QDEPLWIVF  DM I L  EL+MNS+K
Sbjct: 126 AVGGRDGRYYIVTDPSDHDPVTPKPGTLRYAVIQDEPLWIVFKRDMVITLSQELIMNSFK 185

Query: 212 TLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDG 271
           T+DGRG NVHI GG C+T+QY++N+IIH INIH C  TG+  VRSS +H G+R  +DGDG
Sbjct: 186 TIDGRGVNVHIAGGACLTVQYVTNIIIHGINIHDCKRTGNAMVRSSESHYGWRTMADGDG 245

Query: 272 ISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSG 331
           ISIF S +IWIDH SLS C DGLIDAIMGST ITISNNY +HH+E +LLGH D YT D  
Sbjct: 246 ISIFGSSHIWIDHNSLSSCADGLIDAIMGSTAITISNNYLTHHNEAILLGHTDSYTRDKM 305

Query: 332 MQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPG 391
           MQVTIA+N FGE L+QRMPRCR GY HVVNN++  WEMYAIGGS  PTINSQGNR++APG
Sbjct: 306 MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPG 365

Query: 392 NPNAKESTARILNAPLREIIYREVFGALSFFSNFFKILILAISDNVGAKLKILKYTRTKC 451
           N  AKE                                                      
Sbjct: 366 NRFAKE------------------------------------------------------ 425

Query: 452 KICKVNDLTGQSSATGFLFWTTCVTKRVDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGL 511
                                  VTKRV    G+W+ WNWR++GD+M+NGA+F  SG G 
Sbjct: 426 -----------------------VTKRVGAGKGEWNNWNWRSQGDLMLNGAYFTSSGAGA 428

Query: 512 SNMYVKASSVAPKSAALVDQLTLNAG 538
           S  Y +ASS+A KS++LV  LT ++G
Sbjct: 486 SANYARASSLAAKSSSLVGMLTSSSG 428

BLAST of CmoCh04G026670 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 2.1e-133
Identity = 232/424 (54.72%), Postives = 282/424 (66.51%), Query Frame = 0

Query: 117 SCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRP 176
           SC TGNPIDDCWRC  NW  NR+RLADC IGFGRNA+GG+ G+ YIVTD +D D VNP+P
Sbjct: 117 SCGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKP 176

Query: 177 GTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNV 236
           GTLR+AV+Q+EPLWIVF  DM I+LK EL+MNS+KT+D RG+NVHI  G CIT+Q+I+NV
Sbjct: 177 GTLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNV 236

Query: 237 IIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLID 296
           IIH ++IH C PTG+  VRSSP+H G+R  +DGD +SIF S +IWIDH SLS+C DGL+D
Sbjct: 237 IIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVD 296

Query: 297 AIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGY 356
           A+MGST IT+SNN+F+HH+EVMLLGH D YT D  MQVTIA+N FGE LVQRMPRCR GY
Sbjct: 297 AVMGSTAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGY 356

Query: 357 IHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVF 416
            HVVNN++  WEMYAIGGS  PTINSQGNRY AP +  AKE                   
Sbjct: 357 FHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKE------------------- 416

Query: 417 GALSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVT 476
                                                                     VT
Sbjct: 417 ----------------------------------------------------------VT 463

Query: 477 KRVDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNA 536
           KRV+T+A +W +WNWR+EGD+++NGAFF PSG G S  Y +ASS+A K +++VD +T  A
Sbjct: 477 KRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTA 463

Query: 537 GVFG 541
           G  G
Sbjct: 537 GALG 463

BLAST of CmoCh04G026670 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 472.2 bits (1214), Expect = 8.8e-132
Identity = 232/434 (53.46%), Postives = 279/434 (64.29%), Query Frame = 0

Query: 117 SCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRP 176
           +C TGNPIDDCWRC  NW  NR+RLADC IGFGRNA+GG+ G+ Y+VTD  D +PVNPRP
Sbjct: 76  TCGTGNPIDDCWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRP 135

Query: 177 GTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNV 236
           GTLR+AV+QD PLWIVF  DM I+LK EL++NS+KT+DGRGANVHI  GGCIT+Q+++NV
Sbjct: 136 GTLRHAVIQDRPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNV 195

Query: 237 IIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLID 296
           I+H ++IH C PTG+  VRSS TH G+R  +DGD ISIF S ++WIDH SLS+C DGL+D
Sbjct: 196 IVHGLHIHDCKPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVD 255

Query: 297 AIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGY 356
           A+MGST ITISNN+ +HH+EVMLLGH D Y  D  MQVTIA+N FG  L+QRMPRCR GY
Sbjct: 256 AVMGSTAITISNNHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGY 315

Query: 357 IHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVF 416
            HVVNN++  WEMYAIGGS  PTINSQGNRY AP NP AKE                   
Sbjct: 316 FHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPKNPFAKE------------------- 375

Query: 417 GALSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVT 476
                                                                     VT
Sbjct: 376 ----------------------------------------------------------VT 431

Query: 477 KRVDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNA 536
           KRVDT A  W  WNWR+EGD++ NGA+F  SG   S  Y +ASS++ KS++LV  +T +A
Sbjct: 436 KRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGSYARASSLSAKSSSLVGHITSDA 431

Query: 537 GVFGGTRESRQSSS 551
           G     R  RQ SS
Sbjct: 496 GAL-PCRRGRQCSS 431

BLAST of CmoCh04G026670 vs. ExPASy TrEMBL
Match: A0A6J1H1R2 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111459254 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 3.6e-269
Identity = 494/620 (79.68%), Postives = 494/620 (79.68%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQLF 60
           MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQR              
Sbjct: 1   MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQR-------------- 60

Query: 61  LFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSCQT 120
                                              SVNDSFSRREMLSIHSKGQSNSCQT
Sbjct: 61  -----------------------------------SVNDSFSRREMLSIHSKGQSNSCQT 120

Query: 121 GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR 180
           GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR 180

Query: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240
           YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN
Sbjct: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240

Query: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300
           INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG
Sbjct: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300

Query: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360
           STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV
Sbjct: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360

Query: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGALS 420
           NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE                       
Sbjct: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE----------------------- 420

Query: 421 FFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKRVD 480
                                                                 VTKRVD
Sbjct: 421 ------------------------------------------------------VTKRVD 480

Query: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 540
           TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG
Sbjct: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 494

Query: 541 GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 600
           GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL
Sbjct: 541 GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 494

Query: 601 TILVLYTNSIYDALLSLTAL 621
           TILVLYTNSIYDALLSLTAL
Sbjct: 601 TILVLYTNSIYDALLSLTAL 494

BLAST of CmoCh04G026670 vs. ExPASy TrEMBL
Match: A0A6J1JIE0 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111487228 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.4e-265
Identity = 488/620 (78.71%), Postives = 491/620 (79.19%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQLF 60
           M AAIAILLFSWFL+SSLPSMAAFSFNLTDPHLLPDPETVAYEVQR              
Sbjct: 1   MLAAIAILLFSWFLNSSLPSMAAFSFNLTDPHLLPDPETVAYEVQR-------------- 60

Query: 61  LFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSCQT 120
                                              SVNDSFSRREMLSIHSKGQSNSCQT
Sbjct: 61  -----------------------------------SVNDSFSRREMLSIHSKGQSNSCQT 120

Query: 121 GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR 180
           GNPIDDCWRCGSNWQ NRQRLADCSIGFGRNAMGGKGGQIYIVTDSSD+DPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCGSNWQANRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDTDPVNPRPGTLR 180

Query: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240
           YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN
Sbjct: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240

Query: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300
           INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG
Sbjct: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300

Query: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360
           STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV
Sbjct: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360

Query: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGALS 420
           NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE                       
Sbjct: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE----------------------- 420

Query: 421 FFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKRVD 480
                                                                 VTKRVD
Sbjct: 421 ------------------------------------------------------VTKRVD 480

Query: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 540
           TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG
Sbjct: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 494

Query: 541 GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 600
           GTRESRQSSSY GDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL
Sbjct: 541 GTRESRQSSSYSGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 494

Query: 601 TILVLYTNSIYDALLSLTAL 621
           TILVLYTNSIY+ALLSLTAL
Sbjct: 601 TILVLYTNSIYNALLSLTAL 494

BLAST of CmoCh04G026670 vs. ExPASy TrEMBL
Match: A0A5D3BPB8 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00090 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 9.1e-241
Identity = 441/622 (70.90%), Postives = 469/622 (75.40%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSL--PSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQ 60
           MFA   +L F WFLS +L   +MA F+FNLT  +LLPDPETVAY+VQR            
Sbjct: 1   MFATTGVLFFIWFLSCTLLPLTMATFNFNLTVQNLLPDPETVAYQVQR------------ 60

Query: 61  LFLFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSC 120
                                                SVNDSF RR+MLS+HSKGQS+SC
Sbjct: 61  -------------------------------------SVNDSFFRRQMLSVHSKGQSSSC 120

Query: 121 QTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGT 180
           QTGNPIDDCWRC SNWQ NRQRLADC IGFGR+AMGGKGGQIY+VTDSSD DPVNPRPGT
Sbjct: 121 QTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTDSSDPDPVNPRPGT 180

Query: 181 LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVII 240
           LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVII
Sbjct: 181 LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVII 240

Query: 241 HNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI 300
           HNINIHHC+PTGHTN+RSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI
Sbjct: 241 HNINIHHCVPTGHTNIRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI 300

Query: 301 MGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIH 360
           MGSTGITISNNYFSHHDEVMLLGH+DG+TPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIH
Sbjct: 301 MGSTGITISNNYFSHHDEVMLLGHDDGFTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIH 360

Query: 361 VVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGA 420
           VVNN+FRSWEMYAIGGSG PTINSQGNRYIAPGNPNAKE                     
Sbjct: 361 VVNNDFRSWEMYAIGGSGNPTINSQGNRYIAPGNPNAKE--------------------- 420

Query: 421 LSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKR 480
                                                                   VTKR
Sbjct: 421 --------------------------------------------------------VTKR 480

Query: 481 VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGV 540
           VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASS+ PKSAALVDQLTLNAGV
Sbjct: 481 VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSLPPKSAALVDQLTLNAGV 495

Query: 541 FGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLT 600
           FGG+RE+RQSSSYPGDE+ S+SGSSGSDGGGDYFGMIFGGGG+N+A PPSLS VNMV+LT
Sbjct: 541 FGGSRETRQSSSYPGDETSSSSGSSGSDGGGDYFGMIFGGGGINNAPPPSLSAVNMVILT 495

Query: 601 LLTILVLYTNSIYDALLSLTAL 621
           L T+L+LYTN+ YDALL   +L
Sbjct: 601 LFTVLILYTNN-YDALLLTLSL 495

BLAST of CmoCh04G026670 vs. ExPASy TrEMBL
Match: A0A1S3C4R4 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103496461 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 9.1e-241
Identity = 441/622 (70.90%), Postives = 469/622 (75.40%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSL--PSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQ 60
           MFA   +L F WFLS +L   +MA F+FNLT  +LLPDPETVAY+VQR            
Sbjct: 1   MFATTGVLFFIWFLSCTLLPLTMATFNFNLTVQNLLPDPETVAYQVQR------------ 60

Query: 61  LFLFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSC 120
                                                SVNDSF RR+MLS+HSKGQS+SC
Sbjct: 61  -------------------------------------SVNDSFFRRQMLSVHSKGQSSSC 120

Query: 121 QTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGT 180
           QTGNPIDDCWRC SNWQ NRQRLADC IGFGR+AMGGKGGQIY+VTDSSD DPVNPRPGT
Sbjct: 121 QTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTDSSDPDPVNPRPGT 180

Query: 181 LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVII 240
           LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVII
Sbjct: 181 LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVII 240

Query: 241 HNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI 300
           HNINIHHC+PTGHTN+RSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI
Sbjct: 241 HNINIHHCVPTGHTNIRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI 300

Query: 301 MGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIH 360
           MGSTGITISNNYFSHHDEVMLLGH+DG+TPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIH
Sbjct: 301 MGSTGITISNNYFSHHDEVMLLGHDDGFTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIH 360

Query: 361 VVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGA 420
           VVNN+FRSWEMYAIGGSG PTINSQGNRYIAPGNPNAKE                     
Sbjct: 361 VVNNDFRSWEMYAIGGSGNPTINSQGNRYIAPGNPNAKE--------------------- 420

Query: 421 LSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKR 480
                                                                   VTKR
Sbjct: 421 --------------------------------------------------------VTKR 480

Query: 481 VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGV 540
           VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASS+ PKSAALVDQLTLNAGV
Sbjct: 481 VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSLPPKSAALVDQLTLNAGV 495

Query: 541 FGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLT 600
           FGG+RE+RQSSSYPGDE+ S+SGSSGSDGGGDYFGMIFGGGG+N+A PPSLS VNMV+LT
Sbjct: 541 FGGSRETRQSSSYPGDETSSSSGSSGSDGGGDYFGMIFGGGGINNAPPPSLSAVNMVILT 495

Query: 601 LLTILVLYTNSIYDALLSLTAL 621
           L T+L+LYTN+ YDALL   +L
Sbjct: 601 LFTVLILYTNN-YDALLLTLSL 495

BLAST of CmoCh04G026670 vs. ExPASy TrEMBL
Match: A0A6J1FLS3 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111445375 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 1.5e-238
Identity = 444/621 (71.50%), Postives = 467/621 (75.20%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSL--PSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQ 60
           MFA   IL F W LSSSL  P+MA F+FNLT PHLLPDPETVAY+VQR            
Sbjct: 1   MFATTGILFFIWVLSSSLPPPAMATFAFNLTVPHLLPDPETVAYQVQR------------ 60

Query: 61  LFLFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSC 120
                                                +VNDSFSRR+MLS++SK QS+SC
Sbjct: 61  -------------------------------------AVNDSFSRRQMLSVNSKDQSSSC 120

Query: 121 QTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGT 180
           QTGNPIDDCWRC SNWQ NRQRLADC IGFGR+AMGGKGGQIY+VTDSSDSDPVNPRPGT
Sbjct: 121 QTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTDSSDSDPVNPRPGT 180

Query: 181 LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVII 240
           LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGC+TLQYISNVII
Sbjct: 181 LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCVTLQYISNVII 240

Query: 241 HNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI 300
           HNINIHHCIPTGHTN+RSSPTH+GYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI
Sbjct: 241 HNINIHHCIPTGHTNIRSSPTHIGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI 300

Query: 301 MGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIH 360
           MGSTGITISNNYFSHHDEVMLLGH+DGY+PDS MQVTIAFNLFGEKLVQRMPRCRRGY H
Sbjct: 301 MGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGYFH 360

Query: 361 VVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGA 420
           VVNN+FRSWEMYAIGGSG PTINSQGNRYIAPGNPNAKE                     
Sbjct: 361 VVNNDFRSWEMYAIGGSGDPTINSQGNRYIAPGNPNAKE--------------------- 420

Query: 421 LSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKR 480
                                                                   VTKR
Sbjct: 421 --------------------------------------------------------VTKR 480

Query: 481 VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGV 540
           VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVD+LTLNAGV
Sbjct: 481 VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDRLTLNAGV 495

Query: 541 FGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIF-GGGGVNSA-LPPSLSVVNMVV 600
           FGG RE+RQS SYPGDES S+S SSGSDGGGDYFGMIF GGGGVNSA  PP LSVVNM++
Sbjct: 541 FGGARENRQSYSYPGDESSSSSTSSGSDGGGDYFGMIFGGGGGVNSAPPPPCLSVVNMLM 495

Query: 601 LTLLTILVLYTNSIYDALLSL 618
           L+LLT+LVLYTNS   ALLSL
Sbjct: 601 LSLLTVLVLYTNSYDSALLSL 495

BLAST of CmoCh04G026670 vs. NCBI nr
Match: XP_022957835.1 (probable pectate lyase 12 [Cucurbita moschata] >KAG7032968.1 putative pectate lyase 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 937.2 bits (2421), Expect = 7.3e-269
Identity = 494/620 (79.68%), Postives = 494/620 (79.68%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQLF 60
           MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQR              
Sbjct: 1   MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQR-------------- 60

Query: 61  LFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSCQT 120
                                              SVNDSFSRREMLSIHSKGQSNSCQT
Sbjct: 61  -----------------------------------SVNDSFSRREMLSIHSKGQSNSCQT 120

Query: 121 GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR 180
           GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR 180

Query: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240
           YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN
Sbjct: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240

Query: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300
           INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG
Sbjct: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300

Query: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360
           STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV
Sbjct: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360

Query: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGALS 420
           NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE                       
Sbjct: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE----------------------- 420

Query: 421 FFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKRVD 480
                                                                 VTKRVD
Sbjct: 421 ------------------------------------------------------VTKRVD 480

Query: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 540
           TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG
Sbjct: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 494

Query: 541 GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 600
           GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL
Sbjct: 541 GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 494

Query: 601 TILVLYTNSIYDALLSLTAL 621
           TILVLYTNSIYDALLSLTAL
Sbjct: 601 TILVLYTNSIYDALLSLTAL 494

BLAST of CmoCh04G026670 vs. NCBI nr
Match: XP_023524526.1 (probable pectate lyase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 927.9 bits (2397), Expect = 4.5e-266
Identity = 489/620 (78.87%), Postives = 491/620 (79.19%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQLF 60
           MFAAIAILLF+WFLSSSLPS+AAFSFNLTDPHLLPDPETV YEVQR              
Sbjct: 1   MFAAIAILLFTWFLSSSLPSLAAFSFNLTDPHLLPDPETVVYEVQR-------------- 60

Query: 61  LFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSCQT 120
                                              SVNDSFSRREMLSIHSKGQSNSCQT
Sbjct: 61  -----------------------------------SVNDSFSRREMLSIHSKGQSNSCQT 120

Query: 121 GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR 180
           GNPIDDCWRCGSNWQ NRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCGSNWQANRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR 180

Query: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240
           YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN
Sbjct: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240

Query: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300
           INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG
Sbjct: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300

Query: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360
           STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV
Sbjct: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360

Query: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGALS 420
           NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE                       
Sbjct: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE----------------------- 420

Query: 421 FFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKRVD 480
                                                                 VTKRVD
Sbjct: 421 ------------------------------------------------------VTKRVD 480

Query: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 540
           TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG
Sbjct: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 494

Query: 541 GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 600
           GTRESRQSSSYPGDESFSNSGSSGSD GGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL
Sbjct: 541 GTRESRQSSSYPGDESFSNSGSSGSDSGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 494

Query: 601 TILVLYTNSIYDALLSLTAL 621
           TILVLYTNSIYDALLSLTAL
Sbjct: 601 TILVLYTNSIYDALLSLTAL 494

BLAST of CmoCh04G026670 vs. NCBI nr
Match: XP_022990332.1 (probable pectate lyase 12 [Cucurbita maxima])

HSP 1 Score: 924.5 bits (2388), Expect = 4.9e-265
Identity = 488/620 (78.71%), Postives = 491/620 (79.19%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSLPSMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQLF 60
           M AAIAILLFSWFL+SSLPSMAAFSFNLTDPHLLPDPETVAYEVQR              
Sbjct: 1   MLAAIAILLFSWFLNSSLPSMAAFSFNLTDPHLLPDPETVAYEVQR-------------- 60

Query: 61  LFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSCQT 120
                                              SVNDSFSRREMLSIHSKGQSNSCQT
Sbjct: 61  -----------------------------------SVNDSFSRREMLSIHSKGQSNSCQT 120

Query: 121 GNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLR 180
           GNPIDDCWRCGSNWQ NRQRLADCSIGFGRNAMGGKGGQIYIVTDSSD+DPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCGSNWQANRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDTDPVNPRPGTLR 180

Query: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240
           YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN
Sbjct: 181 YAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHN 240

Query: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300
           INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG
Sbjct: 241 INIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMG 300

Query: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360
           STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV
Sbjct: 301 STGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVV 360

Query: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGALS 420
           NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE                       
Sbjct: 361 NNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKE----------------------- 420

Query: 421 FFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKRVD 480
                                                                 VTKRVD
Sbjct: 421 ------------------------------------------------------VTKRVD 480

Query: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 540
           TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG
Sbjct: 481 TNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFG 494

Query: 541 GTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 600
           GTRESRQSSSY GDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL
Sbjct: 541 GTRESRQSSSYSGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLL 494

Query: 601 TILVLYTNSIYDALLSLTAL 621
           TILVLYTNSIY+ALLSLTAL
Sbjct: 601 TILVLYTNSIYNALLSLTAL 494

BLAST of CmoCh04G026670 vs. NCBI nr
Match: KAG6602287.1 (putative pectate lyase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 904.0 bits (2335), Expect = 6.9e-259
Identity = 474/600 (79.00%), Postives = 474/600 (79.00%), Query Frame = 0

Query: 21  MAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQLFLFRHFNKHLVCIVVLLKGAV 80
           MAAFSFNLTDPHLLPDPETVAYEVQR                                  
Sbjct: 1   MAAFSFNLTDPHLLPDPETVAYEVQR---------------------------------- 60

Query: 81  PCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQR 140
                          SVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQR
Sbjct: 61  ---------------SVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQR 120

Query: 141 LADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIK 200
           LADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIK
Sbjct: 121 LADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIK 180

Query: 201 LKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTH 260
           LKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTH
Sbjct: 181 LKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTH 240

Query: 261 VGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLL 320
           VGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLL
Sbjct: 241 VGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLL 300

Query: 321 GHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTI 380
           GHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTI
Sbjct: 301 GHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTI 360

Query: 381 NSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGALSFFSNFFKILILAISDNVGAK 440
           NSQGNRYIAPGNPNAKE                                           
Sbjct: 361 NSQGNRYIAPGNPNAKE------------------------------------------- 420

Query: 441 LKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKRVDTNAGDWSEWNWRTEGDIMVN 500
                                             VTKRVDTNAGDWSEWNWRTEGDIMVN
Sbjct: 421 ----------------------------------VTKRVDTNAGDWSEWNWRTEGDIMVN 474

Query: 501 GAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFGGTRESRQSSSYPGDESFSNS 560
           GAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFGGTRESRQSSSYPGDESFSNS
Sbjct: 481 GAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFGGTRESRQSSSYPGDESFSNS 474

Query: 561 GSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLLTILVLYTNSIYDALLSLTAL 620
           GSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLLTILVLYTNSIYDALLSLTAL
Sbjct: 541 GSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLLTILVLYTNSIYDALLSLTAL 474

BLAST of CmoCh04G026670 vs. NCBI nr
Match: XP_038884299.1 (probable pectate lyase 12 [Benincasa hispida])

HSP 1 Score: 863.2 bits (2229), Expect = 1.3e-246
Identity = 452/622 (72.67%), Postives = 473/622 (76.05%), Query Frame = 0

Query: 1   MFAAIAILLFSWFLSSSLP--SMAAFSFNLTDPHLLPDPETVAYEVQRLCPEHASNGVLQ 60
           MFA   IL F WFLSSSLP  ++A  +FNLT PHLLPDPETVAY+VQR            
Sbjct: 1   MFATTGILFFIWFLSSSLPPLTIANLNFNLTVPHLLPDPETVAYQVQR------------ 60

Query: 61  LFLFRHFNKHLVCIVVLLKGAVPCFNGFLPTNLVISMSVNDSFSRREMLSIHSKGQSNSC 120
                                                SVNDSFSRR+MLSIHSKGQSNSC
Sbjct: 61  -------------------------------------SVNDSFSRRQMLSIHSKGQSNSC 120

Query: 121 QTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGT 180
           QTGNPIDDCWRC SNWQ NRQRLADC IGFGR AMGGKGGQIYIVTDSSDSDPVNPRPGT
Sbjct: 121 QTGNPIDDCWRCDSNWQANRQRLADCGIGFGRAAMGGKGGQIYIVTDSSDSDPVNPRPGT 180

Query: 181 LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVII 240
           LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISN+II
Sbjct: 181 LRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNIII 240

Query: 241 HNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI 300
           HNINIHHC+PTGHTN+RSSPTH+GYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI
Sbjct: 241 HNINIHHCVPTGHTNIRSSPTHIGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAI 300

Query: 301 MGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIH 360
           MGSTGITISNNYFSHHDEVMLLGH+DG+TPDS MQVTIAFNLFGEKLVQRMPRCRRGYIH
Sbjct: 301 MGSTGITISNNYFSHHDEVMLLGHDDGFTPDSAMQVTIAFNLFGEKLVQRMPRCRRGYIH 360

Query: 361 VVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYREVFGA 420
           VVNN+FRSWEMYAIGGSG PTINSQGNRYIAPGNPNAKE                     
Sbjct: 361 VVNNDFRSWEMYAIGGSGNPTINSQGNRYIAPGNPNAKE--------------------- 420

Query: 421 LSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKR 480
                                                                   VTKR
Sbjct: 421 --------------------------------------------------------VTKR 480

Query: 481 VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGV 540
           VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASS+ PKSAALVDQLTLNAGV
Sbjct: 481 VDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSLPPKSAALVDQLTLNAGV 496

Query: 541 FGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLT 600
           FGG+RESRQS+SYPGDES ++SGSSGSDGGGDYFGMIFGGGG+NSA PPSLSVVN+V+LT
Sbjct: 541 FGGSRESRQSNSYPGDESTTSSGSSGSDGGGDYFGMIFGGGGMNSAPPPSLSVVNVVILT 496

Query: 601 LLTILVLYTNSIYDALLSLTAL 621
           L T+LVLYTNS YD LLSLT L
Sbjct: 601 LFTVLVLYTNSNYDVLLSLTTL 496

BLAST of CmoCh04G026670 vs. TAIR 10
Match: AT3G53190.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 550.8 bits (1418), Expect = 1.4e-156
Identity = 289/517 (55.90%), Postives = 339/517 (65.57%), Query Frame = 0

Query: 93  ISMSVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNA 152
           +   VN S +RR+ L    +  S  C TGNPIDDCW+C  NW +NRQ LADC IGFG+ A
Sbjct: 45  VQWKVNASITRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYA 104

Query: 153 MGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKT 212
           +GGKGGQ Y VTDSSD D VNP+PGTLRY V+Q+EPLWIVF ++M IKLK EL+ NSYKT
Sbjct: 105 LGGKGGQFYFVTDSSDDDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKT 164

Query: 213 LDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGI 272
           LDGRGANVHI GGGCITLQY+SN+IIHNI+IHHC  +G+TNVRSSPTH G+R KSDGDGI
Sbjct: 165 LDGRGANVHIVGGGCITLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGI 224

Query: 273 SIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGM 332
           SIF S++IWIDHCSLS C DGLIDA+MGSTGITISNN+FSHH+EVMLLGH D Y PDSGM
Sbjct: 225 SIFGSKDIWIDHCSLSRCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGM 284

Query: 333 QVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGN 392
           QVTIAFN FGEKL+QRMPRCRRGYIHVVNN+F  WEMYAIGGSG PTINSQGNRY AP N
Sbjct: 285 QVTIAFNHFGEKLIQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTN 344

Query: 393 PNAKESTARILNAPLREIIYREVFGALSFFSNFFKILILAISDNVGAKLKILKYTRTKCK 452
           P AKE                                                       
Sbjct: 345 PFAKE------------------------------------------------------- 404

Query: 453 ICKVNDLTGQSSATGFLFWTTCVTKRVDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGLS 512
                                 VTKRV+T  GDW  WNWR+EGDI+VNGAFFV SGEG  
Sbjct: 405 ----------------------VTKRVETPDGDWKGWNWRSEGDILVNGAFFVASGEGAE 464

Query: 513 NMYVKASSVAPKSAALVDQLTLNAGVFGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYF 572
             Y KA SV PKSA+ + Q+T ++GV G    +     +    +  + G+SG D   DY 
Sbjct: 465 MRYEKAYSVEPKSASFITQITFHSGVLGVGGRNNNLGMW---TTTGSEGTSGLDSYNDYT 477

Query: 573 GMIFGGGGVNSALPPSLSVVNMVVLTLLTILVLYTNS 610
             + G G  N     S SV+ + +L+ ++ LV++T+S
Sbjct: 525 DEMSGAGSTNRL---SFSVL-VFLLSSISYLVVFTSS 477

BLAST of CmoCh04G026670 vs. TAIR 10
Match: AT5G04310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 544.3 bits (1401), Expect = 1.3e-154
Identity = 286/535 (53.46%), Postives = 344/535 (64.30%), Query Frame = 0

Query: 91  LVISMSVNDSFSRREMLSIHSKGQSN------SCQTGNPIDDCWRCGSNWQDNRQRLADC 150
           L +   +NDS SRR++L+      +       SC TGNPIDDCWRC  NW  NRQRLADC
Sbjct: 58  LNVQRKLNDSLSRRQLLTYQQDDGTTASSPIPSCITGNPIDDCWRCDPNWSANRQRLADC 117

Query: 151 SIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYE 210
           SIGFG+  +GGKGGQ Y+VTDSSD+D  NP PGTLR+AV+Q EPLWI+F++DM IKLK+E
Sbjct: 118 SIGFGQGTLGGKGGQFYLVTDSSDNDAANPIPGTLRHAVIQPEPLWIIFSSDMGIKLKHE 177

Query: 211 LMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTHVGYR 270
           L++ SYKT+DGRG N+ ITG GC+T+Q +S+VIIHN++IHHC P+G+T V SSPTHVG+R
Sbjct: 178 LIIGSYKTIDGRGTNIQITGHGCLTIQQVSHVIIHNVHIHHCKPSGNTLVASSPTHVGFR 237

Query: 271 GKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHED 330
           G SDGDGIS+ +S +IW+DHCSL YC DGLID I+ ST +TISNNYFSHHDEVMLLGH+D
Sbjct: 238 GVSDGDGISVSASHHIWVDHCSLGYCADGLIDVILASTAVTISNNYFSHHDEVMLLGHDD 297

Query: 331 GYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTINSQG 390
            YT D GMQVTIAFN FGE LVQRMPRCR GYIHVVNN+F +WEMYAIGGS  PTINSQG
Sbjct: 298 RYTADKGMQVTIAFNHFGEGLVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSASPTINSQG 357

Query: 391 NRYIAPGNPNAKESTARILNAPLREIIYREVFGALSFFSNFFKILILAISDNVGAKLKIL 450
           NRY AP +PNAKE                                               
Sbjct: 358 NRYTAPIDPNAKE----------------------------------------------- 417

Query: 451 KYTRTKCKICKVNDLTGQSSATGFLFWTTCVTKRVDTNAGDWSEWNWRTEGDIMVNGAFF 510
                                         VTKRVD+N   WS WNWRTEGD+MVNGAFF
Sbjct: 418 ------------------------------VTKRVDSNEKHWSGWNWRTEGDVMVNGAFF 477

Query: 511 VPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFGG-TRESRQSSSYPG---------- 570
           VPSG+G+S  Y +A+SV PK+AA++DQLT+NAGVFG  +  + Q  S+PG          
Sbjct: 478 VPSGDGVSPAYARATSVQPKAAAIIDQLTVNAGVFGDPSGRNGQGGSFPGITNGGGTITR 509

Query: 571 --DESFSNSGSSGSDGGGDYFGMIFGGGGVNSALPPSLSVVNMVVLTLLTILVLY 607
              +S    G SGSD     F +IFG      AL P        V ++L I++LY
Sbjct: 538 GYSKSGPAGGGSGSDSDDGLFTLIFGNNSGAVALRPG------QVWSILLIIILY 509

BLAST of CmoCh04G026670 vs. TAIR 10
Match: AT3G54920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 523.1 bits (1346), Expect = 3.1e-148
Identity = 283/546 (51.83%), Postives = 345/546 (63.19%), Query Frame = 0

Query: 69  LVCIVVLLKGAVPCFNGFLP--------TNLVISMSVNDSFSRREM------LSIHSKGQ 128
           L+C+   L  A    N  LP          L +  SVN+S +RR++       S  S   
Sbjct: 12  LLCLFFTLLSATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSS 71

Query: 129 SNSCQTGNPIDDCWRCG-SNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVN 188
           S+SC+TGNPIDDCWRC  ++W  NRQRLADCSIGFG   +GGK G+IY+VTDSSD++P N
Sbjct: 72  SSSCRTGNPIDDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTN 131

Query: 189 PRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYI 248
           P PGTLRY V+Q+EPLWIVF+++M I+LK EL++NSYKTLDGRG+ VHITG GC+TLQY+
Sbjct: 132 PTPGTLRYGVIQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYV 191

Query: 249 SNVIIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDG 308
            ++IIHN++I+ C P+              RG+SDGDGISIF S+ IW+DHCS+S+CTDG
Sbjct: 192 QHIIIHNLHIYDCKPSAGFE---------KRGRSDGDGISIFGSQKIWVDHCSMSHCTDG 251

Query: 309 LIDAIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCR 368
           LIDA+MGST ITISNNYF+HHDEVMLLGH+D Y PD+GMQVTIAFN FG+ LVQRMPRCR
Sbjct: 252 LIDAVMGSTAITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCR 311

Query: 369 RGYIHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKESTARILNAPLREIIYR 428
           RGYIHVVNN+F  W+MYAIGGSG PTINSQGNRY AP +P+AKE                
Sbjct: 312 RGYIHVVNNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKE---------------- 371

Query: 429 EVFGALSFFSNFFKILILAISDNVGAKLKILKYTRTKCKICKVNDLTGQSSATGFLFWTT 488
                                                                       
Sbjct: 372 ------------------------------------------------------------ 431

Query: 489 CVTKRVDT-NAGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQL 548
            VTKRVD+ + G+WS WNWRTEGD+M NGAFFV SGEG+S+MY KASSV PK+A+LVDQL
Sbjct: 432 -VTKRVDSKDDGEWSNWNWRTEGDLMENGAFFVASGEGMSSMYSKASSVDPKAASLVDQL 471

Query: 549 TLNAGVFGGTRESRQSSSYPGDESFSNSGSSGSDGGGDYFGMIFGGG---GVNSALPPSL 596
           T NAGVFGG R+ +  S         + G  GS GG    GM   GG   G +S+     
Sbjct: 492 TRNAGVFGGPRDDQGQSGNSYSPYGGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDS 471

BLAST of CmoCh04G026670 vs. TAIR 10
Match: AT4G13210.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 478.0 bits (1229), Expect = 1.1e-134
Identity = 238/446 (53.36%), Postives = 287/446 (64.35%), Query Frame = 0

Query: 92  VISMSVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRN 151
           ++ MS+ +S  RR++      G  +SC TGNPIDDCWRC   W   R+RLADC+IGFGRN
Sbjct: 29  MVDMSIRNSTYRRKL------GFFSSCSTGNPIDDCWRCDKKWHRRRKRLADCAIGFGRN 88

Query: 152 AMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYK 211
           A+GG+ G+ YIVTD SD DPV P+PGTLRYAV+QDEPLWIVF  DM I L  EL+MNS+K
Sbjct: 89  AVGGRDGRYYIVTDPSDHDPVTPKPGTLRYAVIQDEPLWIVFKRDMVITLSQELIMNSFK 148

Query: 212 TLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDG 271
           T+DGRG NVHI GG C+T+QY++N+IIH INIH C  TG+  VRSS +H G+R  +DGDG
Sbjct: 149 TIDGRGVNVHIAGGACLTVQYVTNIIIHGINIHDCKRTGNAMVRSSESHYGWRTMADGDG 208

Query: 272 ISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSG 331
           ISIF S +IWIDH SLS C DGLIDAIMGST ITISNNY +HH+E +LLGH D YT D  
Sbjct: 209 ISIFGSSHIWIDHNSLSSCADGLIDAIMGSTAITISNNYLTHHNEAILLGHTDSYTRDKM 268

Query: 332 MQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPG 391
           MQVTIA+N FGE L+QRMPRCR GY HVVNN++  WEMYAIGGS  PTINSQGNR++APG
Sbjct: 269 MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPG 328

Query: 392 NPNAKESTARILNAPLREIIYREVFGALSFFSNFFKILILAISDNVGAKLKILKYTRTKC 451
           N  AKE                                                      
Sbjct: 329 NRFAKE------------------------------------------------------ 388

Query: 452 KICKVNDLTGQSSATGFLFWTTCVTKRVDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGL 511
                                  VTKRV    G+W+ WNWR++GD+M+NGA+F  SG G 
Sbjct: 389 -----------------------VTKRVGAGKGEWNNWNWRSQGDLMLNGAYFTSSGAGA 391

Query: 512 SNMYVKASSVAPKSAALVDQLTLNAG 538
           S  Y +ASS+A KS++LV  LT ++G
Sbjct: 449 SANYARASSLAAKSSSLVGMLTSSSG 391

BLAST of CmoCh04G026670 vs. TAIR 10
Match: AT4G13210.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 478.0 bits (1229), Expect = 1.1e-134
Identity = 238/446 (53.36%), Postives = 287/446 (64.35%), Query Frame = 0

Query: 92  VISMSVNDSFSRREMLSIHSKGQSNSCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRN 151
           ++ MS+ +S  RR++      G  +SC TGNPIDDCWRC   W   R+RLADC+IGFGRN
Sbjct: 66  MVDMSIRNSTYRRKL------GFFSSCSTGNPIDDCWRCDKKWHRRRKRLADCAIGFGRN 125

Query: 152 AMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYK 211
           A+GG+ G+ YIVTD SD DPV P+PGTLRYAV+QDEPLWIVF  DM I L  EL+MNS+K
Sbjct: 126 AVGGRDGRYYIVTDPSDHDPVTPKPGTLRYAVIQDEPLWIVFKRDMVITLSQELIMNSFK 185

Query: 212 TLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDG 271
           T+DGRG NVHI GG C+T+QY++N+IIH INIH C  TG+  VRSS +H G+R  +DGDG
Sbjct: 186 TIDGRGVNVHIAGGACLTVQYVTNIIIHGINIHDCKRTGNAMVRSSESHYGWRTMADGDG 245

Query: 272 ISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSG 331
           ISIF S +IWIDH SLS C DGLIDAIMGST ITISNNY +HH+E +LLGH D YT D  
Sbjct: 246 ISIFGSSHIWIDHNSLSSCADGLIDAIMGSTAITISNNYLTHHNEAILLGHTDSYTRDKM 305

Query: 332 MQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPG 391
           MQVTIA+N FGE L+QRMPRCR GY HVVNN++  WEMYAIGGS  PTINSQGNR++APG
Sbjct: 306 MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPG 365

Query: 392 NPNAKESTARILNAPLREIIYREVFGALSFFSNFFKILILAISDNVGAKLKILKYTRTKC 451
           N  AKE                                                      
Sbjct: 366 NRFAKE------------------------------------------------------ 425

Query: 452 KICKVNDLTGQSSATGFLFWTTCVTKRVDTNAGDWSEWNWRTEGDIMVNGAFFVPSGEGL 511
                                  VTKRV    G+W+ WNWR++GD+M+NGA+F  SG G 
Sbjct: 426 -----------------------VTKRVGAGKGEWNNWNWRSQGDLMLNGAYFTSSGAGA 428

Query: 512 SNMYVKASSVAPKSAALVDQLTLNAG 538
           S  Y +ASS+A KS++LV  LT ++G
Sbjct: 486 SANYARASSLAAKSSSLVGMLTSSSG 428

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SCP21.9e-15555.90Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2[more]
Q93Z044.3e-14751.83Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1[more]
Q9SVQ61.6e-13353.36Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2[more]
Q944R12.1e-13354.72Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1[more]
Q940Q18.8e-13253.46Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1H1R23.6e-26979.68Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111459254 PE=3 SV=1[more]
A0A6J1JIE02.4e-26578.71Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111487228 PE=3 SV=1[more]
A0A5D3BPB89.1e-24170.90Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00090 ... [more]
A0A1S3C4R49.1e-24170.90Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103496461 PE=3 SV=1[more]
A0A6J1FLS31.5e-23871.50Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111445375 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022957835.17.3e-26979.68probable pectate lyase 12 [Cucurbita moschata] >KAG7032968.1 putative pectate ly... [more]
XP_023524526.14.5e-26678.87probable pectate lyase 12 [Cucurbita pepo subsp. pepo][more]
XP_022990332.14.9e-26578.71probable pectate lyase 12 [Cucurbita maxima][more]
KAG6602287.16.9e-25979.00putative pectate lyase 12, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038884299.11.3e-24672.67probable pectate lyase 12 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G53190.11.4e-15655.90Pectin lyase-like superfamily protein [more]
AT5G04310.11.3e-15453.46Pectin lyase-like superfamily protein [more]
AT3G54920.13.1e-14851.83Pectin lyase-like superfamily protein [more]
AT4G13210.11.1e-13453.36Pectin lyase-like superfamily protein [more]
AT4G13210.21.1e-13453.36Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 144..161
score: 58.47
coord: 268..289
score: 65.15
coord: 370..389
score: 74.29
coord: 168..193
score: 63.74
coord: 204..220
score: 67.79
coord: 348..367
score: 62.86
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 196..393
e-value: 2.4E-89
score: 312.8
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 204..373
e-value: 7.3E-22
score: 78.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 466..540
e-value: 8.1E-6
score: 27.0
coord: 121..411
e-value: 2.9E-104
score: 350.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 542..564
NoneNo IPR availablePANTHERPTHR31683:SF122PECTATE LYASEcoord: 475..557
coord: 94..402
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 475..557
coord: 94..402
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 116..537

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G026670.1CmoCh04G026670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity