CmoCh04G025150 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh04G025150 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.3e-134
Identity = 264/608 (43.42%), Postives = 371/608 (61.02%), Query Frame = 0

Query: 35  CEEQCGDVHIPYPFGIKERCYL--NQNFSITCHKTDRNRPPKAEAFLMHTNIRVMNISTN 94
           C+ +CG+V + YPFG    CY   +++F++TC++ ++         L   N+ V+N+S +
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQEK---------LFFGNMPVINMSLS 88

Query: 95  GELHVLRPIVRNCYSYGDNYLD--MNETDLSVPTMYPLSRSKNKFITIGCNHVGLILGDD 154
           G+L V     R CY       D     T L   T+  L    N+F  +GCN     L   
Sbjct: 89  GQLRVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL----NRFTVVGCNSYA-FLRTS 148

Query: 155 QGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGLTKLSLAVGELLNYTEIRKFSPC 214
                 +GCIS+C   ++  +G CSG GCCQ+ +P+G + + +      N+  +  F+PC
Sbjct: 149 GVEKYSTGCISICD-SATTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPC 208

Query: 215 GYAFIIEARRFQF--LSSYIDKFEEKEVEAVLSWGI------RKALKLDCGLNARRNNSI 274
            YAF++E   F F  L    +         VL W I      +   +  CG N+   +S 
Sbjct: 209 TYAFLVEDGMFDFHALEDLNNLRNVTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDST 268

Query: 275 FNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFH 334
             GT Y C CL+G+EGNPYL +GCQD++EC     N  EH + C NT+G++ C+CP  + 
Sbjct: 269 -GGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEH-STCENTKGSFNCNCPSGYR 328

Query: 335 GDGRKGGKGCTKNSTSS----IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFH 394
            D       CT+           I +G  +G +V+++  + +    K  K  + +Q+FF 
Sbjct: 329 KDSL---NSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQKFFE 388

Query: 395 KNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDDGLT 454
           +NGG +L +++S        V+IF+++ +++ATN Y +  I G+GG GTVYKG+L D   
Sbjct: 389 QNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPDNSI 448

Query: 455 VAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTLFDH 514
           VAIKK++  + SQ  QFINEV+VLSQINHRNVVK+LGCCLET+VPLLVYEF+ +GTLFDH
Sbjct: 449 VAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTLFDH 508

Query: 515 IHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAKVSDF 574
           +H +     L+WE RLRIA+E AG ++YLHSSASIPIIHRDIKT N+LLD N TAKV+DF
Sbjct: 509 LHGSLYDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKVADF 568

Query: 575 GASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAVRFD 627
           GAS+L+P D  QLTT+VQGTLGYLDPEY  T  L EKSDVYSFG+VL+EL++G+KA+ F+
Sbjct: 569 GASRLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCFE 616

BLAST of CmoCh04G025150 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 475.7 bits (1223), Expect = 1.4e-132
Identity = 278/636 (43.71%), Postives = 374/636 (58.81%), Query Frame = 0

Query: 11  FLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYL--NQNFSITCHKTD 70
           FLV +  ++    L     Q    C+ +CG++ I YPFGI   CY   N++FSITC K D
Sbjct: 8   FLVAIFFSLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITC-KED 67

Query: 71  RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL 130
           R          + ++I V N + +G+L VL      CY   D      E D S  T+  L
Sbjct: 68  RPH--------VLSDIEVANFNHSGQLQVLLNRSSTCY---DEQGKKTEEDSSF-TLENL 127

Query: 131 SRS-KNKFITIGCNHVGLILGDDQG-TNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPK 190
           S S  NK   +GCN + L+  D  G  N  + C+S+C       DG C+G GCC++D+  
Sbjct: 128 SLSANNKLTAVGCNALSLL--DTFGMQNYSTACLSLCDSPPE-ADGECNGRGCCRVDVSA 187

Query: 191 GLTKLSLAV--GELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEV---EAVLS 250
            L   +     G + + T    FSPC YAF++E  +F F SS  D    + V     +L 
Sbjct: 188 PLDSYTFETTSGRIKHMTSFHDFSPCTYAFLVEDDKFNF-SSTEDLLNLRNVMRFPVLLD 247

Query: 251 WGIRKALKLDCGLNA--RRNNSIFNGT---HYRCHCLDGYEGNPYLHHGCQDVDECTYP- 310
           W +        G  +    N++  + T    Y C C +G++GNPYL  GCQDV+ECT   
Sbjct: 248 WSVGNQTCEQVGSTSICGGNSTCLDSTPRNGYICRCNEGFDGNPYLSAGCQDVNECTTSS 307

Query: 311 --WLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVL 370
               ++C     C N  G + C C   +  D       C +   +   I++   +G  V+
Sbjct: 308 TIHRHNCSDPKTCRNKVGGFYCKCQSGYRLDTTT--MSCKRKEFAWTTILLVTTIGFLVI 367

Query: 371 LIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNN 430
           L+    I    K LK  K +++FF +NGG +L ++LS        V+IF+++ ++KATN 
Sbjct: 368 LLGVACIQQRMKHLKDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNG 427

Query: 431 YAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKL 490
           YA+  I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV+VLSQINHRNVVKL
Sbjct: 428 YAESRILGQGGQGTVYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKL 487

Query: 491 LGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASI 550
           LGCCLET+VPLLVYEF+TNGTLFDH+H +     L+WE RL+IA E AG ++YLHSSASI
Sbjct: 488 LGCCLETEVPLLVYEFITNGTLFDHLHGSMIDSSLTWEHRLKIAIEVAGTLAYLHSSASI 547

Query: 551 PIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELT 610
           PIIHRDIKT N+LLD N TAKV+DFGAS+L+P D  +L T+VQGTLGYLDPEY  T  L 
Sbjct: 548 PIIHRDIKTANILLDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLN 607

Query: 611 EKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMY 630
           EKSDVYSFG+VL+EL++G+KA+ F  P+  ++L  Y
Sbjct: 608 EKSDVYSFGVVLMELLSGQKALCFKRPQSSKHLVSY 624

HSP 2 Score: 416.0 bits (1068), Expect = 1.3e-114
Identity = 214/413 (51.82%), Postives = 286/413 (69.25%), Query Frame = 0

Query: 633  VDECTYP---WLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIG 692
            V+ECT       ++C     C N  G + C C   +  D       C +   +   I++ 
Sbjct: 283  VNECTTSSTIHRHNCSDPKTCRNKVGGFYCKCQSGYRLDTTT--MSCKRKEFAWTTILLV 342

Query: 693  IGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQE 752
              +G  V+L+    I    K LK  K +++FF +NGG +L ++LS        V+IF+++
Sbjct: 343  TTIGFLVILLGVACIQQRMKHLKDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTED 402

Query: 753  ELEKATNNYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQI 812
             ++KATN YA+  I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV+VLSQI
Sbjct: 403  GMKKATNGYAESRILGQGGQGTVYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQI 462

Query: 813  NHRNVVKLLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVIS 872
            NHRNVVKLLGCCLET+VPLLVYEF+TNGTLFDH+H +     L+WE RL+IA E AG ++
Sbjct: 463  NHRNVVKLLGCCLETEVPLLVYEFITNGTLFDHLHGSMIDSSLTWEHRLKIAIEVAGTLA 522

Query: 873  YLHSSASIPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPE 932
            YLHSSASIPIIHRDIKT N+LLD N TAKV+DFGAS+L+P D  +L T+VQGTLGYLDPE
Sbjct: 523  YLHSSASIPIIHRDIKTANILLDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPE 582

Query: 933  YLLTSELTEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEDRFEEVVEK 992
            Y  T  L EKSDVYSFG+VL+EL++G+KA+ F  P+  ++L  Y   A KE+R +E++  
Sbjct: 583  YYNTGLLNEKSDVYSFGVVLMELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGG 642

Query: 993  GMVSEANMEQIKEAAKLARECVRIKGEERPSMKEVAMELEALRATKGEHSWAN 1043
             +++E N+++I+EAA++A EC R+ GEERP MKEVA +LEALR  K +H W++
Sbjct: 643  EVMNEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSD 693

BLAST of CmoCh04G025150 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 475.3 bits (1222), Expect = 1.8e-132
Identity = 277/646 (42.88%), Postives = 374/646 (57.89%), Query Frame = 0

Query: 11  FLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYL--NQNFSITCHKTD 70
           FLV++    ++  L     Q    C+ +CG+V I YPFGI   CY   + NF++TC    
Sbjct: 8   FLVVIFFLAYT-QLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC---- 67

Query: 71  RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL 130
                  E  L+   I+V NIS +G + VL      CY    N  +       + + + L
Sbjct: 68  ----VVEEKLLLFGIIQVTNISHSGHVSVLFERFSECYEQ-KNETNGTALGYQLGSSFSL 127

Query: 131 SRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLD----- 190
           S S NKF  +GCN + L L      N  +GC+S+C  +    +G C+G GCC  +     
Sbjct: 128 S-SNNKFTLVGCNALSL-LSTFGKQNYSTGCLSLCNSQPE-ANGRCNGVGCCTTEDFSVP 187

Query: 191 -----IPKGLTKLSLAVGELLNY--TEIRKFSPCGYAFIIEARRFQFLSSYIDK--FEEK 250
                   G  +L   V   L+   T + +F+PC YAF++E  +F F SS   K      
Sbjct: 188 FDSDTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVT 247

Query: 251 EVEAVLSWGIRKALKLDCGLN--ARRNNSIFNGT---HYRCHCLDGYEGNPYLHHGCQDV 310
                L W I        G      +N+S +N T    Y C C +GY+GNPY   GC+D+
Sbjct: 248 RFPVALDWSIGNQTCEQAGSTRICGKNSSCYNSTTRNGYICKCNEGYDGNPYRSEGCKDI 307

Query: 311 DECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLG 370
           DEC     ++C     C N +G + C CP    G        CT+       I + I +G
Sbjct: 308 DECISD-THNCSDPKTCRNRDGGFDCKCPS---GYDLNSSMSCTRPEYKRTRIFLVIIIG 367

Query: 371 LAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEK 430
           + VLL+A   I    K+ K+ K +++FF +NGG +L ++LS         +IF++E +++
Sbjct: 368 VLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKE 427

Query: 431 ATNNYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRN 490
           ATN Y +  I G+GG GTVYKG+L D   VAIKK++  D  Q  QFI+EV+VLSQINHRN
Sbjct: 428 ATNGYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRN 487

Query: 491 VVKLLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHS 550
           VVK+LGCCLET+VPLLVYEF+TNGTLFDH+H +     L+WE RLRIA E AG ++YLHS
Sbjct: 488 VVKILGCCLETEVPLLVYEFITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYLHS 547

Query: 551 SASIPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLT 610
           SASIPIIHRDIKT N+LLD+N TAKV+DFGASKL+P D  QLTT+VQGTLGYLDPEY  T
Sbjct: 548 SASIPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYTT 607

Query: 611 SELTEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMYVLYVDE 636
             L EKSDVYSFG+VL+EL++G+KA+ F+ P+  ++L  Y +   E
Sbjct: 608 GLLNEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATE 636

HSP 2 Score: 426.0 bits (1094), Expect = 1.3e-117
Identity = 215/410 (52.44%), Postives = 290/410 (70.73%), Query Frame = 0

Query: 633  VDECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGL 692
            +DEC     ++C     C N +G + C CP    G        CT+       I + I +
Sbjct: 294  IDECISD-THNCSDPKTCRNRDGGFDCKCPS---GYDLNSSMSCTRPEYKRTRIFLVIII 353

Query: 693  GLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELE 752
            G+ VLL+A   I    K+ K+ K +++FF +NGG +L ++LS         +IF++E ++
Sbjct: 354  GVLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMK 413

Query: 753  KATNNYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHR 812
            +ATN Y +  I G+GG GTVYKG+L D   VAIKK++  D  Q  QFI+EV+VLSQINHR
Sbjct: 414  EATNGYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHR 473

Query: 813  NVVKLLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLH 872
            NVVK+LGCCLET+VPLLVYEF+TNGTLFDH+H +     L+WE RLRIA E AG ++YLH
Sbjct: 474  NVVKILGCCLETEVPLLVYEFITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYLH 533

Query: 873  SSASIPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLL 932
            SSASIPIIHRDIKT N+LLD+N TAKV+DFGASKL+P D  QLTT+VQGTLGYLDPEY  
Sbjct: 534  SSASIPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYT 593

Query: 933  TSELTEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMV 992
            T  L EKSDVYSFG+VL+EL++G+KA+ F+ P+  ++L  Y + A +E+R  E+++  ++
Sbjct: 594  TGLLNEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQVL 653

Query: 993  SEANMEQIKEAAKLARECVRIKGEERPSMKEVAMELEALRATKGEHSWAN 1043
            +E N+++I+EAA++A EC R+ GEERP MKEVA +LEALR  K +H W++
Sbjct: 654  NEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSD 699

BLAST of CmoCh04G025150 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.8e-130
Identity = 268/636 (42.14%), Postives = 369/636 (58.02%), Query Frame = 0

Query: 12  LVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYL--NQNFSITCHKTDR 71
           L +M I  + A   +  +Q    C+ +CGDV I YPFGI   CY   + +F+ITC +   
Sbjct: 6   LFLMAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEEDKP 65

Query: 72  NRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPLS 131
           N         + +NI V+N + +G+L  L P    CY    N    N+ +     +  LS
Sbjct: 66  N---------VLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTN----NDFESLWFRLDNLS 125

Query: 132 RS-KNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCC--QLDIPK 191
            S  NKF  +GCN    +L      N  +GC+S+C       +  C+G GCC  ++ IP 
Sbjct: 126 FSPNNKFTLVGCN-AWALLSTFGIQNYSTGCMSLCD-TPPPPNSKCNGVGCCRTEVSIPL 185

Query: 192 GLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQF--LSSYIDKFEEKEVEAVLSWGI 251
              ++        N T +  F+PC YAF +E   F F  L    D         +L W I
Sbjct: 186 DSHRIETQPSRFENMTSVEHFNPCSYAFFVEDGMFNFSSLEDLKDLRNVTRFPVLLDWSI 245

Query: 252 -RKALKLDCGLNARRNNSI----FNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLND 311
             +  +   G N    NS       G  Y C CL G++GNPYL  GCQD++ECT   +++
Sbjct: 246 GNQTCEQVVGRNICGGNSTCFDSTRGKGYNCKCLQGFDGNPYLSDGCQDINECT-TRIHN 305

Query: 312 CEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSS------IPIIIGIGLGLAVL 371
           C   + C NT G++ C CP     D       C               +++G  +G  ++
Sbjct: 306 CSDTSTCENTLGSFHCQCPSG--SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLII 365

Query: 372 LIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNN 431
           L+  + I    +  K  + +Q+FF +NGG +L ++LS        V+IF++E +++AT+ 
Sbjct: 366 LLTISYIQQKMRHRKNTELRQQFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDG 425

Query: 432 YAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKL 491
           Y +  I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV+VLSQINHRNVVKL
Sbjct: 426 YNESRILGQGGQGTVYKGILQDNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKL 485

Query: 492 LGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASI 551
           LGCCLET+VPLLVYEF+++GTLFDH+H +     L+WE RLRIA E AG ++YLHS ASI
Sbjct: 486 LGCCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRIAIEVAGTLAYLHSYASI 545

Query: 552 PIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELT 611
           PIIHRD+KT N+LLD+N TAKV+DFGAS+L+P D  QLTT+VQGTLGYLDPEY  T  L 
Sbjct: 546 PIIHRDVKTANILLDENLTAKVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLN 605

Query: 612 EKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMY 630
           EKSDVYSFG+VL+EL++G+KA+ F+ P+  ++L  Y
Sbjct: 606 EKSDVYSFGVVLMELLSGEKALCFERPQSSKHLVSY 623

BLAST of CmoCh04G025150 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 7.1e-129
Identity = 268/637 (42.07%), Postives = 379/637 (59.50%), Query Frame = 0

Query: 17  IAIFS-APLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTDRNRPPKA 76
           +AIF  + + +   Q LP C E+CG+V + YPFG    C+  ++ S      + N     
Sbjct: 9   VAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCVNEN----- 68

Query: 77  EAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPLSRSKNKF 136
              L +  + V+ IS + +L VL P    CY+    +    +       +  L+ S N  
Sbjct: 69  ---LFYKGLEVVEISHSSQLRVLYPASYICYNSKGKFA---KGTYYWSNLGNLTLSGNNT 128

Query: 137 IT-IGCNHVGLILGDDQGTNVES-GCISVCTRRSSVVDGLCSGSGCCQLDIPKGLTKLSL 196
           IT +GCN    +     GT   S GCIS C   S   +G C+G GCCQ  +P G   L +
Sbjct: 129 ITALGCNSYAFV--SSNGTRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIV 188

Query: 197 AVGELLNYTEIRKFS--PCGYAFIIEARRFQFLSSYIDKFEEKE-----VEAVLSWGIR- 256
                 N T ++  S   C YAF++E  +F++ +S  DK+   +        VL W IR 
Sbjct: 189 RSYRFDNDTSVQPISEGQCIYAFLVENGKFKYNAS--DKYSYLQNRNVGFPVVLDWSIRG 248

Query: 257 ----KALKLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYP---WLN 316
               +  +  CG+N   +NS  +G  Y C C  G++GNPYL +GCQD++ECT       +
Sbjct: 249 ETCGQVGEKKCGVNGICSNSA-SGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKH 308

Query: 317 DCEHKNKCSNTEGNYTCHCPKNFHGDGR------KGGKGCTKNSTSSIPIIIGIGLGLAV 376
           +C   + C N  G++ C+C   +  +        KG     + +T    I++G  +G  V
Sbjct: 309 NCSGDSTCENKLGHFRCNCRSRYELNTTTNTCKPKGNPEYVEWTT----IVLGTTIGFLV 368

Query: 377 LLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATN 436
           +L+A + I    K  K  + +Q+FF +NGG +L ++LS        V+IF++E +++AT+
Sbjct: 369 ILLAISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATD 428

Query: 437 NYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVK 496
            Y ++ I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV+VLSQINHRNVVK
Sbjct: 429 GYDENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVK 488

Query: 497 LLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSAS 556
           LLGCCLET+VPLLVYEF+++GTLFDH+H +     L+WE RLR+A E AG ++YLHSSAS
Sbjct: 489 LLGCCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRMAVEIAGTLAYLHSSAS 548

Query: 557 IPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSEL 616
           IPIIHRDIKT N+LLD+N TAKV+DFGAS+L+P D   L T+VQGTLGYLDPEY  T  L
Sbjct: 549 IPIIHRDIKTANILLDENLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLL 608

Query: 617 TEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMY 630
            EKSDVYSFG+VL+EL++G+KA+ F+ P+  +++  Y
Sbjct: 609 NEKSDVYSFGVVLMELLSGQKALCFERPQTSKHIVSY 625

HSP 2 Score: 407.5 bits (1046), Expect = 4.6e-112
Identity = 211/419 (50.36%), Postives = 293/419 (69.93%), Query Frame = 0

Query: 633  VDECTYP---WLNDCEHKNKCSNTEGNYTCHCPKNFHGDGR------KGGKGCTKNSTSS 692
            ++ECT       ++C   + C N  G++ C+C   +  +        KG     + +T  
Sbjct: 280  INECTTANPIHKHNCSGDSTCENKLGHFRCNCRSRYELNTTTNTCKPKGNPEYVEWTT-- 339

Query: 693  IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIV 752
              I++G  +G  V+L+A + I    K  K  + +Q+FF +NGG +L ++LS        V
Sbjct: 340  --IVLGTTIGFLVILLAISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDV 399

Query: 753  RIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEV 812
            +IF++E +++AT+ Y ++ I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV
Sbjct: 400  KIFTEEGMKEATDGYDENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEV 459

Query: 813  VVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASE 872
            +VLSQINHRNVVKLLGCCLET+VPLLVYEF+++GTLFDH+H +     L+WE RLR+A E
Sbjct: 460  LVLSQINHRNVVKLLGCCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRMAVE 519

Query: 873  TAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTL 932
             AG ++YLHSSASIPIIHRDIKT N+LLD+N TAKV+DFGAS+L+P D   L T+VQGTL
Sbjct: 520  IAGTLAYLHSSASIPIIHRDIKTANILLDENLTAKVADFGASRLIPMDKEDLATMVQGTL 579

Query: 933  GYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEDRF 992
            GYLDPEY  T  L EKSDVYSFG+VL+EL++G+KA+ F+ P+  +++  Y   A KE+R 
Sbjct: 580  GYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCFERPQTSKHIVSYFASATKENRL 639

Query: 993  EEVVEKGMVSEANMEQIKEAAKLARECVRIKGEERPSMKEVAMELEALRATKGEHSWAN 1043
             E+++  +++E N  +I++AA++A EC R+ GEERP MKEVA ELEALR TK +H W++
Sbjct: 640  HEIIDGQVMNENNQREIQKAARIAVECTRLTGEERPGMKEVAAELEALRVTKTKHKWSD 694

BLAST of CmoCh04G025150 vs. ExPASy TrEMBL
Match: A0A6J1H8D6 (uncharacterized protein LOC111460588 OS=Cucurbita moschata OX=3662 GN=LOC111460588 PE=4 SV=1)

HSP 1 Score: 2154.0 bits (5580), Expect = 0.0e+00
Identity = 1067/1078 (98.98%), Postives = 1068/1078 (99.07%), Query Frame = 0

Query: 9    MAFLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTD 68
            MAFLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTD
Sbjct: 1    MAFLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTD 60

Query: 69   RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL 128
            RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL
Sbjct: 61   RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL 120

Query: 129  SRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGL 188
            SRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGL
Sbjct: 121  SRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGL 180

Query: 189  TKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWGIRKAL 248
            TKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWGIRKAL
Sbjct: 181  TKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWGIRKAL 240

Query: 249  KLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSN 308
            KLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSN
Sbjct: 241  KLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSN 300

Query: 309  TEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLK 368
            TEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLK
Sbjct: 301  TEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLK 360

Query: 369  FIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTV 428
            FIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTV
Sbjct: 361  FIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTV 420

Query: 429  YKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYE 488
            YKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYE
Sbjct: 421  YKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYE 480

Query: 489  FVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLD 548
            FVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLD
Sbjct: 481  FVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLD 540

Query: 549  DNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLEL 608
            DNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLEL
Sbjct: 541  DNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLEL 600

Query: 609  ITGKKAVRFDGPEEDRNLAMYVLY----------VDECTYPWLNDCEHKNKCSNTEGNYT 668
            ITGKKAVRFDGPEEDRNLAMYVL+          VDECTYPWLNDCEHKNKCSNTEGNYT
Sbjct: 601  ITGKKAVRFDGPEEDRNLAMYVLFLLGLFEPFLDVDECTYPWLNDCEHKNKCSNTEGNYT 660

Query: 669  CHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQ 728
            CHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQ
Sbjct: 661  CHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQ 720

Query: 729  RFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLD 788
            RFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLD
Sbjct: 721  RFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLD 780

Query: 789  DGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGT 848
            DGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGT
Sbjct: 781  DGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGT 840

Query: 849  LFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAK 908
            LFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAK
Sbjct: 841  LFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAK 900

Query: 909  VSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKA 968
            VSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKA
Sbjct: 901  VSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKA 960

Query: 969  VRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAKLARECVRIKGEER 1028
            VRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAKLARECVRIKGEER
Sbjct: 961  VRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAKLARECVRIKGEER 1020

Query: 1029 PSMKEVAMELEALRATKGEHSWANVRLSAAQESSPFVEGGSMGAVDDSIKLELPHVEH 1077
            PSMKEVAMELEALRATKGEHSWANVRLSAAQESSPFVEGGSMGAVDDSIKLELPHVEH
Sbjct: 1021 PSMKEVAMELEALRATKGEHSWANVRLSAAQESSPFVEGGSMGAVDDSIKLELPHVEH 1078

BLAST of CmoCh04G025150 vs. ExPASy TrEMBL
Match: A0A6J1H6B0 (uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC111460594 PE=4 SV=1)

HSP 1 Score: 1645.9 bits (4261), Expect = 0.0e+00
Identity = 943/1729 (54.54%), Postives = 1001/1729 (57.89%), Query Frame = 0

Query: 5    MKPIMAFLVM-MNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSIT 64
            MK IMAF+VM M IAI S  LA+ ASQALP C+++CGDVHIPYPFGIKE CYLNQNFSIT
Sbjct: 1    MKHIMAFMVMIMEIAILSTALAMVASQALPQCDDRCGDVHIPYPFGIKEGCYLNQNFSIT 60

Query: 65   CHKTDRNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVP 124
            C+KTDRN PPK  A+LM TNI V NISTNGELHVL+P+VR CY   +     NE++LSVP
Sbjct: 61   CNKTDRNGPPK--AYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPNESNLSVP 120

Query: 125  TMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLD 184
             M+ +S +KNKF+TIGCN + +I G+ QG+   SGCISVC  +SSVV+G CSG+GCCQL+
Sbjct: 121  AMFSISGTKNKFVTIGCNTLSVITGNHQGSLYGSGCISVCLNKSSVVNGPCSGNGCCQLE 180

Query: 185  IPKGLTKLSLAVGELLNYTEIRKFSPCGYAFII-EARRFQFLSSYIDKFEEKEVEAVLSW 244
             P GLT LS+AVG LLN+T++R F+PCGYAF+I E +RF+FLSSYI+ FE++EVE VL W
Sbjct: 181  FPNGLTNLSVAVGGLLNFTDVRTFNPCGYAFVIEEEKRFEFLSSYIENFEDEEVEVVLDW 240

Query: 245  GIRKALKLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEH 304
            GIR   + +CG N  R +SI +GT YRC CLDGY+GNPYL  GCQDVDEC Y WLNDCE+
Sbjct: 241  GIRNETRFECGSNTTR-SSILDGTEYRCQCLDGYDGNPYLPQGCQDVDECEYAWLNDCEY 300

Query: 305  KNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYL 364
            K+KC+NT+GNYTCHCPKNFHGDGR+GG+GCTKNSTSSIPIIIGIG+GL VLLIATTTIYL
Sbjct: 301  KDKCANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIIIGIGVGLPVLLIATTTIYL 360

Query: 365  CYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGK 424
             YKKLKFIKQKQ FFHKNGGFVLQRQLSQ NSP D+VRIFSQEELEKATNNYA+DTIAGK
Sbjct: 361  GYKKLKFIKQKQLFFHKNGGFVLQRQLSQWNSPNDMVRIFSQEELEKATNNYAQDTIAGK 420

Query: 425  GGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQV 484
            GG+GTVYKGVLDDGLT+AIKKSKFMDESQTSQFINEV+VLSQINHRNVVKLLGCCLETQV
Sbjct: 421  GGFGTVYKGVLDDGLTIAIKKSKFMDESQTSQFINEVIVLSQINHRNVVKLLGCCLETQV 480

Query: 485  PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKT 544
            PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKT
Sbjct: 481  PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKT 540

Query: 545  TNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFG 604
            TN+LLDDNYTAKVSDFGASKLVP D TQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFG
Sbjct: 541  TNILLDDNYTAKVSDFGASKLVPMDQTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFG 600

Query: 605  IVLLELITGKKAVRFDGPEEDRNLAMYVLY------------------------------ 664
            IVLLELITGKKAVRFDGPEEDRNLAMYVL                               
Sbjct: 601  IVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEERLEEVVEKGMVSEANMEQIKEAAKL 660

Query: 665  ------------------------------------------------------------ 724
                                                                        
Sbjct: 661  ARECVRIKGEERPSMKEVAMELEGLRATKGEHSWANVNLSSAEETVYLLDGASESSQLVG 720

Query: 725  ------------------------------------------------------------ 784
                                                                        
Sbjct: 721  SGSVGTVGNSIKLEITLMEHGRRVVQNCYRDAFTRVSNLSVPDMYPHSRSKNKFITIGCN 780

Query: 785  ------------------------------------------------------------ 844
                                                                        
Sbjct: 781  HVGLILGAYQGSDFESRCISMCTKNSSVVDESCSGSGCCQLEIPDGLSNLRLAVGELLHS 840

Query: 845  ------------------------------------------------------------ 904
                                                                        
Sbjct: 841  TDVWKFNPCGYAFIIEAERFKFLSSFIKNFKDKEVEVVLGWGIRNELELDCGLNARRNSS 900

Query: 905  ------------------------------------------------------------ 964
                                                                        
Sbjct: 901  IFNGTHYRCQCLDGYEGNPYLERMSRCLQATDQGRGAFESCAVTEGFESCAVTEGIVQCQ 960

Query: 965  ------------------------------------------------------------ 1024
                                                                        
Sbjct: 961  SIFRCRSLFWNAHTPTFCDELFNIFTIYTHQQQPCPLMVMNIKPIMAFMVMMMKIAILST 1020

Query: 1025 ------------------------------------------------------------ 1079
                                                                        
Sbjct: 1021 ALAVAASQALPGCDEWCGKVHIPYPFGIKKGCYLNQNFSITCHKTDRNRPPKALLMHTNI 1080

BLAST of CmoCh04G025150 vs. ExPASy TrEMBL
Match: A0A6J1JGR1 (wall-associated receptor kinase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111486860 PE=4 SV=1)

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 687/1079 (63.67%), Postives = 708/1079 (65.62%), Query Frame = 0

Query: 1    MVMKMKPIMAFLV-MMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQN 60
            MVM +KPIMAF+V MM I I S  LAV AS+ALPGC+E CG VHIPYPFGIK+ CYLNQN
Sbjct: 1    MVMNIKPIMAFMVMMMKIGILSTALAVEASEALPGCDEWCGKVHIPYPFGIKKGCYLNQN 60

Query: 61   FSITCHKTDRNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETD 120
            FSITCHKTD N PPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETD
Sbjct: 61   FSITCHKTDLNGPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETD 120

Query: 121  LSVPTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGC 180
            LSVPTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRR SVVDGLCSGSGC
Sbjct: 121  LSVPTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRISVVDGLCSGSGC 180

Query: 181  CQLDIPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAV 240
            CQL+IPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVE V
Sbjct: 181  CQLEIPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEVV 240

Query: 241  LSWGIRKALKLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLND 300
            LSWGIRK L+LDCGLNARRN+SIFNGTHYRC CLDGYEGNPYL HGCQD           
Sbjct: 241  LSWGIRKELELDCGLNARRNSSIFNGTHYRCQCLDGYEGNPYLRHGCQD----------- 300

Query: 301  CEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTT 360
                                                                        
Sbjct: 301  ------------------------------------------------------------ 360

Query: 361  IYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTI 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  AGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLE 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  TQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRD 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  IKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVY 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  SFGIVLLELITGKKAVRFDGPEEDRNLAMYVLYVDECTYPWLNDCEHKNKCSNTEGNYTC 660
                                             VDEC YPWLNDC+HK+KCSNTEGNYTC
Sbjct: 601  ---------------------------------VDECKYPWLNDCKHKDKCSNTEGNYTC 660

Query: 661  HCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQR 720
            HCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIG+GL VLLIATTTIYL YKKLKFIKQKQ 
Sbjct: 661  HCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGVGLPVLLIATTTIYLGYKKLKFIKQKQL 720

Query: 721  FFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDD 780
            FF KNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYA+DTIAGKGGYGTV+KGVLDD
Sbjct: 721  FFQKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAQDTIAGKGGYGTVFKGVLDD 735

Query: 781  GLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTL 840
            GLT+AIKKSKFMDESQTSQFINEV+VLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTL
Sbjct: 781  GLTIAIKKSKFMDESQTSQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTL 735

Query: 841  FDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAKV 900
            FDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTN+LLD+NYTAKV
Sbjct: 841  FDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNILLDNNYTAKV 735

Query: 901  SDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAV 960
            SDFGASKLVPKD TQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIV+LELITGKKAV
Sbjct: 901  SDFGASKLVPKDQTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVVLELITGKKAV 735

Query: 961  RFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAKLARECVRIKGEERP 1020
            RFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKE AK+ARECVRIKGEERP
Sbjct: 961  RFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEVAKVARECVRIKGEERP 735

Query: 1021 SMKEVAMELEALRATKGEHSWANVRLSAAQESSPFVEGGSMGAVDDSIKLELPHVEHGR 1079
            SMKEVAMELEALRATKGEHSWANVRLSAA ESSPFVE GS+GAVDDS+KLELPHVEHGR
Sbjct: 1021 SMKEVAMELEALRATKGEHSWANVRLSAAGESSPFVESGSIGAVDDSMKLELPHVEHGR 735

BLAST of CmoCh04G025150 vs. ExPASy TrEMBL
Match: A0A6J1H843 (wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111461403 PE=4 SV=1)

HSP 1 Score: 1241.1 bits (3210), Expect = 0.0e+00
Identity = 706/1449 (48.72%), Postives = 837/1449 (57.76%), Query Frame = 0

Query: 16   NIAIFS----APLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTDRNR 75
            NI I S    A  AVAA QALPGC+++CGD+ IPYPFG +E CYLN+NF ITC+ T  +R
Sbjct: 4    NILILSPLVYAATAVAAFQALPGCDDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHDDR 63

Query: 76   PPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTM-YPLSR 135
            P     FL   NI+V NIS +GELH+   + ++CY    N L    +  ++  + + +S 
Sbjct: 64   P---TPFLRTGNIQVTNISISGELHISNVVAKDCYP-KSNSLKARPSSATLNLLEFTVSS 123

Query: 136  SKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGLTK 195
            +KNKF  IGC+    + G  +G    +GC+++C   ++V DG CSG+GCCQLDIP GL  
Sbjct: 124  TKNKFTVIGCDTYAYLSGLIEGQTYTTGCMALCDNITTVRDGACSGNGCCQLDIPSGLKG 183

Query: 196  LSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWGIRKALKL 255
            L   V    N++++  ++PCGYAF+ E  +F F  +YI  F +K V AVL WGI     L
Sbjct: 184  LRCRVQSFKNHSKVLSYNPCGYAFVTEEDKFNFSGAYIRNFTQKRVPAVLDWGISNTTCL 243

Query: 256  D--------CGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEH 315
                     CG N+ +  S+ +G+ YRC CL+G+EGNPYL  GCQD+DEC    LNDC  
Sbjct: 244  TANNESNCVCGPNSMKVTSLPDGSEYRCGCLEGFEGNPYLPQGCQDIDECRDERLNDC-- 303

Query: 316  KNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYL 375
            K +C NTEGNYTC+CP+ F GDGR+ G+GCT++S S + +IIG+ +G  VL+I +T +YL
Sbjct: 304  KFECVNTEGNYTCNCPEGFKGDGRRRGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYL 363

Query: 376  CYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGK 435
             Y+K K IK K++FF ++GG +LQR LSQ  S  D+V IF+QEEL+KATN Y +  + GK
Sbjct: 364  GYRKWKLIKLKEKFFEEHGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGK 423

Query: 436  GGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQV 495
            GGYGTVYKG+L DG  VAIKKSK +D+SQTSQFINEV+VLSQINHRNVV+L+GCCLETQV
Sbjct: 424  GGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVRLVGCCLETQV 483

Query: 496  PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKT 555
            PLLVYEF+TNGTLFDHIHDTTKHVPLSW+ARLRIASETAGVISYLHSSASIPIIHRDIKT
Sbjct: 484  PLLVYEFITNGTLFDHIHDTTKHVPLSWKARLRIASETAGVISYLHSSASIPIIHRDIKT 543

Query: 556  TNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFG 615
            TN+LLDDNY AKVSDFGASKLVP D TQL+T+VQGT GYLDPEYLLTSELTEKSDVYSFG
Sbjct: 544  TNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTFGYLDPEYLLTSELTEKSDVYSFG 603

Query: 616  IVLLELITGKKAVRFDGPEEDRNLAMYVLY------------------------------ 675
            IVLLELITGKKA  F+GPE +RNLA+YVL                               
Sbjct: 604  IVLLELITGKKAGNFEGPEAERNLAVYVLRAMKEDRLGDVVEKGMAREREFEQIKEVGKV 663

Query: 676  ------------------------------------------------------------ 735
                                                                        
Sbjct: 664  ARKCLRISGEERPSMKEVGMELEGLRVMVEHEWVDEEKLVADGALDSTSNQFRQIETLIR 723

Query: 736  ------------------------------------------------------------ 795
                                                                        
Sbjct: 724  LMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHFNPPR 783

Query: 796  ------------------------------------------------------------ 855
                                                                        
Sbjct: 784  PFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTVSSTKNKFT 843

Query: 856  ------------------------------------------------------------ 915
                                                                        
Sbjct: 844  VIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLRYRVR 903

Query: 916  ------------------------------------------------------------ 975
                                                                        
Sbjct: 904  SFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTTCSTANNKS 963

Query: 976  ----------------------------------------VDECTYPWLNDCEHKNKCSN 1035
                                                    +DEC    LNDC  K +C N
Sbjct: 964  NCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDERLNDC--KFECVN 1023

Query: 1036 TEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLK 1079
            T+GNYTC+CP+ F GDGR+GG+GCT++S S + +IIG+ +G  VL+I +T +YL Y+K K
Sbjct: 1024 TKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYRKWK 1083

BLAST of CmoCh04G025150 vs. ExPASy TrEMBL
Match: F6H0G0 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g01370 PE=4 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 580/1055 (54.98%), Postives = 740/1055 (70.14%), Query Frame = 0

Query: 22   APLAVAASQAL-PGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTDRNRPPKAEAFLM 81
            A  A AASQ + P CE  CGDV IPYPFG +E CYLN +F I C+ +    PPK   +  
Sbjct: 714  AAAAKAASQTIKPDCEATCGDVSIPYPFGTREGCYLNDDFLIACNHS--LSPPKPLLWNS 773

Query: 82   HTNIRVMNIS-TNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPLSRSKNKFITIG 141
              N++V+NIS  +  L +   + R+CY       D      +    +P S   N+F  IG
Sbjct: 774  SFNLQVLNISIEDHRLRIYTFVGRDCYDKMGKQYDQPTLAYANLPRFPFSDKGNRFTAIG 833

Query: 142  CNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQL-DIPKGLTKLSLAVGEL 201
            C+ + +  G +   +  +GC+S+C    SV +G CSG GCCQ  +IPKGL     +VG  
Sbjct: 834  CDTIAVFNGLNGADDFTTGCLSLCNSIRSVTNGSCSGIGCCQTSNIPKGLFSYYASVGSF 893

Query: 202  LNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEV-EAVLSWGI---------RKAL 261
             N+T++  F+PC YAF+ E   F F S+ +   + + V   +L W +         +   
Sbjct: 894  YNHTKVWSFNPCSYAFLAEEESFNFSSADLKDLQNRTVFPTLLDWAVGNKTCEEAKKNLT 953

Query: 262  KLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSN 321
               C  N+   NS  NG  YRC+C  G++GNPYL +GCQD+DEC  P  N+C     C N
Sbjct: 954  SYACKDNSYCYNS-DNGPGYRCNCSSGFQGNPYLPNGCQDIDECADPKRNEC--TKVCIN 1013

Query: 322  TEGNYTCHCPKNFHGDGRK--GGKGCTKNSTS--SIPIIIGIGLGLAVLLIATTTIYLCY 381
            T G+YTC CPK +HG+GR+   G GCT +      + I +GI +GL  LLI ++ +Y   
Sbjct: 1014 TPGSYTCSCPKGYHGNGRRDENGDGCTPHDDQLLIVKIAVGIFIGLIALLITSSWLYWGL 1073

Query: 382  KKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGG 441
            KK KFIK K++FF +NGG +LQ+QL       + V+IF+ EELEKATN Y +DTI G+GG
Sbjct: 1074 KKRKFIKLKEKFFQQNGGLMLQQQLHGREGSSESVKIFTAEELEKATNKYDEDTIIGRGG 1133

Query: 442  YGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPL 501
            YGTVYKG+L DG  VAIKKSK +D++Q  QFINEVVVLSQINHRNVVKLLGCCLET+VPL
Sbjct: 1134 YGTVYKGILADGRVVAIKKSKLVDQTQIEQFINEVVVLSQINHRNVVKLLGCCLETEVPL 1193

Query: 502  LVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTN 561
            LVYEF+TNGTLFD+IH+ +K   +SWE RLRIA+ETAGV+SYLHSSASIPIIHRD+K+TN
Sbjct: 1194 LVYEFITNGTLFDYIHNKSKASSISWETRLRIAAETAGVLSYLHSSASIPIIHRDVKSTN 1253

Query: 562  VLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIV 621
            +LLDDNYTAKVSDFGAS+LVP D TQL+T+VQGTLGYLDPEYL TS+LTEKSDVYSFG+V
Sbjct: 1254 ILLDDNYTAKVSDFGASRLVPLDQTQLSTMVQGTLGYLDPEYLHTSQLTEKSDVYSFGVV 1313

Query: 622  LLELITGKKAVRFDGPEEDRNLAMYVL--YVDECTYPWLNDCEHKNKCSNTEGNYTCHCP 681
            L+EL+TGKKA+ FD PEE+R+LAM+ L    ++  +  L D    N  +  +        
Sbjct: 1314 LVELLTGKKALSFDRPEEERSLAMHFLSSLKNDRLFQILEDYIVPNDENMEQLKDVAKLA 1373

Query: 682  K---NFHGDGR----------KGGKGCTKNSTSSIPI----IIGIGLGLAVLLIATTTIY 741
            K      G+ R           G +  TK+   +I +       +G+GL  LLI ++ +Y
Sbjct: 1374 KRCLEVKGEERPTMKEVARELDGMRMMTKHPWVNIELNPEETECVGIGLISLLIGSSWLY 1433

Query: 742  LCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAG 801
               KK KFIK K+ FF +NGG +LQ+QLS+     + ++IF+  ELEKATN Y +  I G
Sbjct: 1434 WGLKKRKFIKLKEEFFQQNGGLMLQKQLSKREGSTETIKIFTGAELEKATNKYNESKIIG 1493

Query: 802  KGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQ 861
             GGYGTVYKG L DG  VAIKKSK +D+SQ  QFINEV+VLSQINHRNVVKLLGCCLET+
Sbjct: 1494 HGGYGTVYKGTLTDGRIVAIKKSKMVDKSQIEQFINEVLVLSQINHRNVVKLLGCCLETK 1553

Query: 862  VPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIK 921
            VPLLVYEF+TNGTLFDHIH+ +    + WE RLRIA+ETAGV+SYLHS+ASIPIIHRD+K
Sbjct: 1554 VPLLVYEFITNGTLFDHIHNKSNTSIIPWEIRLRIATETAGVLSYLHSAASIPIIHRDVK 1613

Query: 922  TTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSF 981
            +TN+LLDDNYTAKVSDFGAS+LVP D TQL+T+VQGTLGYLDPEYLLTS+LTEKSDVYSF
Sbjct: 1614 STNILLDDNYTAKVSDFGASRLVPLDQTQLSTMVQGTLGYLDPEYLLTSQLTEKSDVYSF 1673

Query: 982  GIVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAK 1041
            G+VL+EL+TG+KA+ FD PE+ R+LAMY L ++++DR  +V+++ +V+E N+EQ+KEAAK
Sbjct: 1674 GVVLVELLTGEKALSFDRPEDKRSLAMYFLFSLRDDRLFQVLDEHIVNEENIEQLKEAAK 1733

BLAST of CmoCh04G025150 vs. NCBI nr
Match: XP_022959549.1 (uncharacterized protein LOC111460588 [Cucurbita moschata])

HSP 1 Score: 2154.0 bits (5580), Expect = 0.0e+00
Identity = 1067/1078 (98.98%), Postives = 1068/1078 (99.07%), Query Frame = 0

Query: 9    MAFLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTD 68
            MAFLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTD
Sbjct: 1    MAFLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTD 60

Query: 69   RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL 128
            RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL
Sbjct: 61   RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL 120

Query: 129  SRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGL 188
            SRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGL
Sbjct: 121  SRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGL 180

Query: 189  TKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWGIRKAL 248
            TKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWGIRKAL
Sbjct: 181  TKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWGIRKAL 240

Query: 249  KLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSN 308
            KLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSN
Sbjct: 241  KLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSN 300

Query: 309  TEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLK 368
            TEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLK
Sbjct: 301  TEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLK 360

Query: 369  FIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTV 428
            FIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTV
Sbjct: 361  FIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTV 420

Query: 429  YKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYE 488
            YKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYE
Sbjct: 421  YKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYE 480

Query: 489  FVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLD 548
            FVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLD
Sbjct: 481  FVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLD 540

Query: 549  DNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLEL 608
            DNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLEL
Sbjct: 541  DNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLEL 600

Query: 609  ITGKKAVRFDGPEEDRNLAMYVLY----------VDECTYPWLNDCEHKNKCSNTEGNYT 668
            ITGKKAVRFDGPEEDRNLAMYVL+          VDECTYPWLNDCEHKNKCSNTEGNYT
Sbjct: 601  ITGKKAVRFDGPEEDRNLAMYVLFLLGLFEPFLDVDECTYPWLNDCEHKNKCSNTEGNYT 660

Query: 669  CHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQ 728
            CHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQ
Sbjct: 661  CHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQ 720

Query: 729  RFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLD 788
            RFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLD
Sbjct: 721  RFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLD 780

Query: 789  DGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGT 848
            DGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGT
Sbjct: 781  DGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGT 840

Query: 849  LFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAK 908
            LFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAK
Sbjct: 841  LFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAK 900

Query: 909  VSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKA 968
            VSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKA
Sbjct: 901  VSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKA 960

Query: 969  VRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAKLARECVRIKGEER 1028
            VRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAKLARECVRIKGEER
Sbjct: 961  VRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAKLARECVRIKGEER 1020

Query: 1029 PSMKEVAMELEALRATKGEHSWANVRLSAAQESSPFVEGGSMGAVDDSIKLELPHVEH 1077
            PSMKEVAMELEALRATKGEHSWANVRLSAAQESSPFVEGGSMGAVDDSIKLELPHVEH
Sbjct: 1021 PSMKEVAMELEALRATKGEHSWANVRLSAAQESSPFVEGGSMGAVDDSIKLELPHVEH 1078

BLAST of CmoCh04G025150 vs. NCBI nr
Match: XP_023542638.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111802455 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1675.2 bits (4337), Expect = 0.0e+00
Identity = 920/1487 (61.87%), Postives = 969/1487 (65.16%), Query Frame = 0

Query: 5    MKPIMAFLVM-MNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSIT 64
            MK IMAF+VM M IAI S  LA+ AS+ALPGC+EQCGDV IPYPFGIKE CYLNQNFSIT
Sbjct: 1    MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 65   CHKTDRNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVP 124
            C+KT                          +LHVL+P+VR CY   +      E++LS+P
Sbjct: 61   CNKT----------------------XIAMDLHVLQPVVRTCYDQVNGSFVPKESNLSLP 120

Query: 125  TMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLD 184
             M+ +S +KN+F+TIGCN + +I G+ QG+   SGC+SVC  +SSVV+G CSG+GCCQL+
Sbjct: 121  AMFSISGTKNRFVTIGCNTLSVIAGNHQGSLYGSGCLSVCLNKSSVVNGPCSGNGCCQLE 180

Query: 185  IPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWG 244
             P GLT LSLAV   LN+T++R F+PCGYAF+IEA RF+FLS YI+ FEE EVE VL WG
Sbjct: 181  FPNGLTNLSLAVSGFLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEE-EVEVVLDWG 240

Query: 245  IRKALKLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHK 304
            IR   + +CG N  R +SI +GT YRC CLDGY+GNPYL  GCQDV+EC   WLNDC HK
Sbjct: 241  IRNETRFECGSNTTR-SSILDGTEYRCQCLDGYDGNPYLPQGCQDVNECKSGWLNDCMHK 300

Query: 305  NKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLC 364
             KC NTEGNYTC CPKNFHGDGR+GG+GCTKNSTSSIPIIIGIGLGL VLLIA TTIYLC
Sbjct: 301  AKCINTEGNYTCRCPKNFHGDGREGGEGCTKNSTSSIPIIIGIGLGLVVLLIAITTIYLC 360

Query: 365  YKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKG 424
            YKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAG G
Sbjct: 361  YKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGNG 420

Query: 425  GYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVP 484
            GYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVP
Sbjct: 421  GYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVP 480

Query: 485  LLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTT 544
            LLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTT
Sbjct: 481  LLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTT 540

Query: 545  NVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGI 604
            N+LLDDNYTAKVSDFGASKLVP D TQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSF I
Sbjct: 541  NILLDDNYTAKVSDFGASKLVPMDQTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFEI 600

Query: 605  VLLELITGKKAVRFDGPEEDRNLAMYVLY------------------------------- 664
            VLLELITGKKAVRFDGPEEDRNLAMYVL                                
Sbjct: 601  VLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEERLEEVVEKGMVSEANMEQIKEVAKVA 660

Query: 665  ------------------------------------------------------------ 724
                                                                        
Sbjct: 661  RECVRIKGEERPSMKEVAMELEGLRATKGEHSWANVNLSSAEETVYLLDGASESSQLVGS 720

Query: 725  ------------------------------------------------------------ 784
                                                                        
Sbjct: 721  GSMGTVGNSIKLEITLIEHGRYLYNFYTPTTPICPLMVMKIKPIMTFLVMMNIAILSTPL 780

Query: 785  ------------------------------------------------------------ 844
                                                                        
Sbjct: 781  VVEASEALPGCDEWCGKVHIPYPFGIKKGCYLNQNFSITCHKTGPPKAEAFLMHTNIRVM 840

Query: 845  ------------------------------------------------------------ 904
                                                                        
Sbjct: 841  NISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPLSRSKNKFITIGCNHVGLIL 900

Query: 905  ------------------------------------------------------------ 964
                                                                        
Sbjct: 901  GDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGLTNLSLAVGELLNYTEIRKF 960

Query: 965  ------------------------------------------------------------ 1024
                                                                        
Sbjct: 961  SPCGYAFIIEAKRFQFLSSYIDKFEEKEVEVVLSWGIRKELELDCGLNARRNNSIFSETH 1020

Query: 1025 ---------------------VDECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRK 1079
                                 VDECTYPWLNDCEHK+KCSNTEGNYTCHCPKNFHGDGRK
Sbjct: 1021 YRCQCLDGYQGNPYLPQGCQDVDECTYPWLNDCEHKDKCSNTEGNYTCHCPKNFHGDGRK 1080

BLAST of CmoCh04G025150 vs. NCBI nr
Match: XP_022959563.1 (uncharacterized protein LOC111460594 [Cucurbita moschata])

HSP 1 Score: 1645.9 bits (4261), Expect = 0.0e+00
Identity = 943/1729 (54.54%), Postives = 1001/1729 (57.89%), Query Frame = 0

Query: 5    MKPIMAFLVM-MNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSIT 64
            MK IMAF+VM M IAI S  LA+ ASQALP C+++CGDVHIPYPFGIKE CYLNQNFSIT
Sbjct: 1    MKHIMAFMVMIMEIAILSTALAMVASQALPQCDDRCGDVHIPYPFGIKEGCYLNQNFSIT 60

Query: 65   CHKTDRNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVP 124
            C+KTDRN PPK  A+LM TNI V NISTNGELHVL+P+VR CY   +     NE++LSVP
Sbjct: 61   CNKTDRNGPPK--AYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPNESNLSVP 120

Query: 125  TMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLD 184
             M+ +S +KNKF+TIGCN + +I G+ QG+   SGCISVC  +SSVV+G CSG+GCCQL+
Sbjct: 121  AMFSISGTKNKFVTIGCNTLSVITGNHQGSLYGSGCISVCLNKSSVVNGPCSGNGCCQLE 180

Query: 185  IPKGLTKLSLAVGELLNYTEIRKFSPCGYAFII-EARRFQFLSSYIDKFEEKEVEAVLSW 244
             P GLT LS+AVG LLN+T++R F+PCGYAF+I E +RF+FLSSYI+ FE++EVE VL W
Sbjct: 181  FPNGLTNLSVAVGGLLNFTDVRTFNPCGYAFVIEEEKRFEFLSSYIENFEDEEVEVVLDW 240

Query: 245  GIRKALKLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEH 304
            GIR   + +CG N  R +SI +GT YRC CLDGY+GNPYL  GCQDVDEC Y WLNDCE+
Sbjct: 241  GIRNETRFECGSNTTR-SSILDGTEYRCQCLDGYDGNPYLPQGCQDVDECEYAWLNDCEY 300

Query: 305  KNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYL 364
            K+KC+NT+GNYTCHCPKNFHGDGR+GG+GCTKNSTSSIPIIIGIG+GL VLLIATTTIYL
Sbjct: 301  KDKCANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIIIGIGVGLPVLLIATTTIYL 360

Query: 365  CYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGK 424
             YKKLKFIKQKQ FFHKNGGFVLQRQLSQ NSP D+VRIFSQEELEKATNNYA+DTIAGK
Sbjct: 361  GYKKLKFIKQKQLFFHKNGGFVLQRQLSQWNSPNDMVRIFSQEELEKATNNYAQDTIAGK 420

Query: 425  GGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQV 484
            GG+GTVYKGVLDDGLT+AIKKSKFMDESQTSQFINEV+VLSQINHRNVVKLLGCCLETQV
Sbjct: 421  GGFGTVYKGVLDDGLTIAIKKSKFMDESQTSQFINEVIVLSQINHRNVVKLLGCCLETQV 480

Query: 485  PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKT 544
            PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKT
Sbjct: 481  PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKT 540

Query: 545  TNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFG 604
            TN+LLDDNYTAKVSDFGASKLVP D TQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFG
Sbjct: 541  TNILLDDNYTAKVSDFGASKLVPMDQTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFG 600

Query: 605  IVLLELITGKKAVRFDGPEEDRNLAMYVLY------------------------------ 664
            IVLLELITGKKAVRFDGPEEDRNLAMYVL                               
Sbjct: 601  IVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEERLEEVVEKGMVSEANMEQIKEAAKL 660

Query: 665  ------------------------------------------------------------ 724
                                                                        
Sbjct: 661  ARECVRIKGEERPSMKEVAMELEGLRATKGEHSWANVNLSSAEETVYLLDGASESSQLVG 720

Query: 725  ------------------------------------------------------------ 784
                                                                        
Sbjct: 721  SGSVGTVGNSIKLEITLMEHGRRVVQNCYRDAFTRVSNLSVPDMYPHSRSKNKFITIGCN 780

Query: 785  ------------------------------------------------------------ 844
                                                                        
Sbjct: 781  HVGLILGAYQGSDFESRCISMCTKNSSVVDESCSGSGCCQLEIPDGLSNLRLAVGELLHS 840

Query: 845  ------------------------------------------------------------ 904
                                                                        
Sbjct: 841  TDVWKFNPCGYAFIIEAERFKFLSSFIKNFKDKEVEVVLGWGIRNELELDCGLNARRNSS 900

Query: 905  ------------------------------------------------------------ 964
                                                                        
Sbjct: 901  IFNGTHYRCQCLDGYEGNPYLERMSRCLQATDQGRGAFESCAVTEGFESCAVTEGIVQCQ 960

Query: 965  ------------------------------------------------------------ 1024
                                                                        
Sbjct: 961  SIFRCRSLFWNAHTPTFCDELFNIFTIYTHQQQPCPLMVMNIKPIMAFMVMMMKIAILST 1020

Query: 1025 ------------------------------------------------------------ 1079
                                                                        
Sbjct: 1021 ALAVAASQALPGCDEWCGKVHIPYPFGIKKGCYLNQNFSITCHKTDRNRPPKALLMHTNI 1080

BLAST of CmoCh04G025150 vs. NCBI nr
Match: XP_022989772.1 (wall-associated receptor kinase 3-like [Cucurbita maxima])

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 687/1079 (63.67%), Postives = 708/1079 (65.62%), Query Frame = 0

Query: 1    MVMKMKPIMAFLV-MMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQN 60
            MVM +KPIMAF+V MM I I S  LAV AS+ALPGC+E CG VHIPYPFGIK+ CYLNQN
Sbjct: 1    MVMNIKPIMAFMVMMMKIGILSTALAVEASEALPGCDEWCGKVHIPYPFGIKKGCYLNQN 60

Query: 61   FSITCHKTDRNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETD 120
            FSITCHKTD N PPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETD
Sbjct: 61   FSITCHKTDLNGPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETD 120

Query: 121  LSVPTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGC 180
            LSVPTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRR SVVDGLCSGSGC
Sbjct: 121  LSVPTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRISVVDGLCSGSGC 180

Query: 181  CQLDIPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAV 240
            CQL+IPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVE V
Sbjct: 181  CQLEIPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEVV 240

Query: 241  LSWGIRKALKLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLND 300
            LSWGIRK L+LDCGLNARRN+SIFNGTHYRC CLDGYEGNPYL HGCQD           
Sbjct: 241  LSWGIRKELELDCGLNARRNSSIFNGTHYRCQCLDGYEGNPYLRHGCQD----------- 300

Query: 301  CEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTT 360
                                                                        
Sbjct: 301  ------------------------------------------------------------ 360

Query: 361  IYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTI 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  AGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLE 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  TQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRD 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  IKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVY 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  SFGIVLLELITGKKAVRFDGPEEDRNLAMYVLYVDECTYPWLNDCEHKNKCSNTEGNYTC 660
                                             VDEC YPWLNDC+HK+KCSNTEGNYTC
Sbjct: 601  ---------------------------------VDECKYPWLNDCKHKDKCSNTEGNYTC 660

Query: 661  HCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQR 720
            HCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIG+GL VLLIATTTIYL YKKLKFIKQKQ 
Sbjct: 661  HCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGVGLPVLLIATTTIYLGYKKLKFIKQKQL 720

Query: 721  FFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDD 780
            FF KNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYA+DTIAGKGGYGTV+KGVLDD
Sbjct: 721  FFQKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAQDTIAGKGGYGTVFKGVLDD 735

Query: 781  GLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTL 840
            GLT+AIKKSKFMDESQTSQFINEV+VLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTL
Sbjct: 781  GLTIAIKKSKFMDESQTSQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTL 735

Query: 841  FDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAKV 900
            FDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTN+LLD+NYTAKV
Sbjct: 841  FDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNILLDNNYTAKV 735

Query: 901  SDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAV 960
            SDFGASKLVPKD TQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIV+LELITGKKAV
Sbjct: 901  SDFGASKLVPKDQTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVVLELITGKKAV 735

Query: 961  RFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEAAKLARECVRIKGEERP 1020
            RFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKE AK+ARECVRIKGEERP
Sbjct: 961  RFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMVSEANMEQIKEVAKVARECVRIKGEERP 735

Query: 1021 SMKEVAMELEALRATKGEHSWANVRLSAAQESSPFVEGGSMGAVDDSIKLELPHVEHGR 1079
            SMKEVAMELEALRATKGEHSWANVRLSAA ESSPFVE GS+GAVDDS+KLELPHVEHGR
Sbjct: 1021 SMKEVAMELEALRATKGEHSWANVRLSAAGESSPFVESGSIGAVDDSMKLELPHVEHGR 735

BLAST of CmoCh04G025150 vs. NCBI nr
Match: XP_022960687.1 (wall-associated receptor kinase 3-like [Cucurbita moschata])

HSP 1 Score: 1241.1 bits (3210), Expect = 0.0e+00
Identity = 706/1449 (48.72%), Postives = 837/1449 (57.76%), Query Frame = 0

Query: 16   NIAIFS----APLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTDRNR 75
            NI I S    A  AVAA QALPGC+++CGD+ IPYPFG +E CYLN+NF ITC+ T  +R
Sbjct: 4    NILILSPLVYAATAVAAFQALPGCDDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHDDR 63

Query: 76   PPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTM-YPLSR 135
            P     FL   NI+V NIS +GELH+   + ++CY    N L    +  ++  + + +S 
Sbjct: 64   P---TPFLRTGNIQVTNISISGELHISNVVAKDCYP-KSNSLKARPSSATLNLLEFTVSS 123

Query: 136  SKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGLTK 195
            +KNKF  IGC+    + G  +G    +GC+++C   ++V DG CSG+GCCQLDIP GL  
Sbjct: 124  TKNKFTVIGCDTYAYLSGLIEGQTYTTGCMALCDNITTVRDGACSGNGCCQLDIPSGLKG 183

Query: 196  LSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEAVLSWGIRKALKL 255
            L   V    N++++  ++PCGYAF+ E  +F F  +YI  F +K V AVL WGI     L
Sbjct: 184  LRCRVQSFKNHSKVLSYNPCGYAFVTEEDKFNFSGAYIRNFTQKRVPAVLDWGISNTTCL 243

Query: 256  D--------CGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEH 315
                     CG N+ +  S+ +G+ YRC CL+G+EGNPYL  GCQD+DEC    LNDC  
Sbjct: 244  TANNESNCVCGPNSMKVTSLPDGSEYRCGCLEGFEGNPYLPQGCQDIDECRDERLNDC-- 303

Query: 316  KNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYL 375
            K +C NTEGNYTC+CP+ F GDGR+ G+GCT++S S + +IIG+ +G  VL+I +T +YL
Sbjct: 304  KFECVNTEGNYTCNCPEGFKGDGRRRGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYL 363

Query: 376  CYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGK 435
             Y+K K IK K++FF ++GG +LQR LSQ  S  D+V IF+QEEL+KATN Y +  + GK
Sbjct: 364  GYRKWKLIKLKEKFFEEHGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGK 423

Query: 436  GGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQV 495
            GGYGTVYKG+L DG  VAIKKSK +D+SQTSQFINEV+VLSQINHRNVV+L+GCCLETQV
Sbjct: 424  GGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVRLVGCCLETQV 483

Query: 496  PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKT 555
            PLLVYEF+TNGTLFDHIHDTTKHVPLSW+ARLRIASETAGVISYLHSSASIPIIHRDIKT
Sbjct: 484  PLLVYEFITNGTLFDHIHDTTKHVPLSWKARLRIASETAGVISYLHSSASIPIIHRDIKT 543

Query: 556  TNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFG 615
            TN+LLDDNY AKVSDFGASKLVP D TQL+T+VQGT GYLDPEYLLTSELTEKSDVYSFG
Sbjct: 544  TNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTFGYLDPEYLLTSELTEKSDVYSFG 603

Query: 616  IVLLELITGKKAVRFDGPEEDRNLAMYVLY------------------------------ 675
            IVLLELITGKKA  F+GPE +RNLA+YVL                               
Sbjct: 604  IVLLELITGKKAGNFEGPEAERNLAVYVLRAMKEDRLGDVVEKGMAREREFEQIKEVGKV 663

Query: 676  ------------------------------------------------------------ 735
                                                                        
Sbjct: 664  ARKCLRISGEERPSMKEVGMELEGLRVMVEHEWVDEEKLVADGALDSTSNQFRQIETLIR 723

Query: 736  ------------------------------------------------------------ 795
                                                                        
Sbjct: 724  LMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHFNPPR 783

Query: 796  ------------------------------------------------------------ 855
                                                                        
Sbjct: 784  PFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTVSSTKNKFT 843

Query: 856  ------------------------------------------------------------ 915
                                                                        
Sbjct: 844  VIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLRYRVR 903

Query: 916  ------------------------------------------------------------ 975
                                                                        
Sbjct: 904  SFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTTCSTANNKS 963

Query: 976  ----------------------------------------VDECTYPWLNDCEHKNKCSN 1035
                                                    +DEC    LNDC  K +C N
Sbjct: 964  NCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDERLNDC--KFECVN 1023

Query: 1036 TEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLK 1079
            T+GNYTC+CP+ F GDGR+GG+GCT++S S + +IIG+ +G  VL+I +T +YL Y+K K
Sbjct: 1024 TKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYRKWK 1083

BLAST of CmoCh04G025150 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 481.1 bits (1237), Expect = 2.3e-135
Identity = 264/608 (43.42%), Postives = 371/608 (61.02%), Query Frame = 0

Query: 35  CEEQCGDVHIPYPFGIKERCYL--NQNFSITCHKTDRNRPPKAEAFLMHTNIRVMNISTN 94
           C+ +CG+V + YPFG    CY   +++F++TC++ ++         L   N+ V+N+S +
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQEK---------LFFGNMPVINMSLS 88

Query: 95  GELHVLRPIVRNCYSYGDNYLD--MNETDLSVPTMYPLSRSKNKFITIGCNHVGLILGDD 154
           G+L V     R CY       D     T L   T+  L    N+F  +GCN     L   
Sbjct: 89  GQLRVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL----NRFTVVGCNSYA-FLRTS 148

Query: 155 QGTNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPKGLTKLSLAVGELLNYTEIRKFSPC 214
                 +GCIS+C   ++  +G CSG GCCQ+ +P+G + + +      N+  +  F+PC
Sbjct: 149 GVEKYSTGCISICD-SATTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPC 208

Query: 215 GYAFIIEARRFQF--LSSYIDKFEEKEVEAVLSWGI------RKALKLDCGLNARRNNSI 274
            YAF++E   F F  L    +         VL W I      +   +  CG N+   +S 
Sbjct: 209 TYAFLVEDGMFDFHALEDLNNLRNVTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDST 268

Query: 275 FNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFH 334
             GT Y C CL+G+EGNPYL +GCQD++EC     N  EH + C NT+G++ C+CP  + 
Sbjct: 269 -GGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEH-STCENTKGSFNCNCPSGYR 328

Query: 335 GDGRKGGKGCTKNSTSS----IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFH 394
            D       CT+           I +G  +G +V+++  + +    K  K  + +Q+FF 
Sbjct: 329 KDSL---NSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQKFFE 388

Query: 395 KNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDDGLT 454
           +NGG +L +++S        V+IF+++ +++ATN Y +  I G+GG GTVYKG+L D   
Sbjct: 389 QNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPDNSI 448

Query: 455 VAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTLFDH 514
           VAIKK++  + SQ  QFINEV+VLSQINHRNVVK+LGCCLET+VPLLVYEF+ +GTLFDH
Sbjct: 449 VAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTLFDH 508

Query: 515 IHDTTKHVPLSWEARLRIASETAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAKVSDF 574
           +H +     L+WE RLRIA+E AG ++YLHSSASIPIIHRDIKT N+LLD N TAKV+DF
Sbjct: 509 LHGSLYDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKVADF 568

Query: 575 GASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAVRFD 627
           GAS+L+P D  QLTT+VQGTLGYLDPEY  T  L EKSDVYSFG+VL+EL++G+KA+ F+
Sbjct: 569 GASRLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCFE 616

BLAST of CmoCh04G025150 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 475.7 bits (1223), Expect = 9.8e-134
Identity = 278/636 (43.71%), Postives = 374/636 (58.81%), Query Frame = 0

Query: 11  FLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYL--NQNFSITCHKTD 70
           FLV +  ++    L     Q    C+ +CG++ I YPFGI   CY   N++FSITC K D
Sbjct: 8   FLVAIFFSLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITC-KED 67

Query: 71  RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL 130
           R          + ++I V N + +G+L VL      CY   D      E D S  T+  L
Sbjct: 68  RPH--------VLSDIEVANFNHSGQLQVLLNRSSTCY---DEQGKKTEEDSSF-TLENL 127

Query: 131 SRS-KNKFITIGCNHVGLILGDDQG-TNVESGCISVCTRRSSVVDGLCSGSGCCQLDIPK 190
           S S  NK   +GCN + L+  D  G  N  + C+S+C       DG C+G GCC++D+  
Sbjct: 128 SLSANNKLTAVGCNALSLL--DTFGMQNYSTACLSLCDSPPE-ADGECNGRGCCRVDVSA 187

Query: 191 GLTKLSLAV--GELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEV---EAVLS 250
            L   +     G + + T    FSPC YAF++E  +F F SS  D    + V     +L 
Sbjct: 188 PLDSYTFETTSGRIKHMTSFHDFSPCTYAFLVEDDKFNF-SSTEDLLNLRNVMRFPVLLD 247

Query: 251 WGIRKALKLDCGLNA--RRNNSIFNGT---HYRCHCLDGYEGNPYLHHGCQDVDECTYP- 310
           W +        G  +    N++  + T    Y C C +G++GNPYL  GCQDV+ECT   
Sbjct: 248 WSVGNQTCEQVGSTSICGGNSTCLDSTPRNGYICRCNEGFDGNPYLSAGCQDVNECTTSS 307

Query: 311 --WLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLGLAVL 370
               ++C     C N  G + C C   +  D       C +   +   I++   +G  V+
Sbjct: 308 TIHRHNCSDPKTCRNKVGGFYCKCQSGYRLDTTT--MSCKRKEFAWTTILLVTTIGFLVI 367

Query: 371 LIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNN 430
           L+    I    K LK  K +++FF +NGG +L ++LS        V+IF+++ ++KATN 
Sbjct: 368 LLGVACIQQRMKHLKDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNG 427

Query: 431 YAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKL 490
           YA+  I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV+VLSQINHRNVVKL
Sbjct: 428 YAESRILGQGGQGTVYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKL 487

Query: 491 LGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASI 550
           LGCCLET+VPLLVYEF+TNGTLFDH+H +     L+WE RL+IA E AG ++YLHSSASI
Sbjct: 488 LGCCLETEVPLLVYEFITNGTLFDHLHGSMIDSSLTWEHRLKIAIEVAGTLAYLHSSASI 547

Query: 551 PIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELT 610
           PIIHRDIKT N+LLD N TAKV+DFGAS+L+P D  +L T+VQGTLGYLDPEY  T  L 
Sbjct: 548 PIIHRDIKTANILLDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLN 607

Query: 611 EKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMY 630
           EKSDVYSFG+VL+EL++G+KA+ F  P+  ++L  Y
Sbjct: 608 EKSDVYSFGVVLMELLSGQKALCFKRPQSSKHLVSY 624

HSP 2 Score: 416.0 bits (1068), Expect = 9.2e-116
Identity = 214/413 (51.82%), Postives = 286/413 (69.25%), Query Frame = 0

Query: 633  VDECTYP---WLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIG 692
            V+ECT       ++C     C N  G + C C   +  D       C +   +   I++ 
Sbjct: 283  VNECTTSSTIHRHNCSDPKTCRNKVGGFYCKCQSGYRLDTTT--MSCKRKEFAWTTILLV 342

Query: 693  IGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQE 752
              +G  V+L+    I    K LK  K +++FF +NGG +L ++LS        V+IF+++
Sbjct: 343  TTIGFLVILLGVACIQQRMKHLKDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTED 402

Query: 753  ELEKATNNYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQI 812
             ++KATN YA+  I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV+VLSQI
Sbjct: 403  GMKKATNGYAESRILGQGGQGTVYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQI 462

Query: 813  NHRNVVKLLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVIS 872
            NHRNVVKLLGCCLET+VPLLVYEF+TNGTLFDH+H +     L+WE RL+IA E AG ++
Sbjct: 463  NHRNVVKLLGCCLETEVPLLVYEFITNGTLFDHLHGSMIDSSLTWEHRLKIAIEVAGTLA 522

Query: 873  YLHSSASIPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPE 932
            YLHSSASIPIIHRDIKT N+LLD N TAKV+DFGAS+L+P D  +L T+VQGTLGYLDPE
Sbjct: 523  YLHSSASIPIIHRDIKTANILLDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPE 582

Query: 933  YLLTSELTEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEDRFEEVVEK 992
            Y  T  L EKSDVYSFG+VL+EL++G+KA+ F  P+  ++L  Y   A KE+R +E++  
Sbjct: 583  YYNTGLLNEKSDVYSFGVVLMELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGG 642

Query: 993  GMVSEANMEQIKEAAKLARECVRIKGEERPSMKEVAMELEALRATKGEHSWAN 1043
             +++E N+++I+EAA++A EC R+ GEERP MKEVA +LEALR  K +H W++
Sbjct: 643  EVMNEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSD 693

BLAST of CmoCh04G025150 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 475.3 bits (1222), Expect = 1.3e-133
Identity = 277/646 (42.88%), Postives = 374/646 (57.89%), Query Frame = 0

Query: 11  FLVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYL--NQNFSITCHKTD 70
           FLV++    ++  L     Q    C+ +CG+V I YPFGI   CY   + NF++TC    
Sbjct: 8   FLVVIFFLAYT-QLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC---- 67

Query: 71  RNRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPL 130
                  E  L+   I+V NIS +G + VL      CY    N  +       + + + L
Sbjct: 68  ----VVEEKLLLFGIIQVTNISHSGHVSVLFERFSECYEQ-KNETNGTALGYQLGSSFSL 127

Query: 131 SRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLD----- 190
           S S NKF  +GCN + L L      N  +GC+S+C  +    +G C+G GCC  +     
Sbjct: 128 S-SNNKFTLVGCNALSL-LSTFGKQNYSTGCLSLCNSQPE-ANGRCNGVGCCTTEDFSVP 187

Query: 191 -----IPKGLTKLSLAVGELLNY--TEIRKFSPCGYAFIIEARRFQFLSSYIDK--FEEK 250
                   G  +L   V   L+   T + +F+PC YAF++E  +F F SS   K      
Sbjct: 188 FDSDTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVT 247

Query: 251 EVEAVLSWGIRKALKLDCGLN--ARRNNSIFNGT---HYRCHCLDGYEGNPYLHHGCQDV 310
                L W I        G      +N+S +N T    Y C C +GY+GNPY   GC+D+
Sbjct: 248 RFPVALDWSIGNQTCEQAGSTRICGKNSSCYNSTTRNGYICKCNEGYDGNPYRSEGCKDI 307

Query: 311 DECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGLG 370
           DEC     ++C     C N +G + C CP    G        CT+       I + I +G
Sbjct: 308 DECISD-THNCSDPKTCRNRDGGFDCKCPS---GYDLNSSMSCTRPEYKRTRIFLVIIIG 367

Query: 371 LAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEK 430
           + VLL+A   I    K+ K+ K +++FF +NGG +L ++LS         +IF++E +++
Sbjct: 368 VLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKE 427

Query: 431 ATNNYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRN 490
           ATN Y +  I G+GG GTVYKG+L D   VAIKK++  D  Q  QFI+EV+VLSQINHRN
Sbjct: 428 ATNGYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRN 487

Query: 491 VVKLLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHS 550
           VVK+LGCCLET+VPLLVYEF+TNGTLFDH+H +     L+WE RLRIA E AG ++YLHS
Sbjct: 488 VVKILGCCLETEVPLLVYEFITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYLHS 547

Query: 551 SASIPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLT 610
           SASIPIIHRDIKT N+LLD+N TAKV+DFGASKL+P D  QLTT+VQGTLGYLDPEY  T
Sbjct: 548 SASIPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYTT 607

Query: 611 SELTEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMYVLYVDE 636
             L EKSDVYSFG+VL+EL++G+KA+ F+ P+  ++L  Y +   E
Sbjct: 608 GLLNEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATE 636

HSP 2 Score: 426.0 bits (1094), Expect = 8.9e-119
Identity = 215/410 (52.44%), Postives = 290/410 (70.73%), Query Frame = 0

Query: 633  VDECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIIIGIGL 692
            +DEC     ++C     C N +G + C CP    G        CT+       I + I +
Sbjct: 294  IDECISD-THNCSDPKTCRNRDGGFDCKCPS---GYDLNSSMSCTRPEYKRTRIFLVIII 353

Query: 693  GLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELE 752
            G+ VLL+A   I    K+ K+ K +++FF +NGG +L ++LS         +IF++E ++
Sbjct: 354  GVLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMK 413

Query: 753  KATNNYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHR 812
            +ATN Y +  I G+GG GTVYKG+L D   VAIKK++  D  Q  QFI+EV+VLSQINHR
Sbjct: 414  EATNGYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHR 473

Query: 813  NVVKLLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLH 872
            NVVK+LGCCLET+VPLLVYEF+TNGTLFDH+H +     L+WE RLRIA E AG ++YLH
Sbjct: 474  NVVKILGCCLETEVPLLVYEFITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYLH 533

Query: 873  SSASIPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLL 932
            SSASIPIIHRDIKT N+LLD+N TAKV+DFGASKL+P D  QLTT+VQGTLGYLDPEY  
Sbjct: 534  SSASIPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYT 593

Query: 933  TSELTEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEDRFEEVVEKGMV 992
            T  L EKSDVYSFG+VL+EL++G+KA+ F+ P+  ++L  Y + A +E+R  E+++  ++
Sbjct: 594  TGLLNEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQVL 653

Query: 993  SEANMEQIKEAAKLARECVRIKGEERPSMKEVAMELEALRATKGEHSWAN 1043
            +E N+++I+EAA++A EC R+ GEERP MKEVA +LEALR  K +H W++
Sbjct: 654  NEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSD 699

BLAST of CmoCh04G025150 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 467.6 bits (1202), Expect = 2.7e-131
Identity = 268/636 (42.14%), Postives = 369/636 (58.02%), Query Frame = 0

Query: 12  LVMMNIAIFSAPLAVAASQALPGCEEQCGDVHIPYPFGIKERCYL--NQNFSITCHKTDR 71
           L +M I  + A   +  +Q    C+ +CGDV I YPFGI   CY   + +F+ITC +   
Sbjct: 6   LFLMAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEEDKP 65

Query: 72  NRPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPLS 131
           N         + +NI V+N + +G+L  L P    CY    N    N+ +     +  LS
Sbjct: 66  N---------VLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTN----NDFESLWFRLDNLS 125

Query: 132 RS-KNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCC--QLDIPK 191
            S  NKF  +GCN    +L      N  +GC+S+C       +  C+G GCC  ++ IP 
Sbjct: 126 FSPNNKFTLVGCN-AWALLSTFGIQNYSTGCMSLCD-TPPPPNSKCNGVGCCRTEVSIPL 185

Query: 192 GLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQF--LSSYIDKFEEKEVEAVLSWGI 251
              ++        N T +  F+PC YAF +E   F F  L    D         +L W I
Sbjct: 186 DSHRIETQPSRFENMTSVEHFNPCSYAFFVEDGMFNFSSLEDLKDLRNVTRFPVLLDWSI 245

Query: 252 -RKALKLDCGLNARRNNSI----FNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYPWLND 311
             +  +   G N    NS       G  Y C CL G++GNPYL  GCQD++ECT   +++
Sbjct: 246 GNQTCEQVVGRNICGGNSTCFDSTRGKGYNCKCLQGFDGNPYLSDGCQDINECT-TRIHN 305

Query: 312 CEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSS------IPIIIGIGLGLAVL 371
           C   + C NT G++ C CP     D       C               +++G  +G  ++
Sbjct: 306 CSDTSTCENTLGSFHCQCPSG--SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLII 365

Query: 372 LIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATNN 431
           L+  + I    +  K  + +Q+FF +NGG +L ++LS        V+IF++E +++AT+ 
Sbjct: 366 LLTISYIQQKMRHRKNTELRQQFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDG 425

Query: 432 YAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVKL 491
           Y +  I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV+VLSQINHRNVVKL
Sbjct: 426 YNESRILGQGGQGTVYKGILQDNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKL 485

Query: 492 LGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASI 551
           LGCCLET+VPLLVYEF+++GTLFDH+H +     L+WE RLRIA E AG ++YLHS ASI
Sbjct: 486 LGCCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRIAIEVAGTLAYLHSYASI 545

Query: 552 PIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSELT 611
           PIIHRD+KT N+LLD+N TAKV+DFGAS+L+P D  QLTT+VQGTLGYLDPEY  T  L 
Sbjct: 546 PIIHRDVKTANILLDENLTAKVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLN 605

Query: 612 EKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMY 630
           EKSDVYSFG+VL+EL++G+KA+ F+ P+  ++L  Y
Sbjct: 606 EKSDVYSFGVVLMELLSGEKALCFERPQSSKHLVSY 623

BLAST of CmoCh04G025150 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 463.4 bits (1191), Expect = 5.0e-130
Identity = 268/637 (42.07%), Postives = 379/637 (59.50%), Query Frame = 0

Query: 17  IAIFS-APLAVAASQALPGCEEQCGDVHIPYPFGIKERCYLNQNFSITCHKTDRNRPPKA 76
           +AIF  + + +   Q LP C E+CG+V + YPFG    C+  ++ S      + N     
Sbjct: 9   VAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCVNEN----- 68

Query: 77  EAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPTMYPLSRSKNKF 136
              L +  + V+ IS + +L VL P    CY+    +    +       +  L+ S N  
Sbjct: 69  ---LFYKGLEVVEISHSSQLRVLYPASYICYNSKGKFA---KGTYYWSNLGNLTLSGNNT 128

Query: 137 IT-IGCNHVGLILGDDQGTNVES-GCISVCTRRSSVVDGLCSGSGCCQLDIPKGLTKLSL 196
           IT +GCN    +     GT   S GCIS C   S   +G C+G GCCQ  +P G   L +
Sbjct: 129 ITALGCNSYAFV--SSNGTRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIV 188

Query: 197 AVGELLNYTEIRKFS--PCGYAFIIEARRFQFLSSYIDKFEEKE-----VEAVLSWGIR- 256
                 N T ++  S   C YAF++E  +F++ +S  DK+   +        VL W IR 
Sbjct: 189 RSYRFDNDTSVQPISEGQCIYAFLVENGKFKYNAS--DKYSYLQNRNVGFPVVLDWSIRG 248

Query: 257 ----KALKLDCGLNARRNNSIFNGTHYRCHCLDGYEGNPYLHHGCQDVDECTYP---WLN 316
               +  +  CG+N   +NS  +G  Y C C  G++GNPYL +GCQD++ECT       +
Sbjct: 249 ETCGQVGEKKCGVNGICSNSA-SGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKH 308

Query: 317 DCEHKNKCSNTEGNYTCHCPKNFHGDGR------KGGKGCTKNSTSSIPIIIGIGLGLAV 376
           +C   + C N  G++ C+C   +  +        KG     + +T    I++G  +G  V
Sbjct: 309 NCSGDSTCENKLGHFRCNCRSRYELNTTTNTCKPKGNPEYVEWTT----IVLGTTIGFLV 368

Query: 377 LLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIVRIFSQEELEKATN 436
           +L+A + I    K  K  + +Q+FF +NGG +L ++LS        V+IF++E +++AT+
Sbjct: 369 ILLAISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATD 428

Query: 437 NYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEVVVLSQINHRNVVK 496
            Y ++ I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV+VLSQINHRNVVK
Sbjct: 429 GYDENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVK 488

Query: 497 LLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSAS 556
           LLGCCLET+VPLLVYEF+++GTLFDH+H +     L+WE RLR+A E AG ++YLHSSAS
Sbjct: 489 LLGCCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRMAVEIAGTLAYLHSSAS 548

Query: 557 IPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTLGYLDPEYLLTSEL 616
           IPIIHRDIKT N+LLD+N TAKV+DFGAS+L+P D   L T+VQGTLGYLDPEY  T  L
Sbjct: 549 IPIIHRDIKTANILLDENLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLL 608

Query: 617 TEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMY 630
            EKSDVYSFG+VL+EL++G+KA+ F+ P+  +++  Y
Sbjct: 609 NEKSDVYSFGVVLMELLSGQKALCFERPQTSKHIVSY 625

HSP 2 Score: 407.5 bits (1046), Expect = 3.3e-113
Identity = 211/419 (50.36%), Postives = 293/419 (69.93%), Query Frame = 0

Query: 633  VDECTYP---WLNDCEHKNKCSNTEGNYTCHCPKNFHGDGR------KGGKGCTKNSTSS 692
            ++ECT       ++C   + C N  G++ C+C   +  +        KG     + +T  
Sbjct: 280  INECTTANPIHKHNCSGDSTCENKLGHFRCNCRSRYELNTTTNTCKPKGNPEYVEWTT-- 339

Query: 693  IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIV 752
              I++G  +G  V+L+A + I    K  K  + +Q+FF +NGG +L ++LS        V
Sbjct: 340  --IVLGTTIGFLVILLAISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDV 399

Query: 753  RIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDDGLTVAIKKSKFMDESQTSQFINEV 812
            +IF++E +++AT+ Y ++ I G+GG GTVYKG+L D   VAIKK++  D SQ  QFINEV
Sbjct: 400  KIFTEEGMKEATDGYDENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEV 459

Query: 813  VVLSQINHRNVVKLLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASE 872
            +VLSQINHRNVVKLLGCCLET+VPLLVYEF+++GTLFDH+H +     L+WE RLR+A E
Sbjct: 460  LVLSQINHRNVVKLLGCCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRMAVE 519

Query: 873  TAGVISYLHSSASIPIIHRDIKTTNVLLDDNYTAKVSDFGASKLVPKDHTQLTTLVQGTL 932
             AG ++YLHSSASIPIIHRDIKT N+LLD+N TAKV+DFGAS+L+P D   L T+VQGTL
Sbjct: 520  IAGTLAYLHSSASIPIIHRDIKTANILLDENLTAKVADFGASRLIPMDKEDLATMVQGTL 579

Query: 933  GYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAVRFDGPEEDRNLAMYVLCAIKEDRF 992
            GYLDPEY  T  L EKSDVYSFG+VL+EL++G+KA+ F+ P+  +++  Y   A KE+R 
Sbjct: 580  GYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCFERPQTSKHIVSYFASATKENRL 639

Query: 993  EEVVEKGMVSEANMEQIKEAAKLARECVRIKGEERPSMKEVAMELEALRATKGEHSWAN 1043
             E+++  +++E N  +I++AA++A EC R+ GEERP MKEVA ELEALR TK +H W++
Sbjct: 640  HEIIDGQVMNENNQREIQKAARIAVECTRLTGEERPGMKEVAAELEALRVTKTKHKWSD 694