CmoCh04G023680 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G023680
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionCoA ligase
LocationCmo_Chr04: 17670676 .. 17673139 (-)
RNA-Seq ExpressionCmoCh04G023680
SyntenyCmoCh04G023680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCTATTTCTTCTCTCTCTAACAGAGAAAAAACTCTGAGCTTTTTTCTTTTTTTTTATTTTTAATTATTTTCGAAATTATGAATTCCCTTTCGAAATGCGATGCGAACTATGCGTCTCTTACGCCCATCACCTTCTTGAAGAGAGCGTCGGCGTTTTACGCCGATCGGACTTCCATTATCTATGAAGGAACTCGCTTCACATGGCGCCAGACTTACCAACGCTGCTGCCGCCTCGCCTCCTCGCTTTCCCGCCTCAACGTCGCTAAGAACACCGTTGTAAGACCTCTTTCTTCTCGTTTTTTGAAAAAATTGTTGGAAGTGTTAGACGAAGAACATCACGATTAATTAATCGTGTGATAGAGAGAGTATTATTTGATTATAAACTCATGATCATCTCCTAAATTAGTTGAGGTGAGACTTTCAAGCATAGTGACACGGTGCAAAATGGTCAATATCATACCATTGTACGTGATTTTAACAAAAACATCTCATTTTGATCCTTTCGAAATTTTTGAACATGGGTAGAAGTTTAAATTTATTAATAACCGTGGAGAGTCGTAATTCCTAATAAAAATGTCCCATTTTGATCTTCTCTAAATTTATGATCAGGGTTTGTATGTTATTGGGAGAGTATGTGATTTCTAATAAAAACGTCCCATTTTAATCTTTTTTTCAAATTTCTCAACATGGGTATGAGTTTAAATTTATTGACAATCTCAATTCTTGAAGGTGTCGGTGTTGGCGCCCAATGTTCCGGCATTGTACGAGATGCATTTTGCAGTACCGATGGCCGGTGCTATTCTGAACACAATCAACACCCGCCTCGACGTCAATAACATCGCGTTGATCCTCCGCCACTCCGAGGCGAAGATTTTGTTCGTTGACTACCAATTCATACAAGAAGCCAAAGATGCCCTCCGTCAATTGATGGCGGCGTCCCTCCCGCTTCCATTTGTTGTCGTCATCGACGACATCGACTCTCCCACCGGTGCCCGTTTCGGGGAATTGGAATATGAGCAACTCGTCCACGACGGCGATGTTGGGTTCACCCCAGTAGAGGTTGACGACGAATGGGATTCAATAGCTTTGAATTACACCTCCGGCACAACATCGGCGCCGAAGGGCGTGGTGTACAGTCACCGCGGCGCGTATCTCAGTACGCTTAGTCTGGTGATGGGATGGGAAATGGGTAACGCGCCTGTTTATTTGTGGACTTTGCCGATGTTCCATTGTAATGGATGGACGTTCACGTGGGGGATTGCAGCTCGTGGTGGGACTAATGTCTGCATGCGTAATACGACGGCGTTTGATATTTACCGGAATATTAATTTGCATCATGTGACTCACATGTGCTGTGCGCCGATTGTGTTCCGTATCATCCTTGAGGCTAACGGCGGTGAACGGCGGCGATTTGGATGGCTAGTTAATGTGCTGACTGGCGGAGCGCCGCCACCGGCGGCGCTCCTGGAGAAAATGGAGGCTCTTGGGTTTCATATTACTCATGCTTATGGGCTGACGGAGGCCACCGGACCGGCGTTGGTCTGCGAGTGGCAAGAAAAATGGAATATTCTGCCGGCCGATGAACAAGCCAAGCTTAAAGCTAGACAAGGTATTTTTTTTAATTAATTAATTTTTTTTTCATAAAAAATATATTTTTTATGAAAAAAACGTGAAATTTGAAAATTATAAAAAATACCCAAAATCGGGAAAATTAATTGATATTTATTTCACTATTTTGCGGATTAGGAATCAGCATTTTGACATTAGCTGACGTGGACGTCAAAAACTTGGAGACAATGGACAGCGTGCCACACGATGGTCGGACCACCGGCGAGATCGTCCTACGTGGCAGCAGTATCATGAAGGGCTACTTCAAGGATCCGGCAGCCACCGCAAAGGCCTTCCAAAATGGGTGGTTTCTCACCGGCGACGTCGGCGTGATACACCCAGATGGGTATTTGGAGATAAAGGACAGGTCGAAGGACGTCATCATCTCAGGCGGTGAGAACATCAGCAGCGTGGAGGTCGAGACGGTGCTGTACCGGCACCCACAGGTGGCGGAGGCAGCGGTGGTGGCAATGCCACACCCCCGGTGGGGTGAGAGCCCGTGCGCCTTCGTGGTGGTCCACAGCGACGGCGGCAAGGTGAGTGAGGCGGAGATGATTGAATTTTGCCGGAAGAATATGTCGCACTATATGGCGCCGAAGAAGGTGGTGTTCGTGGCGGAGCTTCCCAAGACGTTGACCGGAAAGATTCAGAAGTTCAAACTGAGGGAGCAGGCGAAATTGTTGGTTGGATCTGAGAAACGTGTCTCCGGCGGGAACAAGTCCGGGCGGCACGACGGTGGGCGGCAGCCGGATTACGGTCAGCATGTTATGGCCATGTCACGGCTTTGAGTGGGGCCGGTTTTGTTGATGTACAATTAAAAACATGTACTTGATGATGAAGAATGAGTGGTGGGTA

mRNA sequence

CTCCTATTTCTTCTCTCTCTAACAGAGAAAAAACTCTGAGCTTTTTTCTTTTTTTTTATTTTTAATTATTTTCGAAATTATGAATTCCCTTTCGAAATGCGATGCGAACTATGCGTCTCTTACGCCCATCACCTTCTTGAAGAGAGCGTCGGCGTTTTACGCCGATCGGACTTCCATTATCTATGAAGGAACTCGCTTCACATGGCGCCAGACTTACCAACGCTGCTGCCGCCTCGCCTCCTCGCTTTCCCGCCTCAACGTCGCTAAGAACACCGTTGTGTCGGTGTTGGCGCCCAATGTTCCGGCATTGTACGAGATGCATTTTGCAGTACCGATGGCCGGTGCTATTCTGAACACAATCAACACCCGCCTCGACGTCAATAACATCGCGTTGATCCTCCGCCACTCCGAGGCGAAGATTTTGTTCGTTGACTACCAATTCATACAAGAAGCCAAAGATGCCCTCCGTCAATTGATGGCGGCGTCCCTCCCGCTTCCATTTGTTGTCGTCATCGACGACATCGACTCTCCCACCGGTGCCCGTTTCGGGGAATTGGAATATGAGCAACTCGTCCACGACGGCGATGTTGGGTTCACCCCAGTAGAGGTTGACGACGAATGGGATTCAATAGCTTTGAATTACACCTCCGGCACAACATCGGCGCCGAAGGGCGTGGTGTACAGTCACCGCGGCGCGTATCTCAGTACGCTTAGTCTGGTGATGGGATGGGAAATGGGTAACGCGCCTGTTTATTTGTGGACTTTGCCGATGTTCCATTGTAATGGATGGACGTTCACGTGGGGGATTGCAGCTCGTGGTGGGACTAATGTCTGCATGCGTAATACGACGGCGTTTGATATTTACCGGAATATTAATTTGCATCATGTGACTCACATGTGCTGTGCGCCGATTGTGTTCCGTATCATCCTTGAGGCTAACGGCGGTGAACGGCGGCGATTTGGATGGCTAGTTAATGTGCTGACTGGCGGAGCGCCGCCACCGGCGGCGCTCCTGGAGAAAATGGAGGCTCTTGGGTTTCATATTACTCATGCTTATGGGCTGACGGAGGCCACCGGACCGGCGTTGGTCTGCGAGTGGCAAGAAAAATGGAATATTCTGCCGGCCGATGAACAAGCCAAGCTTAAAGCTAGACAAGGAATCAGCATTTTGACATTAGCTGACGTGGACGTCAAAAACTTGGAGACAATGGACAGCGTGCCACACGATGGTCGGACCACCGGCGAGATCGTCCTACGTGGCAGCAGTATCATGAAGGGCTACTTCAAGGATCCGGCAGCCACCGCAAAGGCCTTCCAAAATGGGTGGTTTCTCACCGGCGACGTCGGCGTGATACACCCAGATGGGTATTTGGAGATAAAGGACAGGTCGAAGGACGTCATCATCTCAGGCGGTGAGAACATCAGCAGCGTGGAGGTCGAGACGGTGCTGTACCGGCACCCACAGGTGGCGGAGGCAGCGGTGGTGGCAATGCCACACCCCCGGTGGGGTGAGAGCCCGTGCGCCTTCGTGGTGGTCCACAGCGACGGCGGCAAGGTGAGTGAGGCGGAGATGATTGAATTTTGCCGGAAGAATATGTCGCACTATATGGCGCCGAAGAAGGTGGTGTTCGTGGCGGAGCTTCCCAAGACGTTGACCGGAAAGATTCAGAAGTTCAAACTGAGGGAGCAGGCGAAATTGTTGGTTGGATCTGAGAAACGTGTCTCCGGCGGGAACAAGTCCGGGCGGCACGACGGTGGGCGGCAGCCGGATTACGGTCAGCATGTTATGGCCATGTCACGGCTTTGAGTGGGGCCGGTTTTGTTGATGTACAATTAAAAACATGTACTTGATGATGAAGAATGAGTGGTGGGTA

Coding sequence (CDS)

ATGAATTCCCTTTCGAAATGCGATGCGAACTATGCGTCTCTTACGCCCATCACCTTCTTGAAGAGAGCGTCGGCGTTTTACGCCGATCGGACTTCCATTATCTATGAAGGAACTCGCTTCACATGGCGCCAGACTTACCAACGCTGCTGCCGCCTCGCCTCCTCGCTTTCCCGCCTCAACGTCGCTAAGAACACCGTTGTGTCGGTGTTGGCGCCCAATGTTCCGGCATTGTACGAGATGCATTTTGCAGTACCGATGGCCGGTGCTATTCTGAACACAATCAACACCCGCCTCGACGTCAATAACATCGCGTTGATCCTCCGCCACTCCGAGGCGAAGATTTTGTTCGTTGACTACCAATTCATACAAGAAGCCAAAGATGCCCTCCGTCAATTGATGGCGGCGTCCCTCCCGCTTCCATTTGTTGTCGTCATCGACGACATCGACTCTCCCACCGGTGCCCGTTTCGGGGAATTGGAATATGAGCAACTCGTCCACGACGGCGATGTTGGGTTCACCCCAGTAGAGGTTGACGACGAATGGGATTCAATAGCTTTGAATTACACCTCCGGCACAACATCGGCGCCGAAGGGCGTGGTGTACAGTCACCGCGGCGCGTATCTCAGTACGCTTAGTCTGGTGATGGGATGGGAAATGGGTAACGCGCCTGTTTATTTGTGGACTTTGCCGATGTTCCATTGTAATGGATGGACGTTCACGTGGGGGATTGCAGCTCGTGGTGGGACTAATGTCTGCATGCGTAATACGACGGCGTTTGATATTTACCGGAATATTAATTTGCATCATGTGACTCACATGTGCTGTGCGCCGATTGTGTTCCGTATCATCCTTGAGGCTAACGGCGGTGAACGGCGGCGATTTGGATGGCTAGTTAATGTGCTGACTGGCGGAGCGCCGCCACCGGCGGCGCTCCTGGAGAAAATGGAGGCTCTTGGGTTTCATATTACTCATGCTTATGGGCTGACGGAGGCCACCGGACCGGCGTTGGTCTGCGAGTGGCAAGAAAAATGGAATATTCTGCCGGCCGATGAACAAGCCAAGCTTAAAGCTAGACAAGGAATCAGCATTTTGACATTAGCTGACGTGGACGTCAAAAACTTGGAGACAATGGACAGCGTGCCACACGATGGTCGGACCACCGGCGAGATCGTCCTACGTGGCAGCAGTATCATGAAGGGCTACTTCAAGGATCCGGCAGCCACCGCAAAGGCCTTCCAAAATGGGTGGTTTCTCACCGGCGACGTCGGCGTGATACACCCAGATGGGTATTTGGAGATAAAGGACAGGTCGAAGGACGTCATCATCTCAGGCGGTGAGAACATCAGCAGCGTGGAGGTCGAGACGGTGCTGTACCGGCACCCACAGGTGGCGGAGGCAGCGGTGGTGGCAATGCCACACCCCCGGTGGGGTGAGAGCCCGTGCGCCTTCGTGGTGGTCCACAGCGACGGCGGCAAGGTGAGTGAGGCGGAGATGATTGAATTTTGCCGGAAGAATATGTCGCACTATATGGCGCCGAAGAAGGTGGTGTTCGTGGCGGAGCTTCCCAAGACGTTGACCGGAAAGATTCAGAAGTTCAAACTGAGGGAGCAGGCGAAATTGTTGGTTGGATCTGAGAAACGTGTCTCCGGCGGGAACAAGTCCGGGCGGCACGACGGTGGGCGGCAGCCGGATTACGGTCAGCATGTTATGGCCATGTCACGGCTTTGA

Protein sequence

MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLNVAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQFIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESPCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKLLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL
Homology
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BLAST of CmoCh04G023680 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 2.9e-214
Identity = 362/550 (65.82%), Postives = 429/550 (78.00%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M+ L KC ANY  LTP+TFL RA   YA+RTSIIY G RFTW QTY+RCCRLASSL  LN
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           + KN VVSVLAPNVPA YEMHFAVPMAGA+LNTINTRLD  NIA+IL+HSEAK+LFVDY+
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 FIQEAKDALRQLMAAS---------LPLPFVVVIDDIDSPTG-ARFGELEYEQLVHDGDV 180
           ++++A+ AL  LM+ +         + +P V++IDD+ SPT   +  +LEYEQLVH G+ 
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTRIQQQDQLEYEQLVHQGNP 180

Query: 181 GFTPVE-VDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTL 240
            + P   VDDEWD I LNYTSGTTS PKGVVYSHRGA+LSTL+ +MGWEMG  PVYLW+L
Sbjct: 181 EYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPVYLWSL 240

Query: 241 PMFHCNGWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGG 300
           PMFH NGWT TWGIAARGGTNVC+RNTTA +IY NI LH VTHMCCAP VF I+LEA   
Sbjct: 241 PMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILLEAKPH 300

Query: 301 ERRRFGWLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPA 360
           ERR     V V+ GGAPPP  L+ K+E LGFH+ H YG+TEA G  LVCEWQ +WN L  
Sbjct: 301 ERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEWNKLSR 360

Query: 361 DEQAKLKARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATA 420
           ++QA LKARQGIS+L L DVDVKN +TM SVPH+G+T GEI LRGSSIMKGYFK+  A +
Sbjct: 361 EDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKNDKANS 420

Query: 421 KAFQNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVV 480
           + F+NGWFLTGDV VIH DGYLEIKDR KD+IISGGENISS+EVE  + +HP V EAAVV
Sbjct: 421 QVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVIEAAVV 480

Query: 481 AMPHPRWGESPCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKI 540
           AMPHPRWGE+PCAF V+ +   ++ EA++I  C+K +  +M PK V F+ ELPKT TGK+
Sbjct: 481 AMPHPRWGETPCAF-VIKTKNPEIKEADIIVHCKKELPGFMVPKHVQFLEELPKTGTGKV 540

[Close]
BLAST of CmoCh04G023680 vs. ExPASy Swiss-Prot
Match: Q9SS01 (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 2.4e-200
Identity = 349/558 (62.54%), Postives = 426/558 (76.34%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M+ L+ C+AN   LTP+TFLKRAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           ++KN VVSV+APN PALYEMHFAVPMAGA+LN INTRLD  +IA ILRH++ KILF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 FIQEAKDALRQLMA--ASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPV--- 180
           F   A+++L  L +  ++L LP V+ I + D P  A F EL+YE L+  G+   + V   
Sbjct: 121 FEALARESLHLLSSEDSNLNLP-VIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARM 180

Query: 181 -EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHC 240
             + DE D I+LNYTSGTT+ PKGVV SHRGAYL TLS ++GWEMG  PVYLWTLPMFHC
Sbjct: 181 FRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHC 240

Query: 241 NGWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRF 300
           NGWTFTWG AARGGT+VCMR+ TA +IY+NI +H+VTHMCC P VF I+L+ N  +    
Sbjct: 241 NGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPR 300

Query: 301 GWLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAK 360
              V+VLTGG+PPPAAL++K++ LGF + HAYG TEATGP L CEWQ++WN LP ++Q +
Sbjct: 301 SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQME 360

Query: 361 LKARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQN 420
           LKARQGISIL LADVDVKN ET  S P DG+T GEI+++GSSIMKGY K+P AT +AF++
Sbjct: 361 LKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKH 420

Query: 421 GWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHP 480
           GW  TGDVGVIHPDG++EIKDRSKD+IISGGENISSVEVE VLY++P+V E AVVAMPHP
Sbjct: 421 GWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHP 480

Query: 481 RWGESPCAFVVVHS----------DGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKT 540
            WGE+PCAFVV+            D  +  E  +IE+CR+N+ H+M P+KVVF+ ELPK 
Sbjct: 481 TWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKN 540

Query: 541 LTGKIQKFKLREQAKLLV 543
             GKI K KLR+ AK LV
Sbjct: 541 GNGKILKPKLRDIAKGLV 557

[Close]
BLAST of CmoCh04G023680 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 7.0e-200
Identity = 342/552 (61.96%), Postives = 422/552 (76.45%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M++L  C+AN   LTPITFLKRAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           + +N VVS+LAPNVPA+YEMHF+VPM GA+LN INTRLD   IA+ILRH+E KILFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 FIQEAKDALRQLMA-ASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGD----VGFTPV 180
           F    ++ LR +    S P P +++I++IDS T     EL+YE L+  G+       +  
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            V +E D I+LNYTSGTT+ PKGVV SH+GAYLS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFG 300
           GWT TW +AARGGTNVC+R+ TA +IY+NI LH VTHM C P VFR +LE +  ++    
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 WLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKL 360
             V VLTGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ++WN LP  +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNG 420
           + RQG+  LTLADVDVKN +T++SVP DG+T GEIV++GSS+MKGY K+P AT++AF++G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+EVE VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVVVH--SDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFK 540
           WGE+PCAFVV+    +G   SE ++I++CR+NM H+M PKKVVF  ELPK   GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540

Query: 541 LREQAKLLVGSE 546
           LR+ AK LV  E
Sbjct: 541 LRDIAKALVVRE 552

[Close]
BLAST of CmoCh04G023680 vs. ExPASy Swiss-Prot
Match: Q9SS00 (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE12 PE=2 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 2.2e-198
Identity = 344/563 (61.10%), Postives = 429/563 (76.20%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M++L+ C+AN   LTPITFLKRAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           + KN VVSV+APN PA+YEMHFAVPMAGA+LN INTRLD  +IA ILRH++ KILF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 FIQEAKDALRQLMA--ASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVE-- 180
           F   A++ L+ L +  ++L LP V+ I +ID P      E +YE L+  G+   TP+   
Sbjct: 121 FEPLAREILQLLSSEDSNLNLP-VIFIHEIDFPKRVSSEESDYECLIQRGEP--TPLLLA 180

Query: 181 ----VDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMF 240
               + DE D I+LNYTSGTT+ PKGVV SHRGAYLSTLS ++GWEMG  PVYLWTLPMF
Sbjct: 181 RMFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMF 240

Query: 241 HCNGWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERR 300
           HCNGWTFTWG AARGGT+VCMR+ TA +IY+NI +H+VTHMCC P VF I+L+ N  +  
Sbjct: 241 HCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLS 300

Query: 301 RFGWLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQ 360
                V+VLTGG+PPPAAL++K++ LGF + HAYGLTEATGP L CEWQ++WN LP ++Q
Sbjct: 301 HRSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQ 360

Query: 361 AKLKARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAF 420
            +LKARQG+SIL L +VDV+N ET +SVP DG+T GEIV++GSSIMKGY K+P AT +AF
Sbjct: 361 MELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAF 420

Query: 421 QNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMP 480
           ++GW  +GDVGVIHPDG++EIKDRSKD+IISGGENISSVEVE ++Y++P+V E AVVAMP
Sbjct: 421 KHGWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMP 480

Query: 481 HPRWGESPCAFVVV---------HSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPK 540
           HP WGE+PCAFVV+           D     E ++IE+CR+N+ H+M P+KVVF+ ELPK
Sbjct: 481 HPTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPK 540

Query: 541 TLTGKIQKFKLREQAKLLVGSEK 547
              GKI K KLR+ AK LV  ++
Sbjct: 541 NGNGKILKPKLRDIAKGLVAEDE 560

[Close]
BLAST of CmoCh04G023680 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.7e-167
Identity = 291/551 (52.81%), Postives = 389/551 (70.60%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M    K  ANY  LTPI+FL R++  YADR SI+Y   ++TWRQT  RC R+AS+LS+L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           ++   VVSVLAPNVPA+ E+HF VPMAGA+L T+N R D + +A++LRHS  K++F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDD--IDSPTGARFGE--LEYEQLVHDGDVGFTPVE 180
           F+Q A+ A   L      +P +V+I +    S +  +  E  +EYE +V  G   F  + 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGAYL++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGW 300
           W   WG+ A GGTN+C+RN TA  I+ NI+ H VTHM  AP +  +I+ A   E++    
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 LVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GP  +C W+ +W+ LP +EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGW 420
           ARQG++ L L ++ VK+  TM ++P DG T GE+V RG+++M GY K+P AT +AF+ GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+EVE+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540
           GE+ CAFV +  DG K S  E+I +CR  + HYMAP+ +VF  +LPKT TGK+QKF LR 
Sbjct: 483 GETACAFVKL-KDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRT 542

Query: 541 QAKLLVGSEKR 548
           +AK LV   K+
Sbjct: 543 KAKALVSLSKK 551

[Close]
BLAST of CmoCh04G023680 vs. ExPASy TrEMBL
Match: A0A6J1ECD9 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431279 PE=4 SV=1)

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540
           CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 576
           LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL
Sbjct: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

[Close]
BLAST of CmoCh04G023680 vs. ExPASy TrEMBL
Match: A0A6J1JUR4 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111487678 PE=4 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 562/575 (97.74%), Postives = 569/575 (98.96%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           MNSLSKCDAN+ASLTPITFLKRASAFYADRTSIIYEGT FTWRQTY+RCC LASSLSRLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           V KNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDV+NIALILRHSEAKILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTNVC+RNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540
           CAFVVVHSD GKVSEAEMIEFCRKNMSHYMAPKKV+FVAELPKTLTGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 576
           LV SEKRVSGGNKSGRHDGGR P+YG+HVMAMSRL
Sbjct: 541 LVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSRL 575

[Close]
BLAST of CmoCh04G023680 vs. ExPASy TrEMBL
Match: A0A5A7TXW0 (Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold284G00160 PE=4 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 2.0e-290
Identity = 501/580 (86.38%), Postives = 530/580 (91.38%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M  L KCDANY +LTPITFLKRASAFYADRTSIIYEGTRFTW QTY+RCCRLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           V+KN VVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDV NIALILRHSEAKI FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           +IQEAKDALR L+A S  +P VVVIDDID+PTGAR G LEYEQL+HDGD  FTPVEVDDE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTN+CMRNTTA DI+RNINLH VTHMCCAPIVFRIILEA+ G+RRR  W VNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPAD+QA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSS+MKGYFKDP ATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDG--GKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQA 540
           CAFVV+H  G  G+VSEAEMI FCRKN+SHYM PKKVVF+AELPK   GK+QKF LREQA
Sbjct: 481 CAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPKA-NGKVQKFMLREQA 540

Query: 541 KLLVGSEK-RVSG-GNKSGRHDGGRQPDYG-QHVMAMSRL 576
           K L  SEK RVS  GNKS    G R+ DYG QHV+AMSRL
Sbjct: 541 KYLEVSEKRRVSNLGNKS----GNRRSDYGDQHVIAMSRL 575

[Close]
BLAST of CmoCh04G023680 vs. ExPASy TrEMBL
Match: A0A1S3CIM1 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103501376 PE=4 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 2.0e-290
Identity = 501/580 (86.38%), Postives = 530/580 (91.38%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M  L KCDANY +LTPITFLKRASAFYADRTSIIYEGTRFTW QTY+RCCRLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           V+KN VVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDV NIALILRHSEAKI FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           +IQEAKDALR L+A S  +P VVVIDDID+PTGAR G LEYEQL+HDGD  FTPVEVDDE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTN+CMRNTTA DI+RNINLH VTHMCCAPIVFRIILEA+ G+RRR  W VNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPAD+QA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSS+MKGYFKDP ATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDG--GKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQA 540
           CAFVV+H  G  G+VSEAEMI FCRKN+SHYM PKKVVF+AELPK   GK+QKF LREQA
Sbjct: 481 CAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPKA-NGKVQKFMLREQA 540

Query: 541 KLLVGSEK-RVSG-GNKSGRHDGGRQPDYG-QHVMAMSRL 576
           K L  SEK RVS  GNKS    G R+ DYG QHV+AMSRL
Sbjct: 541 KYLEVSEKRRVSNLGNKS----GNRRSDYGDQHVIAMSRL 575

[Close]
BLAST of CmoCh04G023680 vs. ExPASy TrEMBL
Match: A0A0A0KT40 (Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G319910 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.4e-288
Identity = 490/576 (85.07%), Postives = 527/576 (91.49%), Query Frame = 0 

Query: 4   LSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLNVAK 63
           L KCDANY++LTPITFLKRASAFYADRTSIIYEGTRFTW+QTY+RCCRLASSL RL V+K
Sbjct: 2   LLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLTVSK 61

Query: 64  NTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQFIQ 123
           N VVSVLAPNVPALYEMHFAVPMAGAILNTINTRLD+ NIALILRHSEAKI FVDYQ+IQ
Sbjct: 62  NDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQYIQ 121

Query: 124 EAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDEWDS 183
           EAKDALR L+A S P+P VVVIDDID+PTGAR G LEYEQL++DGD  FTPVEVDDEWDS
Sbjct: 122 EAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDEWDS 181

Query: 184 IALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 243
           IALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI
Sbjct: 182 IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 241

Query: 244 AARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNVLTG 303
           AARGGTN+CMRNTTA DI+RNINLH VTHMCCAPIVF IILEA+  +RRR  W VNVLTG
Sbjct: 242 AARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNVLTG 301

Query: 304 GAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQGISI 363
           GAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPAD+QA LKARQGISI
Sbjct: 302 GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQGISI 361

Query: 364 LTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTGDVG 423
           LTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSS+MKGYFKDPAATA A +NGWFLTGD+G
Sbjct: 362 LTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTGDIG 421

Query: 424 VIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESPCAF 483
           VI+PDGY+EIKDRSKDVIISGGENISSVEVETVLYRHPQV+EAAVVAMPHPRWGESPCAF
Sbjct: 422 VIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESPCAF 481

Query: 484 VVVHSDG--GKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKLL 543
           VV+   G  G+VSEAEM+ FCRKN+SHYM PKKVVFVAELPK   GK+QKF LREQAK L
Sbjct: 482 VVLQDGGGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPKA-NGKVQKFMLREQAKYL 541

Query: 544 VGSEKRV--SGGNKSGRHDGGRQPDYGQHVMAMSRL 576
             SEKR+  + GNKS    G R+ DYG+HV+AMSRL
Sbjct: 542 EVSEKRIVSNMGNKS----GNRRSDYGEHVIAMSRL 572

[Close]
BLAST of CmoCh04G023680 vs. NCBI nr
Match: XP_022923630.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540
           CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 576
           LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL
Sbjct: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

[Close]
BLAST of CmoCh04G023680 vs. NCBI nr
Match: KAG6602004.1 (Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 574/575 (99.83%), Postives = 574/575 (99.83%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKD AATAKAFQNGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDLAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540
           CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 576
           LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL
Sbjct: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

[Close]
BLAST of CmoCh04G023680 vs. NCBI nr
Match: XP_023513711.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 570/575 (99.13%), Postives = 574/575 (99.83%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTY+RCCRLASSLSRLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLSRLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           V KNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDV+NIALILRHSEAKILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLV+GWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVVGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540
           CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 576
           LVGSEKRVSGGNKSGRHDGGRQP+YGQHVMAMSRL
Sbjct: 541 LVGSEKRVSGGNKSGRHDGGRQPEYGQHVMAMSRL 575

[Close]
BLAST of CmoCh04G023680 vs. NCBI nr
Match: XP_022990938.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 562/575 (97.74%), Postives = 569/575 (98.96%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           MNSLSKCDAN+ASLTPITFLKRASAFYADRTSIIYEGT FTWRQTY+RCC LASSLSRLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           V KNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDV+NIALILRHSEAKILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTNVC+RNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540
           CAFVVVHSD GKVSEAEMIEFCRKNMSHYMAPKKV+FVAELPKTLTGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 LVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 576
           LV SEKRVSGGNKSGRHDGGR P+YG+HVMAMSRL
Sbjct: 541 LVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSRL 575

[Close]
BLAST of CmoCh04G023680 vs. NCBI nr
Match: XP_038906606.1 (trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 1021.9 bits (2641), Expect = 2.1e-294
Identity = 495/577 (85.79%), Postives = 526/577 (91.16%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M+ L KCDANY SLTPITFL RAS FYADRTSIIYEGTRFTW QTY+RCCRLASSL  LN
Sbjct: 1   MDMLLKCDANYTSLTPITFLTRASTFYADRTSIIYEGTRFTWHQTYERCCRLASSLRHLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           ++KN VVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIA+ILRHSEAKILFVDYQ
Sbjct: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIAVILRHSEAKILFVDYQ 120

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180
           +IQEAKDALR LMA S P+P VV+IDDIDSPTG RFG  EYE+L+HDGDV FTP+EVDDE
Sbjct: 121 YIQEAKDALRLLMAKSFPMPLVVIIDDIDSPTGIRFGNFEYEKLIHDGDVNFTPLEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300
           WGIAARGGTN+CMRNTTA DI+RNINLHHVTHMCCAPIVFRIIL+AN  +R R  W +NV
Sbjct: 241 WGIAARGGTNICMRNTTAIDIFRNINLHHVTHMCCAPIVFRIILDANSHDRCRVAWPINV 300

Query: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN LPAD+QAKLKARQG
Sbjct: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNTLPADKQAKLKARQG 360

Query: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDG TTGEIVLRGS +MKGYFKDP ATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLDTMESVPHDGHTTGEIVLRGSGLMKGYFKDPVATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVEVETVLY HPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYSHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540
           CAFV VH D G+VSEAE+I FCRKN+ HYM PKKVVFV ELPK   GKIQKFKLREQAK 
Sbjct: 481 CAFVAVHGDAGRVSEAEIIAFCRKNLPHYMVPKKVVFVGELPKA-NGKIQKFKLREQAKY 540

Query: 541 LVGSEKRV--SGGNKSGRHDGGRQPDYGQHVMAMSRL 576
           L  SEKRV  +G NKS R DG R+ DYG+HV+AMSRL
Sbjct: 541 LEVSEKRVVGTGWNKSSRQDGNRRSDYGEHVLAMSRL 576

[Close]
BLAST of CmoCh04G023680 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 699.9 bits (1805), Expect = 1.7e-201
Identity = 349/558 (62.54%), Postives = 426/558 (76.34%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M+ L+ C+AN   LTP+TFLKRAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           ++KN VVSV+APN PALYEMHFAVPMAGA+LN INTRLD  +IA ILRH++ KILF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 FIQEAKDALRQLMA--ASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPV--- 180
           F   A+++L  L +  ++L LP V+ I + D P  A F EL+YE L+  G+   + V   
Sbjct: 121 FEALARESLHLLSSEDSNLNLP-VIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARM 180

Query: 181 -EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHC 240
             + DE D I+LNYTSGTT+ PKGVV SHRGAYL TLS ++GWEMG  PVYLWTLPMFHC
Sbjct: 181 FRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHC 240

Query: 241 NGWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRF 300
           NGWTFTWG AARGGT+VCMR+ TA +IY+NI +H+VTHMCC P VF I+L+ N  +    
Sbjct: 241 NGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPR 300

Query: 301 GWLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAK 360
              V+VLTGG+PPPAAL++K++ LGF + HAYG TEATGP L CEWQ++WN LP ++Q +
Sbjct: 301 SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQME 360

Query: 361 LKARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQN 420
           LKARQGISIL LADVDVKN ET  S P DG+T GEI+++GSSIMKGY K+P AT +AF++
Sbjct: 361 LKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKH 420

Query: 421 GWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHP 480
           GW  TGDVGVIHPDG++EIKDRSKD+IISGGENISSVEVE VLY++P+V E AVVAMPHP
Sbjct: 421 GWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHP 480

Query: 481 RWGESPCAFVVVHS----------DGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKT 540
            WGE+PCAFVV+            D  +  E  +IE+CR+N+ H+M P+KVVF+ ELPK 
Sbjct: 481 TWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKN 540

Query: 541 LTGKIQKFKLREQAKLLV 543
             GKI K KLR+ AK LV
Sbjct: 541 GNGKILKPKLRDIAKGLV 557

[Close]
BLAST of CmoCh04G023680 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 698.4 bits (1801), Expect = 5.0e-201
Identity = 342/552 (61.96%), Postives = 422/552 (76.45%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M++L  C+AN   LTPITFLKRAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           + +N VVS+LAPNVPA+YEMHF+VPM GA+LN INTRLD   IA+ILRH+E KILFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 FIQEAKDALRQLMA-ASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGD----VGFTPV 180
           F    ++ LR +    S P P +++I++IDS T     EL+YE L+  G+       +  
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            V +E D I+LNYTSGTT+ PKGVV SH+GAYLS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFG 300
           GWT TW +AARGGTNVC+R+ TA +IY+NI LH VTHM C P VFR +LE +  ++    
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 WLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKL 360
             V VLTGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ++WN LP  +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNG 420
           + RQG+  LTLADVDVKN +T++SVP DG+T GEIV++GSS+MKGY K+P AT++AF++G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+EVE VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVVVH--SDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFK 540
           WGE+PCAFVV+    +G   SE ++I++CR+NM H+M PKKVVF  ELPK   GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540

Query: 541 LREQAKLLVGSE 546
           LR+ AK LV  E
Sbjct: 541 LRDIAKALVVRE 552

[Close]
BLAST of CmoCh04G023680 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 693.3 bits (1788), Expect = 1.6e-199
Identity = 344/563 (61.10%), Postives = 429/563 (76.20%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M++L+ C+AN   LTPITFLKRAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           + KN VVSV+APN PA+YEMHFAVPMAGA+LN INTRLD  +IA ILRH++ KILF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 FIQEAKDALRQLMA--ASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVE-- 180
           F   A++ L+ L +  ++L LP V+ I +ID P      E +YE L+  G+   TP+   
Sbjct: 121 FEPLAREILQLLSSEDSNLNLP-VIFIHEIDFPKRVSSEESDYECLIQRGEP--TPLLLA 180

Query: 181 ----VDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMF 240
               + DE D I+LNYTSGTT+ PKGVV SHRGAYLSTLS ++GWEMG  PVYLWTLPMF
Sbjct: 181 RMFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMF 240

Query: 241 HCNGWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERR 300
           HCNGWTFTWG AARGGT+VCMR+ TA +IY+NI +H+VTHMCC P VF I+L+ N  +  
Sbjct: 241 HCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLS 300

Query: 301 RFGWLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQ 360
                V+VLTGG+PPPAAL++K++ LGF + HAYGLTEATGP L CEWQ++WN LP ++Q
Sbjct: 301 HRSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQ 360

Query: 361 AKLKARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAF 420
            +LKARQG+SIL L +VDV+N ET +SVP DG+T GEIV++GSSIMKGY K+P AT +AF
Sbjct: 361 MELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAF 420

Query: 421 QNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMP 480
           ++GW  +GDVGVIHPDG++EIKDRSKD+IISGGENISSVEVE ++Y++P+V E AVVAMP
Sbjct: 421 KHGWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMP 480

Query: 481 HPRWGESPCAFVVV---------HSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPK 540
           HP WGE+PCAFVV+           D     E ++IE+CR+N+ H+M P+KVVF+ ELPK
Sbjct: 481 HPTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPK 540

Query: 541 TLTGKIQKFKLREQAKLLVGSEK 547
              GKI K KLR+ AK LV  ++
Sbjct: 541 NGNGKILKPKLRDIAKGLVAEDE 560

[Close]
BLAST of CmoCh04G023680 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 590.1 bits (1520), Expect = 1.9e-168
Identity = 291/551 (52.81%), Postives = 389/551 (70.60%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
           M    K  ANY  LTPI+FL R++  YADR SI+Y   ++TWRQT  RC R+AS+LS+L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
           ++   VVSVLAPNVPA+ E+HF VPMAGA+L T+N R D + +A++LRHS  K++F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 FIQEAKDALRQLMAASLPLPFVVVIDD--IDSPTGARFGE--LEYEQLVHDGDVGFTPVE 180
           F+Q A+ A   L      +P +V+I +    S +  +  E  +EYE +V  G   F  + 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGAYL++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGW 300
           W   WG+ A GGTN+C+RN TA  I+ NI+ H VTHM  AP +  +I+ A   E++    
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 LVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GP  +C W+ +W+ LP +EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGW 420
           ARQG++ L L ++ VK+  TM ++P DG T GE+V RG+++M GY K+P AT +AF+ GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+EVE+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540
           GE+ CAFV +  DG K S  E+I +CR  + HYMAP+ +VF  +LPKT TGK+QKF LR 
Sbjct: 483 GETACAFVKL-KDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRT 542

Query: 541 QAKLLVGSEKR 548
           +AK LV   K+
Sbjct: 543 KAKALVSLSKK 551

[Close]
BLAST of CmoCh04G023680 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 574.3 bits (1479), Expect = 1.1e-163
Identity = 292/545 (53.58%), Postives = 373/545 (68.44%), Query Frame = 0 

Query: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIY-EGTRFTWRQTYQRCCRLASSLSRL 60
           M  +  C AN   LTPI FL+RA+  Y D TSI+Y   T +TWR+T  RC R+ASSLS +
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  NVAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDY 120
            + ++ VVSVL+PN PA+YE+ FAVPM+GAILN INTRLD   ++++LRH  +K+LFVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 QFIQEAKDALRQLMAASLPLPFVVVID----DIDSPTGARFGELEYEQLVHDGDVGFTPV 180
             +  A +A+  +      L F+   +    D D     +F    Y+ L+H GD+ F  +
Sbjct: 121 FSVDLAVEAISMMTTDPPILVFIADKEEEGGDADVADRTKF-SYTYDDLIHRGDLDFKWI 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
             + EWD + LNYTSGTTSAPKGVV+ HRG ++ ++  ++ W +   PVYLWTLP+FH N
Sbjct: 181 RPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFHAN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFG 300
           GW++ WGIAA GGTNVC+R   A  IYR I  H VTHMC AP+V  ++   N  E +   
Sbjct: 241 GWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATN--EFQPLN 300

Query: 301 WLVNVLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKL 360
             VN+LT GAPPPAA+L + E++GF I+H YGLTE  G  + C W+ +WN LPA ++A+L
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KARQGISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNG 420
           KARQG+  +   ++DV + E+  SV  +G T GEIV+RGSSIM GY KDP  T KA +NG
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKNG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPR 480
           WF TGDVGVIH DGYLEIKDRSKD+II+GGEN+SSVEVETVLY +P V E AVVA P   
Sbjct: 421 WFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDVF 480

Query: 481 WGESPCAFVVVHSD-GGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKL 540
           WGE+PCAFV + S    + +E EMIE+CRK M  YM PK V FV ELPKT TGK+ KF L
Sbjct: 481 WGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFVL 540

The following BLAST results are available for this feature:
BLAST of CmoCh04G023680 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
I3PB362.9e-21465.82Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 S... [more]
Q9SS012.4e-20062.54Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 ... [more]
Q9C8D47.0e-20061.96Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11... [more]
Q9SS002.2e-19861.10Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 ... [more]
F4HUK62.7e-16752.81Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1[more]
BLAST of CmoCh04G023680 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1ECD90.0e+00100.00butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JUR40.0e+0097.74butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5A7TXW02.0e-29086.38Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CIM12.0e-29086.38butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A0A0KT405.4e-28885.07Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 G... [more]
BLAST of CmoCh04G023680 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022923630.10.0e+00100.00butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata][more]
KAG6602004.10.0e+0099.83Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023513711.10.0e+0099.13butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo][more]
XP_022990938.10.0e+0097.74butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima][more]
XP_038906606.12.1e-29485.79trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida][more]
BLAST of CmoCh04G023680 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G65880.11.7e-20162.54benzoyloxyglucosinolate 1 [more]
AT1G66120.15.0e-20161.96AMP-dependent synthetase and ligase family protein [more]
AT1G65890.11.6e-19961.10acyl activating enzyme 12 [more]
AT1G20560.11.9e-16852.81acyl activating enzyme 1 [more]
AT5G16370.11.1e-16353.58acyl activating enzyme 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.30coord: 439..540
e-value: 1.2E-32
score: 114.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 545..575
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 1..547
NoneNo IPR availablePANTHERPTHR43859:SF2BUTYRATE--COA LIGASE AAE11, PEROXISOMALcoord: 1..547
NoneNo IPR availableCDDcd12118ttLC_FACS_AEE21_likecoord: 11..536
e-value: 0.0
score: 840.42
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 14..539
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 3..438
e-value: 2.9E-99
score: 334.4
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 452..528
e-value: 2.3E-24
score: 86.1
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 22..443
e-value: 1.5E-94
score: 317.0
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 186..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G023680.1CmoCh04G023680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity