CmoCh04G023620 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh04G023620 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 7.1e-234
Identity = 385/548 (70.26%), Postives = 458/548 (83.58%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           MEDLKPRPA+SSPLTP+GFLERAAT+YGD  S++Y   +YTWS THRRCL LASS++S+G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           I+ GHVVSV++PN P MYELHFAVPM GAILN +N RLDARTISILL HSESKLIFVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
             +LI  A+ LFP +   P LV +AD++   ES +S  +   F  SY+++I +GD DF+W
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADES---ESGNSSELGKEFFCSYKDLIDRGDPDFKW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           + P SEWDP+ LNYTSGTTSSPKGVVHCHRG FI+T+D L++WGVPKQ VYLWTLPMFHA
Sbjct: 181 VMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWS+ WGMAAVGGTNIC+RKFD+ ++Y +I+RH VTH+CGAPVVLNML+N+P   PL  
Sbjct: 241 NGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKT 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
            V+I+TAGAP P+ +LFR ESLGF V+HGYGLTET G VV C+WK EW+ LPA ERARLK
Sbjct: 301 TVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGV TV    +DVVD  TG +V RDG ++GEVVL+ GS+MLGY KD E TAKSMT +G
Sbjct: 361 SRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WF+TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILY++P + EAAVVARP EF
Sbjct: 421 WFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSL++GLT++PTE+EI+EYC+ KLPR+M PKTVVF  ELPKTSTGK+QK++L
Sbjct: 481 WGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFIL 540

Query: 541 KEMAMSMG 549
           ++MA  MG
Sbjct: 541 RDMARGMG 545

BLAST of CmoCh04G023620 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 5.3e-213
Identity = 352/550 (64.00%), Postives = 440/550 (80.00%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSS-I 60
           ME+LKPR ANS PLTP+GFLERAAT+YGD  S++Y   +YTWS TH RCL++AS + S +
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           G+++G VVSV++PN P MYEL+FAVPM G +LN INTRL+A TIS++L HSESKL+FVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 AFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKG-DSDF 180
               L+ +A+ LFP     P+LVLIAD+   E  + +V    +FV  YE+++ KG D  F
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGE--NLTVEDREHFVCCYEDLVEKGDDKTF 180

Query: 181 RWIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMF 240
           +W+RP SEWDPI LNYTSGTTSSPKGVVH HR  F++T+D L++WGVPKQ VYLWTLPMF
Sbjct: 181 KWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPL 300
           HANGW ++WG+AAVGGTNIC+R+FD  ++++LIRRH VTH+C AP+VLNML+NSP+  PL
Sbjct: 241 HANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PV ++T GAP PA +L R ESLGF ++HGYG+TE  G VV C+WK EW++LPA E+AR
Sbjct: 301 PNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQAR 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTE 420
           LKSRQGVRT A   VDVVD  +G SV RDGL+MGE+VLK  S+MLGY K+  ATAK +  
Sbjct: 361 LKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRA 420

Query: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPH 480
           +GWF+TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++P V+EAAVVA P 
Sbjct: 421 DGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPD 480

Query: 481 EFWGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           E+WGETP AFV+L++G+  +PTE+EI+EYC+ KL  FM PK VVF  +LPKTSTGKIQK+
Sbjct: 481 EYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKF 540

Query: 541 LLKEMAMSMG 549
            LKE+  +MG
Sbjct: 541 TLKEIVKTMG 548

BLAST of CmoCh04G023620 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.6e-209
Identity = 350/549 (63.75%), Postives = 438/549 (79.78%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIY-AHTTYTWSHTHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGFLERAAT+YGD  SI+Y ++T YTW  T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 AFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFR 180
              +L   A+ +     + P+LV IAD    E   + V     F  +Y+++I +GD DF+
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIADKE-EEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+ LNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           ANGWS+ WG+AAVGGTN+C+RKFDA L+Y LIR H VTH+CGAPVVLNML+ + + +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAGAP PA +L R ES+GF ++HGYGLTET G  V C+WK +W++LPA +RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEE 420
           K+RQGVRTV    +DVVD E+G SV R+G ++GE+V++  S+MLGY KD   T K++ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYTNP VNE AVVARP  
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 481 FWGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ GLT++PTE E+IEYC+ K+P++M PKTV F+ ELPKTSTGK+ K++
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 541 LKEMAMSMG 549
           L+E+A  MG
Sbjct: 541 LREIAKKMG 545

BLAST of CmoCh04G023620 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 3.3e-207
Identity = 345/549 (62.84%), Postives = 439/549 (79.96%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIY-AHTTYTWSHTHRRCLQLASSLSSI 60
           ME++KP  ANS PLTPIGFLERAAT+YGD  SI+Y ++T YTW  T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 AFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFR 180
              +L   A+ +     + P+LV+IAD    E   + V     F  +Y+++I +GD  F+
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIADKE-EEGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+ LNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           +NGW+  WG+AAVGGTN+C+RKFDA L+Y LIR H VTH+CGAPVVLNML+ + + +PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAG+P PAT+L R ES+GF ++HGYGLTET G +V C+WK +W+ LPA +RARL
Sbjct: 301 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEE 420
           K+RQGVRTV    +DVVD E+G SV R+G ++GE+V++  S+MLGY KD   T K++ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYT P VNE AVVARP E
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ G + +PTEEE++EYC+ K+P++M PKTV FM ELPK+STGK+ K++
Sbjct: 481 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 540

Query: 541 LKEMAMSMG 549
           L+++A  MG
Sbjct: 541 LRDIAKKMG 545

BLAST of CmoCh04G023620 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.0e-204
Identity = 353/549 (64.30%), Postives = 432/549 (78.69%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTT-YTWSHTHRRCLQLASSLSSI 60
           MEDLKP  ANS PLT +GFLERAAT+YGD  SI+Y ++T YTW  T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 AFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFR 180
            + +L   A+ +    +N P+LVLIA++   EE  + V     F   Y ++I +G+ DF+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEE-EEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDPI +NYTSGTTSSPKGVVHCHRG F++T+D L +W VPK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDI-RPL 300
           ANGW++ WG+AAVGGTN+C+RK  A  +Y LIR H VTH+ GAP+VL +L+ S +  +PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PV  LTAG+  PAT+L R ESLGF V+HGYGLTET G +V C+WK  W++LPA ++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTE 420
           LKSRQGVRTV    +DVVD E+G SV RDG ++GE+VL+  S+MLGY K+   T  S  +
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPH 480
            GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE++LYTNP VNEAAVVARP 
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAFVSL+ GLT +PT++EIIEYCK K+PR+MAPKTV F+ ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 541 LLKEMAMSM 548
           LLKE+A +M
Sbjct: 541 LLKEIAKNM 544

BLAST of CmoCh04G023620 vs. ExPASy TrEMBL
Match: A0A6J1E3B0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430201 PE=4 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW
Sbjct: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGPP 551
           KEMAMSMGPP
Sbjct: 541 KEMAMSMGPP 550

BLAST of CmoCh04G023620 vs. ExPASy TrEMBL
Match: A0A6J1JUG4 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487609 PE=4 SV=1)

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 522/550 (94.91%), Postives = 539/550 (98.00%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           ME LKPRPANSSPLTPIGFLERAATIY DSPSIIY +TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVD+A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FCNLIHNALDLFPPE+NRPMLVL+ADDA AEESSSSVLIEGNFV SYE MIRKGDS+F+W
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPI LNYTSGTTSSPKGVVHCHRGSFI+T+DMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWGMAAVGGTNICIRKFDA LVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATILFRIESLGF+VTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAVDVVDLETGESVARDG+SMGE+VL+VGSLMLGYFKDLEATAKSMTE+G
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP+VNEAAVVARPHEF
Sbjct: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT+QPTEEEIIEYC+GKLPRFMAPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGPP 551
           KEMAMSMGPP
Sbjct: 541 KEMAMSMGPP 550

BLAST of CmoCh04G023620 vs. ExPASy TrEMBL
Match: A0A6J1JT47 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487610 PE=4 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 5.3e-309
Identity = 519/549 (94.54%), Postives = 535/549 (97.45%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           MEDLKPRPANSSPLTPIGFLERAATIY DSPSIIY +TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCH ESKLIFVD A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHGESKLIFVDHA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FCNLIHNALDLFPPE+ RPMLVLIADDA AEESSSSVLIEGNFV SYE MIRKGDS+F+W
Sbjct: 121 FCNLIHNALDLFPPEIYRPMLVLIADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPI LNYTSGTTSSPKGVVHCHRGSFI+T+DMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWGMAAVGGTNICIRKFDA LVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATILFRIESLGF+VTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAVDVVDLETGESVARDG+SMGE+VL+VGSLMLGYFKDLEATAKSMTE+G
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP+VNEAAVVARPHEF
Sbjct: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT+Q TEEEIIEYC+GKLPRFMAPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQRTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGP 550
           +EMAMSMGP
Sbjct: 541 REMAMSMGP 549

BLAST of CmoCh04G023620 vs. ExPASy TrEMBL
Match: A0A6J1E5Q3 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430192 PE=4 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 5.9e-308
Identity = 515/549 (93.81%), Postives = 539/549 (98.18%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           ME+LKPRPANSSPLTP+GFLERAATIYGDS SIIY HTTYTWSHTHRRCLQLASSLSSIG
Sbjct: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FCNLIHN+LDLFPPE+NRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDS+F+W
Sbjct: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPI LNYTSGTTSSPKGVVHCHRGSFI+T+DMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWG+AAVGGTNICIRKFD+ LVYSLIRRHHVTHLCGAPVVLNMLTNSP+IRPLDW
Sbjct: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATIL RIESLGF+VTHGYGLTETGGTVVCCSWKAEW+QLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAV+VVDLETG+SVARDG+S+GE+VL+VGSLMLGYFKDLEAT KSMTEEG
Sbjct: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNP+VNEAAVVARP EF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT++PTEEEIIE+CK KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGP 550
           +EMAMSM P
Sbjct: 541 REMAMSMDP 549

BLAST of CmoCh04G023620 vs. ExPASy TrEMBL
Match: A0A6J1JKT9 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487797 PE=4 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 7.7e-276
Identity = 467/549 (85.06%), Postives = 499/549 (90.89%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           MEDLKPRPANSSPLTP+GFLERAATIYGDSPSIIY  TTYTWS TH RCLQLASSLSS+G
Sbjct: 70  MEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHCRCLQLASSLSSMG 129

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGH+VSVISPNTPPMYELHFAVPM GAILNTINTRLDARTISILL HSESKLIFVDQA
Sbjct: 130 IQRGHIVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLTHSESKLIFVDQA 189

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSS-VLIEGNFVGSYEEMIRKGDSDFR 180
           FC LIH+AL LFPPE  RPMLVLI DDA AEESSSS ++I  +FV SYEEMI KGDSDFR
Sbjct: 190 FCALIHDALALFPPETKRPMLVLITDDAAAEESSSSCIMIHDDFVDSYEEMILKGDSDFR 249

Query: 181 WIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDPI LNYTSGTTSSPKGVV CHRG FIIT+D+LLEW V KQSVYLWTLPMFH
Sbjct: 250 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVQKQSVYLWTLPMFH 309

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
            NGWS +WGMAAVGGTNICIRKFDA L+Y+LIRRHHVTHLCGAPVVLNMLTNSP  RP D
Sbjct: 310 GNGWSLTWGMAAVGGTNICIRKFDAALIYTLIRRHHVTHLCGAPVVLNMLTNSPTNRPFD 369

Query: 301 WPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
           WPVKILT GAP P+ +L R ESLGF+VTHGYGLTET G VV CSWKAEW+QLP  ERARL
Sbjct: 370 WPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTKERARL 429

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEE 420
           KSRQGVRTVA  AVDVVD +TG+SV +DG+S+GE+VLK GS+MLGY KD +AT KSMTEE
Sbjct: 430 KSRQGVRTVAMTAVDVVDQKTGKSVKQDGVSIGEIVLKGGSIMLGYLKDRDATTKSMTEE 489

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHE 480
           GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP+VNEAAVVARP E
Sbjct: 490 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDE 549

Query: 481 FWGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVS+REGLT++PTEEEIIE+C+GKLPRFM PKTVVFMTELPKTSTGKIQKYL
Sbjct: 550 FWGETPCAFVSIREGLTQKPTEEEIIEHCRGKLPRFMVPKTVVFMTELPKTSTGKIQKYL 609

Query: 541 LKEMAMSMG 549
           L+EMAMSMG
Sbjct: 610 LREMAMSMG 618

BLAST of CmoCh04G023620 vs. NCBI nr
Match: XP_022922136.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW
Sbjct: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGPP 551
           KEMAMSMGPP
Sbjct: 541 KEMAMSMGPP 550

BLAST of CmoCh04G023620 vs. NCBI nr
Match: KAG6601999.1 (putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 534/550 (97.09%), Postives = 545/550 (99.09%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           MEDLKPRPANSSPLTPIGFLERAA IYGDSPSIIY HTTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAAIIYGDSPSIIYGHTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FCNLIHNALDLFPPE+NRPMLVLIADDAVAEESSSSVLI+G+F+GSYEEMIRKGDSDFRW
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLIADDAVAEESSSSVLIDGDFLGSYEEMIRKGDSDFRW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWGMAAVGGTNICIRKFDA LVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAALVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATILFRIESLGF+VTHGYGLTETGGTVVCCSWKAEW+QLPA ERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAEERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAVDVVDLETGESVARDG+SMGE+VL+VGSLMLGYFKDLEATAKSMTEEG
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNP+VNEAAVVARPHEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT+QPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGPP 551
           KEMAMSMGPP
Sbjct: 541 KEMAMSMGPP 550

BLAST of CmoCh04G023620 vs. NCBI nr
Match: XP_022990838.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 522/550 (94.91%), Postives = 539/550 (98.00%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           ME LKPRPANSSPLTPIGFLERAATIY DSPSIIY +TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVD+A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FCNLIHNALDLFPPE+NRPMLVL+ADDA AEESSSSVLIEGNFV SYE MIRKGDS+F+W
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPI LNYTSGTTSSPKGVVHCHRGSFI+T+DMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWGMAAVGGTNICIRKFDA LVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATILFRIESLGF+VTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAVDVVDLETGESVARDG+SMGE+VL+VGSLMLGYFKDLEATAKSMTE+G
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP+VNEAAVVARPHEF
Sbjct: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT+QPTEEEIIEYC+GKLPRFMAPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGPP 551
           KEMAMSMGPP
Sbjct: 541 KEMAMSMGPP 550

BLAST of CmoCh04G023620 vs. NCBI nr
Match: XP_023525395.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1074.7 bits (2778), Expect = 4.2e-310
Identity = 524/550 (95.27%), Postives = 535/550 (97.27%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIY  TTYTWS TH RCLQLASSLSS G
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYGDTTYTWSETHHRCLQLASSLSSSG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPM GAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FC++IHNALDLFPPE+NRPMLVLIADDAVAEESSSSVL +GNFVG YEEMIRKGDSDFRW
Sbjct: 121 FCDIIHNALDLFPPEINRPMLVLIADDAVAEESSSSVLSQGNFVGLYEEMIRKGDSDFRW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           I PASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IFPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWGMAAVGGTNICIRKFDA LVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAALVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATILFRIESLGF+VTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR+K
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARIK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAVDVVDLETGESVARDG+SMGE+VLKVGSLMLGYFKDLEATAKSMTEEG
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLKVGSLMLGYFKDLEATAKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WF TGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESILY NP+VNE AVVARPHEF
Sbjct: 421 WFLTGDVGVMHRDGYLEIKDRSKDVIISGGENLSSVEVESILYANPSVNEVAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT++PTEEEIIEYCK KLPRFMAPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQKPTEEEIIEYCKRKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGPP 551
           KEMAMSMGPP
Sbjct: 541 KEMAMSMGPP 550

BLAST of CmoCh04G023620 vs. NCBI nr
Match: XP_022990839.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.1e-308
Identity = 519/549 (94.54%), Postives = 535/549 (97.45%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60
           MEDLKPRPANSSPLTPIGFLERAATIY DSPSIIY +TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCH ESKLIFVD A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHGESKLIFVDHA 120

Query: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180
           FCNLIHNALDLFPPE+ RPMLVLIADDA AEESSSSVLIEGNFV SYE MIRKGDS+F+W
Sbjct: 121 FCNLIHNALDLFPPEIYRPMLVLIADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180

Query: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPI LNYTSGTTSSPKGVVHCHRGSFI+T+DMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSFSWGMAAVGGTNICIRKFDA LVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAPVPATILFRIESLGF+VTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420
           SRQGVRTVATPAVDVVDLETGESVARDG+SMGE+VL+VGSLMLGYFKDLEATAKSMTE+G
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP+VNEAAVVARPHEF
Sbjct: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT+Q TEEEIIEYC+GKLPRFMAPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQRTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 KEMAMSMGP 550
           +EMAMSMGP
Sbjct: 541 REMAMSMGP 549

BLAST of CmoCh04G023620 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 730.3 bits (1884), Expect = 1.1e-210
Identity = 350/549 (63.75%), Postives = 438/549 (79.78%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIY-AHTTYTWSHTHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGFLERAAT+YGD  SI+Y ++T YTW  T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 AFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFR 180
              +L   A+ +     + P+LV IAD    E   + V     F  +Y+++I +GD DF+
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIADKE-EEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+ LNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           ANGWS+ WG+AAVGGTN+C+RKFDA L+Y LIR H VTH+CGAPVVLNML+ + + +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAGAP PA +L R ES+GF ++HGYGLTET G  V C+WK +W++LPA +RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEE 420
           K+RQGVRTV    +DVVD E+G SV R+G ++GE+V++  S+MLGY KD   T K++ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYTNP VNE AVVARP  
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 481 FWGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ GLT++PTE E+IEYC+ K+P++M PKTV F+ ELPKTSTGK+ K++
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 541 LKEMAMSMG 549
           L+E+A  MG
Sbjct: 541 LREIAKKMG 545

BLAST of CmoCh04G023620 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 722.6 bits (1864), Expect = 2.3e-208
Identity = 345/549 (62.84%), Postives = 439/549 (79.96%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIY-AHTTYTWSHTHRRCLQLASSLSSI 60
           ME++KP  ANS PLTPIGFLERAAT+YGD  SI+Y ++T YTW  T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 AFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFR 180
              +L   A+ +     + P+LV+IAD    E   + V     F  +Y+++I +GD  F+
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIADKE-EEGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+ LNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           +NGW+  WG+AAVGGTN+C+RKFDA L+Y LIR H VTH+CGAPVVLNML+ + + +PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAG+P PAT+L R ES+GF ++HGYGLTET G +V C+WK +W+ LPA +RARL
Sbjct: 301 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEE 420
           K+RQGVRTV    +DVVD E+G SV R+G ++GE+V++  S+MLGY KD   T K++ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYT P VNE AVVARP E
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ G + +PTEEE++EYC+ K+P++M PKTV FM ELPK+STGK+ K++
Sbjct: 481 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 540

Query: 541 LKEMAMSMG 549
           L+++A  MG
Sbjct: 541 LRDIAKKMG 545

BLAST of CmoCh04G023620 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 713.4 bits (1840), Expect = 1.4e-205
Identity = 353/549 (64.30%), Postives = 432/549 (78.69%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTT-YTWSHTHRRCLQLASSLSSI 60
           MEDLKP  ANS PLT +GFLERAAT+YGD  SI+Y ++T YTW  T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 AFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFR 180
            + +L   A+ +    +N P+LVLIA++   EE  + V     F   Y ++I +G+ DF+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEE-EEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDPI +NYTSGTTSSPKGVVHCHRG F++T+D L +W VPK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDI-RPL 300
           ANGW++ WG+AAVGGTN+C+RK  A  +Y LIR H VTH+ GAP+VL +L+ S +  +PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PV  LTAG+  PAT+L R ESLGF V+HGYGLTET G +V C+WK  W++LPA ++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTE 420
           LKSRQGVRTV    +DVVD E+G SV RDG ++GE+VL+  S+MLGY K+   T  S  +
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPH 480
            GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE++LYTNP VNEAAVVARP 
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAFVSL+ GLT +PT++EIIEYCK K+PR+MAPKTV F+ ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 541 LLKEMAMSM 548
           LLKE+A +M
Sbjct: 541 LLKEIAKNM 544

BLAST of CmoCh04G023620 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 693.0 bits (1787), Expect = 2.0e-199
Identity = 338/555 (60.90%), Postives = 425/555 (76.58%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIY-AHTTYTWSHTHRRCLQLASSL--S 60
           ME L P P+NS+PLT +GFL+RAA++YGD PSI++  +T +TWS TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFV 120
           S+GI RG VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S+LL HSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQAFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSD 180
           D    +++  A+  F  +  +P LVL+ DD   E+ S S     +F+ +Y+ ++ +GDS 
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAASDFLDTYQGVMERGDSR 180

Query: 181 FRWIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPM 240
           F+WIRP +EW P+ LNYTSGTTSSPKGVV  HR  F++T+  LL+W  P + VYLWTLPM
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240

Query: 241 FHANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRP 300
           FHANGW ++WG AAVG TN+C R+ DA  +Y LI +HHVTH+C AP+VLNMLTN P  +P
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300

Query: 301 LDWPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERA 360
           L  PV+++TAGAP PA I+ R E+LGF V HGYGLTETGG VV C+WKAEWD L  +ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360

Query: 361 RLKSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMT 420
           RLKSRQGVRT+    VDV D  TG+SV  DG+S+GE+VLK GS+MLGY+KD E TA  M 
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420

Query: 421 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARP 480
           E+GWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE++LYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480

Query: 481 HEFWGETPCAFVSLR-----EGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTST 540
            + WGETPCAFVSL+      GL    TE EI E+CK +LP++M P+ V+F  ELPKTST
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKIQKYLLKEMAMSM 548
           GKIQK+LL++MA S+
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of CmoCh04G023620 vs. TAIR 10
Match: AT1G21530.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 683.7 bits (1763), Expect = 1.2e-196
Identity = 333/554 (60.11%), Postives = 428/554 (77.26%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIY-AHTTYTWSHTHRRCLQLASSL--S 60
           ME L P P+NS+PLT +GFL+RAA++YGD PSI++ A+T +TWS TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFV 120
           SIGI++G VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S+LL HSES+L+FV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQAFCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSD 180
           D    +L+  A+ LF  +  +P LVL+ DD   + SS+S     +F+ +YEE++ +G+S 
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLLDDDQENDSSSAS-----DFLDTYEEIMERGNSR 180

Query: 181 FRWIRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPM 240
           F+WIRP +EW P+ LNYTSGTTSSPKGVV  HR  F++T+  LL+W VP + VYLWTLPM
Sbjct: 181 FKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTLPM 240

Query: 241 FHANGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRP 300
           FHANGW ++WG AAVG TNIC R+ DA  +Y+LI +H+VTH+C AP+VLNML N P   P
Sbjct: 241 FHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLSTP 300

Query: 301 LDWPVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERA 360
           L  PV+++T+GAP PATI+ R ESLGF V+H YGLTET G VV C+WK +WD L  +ERA
Sbjct: 301 LKNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLERA 360

Query: 361 RLKSRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMT 420
           RLKSRQGVRT+    VDV D +TG+SV  DG+S+GE+V +  S+MLGY+KD + TA  M 
Sbjct: 361 RLKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAACMR 420

Query: 421 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARP 480
           E+GWF++GD+GV+H DGYLEIKDRSKDVII GGEN+SS E+E++LYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKP 480

Query: 481 HEFWGETPCAFVSLR---EGLTEQP-TEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTG 540
            + WGETPCAFVSL+    G    P TE EI E+CK KLP++M P+ V+F  ELPKTSTG
Sbjct: 481 DKMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTG 540

Query: 541 KIQKYLLKEMAMSM 548
           KIQK+LL++MA ++
Sbjct: 541 KIQKFLLRQMAKTL 548