CmoCh04G021950 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh04G021950
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Calcium-binding EF-hand family protein
Location	Cmo_Chr04: 16276001 .. 16276423 (-)
RNA-Seq Expression	CmoCh04G021950
Synteny	CmoCh04G021950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCCAATTCTCGCGCTACCAGAGAGTGTTCAACAGTATAGACTCCGACGGCGACGGCAAGGTCTCCTCTCAAGATTTGCTTACCCGGTTCGCGGCGGTGACAGGGGAGGAGCTCACGGAGGAGGAGGCGGCAGCGGTGGTGGCGGCCTACGATTCCGACGACGACGGGCTATTGGAGATTGCGGAGTTTGAGAGGTTGGTGGAGGATGGGGATGGAGAGGGGAAGGAGATAAAGGAGGCTTTCAAGATGTATGAAATGGAGGGATTGGGTGTGATTACGGCGGTGAGCCTGCGGCGGATGCTGAGCCAGCTGGGGCAATCGAGGAGCATCGGGGATTGCAACAGAATGATCGCTAAGTTTGATGTCAATGGCGATGGAGTTCTCTGTTTTGATGAGTTCAGGATTATGATGTTGCTCTGA

mRNA sequence

ATGCCCCAATTCTCGCGCTACCAGAGAGTGTTCAACAGTATAGACTCCGACGGCGACGGCAAGGTCTCCTCTCAAGATTTGCTTACCCGGTTCGCGGCGGTGACAGGGGAGGAGCTCACGGAGGAGGAGGCGGCAGCGGTGGTGGCGGCCTACGATTCCGACGACGACGGGCTATTGGAGATTGCGGAGTTTGAGAGGTTGGTGGAGGATGGGGATGGAGAGGGGAAGGAGATAAAGGAGGCTTTCAAGATGTATGAAATGGAGGGATTGGGTGTGATTACGGCGGTGAGCCTGCGGCGGATGCTGAGCCAGCTGGGGCAATCGAGGAGCATCGGGGATTGCAACAGAATGATCGCTAAGTTTGATGTCAATGGCGATGGAGTTCTCTGTTTTGATGAGTTCAGGATTATGATGTTGCTCTGA

Coding sequence (CDS)

ATGCCCCAATTCTCGCGCTACCAGAGAGTGTTCAACAGTATAGACTCCGACGGCGACGGCAAGGTCTCCTCTCAAGATTTGCTTACCCGGTTCGCGGCGGTGACAGGGGAGGAGCTCACGGAGGAGGAGGCGGCAGCGGTGGTGGCGGCCTACGATTCCGACGACGACGGGCTATTGGAGATTGCGGAGTTTGAGAGGTTGGTGGAGGATGGGGATGGAGAGGGGAAGGAGATAAAGGAGGCTTTCAAGATGTATGAAATGGAGGGATTGGGTGTGATTACGGCGGTGAGCCTGCGGCGGATGCTGAGCCAGCTGGGGCAATCGAGGAGCATCGGGGATTGCAACAGAATGATCGCTAAGTTTGATGTCAATGGCGATGGAGTTCTCTGTTTTGATGAGTTCAGGATTATGATGTTGCTCTGA

Protein sequence

MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNGDGVLCFDEFRIMMLL

Homology

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BLAST of CmoCh04G021950 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 5.4e-29
Identity = 70/135 (51.85%), Postives = 91/135 (67.41%), Query Frame = 0


Query: 10  VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERLV- 69
           +F + D DGDG++S+ +L        GEE+++EEA  +VA+ D+D DGLL  AEF RLV 
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  -----EDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDV 129
                E+ +  G  ++EAF MYEMEG G IT  SLRRML +LG  + I DC  MI +FD+
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 NGDGVLCFDEFRIMM 139
           NGDGVL FDEF+IMM
Sbjct: 135 NGDGVLSFDEFKIMM 149

[Close]

BLAST of CmoCh04G021950 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-28
Identity = 68/144 (47.22%), Postives = 94/144 (65.28%), Query Frame = 0


Query: 7   YQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFER 66
           ++RVF S D DGDGK+S+ +L     A  GE++ +EE  A++A  D+D DGLL+  EF R
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  LVEDGDGEGKE-----------IKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCN 126
           LV + + +G E           ++EAF MYEMEG G IT +SL+ MLS+LG    + +C 
Sbjct: 67  LVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 RMIAKFDVNGDGVLCFDEFRIMML 140
            MI +FD+NGDGVL FDEF+ MM+
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMMM 150

[Close]

BLAST of CmoCh04G021950 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-28
Identity = 68/139 (48.92%), Postives = 99/139 (71.22%), Query Frame = 0


Query: 5   SRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEF 64
           + ++RVF++ D D DGK+S+ +L     A  GE+++ EEA A+V++ D+DDDGLL+  EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  ERL---VEDGDGEG--KEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIA 124
            +L   +E GD E   + + EAF+MYEMEG G IT  SL+RMLS+LG  + I +C  MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDVNGDGVLCFDEFRIMM 139
           +FD++GDGV+ F+EF+IMM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

[Close]

BLAST of CmoCh04G021950 vs. ExPASy Swiss-Prot
Match: Q2QYW1 (Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=39947 GN=CML25 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.6e-25
Identity = 72/141 (51.06%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 10  VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFER--- 69
           VF + D DGDGKVS+ +L    AA  GEE++EEEAAA++A  D+D DGLL+  EF R   
Sbjct: 8   VFAAFDKDGDGKVSASELRGCMAAALGEEVSEEEAAAILATADTDGDGLLDHHEFMRLSA 67

Query: 70  ---LVEDGDGEGKEIKEAFKMY-EMEGLGVITAVSLRRMLSQLG---QSRSIGDCNRMIA 129
              L E  +   + ++EAF MY E E   VIT  SLRRML +LG   Q   + +C  MI 
Sbjct: 68  AHQLQEPAEESLRCLREAFDMYAEEEETAVITPASLRRMLRRLGSEHQRLEMEECRAMIC 127

Query: 130 KFDVNGDGVLCFDEFRIMMLL 141
           +FD+NGDGVL FDEFR+MML+
Sbjct: 128 RFDLNGDGVLSFDEFRVMMLM 148

[Close]

BLAST of CmoCh04G021950 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-23
Identity = 61/133 (45.86%), Postives = 90/133 (67.67%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERL 67
           + VF+ +D++ DG++S ++L   F  + GE+L++EEA A V   D+D DG+L+  EF +L
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQL 104

Query: 68  VEDGDGEGK--EIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 127
           ++  D E K  E+K AF++Y  EG   IT  SL+ ML +LG+SR+  DC  MI+ FD+N 
Sbjct: 105 IKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 128 DGVLCFDEFRIMM 139
           DGVL FDEF +MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of CmoCh04G021950 vs. ExPASy TrEMBL
Match: A0A6J1HFW4 (probable calcium-binding protein CML31 OS=Cucurbita moschata OX=3662 GN=LOC111463121 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 5.5e-69
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60
           MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE
Sbjct: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60

Query: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120
           IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK
Sbjct: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRIMMLL 141
           FDVNGDGVLCFDEFRIMMLL
Sbjct: 121 FDVNGDGVLCFDEFRIMMLL 140

[Close]

BLAST of CmoCh04G021950 vs. ExPASy TrEMBL
Match: A0A6J1JHC9 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111487021 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.4e-66
Identity = 135/140 (96.43%), Postives = 137/140 (97.86%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60
           MPQFSRYQRVFNSIDSD DGKVSSQDLLTRFAAVTGE LTEEEAAAVVAAYDSDDDGLLE
Sbjct: 1   MPQFSRYQRVFNSIDSDCDGKVSSQDLLTRFAAVTGEHLTEEEAAAVVAAYDSDDDGLLE 60

Query: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120
           IAEFER VE+GDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLG+SRSIGDCNRMIAK
Sbjct: 61  IAEFERFVEEGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGESRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRIMMLL 141
           FDVNGDGVLCFDEFRIMMLL
Sbjct: 121 FDVNGDGVLCFDEFRIMMLL 140

[Close]

BLAST of CmoCh04G021950 vs. ExPASy TrEMBL
Match: A0A0A0KPM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G571560 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.3e-41
Identity = 97/145 (66.90%), Postives = 116/145 (80.00%), Query Frame = 0


Query: 1   MPQFSRYQR-VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLL 60
           M + S Y   +F +ID+D DGKVS  DLLTR  A+TG+ LTE EAA+ VAA+DSD DGLL
Sbjct: 1   MDKLSPYHTLLFYNIDTDSDGKVSPHDLLTRLPALTGQVLTEAEAASAVAAFDSDGDGLL 60

Query: 61  EIAEFERLVE----DGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCN 120
            + EF RLVE     GD + +E++EAF+MYEMEGLGVIT VSLRRMLS+LG+SRS+GDC 
Sbjct: 61  GMEEFGRLVEGVVVGGDDDDREVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCK 120

Query: 121 RMIAKFDVNGDGVLCFDEFRIMMLL 141
           RMIAKFD++GDGVLCFDEFR MMLL
Sbjct: 121 RMIAKFDLDGDGVLCFDEFRGMMLL 145

[Close]

BLAST of CmoCh04G021950 vs. ExPASy TrEMBL
Match: A0A5D3E2X7 (Putative calcium-binding protein CML31 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00150 PE=4 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.2e-41
Identity = 96/144 (66.67%), Postives = 115/144 (79.86%), Query Frame = 0


Query: 1   MPQFSRYQR-VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLL 60
           M + S Y   +F +ID+D DGKVS  DL TR  A+TG +LTE EAA+ VAAYDSD DGLL
Sbjct: 1   MDKLSPYHTLLFYTIDTDSDGKVSPHDLFTRLPALTGLDLTEAEAASAVAAYDSDGDGLL 60

Query: 61  EIAEFERL---VEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNR 120
            + EF RL   V+D D + +E++EAF+MYEMEGLGVIT VSLRRMLS+LG+SRS+GDC R
Sbjct: 61  GMEEFGRLVKGVDDNDDDDREVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCKR 120

Query: 121 MIAKFDVNGDGVLCFDEFRIMMLL 141
           MIAKFD++GDGVLCFDEFR MMLL
Sbjct: 121 MIAKFDLDGDGVLCFDEFRGMMLL 144

[Close]

BLAST of CmoCh04G021950 vs. ExPASy TrEMBL
Match: A0A1S3AUA4 (probable calcium-binding protein CML31 OS=Cucumis melo OX=3656 GN=LOC103483041 PE=4 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.2e-41
Identity = 96/144 (66.67%), Postives = 115/144 (79.86%), Query Frame = 0


Query: 1   MPQFSRYQR-VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLL 60
           M + S Y   +F +ID+D DGKVS  DL TR  A+TG +LTE EAA+ VAAYDSD DGLL
Sbjct: 1   MDKLSPYHTLLFYTIDTDSDGKVSPHDLFTRLPALTGLDLTEAEAASAVAAYDSDGDGLL 60

Query: 61  EIAEFERL---VEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNR 120
            + EF RL   V+D D + +E++EAF+MYEMEGLGVIT VSLRRMLS+LG+SRS+GDC R
Sbjct: 61  GMEEFGRLVKGVDDNDDDDREVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCKR 120

Query: 121 MIAKFDVNGDGVLCFDEFRIMMLL 141
           MIAKFD++GDGVLCFDEFR MMLL
Sbjct: 121 MIAKFDLDGDGVLCFDEFRGMMLL 144

[Close]

BLAST of CmoCh04G021950 vs. NCBI nr
Match: XP_022962715.1 (probable calcium-binding protein CML31 [Cucurbita moschata] >KAG6601838.1 putative calcium-binding protein CML31, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032546.1 putative calcium-binding protein CML31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 270.0 bits (689), Expect = 1.1e-68
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60
           MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE
Sbjct: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60

Query: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120
           IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK
Sbjct: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRIMMLL 141
           FDVNGDGVLCFDEFRIMMLL
Sbjct: 121 FDVNGDGVLCFDEFRIMMLL 140

[Close]

BLAST of CmoCh04G021950 vs. NCBI nr
Match: XP_022989992.1 (putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 260.4 bits (664), Expect = 9.0e-66
Identity = 135/140 (96.43%), Postives = 137/140 (97.86%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60
           MPQFSRYQRVFNSIDSD DGKVSSQDLLTRFAAVTGE LTEEEAAAVVAAYDSDDDGLLE
Sbjct: 1   MPQFSRYQRVFNSIDSDCDGKVSSQDLLTRFAAVTGEHLTEEEAAAVVAAYDSDDDGLLE 60

Query: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120
           IAEFER VE+GDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLG+SRSIGDCNRMIAK
Sbjct: 61  IAEFERFVEEGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGESRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRIMMLL 141
           FDVNGDGVLCFDEFRIMMLL
Sbjct: 121 FDVNGDGVLCFDEFRIMMLL 140

[Close]

BLAST of CmoCh04G021950 vs. NCBI nr
Match: XP_023520938.1 (probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 257.7 bits (657), Expect = 5.8e-65
Identity = 132/137 (96.35%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60
           MPQFSRY+RVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE
Sbjct: 1   MPQFSRYERVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60

Query: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120
           IAEFER VE+GDGEGKEIKEAFKMYEMEGLGVITAVSLRRML QLG+SRSIGDCNRMIAK
Sbjct: 61  IAEFERFVEEGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLIQLGESRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRIM 138
           FDVNGDGVLCFDEFRIM
Sbjct: 121 FDVNGDGVLCFDEFRIM 137

[Close]

BLAST of CmoCh04G021950 vs. NCBI nr
Match: XP_038875509.1 (putative calcium-binding protein CML23 [Benincasa hispida])

HSP 1 Score: 180.3 bits (456), Expect = 1.2e-41
Identity = 94/135 (69.63%), Postives = 113/135 (83.70%), Query Frame = 0


Query: 6   RYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFE 65
           ++  +FN+ID+D DGKVS  DLLTR   +TGE+LTE +AAA VAA+D + DGLL++ EF 
Sbjct: 3   KFTLLFNTIDTDCDGKVSPHDLLTRLPPLTGEDLTEVDAAAAVAAFDWNGDGLLDMEEFG 62

Query: 66  RLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 125
           RLVE  D E  E++EAF+MYEMEGLGVIT VSLRRMLS+LG+SRS+GDC +MIAKFDVNG
Sbjct: 63  RLVEGDDEE--EVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCKKMIAKFDVNG 122

Query: 126 DGVLCFDEFRIMMLL 141
           DGVLCFDEFR MMLL
Sbjct: 123 DGVLCFDEFRTMMLL 135

[Close]

BLAST of CmoCh04G021950 vs. NCBI nr
Match: XP_004150479.1 (probable calcium-binding protein CML31 [Cucumis sativus] >KGN51513.1 hypothetical protein Csa_008833 [Cucumis sativus])

HSP 1 Score: 179.1 bits (453), Expect = 2.6e-41
Identity = 97/145 (66.90%), Postives = 116/145 (80.00%), Query Frame = 0


Query: 1   MPQFSRYQR-VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLL 60
           M + S Y   +F +ID+D DGKVS  DLLTR  A+TG+ LTE EAA+ VAA+DSD DGLL
Sbjct: 1   MDKLSPYHTLLFYNIDTDSDGKVSPHDLLTRLPALTGQVLTEAEAASAVAAFDSDGDGLL 60

Query: 61  EIAEFERLVE----DGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCN 120
            + EF RLVE     GD + +E++EAF+MYEMEGLGVIT VSLRRMLS+LG+SRS+GDC 
Sbjct: 61  GMEEFGRLVEGVVVGGDDDDREVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCK 120

Query: 121 RMIAKFDVNGDGVLCFDEFRIMMLL 141
           RMIAKFD++GDGVLCFDEFR MMLL
Sbjct: 121 RMIAKFDLDGDGVLCFDEFRGMMLL 145

[Close]

BLAST of CmoCh04G021950 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-24
Identity = 61/133 (45.86%), Postives = 90/133 (67.67%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERL 67
           + VF+ +D++ DG++S ++L   F  + GE+L++EEA A V   D+D DG+L+  EF +L
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQL 104

Query: 68  VEDGDGEGK--EIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 127
           ++  D E K  E+K AF++Y  EG   IT  SL+ ML +LG+SR+  DC  MI+ FD+N 
Sbjct: 105 IKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 128 DGVLCFDEFRIMM 139
           DGVL FDEF +MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of CmoCh04G021950 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-24
Identity = 61/133 (45.86%), Postives = 90/133 (67.67%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERL 67
           + VF+ +D++ DG++S ++L   F  + GE+L++EEA A V   D+D DG+L+  EF +L
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQL 104

Query: 68  VEDGDGEGK--EIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 127
           ++  D E K  E+K AF++Y  EG   IT  SL+ ML +LG+SR+  DC  MI+ FD+N 
Sbjct: 105 IKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 128 DGVLCFDEFRIMM 139
           DGVL FDEF +MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of CmoCh04G021950 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 103.2 bits (256), Expect = 1.7e-22
Identity = 59/136 (43.38%), Postives = 88/136 (64.71%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERL 67
           + VF  +D++ DG++S+++L   F  + GE++++EEA A V   D D DG+L+I EF  L
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTL-GEQMSDEEAEAAVKLSDIDGDGMLDINEFALL 83

Query: 68  VEDGD-----GEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFD 127
           ++  D      + ++I EAF+MY  +G   IT  SL+ ML +LG+SR+  DC  MI  FD
Sbjct: 84  IKGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 128 VNGDGVLCFDEFRIMM 139
           +N DGVL FDEF +MM
Sbjct: 144 LNADGVLSFDEFALMM 158

[Close]

BLAST of CmoCh04G021950 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 102.8 bits (255), Expect = 2.2e-22
Identity = 62/133 (46.62%), Postives = 83/133 (62.41%), Query Frame = 0


Query: 10  VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERLVE 69
           VF+ +D++ DGK+S ++L +   ++ G  L+  E   VV   D D DG ++  EF +L+E
Sbjct: 53  VFDYMDANSDGKISGEELQS-CVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLME 112

Query: 70  DGDGEG----KEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 129
             DG      KE+KEAF MY MEG   ITA SLRR LS+LG+S ++  C  MI  FD N 
Sbjct: 113 GEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQND 172

Query: 130 DGVLCFDEFRIMM 139
           DGVL FDEF +MM
Sbjct: 173 DGVLSFDEFVLMM 184

[Close]

BLAST of CmoCh04G021950 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 95.9 bits (237), Expect = 2.7e-20
Identity = 61/140 (43.57%), Postives = 90/140 (64.29%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERL 67
           ++VF+  DSDGDGK+S+ +L   F +V GE ++ E A   +   D+D DG L   +F  L
Sbjct: 66  RQVFSHFDSDGDGKISAFELRHYFGSV-GEYISHEAAQEAINEVDTDADGSLGFEDFVGL 125

Query: 68  VE------DG--DGEGKEIKEAFKMYEME-GLGVITAVSLRRMLSQLGQSRSIGDCNRMI 127
           +       DG  DG+G E+K AF+M+E+E G G IT   L++ML +LG+SR+ G+C  MI
Sbjct: 126 MTRRDLYGDGEVDGDG-ELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMI 185

Query: 128 AKFDVNGDGVLCFDEFRIMM 139
             +D++G+G+L F EFR MM
Sbjct: 186 KFYDIDGNGILDFHEFRQMM 203

The following BLAST results are available for this feature:

BLAST of CmoCh04G021950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYK0	5.4e-29	51.85	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYJ9	1.2e-28	47.22	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYJ8	2.0e-28	48.92	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q2QYW1	1.6e-25	51.06	Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q9SRE6	1.5e-23	45.86	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]

BLAST of CmoCh04G021950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HFW4	5.5e-69	100.00	probable calcium-binding protein CML31 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1JHC9	4.4e-66	96.43	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114870...	[more]
A0A0A0KPM3	1.3e-41	66.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G571560 PE=4 SV=1	[more]
A0A5D3E2X7	2.2e-41	66.67	Putative calcium-binding protein CML31 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3AUA4	2.2e-41	66.67	probable calcium-binding protein CML31 OS=Cucumis melo OX=3656 GN=LOC103483041 P...	[more]

BLAST of CmoCh04G021950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022962715.1	1.1e-68	100.00	probable calcium-binding protein CML31 [Cucurbita moschata] >KAG6601838.1 putati...	[more]
XP_022989992.1	9.0e-66	96.43	putative calcium-binding protein CML19 [Cucurbita maxima]	[more]
XP_023520938.1	5.8e-65	96.35	probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo]	[more]
XP_038875509.1	1.2e-41	69.63	putative calcium-binding protein CML23 [Benincasa hispida]	[more]
XP_004150479.1	2.6e-41	66.90	probable calcium-binding protein CML31 [Cucumis sativus] >KGN51513.1 hypothetica...	[more]

BLAST of CmoCh04G021950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76650.1	1.1e-24	45.86	calmodulin-like 38	[more]
AT1G76650.3	1.1e-24	45.86	calmodulin-like 38	[more]
AT1G76640.1	1.7e-22	43.38	Calcium-binding EF-hand family protein	[more]
AT5G42380.1	2.2e-22	46.62	calmodulin like 37	[more]
AT3G50770.1	2.7e-20	43.57	calmodulin-like 41	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 43..71 e-value: 68.0 score: 3.0 coord: 113..140 e-value: 0.4 score: 19.7 coord: 6..34 e-value: 0.13 score: 21.4 coord: 77..105 e-value: 3.9 score: 13.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 5..68 e-value: 7.2E-11 score: 42.4
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 114..138 e-value: 8.4E-7 score: 28.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 39..74 score: 9.99435
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 109..140 score: 10.747526
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 2..37 score: 10.384886
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 77..138 e-value: 4.42004E-11 score: 52.9353
None	No IPR available	GENE3D	1.10.238.10		coord: 72..140 e-value: 4.8E-15 score: 57.5
None	No IPR available	GENE3D	1.10.238.10		coord: 2..71 e-value: 5.4E-13 score: 50.7
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 4..139
None	No IPR available	PANTHER	PTHR10891:SF858	EF HAND CALCIUM-BINDING FAMILY PROTEIN	coord: 4..139
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 15..27
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 122..134
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 52..64
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 3..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G021950.1	CmoCh04G021950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding