CmoCh04G019540 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G019540
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein EI24 homolog isoform X1
LocationCmo_Chr04: 10000409 .. 10005575 (-)
RNA-Seq ExpressionCmoCh04G019540
SyntenyCmoCh04G019540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCGAACCATAGTCAATCTCGAGTTCTCGACCATCTTCGACCTTGAGGGAGCAGTGATCGAATTGGCGCTCTCCCTCCCCAAAACATTTTGGTGTTCCTGATTTTCTCTGCATTTCAACGATGGATTCTGCTGAACCACTGATTAAGGATCTTGGAGAAAAAGCAAGAAGGGCTTCTCAACTATGGCTTGAAGGTTTTATTGACGCCTGTTGTCTTCACCGTGTTGTGATCCTCTGTCGCAGGTGCCATCTCACGTCTTCCACTTGTACTTTAACTTCGTAGTTTGATTTTCTATCGCTGAGATTACTGGACGGGAATCTATGAACACTAATTCTTACGTTTTTTCCCCCTTTTTTGCTTTTAATTGAGTTTAACAAGCTTTCTCAAGGATGTATGAGGGGAAAGAAGAAGGATTTTCTCGACTTCTTTACTATGAATTAAGCTTCTTTTGTTGTTGTTTTTATAATATCATCTGAGGATGTGTTTCAGGTCGAAAAAGCTTTTGATCCGGACGGGTCAGTGTTTTTTATTGAATGGTTTGATCTTCTTAGGAAGGTAATTCTACTCGACCCTCTCTCTCTCTCTCTTTGCGCATCATTTTCGGGATATAGCATTTACTCTTGTTCCCACTTTTCCGTTTCAAATAAACAACCAATTTGCCTTTTTTTTGTCGGCTCTTCTGTTGTGTTTCTGAATTACGCTCTTTAAGAACTTATTAACTAGTAATATGTTGCAAGGAATTCAAATAGGATAGCACGATCTAGTCTATGTTAGGAATATGCATTCTTTCCATCTCCACATTGGGATGAAGCTATCCAATTTGAACTTAATTCAATTTAGGAATTTATAAACTCACAATATTTCTTCCTTTGATTGATATGGAAATTTGCCTGCATCTTTGTCAATCTATTCATATATTGTCTAAAAATTTAATAGGGTTTCGGTTTTATGACGTTGCGGATCATACGTCTCAAAATCATTGAATTTGAACATTTCTCACGATAATGTCAAAAAATAACAACCGTAACTCAGTTTTTTATTCTGCATGCTTTAACTCAGTCTTGGAGCTAATGAAAACTAAGTTTTGGCTCTTGAGCAGTGGTCTAATCGAACTGAGTCAGAAACTTACTTCTCGACTCTTTCCTTAAAGTTTCTCTCCCTCCTTTTCTCTCTTGAGGGTGAGGGGGATGTCCACATTCTTGTGTCTTGTGGACTACGGTTTAGTTTTTTGTAATAGAAATTAATAACATATTTCAAGGACCTTAATTTTCTCAGAGATTGTTTTGACATATCTAGTTTTCATCAGCACTTCCCTGTCTCTTTTGTTTCTTAACAAAAAAATGTTAAACAATGAAAAGTCAGCAGCTTTCTTGCTTCCTTTTTTGCAGTCTATTGATCCTAAACTCAGTTGTCATCCCAACCCTACAATGGATATTACCGGATCAGCAATCAATGATTGGTTCTCACAAGTTGGCCGCATTTGGTGGTGCTTTAAAATTATATTCTTTCTTACGTCATGGACTGATACAACTTTTTTATGTAAGTACATATTTGATGCCATGTTTTTACTTGTGGGGTTAATAAAGAAGTTGTTTCTCCGGCATGCATCACAAATTATTTAATTTTTCCAACAGTTTCTGTCAAAATTAATTGGAAAAAGACGAACCACGTTTAAAATTACGCTTATAAACTTTTCTTTTTGCGAGTTCAGTAATATTCTTATGGATTATAATATTTCCGTTTGACAGGAGAATTTCTTTGATATTTCAATATGGTTGCATACTATGATTATTGTACTTAACTTGTTTATATGCAATGACTGAACATGATTCAATCAAGAACCTTGGATAACATATTTATTTATTTTGTGTTCATCTCAATTTTTTTTTACCGACTAATTGTTTATGTGAATATTGCTTGCAGTTGTTCTGGTTTTATCCAATGTACATTTTCAGCTTCATTCTGAGCAATATCTGGTATTTCTTTCCCTTCTATTTTCATTCATACTCACACGTTTCTTTTATCATTTCTGCCTCAAATTTGATTTAGTAGGTTTCTAATGCTTATGAATTCTTCAAGCCACATAGTTTCATGATTAAGATTTTATTCAGTTAACGCTTACATTTAAATCTCTGCGTACATCTGGTACACTCATACATCCAGATGGAACGGGTTCAACTAGTAGTTTTTGCGAAGTGACCATTTGTTGATCTGAAGTTTCTGGAGAGTTTCTTTCTGGTCCAGGTACAATGATATTGCTAAACATGGCTTTGCTGCAATGGGACAATCTGAGCCAACCGCAGTGGACTCCTCAGGACTAAACGATGGATCTTCCAATGCTGCTCAAACAGGAAAATCAATTGGATTGGGGAGGTACGTTTTTAATTTGGTCATTGGTGATTTCTGAAATGTTCTTTGCTGCTTGTTTGATGCACTTTTTTGCTTGATACTTGTTTAACCTAAAGTTTTTCTCAGGGTTGTAATAGGAATAGGAGAGCAGCTATATTCTCTACTTCTGATCAACTCCTTCTTCCTTGAGGTAATTGCTTTGAAATTTTGTCTTTCCTTGATGCTCTTATATTTGTTTCTTTCTACTTCATTTGGTTGTGTGTGGTTATCTTTCTTTTATTATGAGCATTTGTAGAAGCCGAGTTGCTTATTTGCAGTTATATATAACATTCACATTCTTCTTCTCGTATCCAATGTCTAAATTGTTTTCTGATACATGTAGGTCTATGCTACAGGTTTCTTGCCTTTCGTGGGAAAGACACTCAATTTTATGCTTCTTTCTTGGATGTACGCCTACTATTGTTTTGAGTATGTGGTGCTTTCCCTTTTAACTTATGATGTGCATCGACTATTCACTTGGTGAACTATTCAATTCAATTCTTTAACTTGTAGGTACAAGTGGAATCTTTCAGAAGTGAGTCTGGATAGAAGGCTGGACTTTTTCGAGTCTAACTGGGCTTTCTTTGCTGGTTTTGGTAGGACTCCATGACTTGATTGATTTATTTAGTTTTAGTTGCTATAGTTTGCTAGTTCCGGTTCTGAATATTAGCAAGGAAAATCTGCTGTTAAATAGTCCATTGCAGCTCCATATGATGTGAAGGTTGATTATAAAGATTAATTTGTTTGTTTTTCAGTGAGATGGCTATTTAGTTAGTTTGATAGGAGAAGTACTAAGTTTGTTTGTTATGATTGGAATATTTTAAAGCGACGGATCTAGGATATAGCTCAATTGGTTAAGGCATAAGGTTAAATGTTTGAATCCTCACATCCACATTCTTGAACTAAGAAAAAGAATCATTACAAATTGGTGGTGGGGAGGGTGAGAGAGGAGTTAAGAGTTCTGATATCAAGCTTGGCTCAAGGAGCCAGTTCTCTATTTACCTGAGATTTTATTCGTATGTTACCATCATTTCCGTACAATAAAAGGTTTCTTTATTCTTGGCTTCTCTCGCTCCCTAAGTTAAGACAACCACCATTATAGCTCTCAATTGGCATATATTGGCTCTTGGCAGTTAACATTAACTTCTTGGTAGACAAAATTGTTACAAATGTGAGCAGTGGATCTAGAAGCAAATATTGAGTATTTGATGGTGTTAAATCCTTTCTCATGACATTTTGCTTTCATTTCATATTTTAGAGTTTTATTTTTATTTCGTGTTTGACTTAAGGCTCATGTCTTTAATTATTAATTAAAATTTGAGCTCATTTCTCTTTTCTTGTCATGGTCACCATAGTTGTGATTTGAAACAATACGTTATACTTTTAAGGAAGTTTGAGGCATACCAGTTTAATGGTCTTTCCAGGTAGTCCTTGCGTTCTTGCGATATTTTTCTTCTCACCACTTGTGAGCTATGGAGTAATGGCTATCTTATTTCCACTGGTAAATCGAACCATTTGTCTCCCTCTCTTCACACACACAAACTCATTTCCTTCTAAATTTGTACCGATGTTCTCTTTCCTCAAATACTCACGTCTTACGGCTCTTTTGTTTGGGTTTCATCCCATATGCCTTTTAGTTTGTTCTGACAGCCACGGGCTCAGTTGCTGAGAAGGATATTTATTCACAGAGAACAACTTGGAAATGTGCAGGACTGGGAAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGTAAGTTGTCTTTCTTCACGCTAAACTTGTATTTGCAAAATAGAATAATTTGCTGAGACTATTCGAATAACCTTTGGATTTCTGATCTAATAATAATTTCTTGTATCTATATAGAATAGAAGTTGACTAAATGTGACAAGGAACGATGTTTTAAATGTTAAGCAAATGGTGCAAAATCTCTCTGGAGTTGTAACGGGGAAAACAGCAGTCTTTCTAAAAAATGGACAAAAGAAAGAAATTCATCTTCACGAAGATAATAGATAGAGGTTTATTTAGTGACTTTGTGCTCTGTAATATAGGAGTAGAATAAATTGTAGAGATTGAACCAACGAATGGAAAACCTGAAAAACTTCAATTATGTGATATTTTGAACTTTACTAGATTAGAACTGTCCGTGAAGCAGGGAAACTTTAGTATTGCCTACCAATGTTTTTTCCCCATGTTGATTGGCCTTGAAGGATGAAGCTCTTGACTCTCTTCCCTCATGAATCACAAGAAAAGATGCAGCAAAACAAGGAACTATAACAGGTTCTGGAGTCTGAATCAATACATATCCGTTTCATGAACAGTAAGCCAAAATTCATTAGCCATTGCCTTCTATTCAGAATATTTCCTGCATGTTCATTTATTTATGAAGTATTGCTGCATAATATATCGTCTCTCAATTCTTAATCTATCTGATGGAGCAGAATCCAAGTGGCTCAAGTCAGATGCTACAAGTGATTCCTACCTCATCCGTACAGAAACGATACCGTAGGTGAAGATCAACCATAGGTAGATTCATAACCGCAGTTTGTAAGATTGGTTGCTGTGTGAATATCGAATAGGTACGCAATTAATTGGGCGAATAGATAAGAATGTGCGATGCCGAGTGATAATTTTTCTGTAACCTCGTTAAATAATGTAAATAACTTATGTTTAGTAGAAAGATTGTTCCAACTCTCTGCTTCAGTTCTTTGTGTGAATTATGTACAATCAAGAATGAACAACTTAGAGTGGATAGAGTTGATAAAATCGTTGTGCA

mRNA sequence

GAAGCGAACCATAGTCAATCTCGAGTTCTCGACCATCTTCGACCTTGAGGGAGCAGTGATCGAATTGGCGCTCTCCCTCCCCAAAACATTTTGGTGTTCCTGATTTTCTCTGCATTTCAACGATGGATTCTGCTGAACCACTGATTAAGGATCTTGGAGAAAAAGCAAGAAGGGCTTCTCAACTATGGCTTGAAGGTTTTATTGACGCCTGTTGTCTTCACCGTGTTGTGATCCTCTGTCGCAGGTCGAAAAAGCTTTTGATCCGGACGGGTCAGTGTTTTTTATTGAATGGTTTGATCTTCTTAGGAAGTCTATTGATCCTAAACTCAGTTGTCATCCCAACCCTACAATGGATATTACCGGATCAGCAATCAATGATTGGTTCTCACAAGTTGGCCGCATTTGGTGGTGCTTTAAAATTATATTCTTTCTTACGTCATGGACTGATACAACTTTTTTATTTGTTCTGGTTTTATCCAATGTACATTTTCAGCTTCATTCTGAGCAATATCTGGTACAATGATATTGCTAAACATGGCTTTGCTGCAATGGGACAATCTGAGCCAACCGCAGTGGACTCCTCAGGACTAAACGATGGATCTTCCAATGCTGCTCAAACAGGAAAATCAATTGGATTGGGGAGGGTTGTAATAGGAATAGGAGAGCAGCTATATTCTCTACTTCTGATCAACTCCTTCTTCCTTGAGGTCTATGCTACAGGTTTCTTGCCTTTCGTGGGAAAGACACTCAATTTTATGCTTCTTTCTTGGATGTACGCCTACTATTGTTTTGAGTACAAGTGGAATCTTTCAGAAGTGAGTCTGGATAGAAGGCTGGACTTTTTCGAGTCTAACTGGGCTTTCTTTGCTGGTTTTGGTAGTCCTTGCGTTCTTGCGATATTTTTCTTCTCACCACTTGTGAGCTATGGAGTAATGGCTATCTTATTTCCACTGTTTGTTCTGACAGCCACGGGCTCAGTTGCTGAGAAGGATATTTATTCACAGAGAACAACTTGGAAATGTGCAGGACTGGGAAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGATGAAGCTCTTGACTCTCTTCCCTCATGAATCACAAGAAAAGATGCAGCAAAACAAGGAACTATAACAGGTTCTGGAGTCTGAATCAATACATATCCGTTTCATGAACAAATCCAAGTGGCTCAAGTCAGATGCTACAAGTGATTCCTACCTCATCCGTACAGAAACGATACCGTAGGTGAAGATCAACCATAGGTAGATTCATAACCGCAGTTTGTAAGATTGGTTGCTGTGTGAATATCGAATAGGTACGCAATTAATTGGGCGAATAGATAAGAATGTGCGATGCCGAGTGATAATTTTTCTGTAACCTCGTTAAATAATGTAAATAACTTATGTTTAGTAGAAAGATTGTTCCAACTCTCTGCTTCAGTTCTTTGTGTGAATTATGTACAATCAAGAATGAACAACTTAGAGTGGATAGAGTTGATAAAATCGTTGTGCA

Coding sequence (CDS)

ATGGATTCTGCTGAACCACTGATTAAGGATCTTGGAGAAAAAGCAAGAAGGGCTTCTCAACTATGGCTTGAAGGTTTTATTGACGCCTGTTGTCTTCACCGTGTTGTGATCCTCTGTCGCAGGTCGAAAAAGCTTTTGATCCGGACGGGTCAGTGTTTTTTATTGAATGGTTTGATCTTCTTAGGAAGTCTATTGATCCTAAACTCAGTTGTCATCCCAACCCTACAATGGATATTACCGGATCAGCAATCAATGATTGGTTCTCACAAGTTGGCCGCATTTGGTGGTGCTTTAAAATTATATTCTTTCTTACGTCATGGACTGATACAACTTTTTTATTTGTTCTGGTTTTATCCAATGTACATTTTCAGCTTCATTCTGAGCAATATCTGGTACAATGATATTGCTAAACATGGCTTTGCTGCAATGGGACAATCTGAGCCAACCGCAGTGGACTCCTCAGGACTAAACGATGGATCTTCCAATGCTGCTCAAACAGGAAAATCAATTGGATTGGGGAGGGTTGTAATAGGAATAGGAGAGCAGCTATATTCTCTACTTCTGATCAACTCCTTCTTCCTTGAGGTCTATGCTACAGGTTTCTTGCCTTTCGTGGGAAAGACACTCAATTTTATGCTTCTTTCTTGGATGTACGCCTACTATTGTTTTGAGTACAAGTGGAATCTTTCAGAAGTGAGTCTGGATAGAAGGCTGGACTTTTTCGAGTCTAACTGGGCTTTCTTTGCTGGTTTTGGTAGTCCTTGCGTTCTTGCGATATTTTTCTTCTCACCACTTGTGAGCTATGGAGTAATGGCTATCTTATTTCCACTGTTTGTTCTGACAGCCACGGGCTCAGTTGCTGAGAAGGATATTTATTCACAGAGAACAACTTGGAAATGTGCAGGACTGGGAAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGATGAAGCTCTTGACTCTCTTCCCTCATGAATCACAAGAAAAGATGCAGCAAAACAAGGAACTATAA

Protein sequence

MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDFFESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL
Homology
BLAST of CmoCh04G019540 vs. ExPASy Swiss-Prot
Match: Q5BPL5 (Protein EI24 homolog OS=Arabidopsis thaliana OX=3702 GN=At4g06676 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 1.9e-109
Identity = 206/320 (64.38%), Postives = 249/320 (77.81%), Query Frame = 0

Query: 14  KARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVVIP 73
           K+++   LWLEGF +AC LHRVVILC RS+KLL+RTGQCFLLNGLIFLGSL +    + P
Sbjct: 4   KSKQVLLLWLEGFREACSLHRVVILCLRSRKLLLRTGQCFLLNGLIFLGSLGVFKWFIDP 63

Query: 74  TLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPMYIFSFILSNIWYN 133
           +LQWILPDQ S + S +  ++GG    Y+FLR GL+QLFY+FWFYP+Y+ SFILSNIWYN
Sbjct: 64  SLQWILPDQCSPLTSQEFCSYGG---FYAFLRGGLLQLFYVFWFYPLYMLSFILSNIWYN 123

Query: 134 DIAKHGFAAMGQSEPTAVDS--SGLNDGSSNAAQTGKSIGLGRVVIGIGEQLYSLLLINS 193
           DIAKHGF A+  S+  + ++   G    S N A   +  GLG V+IGIGEQ+YS+LL+  
Sbjct: 124 DIAKHGFEAIEISDLNSAEALRQGEALASLNMANAERPSGLGGVMIGIGEQVYSILLLTF 183

Query: 194 FFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDFFESNWAFFAGF 253
           FFLEV   G +P++GK LNF+LLSWMYAYYC+EYKWN S +SL +RLDFF+SNWAFFAGF
Sbjct: 184 FFLEVCVVGVIPYIGKILNFLLLSWMYAYYCYEYKWNFSGISLKKRLDFFQSNWAFFAGF 243

Query: 254 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 313
           GSPCVLAIFF SPLVS  +MAILFPLFVLTATGS  EK I + R TWKCAGLG+LPIFY 
Sbjct: 244 GSPCVLAIFFLSPLVSGALMAILFPLFVLTATGSGPEKLIGAPRRTWKCAGLGKLPIFYI 303

Query: 314 ANTLSMKLLTLF----PHES 328
           A+TLSM  L++F    PHE+
Sbjct: 304 ADTLSMLALSIFRLESPHEN 320

BLAST of CmoCh04G019540 vs. ExPASy Swiss-Prot
Match: Q54PW9 (Protein EI24 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0284253 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 9.0e-22
Identity = 84/324 (25.93%), Postives = 153/324 (47.22%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           +D+  P +K++         +WL G  D+  L   +I   +S+ L      C  LNG+IF
Sbjct: 12  IDNTIPQVKEM------FKLIWL-GVADSMKLKGAIIRTIKSEVLRKNFIHCIFLNGIIF 71

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LG+ LI    V P L ++L        +H           +  L +    +++  W YP+
Sbjct: 72  LGTYLIYLYWVSPMLNYLL--------NH-----------FPTLSNMFTIIYFSLWVYPV 131

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFS I ++ WY +IAK  F   G++  T  +S+                    ++    
Sbjct: 132 YIFSIIANSKWYTEIAKESFVISGRT--TFANST------------------NGILSSFV 191

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNL-SEVSLDRRLD 240
           +++Y  LL     +      F+P+    +NF++++W+Y+++CF+YKW L  + +L +R+ 
Sbjct: 192 DEIYRNLLFGVILVMSAIIAFIPYT-NFINFVIITWLYSFWCFDYKWILRGKWNLLQRIQ 251

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWK 300
           +FE++WA+  G+G       FFF  L+   + +IL+PLF++ +  +   K +       K
Sbjct: 252 YFETHWAYMFGYGLIFTTCSFFFPMLIGNAIFSILYPLFIILSISAKPTKMVNQDGILPK 283

Query: 301 CAGLGRLPIFYAANTLSMKLLTLF 324
                ++PIFY    +   +L L+
Sbjct: 312 -----QIPIFYVPEIIVNVILKLY 283

BLAST of CmoCh04G019540 vs. ExPASy Swiss-Prot
Match: Q08DE5 (Etoposide-induced protein 2.4 homolog OS=Bos taurus OX=9913 GN=EI24 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.8e-15
Identity = 70/242 (28.93%), Postives = 113/242 (46.69%), Query Frame = 0

Query: 44  KLLIRTGQCFLLNGLIFLGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSF 103
           +++ R  QC   NG +F  SLL+   V IP LQ +      +IG   L        ++S+
Sbjct: 66  RIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSV---TAQIIGDPSLHG-----DVWSW 125

Query: 104 LRHGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNA 163
           L   L  +F   W  P+++ S +++ IW+ DIA   F   G+        S +       
Sbjct: 126 LEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAFEVSGRKPHPFPSVSKI------- 185

Query: 164 AQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATGFLP--FVGKTLNFMLLSWMYAYY 223
                          I + L++LLL   F L+       P   VG+ ++ + +S +Y+ Y
Sbjct: 186 ---------------IADMLFNLLLQALFLLQGMFVSLFPIHLVGQLVSLLHMSLLYSLY 245

Query: 224 CFEYKWNLSEVSLDRRLDFFESNWAFFAGFGSP-CVLAIFFFSPLVSYGVMAILFPLFVL 283
           CFEY+W    + + +RL   E NW ++ GFG P   L     S +VS  + +ILFPLF++
Sbjct: 246 CFEYRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIVSGCLFSILFPLFII 277

BLAST of CmoCh04G019540 vs. ExPASy Swiss-Prot
Match: O14681 (Etoposide-induced protein 2.4 homolog OS=Homo sapiens OX=9606 GN=EI24 PE=1 SV=4)

HSP 1 Score: 82.8 bits (203), Expect = 8.1e-15
Identity = 68/242 (28.10%), Postives = 113/242 (46.69%), Query Frame = 0

Query: 44  KLLIRTGQCFLLNGLIFLGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSF 103
           +++ R  QC   NG +F  SLL+   V IP LQ +      +IG   L        ++S+
Sbjct: 66  RIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSV---TARIIGDPSLHG-----DVWSW 125

Query: 104 LRHGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNA 163
           L   L  +F   W  P+++ S +++ IW+ DIA   F   G+        S +       
Sbjct: 126 LEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAFEVSGRKPHPFPSVSKI------- 185

Query: 164 AQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATGFLP--FVGKTLNFMLLSWMYAYY 223
                          I + L++LLL   F ++       P   VG+ ++ + +S +Y+ Y
Sbjct: 186 ---------------IADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSLLYSLY 245

Query: 224 CFEYKWNLSEVSLDRRLDFFESNWAFFAGFGSP-CVLAIFFFSPLVSYGVMAILFPLFVL 283
           CFEY+W    + + +RL   E NW ++ GFG P   L     S ++S  + +ILFPLF++
Sbjct: 246 CFEYRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFII 277

BLAST of CmoCh04G019540 vs. ExPASy Swiss-Prot
Match: Q61070 (Etoposide-induced protein 2.4 OS=Mus musculus OX=10090 GN=Ei24 PE=1 SV=3)

HSP 1 Score: 82.8 bits (203), Expect = 8.1e-15
Identity = 68/242 (28.10%), Postives = 113/242 (46.69%), Query Frame = 0

Query: 44  KLLIRTGQCFLLNGLIFLGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSF 103
           +++ R  QC   NG +F  SLL+   V IP LQ +      +IG   L        ++S+
Sbjct: 66  RIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSV---TARIIGDPSLHG-----DVWSW 125

Query: 104 LRHGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNA 163
           L   L  +F   W  P+++ S +++ IW+ DIA   F   G+        S +       
Sbjct: 126 LEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAFEVSGRKPHPFPSVSKI------- 185

Query: 164 AQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATGFLP--FVGKTLNFMLLSWMYAYY 223
                          I + L++LLL   F ++       P   VG+ ++ + +S +Y+ Y
Sbjct: 186 ---------------IADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSLLYSLY 245

Query: 224 CFEYKWNLSEVSLDRRLDFFESNWAFFAGFGSP-CVLAIFFFSPLVSYGVMAILFPLFVL 283
           CFEY+W    + + +RL   E NW ++ GFG P   L     S ++S  + +ILFPLF++
Sbjct: 246 CFEYRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFII 277

BLAST of CmoCh04G019540 vs. ExPASy TrEMBL
Match: A0A6J1H083 (protein EI24 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.4e-187
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300

Query: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 338
           AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 337

BLAST of CmoCh04G019540 vs. ExPASy TrEMBL
Match: A0A6J1K7C5 (protein EI24 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492932 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 3.6e-183
Identity = 330/337 (97.92%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           M+SAEPLIKD GEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MNSAEPLIKDFGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMIGSHKL AFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLGAFGGALKLYSFLRHGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQS PT VD SGLNDGSSNAAQTGKS GLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSGPTTVDPSGLNDGSSNAAQTGKSNGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300

Query: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 338
           AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 337

BLAST of CmoCh04G019540 vs. ExPASy TrEMBL
Match: A0A6J1H1Y7 (protein EI24 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.6e-175
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300

Query: 301 AGLGRLPIFYAANTLS 317
           AGLGRLPIFYAANTLS
Sbjct: 301 AGLGRLPIFYAANTLS 316

BLAST of CmoCh04G019540 vs. ExPASy TrEMBL
Match: A0A1S3BE01 (protein EI24 homolog OS=Cucumis melo OX=3656 GN=LOC103488854 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 309/337 (91.69%), Postives = 323/337 (95.85%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           MDSAEPLI+ +GEK RRASQLWLEGFIDACCLHR++ILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MDSAEPLIEVVGEKLRRASQLWLEGFIDACCLHRIIILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLL+LNSV+IPTLQWILPDQQS+I SHKL+AFG  LKLYSFLR GLIQLFYLFWFYPM
Sbjct: 61  LGSLLMLNSVIIPTLQWILPDQQSVINSHKLSAFGATLKLYSFLRLGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQSEPTA + S  N+GS N AQTGKSIGLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAANPSRQNEGSFNTAQTGKSIGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLP VGKTLNF+LLSWMYAYYCFEYKWNLS+VSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAE+DIYSQR TWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEQDIYSQRATWKC 300

Query: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 338
           AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNK+L
Sbjct: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKDL 337

BLAST of CmoCh04G019540 vs. ExPASy TrEMBL
Match: A0A6J1KG79 (protein EI24 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492932 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.4e-171
Identity = 309/316 (97.78%), Postives = 310/316 (98.10%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           M+SAEPLIKD GEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MNSAEPLIKDFGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMIGSHKL AFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLGAFGGALKLYSFLRHGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQS PT VD SGLNDGSSNAAQTGKS GLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSGPTTVDPSGLNDGSSNAAQTGKSNGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300

Query: 301 AGLGRLPIFYAANTLS 317
           AGLGRLPIFYAANTLS
Sbjct: 301 AGLGRLPIFYAANTLS 316

BLAST of CmoCh04G019540 vs. NCBI nr
Match: XP_022957442.1 (protein EI24 homolog isoform X1 [Cucurbita moschata] >XP_022957443.1 protein EI24 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 664.5 bits (1713), Expect = 5.0e-187
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300

Query: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 338
           AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 337

BLAST of CmoCh04G019540 vs. NCBI nr
Match: KAG6601621.1 (Protein EI24-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 655.6 bits (1690), Expect = 2.3e-184
Identity = 334/337 (99.11%), Postives = 334/337 (99.11%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           MDSAE LIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 133 MDSAELLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 192

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 193 LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 252

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAK GFAAMGQSEPTAVD SGLNDGSSNAAQTGKSIGLGRVVIGIG
Sbjct: 253 YIFSFILSNIWYNDIAKQGFAAMGQSEPTAVDPSGLNDGSSNAAQTGKSIGLGRVVIGIG 312

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 313 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 372

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 373 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 432

Query: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 338
           AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 433 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 469

BLAST of CmoCh04G019540 vs. NCBI nr
Match: XP_023515037.1 (protein EI24 homolog [Cucurbita pepo subsp. pepo] >XP_023515046.1 protein EI24 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 652.9 bits (1683), Expect = 1.5e-183
Identity = 331/337 (98.22%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           MDSAEPLIKD GEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MDSAEPLIKDFGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMI SHKL AFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 61  LGSLLILNSVVIPTLQWILPDQQSMINSHKLGAFGGALKLYSFLRHGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQS PTAVD SGLNDGSSNAAQTGKSIGLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSGPTAVDPSGLNDGSSNAAQTGKSIGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300

Query: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 338
           AGLGR PIFYAANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 301 AGLGRFPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 337

BLAST of CmoCh04G019540 vs. NCBI nr
Match: XP_022998222.1 (protein EI24 homolog isoform X1 [Cucurbita maxima] >XP_022998232.1 protein EI24 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 650.6 bits (1677), Expect = 7.5e-183
Identity = 330/337 (97.92%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           M+SAEPLIKD GEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MNSAEPLIKDFGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMIGSHKL AFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLGAFGGALKLYSFLRHGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQS PT VD SGLNDGSSNAAQTGKS GLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSGPTTVDPSGLNDGSSNAAQTGKSNGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300

Query: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 338
           AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 301 AGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 337

BLAST of CmoCh04G019540 vs. NCBI nr
Match: XP_022957444.1 (protein EI24 homolog isoform X2 [Cucurbita moschata])

HSP 1 Score: 625.2 bits (1611), Expect = 3.4e-175
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60
           MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF
Sbjct: 1   MDSAEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIF 60

Query: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120
           LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM
Sbjct: 61  LGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPM 120

Query: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180
           YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG
Sbjct: 121 YIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGIG 180

Query: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240
           EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF
Sbjct: 181 EQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDF 240

Query: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300
           FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC
Sbjct: 241 FESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKC 300

Query: 301 AGLGRLPIFYAANTLS 317
           AGLGRLPIFYAANTLS
Sbjct: 301 AGLGRLPIFYAANTLS 316

BLAST of CmoCh04G019540 vs. TAIR 10
Match: AT4G06676.1 (CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 264 Blast hits to 262 proteins in 100 species: Archae - 0; Bacteria - 0; Metazoa - 130; Fungi - 16; Plants - 79; Viruses - 0; Other Eukaryotes - 39 (source: NCBI BLink). )

HSP 1 Score: 397.1 bits (1019), Expect = 1.4e-110
Identity = 206/320 (64.38%), Postives = 249/320 (77.81%), Query Frame = 0

Query: 14  KARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVVIP 73
           K+++   LWLEGF +AC LHRVVILC RS+KLL+RTGQCFLLNGLIFLGSL +    + P
Sbjct: 4   KSKQVLLLWLEGFREACSLHRVVILCLRSRKLLLRTGQCFLLNGLIFLGSLGVFKWFIDP 63

Query: 74  TLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPMYIFSFILSNIWYN 133
           +LQWILPDQ S + S +  ++GG    Y+FLR GL+QLFY+FWFYP+Y+ SFILSNIWYN
Sbjct: 64  SLQWILPDQCSPLTSQEFCSYGG---FYAFLRGGLLQLFYVFWFYPLYMLSFILSNIWYN 123

Query: 134 DIAKHGFAAMGQSEPTAVDS--SGLNDGSSNAAQTGKSIGLGRVVIGIGEQLYSLLLINS 193
           DIAKHGF A+  S+  + ++   G    S N A   +  GLG V+IGIGEQ+YS+LL+  
Sbjct: 124 DIAKHGFEAIEISDLNSAEALRQGEALASLNMANAERPSGLGGVMIGIGEQVYSILLLTF 183

Query: 194 FFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDFFESNWAFFAGF 253
           FFLEV   G +P++GK LNF+LLSWMYAYYC+EYKWN S +SL +RLDFF+SNWAFFAGF
Sbjct: 184 FFLEVCVVGVIPYIGKILNFLLLSWMYAYYCYEYKWNFSGISLKKRLDFFQSNWAFFAGF 243

Query: 254 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 313
           GSPCVLAIFF SPLVS  +MAILFPLFVLTATGS  EK I + R TWKCAGLG+LPIFY 
Sbjct: 244 GSPCVLAIFFLSPLVSGALMAILFPLFVLTATGSGPEKLIGAPRRTWKCAGLGKLPIFYI 303

Query: 314 ANTLSMKLLTLF----PHES 328
           A+TLSM  L++F    PHE+
Sbjct: 304 ADTLSMLALSIFRLESPHEN 320

BLAST of CmoCh04G019540 vs. TAIR 10
Match: AT4G06676.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 261 Blast hits to 259 proteins in 97 species: Archae - 0; Bacteria - 0; Metazoa - 130; Fungi - 16; Plants - 81; Viruses - 0; Other Eukaryotes - 34 (source: NCBI BLink). )

HSP 1 Score: 334.0 bits (855), Expect = 1.4e-91
Identity = 172/268 (64.18%), Postives = 209/268 (77.99%), Query Frame = 0

Query: 14  KARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVVIP 73
           K+++   LWLEGF +AC LHRVVILC RS+KLL+RTGQCFLLNGLIFLGSL +    + P
Sbjct: 4   KSKQVLLLWLEGFREACSLHRVVILCLRSRKLLLRTGQCFLLNGLIFLGSLGVFKWFIDP 63

Query: 74  TLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYPMYIFSFILSNIWYN 133
           +LQWILPDQ S + S +  ++GG    Y+FLR GL+QLFY+FWFYP+Y+ SFILSNIWYN
Sbjct: 64  SLQWILPDQCSPLTSQEFCSYGG---FYAFLRGGLLQLFYVFWFYPLYMLSFILSNIWYN 123

Query: 134 DIAKHGFAAMGQSEPTAVDS--SGLNDGSSNAAQTGKSIGLGRVVIGIGEQLYSLLLINS 193
           DIAKHGF A+  S+  + ++   G    S N A   +  GLG V+IGIGEQ+YS+LL+  
Sbjct: 124 DIAKHGFEAIEISDLNSAEALRQGEALASLNMANAERPSGLGGVMIGIGEQVYSILLLTF 183

Query: 194 FFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDFFESNWAFFAGF 253
           FFLEV   G +P++GK LNF+LLSWMYAYYC+EYKWN S +SL +RLDFF+SNWAFFAGF
Sbjct: 184 FFLEVCVVGVIPYIGKILNFLLLSWMYAYYCYEYKWNFSGISLKKRLDFFQSNWAFFAGF 243

Query: 254 GSPCVLAIFFFSPLVSYGVMAILFPLFV 280
           GSPCVLAIFF SPLVS  +MAILFPL V
Sbjct: 244 GSPCVLAIFFLSPLVSGALMAILFPLNV 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5BPL51.9e-10964.38Protein EI24 homolog OS=Arabidopsis thaliana OX=3702 GN=At4g06676 PE=2 SV=1[more]
Q54PW99.0e-2225.93Protein EI24 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0284253 PE=3 S... [more]
Q08DE52.8e-1528.93Etoposide-induced protein 2.4 homolog OS=Bos taurus OX=9913 GN=EI24 PE=2 SV=1[more]
O146818.1e-1528.10Etoposide-induced protein 2.4 homolog OS=Homo sapiens OX=9606 GN=EI24 PE=1 SV=4[more]
Q610708.1e-1528.10Etoposide-induced protein 2.4 OS=Mus musculus OX=10090 GN=Ei24 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1H0832.4e-187100.00protein EI24 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE... [more]
A0A6J1K7C53.6e-18397.92protein EI24 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492932 PE=4... [more]
A0A6J1H1Y71.6e-175100.00protein EI24 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE... [more]
A0A1S3BE011.3e-17291.69protein EI24 homolog OS=Cucumis melo OX=3656 GN=LOC103488854 PE=4 SV=1[more]
A0A6J1KG792.4e-17197.78protein EI24 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492932 PE=4... [more]
Match NameE-valueIdentityDescription
XP_022957442.15.0e-187100.00protein EI24 homolog isoform X1 [Cucurbita moschata] >XP_022957443.1 protein EI2... [more]
KAG6601621.12.3e-18499.11Protein EI24-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023515037.11.5e-18398.22protein EI24 homolog [Cucurbita pepo subsp. pepo] >XP_023515046.1 protein EI24 h... [more]
XP_022998222.17.5e-18397.92protein EI24 homolog isoform X1 [Cucurbita maxima] >XP_022998232.1 protein EI24 ... [more]
XP_022957444.13.4e-175100.00protein EI24 homolog isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G06676.11.4e-11064.38CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 264 ... [more]
AT4G06676.21.4e-9164.18FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF07264EI24coord: 31..276
e-value: 4.4E-38
score: 131.3
IPR009890Etoposide-induced 2.4PANTHERPTHR21389P53 INDUCED PROTEINcoord: 13..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G019540.1CmoCh04G019540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane