CmoCh04G013340 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G013340
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionFAD synthase
LocationCmo_Chr04: 6806784 .. 6811240 (+)
RNA-Seq ExpressionCmoCh04G013340
SyntenyCmoCh04G013340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAAGTCTGCGCCTCTCATTACTCCCCACTTGCCGCCTCCACTCGGTCCCAAGCGGCTGGCGATGTTGGCTGCCGGCGCTCGCGTTTCTCACCATCTCCGGGAGTGCGACTCTCAATTTGGCTTCGGATTAACCTCAGGCCTTACCTCCTTCCCCGTCGTTCTATTTCCTCCGATATGGTCTCGCAAGCCCACTTCTAATTTCCCTTCAATTTCTCGCCGCTCCCAACCACGTGGGGTTTCTTTCTTTTGCTCCATTATCCCCTCCACTTCCCCCGGAGAAATCCCCGTTATCTCCGATTCTTTTGGGTACTTTCCCATTTCATGTCCTTCGATTTCTTATCGCTGTCCGTGTTGTTTTCTTGCTAACTACAAATTTACCAATTGGCGTTTTGTTTCCCAGTTCACGAGAGGATGATCGTGAAGTCTCTGTGGCTGGTTTGTTTTCCTTCGTTGGTTCAATTTTCTCCTGAATCTGTCGTTAATTTTATGGAAGAATGCTAATCCTTATCTTCGTCTTAATTTTTCCGCTTATTTAATGTGTAGGAAGTTTGTGGTGATTTTGGGTTAGATTGAAGTTGAACAACCTTTTGATTATATGGTTGTGTACTTATAATGTGGGTTCTAAGTAGCTCTGATTTATACGCAATACATTAGAATGGGGGGTTTTTTTCCTCTTAAAGATTCAAGCCCCCAAAAGGGAGTGGGAGCAAAACGTAACTCCCGAAGAAAGAACAAGGCTTCAAATGTTAGAAGAGTTTAAAAATAAAAACATTAATGAACTAAGCGCCTAAGATCTTGACACCATCGATGATCCTAAGATTTAGGCTGTTGATGCGTCTTTGTTCTACTTAATGGTCAAACAGTTGATGTGCTTAGAAAATAATTGATCGGTTAACATAGCAAGTAGGAATGGAATATAATACACTTATGTGATGTCCCACATTGGTTGGGGAGGAGAACAAACCACCATTTATAAGGGTGTGGAAACCTTCCCCTAGCAGATGCGTTTTAAAGCCTTGAGGGGAAGCTCGAAAGGGAAAATCCAAAGAGAACAATATATGCTAGCAGTGGATTTGGATCGTTACACTTACGAAGTCACCAACTTGAGAGCAAGTAGCGAAAGCTTAAATATTGATTATTTTAATAAAAAGAAACCAAGATTTCAATGAAAAAAAATGAAAGGATCCAAAAGAGGCTACAAAATGGACAACCGAACAAAAGGAGCCAAAAGCAAGCTAACCTAAAGTTGGTCAGATGGGTGTCTAGTAGCTTTGATTGATAGTTTTAGGAATGTAAGTCATGTTAGGAATCACGACCCTCCACAATGGTATGATATTGTTCACTTTGAGCATAAGCTGTCATGGCTTTGTTTTGGGTTTTCTCAAAAGGTCTCATACCGATGGAGATGTATTCCTTACTCATAAACCCATGATCATTCCCTAAATTAGTCAAGGTGGGACTCCCTCCTAATAATCCCCAACAAGTCACAGTATTAGATGGATTCTTGTCTTCTATCTCTAGTTACTTCGGTTTGGGTATTATGTGAGAAGGTTTTTTTCCCCTTCTTTTGGATTAGCTTGCTATAAGAAAGTTCATTCAGTTGTGAAAAGATCATTTTCTCCTATTATTTGTAGTTCGGTTAGTCTGAGACTGGTTTTTATGATCATTTTTTTTTTTGTCACTCATAGGAGGAATAGTAGCATTAGGAAAATTTGATGCTCTTCACGTTGGACATCGAGAGCTCGCAATTCAAGCTTCAACGGTTGGATCTCCATTTCTATTGTCATTCGTTGGAATTGCTGAAGTACTCGGTTGGGAACCTAGGTAAGCAAAATGCGTTTTCTTTATGTTACTTATTGTTTGTTTCGTCATAAATGGTTGGAAATTTACATCTGACAATGACCTTACAAAATAAAATTTTATGCAGTTTTGATTGTTTTCTAAGGTTCTTACTTGTTCCATTGGCAAATATATGCAACTACCCGATATTTCCCTGTGGAAGATTTGCCCATTCTAATTGAATTTGGTTCCAACAACATTTGTAACATTAGTAATTCACATTGAGTTTCCAATGGAAGTCTTGCAAGTTATAAAGGTGTAGTTATTATCATAGAACACTAAGTTGATGGCCAGCAAGAATTAGCGTGTGCAGCAACAGCAGAAAGAAAGCAAAGCACTCAACATTATGGTTGGCTGTCAACGATCTGCAGCAAAGAATGGAGGAAAGATTTGAGGTCACTGATCGAGATATTCAATGTAGTTTAACAGCAATTGATCAATTTGTTGTTGAAGGATACAAGTTCCCAAAAGGAAACAAAAATTGAAAGAACTAAAGAAAACAGGAGAGTATAACAAGAAGAACATATAGCTAAAAACAAGCAAGAACAGTATCTTCAAAATGACAAACTAAAGCTCTTTCAATTGGAATGTCTGGCTGTCACCATGTGAGAATCAGGACGAGATTTTGAATACCATCTGTTATATTAACTGAGGATTTGTAATGAAGGTGTTGAGTTTATTCAATTGCAGATCTCTAATCTCTTTCCTTTGTTCTGAACATGTACTTAGAATTATATTCTTGGAGTTAATTATTTATCTGTTAGTTAATTGAACAGGGCTCCCATAGTGGCTCAATGTGATAGGAAGCGAGTACTTTCCTCATGGGCACCATACTGCCGAAACTCAGCTCCATCCGAATATCAGATTCAATTTTCAAGCGTTCGTTATCTAACTCCACGAGAATTTGTTGAGAAATTATCAAAGGAGCTTGGCGTTCGTGGAGTTGTTGCAGGTAAATTAAATTAAGACGTGTAGTACAAGTCTTTGTTGATAAAATACTCTCTTGGACTTGAGTAAAGTGTGATCGTTTCGAGGCTTTGATCGACAATGGTTGCAGGTGAAAGCTATAGGTTTGGATATAAAGCAGCAGGTGATGCAGCTGAGCTAGTGAAACTGTGTGAGGAATATGGGTTAGGTGCTTATATTATAAAATCTGTGATGGACAGAAACCAAAAAGTTGTTAATTCTACCAATTCAATGGAGAGAGGACAGGTGTCATCTACTCGGGTTCGCTATGCCCTTTCCATAGGAGATATGAAGTATGTCTCTGAGCTTTTAGGCCGCAGACATCGCCTTATTTTGATGGCAAAAGGCCTAGAGGGATTAAGCAATAGCAGAGTGTCTGCCCCAAAATCATGTTTGTTAAATTTAGCACCAAAAGAAGGTCTCTATAATACGTGTTTCGTCTGTACAAGTGACGAGGATCTAATCCCATGTCGGGTAGTTATCGACTCGACTCATGTTCACATAGAAATGGATGATATAGGCACACGTCATCCCGTAGGAACACAAAACTACTTAAGTGTTGAATTTGACCATGAAGGAGCATGATCATGTTCAATTCGCCTCCTGGAATTCCTTGATGAGATCACTTATCCACAAACTAAAATGTCGGATCCCAGAGATTCTGGATTATATGCATCAGAGAGATTCATATCTATTCTTACAAAGAGGTATGGAGCTTGTTTCAGCAATTATGTTCTAAATATTTGCAGACACGAAACACGGGTCACGGGCCAATGTGGGTAATGCTTCATCATTTACAGCTGGTTCTGTGAAGAAACACTACTTATTTTGGCAGGAAAAGCTCAATAGTTCCTTTCAGCTGTCCGACAGATGCATATGACCCGAAGGTACTTTTATATGTATTAACTAAAGCACGTTGTTTGCAAGGAATTATTATATTTATGTCTCTATGTTCATTTATCTGGAAACAAATAACTCAGAGGCATCGTTTTCTGTTGGCACTGTACATGATATCTATAATTTATTTAGGGACAAATAGCTTGGAAGTTTTCTTTTTTTCAACAGATCAAAAGCTGTTGAACCCATTTTGAGTTCTAACATAAAATTTAGACCCTTTTCACTTGCTTCTTGAAATGTAAGACAGGTAGTACTGTAGCCATGTTTTCCCTTGTTGTCTTTGGTTCCCTTTTGCAAGAACATGATTTTGCTTTTGAACTTTTTACGGGCCAAAACGAACAATATCTGCTAGCGGTGAGTTTGAACTGTTACTAATGGTATTAGAGCCAGACACCGAGCAGTGTGCCAGCGGGGACGCTGGGCTCCAAGGAGGGTGGATTGTGAGATCCCACATTGATTGGAGAGAGAAACGAAACATTCCTTATAAAGGTGTGGGAATCTCTCCCTAGTAGAAGCTTCGTCCCTTTTCCTTTTGCTTGCATTCTACTTCCATGGAAATGATTTTTCTTTGGAGTGGGATGTGGAAGCAGTTGCAAAAAAAGAAAAATGTTTTTATTTTTTAAGTCGGGATATATATATATATATATGTTTTAATCAATTGGGTTATATCATTTTATTTTAGAAAATTCAATTATTAAAATTCTCTAATAATTGGTAGTTGAGGTTTTAAGAAACATGAACATGGCAGTTGGG

mRNA sequence

TGAAAGTCTGCGCCTCTCATTACTCCCCACTTGCCGCCTCCACTCGGTCCCAAGCGGCTGGCGATGTTGGCTGCCGGCGCTCGCGTTTCTCACCATCTCCGGGAGTGCGACTCTCAATTTGGCTTCGGATTAACCTCAGGCCTTACCTCCTTCCCCGTCGTTCTATTTCCTCCGATATGGTCTCGCAAGCCCACTTCTAATTTCCCTTCAATTTCTCGCCGCTCCCAACCACGTGGGGTTTCTTTCTTTTGCTCCATTATCCCCTCCACTTCCCCCGGAGAAATCCCCGTTATCTCCGATTCTTTTGGTTCACGAGAGGATGATCGTGAAGTCTCTGTGGCTGGAGGAATAGTAGCATTAGGAAAATTTGATGCTCTTCACGTTGGACATCGAGAGCTCGCAATTCAAGCTTCAACGGTTGGATCTCCATTTCTATTGTCATTCGTTGGAATTGCTGAAGTACTCGGTTGGGAACCTAGGGCTCCCATAGTGGCTCAATGTGATAGGAAGCGAGTACTTTCCTCATGGGCACCATACTGCCGAAACTCAGCTCCATCCGAATATCAGATTCAATTTTCAAGCGTTCGTTATCTAACTCCACGAGAATTTGTTGAGAAATTATCAAAGGAGCTTGGCGTTCGTGGAGTTGTTGCAGGTGAAAGCTATAGGTTTGGATATAAAGCAGCAGGTGATGCAGCTGAGCTAGTGAAACTGTGTGAGGAATATGGGTTAGGTGCTTATATTATAAAATCTGTGATGGACAGAAACCAAAAAGTTGTTAATTCTACCAATTCAATGGAGAGAGGACAGGTGTCATCTACTCGGGTTCGCTATGCCCTTTCCATAGGAGATATGAAGTATGTCTCTGAGCTTTTAGGCCGCAGACATCGCCTTATTTTGATGGCAAAAGGCCTAGAGGGATTAAGCAATAGCAGAGTGTCTGCCCCAAAATCATGTTTGTTAAATTTAGCACCAAAAGAAGGTCTCTATAATACGTGTTTCGTCTGTACAAGTGACGAGGATCTAATCCCATGTCGGGTAGTTATCGACTCGACTCATGTTCACATAGAAATGGATGATATAGGCACACGTCATCCCGTAGGAACACAAAACTACTTAAGTGTTGAATTTGACCATGAAGGAGCATGATCATGTTCAATTCGCCTCCTGGAATTCCTTGATGAGATCACTTATCCACAAACTAAAATGTCGGATCCCAGAGATTCTGGATTATATGCATCAGAGAGATTCATATCTATTCTTACAAAGAGGTATGGAGCTTGTTTCAGCAATTATGTTCTAAATATTTGCAGACACGAAACACGGGTCACGGGCCAATGTGGGTAATGCTTCATCATTTACAGCTGGTTCTGTGAAGAAACACTACTTATTTTGGCAGGAAAAGCTCAATAGTTCCTTTCAGCTGTCCGACAGATGCATATGACCCGAAGGTACTTTTATATGTATTAACTAAAGCACGTTGTTTGCAAGGAATTATTATATTTATGTCTCTATGTTCATTTATCTGGAAACAAATAACTCAGAGGCATCGTTTTCTGTTGGCACTGTACATGATATCTATAATTTATTTAGGGACAAATAGCTTGGAAGTTTTCTTTTTTTCAACAGATCAAAAGCTGTTGAACCCATTTTGAGTTCTAACATAAAATTTAGACCCTTTTCACTTGCTTCTTGAAATGTAAGACAGGTAGTACTGTAGCCATGTTTTCCCTTGTTGTCTTTGGTTCCCTTTTGCAAGAACATGATTTTGCTTTTGAACTTTTTACGGGCCAAAACGAACAATATCTGCTAGCGGTGAGTTTGAACTGTTACTAATGGTATTAGAGCCAGACACCGAGCAGTGTGCCAGCGGGGACGCTGGGCTCCAAGGAGGGTGGATTGTGAGATCCCACATTGATTGGAGAGAGAAACGAAACATTCCTTATAAAGGTGTGGGAATCTCTCCCTAGTAGAAGCTTCGTCCCTTTTCCTTTTGCTTGCATTCTACTTCCATGGAAATGATTTTTCTTTGGAGTGGGATGTGGAAGCAGTTGCAAAAAAAGAAAAATGTTTTTATTTTTTAAGTCGGGATATATATATATATATATGTTTTAATCAATTGGGTTATATCATTTTATTTTAGAAAATTCAATTATTAAAATTCTCTAATAATTGGTAGTTGAGGTTTTAAGAAACATGAACATGGCAGTTGGG

Coding sequence (CDS)

ATGTTGGCTGCCGGCGCTCGCGTTTCTCACCATCTCCGGGAGTGCGACTCTCAATTTGGCTTCGGATTAACCTCAGGCCTTACCTCCTTCCCCGTCGTTCTATTTCCTCCGATATGGTCTCGCAAGCCCACTTCTAATTTCCCTTCAATTTCTCGCCGCTCCCAACCACGTGGGGTTTCTTTCTTTTGCTCCATTATCCCCTCCACTTCCCCCGGAGAAATCCCCGTTATCTCCGATTCTTTTGGTTCACGAGAGGATGATCGTGAAGTCTCTGTGGCTGGAGGAATAGTAGCATTAGGAAAATTTGATGCTCTTCACGTTGGACATCGAGAGCTCGCAATTCAAGCTTCAACGGTTGGATCTCCATTTCTATTGTCATTCGTTGGAATTGCTGAAGTACTCGGTTGGGAACCTAGGGCTCCCATAGTGGCTCAATGTGATAGGAAGCGAGTACTTTCCTCATGGGCACCATACTGCCGAAACTCAGCTCCATCCGAATATCAGATTCAATTTTCAAGCGTTCGTTATCTAACTCCACGAGAATTTGTTGAGAAATTATCAAAGGAGCTTGGCGTTCGTGGAGTTGTTGCAGGTGAAAGCTATAGGTTTGGATATAAAGCAGCAGGTGATGCAGCTGAGCTAGTGAAACTGTGTGAGGAATATGGGTTAGGTGCTTATATTATAAAATCTGTGATGGACAGAAACCAAAAAGTTGTTAATTCTACCAATTCAATGGAGAGAGGACAGGTGTCATCTACTCGGGTTCGCTATGCCCTTTCCATAGGAGATATGAAGTATGTCTCTGAGCTTTTAGGCCGCAGACATCGCCTTATTTTGATGGCAAAAGGCCTAGAGGGATTAAGCAATAGCAGAGTGTCTGCCCCAAAATCATGTTTGTTAAATTTAGCACCAAAAGAAGGTCTCTATAATACGTGTTTCGTCTGTACAAGTGACGAGGATCTAATCCCATGTCGGGTAGTTATCGACTCGACTCATGTTCACATAGAAATGGATGATATAGGCACACGTCATCCCGTAGGAACACAAAACTACTTAAGTGTTGAATTTGACCATGAAGGAGCATGA

Protein sequence

MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVSFFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVNSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSRVSAPKSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDHEGA
Homology
BLAST of CmoCh04G013340 vs. ExPASy Swiss-Prot
Match: Q8VZR0 (FAD synthetase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF2 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.7e-99
Identity = 194/368 (52.72%), Postives = 255/368 (69.29%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPS--ISRRSQPRG 60
           ML  G+RV  HL + +     GL  G     +V     + R   +   S   SR+ + R 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGFCGAKIVQLSSFFLRPSQAMAKSHHFSRKLRQRM 60

Query: 61  VSFFCSIIPSTSPGEIPVISDSFGSREDDREV------SVAGGIVALGKFDALHVGHREL 120
           +S F S     + GE+P++ + F  REDD E+       V+GGIVALGKFDALH+GHREL
Sbjct: 61  ISSFGS--HCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHREL 120

Query: 121 AIQASTVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFS 180
            IQAS +G+P+LLSFVG+AEVLGWEPRAPIVA+CDR+RVL+SWA YC + AP EY+I+F+
Sbjct: 121 TIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIEFA 180

Query: 181 SVRYLTPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVM 240
           SVR+LTPR+FVEKLSKEL V GVVAGE+YRFGYKA+GDA+ELV+LCEE G+ A II SVM
Sbjct: 181 SVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINSVM 240

Query: 241 DRNQKVV--NSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNS 300
           D  Q     +S +S +RGQVSSTRVR AL+ GDM+YVSELLGR HRLIL  +  +  S  
Sbjct: 241 DMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQDMPSER 300

Query: 301 RVSAPKSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQN 357
            +S P+S +LNL P  G+Y  C +   DE  +PC VV+D++++H+E +++   +   +Q 
Sbjct: 301 MISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEEVRLCNLDWSQE 360

BLAST of CmoCh04G013340 vs. ExPASy Swiss-Prot
Match: Q10BX9 (FAD synthetase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBF PE=3 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.6e-88
Identity = 165/270 (61.11%), Postives = 206/270 (76.30%), Query Frame = 0

Query: 92  VAGGIVALGKFDALHVGHRELAIQASTVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRV 151
           V  GIVALGKFDALH+GHRELA+ AS  G+PFLLSFVGIAEVLGWE R PIVAQCDRKRV
Sbjct: 122 VIDGIVALGKFDALHIGHRELAMYASKAGTPFLLSFVGIAEVLGWEYRPPIVAQCDRKRV 181

Query: 152 LSSWAPYCRNSAPSEYQIQFSSVRYLTPREFVEKLSKELGVRGVVAGESYRFGYKAAGDA 211
           L+SWAPYC+N  P EYQ++FS VRYLTPR+FVE+LS++L ++GVVAGE+YRFGY+A+GDA
Sbjct: 182 LTSWAPYCKNVVPIEYQVEFSKVRYLTPRQFVERLSRDLKIQGVVAGENYRFGYRASGDA 241

Query: 212 AELVKLCEEYGLGAYIIKSVMDRNQK---VVNSTNSMERGQVSSTRVRYALSIGDMKYVS 271
           AELVKLCEE+GL A+I++SVMD  +    V  S NS ++GQVSS+RVR+AL++GDM+YVS
Sbjct: 242 AELVKLCEEFGLSAFIVRSVMDTARSYNGVTTSVNSSDKGQVSSSRVRHALAMGDMEYVS 301

Query: 272 ELLGRRHRLILMAKGLEGLSNSRVSAPKSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVI 331
           ELLGR+HRL+L  K        R+  PKSC+LN+ P +GLY  C +      L  CRV+I
Sbjct: 302 ELLGRKHRLVLTVKENHLQERKRIMLPKSCMLNMPPADGLYENCDLLNGGH-LGLCRVII 361

Query: 332 DSTHVHIEMDDIGTRHP--VGTQNYLSVEF 357
           +S  + IEM D  +  P  +     L +EF
Sbjct: 362 NSETIVIEMKDENSLLPNTIQENQQLGIEF 390

BLAST of CmoCh04G013340 vs. ExPASy Swiss-Prot
Match: Q9FMW8 (FAD synthetase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF1 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.0e-88
Identity = 177/329 (53.80%), Postives = 236/329 (71.73%), Query Frame = 0

Query: 41  RKPTSNFPSISRRSQPRGVSFFCSIIPSTSPGEIPVISDSFGSREDDREVS------VAG 100
           RK +      S  SQ R  S F S     +P +IP   D F   +D  E+S      VAG
Sbjct: 27  RKSSFMLRPCSAISQQRIKSSFRS--HCKTPRKIPAPLDCFSQGDDHPELSAEGLSPVAG 86

Query: 101 GIVALGKFDALHVGHRELAIQASTVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSS 160
           GIVALGKFDALH+GHRELAIQA+ +G+P+LLSFVG+AEVLGW+PRAPIVA+CDRKRVLSS
Sbjct: 87  GIVALGKFDALHIGHRELAIQAARIGTPYLLSFVGLAEVLGWKPRAPIVAKCDRKRVLSS 146

Query: 161 WAPYCRNSAPSEYQIQFSSVRYLTPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAEL 220
           WA YC N AP E++I+F+SVR+L P++FVEKLS+EL V GVVAGE+YRFGY+A+GDA+EL
Sbjct: 147 WASYCGNIAPVEFEIEFASVRHLNPQQFVEKLSRELRVCGVVAGENYRFGYRASGDASEL 206

Query: 221 VKLCEEYGLGAYIIKSVMDRNQKVVNS----TNSMERGQVSSTRVRYALSIGDMKYVSEL 280
           V+LC+++G+ AYII SVMD+NQ  VN+    + S ERGQVSSTRVR+AL+ GD++YV+EL
Sbjct: 207 VRLCKDFGISAYIINSVMDKNQVSVNTEEEDSKSKERGQVSSTRVRHALAAGDVRYVTEL 266

Query: 281 LGRRHRLILMAKGLEGLS-NSRVSAPKSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVID 340
           LGR HR+I   +  +  S   R+S   S LLNL P  G+Y  C +   D+  I C+V++D
Sbjct: 267 LGRPHRVISRTRTQDLTSKRGRISLQTSSLLNLPPGNGVYKACSLIVGDKHPISCKVIVD 326

Query: 341 STHVHIEMDDIGTRHPVGTQNY--LSVEF 357
           +++++IE ++    +   +Q +  L +EF
Sbjct: 327 TSNLYIETEEERFHNSDESQEFQLLGIEF 353

BLAST of CmoCh04G013340 vs. ExPASy Swiss-Prot
Match: P57250 (Bifunctional riboflavin kinase/FMN adenylyltransferase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=ribF PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.0e-07
Identity = 52/194 (26.80%), Postives = 95/194 (48.97%), Query Frame = 0

Query: 93  AGGIVALGKFDALHVGHRELAIQASTVGSPFLLSFVGIAEVLGWEPR---------APIV 152
           +  +V +G FD +H+GH++L      +G  + LS +    V+ +EP+         AP+ 
Sbjct: 15  SNSVVTIGNFDGIHLGHQKLFSHIYQIGQKYKLSTI----VVLFEPQPLEFLRKNNAPVR 74

Query: 153 AQCDRKRV--LSSWAPYCRNSAPSEYQIQFS-SVRYLTPREF-VEKLSKELGVRGVVAGE 212
               R+++  +SS+      +  S   ++F+ S + L+ ++F +  L  +L ++ +V G 
Sbjct: 75  ITKFREKIRRISSY------NFDSILCVKFNKSFQSLSAKDFIINILINKLHLKFIVIGN 134

Query: 213 SYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVNSTNSMERGQVSSTRVRYAL 272
            +RFG++  G+   L KL  +Y      I+ +   N K            +SST +R AL
Sbjct: 135 DFRFGFQRNGNINLLKKLGYKYQFNVIKIRPLYKNNIK------------ISSTNIRKAL 186

Query: 273 SIGDMKYVSELLGR 274
           S  ++K  S LLGR
Sbjct: 195 SENNIKLASLLLGR 186

BLAST of CmoCh04G013340 vs. ExPASy TrEMBL
Match: A0A6J1GWB8 (FAD synthase OS=Cucurbita moschata OX=3662 GN=LOC111458123 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 1.2e-205
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSRVSAPKSCLL 300
           STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSRVSAPKSCLL
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSRVSAPKSCLL 300

Query: 301 NLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDHEG 360
           NLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDHEG
Sbjct: 301 NLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDHEG 360

Query: 361 A 362
           A
Sbjct: 361 A 361

BLAST of CmoCh04G013340 vs. ExPASy TrEMBL
Match: A0A6J1KGG1 (FAD synthase OS=Cucurbita maxima OX=3661 GN=LOC111493664 PE=4 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.1e-201
Identity = 356/365 (97.53%), Postives = 358/365 (98.08%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSIIPSTSPGEIPVISDS GSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSVGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPR PIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRPPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL----SNSRVSAPK 300
           STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL    SNSR+SAPK
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRLSAPK 300

Query: 301 SCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360
           SCLLNLAPKEGLYN CFVCTSD+DLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF
Sbjct: 301 SCLLNLAPKEGLYNKCFVCTSDDDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360

Query: 361 DHEGA 362
           DHEGA
Sbjct: 361 DHEGA 365

BLAST of CmoCh04G013340 vs. ExPASy TrEMBL
Match: A0A5A7STW7 (FAD synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00660 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.7e-176
Identity = 314/358 (87.71%), Postives = 330/358 (92.18%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAA  RVSHHLRE DS F FGLTSGLTS  ++LFPPIW R PTSNFPSIS RSQPR VS
Sbjct: 1   MLAASVRVSHHLREFDSHFAFGLTSGLTSSAILLFPPIWPRNPTSNFPSISHRSQPRRVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSI+PSTS GEIPV+SD FGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS VG
Sbjct: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYC+NSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKEL V GVVAGESYRFGYKAAGDAAELVKLCEEYG+ AYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLS--NSRVSAPKSC 300
           S NS ERGQVSSTRVR+ALSIGDMKYVSELLGRRHRLILMAKGLEG S  NSRVSAP+SC
Sbjct: 241 SANSKERGQVSSTRVRHALSIGDMKYVSELLGRRHRLILMAKGLEGFSNTNSRVSAPRSC 300

Query: 301 LLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 357
           LLNL+PKEG YN CF+CT+DE+LIPCRV IDSTHVHIEMDDIGT H VGTQ+ +++EF
Sbjct: 301 LLNLSPKEGFYNNCFLCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDCVNIEF 358

BLAST of CmoCh04G013340 vs. ExPASy TrEMBL
Match: A0A1S3BD91 (FAD synthase OS=Cucumis melo OX=3656 GN=LOC103488775 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.7e-176
Identity = 314/358 (87.71%), Postives = 330/358 (92.18%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAA  RVSHHLRE DS F FGLTSGLTS  ++LFPPIW R PTSNFPSIS RSQPR VS
Sbjct: 1   MLAASVRVSHHLREFDSHFAFGLTSGLTSSAILLFPPIWPRNPTSNFPSISHRSQPRRVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSI+PSTS GEIPV+SD FGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS VG
Sbjct: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYC+NSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKEL V GVVAGESYRFGYKAAGDAAELVKLCEEYG+ AYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLS--NSRVSAPKSC 300
           S NS ERGQVSSTRVR+ALSIGDMKYVSELLGRRHRLILMAKGLEG S  NSRVSAP+SC
Sbjct: 241 SANSKERGQVSSTRVRHALSIGDMKYVSELLGRRHRLILMAKGLEGFSNTNSRVSAPRSC 300

Query: 301 LLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 357
           LLNL+PKEG YN CF+CT+DE+LIPCRV IDSTHVHIEMDDIGT H VGTQ+ +++EF
Sbjct: 301 LLNLSPKEGFYNNCFLCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDCVNIEF 358

BLAST of CmoCh04G013340 vs. ExPASy TrEMBL
Match: A0A0A0KPQ8 (FAD synthase OS=Cucumis sativus OX=3659 GN=Csa_5G577970 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.6e-175
Identity = 314/361 (86.98%), Postives = 330/361 (91.41%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAAG RVSHHLR+ DS F FGLTSGL S   +LFPPIW R PTSNFPSIS RSQPR VS
Sbjct: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLASSAFLLFPPIWPRNPTSNFPSISHRSQPRRVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSI+PSTS GEIPV+SD FGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS VG
Sbjct: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDR+RVLSSWAPYC+NSAPSEY+IQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRQRVLSSWAPYCQNSAPSEYRIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKEL V GVVAGESYRFGYKAAGDAAELVKLCEEYG+ AYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNS--RVSAPKSC 300
           S NS ERGQVSSTRVR+ALS GDMKYVSELLGRRHRLILMA+GLEG SNS  RVSAP+SC
Sbjct: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300

Query: 301 LLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDH 360
           LLNLAPKEGLYN CFVCT+DE+LIPCRV IDSTHVHIEMDDIGT H VGTQ+ ++VEF  
Sbjct: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360

BLAST of CmoCh04G013340 vs. NCBI nr
Match: XP_022956361.1 (FAD synthetase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 725.3 bits (1871), Expect = 2.6e-205
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSRVSAPKSCLL 300
           STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSRVSAPKSCLL
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSRVSAPKSCLL 300

Query: 301 NLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDHEG 360
           NLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDHEG
Sbjct: 301 NLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDHEG 360

Query: 361 A 362
           A
Sbjct: 361 A 361

BLAST of CmoCh04G013340 vs. NCBI nr
Match: XP_023524438.1 (FAD synthetase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 713.4 bits (1840), Expect = 1.0e-201
Identity = 357/365 (97.81%), Postives = 359/365 (98.36%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMD NQKVVN
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDTNQKVVN 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL----SNSRVSAPK 300
           STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL    SNSRVSAPK
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRVSAPK 300

Query: 301 SCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360
           SCLLNLAPKEGLYN CF+CTSD+DLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF
Sbjct: 301 SCLLNLAPKEGLYNKCFICTSDDDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360

Query: 361 DHEGA 362
           DHEGA
Sbjct: 361 DHEGA 365

BLAST of CmoCh04G013340 vs. NCBI nr
Match: KAG6601065.1 (FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 712.2 bits (1837), Expect = 2.2e-201
Identity = 357/365 (97.81%), Postives = 359/365 (98.36%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSR PTSNFPSISRRSQPRGVS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRNPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVV+
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVD 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL----SNSRVSAPK 300
           STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL    SNSRVSAPK
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRVSAPK 300

Query: 301 SCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360
           SCLLNLAPKEGLYN CFVCTSD+DLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF
Sbjct: 301 SCLLNLAPKEGLYNKCFVCTSDDDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360

Query: 361 DHEGA 362
           DHEGA
Sbjct: 361 DHEGA 365

BLAST of CmoCh04G013340 vs. NCBI nr
Match: XP_022999229.1 (FAD synthetase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 710.3 bits (1832), Expect = 8.5e-201
Identity = 356/365 (97.53%), Postives = 358/365 (98.08%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSIIPSTSPGEIPVISDS GSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSVGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPR PIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRPPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL----SNSRVSAPK 300
           STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL    SNSR+SAPK
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRLSAPK 300

Query: 301 SCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360
           SCLLNLAPKEGLYN CFVCTSD+DLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF
Sbjct: 301 SCLLNLAPKEGLYNKCFVCTSDDDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360

Query: 361 DHEGA 362
           DHEGA
Sbjct: 361 DHEGA 365

BLAST of CmoCh04G013340 vs. NCBI nr
Match: KAG7031874.1 (FAD synthetase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 705.3 bits (1819), Expect = 2.7e-199
Identity = 355/365 (97.26%), Postives = 357/365 (97.81%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60
           MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLF PIWSR PTSNF SISRRSQPRGVS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFHPIWSRNPTSNFRSISRRSQPRGVS 60

Query: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120
           FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240
           EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVV+
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVD 240

Query: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL----SNSRVSAPK 300
           STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL    SNSRVSAPK
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRVSAPK 300

Query: 301 SCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360
           SCLLNLAPKEGLYN CFVCTSD+DLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF
Sbjct: 301 SCLLNLAPKEGLYNKCFVCTSDDDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360

Query: 361 DHEGA 362
           DHEGA
Sbjct: 361 DHEGA 365

BLAST of CmoCh04G013340 vs. TAIR 10
Match: AT5G08340.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 6.9e-100
Identity = 194/368 (52.72%), Postives = 255/368 (69.29%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPS--ISRRSQPRG 60
           ML  G+RV  HL + +     GL  G     +V     + R   +   S   SR+ + R 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGFCGAKIVQLSSFFLRPSQAMAKSHHFSRKLRQRM 60

Query: 61  VSFFCSIIPSTSPGEIPVISDSFGSREDDREV------SVAGGIVALGKFDALHVGHREL 120
           +S F S     + GE+P++ + F  REDD E+       V+GGIVALGKFDALH+GHREL
Sbjct: 61  ISSFGS--HCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHREL 120

Query: 121 AIQASTVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFS 180
            IQAS +G+P+LLSFVG+AEVLGWEPRAPIVA+CDR+RVL+SWA YC + AP EY+I+F+
Sbjct: 121 TIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIEFA 180

Query: 181 SVRYLTPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVM 240
           SVR+LTPR+FVEKLSKEL V GVVAGE+YRFGYKA+GDA+ELV+LCEE G+ A II SVM
Sbjct: 181 SVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINSVM 240

Query: 241 DRNQKVV--NSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNS 300
           D  Q     +S +S +RGQVSSTRVR AL+ GDM+YVSELLGR HRLIL  +  +  S  
Sbjct: 241 DMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQDMPSER 300

Query: 301 RVSAPKSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQN 357
            +S P+S +LNL P  G+Y  C +   DE  +PC VV+D++++H+E +++   +   +Q 
Sbjct: 301 MISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEEVRLCNLDWSQE 360

BLAST of CmoCh04G013340 vs. TAIR 10
Match: AT5G08340.2 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 358.2 bits (918), Expect = 7.6e-99
Identity = 189/349 (54.15%), Postives = 245/349 (70.20%), Query Frame = 0

Query: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPS--ISRRSQPRG 60
           ML  G+RV  HL + +     GL  G     +V     + R   +   S   SR+ + R 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGFCGAKIVQLSSFFLRPSQAMAKSHHFSRKLRQRM 60

Query: 61  VSFFCSIIPSTSPGEIPVISDSFGSREDDREV------SVAGGIVALGKFDALHVGHREL 120
           +S F S     + GE+P++ + F  REDD E+       V+GGIVALGKFDALH+GHREL
Sbjct: 61  ISSFGS--HCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHREL 120

Query: 121 AIQASTVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFS 180
            IQAS +G+P+LLSFVG+AEVLGWEPRAPIVA+CDR+RVL+SWA YC + AP EY+I+F+
Sbjct: 121 TIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIEFA 180

Query: 181 SVRYLTPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVM 240
           SVR+LTPR+FVEKLSKEL V GVVAGE+YRFGYKA+GDA+ELV+LCEE G+ A II SVM
Sbjct: 181 SVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINSVM 240

Query: 241 DRNQKVV--NSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNS 300
           D  Q     +S +S +RGQVSSTRVR AL+ GDM+YVSELLGR HRLIL  +  +  S  
Sbjct: 241 DMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQDMPSER 300

Query: 301 RVSAPKSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDD 340
            +S P+S +LNL P  G+Y  C +   DE  +PC VV+D++++H+E ++
Sbjct: 301 MISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEE 347

BLAST of CmoCh04G013340 vs. TAIR 10
Match: AT5G23330.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.4e-89
Identity = 177/329 (53.80%), Postives = 236/329 (71.73%), Query Frame = 0

Query: 41  RKPTSNFPSISRRSQPRGVSFFCSIIPSTSPGEIPVISDSFGSREDDREVS------VAG 100
           RK +      S  SQ R  S F S     +P +IP   D F   +D  E+S      VAG
Sbjct: 27  RKSSFMLRPCSAISQQRIKSSFRS--HCKTPRKIPAPLDCFSQGDDHPELSAEGLSPVAG 86

Query: 101 GIVALGKFDALHVGHRELAIQASTVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSS 160
           GIVALGKFDALH+GHRELAIQA+ +G+P+LLSFVG+AEVLGW+PRAPIVA+CDRKRVLSS
Sbjct: 87  GIVALGKFDALHIGHRELAIQAARIGTPYLLSFVGLAEVLGWKPRAPIVAKCDRKRVLSS 146

Query: 161 WAPYCRNSAPSEYQIQFSSVRYLTPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAEL 220
           WA YC N AP E++I+F+SVR+L P++FVEKLS+EL V GVVAGE+YRFGY+A+GDA+EL
Sbjct: 147 WASYCGNIAPVEFEIEFASVRHLNPQQFVEKLSRELRVCGVVAGENYRFGYRASGDASEL 206

Query: 221 VKLCEEYGLGAYIIKSVMDRNQKVVNS----TNSMERGQVSSTRVRYALSIGDMKYVSEL 280
           V+LC+++G+ AYII SVMD+NQ  VN+    + S ERGQVSSTRVR+AL+ GD++YV+EL
Sbjct: 207 VRLCKDFGISAYIINSVMDKNQVSVNTEEEDSKSKERGQVSSTRVRHALAAGDVRYVTEL 266

Query: 281 LGRRHRLILMAKGLEGLS-NSRVSAPKSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVID 340
           LGR HR+I   +  +  S   R+S   S LLNL P  G+Y  C +   D+  I C+V++D
Sbjct: 267 LGRPHRVISRTRTQDLTSKRGRISLQTSSLLNLPPGNGVYKACSLIVGDKHPISCKVIVD 326

Query: 341 STHVHIEMDDIGTRHPVGTQNY--LSVEF 357
           +++++IE ++    +   +Q +  L +EF
Sbjct: 327 TSNLYIETEEERFHNSDESQEFQLLGIEF 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZR09.7e-9952.72FAD synthetase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF2 PE=1 SV... [more]
Q10BX91.6e-8861.11FAD synthetase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBF P... [more]
Q9FMW82.0e-8853.80FAD synthetase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF1 PE=1 SV... [more]
P572503.0e-0726.80Bifunctional riboflavin kinase/FMN adenylyltransferase OS=Buchnera aphidicola su... [more]
Match NameE-valueIdentityDescription
A0A6J1GWB81.2e-205100.00FAD synthase OS=Cucurbita moschata OX=3662 GN=LOC111458123 PE=4 SV=1[more]
A0A6J1KGG14.1e-20197.53FAD synthase OS=Cucurbita maxima OX=3661 GN=LOC111493664 PE=4 SV=1[more]
A0A5A7STW72.7e-17687.71FAD synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00660 PE... [more]
A0A1S3BD912.7e-17687.71FAD synthase OS=Cucumis melo OX=3656 GN=LOC103488775 PE=4 SV=1[more]
A0A0A0KPQ86.6e-17586.98FAD synthase OS=Cucumis sativus OX=3659 GN=Csa_5G577970 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022956361.12.6e-205100.00FAD synthetase 2, chloroplastic-like [Cucurbita moschata][more]
XP_023524438.11.0e-20197.81FAD synthetase 2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6601065.12.2e-20197.81FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022999229.18.5e-20197.53FAD synthetase 2, chloroplastic-like [Cucurbita maxima][more]
KAG7031874.12.7e-19997.26FAD synthetase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G08340.16.9e-10052.72Nucleotidylyl transferase superfamily protein [more]
AT5G08340.27.6e-9954.15Nucleotidylyl transferase superfamily protein [more]
AT5G23330.11.4e-8953.80Nucleotidylyl transferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 83..274
e-value: 8.5E-34
score: 118.7
IPR015864FAD synthetasePFAMPF06574FAD_syncoord: 174..239
e-value: 1.3E-9
score: 38.1
NoneNo IPR availablePANTHERPTHR12714:SF20FAD SYNTHETASE 1, CHLOROPLASTIC-RELATEDcoord: 6..357
NoneNo IPR availablePANTHERPTHR12714PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASEcoord: 6..357
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 96..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G013340.1CmoCh04G013340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006747 FAD biosynthetic process
biological_process GO:0009231 riboflavin biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0003919 FMN adenylyltransferase activity